                         SEQUENCE LISTING

<110>  BioMadison, Inc.
 
<120>  IMPROVED ASSAY WITH SYNAPTOBREVIN BASED MOIETY

<130>  102320.0024PCT

<150>  US 62/404513
<151>  2016-10-05

<150>  US 62/336964
<151>  2016-05-16

<160>  23    

<170>  PatentIn version 3.5

<210>  1
<211>  1077
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMD0032 YFPVAMP2, plasmid encoding for YFPVAMP2 construct

<400>  1
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagt      720

ggaggcatgt cggctaccgc tgccaccgtc ccgcctgccg ccccggccgg cgagggtggc      780

ccccctgcac ctcctccaaa tcttaccagt aacaggagac tgcagcagac ccaggcccag      840

gtggatgagg tggtggacat catgagggtg aatgtggaca aggtcctgga gcgrgaccag      900

aagctatcgg aactggatga tcgcgcagat gccctccagg caggggcctc ccagtttgaa      960

acaagtgcag ccaagctcaa gcgcaaatac tggtggaaaa acctcaagat gatgatcatc     1020

ttgggagtga tttgcgccat catcctcatc atcatcatcg tttacttcag cacttaa        1077


<210>  2
<211>  1077
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMD0034 ECFPVAMP2, plasmid encoding for ECFPVAMP2 construct

<400>  2
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac      480

ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagt      720

ggaggcatgt cggctaccgc tgccaccgtc ccgcctgccg ccccggccgg cgagggtggc      780

ccccctgcac ctcctccaaa tcttaccagt aacaggagac tgcagcagac ccaggcccag      840

gtggatgagg tggtggacat catgagggtg aatgtggaca aggtcctgga gcgrgaccag      900

aagctatcgg aactggatga tcgcgcagat gccctccagg caggggcctc ccagtttgaa      960

acaagtgcag ccaagctcaa gcgcaaatac tggtggaaaa acctcaagat gatgatcatc     1020

ttgggagtga tttgcgccat catcctcatc atcatcatcg tttacttcag cacttaa        1077


<210>  3
<211>  1076
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMD0034 YFPVAMP2, plasmid encoding for YFPVAMP2 construct

<400>  3
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagt      720

ggaggcatgt cggctaccgc tgccaccgtc ccgcctgccg ccccggccgg cgagggtggc      780

cccctgcacc tcctccaaat cttaccagta acaggagact gcagcagacc caggcccagg      840

tggatgaggt ggtggacatc atgagggtga atgtggacaa ggtcctggag cgrgaccaga      900

agctatcgga actggatgat cgcgcagatg ccctccaggc aggggcctcc cagtttgaaa      960

caagtgcagc caagctcaag cgcaaatact ggtggaaaaa cctcaagatg atgatcatct     1020

tgggagtgat ttgcgccatc atcctcatca tcatcatcgt ttacttcagc acttaa         1076


<210>  4
<211>  1077
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMD0071 ECFPVAMP2, plasmid encoding for ECFPVAMP2 construct

<400>  4
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac      480

ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagt      720

ggaggcatgt cggctaccgc tgccaccgtc ccgcctgccg ccccggccgg cgagggtggc      780

ccccctgcac ctcctccaaa tcttaccagt aacaggagac tgcagcagac ccaggcccag      840

gtggatgagg tggtggacat catgagggtg aatgtggaca aggtcctgga gcgrgaccag      900

aagctatcgg aactggatga tcgcgcagat gccctccagg caggggcctc cgtgtttgaa      960

acaagtgcag ccaagctcaa gcgcaaatac tggtggaaaa acctcaagat gatgatcatc     1020

ttgggagtga tttgcgccat catcctcatc atcatcatcg tttacttcag cacttaa        1077


<210>  5
<211>  1077
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMD0071 YFPVAMP2, plasmid encoding for YFPVAMP2 construct

<400>  5
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagt      720

ggaggcatgt cggctaccgc tgccaccgtc ccgcctgccg ccccggccgg cgagggtggc      780

ccccctgcac ctcctccaaa tcttaccagt aacaggagac tgcagcagac ccaggcccag      840

gtggatgagg tggtggacat catgagggtg aatgtggaca aggtcctgga gcgrgaccag      900

aagctatcgg aactggatga tcgcgcagat gccctccagg caggggcctc ccagtttgaa      960

acaagtgcag ccaagctcaa gcgcaaatac tggtggaaaa acctcaagat gatgatcatc     1020

ttgggagtga tttgcgccat catcctcatc atcatcatcg tttacttcag cacttaa        1077


<210>  6
<211>  1077
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMD0185 ECFPVAMP2, plasmid encoding for ECFPVAMP2 construct

<400>  6
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac      480

ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagt      720

ggaggcatgt cggctaccgc tgccaccgtc ccgcctgccg ccccggccgg cgagggtggc      780

ccccctgcac ctcctccaaa tcttaccagt aacaggagac tgcagcagac ccaggcccag      840

gtggatgagg tggtggacat catgagggtg aatgtggaca aggtcctgga gcgrgaccag      900

aagctatcgg aactgaataa tcgcgcaaat gccctccagg caggggcctc cgtgtttgaa      960

acaagtgcag ccaagctcaa gcgcaaatac tggtggaaaa acctcaagat gatgatcatc     1020

ttgggagtga tttgcgccat catcctcatc atcatcatcg tttacttcag cacttaa        1077


<210>  7
<211>  843
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMD0185 YFPVAMP2, plasmid encoding for YFPVAMP2 construct

<400>  7
atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc       60

atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac      120

accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg      180

gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag      240

aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag      300

ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac      360

aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac      420

atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac      480

aagagtggag gcatgtcggc taccgctgcc accgtcccgc ctgccgcccc ggccggcgag      540

ggtggccccc ctgcacctcc tccaaatctt accagtaaca ggagactgca gcagacccag      600

gcccaggtgg atgaggtggt ggacatcatg agggtgaatg tggacaaggt cctggagcgr      660

gaccagaagc tatcggaact ggatgatcgc gcagatgccc tccaggcagg ggcctcccag      720

tttgaaacaa gtgcagccaa gctcaagcgc aaatactggt ggaaaaacct caagatgatg      780

atcatcttgg gagtgatttg cgccatcatc ctcatcatca tcatcgttta cttcagcact      840

taa                                                                    843


<210>  8
<211>  1812
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMD0183 YFPYFPVAMP2, encoding a construct containing two YFP 
       sequences

<400>  8
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtct      720

ggaggcaagc ttgcaatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc      780

ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag      840

ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc      900

gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt cgcccgctac      960

cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag     1020

gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc     1080

gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc     1140

aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc     1200

gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc     1260

agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg     1320

ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag     1380

cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac     1440

gagctgtaca agagtggagg catgtcggct accgctgcca ccgtcccgcc tgccgccccg     1500

gccggcgagg gtggcccccc tgcacctcct ccaaatctta ccagtaacag gagactgcag     1560

cagacccagg cccaggtgga tgaggtggtg gacatcatga gggtgaatgt ggacaaggtc     1620

ctggagcgag accagaagct atcggaactg gatgatcgcg cagatgccct ccaggcaggg     1680

gcctcccagt ttgaaacaag tgcagccaag ctcaagcgca aatactggtg gaaaaacctc     1740

aagatgatga tcatcttggg agtgatttgc gccatcatcc tcatcatcat catcgtttac     1800

ttcagcactt aa                                                         1812


<210>  9
<211>  1116
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMD0191, plasmid encoding a YFP-bearing construct that includes a
       p53 degron sequence

<400>  9
atgggcaaag gttcctacgg ttccggtggc aagcttgcaa tggtgagcaa gggcgaggag       60

ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag      120

ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc      180

atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac      240

ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc      300

gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac      360

aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag      420

ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac      480

agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag      540

atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc      600

cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc      660

ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc      720

gccgggatca ctctcggcat ggacgagctg tacaagagtg gaggcatgtc ggctaccgct      780

gccaccgtcc cgcctgccgc cccggccggc gagggtggcc cccctgcacc tcctccaaat      840

cttaccagta acaggagact gcagcagacc caggcccagg tggatgaggt ggtggacatc      900

atgagggtga atgtggacaa ggtcctggag cgagaccaga agctatcgga actggatgat      960

cgcgcagatg ccctccaggc aggggcctcc cagtttgaaa caagtgcagc caagctcaag     1020

cgcaaatact ggtggaaaaa cctcaagatg atgatcatct tgggagtgat ttgcgccatc     1080

atcctcatca tcatcatcgt ttacttcagc acttaa                               1116


<210>  10
<211>  1013
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMD0192, plasmid encoding a YFP-bearing construct including a 
       SNAP-25 degron sequence

<400>  10
atgggccgtg caacaaagat gctgggaagt ggttcgaatt ctggtggttc taagcttgca       60

atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac      120

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      180

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      240

ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag      300

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      360

ttcaaggacg acacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca      420

tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc      480

actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc      540

tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc      600

tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagagtggag      660

gcatgtcggc taccgctgcc accgtcccgc ctgccgcccc ggccggcgag ggtggccccc      720

ctgcacctcc tccaaatctt accagtaaca ggagactgca gcagacccag gcccaggtgg      780

atgaggtggt ggacatcatg agggtgaatg tggacaaggt cctggagcgg gaccagaagt      840

tgtcggagct ggatgaccgt gcagatgccc tccaggcagg ggcctcccag tttgaaacaa      900

gtgcagccaa gctcaagcgc aaatactggt ggaaaaacct caagatgatg atcatcttgg      960

gagtgatctg cgccatcatc ctcatcatca tcatcgttta cttcagcact taa            1013


<210>  11
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide incorporating Bonger degron sequence

<400>  11

Lys Thr Arg Gly Val Glu Glu Val Ala Glu Gly Val Val Leu Leu Arg 
1               5                   10                  15      


Arg Arg Gly Asn Lys 
            20      


<210>  12
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide incorporating TAZ degron

<400>  12

Lys Pro Phe Leu Asn Gly Gly Pro Tyr His Ser Arg Glu Gln Ser Thr 
1               5                   10                  15      


Asp Ser Gly Leu Gly Leu Gly Ser Tyr Lys 
            20                  25      


<210>  13
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide incorporating HIF-alpha degron

<400>  13

Ala Ser Ala Asp Leu Asp Leu Glu Ala Leu Ala Pro Tyr Ile Pro Ala 
1               5                   10                  15      


Asp Asp Asp Phe Gln Leu Arg Lys 
            20                  


<210>  14
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide incorporating iNOS degron

<400>  14

Lys Glu Glu Lys Asp Ile Asn Asn Asn Val Lys Lys Thr Lys 
1               5                   10                  


<210>  15
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide incorporating SRC3 degron

<400>  15

Asp Val Gln Lys Ala Asp Val Ser Ser Thr Gly Gln Gly Ile Asp Ser 
1               5                   10                  15      


Lys 
    


<210>  16
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide incorporating Cyclin D1 degron

<400>  16

Lys Ala Ala Glu Glu Glu Glu Ser Leu Ala Ser Thr Pro Thr Asp Val 
1               5                   10                  15      


Arg Asp Val Asp Ile Lys 
            20          


<210>  17
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide incorporating IFNAR1 degron

<400>  17

Lys Lys Tyr Ser Ser Gln Thr Ser Gln Asp Ser Gly Asn Tyr Ser Asn 
1               5                   10                  15      


Lys 
    


<210>  18
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide incorporating p53 degron

<400>  18

Lys Pro Leu Ser Ser Ser Val Pro Ser Gln Lys Thr Tyr Gln Gly Ser 
1               5                   10                  15      


Tyr Gly Phe Arg Leu Gly Lys 
            20              


<210>  19
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide incorporating beta catenin degron

<400>  19

Lys Ala Trp Gln Gln Gln Ser Tyr Leu Asp Ser Gly Ile His Ser Gly 
1               5                   10                  15      


Ala Thr Thr Thr Ala Pro Lys 
            20              


<210>  20
<211>  1077
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMD0185 plasmid ORF encoding for a control (no degron) 
       YFP-bearing construct

<400>  20
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagt      720

ggaggcatgt cggctaccgc tgccaccgtc ccgcctgccg ccccggccgg cgagggtggc      780

ccccctgcac ctcctccaaa tcttaccagt aacaggagac tgcagcagac ccaggcccag      840

gtggatgagg tggtggacat catgagggtg aatgtggaca aggtcctgga gcgrgaccag      900

aagctatcgg aactggatga tcgcgcagat gccctccagg caggggcctc ccagtttgaa      960

acaagtgcag ccaagctcaa gcgcaaatac tggtggaaaa acctcaagat gatgatcatc     1020

ttgggagtga tttgcgccat catcctcatc atcatcatcg tttacttcag cacttaa        1077


<210>  21
<211>  1077
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMD0185 ORF encoding for a control/common EFP-bearing construct

<400>  21
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac      480

ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagt      720

ggaggcatgt cggctaccgc tgccaccgtc ccgcctgccg ccccggccgg cgagggtggc      780

ccccctgcac ctcctccaaa tcttaccagt aacaggagac tgcagcagac ccaggcccag      840

gtggatgagg tggtggacat catgagggtg aatgtggaca aggtcctgga gcgrgaccag      900

aagctatcgg aactgaataa tcgcgcaaat gccctccagg caggggcctc cgtgtttgaa      960

acaagtgcag ccaagctcaa gcgcaaatac tggtggaaaa acctcaagat gatgatcatc     1020

ttgggagtga tttgcgccat catcctcatc atcatcatcg tttacttcag cacttaa        1077


<210>  22
<211>  1122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMD0189 ORF encoding for a YFP-bearing construct having a Bonger 
       degron sequence

<400>  22
atgggcaaac gtcgccgtgg taacaaatcc ggtggcaagc ttgcaatggt gagcaagggc       60

gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc      120

cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg      180

aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc      240

ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc      300

aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc      360

aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag      420

ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac      480

tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac      540

ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag      600

aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag      660

tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg      720

accgccgccg ggatcactct cggcatggac gagctgtaca agagtggagg catgtcggct      780

accgctgcca ccgtcccgcc tgccgccccg gccggcgagg gtggcccccc tgcacctcct      840

ccaaatctta ccagtaacag gagactgcag cagacccagg cccaggtgga tgaggtggtg      900

gacatcatga gggtgaatgt ggacaaggtc ctggagcgag accagaagct atcggaactg      960

gatgatcgcg cagatgccct ccaggcaggg gcctcccagt ttgaaacaag tgcagccaag     1020

ctcaagcgca aatactggtg gaaaaacctc aagatgatga tcatcttggg agtgatttgc     1080

gccatcatcc tcatcatcat catcgtttac ttcagcactt aa                        1122


<210>  23
<211>  1122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMD0190 ORF encoding for a YFP-bearing construct having an iNOS 
       degron sequence

<400>  23
atgggcaaag atattaataa caataaatcc ggtggcaagc ttgcaatggt gagcaagggc       60

gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc      120

cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg      180

aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc      240

ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc      300

aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc      360

aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag      420

ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac      480

tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac      540

ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag      600

aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag      660

tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg      720

accgccgccg ggatcactct cggcatggac gagctgtaca agagtggagg catgtcggct      780

accgctgcca ccgtcccgcc tgccgccccg gccggcgagg gtggcccccc tgcacctcct      840

ccaaatctta ccagtaacag gagactgcag cagacccagg cccaggtgga tgaggtggtg      900

gacatcatga gggtgaatgt ggacaaggtc ctggagcgag accagaagct atcggaactg      960

gatgatcgcg cagatgccct ccaggcaggg gcctcccagt ttgaaacaag tgcagccaag     1020

ctcaagcgca aatactggtg gaaaaacctc aagatgatga tcatcttggg agtgatttgc     1080

gccatcatcc tcatcatcat catcgtttac ttcagcactt aa                        1122


