                         SEQUENCE LISTING

<110>  Mayo Foundation for Medical Education and Research
 
<120>  MATERIALS AND METHODS FOR TREATING CANCER

<130>  07039-1564WO1

<150>  US 62/335785
<151>  2016-05-13

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  30
<212>  PRT
<213>  artificial

<220>
<223>  HA-tagged FOXO1-derived IQGAP1-binding peptide

<400>  1

Asn Asp Asp Phe Asp Asn Trp Ser Thr Phe Arg Pro Arg Thr Ser Ser 
1               5                   10                  15      


Asn Ala Ser Thr Ile Ser Gly Arg Leu Ser Pro Ile Met Thr 
            20                  25                  30  


<210>  2
<211>  30
<212>  PRT
<213>  artificial

<220>
<223>  phosphorylation-mimicking FOXO1-derived peptide

<400>  2

Asn Asp Asp Phe Asp Asn Trp Ser Thr Phe Arg Pro Arg Thr Ser Glu 
1               5                   10                  15      


Asn Ala Ser Thr Ile Ser Gly Arg Leu Ser Pro Ile Met Thr 
            20                  25                  30  


<210>  3
<211>  30
<212>  PRT
<213>  artificial

<220>
<223>  phosphorylation-mimicking FOXO1-derived peptide

<400>  3

Asn Asp Asp Phe Asp Asn Trp Ser Thr Phe Arg Pro Arg Thr Ser Asp 
1               5                   10                  15      


Asn Ala Ser Thr Ile Ser Gly Arg Leu Ser Pro Ile Met Thr 
            20                  25                  30  


<210>  4
<211>  47
<212>  PRT
<213>  artificial

<220>
<223>  phosphorylation-mimicking FOXO1-derived peptide

<400>  4

Arg Arg Arg Arg Arg Arg Arg Arg Tyr Pro Tyr Asp Val Pro Asp Tyr 
1               5                   10                  15      


Ala Asn Asp Asp Phe Asp Asn Trp Ser Thr Phe Arg Pro Arg Thr Ser 
            20                  25                  30          


Glu Asn Ala Ser Thr Ile Ser Gly Arg Leu Ser Pro Ile Met Thr 
        35                  40                  45          


<210>  5
<211>  47
<212>  PRT
<213>  artificial

<220>
<223>  phosphorylation-mimicking FOXO1-derived peptide

<400>  5

Arg Arg Arg Arg Arg Arg Arg Arg Tyr Pro Tyr Asp Val Pro Asp Tyr 
1               5                   10                  15      


Ala Asn Asp Asp Phe Asp Asn Trp Ser Thr Phe Arg Pro Arg Thr Ser 
            20                  25                  30          


Asp Asn Ala Ser Thr Ile Ser Gly Arg Leu Ser Pro Ile Met Thr 
        35                  40                  45          


<210>  6
<211>  10
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(10)
<223>  FOXO1 nuclear export signal motif

<400>  6

Met Glu Asn Leu Leu Asp Asn Leu Asn Leu 
1               5                   10  


<210>  7
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  mutated FOXO1 nuclear export signal motif

<400>  7

Ala Glu Asn Ala Leu Asp Asn Ala Asn Ala 
1               5                   10  


<210>  8
<211>  58
<212>  DNA
<213>  artificial

<220>
<223>  shRNA for human IQGAP1

<400>  8
ccgggcccac attgtgcctt tatttctcga gaaataaagg cacaatgtgg gctttttg         58


<210>  9
<211>  58
<212>  DNA
<213>  artificial

<220>
<223>  shRNA for human IQGAP1

<400>  9
ccggcctcag attcaagacc tatatctcga gatataggtc ttgaatctga ggtttttg         58


<210>  10
<211>  58
<212>  DNA
<213>  artificial

<220>
<223>  shRNA for human FOXO-1

<400>  10
ccgggccgga gtttagccag tccaactcga gttggactgg ctaaactccg gctttttg         58


<210>  11
<211>  57
<212>  DNA
<213>  artificial

<220>
<223>  shRNA for human FOXO-1

<400>  11
ccggatctac gagtggatgg tcaactcgag ttgaccatcc actcgtagat ctttttg          57


<210>  12
<211>  58
<212>  DNA
<213>  artificial

<220>
<223>  shRNA for human FOXO-1

<400>  12
ccggcagacc ctcaaactga cacaactcga gttgtgtcag tttgagggtc tgtttttg         58


<210>  13
<211>  58
<212>  DNA
<213>  artificial

<220>
<223>  shRNA for human FOXO-1

<400>  13
ccgggtcact gcatagtcga ttcatctcga gatgaatcga ctatgcagtg actttttg         58


<210>  14
<211>  59
<212>  DNA
<213>  artificial

<220>
<223>  shRNA for human PKM2

<400>  14
ccgggcccga ggcttcttca agaagctcga gcttcttgaa gaagcctcgg gcttttttg        59


<210>  15
<211>  59
<212>  DNA
<213>  artificial

<220>
<223>  shRNA for human PKM2

<400>  15
ccgggttcgg aggtttgatg aaatcctcga ggatttcatc aaacctccga acttttttg        59


<210>  16
<211>  59
<212>  DNA
<213>  artificial

<220>
<223>  shRNA for human PKM2

<400>  16
ccggctttcc tgtgtgtact ctgtcctcga ggacagagta cacacaggaa agttttttg        59


<210>  17
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  synthetic primer

<400>  17
gctgtgctta tgggcttctc                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  synthetic primer

<400>  18
cacatacatg ggcacaaagc                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  synthetic primer

<400>  19
tggagtggaa tgaatgttgc                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  synthetic primer

<400>  20
atagcccagg atgtgtagcc                                                   20


<210>  21
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  synthetic primer

<400>  21
attatttgag gaactccgcc gcct                                              24


<210>  22
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  synthetic primer

<400>  22
attccgggtc acagcaatga tgg                                               23


<210>  23
<211>  27
<212>  PRT
<213>  artificial

<220>
<223>  FOXO1- interacting IQGAP1 peptide

<400>  23

Lys Thr Val Leu Glu Leu Met Asn Pro Glu Ala Gln Leu Pro Gln Val 
1               5                   10                  15      


Tyr Pro Phe Ala Ala Asp Leu Tyr Gln Lys Glu 
            20                  25          


<210>  24
<211>  20
<212>  PRT
<213>  artificial

<220>
<223>  FOXO1- interacting IQGAP1 peptide

<400>  24

Arg Ile Leu Ala Ile Gly Leu Ile Asn Glu Ala Leu Asp Glu Gly Asp 
1               5                   10                  15      


Ala Gln Lys Thr 
            20  


<210>  25
<211>  21
<212>  PRT
<213>  artificial

<220>
<223>  FOXO1- interacting IQGAP1 peptide

<400>  25

Lys Leu Glu Gly Val Leu Ala Glu Val Ala Gln His Tyr Gln Asp Thr 
1               5                   10                  15      


Ile Leu Ile Arg Ala 
            20      


<210>  26
<211>  17
<212>  PRT
<213>  artificial

<220>
<223>  FOXO1- interacting IQGAP1 peptide

<400>  26

Arg Ser Asn Gln Gln Leu Glu Asn Asp Leu Asn Leu Met Asp Ile Lys 
1               5                   10                  15      


Ile 
    


<210>  27
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  FOXO1- interacting IQGAP1 peptide

<400>  27

Lys Leu Ile Phe Gln Met Pro Gln Asn Lys Ser 
1               5                   10      


<210>  28
<211>  27
<212>  PRT
<213>  artificial

<220>
<223>  FOXO1- interacting IQGAP1 peptide

<400>  28

Arg Phe Gln Pro Gly Glu Thr Leu Thr Glu Ile Leu Glu Thr Pro Ala 
1               5                   10                  15      


Thr Ser Glu Gln Glu Ala Glu His Gln Arg Ala 
            20                  25          


<210>  29
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  FOXO1 AKT phosphorylation site consensus motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  X is S or T

<400>  29

Arg Xaa Arg Xaa Xaa Xaa 
1               5       


<210>  30
<211>  13
<212>  PRT
<213>  artificial

<220>
<223>  FOXO1 AKT phosphorylation site

<400>  30

Thr Phe Arg Pro Arg Thr Ser Ser Asn Ala Ser Thr Ile 
1               5                   10              


<210>  31
<211>  13
<212>  PRT
<213>  artificial

<220>
<223>  FOXO1 AKT phosphorylation site

<400>  31

Asp Phe Arg Ser Arg Thr Asn Ser Asn Ala Ser Thr Val 
1               5                   10              


<210>  32
<211>  13
<212>  PRT
<213>  artificial

<220>
<223>  FOXO1 AKT phosphorylation site

<400>  32

Thr Arg Phe Pro Arg Ser Ser Ser Asn Ala Ser Ser Val 
1               5                   10              


