                         SEQUENCE LISTING

<110>  AELAN CELL TECHNOLOGIES, INC.
       Lunyak, Victoria
       Tollervey, James Robert
 
<120>  COMPOSITIONS AND METHODS RELATED TO THE METHYLATION OF HISTONE H1.0 PROTEIN

<130>  ALNC-006/01WO 328515-2017

<140>  PCT/US2017/028653
<141>  2017-04-20

<150>  US 62/325,392
<151>  2016-04-20

<150>  US 62/325,408
<151>  2016-04-20

<150>  US 62/325,362
<151>  2016-04-20

<150>  US 62/355,265
<151>  2016-06-27

<150>  US 62/355,277
<151>  2016-06-27

<160>  97    

<170>  PatentIn version 3.5

<210>  1
<211>  194
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Thr Glu Asn Ser Thr Ser Ala Pro Ala Ala Lys Pro Lys Arg Ala 
1               5                   10                  15      


Lys Ala Ser Lys Lys Ser Thr Asp His Pro Lys Tyr Ser Asp Met Ile 
            20                  25                  30          


Val Ala Ala Ile Gln Ala Glu Lys Asn Arg Ala Gly Ser Ser Arg Gln 
        35                  40                  45              


Ser Ile Gln Lys Tyr Ile Lys Ser His Tyr Lys Val Gly Glu Asn Ala 
    50                  55                  60                  


Asp Ser Gln Ile Lys Leu Ser Ile Lys Arg Leu Val Thr Thr Gly Val 
65                  70                  75                  80  


Leu Lys Gln Thr Lys Gly Val Gly Ala Ser Gly Ser Phe Arg Leu Ala 
                85                  90                  95      


Lys Ser Asp Glu Pro Lys Lys Ser Val Ala Phe Lys Lys Thr Lys Lys 
            100                 105                 110         


Glu Ile Lys Lys Val Ala Thr Pro Lys Lys Ala Ser Lys Pro Lys Lys 
        115                 120                 125             


Ala Ala Ser Lys Ala Pro Thr Lys Lys Pro Lys Ala Thr Pro Val Lys 
    130                 135                 140                 


Lys Ala Lys Lys Lys Leu Ala Ala Thr Pro Lys Lys Ala Lys Lys Pro 
145                 150                 155                 160 


Lys Thr Val Lys Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala 
                165                 170                 175     


Lys Pro Val Lys Pro Lys Ala Lys Ser Ser Ala Lys Arg Ala Gly Lys 
            180                 185                 190         


Lys Lys 
        


<210>  2
<211>  194
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  Human H1.0 protein comprising a dimethylated K180


<220>
<221>  misc_feature
<222>  (180)..(180)
<223>  Lys is dimethylated

<400>  2

Met Thr Glu Asn Ser Thr Ser Ala Pro Ala Ala Lys Pro Lys Arg Ala 
1               5                   10                  15      


Lys Ala Ser Lys Lys Ser Thr Asp His Pro Lys Tyr Ser Asp Met Ile 
            20                  25                  30          


Val Ala Ala Ile Gln Ala Glu Lys Asn Arg Ala Gly Ser Ser Arg Gln 
        35                  40                  45              


Ser Ile Gln Lys Tyr Ile Lys Ser His Tyr Lys Val Gly Glu Asn Ala 
    50                  55                  60                  


Asp Ser Gln Ile Lys Leu Ser Ile Lys Arg Leu Val Thr Thr Gly Val 
65                  70                  75                  80  


Leu Lys Gln Thr Lys Gly Val Gly Ala Ser Gly Ser Phe Arg Leu Ala 
                85                  90                  95      


Lys Ser Asp Glu Pro Lys Lys Ser Val Ala Phe Lys Lys Thr Lys Lys 
            100                 105                 110         


Glu Ile Lys Lys Val Ala Thr Pro Lys Lys Ala Ser Lys Pro Lys Lys 
        115                 120                 125             


Ala Ala Ser Lys Ala Pro Thr Lys Lys Pro Lys Ala Thr Pro Val Lys 
    130                 135                 140                 


Lys Ala Lys Lys Lys Leu Ala Ala Thr Pro Lys Lys Ala Lys Lys Pro 
145                 150                 155                 160 


Lys Thr Val Lys Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala 
                165                 170                 175     


Lys Pro Val Lys Pro Lys Ala Lys Ser Ser Ala Lys Arg Ala Gly Lys 
            180                 185                 190         


Lys Lys 
        


<210>  3
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Lys is dimethylated

<400>  3

Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10                  15      


Pro Lys 
        


<210>  4
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Lys is dimethylated

<400>  4

Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10                  15      


Lys 
    


<210>  5
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Lys is dimethylated

<400>  5

Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   10                  15      


<210>  6
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Lys is dimethylated

<400>  6

Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   10                  15  


<210>  7
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Lys is dimethylated

<400>  7

Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   10                  


<210>  8
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Lys is dimethylated

<400>  8

Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   10              


<210>  9
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Lys is dimethylated

<400>  9

Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   10          


<210>  10
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Lys is dimethylated

<400>  10

Lys Pro Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   10      


<210>  11
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Lys is dimethylated

<400>  11

Pro Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   10  


<210>  12
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Lys is dimethylated

<400>  12

Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   


<210>  13
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Lys is dimethylated

<400>  13

Lys Ala Lys Pro Val Lys Pro Lys 
1               5               


<210>  14
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Lys is dimethylated

<400>  14

Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10                  15      


Pro 
    


<210>  15
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Lys is dimethylated

<400>  15

Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10                  15      


<210>  16
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Lys is dimethylated

<400>  16

Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10                  15  


<210>  17
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Lys is dimethylated

<400>  17

Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10                  


<210>  18
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Lys is dimethylated

<400>  18

Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10              


<210>  19
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Lys is dimethylated

<400>  19

Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10          


<210>  20
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Lys is dimethylated

<400>  20

Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10      


<210>  21
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Lys is dimethylated

<400>  21

Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10  


<210>  22
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Lys is dimethylated

<400>  22

Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   


<210>  23
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Lys is dimethylated

<400>  23

Lys Lys Ala Lys Pro Val Lys Pro 
1               5               


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Lys is dimethylated

<400>  24

Lys Ala Lys Pro Val Lys Pro 
1               5           


<210>  25
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Lys is dimethylated

<400>  25

Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10                  15      


<210>  26
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Lys is dimethylated

<400>  26

Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10                  15  


<210>  27
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Lys is dimethylated

<400>  27

Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10                  


<210>  28
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Lys is dimethylated

<400>  28

Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10              


<210>  29
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Lys is dimethylated

<400>  29

Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10          


<210>  30
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Lys is dimethylated

<400>  30

Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10      


<210>  31
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Lys is dimethylated

<400>  31

Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10  


<210>  32
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Lys is dimethylated

<400>  32

Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   


<210>  33
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Lys is dimethylated

<400>  33

Pro Lys Lys Ala Lys Pro Val Lys 
1               5               


<210>  34
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Lys is dimethylated

<400>  34

Lys Lys Ala Lys Pro Val Lys 
1               5           


<210>  35
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Lys is dimethylated

<400>  35

Lys Ala Lys Pro Val Lys 
1               5       


<210>  36
<211>  18
<212>  PRT
<213>  Homo sapiens

<400>  36

Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10                  15      


Pro Lys 
        


<210>  37
<211>  17
<212>  PRT
<213>  Homo sapiens

<400>  37

Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10                  15      


Lys 
    


<210>  38
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  38

Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   10                  15      


<210>  39
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  39

Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   10                  15  


<210>  40
<211>  14
<212>  PRT
<213>  Homo sapiens

<400>  40

Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   10                  


<210>  41
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  41

Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   10              


<210>  42
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  42

Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   10          


<210>  43
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  43

Lys Pro Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   10      


<210>  44
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  44

Pro Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   10  


<210>  45
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  45

Lys Lys Ala Lys Pro Val Lys Pro Lys 
1               5                   


<210>  46
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  46

Lys Ala Lys Pro Val Lys Pro Lys 
1               5               


<210>  47
<211>  17
<212>  PRT
<213>  Homo sapiens

<400>  47

Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10                  15      


Pro 
    


<210>  48
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  48

Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10                  15      


<210>  49
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  49

Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10                  15  


<210>  50
<211>  14
<212>  PRT
<213>  Homo sapiens

<400>  50

Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10                  


<210>  51
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  51

Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10              


<210>  52
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  52

Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10          


<210>  53
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  53

Ser Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10      


<210>  54
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  54

Lys Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   10  


<210>  55
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  55

Pro Lys Lys Ala Lys Pro Val Lys Pro 
1               5                   


<210>  56
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  56

Lys Lys Ala Lys Pro Val Lys Pro 
1               5               


<210>  57
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  57

Lys Ala Lys Pro Val Lys Pro 
1               5           


<210>  58
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  58

Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10                  15      


<210>  59
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  59

Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10                  15  


<210>  60
<211>  14
<212>  PRT
<213>  Homo sapiens

<400>  60

Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10                  


<210>  61
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  61

Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10              


<210>  62
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  62

Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10          


<210>  63
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  63

Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10      


<210>  64
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  64

Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10  


<210>  65
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  65

Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   


<210>  66
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  66

Pro Lys Lys Ala Lys Pro Val Lys 
1               5               


<210>  67
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  67

Lys Lys Ala Lys Pro Val Lys 
1               5           


<210>  68
<211>  6
<212>  PRT
<213>  Homo sapiens

<400>  68

Lys Ala Lys Pro Val Lys 
1               5       


<210>  69
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  biotinylated H1.0 peptide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Ala is biotinylated

<400>  69

Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10                  15      


Pro Lys 
        


<210>  70
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  biotinylated H1.0 dimethylated peptide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Ala is biotinylated

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Lys is dimethylated

<400>  70

Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10                  15      


Pro Lys 
        


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  GLP-Forward primer

<400>  71
aggggagtgc tgacacagag                                                   20


<210>  72
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  GLP-Reverse primer

<400>  72
gggatcttta ctggctgcat                                                   20


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  Beta-actin-Forward primer

<400>  73
ctcttccagc cttccttcct                                                   20


<210>  74
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  Beta-actin-Reverse primer

<400>  74
agcactgtgt tggcgtacag                                                   20


<210>  75
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  H1.0-Forward primer

<400>  75
ctcaagcaga ccaaaggggt                                                   20


<210>  76
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  H1.0-Reverse primer

<400>  76
ggcgtggcta ccttcttgat                                                   20


<210>  77
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  H1.1-Forward primer

<400>  77
aggcaacggg tgcatctaaa                                                   20


<210>  78
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  H1.1-Reverse primer

<400>  78
gatttccttg ttgccgcagg                                                   20


<210>  79
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  H1.2-Forward primer

<400>  79
caaagaaggc caaggttgcg                                                   20


<210>  80
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  H1.2-Reverse primer

<400>  80
cgccttctta ggcttgacaa c                                                 21


<210>  81
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  H1.3-Forward primer

<400>  81
agtggccaag agtgcgaaaa                                                   20


<210>  82
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  H1.3-Reverse primer

<400>  82
cttcggcttc cccgacttag                                                   20


<210>  83
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  H1.4-Forward primer

<400>  83
acgcttgcct tcaacatgtc c                                                 21


<210>  84
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  H1.4-Reverse primer

<400>  84
agtaatgagc tcggacaccg                                                   20


<210>  85
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  H1.5-Forward primer

<400>  85
ccggctaaga agaaggcaac                                                   20


<210>  86
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  H1.5-Reverse primer

<400>  86
gctccttaga agcagccaca                                                   20


<210>  87
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  G9A-Forward primer

<400>  87
tgctgaggct gatgtgagag                                                   20


<210>  88
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  G9A-Reverse primer

<400>  88
ggtcacacag gtggttgatg                                                   20


<210>  89
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  Lys is dimethylated

<400>  89

Cys Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val 
1               5                   10                  15      


Lys Lys Pro Lys 
            20  


<210>  90
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  Keyhole Limpet Hemocyanin (KLH) conjugated to a H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Cys is conjugated to Keyhole Limpet Hemocyanin

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Lys is dimethylated

<400>  90

Cys Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val 
1               5                   10                  15      


Lys Pro Lys 
            


<210>  91
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  biotinylated H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Cys is biotinylated

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Lys is dimethylated

<400>  91

Cys Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val 
1               5                   10                  15      


Lys Pro Lys 
            


<210>  92
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  bovine serum albumin (BSA)-conjugated H1.0K180me2 peptide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Cys is conjugated to bovine serum albumin (BSA)

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Lys is dimethylated

<400>  92

Cys Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val 
1               5                   10                  15      


Lys Pro Lys 
            


<210>  93
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  dimethylated K180me2 peptide


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Lys is methylated

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Lys is dimethylated

<400>  93

Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10                  15      


Pro Lys 
        


<210>  94
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  dimethylated K180me2 peptide


<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  Lys is trimethylated

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Lys is methylated

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Lys is dimethylated

<400>  94

Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10                  15      


Pro Lys 
        


<210>  95
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  dimethylated K180me2 peptide


<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Lys is methylated

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Lys is trimethylated

<400>  95

Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys Pro Val Lys 
1               5                   10                  15      


Pro Lys 
    


<210>  96
<211>  194
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  methylated H1.0 protein


<220>
<221>  misc_feature
<222>  (121)..(121)
<223>  Lys is dimethylated

<220>
<221>  misc_feature
<222>  (169)..(169)
<223>  Lys is dimethylated

<220>
<221>  misc_feature
<222>  (172)..(172)
<223>  Lys is methylated

<220>
<221>  misc_feature
<222>  (175)..(175)
<223>  Lys is dimethylated

<220>
<221>  misc_feature
<222>  (180)..(180)
<223>  Lys is dimethylated

<400>  96

Met Thr Glu Asn Ser Thr Ser Ala Pro Ala Ala Lys Pro Lys Arg Ala 
1               5                   10                  15      


Lys Ala Ser Lys Lys Ser Thr Asp His Pro Lys Tyr Ser Asp Met Ile 
            20                  25                  30          


Val Ala Ala Ile Gln Ala Glu Lys Asn Arg Ala Gly Ser Ser Arg Gln 
        35                  40                  45              


Ser Ile Gln Lys Tyr Ile Lys Ser His Tyr Lys Val Gly Glu Asn Ala 
    50                  55                  60                  


Asp Ser Gln Ile Lys Leu Ser Ile Lys Arg Leu Val Thr Thr Gly Val 
65                  70                  75                  80  


Leu Lys Gln Thr Lys Gly Val Gly Ala Ser Gly Ser Phe Arg Leu Ala 
                85                  90                  95      


Lys Ser Asp Glu Pro Lys Lys Ser Val Ala Phe Lys Lys Thr Lys Lys 
            100                 105                 110         


Glu Ile Lys Lys Val Ala Thr Pro Lys Lys Ala Ser Lys Pro Lys Lys 
        115                 120                 125             


Ala Ala Ser Lys Ala Pro Thr Lys Lys Pro Lys Ala Thr Pro Val Lys 
    130                 135                 140                 


Lys Ala Lys Lys Lys Leu Ala Ala Thr Pro Lys Lys Ala Lys Lys Pro 
145                 150                 155                 160 


Lys Thr Val Lys Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala 
                165                 170                 175     


Lys Pro Val Lys Pro Lys Ala Lys Ser Ser Ala Lys Arg Ala Gly Lys 
            180                 185                 190         


Lys Lys 
        


<210>  97
<211>  194
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  methylated H1.0 protein


<220>
<221>  misc_feature
<222>  (111)..(111)
<223>  Lys is dimethylated

<220>
<221>  misc_feature
<222>  (112)..(112)
<223>  Lys is trimethylated

<220>
<221>  misc_feature
<222>  (115)..(115)
<223>  Lys is dimethylated

<220>
<221>  misc_feature
<222>  (116)..(116)
<223>  Lys is trimethylated

<220>
<221>  misc_feature
<222>  (121)..(121)
<223>  Lys is trimethylated

<220>
<221>  misc_feature
<222>  (122)..(122)
<223>  Lys is dimethylated

<220>
<221>  misc_feature
<222>  (125)..(125)
<223>  Lys is methylated

<220>
<221>  misc_feature
<222>  (127)..(127)
<223>  Lys is dimethylated

<220>
<221>  misc_feature
<222>  (128)..(128)
<223>  Lys is trimethylated

<220>
<221>  misc_feature
<222>  (137)..(137)
<223>  Lys is trimethylated

<220>
<221>  misc_feature
<222>  (139)..(139)
<223>  Lys is dimethylated

<220>
<221>  misc_feature
<222>  (144)..(144)
<223>  Lys is methylated

<220>
<221>  misc_feature
<222>  (145)..(145)
<223>  Lys is trimethylated

<220>
<221>  misc_feature
<222>  (147)..(147)
<223>  Lys is dimethylated

<220>
<221>  misc_feature
<222>  (148)..(148)
<223>  Lys is trimethylated

<220>
<221>  misc_feature
<222>  (149)..(149)
<223>  Lys is methylated

<220>
<221>  misc_feature
<222>  (155)..(156)
<223>  Lys is trimethylated

<220>
<221>  misc_feature
<222>  (158)..(158)
<223>  Lys is dimethylated

<220>
<221>  misc_feature
<222>  (159)..(159)
<223>  Lys is methylated

<220>
<221>  misc_feature
<222>  (161)..(161)
<223>  Lys is trimethylated

<220>
<221>  misc_feature
<222>  (164)..(164)
<223>  Lys is trimethylated

<220>
<221>  misc_feature
<222>  (166)..(166)
<223>  Lys is dimethylated

<220>
<221>  misc_feature
<222>  (169)..(169)
<223>  Lys is trimethylated

<220>
<221>  misc_feature
<222>  (172)..(172)
<223>  Lys is methylated

<220>
<221>  misc_feature
<222>  (174)..(175)
<223>  Lys is trimethylated

<220>
<221>  misc_feature
<222>  (177)..(177)
<223>  Lys is dimethylated

<220>
<221>  misc_feature
<222>  (180)..(180)
<223>  Lys is methylated

<220>
<221>  misc_feature
<222>  (182)..(182)
<223>  Lys is methylated

<220>
<221>  misc_feature
<222>  (184)..(184)
<223>  Lys is trimethylated

<220>
<221>  misc_feature
<222>  (188)..(188)
<223>  Lys is dimethylated

<220>
<221>  misc_feature
<222>  (192)..(192)
<223>  Lys is dimethylated

<400>  97

Met Thr Glu Asn Ser Thr Ser Ala Pro Ala Ala Lys Pro Lys Arg Ala 
1               5                   10                  15      


Lys Ala Ser Lys Lys Ser Thr Asp His Pro Lys Tyr Ser Asp Met Ile 
            20                  25                  30          


Val Ala Ala Ile Gln Ala Glu Lys Asn Arg Ala Gly Ser Ser Arg Gln 
        35                  40                  45              


Ser Ile Gln Lys Tyr Ile Lys Ser His Tyr Lys Val Gly Glu Asn Ala 
    50                  55                  60                  


Asp Ser Gln Ile Lys Leu Ser Ile Lys Arg Leu Val Thr Thr Gly Val 
65                  70                  75                  80  


Leu Lys Gln Thr Lys Gly Val Gly Ala Ser Gly Ser Phe Arg Leu Ala 
                85                  90                  95      


Lys Ser Asp Glu Pro Lys Lys Ser Val Ala Phe Lys Lys Thr Lys Lys 
            100                 105                 110         


Glu Ile Lys Lys Val Ala Thr Pro Lys Lys Ala Ser Lys Pro Lys Lys 
        115                 120                 125             


Ala Ala Ser Lys Ala Pro Thr Lys Lys Pro Lys Ala Thr Pro Val Lys 
    130                 135                 140                 


Lys Ala Lys Lys Lys Leu Ala Ala Thr Pro Lys Lys Ala Lys Lys Pro 
145                 150                 155                 160 


Lys Thr Val Lys Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala 
                165                 170                 175     


Lys Pro Val Lys Pro Lys Ala Lys Ser Ser Ala Lys Arg Ala Gly Lys 
            180                 185                 190         


Lys Lys 
        


