               SEQUENCE LISTING

<110> National Research Council of Canada

<120> Winter Aconite Fatty Acid Elongase and Uses Thereof in the Production of Fatty Acids

<130> 2016-045-02

<150> US62/344,071
<151> 2016-06-01

<160> 7

<170> BiSSAP 1.3.6

<210> 1
<211> 26
<212> DNA
<213> Eranthis hyemalis


<220> 
<223> primer sequence

<400> 1
tctagaatgg agtccatttc tgctag                                         26


<210> 2
<211> 28
<212> DNA
<213> Eranthis hyemalis


<220> 
<223> primer sequence

<400> 2
tctagattaa accagcttct tatccttg                                       28


<210> 3
<211> 949
<212> DNA
<213> Eranthis hyemalis


<220> 
<223> Coding region 25...852

<400> 3
atgcttcctt catttgtttc aaaaatggag tccatttctg ctagtgtacg ctactggcta      60

gtagaacacc cattggtgag cggattcgag tggatagaag gcgaaacatt tggttcatcg     120

ccaaaatttc ttctaaccac ggtagccacc tacctctccc taacctacat cctctccatc     180

acccttcttt caccgaaacc tccagtgaaa accccctcca agacccttac catcctccgg     240

tctatctccg caatacataa cctgattctc cttgccctct ccttcataat ggccttggga     300

gcgacattag caaccaccac caaaatgcca agcaagcaat ggatctgttt cccagcaaac     360

aaaacccgat cacagggtcc actatttttc tgggcttatg tgttctacct atccaagata     420

cttgaatacg tagataccct cttgatcatc ctccacaacg acgcaaggag actcacattt     480

ctccatgtct accatcacac tgttgttact atcatgtgtt acctttggct acacactaca     540

caatctctct tacctttggg gattgttacc aatgccaccg tgcatactgt catgtatgct     600

tattatttca tgtgcacact tgggaaaagg ccatcttgga agaggttagt gacagatttc     660

cagatcattc agttttggtt tggtctcggg atctccacgt tgatgttgtg gttccatttt     720

actggaactg gctgctctgg gatttgggga tggggttttt cttatgtctt caatgcttct     780

cttcttgctc tatttagtgc ttttcatgct aacaactacg ccaacaagga caaggataag     840

aagctggttt aactgcctat ttatggggtc tattcgtgtg gctatatcac catcccacgc     900

gatcagaatc tatttaggat atccttgtat caataagtta agtttgttt                 949


<210> 4
<211> 275
<212> PRT
<213> Eranthis hyemalis


<400> 4
Met Glu Ser Ile Ser Ala Ser Val Arg Tyr Trp Leu Val Glu His Pro 
1               5                   10                  15      
Leu Val Ser Gly Phe Glu Trp Ile Glu Gly Glu Thr Phe Gly Ser Ser 
            20                  25                  30          
Pro Lys Phe Leu Leu Thr Thr Val Ala Thr Tyr Leu Ser Leu Thr Tyr 
        35                  40                  45              
Ile Leu Ser Ile Thr Leu Leu Ser Pro Lys Pro Pro Val Lys Thr Pro 
    50                  55                  60                  
Ser Lys Thr Leu Thr Ile Leu Arg Ser Ile Ser Ala Ile His Asn Leu 
65                  70                  75                  80  
Ile Leu Leu Ala Leu Ser Phe Ile Met Ala Leu Gly Ala Thr Leu Ala 
                85                  90                  95      
Thr Thr Thr Lys Met Pro Ser Lys Gln Trp Ile Cys Phe Pro Ala Asn 
            100                 105                 110         
Lys Thr Arg Ser Gln Gly Pro Leu Phe Phe Trp Ala Tyr Val Phe Tyr 
        115                 120                 125             
Leu Ser Lys Ile Leu Glu Tyr Val Asp Thr Leu Leu Ile Ile Leu His 
    130                 135                 140                 
Asn Asp Ala Arg Arg Leu Thr Phe Leu His Val Tyr His His Thr Val 
145                 150                 155                 160 
Val Thr Ile Met Cys Tyr Leu Trp Leu His Thr Thr Gln Ser Leu Leu 
                165                 170                 175     
Pro Leu Gly Ile Val Thr Asn Ala Thr Val His Thr Val Met Tyr Ala 
            180                 185                 190         
Tyr Tyr Phe Met Cys Thr Leu Gly Lys Arg Pro Ser Trp Lys Arg Leu 
        195                 200                 205             
Val Thr Asp Phe Gln Ile Ile Gln Phe Trp Phe Gly Leu Gly Ile Ser 
    210                 215                 220                 
Thr Leu Met Leu Trp Phe His Phe Thr Gly Thr Gly Cys Ser Gly Ile 
225                 230                 235                 240 
Trp Gly Trp Gly Phe Ser Tyr Val Phe Asn Ala Ser Leu Leu Ala Leu 
                245                 250                 255     
Phe Ser Ala Phe His Ala Asn Asn Tyr Ala Asn Lys Asp Lys Asp Lys 
            260                 265                 270         
Lys Leu Val 
        275 

<210> 5
<211> 828
<212> DNA
<213> Eranthis hyemalis


<400> 5
atggagtcca tttctgctag tgtacgctac tggctagtag aacacccatt ggtgagcgga      60

ttcgagtgga tagaaggcga aacatttggt tcatcgccaa aatttcttct aaccacggta     120

gccacctacc tctccctaac ctacatcctc tccatcaccc ttctttcacc gaaacctcca     180

gtgaaaaccc cctccaagac ccttaccatc ctccggtcta tctccgcaat acataacctg     240

attctccttg ccctctcctt cataatggcc ttgggagcga cattagcaac caccaccaaa     300

atgccaagca agcaatggat ctgtttccca gcaaacaaaa cccgatcaca gggtccacta     360

tttttctggg cttatgtgtt ctacctatcc aagatacttg aatacgtaga taccctcttg     420

atcatcctcc acaacgacgc aaggagactc acatttctcc atgtctacca tcacactgtt     480

gttactatca tgtgttacct ttggctacac actacacaat ctctcttacc tttggggatt     540

gttaccaatg ccaccgtgca tactgtcatg tatgcttatt atttcatgtg cacacttggg     600

aaaaggccat cttggaagag gttagtgaca gatttccaga tcattcagtt ttggtttggt     660

ctcgggatct ccacgttgat gttgtggttc cattttactg gaactggctg ctctgggatt     720

tggggatggg gtttttctta tgtcttcaat gcttctcttc ttgctctatt tagtgctttt     780

catgctaaca actacgccaa caaggacaag gataagaagc tggtttaa                  828


<210> 6
<211> 829
<212> DNA
<213> Artificial Sequence


<220> 
<223> Codon-Optimized EhEL01 coding sequence

<400> 6
atggagtcca tctctgcaag cgtccgttat tggcttgtag agcacccact tgtgtcagga      60

ttcgagtgga tcgagggaga gacttttggt tcttctccaa aatttttgct gaccactgtg     120

gctacttatc tatcgttaac gtatattctg tccatcactc ttctctctcc taaaccgcct     180

gtcaaaacac cgtctaagac tcttacgatc ttaagatcta ttagcgctat tcacaacttg     240

atcttgttgg ctcttagttt tatcatggca cttggagcaa cattggcgac aactaccaag     300

atgcccagca agcaatggat ctgtttcccg gctaacaaga ccaggagcca gggtccattg     360

ttcttctggg catacgtttt ttatctaagt aaaatcctgg aatacgtcga taccctcctt     420

ataatcctcc acaacgacgc gaggagacta acttttttgc atgtgtatca ccacactgtg     480

gttaccatca tgtgttattt gtggcttcat actacccaat cacttttgcc cttaggaata     540

gttacaaacg ccacagtgca taccgtaatg tacgcttact acttcatgtg taccctggga     600

aaacgtccat cttggaagag actagtcaca gatttccaaa ttatccaatt ctggtttggt     660

ctcgggatct cgacccttat gctctggttt cacttcacag gcactggttg tagcggaatc     720

tggggttggg gattttcata cgtctttaac gcttccttgt tggctctatt cagtgctttc     780

catgcaaaca actacgccaa caaggacaag gataagaagc tagtctgat                 829


<210> 7
<211> 990
<212> DNA
<213> Conidiobolus thromboides

<400> 7
atgagtttat taaatacatt ggatactatt acttcaagca ataatgttgt atcagcatac      60

aacgatgccc cagtagacta tttaattaaa gtagtagatt tagctttaac tgctaacaaa     120

gcagtcttca atgttataga agccaaagtt aacgtatgga tgccaacatt gatgataaac     180

ttaagagaac aggtctctaa tttaatctca ccaataagta aatacttgcc attgttagat     240

cctatcgaag tgttttctat cttgttttta tatatctttg ttgtgttttt ttggctcaaa     300

gtagcttcta gcttcctccc acgtttcgaa gtaagattat tttccctttt ccataatttc     360

tgtatggtcg ttttatccgc ctatatgtgc tcttccatcc tattacaagc ttatgcagat     420

aagtatattc tattcactaa ccccgtcgat cactctccaa atggtattcc aatggctaaa     480

ataatatggt tattttatat ttccaaaatc ccagagttcg ttgacactat gatcatgttg     540

gttaaacaaa actaccgcca aatctccttt ttacatgtct accatcatag ttcgatcttt     600

gctatttggt ggattgttac cttgatggca ccaaatggtg atgcttattt ctcagctgca     660

ttgaactcat ttattcatgt tgttatgtac ggatattatt tactctctgc acttggattc     720

aaatccgtct cctttgttaa gaaatatatt actatgggac aaatgactca atttgcactc     780

aactttgttc aagctagtta taatattgta gacagaaatt acttacgtcc acaagtccat     840

gagcaaggat tagcctatcc ttatgctctt tccgttttac tttggttcta tatgatctct     900

atgttggtgt tattcgctaa cttttatatt caagatcgta tccgtcaatc aaagttaaag     960

tctcaacaaa agggaaagaa aatgaattag                                      990


