                         LISTADO DE SECUENCIAS

<110>  Abengoa Bioenerga Nuevas Tecnologas, S.A.
 
<120>  Clula hospedadora Myceliophthora thermophila con actividad 
       celuloltica mejorada y composiciones enzimticas obtenidas por
       la misma.

<130>  ES1861.52

<150>  P201630658
<151>  2016-05-20

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  1223
<212>  DNA
<213>  Myceliophthora thermophila

<400>  1
atgcgcgtct ctagtttggt cgcggccctt gctaccggtg gtcttgtcgc cgccacgcct       60

aagcccaagg ggtcgtcgcc ccctggggcc gtggacgcga accctttcaa gggcaagacg      120

cagttcgtca acccggcatg ggcggccaag ctggaacaga ccaaaaaggc gttcctggcc      180

aggaacgaca ccgtcaatgc cgccaagacg gagaaggtcc agcagaccag ctcgttcgtc      240

tgggtctcga ggatcgccga gctctccaac atcgacgacg ccatcgcggc tgcccgcaag      300

gcgcagaaga agacgggcag gaggcagatc gtcggcctgg tgctctacaa ccttccggac      360

cgcgactgca gcgcgggcga gagcgcgggc gagctcagca gcgacaagaa cgggctcgag      420

atctacaaga ctgagttcgt caagcccttc gccgacaagg tggcggccgc aaaggacctc      480

gacttcgcca tcgtcctgga gcccgactcg ctggccaacc tggtcaccaa cctgggcatc      540

gagttctgcg ccaacgccgc ccccgtctac cgcgagggca tcgcctatgc catctccagc      600

cttcagcagc caaacgtgca cttgtacatc gatgctgccc acggcggctg gctcggctgg      660

gacgacaacc tgccgctggc cgccaaggag tttgccgagg tggtcaagct tgccggcgag      720

ggcaagaaga tccgcggctt cgtcaccaac gtgtccaact acaacccctt ccacgccgtc      780

gtgcgcgaga actttaccga gtggagcaac tcgtgggacg agtctcacta cgcctcctcg      840

ctcacaccgt tcctcgagaa agaggggctg ccggcacgct tcatcgtcga ccagggtcgc      900

gttgccctcc cgggagcccg caaggagtgg tgagtttcga ccagattgac cctcgaccca      960

tgcgaccgag attgctgacg attgaattgc gtgtcccgtc ccccaggggt gaatggtgca     1020

acgtggcacc cgccggattt ggccccgcgc ccacgaccag ggtcaacaac accgtcgtcg     1080

atgctctcgt ctgggtcaag cctggcggcg agagcgacgg cgagtgtggc ttggctggcg     1140

cccccaaggc cggccagtgg ttcgacgagt acgcccagat gctggtcgag aatgcccacc     1200

cgtctgtcgt ccacaagtgg tag                                             1223


<210>  2
<211>  381
<212>  PRT
<213>  Myceliophthora thermophila

<400>  2

Met Arg Val Ser Ser Leu Val Ala Ala Leu Ala Thr Gly Gly Leu Val 
1               5                   10                  15      


Ala Ala Thr Pro Lys Pro Lys Gly Ser Ser Pro Pro Gly Ala Val Asp 
            20                  25                  30          


Ala Asn Pro Phe Lys Gly Lys Thr Gln Phe Val Asn Pro Ala Trp Ala 
        35                  40                  45              


Ala Lys Leu Glu Gln Thr Lys Lys Ala Phe Leu Ala Arg Asn Asp Thr 
    50                  55                  60                  


Val Asn Ala Ala Lys Thr Glu Lys Val Gln Gln Thr Ser Ser Phe Val 
65                  70                  75                  80  


Trp Val Ser Arg Ile Ala Glu Leu Ser Asn Ile Asp Asp Ala Ile Ala 
                85                  90                  95      


Ala Ala Arg Lys Ala Gln Lys Lys Thr Gly Arg Arg Gln Ile Val Gly 
            100                 105                 110         


Leu Val Leu Tyr Asn Leu Pro Asp Arg Asp Cys Ser Ala Gly Glu Ser 
        115                 120                 125             


Ala Gly Glu Leu Ser Ser Asp Lys Asn Gly Leu Glu Ile Tyr Lys Thr 
    130                 135                 140                 


Glu Phe Val Lys Pro Phe Ala Asp Lys Val Ala Ala Ala Lys Asp Leu 
145                 150                 155                 160 


Asp Phe Ala Ile Val Leu Glu Pro Asp Ser Leu Ala Asn Leu Val Thr 
                165                 170                 175     


Asn Leu Gly Ile Glu Phe Cys Ala Asn Ala Ala Pro Val Tyr Arg Glu 
            180                 185                 190         


Gly Ile Ala Tyr Ala Ile Ser Ser Leu Gln Gln Pro Asn Val His Leu 
        195                 200                 205             


Tyr Ile Asp Ala Ala His Gly Gly Trp Leu Gly Trp Asp Asp Asn Leu 
    210                 215                 220                 


Pro Leu Ala Ala Lys Glu Phe Ala Glu Val Val Lys Leu Ala Gly Glu 
225                 230                 235                 240 


Gly Lys Lys Ile Arg Gly Phe Val Thr Asn Val Ser Asn Tyr Asn Pro 
                245                 250                 255     


Phe His Ala Val Val Arg Glu Asn Phe Thr Glu Trp Ser Asn Ser Trp 
            260                 265                 270         


Asp Glu Ser His Tyr Ala Ser Ser Leu Thr Pro Phe Leu Glu Lys Glu 
        275                 280                 285             


Gly Leu Pro Ala Arg Phe Ile Val Asp Gln Gly Arg Val Ala Leu Pro 
    290                 295                 300                 


Gly Ala Arg Lys Glu Trp Gly Glu Trp Cys Asn Val Ala Pro Ala Gly 
305                 310                 315                 320 


Phe Gly Pro Ala Pro Thr Thr Arg Val Asn Asn Thr Val Val Asp Ala 
                325                 330                 335     


Leu Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Glu Cys Gly Leu 
            340                 345                 350         


Ala Gly Ala Pro Lys Ala Gly Gln Trp Phe Asp Glu Tyr Ala Gln Met 
        355                 360                 365             


Leu Val Glu Asn Ala His Pro Ser Val Val His Lys Trp 
    370                 375                 380     


<210>  3
<211>  749
<212>  DNA
<213>  Myceliophthora thermophila

<400>  3
atgctgacaa caaccttcgc cctcctgacg gccgctctcg gcgtcagcgc ccattatacc       60

ctccccaggg tcgggaccgg ttccgactgg cagcacgtgc ggcgggctga caactggcaa      120

aacaacggct tcgtcggcga cgtcaactcg gagcagatca ggtgcttcca ggcgacccct      180

gccggcgccc aagacgtcta cactgttcag gcgggatcga ccgtgaccta ccacgccaac      240

cccagtatct accaccccgg ccccatgcag ttctacctgg cccgcgttcc ggacggacag      300

gacgtcaagt cgtggaccgg cgagggtgcc gtgtggttca aggtgtacga ggagcagcct      360

caatttggcg cccagctgac ctggcctagc aacggtgcgt tgatcatttt ccttcttctt      420

ccttctttct tccgttgcat atgctaactg ttctcttgct tgcaggcaag agctcgttcg      480

aggttcctat ccccagctgc attcgggcgg gcaactacct cctccgcgct gagcacatcg      540

ccctgcacgt tgcccaaagc cagggcggcg cccagttcta catctcgtgc gcccagctcc      600

aggtcactgg tggcggcagc accgagcctt ctcagaaggt ttccttcccg ggtgcctaca      660

agtccaccga ccccggcatt cttatcaaca tcaactaccc cgtccctacc tcgtaccaga      720

atccgggtcc ggctgtcttc cgttgctaa                                        749


<210>  4
<211>  225
<212>  PRT
<213>  Myceliophthora thermophila

<400>  4

Met Leu Thr Thr Thr Phe Ala Leu Leu Thr Ala Ala Leu Gly Val Ser 
1               5                   10                  15      


Ala His Tyr Thr Leu Pro Arg Val Gly Thr Gly Ser Asp Trp Gln His 
            20                  25                  30          


Val Arg Arg Ala Asp Asn Trp Gln Asn Asn Gly Phe Val Gly Asp Val 
        35                  40                  45              


Asn Ser Glu Gln Ile Arg Cys Phe Gln Ala Thr Pro Ala Gly Ala Gln 
    50                  55                  60                  


Asp Val Tyr Thr Val Gln Ala Gly Ser Thr Val Thr Tyr His Ala Asn 
65                  70                  75                  80  


Pro Ser Ile Tyr His Pro Gly Pro Met Gln Phe Tyr Leu Ala Arg Val 
                85                  90                  95      


Pro Asp Gly Gln Asp Val Lys Ser Trp Thr Gly Glu Gly Ala Val Trp 
            100                 105                 110         


Phe Lys Val Tyr Glu Glu Gln Pro Gln Phe Gly Ala Gln Leu Thr Trp 
        115                 120                 125             


Pro Ser Asn Gly Lys Ser Ser Phe Glu Val Pro Ile Pro Ser Cys Ile 
    130                 135                 140                 


Arg Ala Gly Asn Tyr Leu Leu Arg Ala Glu His Ile Ala Leu His Val 
145                 150                 155                 160 


Ala Gln Ser Gln Gly Gly Ala Gln Phe Tyr Ile Ser Cys Ala Gln Leu 
                165                 170                 175     


Gln Val Thr Gly Gly Gly Ser Thr Glu Pro Ser Gln Lys Val Ser Phe 
            180                 185                 190         


Pro Gly Ala Tyr Lys Ser Thr Asp Pro Gly Ile Leu Ile Asn Ile Asn 
        195                 200                 205             


Tyr Pro Val Pro Thr Ser Tyr Gln Asn Pro Gly Pro Ala Val Phe Arg 
    210                 215                 220                 


Cys 
225 


<210>  5
<211>  3310
<212>  DNA
<213>  Myceliophthora thermophila


<220>
<221>  promoter
<222>  (1)..(740)

<220>
<221>  terminator
<222>  (2359)..(3310)

<400>  5
aggttttgtg cggtatggag ctaataatat tgaacggatc tctggtccgt cctaaatcgt       60

tgaaacgcta ggcccagaag gacctgctcg acttggcgaa cggagatttc caggacgaaa      120

gtcggaacat ctccatccgc ggccaacctg aacacttttg ttcgtttccg gaccatcgac      180

ccacgaaaac agtgcggttg ctggcacagt cagtactcac aatggcgatg gtccagcccg      240

ttcccgcccg atgcccactt gcagcgcaac tctccttcgt tcggcggccc ggcggtgtct      300

ggcctattag tacgattttg gataccggct tggtcgccgc cgcggttttt cttggccgat      360

acgggaatct cggtggtccc aactccacct gggcacgctc tggtgccaac atggaacttc      420

gggatgccgc tccgggcaca gtcaagcgct ttaaaatgcg accttactcc acaagaatcg      480

aggcgtaacc cggaattagg gacgcctgga cggcgcaacc cctggaccga agggcctcgc      540

taaccgggtt cctggagccg catgcgcggc tgcccgcttg cccgctcttg aggtgacact      600

tcttttcaac gagcgatggc cgggcaggaa aatgatgtat aagaagcgag ccgattccga      660

cggactcgac ctctctctcg cctcttgccc tgtgtccgcg agctaattac agcagtcctt      720

ctcgacttga aacgccccaa atgaagtcct ccatcctcgc cagcgtcttc gccacgggcg      780

ccgtggctca aagtggtccg tggcagcaat gtggtggcat cggatggcaa ggatcgaccg      840

actgtgtgtc gggctaccac tgcgtctacc agaacgattg gtacagccag tgcgtgcctg      900

gcgcggcgtc gacaacgctg cagacatcga ccacgtccag gcccaccgcc accagcaccg      960

cccctccgtc gtccaccacc tcgcctagca agggcaagct gaagtggctc ggcagcaacg     1020

agtcgggcgc cgagttcggg gagggcaatt accccggcct ctggggcaag cacttcatct     1080

tcccgtcgac ttcggcgatt caggtacggc caataataat atattattat agcaggcagg     1140

agggagcagg agaagaaggg aggggcaggt ggcccacaat cggaagaaga ccgggaggca     1200

ctgaccgttg attcctttgt gtaatagacg ctcatcaatg atggatacaa catcttccgg     1260

atcgacttct cgatggagcg tctggtgccc aaccagttga cgtcgtcctt cgaccagggt     1320

tacctccgca acctgaccga ggtggtcaac ttcgtgacga acgcgggcaa gtacgccgtc     1380

ctggacccgc acaactacgg ccggtactac ggcaacatca tcacggacac gaacgcgttc     1440

cggaccttct ggaccaacct ggccaagcag ttcgcctcca actcgctcgt catcttcgac     1500

accaacaacg agtacaacac gatggaccag accctggtgc tcaacctcaa ccaggccgcc     1560

atcgacggca tccgggccgc cggcgcgacc tcgcagtaca tcttcgtcga gggcaacgcg     1620

tggagcgggg cctggagctg gaacacgacc aacaccaaca tggccgccct gacggacccg     1680

cagaacaaga tcgtgtacga gatgcaccag tacctcgact cggacagctc gggcacccac     1740

gccgagtgcg tcagcagcac catcggcgcc cagcgcgtcg tcggagccac ccagtggctc     1800

cgcgccaacg gcaagctcgg cgtcctcggc gagttcgccg gcggcgccaa cgccgtctgc     1860

cagcaggccg tcaccggcct cctcgaccac ctccaggaca acagcgacgt ctggctgggt     1920

gccctctggt gggccgccgg tccctggtgg ggcgactaca tgtactcgtt cggtaagttt     1980

ctcccttgtt cttggtttcc cccccaaata agggagtcag gcaacatgcc caagatcggc     2040

tcggcttcgc ttcaaggcgt tcgttgtaca cactgaagag ttccaactcc caacctgttc     2100

gtgtcctccg atcagcttca acgggggtga agggggaagg gatttgggag tgaggtggag     2160

gtcaaaagga ggggtattat ccccagacct ccacaaacgg ccctgagcca acagcctctg     2220

gggtcaaaat gggcgccaac catacggtca ttcactcagg acacctgcta acgcgcctct     2280

ttttttttgt ttccagagcc tccttcgggc accggctatg tcaactacaa ctcgatcttg     2340

aagaagtact tgccgtaagg ggcgtgcagc aaggtcgagc gagcattatt cggggccatc     2400

tgcttgtgtc ggcaggcatc acgtcaaccc atcgaattgg acagcggaat gctccgagac     2460

gccctacact aagtctggtg atgatcccgg acgctgccgc gcccggatac catgatttca     2520

tgtacatatt ggttctttgc tttcttaccg ggggggctct gcagcgttgc tgagcgattc     2580

gtttccaagt atatactttg tctggaattg aattttgggt gacattgacc caatcaacca     2640

gctcggcgtg ctcacctccc gttacccccc ctcttctccc cctgctcggc ttggctttcc     2700

tctccggtgt ggagcacggc cacggcggtc tcaatccata caggaagata agatcgatgg     2760

tttcctatgg tatacactac ttgggaataa actaatccgt acgctaacta atggacggat     2820

tatcctaagg gtcaccggct caccgttgga tataacactt agggtatggg agagctgata     2880

gaaatattgt actgtacaat ccaaagtaca gatagcacac gaagtatggt agttggtccc     2940

gcccagtccg gaccaacaat agaacaatac acgcacgagc agccgtccac tacgcagcag     3000

tgtgcgttcc tgaggacctg caggaagagg gggggggggg ggttgccaag acgcccgggg     3060

ttcaaagaaa gtcccgggcc gccgatgaga tgagacggac gccggcccaa ggagaggccg     3120

gtggtcgatc ctgcaaatgc cagcaaaaaa atccatacca taatccagtc aactttcgtc     3180

acactcctgt gaaacgagct ggagggactg ctggaaaggt tttgcaggtt aatcactgta     3240

tgtggagcat gccgtaccta ccatgcttcg ttagatagag ttccagttga acatacaaag     3300

ttctgccccg                                                            3310


<210>  6
<211>  389
<212>  PRT
<213>  Myceliophthora thermophila

<400>  6

Met Lys Ser Ser Ile Leu Ala Ser Val Phe Ala Thr Gly Ala Val Ala 
1               5                   10                  15      


Gln Ser Gly Pro Trp Gln Gln Cys Gly Gly Ile Gly Trp Gln Gly Ser 
            20                  25                  30          


Thr Asp Cys Val Ser Gly Tyr His Cys Val Tyr Gln Asn Asp Trp Tyr 
        35                  40                  45              


Ser Gln Cys Val Pro Gly Ala Ala Ser Thr Thr Leu Gln Thr Ser Thr 
    50                  55                  60                  


Thr Ser Arg Pro Thr Ala Thr Ser Thr Ala Pro Pro Ser Ser Thr Thr 
65                  70                  75                  80  


Ser Pro Ser Lys Gly Lys Leu Lys Trp Leu Gly Ser Asn Glu Ser Gly 
                85                  90                  95      


Ala Glu Phe Gly Glu Gly Asn Tyr Pro Gly Leu Trp Gly Lys His Phe 
            100                 105                 110         


Ile Phe Pro Ser Thr Ser Ala Ile Gln Thr Leu Ile Asn Asp Gly Tyr 
        115                 120                 125             


Asn Ile Phe Arg Ile Asp Phe Ser Met Glu Arg Leu Val Pro Asn Gln 
    130                 135                 140                 


Leu Thr Ser Ser Phe Asp Glu Gly Tyr Leu Arg Asn Leu Thr Glu Val 
145                 150                 155                 160 


Val Asn Phe Val Thr Asn Ala Gly Lys Tyr Ala Val Leu Asp Pro His 
                165                 170                 175     


Asn Tyr Gly Arg Tyr Tyr Gly Asn Val Ile Thr Asp Thr Asn Ala Phe 
            180                 185                 190         


Arg Thr Phe Trp Thr Asn Leu Ala Lys Gln Phe Ala Ser Asn Ser Leu 
        195                 200                 205             


Val Ile Phe Asp Thr Asn Asn Glu Tyr Asn Thr Met Asp Gln Thr Leu 
    210                 215                 220                 


Val Leu Asn Leu Asn Gln Ala Ala Ile Asp Gly Ile Arg Ala Ala Gly 
225                 230                 235                 240 


Ala Thr Ser Gln Tyr Ile Phe Val Glu Gly Asn Ala Trp Ser Gly Ala 
                245                 250                 255     


Trp Ser Trp Asn Thr Thr Asn Thr Asn Met Ala Ala Leu Thr Asp Pro 
            260                 265                 270         


Gln Asn Lys Ile Val Tyr Glu Met His Gln Tyr Leu Asp Ser Asp Ser 
        275                 280                 285             


Ser Gly Thr His Ala Glu Cys Val Ser Ser Asn Ile Gly Ala Gln Arg 
    290                 295                 300                 


Val Val Gly Ala Thr Gln Trp Leu Arg Ala Asn Gly Lys Leu Gly Val 
305                 310                 315                 320 


Leu Gly Glu Phe Ala Gly Gly Ala Asn Ala Val Cys Gln Gln Ala Val 
                325                 330                 335     


Thr Gly Leu Leu Asp His Leu Gln Asp Asn Ser Asp Val Trp Leu Gly 
            340                 345                 350         


Ala Leu Trp Trp Ala Ala Gly Pro Trp Trp Gly Asp Tyr Met Tyr Ser 
        355                 360                 365             


Phe Glu Pro Pro Ser Gly Thr Gly Tyr Val Asn Tyr Asn Ser Ile Leu 
    370                 375                 380                 


Lys Lys Tyr Leu Pro 
385                 


<210>  7
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador directo - Oligonucletido 1

<400>  7
accgagctcg tagcactcgc tgtgtatcct c                                      31


<210>  8
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador inverso - Oligonucletido 2

<400>  8
cctggatccc ttatacccag gacattcaca gttc                                   34


<210>  9
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador directo - Oligonucletido 3

<400>  9
accgaattca tcaaatggat aggtcggtaa tg                                     32


<210>  10
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador inverso - Oligonucletido 4

<400>  10
cacctcgagc aaggaagtcg agtacgagtc c                                      31


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador directo - Oligonucletido 5

<400>  11
ggctcgagat ctacaagact g                                                 21


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador inverso - Oligonucletido 6

<400>  12
gtagttggac acgttggtga                                                   20


