                         SEQUENCE LISTING

<110>  Universidad Nacional Autonoma de Mexico
       Bravo de la Parra, Maria A.
       Soberon Chavez, Mario
 
<120>  BACILLUS THURINGIENSIS CYT1A MUTANTS

<130>  36446.0342P1

<150>  62/337,656
<151>  2016-05-17

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  747
<212>  DNA
<213>  Bacillus thuringiensis

<400>  1
atggaaaatt taaatcattg tccattagaa gatataaagg taaatccatg gaaaacccct       60

caatcaacag caagggttat tacattacgt gttgaggatc caaatgaaat caataatctt      120

ctttctatta acgaaattga taatccgaat tatatattgc aagcaattat gttagcaaat      180

gcatttcaaa atgcattagt tcccacttct acagattttg gtgatgccct acgctttagt      240

atgccaaaag gtttagaaat cgcaaacaca attacaccga tgggtgctgt agtgagttat      300

gttgatcaaa atgtaactca aacgaataac caagtaagtg ttatgattaa taaagtctta      360

gaagtgttaa aaactgtatt aggagttgca ttaagtggat ctgtaataga tcaattaact      420

gcagcagtta caaatacgtt tacaaattta aatactcaaa aaaatgaagc atggattttc      480

tggggcaagg aaactgctaa tcaaacaaat tacacataca atgtcctgtt tgcaatccaa      540

aatgcccaaa ctggtggcgt tatgtattgt gtaccagttg gttttgaaat taaagtatca      600

gcagtaaagg aacaagtttt atttttcaca attcaagatt ctgcgagcta caatgttaac      660

atccaatctt tgaaatttgc acaaccatta gttagctcaa gtcagtatcc aattgcagat      720

cttactagcg ctattaatgg aaccctc                                          747


<210>  2
<211>  249
<212>  PRT
<213>  Bacillus thuringiensis

<400>  2

Met Glu Asn Leu Asn His Cys Pro Leu Glu Asp Ile Lys Val Asn Pro 
1               5                   10                  15      


Trp Lys Thr Pro Gln Ser Thr Ala Arg Val Ile Thr Leu Arg Val Glu 
            20                  25                  30          


Asp Pro Asn Glu Ile Asn Asn Leu Leu Ser Ile Asn Glu Ile Asp Asn 
        35                  40                  45              


Pro Asn Tyr Ile Leu Gln Ala Ile Met Leu Ala Asn Ala Phe Gln Asn 
    50                  55                  60                  


Ala Leu Val Pro Thr Ser Thr Asp Phe Gly Asp Ala Leu Arg Phe Ser 
65                  70                  75                  80  


Met Pro Lys Gly Leu Glu Ile Ala Asn Thr Ile Thr Pro Met Gly Ala 
                85                  90                  95      


Val Val Ser Tyr Val Asp Gln Asn Val Thr Gln Thr Asn Asn Gln Val 
            100                 105                 110         


Ser Val Met Ile Asn Lys Val Leu Glu Val Leu Lys Thr Val Leu Gly 
        115                 120                 125             


Val Ala Leu Ser Gly Ser Val Ile Asp Gln Leu Thr Ala Ala Val Thr 
    130                 135                 140                 


Asn Thr Phe Thr Asn Leu Asn Thr Gln Lys Asn Glu Ala Trp Ile Phe 
145                 150                 155                 160 


Trp Gly Lys Glu Thr Ala Asn Gln Thr Asn Tyr Thr Tyr Asn Val Leu 
                165                 170                 175     


Phe Ala Ile Gln Asn Ala Gln Thr Gly Gly Val Met Tyr Cys Val Pro 
            180                 185                 190         


Val Gly Phe Glu Ile Lys Val Ser Ala Val Lys Glu Gln Val Leu Phe 
        195                 200                 205             


Phe Thr Ile Gln Asp Ser Ala Ser Tyr Asn Val Asn Ile Gln Ser Leu 
    210                 215                 220                 


Lys Phe Ala Gln Pro Leu Val Ser Ser Ser Gln Tyr Pro Ile Ala Asp 
225                 230                 235                 240 


Leu Thr Ser Ala Ile Asn Gly Thr Leu 
                245                 


<210>  3
<211>  747
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyt1Aa variant

<400>  3
atggaaaatt taaatcattg tccattagaa gatataaagg taaatccatg gaaaacccct       60

caatcaacag caagggttat tacattacgt gttgaggatc caaatgaaat caataatctt      120

ctttctatta acgaaattga taatccgaat tatatattgc aagcaattat gttatgtaat      180

gcatttcaaa atgcattagt tcccacttct acagattttg gtgatgccct acgctttagt      240

atgccaaaag gtttagaaat cgcaaacaca attacaccga tgggtgctgt agtgagttat      300

gttgatcaaa atgtaactca aacgaataac caagtaagtg ttatgattaa taaagtctta      360

gaagtgttaa aaactgtatt aggagttgca ttaagtggat ctgtaataga tcaattaact      420

gcagcagtta caaatacgtt tacaaattta aatactcaaa aaaatgaagc atggattttc      480

tggggcaagg aaactgctaa tcaaacaaat tacacataca atgtcctgtt tgcaatccaa      540

aatgcccaaa ctggtggcgt tatgtattgt gtaccagttg gttttgaaat taaagtatca      600

gcagtaaagg aacaagtttt atttttcaca attcaagatt ctgcgagcta caatgttaac      660

atccaatctt tgaaatttgc acaaccatta gttagctcaa gtcagtatcc aattgcagat      720

cttactagcg ctattaatgg aaccctc                                          747


<210>  4
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cyt1Aa variant

<400>  4

Met Glu Asn Leu Asn His Cys Pro Leu Glu Asp Ile Lys Val Asn Pro 
1               5                   10                  15      


Trp Lys Thr Pro Gln Ser Thr Ala Arg Val Ile Thr Leu Arg Val Glu 
            20                  25                  30          


Asp Pro Asn Glu Ile Asn Asn Leu Leu Ser Ile Asn Glu Ile Asp Asn 
        35                  40                  45              


Pro Asn Tyr Ile Leu Gln Ala Ile Met Leu Cys Asn Ala Phe Gln Asn 
    50                  55                  60                  


Ala Leu Val Pro Thr Ser Thr Asp Phe Gly Asp Ala Leu Arg Phe Ser 
65                  70                  75                  80  


Met Pro Lys Gly Leu Glu Ile Ala Asn Thr Ile Thr Pro Met Gly Ala 
                85                  90                  95      


Val Val Ser Tyr Val Asp Gln Asn Val Thr Gln Thr Asn Asn Gln Val 
            100                 105                 110         


Ser Val Met Ile Asn Lys Val Leu Glu Val Leu Lys Thr Val Leu Gly 
        115                 120                 125             


Val Ala Leu Ser Gly Ser Val Ile Asp Gln Leu Thr Ala Ala Val Thr 
    130                 135                 140                 


Asn Thr Phe Thr Asn Leu Asn Thr Gln Lys Asn Glu Ala Trp Ile Phe 
145                 150                 155                 160 


Trp Gly Lys Glu Thr Ala Asn Gln Thr Asn Tyr Thr Tyr Asn Val Leu 
                165                 170                 175     


Phe Ala Ile Gln Asn Ala Gln Thr Gly Gly Val Met Tyr Cys Val Pro 
            180                 185                 190         


Val Gly Phe Glu Ile Lys Val Ser Ala Val Lys Glu Gln Val Leu Phe 
        195                 200                 205             


Phe Thr Ile Gln Asp Ser Ala Ser Tyr Asn Val Asn Ile Gln Ser Leu 
    210                 215                 220                 


Lys Phe Ala Gln Pro Leu Val Ser Ser Ser Gln Tyr Pro Ile Ala Asp 
225                 230                 235                 240 


Leu Thr Ser Ala Ile Asn Gly Thr Leu 
                245                 


<210>  5
<211>  747
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyt1Aa variant

<400>  5
atggaaaatt taaatcattg tccattagaa gatataaagg taaatccatg gaaaacccct       60

caatcaacag caagggttat tacattacgt gttgaggatc caaatgaaat caataatctt      120

ctttctatta acgaaattga taatccgaat tatatattgc aagcaattat gttagcaaat      180

tgttttcaaa atgcattagt tcccacttct acagattttg gtgatgccct acgctttagt      240

atgccaaaag gtttagaaat cgcaaacaca attacaccga tgggtgctgt agtgagttat      300

gttgatcaaa atgtaactca aacgaataac caagtaagtg ttatgattaa taaagtctta      360

gaagtgttaa aaactgtatt aggagttgca ttaagtggat ctgtaataga tcaattaact      420

gcagcagtta caaatacgtt tacaaattta aatactcaaa aaaatgaagc atggattttc      480

tggggcaagg aaactgctaa tcaaacaaat tacacataca atgtcctgtt tgcaatccaa      540

aatgcccaaa ctggtggcgt tatgtattgt gtaccagttg gttttgaaat taaagtatca      600

gcagtaaagg aacaagtttt atttttcaca attcaagatt ctgcgagcta caatgttaac      660

atccaatctt tgaaatttgc acaaccatta gttagctcaa gtcagtatcc aattgcagat      720

cttactagcg ctattaatgg aaccctc                                          747


<210>  6
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cyt1Aa variant

<400>  6

Met Glu Asn Leu Asn His Cys Pro Leu Glu Asp Ile Lys Val Asn Pro 
1               5                   10                  15      


Trp Lys Thr Pro Gln Ser Thr Ala Arg Val Ile Thr Leu Arg Val Glu 
            20                  25                  30          


Asp Pro Asn Glu Ile Asn Asn Leu Leu Ser Ile Asn Glu Ile Asp Asn 
        35                  40                  45              


Pro Asn Tyr Ile Leu Gln Ala Ile Met Leu Ala Asn Cys Phe Gln Asn 
    50                  55                  60                  


Ala Leu Val Pro Thr Ser Thr Asp Phe Gly Asp Ala Leu Arg Phe Ser 
65                  70                  75                  80  


Met Pro Lys Gly Leu Glu Ile Ala Asn Thr Ile Thr Pro Met Gly Ala 
                85                  90                  95      


Val Val Ser Tyr Val Asp Gln Asn Val Thr Gln Thr Asn Asn Gln Val 
            100                 105                 110         


Ser Val Met Ile Asn Lys Val Leu Glu Val Leu Lys Thr Val Leu Gly 
        115                 120                 125             


Val Ala Leu Ser Gly Ser Val Ile Asp Gln Leu Thr Ala Ala Val Thr 
    130                 135                 140                 


Asn Thr Phe Thr Asn Leu Asn Thr Gln Lys Asn Glu Ala Trp Ile Phe 
145                 150                 155                 160 


Trp Gly Lys Glu Thr Ala Asn Gln Thr Asn Tyr Thr Tyr Asn Val Leu 
                165                 170                 175     


Phe Ala Ile Gln Asn Ala Gln Thr Gly Gly Val Met Tyr Cys Val Pro 
            180                 185                 190         


Val Gly Phe Glu Ile Lys Val Ser Ala Val Lys Glu Gln Val Leu Phe 
        195                 200                 205             


Phe Thr Ile Gln Asp Ser Ala Ser Tyr Asn Val Asn Ile Gln Ser Leu 
    210                 215                 220                 


Lys Phe Ala Gln Pro Leu Val Ser Ser Ser Gln Tyr Pro Ile Ala Asp 
225                 230                 235                 240 


Leu Thr Ser Ala Ile Asn Gly Thr Leu 
                245                 


<210>  7
<211>  250
<212>  PRT
<213>  Bacillus thuringiensis

<400>  7

Met Glu Asn Pro Asn His Cys Pro Leu Glu Asp Ile Gln Val Asn Pro 
1               5                   10                  15      


Trp Lys Thr Pro Gln Ser Lys Ala Arg Val Ile Thr Leu Arg Ile Asp 
            20                  25                  30          


Asp Pro Asn Glu Ile Asn Asn Leu Leu Ser Ile Asn Glu Ile Glu Asn 
        35                  40                  45              


Thr Asn Tyr Leu Leu Gln Ala Ile Met Leu Ala Asn Ala Phe Gln Lys 
    50                  55                  60                  


Ala Leu Val Pro Thr Ser Thr Glu Phe Ala Glu Asp Ala Leu Gln Phe 
65                  70                  75                  80  


Ser Met Thr Lys Gly Leu Glu Val Ala Asn Thr Ile Ser Pro Pro Gly 
                85                  90                  95      


Ala Val Val Gln Tyr Val Asp Gln Asn Val Ser Gln Thr Asn Asn Gln 
            100                 105                 110         


Val Ser Ala Met Ile Asn Lys Val Leu Asp Val Leu Lys Ser Ile Leu 
        115                 120                 125             


Gly Val Ala Leu Gly Gln Ser Val Ile Glu Gln Leu Thr Ser Ala Val 
    130                 135                 140                 


Thr Asn Thr Phe Thr Asn Leu Asn Thr Gln Lys Asn Glu Ala Trp Ile 
145                 150                 155                 160 


Phe Trp Gly Arg Glu Thr Ser Thr Gln Thr Asn Tyr Thr Tyr Asn Val 
                165                 170                 175     


Leu Phe Ala Ile Gln Asn Gly Gln Thr Gly Gly Val Met Tyr Cys Val 
            180                 185                 190         


Pro Val Gly Phe Glu Ile Lys Val Ser Ala Val Lys Glu Arg Val Leu 
        195                 200                 205             


Phe Leu Thr Ile Gln Asp Ser Ala Ser Tyr Asn Val Asn Ile Gln Ser 
    210                 215                 220                 


Leu Lys Phe Ala Gln Pro Leu Val Ser Ala Ser Glu Tyr Pro Ile Ala 
225                 230                 235                 240 


Asp Leu Thr Ser Ala Ile Asn Gly Thr Leu 
                245                 250 


<210>  8
<211>  265
<212>  PRT
<213>  Bacillus thuringiensis

<400>  8

Met Lys Glu Ser Ile Tyr Tyr Asn Glu Glu Asn Glu Ile Gln Ile Ser 
1               5                   10                  15      


Gln Gly Asn Cys Phe Pro Glu Glu Leu Gly His Asn Pro Trp Arg Gln 
            20                  25                  30          


Pro Gln Ser Thr Ala Arg Val Ile Tyr Leu Lys Val Lys Asp Pro Ile 
        35                  40                  45              


Asp Thr Thr Gln Leu Leu Glu Ile Thr Glu Ile Glu Asn Pro Asn Tyr 
    50                  55                  60                  


Val Leu Gln Ala Ile Gln Leu Ala Ala Ala Phe Gln Asp Ala Leu Val 
65                  70                  75                  80  


Pro Thr Glu Thr Glu Phe Gly Glu Ala Ile Arg Phe Ser Met Pro Lys 
                85                  90                  95      


Gly Leu Glu Val Ala Lys Thr Ile Gln Pro Lys Gly Ala Val Val Ala 
            100                 105                 110         


Tyr Thr Asp Gln Thr Leu Ser Gln Ser Asn Asn Gln Val Ser Val Met 
        115                 120                 125             


Ile Asp Arg Val Ile Ser Val Leu Lys Thr Val Met Gly Val Ala Leu 
    130                 135                 140                 


Ser Gly Ser Ile Ile Thr Gln Leu Thr Ala Ala Ile Thr Asp Thr Phe 
145                 150                 155                 160 


Thr Asn Leu Asn Thr Gln Lys Asp Ser Ala Trp Val Phe Trp Gly Lys 
                165                 170                 175     


Glu Thr Ser His Gln Thr Asn Tyr Thr Tyr Asn Val Met Phe Ala Ile 
            180                 185                 190         


Gln Asn Glu Thr Thr Gly Arg Val Met Met Cys Val Pro Ile Gly Phe 
        195                 200                 205             


Glu Ile Arg Val Phe Thr Asp Lys Arg Thr Val Leu Phe Leu Thr Thr 
    210                 215                 220                 


Lys Asp Tyr Ala Asn Tyr Ser Val Asn Ile Gln Thr Leu Arg Phe Ala 
225                 230                 235                 240 


Gln Pro Leu Ile Asp Ser Arg Ala Leu Ser Ile Asn Asp Leu Ser Glu 
                245                 250                 255     


Ala Leu Arg Ser Ser Lys Tyr Leu Tyr 
            260                 265 


<210>  9
<211>  259
<212>  PRT
<213>  Bacillus thuringiensis

<400>  9

Met Tyr Thr Lys Asn Phe Ser Asn Ser Arg Met Glu Val Lys Gly Asn 
1               5                   10                  15      


Asn Gly Cys Ser Ala Pro Ile Ile Arg Lys Pro Phe Lys His Ile Val 
            20                  25                  30          


Leu Thr Val Pro Ser Ser Asp Leu Asp Asn Phe Asn Thr Val Phe Tyr 
        35                  40                  45              


Val Gln Pro Gln Tyr Ile Asn Gln Ala Leu His Leu Ala Asn Ala Phe 
    50                  55                  60                  


Gln Gly Ala Ile Asp Pro Leu Asn Leu Asn Phe Asn Phe Glu Lys Ala 
65                  70                  75                  80  


Leu Gln Ile Ala Asn Gly Ile Pro Asn Ser Ala Ile Val Lys Thr Leu 
                85                  90                  95      


Asn Gln Ser Val Ile Gln Gln Thr Val Glu Ile Ser Val Met Val Glu 
            100                 105                 110         


Gln Leu Lys Lys Ile Ile Gln Glu Val Leu Gly Leu Val Ile Asn Ser 
        115                 120                 125             


Thr Ser Phe Trp Asn Ser Val Glu Ala Thr Ile Lys Gly Thr Phe Thr 
    130                 135                 140                 


Asn Leu Asp Thr Gln Ile Asp Glu Ala Trp Ile Phe Trp His Ser Leu 
145                 150                 155                 160 


Ser Ala His Asn Thr Ser Tyr Tyr Tyr Asn Ile Leu Phe Ser Ile Gln 
                165                 170                 175     


Asn Glu Asp Thr Gly Ala Val Met Ala Val Leu Pro Leu Ala Phe Glu 
            180                 185                 190         


Val Ser Val Asp Val Glu Lys Gln Lys Val Leu Phe Phe Thr Ile Lys 
        195                 200                 205             


Asp Ser Ala Arg Tyr Glu Val Lys Met Lys Ala Leu Thr Leu Val Gln 
    210                 215                 220                 


Ala Leu His Ser Ser Asn Ala Pro Ile Val Asp Ile Phe Asn Val Asn 
225                 230                 235                 240 


Asn Tyr Asn Leu Tyr His Ser Asn His Lys Ile Ile Gln Asn Leu Asn 
                245                 250                 255     


Leu Ser Asn 
            


<210>  10
<211>  263
<212>  PRT
<213>  Bacillus thuringiensis

<400>  10

Met His Leu Asn Asn Leu Asn Asn Phe Asn Asn Leu Glu Asn Asn Gly 
1               5                   10                  15      


Glu Tyr His Cys Ser Gly Pro Ile Ile Lys Lys Pro Phe Arg His Ile 
            20                  25                  30          


Ala Leu Thr Val Pro Ser Ser Asp Ile Thr Asn Phe Asn Glu Ile Phe 
        35                  40                  45              


Tyr Val Glu Pro Gln Tyr Ile Ala Gln Ala Ile Arg Leu Thr Asn Thr 
    50                  55                  60                  


Phe Gln Gly Ala Ile Asp Pro Leu Thr Leu Asn Phe Asn Phe Glu Lys 
65                  70                  75                  80  


Ala Leu Gln Ile Ala Asn Gly Leu Pro Asn Ala Gly Val Thr Gly Thr 
                85                  90                  95      


Ile Asn Gln Ser Val Ile His Gln Thr Ile Glu Val Ser Val Met Ile 
            100                 105                 110         


Ser Gln Ile Lys Glu Ile Ile Arg Ser Val Leu Gly Leu Val Ile Asn 
        115                 120                 125             


Ser Ala Asn Phe Trp Asn Ser Val Val Ser Ala Ile Thr Asn Thr Phe 
    130                 135                 140                 


Thr Asn Leu Glu Pro Gln Val Asp Glu Asn Trp Ile Val Trp Arg Asn 
145                 150                 155                 160 


Leu Ser Ala Thr Gln Thr Ser Tyr Phe Tyr Lys Ile Leu Phe Ser Ile 
                165                 170                 175     


Gln Asn Glu Asp Thr Gly Arg Phe Met Ala Ile Leu Pro Ile Ala Phe 
            180                 185                 190         


Glu Ile Thr Val Asp Val Gln Lys Gln Gln Leu Leu Phe Ile Thr Ile 
        195                 200                 205             


Lys Asp Ser Ala Arg Tyr Glu Val Lys Met Lys Ala Leu Thr Val Val 
    210                 215                 220                 


Gln Ala Leu Asp Ser Tyr Asn Ala Pro Ile Ile Asp Val Phe Asn Val 
225                 230                 235                 240 


Arg Asn Tyr Ser Leu His Arg Pro Asn His Asn Ile Leu Gln Asn Leu 
                245                 250                 255     


Asn Val Asn Pro Ile Lys Ser 
            260             


<210>  11
<211>  263
<212>  PRT
<213>  Bacillus thuringiensis

<400>  11

Met Tyr Thr Lys Asn Leu Asn Ser Leu Glu Ile Asn Glu Asp Tyr Gln 
1               5                   10                  15      


Tyr Ser Arg Pro Ile Ile Lys Lys Pro Phe Arg His Ile Thr Leu Thr 
            20                  25                  30          


Val Pro Ser Ser Asp Ile Ala Ser Phe Asn Glu Ile Phe Tyr Leu Glu 
        35                  40                  45              


Pro Gln Tyr Val Ala Gln Ala Leu Arg Leu Thr Asn Thr Phe Gln Ala 
    50                  55                  60                  


Ala Ile Asp Pro Leu Thr Leu Asn Phe Asp Phe Glu Lys Ala Leu Gln 
65                  70                  75                  80  


Ile Ala Asn Gly Leu Pro Asn Ala Gly Ile Thr Gly Thr Leu Asn Gln 
                85                  90                  95      


Ser Val Ile Gln Gln Thr Ile Glu Ile Ser Val Met Ile Ser Gln Ile 
            100                 105                 110         


Lys Glu Ile Ile Arg Asn Val Leu Gly Leu Val Ile Asn Ser Thr Asn 
        115                 120                 125             


Phe Trp Asn Ser Val Leu Ala Ala Ile Thr Asn Thr Phe Thr Asn Leu 
    130                 135                 140                 


Glu Pro Gln Val Asp Glu Asn Trp Ile Val Trp Arg Asn Leu Ser Ala 
145                 150                 155                 160 


Thr His Thr Ser Tyr Tyr Tyr Lys Ile Leu Phe Ser Ile Gln Asn Glu 
                165                 170                 175     


Asp Thr Gly Ala Phe Met Ala Val Leu Pro Ile Ala Phe Glu Ile Thr 
            180                 185                 190         


Val Asp Val Gln Lys Gln Gln Leu Leu Phe Ile Thr Ile Arg Asp Ser 
        195                 200                 205             


Ala Arg Tyr Glu Val Lys Met Lys Ala Leu Thr Val Val Gln Leu Leu 
    210                 215                 220                 


Asp Ser Tyr Asn Ala Pro Ile Ile Asp Val Phe Asn Val His Asn Tyr 
225                 230                 235                 240 


Gly Leu Tyr Gln Ser Asn His Pro Asn His His Ile Leu Gln Asn Leu 
                245                 250                 255     


Asn Leu Asn Lys Ile Lys Gly 
            260             


<210>  12
<211>  260
<212>  PRT
<213>  Bacillus thuringiensis

<400>  12

Met Tyr Ile Asn Asn Phe Asp Phe Pro Glu Lys Asn Asn Asp Tyr Gln 
1               5                   10                  15      


Cys Ser Gly Pro Ile Ile Lys Lys Pro Phe Arg His Ile Ala Leu Thr 
            20                  25                  30          


Val Pro Ser Ser Asp Ile Thr Asn Phe Asn Glu Ile Phe Tyr Val Glu 
        35                  40                  45              


Pro Gln Tyr Ile Ala Gln Ala Leu Arg Leu Thr Asn Thr Phe Gln Gly 
    50                  55                  60                  


Ala Ile Asp Pro Leu Thr Leu Asn Phe Asn Phe Glu Lys Ala Leu Gln 
65                  70                  75                  80  


Ile Ala Asn Gly Leu Pro Asn Ala Gly Val Thr Gly Thr Leu Asn Gln 
                85                  90                  95      


Ser Val Ile His Gln Thr Ile Glu Ile Ser Val Met Ile Ser Gln Ile 
            100                 105                 110         


Lys Glu Ile Ile Arg Ser Val Leu Gly Leu Val Ile Asn Ser Ala Asn 
        115                 120                 125             


Phe Trp Asn Asn Val Val Ser Ala Ile Thr Asn Thr Phe Thr Asn Leu 
    130                 135                 140                 


Glu Pro Gln Val Asp Glu Asn Trp Ile Val Trp Arg Asn Leu Ser Ala 
145                 150                 155                 160 


Asn Gln Thr Ser Tyr Tyr Tyr Lys Ile Leu Phe Ser Ile Gln Asn Glu 
                165                 170                 175     


Asp Thr Gly Arg Phe Met Ala Val Leu Pro Ile Ala Phe Glu Ile Asn 
            180                 185                 190         


Val Asp Val His Lys Gln Gln Leu Leu Phe Ile Thr Ile Lys Asp Ser 
        195                 200                 205             


Ala Arg Tyr Glu Val Lys Met Lys Ala Leu Thr Val Val Gln Ala Leu 
    210                 215                 220                 


Asp Ser Tyr Asn Ala Pro Ile Ile Asp Val Phe Asn Ile His Asn Tyr 
225                 230                 235                 240 


Ser Leu His Arg Pro Asn Tyr His Ile Leu Gln Asn Leu Asn Val Asn 
                245                 250                 255     


Pro Ile Lys Ser 
            260 


<210>  13
<211>  643
<212>  PRT
<213>  Bacillus thuringiensis

<400>  13

Met Glu Asp Ser Ser Leu Asp Thr Leu Ser Ile Val Asn Glu Thr Asp 
1               5                   10                  15      


Phe Pro Leu Tyr Asn Asn Tyr Thr Glu Pro Thr Ile Ala Pro Ala Leu 
            20                  25                  30          


Ile Ala Val Ala Pro Ile Ala Gln Tyr Leu Ala Thr Ala Ile Gly Lys 
        35                  40                  45              


Trp Ala Ala Lys Ala Ala Phe Ser Lys Val Leu Ser Leu Ile Phe Pro 
    50                  55                  60                  


Gly Ser Gln Pro Ala Thr Met Glu Lys Val Arg Thr Glu Val Glu Thr 
65                  70                  75                  80  


Leu Ile Asn Gln Lys Leu Ser Gln Asp Arg Val Asn Ile Leu Asn Ala 
                85                  90                  95      


Glu Tyr Arg Gly Ile Ile Glu Val Ser Asp Val Phe Asp Ala Tyr Ile 
            100                 105                 110         


Lys Gln Pro Gly Phe Thr Pro Ala Thr Ala Lys Gly Tyr Phe Leu Asn 
        115                 120                 125             


Leu Ser Gly Ala Ile Ile Gln Arg Leu Pro Gln Phe Glu Val Gln Thr 
    130                 135                 140                 


Tyr Glu Gly Val Ser Ile Ala Leu Phe Thr Gln Met Cys Thr Leu His 
145                 150                 155                 160 


Leu Thr Leu Leu Lys Asp Gly Ile Leu Ala Gly Ser Ala Trp Gly Phe 
                165                 170                 175     


Thr Gln Ala Asp Val Asp Ser Phe Ile Lys Leu Phe Asn Gln Lys Val 
            180                 185                 190         


Leu Asp Tyr Arg Thr Arg Leu Met Arg Met Tyr Thr Glu Glu Phe Gly 
        195                 200                 205             


Arg Leu Cys Lys Val Ser Leu Lys Asp Gly Leu Thr Phe Arg Asn Met 
    210                 215                 220                 


Cys Asn Leu Tyr Val Phe Pro Phe Ala Glu Ala Trp Ser Leu Met Arg 
225                 230                 235                 240 


Tyr Glu Gly Leu Lys Leu Gln Ser Ser Leu Ser Leu Trp Asp Tyr Val 
                245                 250                 255     


Gly Val Ser Ile Pro Val Asn Tyr Asn Glu Trp Gly Gly Leu Val Tyr 
            260                 265                 270         


Lys Leu Leu Met Gly Glu Val Asn Gln Arg Leu Thr Thr Val Lys Phe 
        275                 280                 285             


Asn Tyr Ser Phe Thr Asn Glu Pro Ala Asp Ile Pro Ala Arg Glu Asn 
    290                 295                 300                 


Ile Arg Gly Val His Pro Ile Tyr Asp Pro Ser Ser Gly Leu Thr Gly 
305                 310                 315                 320 


Trp Ile Gly Asn Gly Arg Thr Asn Asn Phe Asn Phe Ala Asp Asn Asn 
                325                 330                 335     


Gly Asn Glu Ile Met Glu Val Arg Thr Gln Thr Phe Tyr Gln Asn Pro 
            340                 345                 350         


Asn Asn Glu Pro Ile Ala Pro Arg Asp Ile Ile Asn Gln Ile Leu Thr 
        355                 360                 365             


Ala Pro Ala Pro Ala Asp Leu Phe Phe Lys Asn Ala Asp Ile Asn Val 
    370                 375                 380                 


Lys Phe Thr Gln Trp Phe Gln Ser Thr Leu Tyr Gly Trp Asn Ile Lys 
385                 390                 395                 400 


Leu Gly Thr Gln Thr Val Leu Ser Ser Arg Thr Gly Thr Ile Pro Pro 
                405                 410                 415     


Asn Tyr Leu Ala Tyr Asp Gly Tyr Tyr Ile Arg Ala Ile Ser Ala Cys 
            420                 425                 430         


Pro Arg Gly Val Ser Leu Ala Tyr Asn His Asp Leu Thr Thr Leu Thr 
        435                 440                 445             


Tyr Asn Arg Ile Glu Tyr Asp Ser Pro Thr Thr Glu Asn Ile Ile Val 
    450                 455                 460                 


Gly Phe Ala Pro Asp Asn Thr Lys Asp Phe Tyr Ser Lys Lys Ser His 
465                 470                 475                 480 


Tyr Leu Ser Glu Thr Asn Asp Ser Tyr Val Ile Pro Ala Leu Gln Phe 
                485                 490                 495     


Ala Glu Val Ser Asp Arg Ser Phe Leu Glu Asp Thr Pro Asp Gln Ala 
            500                 505                 510         


Thr Asp Gly Ser Ile Lys Phe Ala Arg Thr Phe Ile Ser Asn Glu Ala 
        515                 520                 525             


Lys Tyr Ser Ile Arg Leu Asn Thr Gly Phe Asn Thr Ala Thr Arg Tyr 
    530                 535                 540                 


Lys Leu Ile Ile Arg Val Arg Val Pro Tyr Arg Leu Pro Ala Gly Ile 
545                 550                 555                 560 


Arg Val Gln Ser Gln Asn Ser Gly Asn Asn Arg Met Leu Gly Ser Phe 
                565                 570                 575     


Thr Ala Asn Ala Asn Pro Glu Trp Val Asp Phe Val Thr Asp Ala Phe 
            580                 585                 590         


Thr Phe Asn Asp Leu Gly Ile Thr Thr Ser Ser Thr Asn Ala Leu Phe 
        595                 600                 605             


Ser Ile Ser Ser Asp Ser Leu Asn Ser Gly Glu Glu Trp Tyr Leu Ser 
    610                 615                 620                 


Gln Leu Phe Leu Val Lys Glu Ser Ala Phe Thr Thr Gln Ile Asn Pro 
625                 630                 635                 640 


Leu Leu Lys 
            


<210>  14
<211>  747
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyt1Aa variant

<400>  14
atggaaaatt taaatcattg tccattagaa gatataaagg taaatccatg gaaaacccct       60

caatcaacag caagggttat tacattacgt gttgaggatc caaatgaaat caataatctt      120

ctttctatta acgaaattga taatccgaat tatatattgc aagcaattat ggaagcaaat      180

gcatttcaaa atgcattagt tcccacttct acagattttg gtgatgccct acgctttagt      240

atgccaaaag gtttagaaat cgcaaacaca attacaccga tgggtgctgt agtgagttat      300

gttgatcaaa atgtaactca aacgaataac caagtaagtg ttatgattaa taaagtctta      360

gaagtgttaa aaactgtatt aggagttgca ttaagtggat ctgtaataga tcaattaact      420

gcagcagtta caaatacgtt tacaaattta aatactcaaa aaaatgaagc atggattttc      480

tggggcaagg aaactgctaa tcaaacaaat tacacataca atgtcctgtt tgcaatccaa      540

aatgcccaaa ctggtggcgt tatgtattgt gtaccagttg gttttgaaat taaagtatca      600

gcagtaaagg aacaagtttt atttttcaca attcaagatt ctgcgagcta caatgttaac      660

atccaatctt tgaaatttgc acaaccatta gttagctcaa gtcagtatcc aattgcagat      720

cttactagcg ctattaatgg aaccctc                                          747


<210>  15
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cyt1Aa variant

<400>  15

Met Glu Asn Leu Asn His Cys Pro Leu Glu Asp Ile Lys Val Asn Pro 
1               5                   10                  15      


Trp Lys Thr Pro Gln Ser Thr Ala Arg Val Ile Thr Leu Arg Val Glu 
            20                  25                  30          


Asp Pro Asn Glu Ile Asn Asn Leu Leu Ser Ile Asn Glu Ile Asp Asn 
        35                  40                  45              


Pro Asn Tyr Ile Leu Gln Ala Ile Met Glu Ala Asn Ala Phe Gln Asn 
    50                  55                  60                  


Ala Leu Val Pro Thr Ser Thr Asp Phe Gly Asp Ala Leu Arg Phe Ser 
65                  70                  75                  80  


Met Pro Lys Gly Leu Glu Ile Ala Asn Thr Ile Thr Pro Met Gly Ala 
                85                  90                  95      


Val Val Ser Tyr Val Asp Gln Asn Val Thr Gln Thr Asn Asn Gln Val 
            100                 105                 110         


Ser Val Met Ile Asn Lys Val Leu Glu Val Leu Lys Thr Val Leu Gly 
        115                 120                 125             


Val Ala Leu Ser Gly Ser Val Ile Asp Gln Leu Thr Ala Ala Val Thr 
    130                 135                 140                 


Asn Thr Phe Thr Asn Leu Asn Thr Gln Lys Asn Glu Ala Trp Ile Phe 
145                 150                 155                 160 


Trp Gly Lys Glu Thr Ala Asn Gln Thr Asn Tyr Thr Tyr Asn Val Leu 
                165                 170                 175     


Phe Ala Ile Gln Asn Ala Gln Thr Gly Gly Val Met Tyr Cys Val Pro 
            180                 185                 190         


Val Gly Phe Glu Ile Lys Val Ser Ala Val Lys Glu Gln Val Leu Phe 
        195                 200                 205             


Phe Thr Ile Gln Asp Ser Ala Ser Tyr Asn Val Asn Ile Gln Ser Leu 
    210                 215                 220                 


Lys Phe Ala Gln Pro Leu Val Ser Ser Ser Gln Tyr Pro Ile Ala Asp 
225                 230                 235                 240 


Leu Thr Ser Ala Ile Asn Gly Thr Leu 
                245                 


<210>  16
<211>  747
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyt1Aa variant

<400>  16
atggaaaatt taaatcattg tccattagaa gatataaagg taaatccatg gaaaacccct       60

caatcaacag caagggttat tacattacgt gttgaggatc caaatgaaat caataatctt      120

ctttctatta acgaaattga taatccgaat tatatattgc aagcaattat gttagaaaat      180

gcatttcaaa atgcattagt tcccacttct acagattttg gtgatgccct acgctttagt      240

atgccaaaag gtttagaaat cgcaaacaca attacaccga tgggtgctgt agtgagttat      300

gttgatcaaa atgtaactca aacgaataac caagtaagtg ttatgattaa taaagtctta      360

gaagtgttaa aaactgtatt aggagttgca ttaagtggat ctgtaataga tcaattaact      420

gcagcagtta caaatacgtt tacaaattta aatactcaaa aaaatgaagc atggattttc      480

tggggcaagg aaactgctaa tcaaacaaat tacacataca atgtcctgtt tgcaatccaa      540

aatgcccaaa ctggtggcgt tatgtattgt gtaccagttg gttttgaaat taaagtatca      600

gcagtaaagg aacaagtttt atttttcaca attcaagatt ctgcgagcta caatgttaac      660

atccaatctt tgaaatttgc acaaccatta gttagctcaa gtcagtatcc aattgcagat      720

cttactagcg ctattaatgg aaccctc                                          747


<210>  17
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cyt1Aa variant

<400>  17

Met Glu Asn Leu Asn His Cys Pro Leu Glu Asp Ile Lys Val Asn Pro 
1               5                   10                  15      


Trp Lys Thr Pro Gln Ser Thr Ala Arg Val Ile Thr Leu Arg Val Glu 
            20                  25                  30          


Asp Pro Asn Glu Ile Asn Asn Leu Leu Ser Ile Asn Glu Ile Asp Asn 
        35                  40                  45              


Pro Asn Tyr Ile Leu Gln Ala Ile Met Leu Glu Asn Ala Phe Gln Asn 
    50                  55                  60                  


Ala Leu Val Pro Thr Ser Thr Asp Phe Gly Asp Ala Leu Arg Phe Ser 
65                  70                  75                  80  


Met Pro Lys Gly Leu Glu Ile Ala Asn Thr Ile Thr Pro Met Gly Ala 
                85                  90                  95      


Val Val Ser Tyr Val Asp Gln Asn Val Thr Gln Thr Asn Asn Gln Val 
            100                 105                 110         


Ser Val Met Ile Asn Lys Val Leu Glu Val Leu Lys Thr Val Leu Gly 
        115                 120                 125             


Val Ala Leu Ser Gly Ser Val Ile Asp Gln Leu Thr Ala Ala Val Thr 
    130                 135                 140                 


Asn Thr Phe Thr Asn Leu Asn Thr Gln Lys Asn Glu Ala Trp Ile Phe 
145                 150                 155                 160 


Trp Gly Lys Glu Thr Ala Asn Gln Thr Asn Tyr Thr Tyr Asn Val Leu 
                165                 170                 175     


Phe Ala Ile Gln Asn Ala Gln Thr Gly Gly Val Met Tyr Cys Val Pro 
            180                 185                 190         


Val Gly Phe Glu Ile Lys Val Ser Ala Val Lys Glu Gln Val Leu Phe 
        195                 200                 205             


Phe Thr Ile Gln Asp Ser Ala Ser Tyr Asn Val Asn Ile Gln Ser Leu 
    210                 215                 220                 


Lys Phe Ala Gln Pro Leu Val Ser Ser Ser Gln Tyr Pro Ile Ala Asp 
225                 230                 235                 240 


Leu Thr Ser Ala Ile Asn Gly Thr Leu 
                245                 


<210>  18
<211>  747
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyt1Aa variant

<400>  18
atggaaaatt taaatcattg tccattagaa gatataaagg taaatccatg gaaaacccct       60

caatcaacag caagggttat tacattacgt gttgaggatc caaatgaaat caataatctt      120

ctttctatta acgaaattga taatccgaat tatatattgc aagcaattat gttagcaaat      180

gcacggcaaa atgcattagt tcccacttct acagattttg gtgatgccct acgctttagt      240

atgccaaaag gtttagaaat cgcaaacaca attacaccga tgggtgctgt agtgagttat      300

gttgatcaaa atgtaactca aacgaataac caagtaagtg ttatgattaa taaagtctta      360

gaagtgttaa aaactgtatt aggagttgca ttaagtggat ctgtaataga tcaattaact      420

gcagcagtta caaatacgtt tacaaattta aatactcaaa aaaatgaagc atggattttc      480

tggggcaagg aaactgctaa tcaaacaaat tacacataca atgtcctgtt tgcaatccaa      540

aatgcccaaa ctggtggcgt tatgtattgt gtaccagttg gttttgaaat taaagtatca      600

gcagtaaagg aacaagtttt atttttcaca attcaagatt ctgcgagcta caatgttaac      660

atccaatctt tgaaatttgc acaaccatta gttagctcaa gtcagtatcc aattgcagat      720

cttactagcg ctattaatgg aaccctc                                          747


<210>  19
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cyt1Aa variant

<400>  19

Met Glu Asn Leu Asn His Cys Pro Leu Glu Asp Ile Lys Val Asn Pro 
1               5                   10                  15      


Trp Lys Thr Pro Gln Ser Thr Ala Arg Val Ile Thr Leu Arg Val Glu 
            20                  25                  30          


Asp Pro Asn Glu Ile Asn Asn Leu Leu Ser Ile Asn Glu Ile Asp Asn 
        35                  40                  45              


Pro Asn Tyr Ile Leu Gln Ala Ile Met Leu Ala Asn Ala Arg Gln Asn 
    50                  55                  60                  


Ala Leu Val Pro Thr Ser Thr Asp Phe Gly Asp Ala Leu Arg Phe Ser 
65                  70                  75                  80  


Met Pro Lys Gly Leu Glu Ile Ala Asn Thr Ile Thr Pro Met Gly Ala 
                85                  90                  95      


Val Val Ser Tyr Val Asp Gln Asn Val Thr Gln Thr Asn Asn Gln Val 
            100                 105                 110         


Ser Val Met Ile Asn Lys Val Leu Glu Val Leu Lys Thr Val Leu Gly 
        115                 120                 125             


Val Ala Leu Ser Gly Ser Val Ile Asp Gln Leu Thr Ala Ala Val Thr 
    130                 135                 140                 


Asn Thr Phe Thr Asn Leu Asn Thr Gln Lys Asn Glu Ala Trp Ile Phe 
145                 150                 155                 160 


Trp Gly Lys Glu Thr Ala Asn Gln Thr Asn Tyr Thr Tyr Asn Val Leu 
                165                 170                 175     


Phe Ala Ile Gln Asn Ala Gln Thr Gly Gly Val Met Tyr Cys Val Pro 
            180                 185                 190         


Val Gly Phe Glu Ile Lys Val Ser Ala Val Lys Glu Gln Val Leu Phe 
        195                 200                 205             


Phe Thr Ile Gln Asp Ser Ala Ser Tyr Asn Val Asn Ile Gln Ser Leu 
    210                 215                 220                 


Lys Phe Ala Gln Pro Leu Val Ser Ser Ser Gln Tyr Pro Ile Ala Asp 
225                 230                 235                 240 


Leu Thr Ser Ala Ile Asn Gly Thr Leu 
                245                 


<210>  20
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutagenesis primer

<400>  20
ttgcaagcaa ttatgttatg taatgccttt caaaatgc                               38


<210>  21
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutagenesis primer

<400>  21
gcaagcaatt atgttagcaa actgttttca aaatgcatta gttccc                      46


<210>  22
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutagenesis primer

<400>  22
tatatattgc aagcaattat ggaagcaaat gcgtttcaaa atgc                        44


<210>  23
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutagenesis primer

<400>  23
tatattgcaa gcaattatgt tagaaaatgc gtttcaaaat gc                          42


<210>  24
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutagenesis primer

<400>  24
agcaattatg ttagcaaatg cacggcaaaa tgcgttagtt cc                          42


<210>  25
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutagenesis primer

<400>  25
tgtgaattca tggaaaattt aaatcattg                                         29


<210>  26
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutagenesis primer

<400>  26
ctactcgagg agggttccat taatagc                                           27


