                         SEQUENCE LISTING

<110>  Alma Mater Studiorum Universita di Bologna
 
<120>  Herpesvirus with modified glycoprotein D

<130>  U70628PC

<150>  EP 16173830.7
<151>  2016-06-09

<150>  EP 17000247.1
<151>  2017-02-15

<160>  53    

<170>  PatentIn version 3.5

<210>  1
<211>  394
<212>  PRT
<213>  Herpes simplex virus 1

<400>  1

Met Gly Gly Ala Ala Ala Arg Leu Gly Ala Val Ile Leu Phe Val Val 
1               5                   10                  15      


Ile Val Gly Leu His Gly Val Arg Gly Lys Tyr Ala Leu Ala Asp Ala 
            20                  25                  30          


Ser Leu Lys Met Ala Asp Pro Asn Arg Phe Arg Gly Lys Asp Leu Pro 
        35                  40                  45              


Val Leu Asp Gln Leu Thr Asp Pro Pro Gly Val Arg Arg Val Tyr His 
    50                  55                  60                  


Ile Gln Ala Gly Leu Pro Asp Pro Phe Gln Pro Pro Ser Leu Pro Ile 
65                  70                  75                  80  


Thr Val Tyr Tyr Ala Val Leu Glu Arg Ala Cys Arg Ser Val Leu Leu 
                85                  90                  95      


Asn Ala Pro Ser Glu Ala Pro Gln Ile Val Arg Gly Ala Ser Glu Asp 
            100                 105                 110         


Val Arg Lys Gln Pro Tyr Asn Leu Thr Ile Ala Trp Phe Arg Met Gly 
        115                 120                 125             


Gly Asn Cys Ala Ile Pro Ile Thr Val Met Glu Tyr Thr Glu Cys Ser 
    130                 135                 140                 


Tyr Asn Lys Ser Leu Gly Ala Cys Pro Ile Arg Thr Gln Pro Arg Trp 
145                 150                 155                 160 


Asn Tyr Tyr Asp Ser Phe Ser Ala Val Ser Glu Asp Asn Leu Gly Phe 
                165                 170                 175     


Leu Met His Ala Pro Ala Phe Glu Thr Ala Gly Thr Tyr Leu Arg Leu 
            180                 185                 190         


Val Lys Ile Asn Asp Trp Thr Glu Ile Thr Gln Phe Ile Leu Glu His 
        195                 200                 205             


Arg Ala Lys Gly Ser Cys Lys Tyr Ala Leu Pro Leu Arg Ile Pro Pro 
    210                 215                 220                 


Ser Ala Cys Leu Ser Pro Gln Ala Tyr Gln Gln Gly Val Thr Val Asp 
225                 230                 235                 240 


Ser Ile Gly Met Leu Pro Arg Phe Ile Pro Glu Asn Gln Arg Thr Val 
                245                 250                 255     


Ala Val Tyr Ser Leu Lys Ile Ala Gly Trp His Gly Pro Lys Ala Pro 
            260                 265                 270         


Tyr Thr Ser Thr Leu Leu Pro Pro Glu Leu Ser Glu Thr Pro Asn Ala 
        275                 280                 285             


Thr Gln Pro Glu Leu Ala Pro Glu Asp Pro Glu Asp Ser Ala Leu Leu 
    290                 295                 300                 


Glu Asp Pro Val Gly Thr Val Ala Pro Gln Ile Pro Pro Asn Trp His 
305                 310                 315                 320 


Ile Pro Ser Ile Gln Asp Ala Ala Thr Pro Tyr His Pro Pro Ala Thr 
                325                 330                 335     


Pro Asn Asn Met Gly Leu Ile Ala Gly Ala Val Gly Gly Ser Leu Leu 
            340                 345                 350         


Ala Ala Leu Val Ile Cys Gly Ile Val Tyr Trp Met Arg Arg Arg Thr 
        355                 360                 365             


Gln Lys Ala Pro Lys Arg Ile Arg Leu Pro His Ile Arg Glu Asp Asp 
    370                 375                 380                 


Gln Pro Ser Ser His Gln Pro Leu Phe Tyr 
385                 390                 


<210>  2
<211>  2010
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence of chimeric gD-GCN4, scFv HER2 of R-87

<400>  2
atgggggggg ctgccgccag gttgggggcc gtgattttgt ttgtcgtcat agtgggcctc       60

catggggtcc gcggcaaata tgccttggcg gatgcctctc tcaagatggc cgaccccaat      120

cgctttcgcg gcaaagacct tccggtcgga tccaagaact accacctgga gaacgaggtg      180

gccagactga agaagctggt gggcagcctg gaccagctga ccgaccctcc gggggtcgag      240

aattccgata tccagatgac ccagtccccg agctccctgt ccgcctctgt gggcgatagg      300

gtcaccatca cctgccgtgc cagtcaggat gtgaatactg ctgtagcctg gtatcaacag      360

aaaccaggaa aagctccgaa gcttctgatt tactcggcat ccttcctcta ctctggagtc      420

ccttctcgct tctctggtag ccgttccggg acggatttca ctctgaccat cagcagtctg      480

cagccggaag acttcgcaac ttattactgt cagcaacatt atactactcc tcccacgttc      540

ggacagggta ccaaggtgga gatcaaatcg gatatgccga tggctgatcc gaaccgtttc      600

cgcggtaaga acctggtttt tcattctgag gttcagctgg tggagtctgg cggtggcctg      660

gtgcagccag ggggctcact ccgtttgtcc tgtgcagctt ctggcttcaa cattaaagac      720

acctatatac actgggtgcg tcaggccccg ggtaagggcc tggaatgggt tgcaaggatt      780

tatcctacga atggttatac tagatatgcc gatagcgtca agggccgttt cactataagc      840

gcagacacat ccaaaaacac agcctaccta caaatgaaca gcttaagagc tgaggacact      900

gccgtctatt attgtagccg ctggggaggg gacggcttct atgctatgga ctactggggt      960

caaggaacac tagtcaccgt ctcctcgagt ggcggtggct ctggttccgg tggatccatc     1020

caggcgggcc taccagaccc gttccagccc cccagcctcc cgatcacggt ttactacgcc     1080

gtgttggagc gcgcctgccg cagcgtgctc ctaaacgcac cgtcggaggc cccccagatt     1140

gtccgcgggg cctccgaaga cgtccggaaa caaccctaca acctgaccat cgcttggttt     1200

cggatgggag gcaactgtgc tatccccatc acggtcatgg agtacaccga atgctcctac     1260

aacaagtctc tgggggcctg tcccatccga acgcagcccc gctggaacta ctatgacagc     1320

ttcagcgccg tcagcgagga taacctgggg ttcctgatgc acgcccccgc gtttgagacc     1380

gccggcacgt acctgcggct cgtgaagata aacgactgga cggagattac acagtttatc     1440

ctggagcacc gagccaaggg ctcctgtaag tacgccctcc cgctgcgcat ccccccgtca     1500

gcctgcctgt ccccccaggc ctaccagcag ggggtgacgg tggacagcat cgggatgctg     1560

ccccgcttca tccccgagaa ccagcgcacc gtcgccgtat acagcttgaa gatcgccggg     1620

tggcacgggc ccaaggcccc atacacgagc accctgctgc ccccggagct gtccgagacc     1680

cccaacgcca cgcagccaga actcgccccg gaagaccccg aggattcggc cctcttggag     1740

gaccccgtgg ggacggtggc gccgcaaatc ccaccaaact ggcacatacc gtcgatccag     1800

gacgccgcga cgccttacca tcccccggcc accccgaaca acatgggcct gatcgccggc     1860

gcggtgggcg gcagtctcct ggcagccctg gtcatttgcg gaattgtgta ctggatgcgc     1920

cgccgcactc aaaaagcccc aaagcgcata cgcctccccc acatccggga agacgaccag     1980

ccgtcctcgc accagccctt gttttactag                                      2010


<210>  3
<211>  669
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the precursor of gD having inserted the 
       GCN4 peptide and scFv to HER2 receptor as encoded by the 
       construct R-87.

<400>  3

Met Gly Gly Ala Ala Ala Arg Leu Gly Ala Val Ile Leu Phe Val Val 
1               5                   10                  15      


Ile Val Gly Leu His Gly Val Arg Gly Lys Tyr Ala Leu Ala Asp Ala 
            20                  25                  30          


Ser Leu Lys Met Ala Asp Pro Asn Arg Phe Arg Gly Lys Asp Leu Pro 
        35                  40                  45              


Val Gly Ser Lys Asn Tyr His Leu Glu Asn Glu Val Ala Arg Leu Lys 
    50                  55                  60                  


Lys Leu Val Gly Ser Leu Asp Gln Leu Thr Asp Pro Pro Gly Val Glu 
65                  70                  75                  80  


Asn Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
                85                  90                  95      


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn 
            100                 105                 110         


Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
        115                 120                 125             


Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe 
    130                 135                 140                 


Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
145                 150                 155                 160 


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr 
                165                 170                 175     


Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser Asp Met 
            180                 185                 190         


Pro Met Ala Asp Pro Asn Arg Phe Arg Gly Lys Asn Leu Val Phe His 
        195                 200                 205             


Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
    210                 215                 220                 


Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp 
225                 230                 235                 240 


Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
                245                 250                 255     


Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser 
            260                 265                 270         


Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala 
        275                 280                 285             


Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr 
    290                 295                 300                 


Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly 
305                 310                 315                 320 


Gln Gly Thr Leu Val Thr Val Ser Ser Ser Gly Gly Gly Ser Gly Ser 
                325                 330                 335     


Gly Gly Ser Ile Gln Ala Gly Leu Pro Asp Pro Phe Gln Pro Pro Ser 
            340                 345                 350         


Leu Pro Ile Thr Val Tyr Tyr Ala Val Leu Glu Arg Ala Cys Arg Ser 
        355                 360                 365             


Val Leu Leu Asn Ala Pro Ser Glu Ala Pro Gln Ile Val Arg Gly Ala 
    370                 375                 380                 


Ser Glu Asp Val Arg Lys Gln Pro Tyr Asn Leu Thr Ile Ala Trp Phe 
385                 390                 395                 400 


Arg Met Gly Gly Asn Cys Ala Ile Pro Ile Thr Val Met Glu Tyr Thr 
                405                 410                 415     


Glu Cys Ser Tyr Asn Lys Ser Leu Gly Ala Cys Pro Ile Arg Thr Gln 
            420                 425                 430         


Pro Arg Trp Asn Tyr Tyr Asp Ser Phe Ser Ala Val Ser Glu Asp Asn 
        435                 440                 445             


Leu Gly Phe Leu Met His Ala Pro Ala Phe Glu Thr Ala Gly Thr Tyr 
    450                 455                 460                 


Leu Arg Leu Val Lys Ile Asn Asp Trp Thr Glu Ile Thr Gln Phe Ile 
465                 470                 475                 480 


Leu Glu His Arg Ala Lys Gly Ser Cys Lys Tyr Ala Leu Pro Leu Arg 
                485                 490                 495     


Ile Pro Pro Ser Ala Cys Leu Ser Pro Gln Ala Tyr Gln Gln Gly Val 
            500                 505                 510         


Thr Val Asp Ser Ile Gly Met Leu Pro Arg Phe Ile Pro Glu Asn Gln 
        515                 520                 525             


Arg Thr Val Ala Val Tyr Ser Leu Lys Ile Ala Gly Trp His Gly Pro 
    530                 535                 540                 


Lys Ala Pro Tyr Thr Ser Thr Leu Leu Pro Pro Glu Leu Ser Glu Thr 
545                 550                 555                 560 


Pro Asn Ala Thr Gln Pro Glu Leu Ala Pro Glu Asp Pro Glu Asp Ser 
                565                 570                 575     


Ala Leu Leu Glu Asp Pro Val Gly Thr Val Ala Pro Gln Ile Pro Pro 
            580                 585                 590         


Asn Trp His Ile Pro Ser Ile Gln Asp Ala Ala Thr Pro Tyr His Pro 
        595                 600                 605             


Pro Ala Thr Pro Asn Asn Met Gly Leu Ile Ala Gly Ala Val Gly Gly 
    610                 615                 620                 


Ser Leu Leu Ala Ala Leu Val Ile Cys Gly Ile Val Tyr Trp Met Arg 
625                 630                 635                 640 


Arg Arg Thr Gln Lys Ala Pro Lys Arg Ile Arg Leu Pro His Ile Arg 
                645                 650                 655     


Glu Asp Asp Gln Pro Ser Ser His Gln Pro Leu Phe Tyr 
            660                 665                 


<210>  4
<211>  1995
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence of chimeric gD-GCN4, scFv HER2 of R-89

<400>  4
atgggggggg ctgccgccag gttgggggcc gtgattttgt ttgtcgtcat agtgggcctc       60

catggggtcc gcggcaaata tgccttggcg gatgcctctc tcaagatggc cgaccccaat      120

cgctttcgcg gcaaagacct tccggtcgga tccaagaact accacctgga gaacgaggtg      180

gccagactga agaagctggt gggcagcctg gaccagctga ccgaccctcc gggggtccgg      240

cgcgtgtacc acatccaggc gggcctacca gacccgttcc agccccccag cctcccgatc      300

acggtttact acgccgtgtt ggagcgcgcc tgccgcagcg tgctcctaaa cgcaccgtcg      360

gaggcccccc agattgtccg cggggcctcc gaagacgtcc ggaaacaacc ctacaacctg      420

accatcgctt ggtttcggat gggaggcaac tgtgctatcc ccatcacggt catggagtac      480

accgaatgct cctacaacaa gtctctgggg gcctgtccca tccgaacgca gccccgctgg      540

aactactatg acagcttcag cgccgtcagc gaggataacc tggggttcct gatgcacgcc      600

cccgcgtttg agaccgccgg cacgtacctg cggctcgtga agataaacga ctggacggag      660

attacacagt ttatcctgga gcaccgagcc aagggctcct gtaagtacgc cctcccgctg      720

cgcatccccc cgtcagcctg cctgtccccc caggcctacc agcagggggt gacggtggac      780

agcatcggga tgctgccccg cttcgagaat tccgatatcc agatgaccca gtccccgagc      840

tccctgtccg cctctgtggg cgatagggtc accatcacct gccgtgccag tcaggatgtg      900

aatactgctg tagcctggta tcaacagaaa ccaggaaaag ctccgaagct tctgatttac      960

tcggcatcct tcctctactc tggagtccct tctcgcttct ctggtagccg ttccgggacg     1020

gatttcactc tgaccatcag cagtctgcag ccggaagact tcgcaactta ttactgtcag     1080

caacattata ctactcctcc cacgttcgga cagggtacca aggtggagat caaatcggat     1140

atgccgatgg ctgatccgaa ccgtttccgc ggtaagaacc tggtttttca ttctgaggtt     1200

cagctggtgg agtctggcgg tggcctggtg cagccagggg gctcactccg tttgtcctgt     1260

gcagcttctg gcttcaacat taaagacacc tatatacact gggtgcgtca ggccccgggt     1320

aagggcctgg aatgggttgc aaggatttat cctacgaatg gttatactag atatgccgat     1380

agcgtcaagg gccgtttcac tataagcgca gacacatcca aaaacacagc ctacctacaa     1440

atgaacagct taagagctga ggacactgcc gtctattatt gtagccgctg gggaggggac     1500

ggcttctatg ctatggacta ctggggtcaa ggaacactag tcaccgtctc ctcgagtggc     1560

ggtggctctg gttccggtgg atcctacagc ttgaagatcg ccgggtggca cgggcccaag     1620

gccccataca cgagcaccct gctgcccccg gagctgtccg agacccccaa cgccacgcag     1680

ccagaactcg ccccggaaga ccccgaggat tcggccctct tggaggaccc cgtggggacg     1740

gtggcgccgc aaatcccacc aaactggcac ataccgtcga tccaggacgc cgcgacgcct     1800

taccatcccc cggccacccc gaacaacatg ggcctgatcg ccggcgcggt gggcggcagt     1860

ctcctggcag ccctggtcat ttgcggaatt gtgtactgga tgcgccgccg cactcaaaaa     1920

gccccaaagc gcatacgcct cccccacatc cgggaagacg accagccgtc ctcgcaccag     1980

cccttgtttt actag                                                      1995


<210>  5
<211>  664
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the precursor of gD  having inserted the 
       GCN4 peptide  and scFv to HER2 receptor as encoded by the 
       construct R-89.

<400>  5

Met Gly Gly Ala Ala Ala Arg Leu Gly Ala Val Ile Leu Phe Val Val 
1               5                   10                  15      


Ile Val Gly Leu His Gly Val Arg Gly Lys Tyr Ala Leu Ala Asp Ala 
            20                  25                  30          


Ser Leu Lys Met Ala Asp Pro Asn Arg Phe Arg Gly Lys Asp Leu Pro 
        35                  40                  45              


Val Gly Ser Lys Asn Tyr His Leu Glu Asn Glu Val Ala Arg Leu Lys 
    50                  55                  60                  


Lys Leu Val Gly Ser Leu Asp Gln Leu Thr Asp Pro Pro Gly Val Arg 
65                  70                  75                  80  


Arg Val Tyr His Ile Gln Ala Gly Leu Pro Asp Pro Phe Gln Pro Pro 
                85                  90                  95      


Ser Leu Pro Ile Thr Val Tyr Tyr Ala Val Leu Glu Arg Ala Cys Arg 
            100                 105                 110         


Ser Val Leu Leu Asn Ala Pro Ser Glu Ala Pro Gln Ile Val Arg Gly 
        115                 120                 125             


Ala Ser Glu Asp Val Arg Lys Gln Pro Tyr Asn Leu Thr Ile Ala Trp 
    130                 135                 140                 


Phe Arg Met Gly Gly Asn Cys Ala Ile Pro Ile Thr Val Met Glu Tyr 
145                 150                 155                 160 


Thr Glu Cys Ser Tyr Asn Lys Ser Leu Gly Ala Cys Pro Ile Arg Thr 
                165                 170                 175     


Gln Pro Arg Trp Asn Tyr Tyr Asp Ser Phe Ser Ala Val Ser Glu Asp 
            180                 185                 190         


Asn Leu Gly Phe Leu Met His Ala Pro Ala Phe Glu Thr Ala Gly Thr 
        195                 200                 205             


Tyr Leu Arg Leu Val Lys Ile Asn Asp Trp Thr Glu Ile Thr Gln Phe 
    210                 215                 220                 


Ile Leu Glu His Arg Ala Lys Gly Ser Cys Lys Tyr Ala Leu Pro Leu 
225                 230                 235                 240 


Arg Ile Pro Pro Ser Ala Cys Leu Ser Pro Gln Ala Tyr Gln Gln Gly 
                245                 250                 255     


Val Thr Val Asp Ser Ile Gly Met Leu Pro Arg Phe Glu Asn Ser Asp 
            260                 265                 270         


Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp 
        275                 280                 285             


Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala Val 
    290                 295                 300                 


Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr 
305                 310                 315                 320 


Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
                325                 330                 335     


Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu 
            340                 345                 350         


Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro Thr 
        355                 360                 365             


Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser Asp Met Pro Met Ala 
    370                 375                 380                 


Asp Pro Asn Arg Phe Arg Gly Lys Asn Leu Val Phe His Ser Glu Val 
385                 390                 395                 400 


Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu 
                405                 410                 415     


Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr Tyr Ile 
            420                 425                 430         


His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg 
        435                 440                 445             


Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val Lys Gly 
    450                 455                 460                 


Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu Gln 
465                 470                 475                 480 


Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ser Arg 
                485                 490                 495     


Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr 
            500                 505                 510         


Leu Val Thr Val Ser Ser Ser Gly Gly Gly Ser Gly Ser Gly Gly Ser 
        515                 520                 525             


Tyr Ser Leu Lys Ile Ala Gly Trp His Gly Pro Lys Ala Pro Tyr Thr 
    530                 535                 540                 


Ser Thr Leu Leu Pro Pro Glu Leu Ser Glu Thr Pro Asn Ala Thr Gln 
545                 550                 555                 560 


Pro Glu Leu Ala Pro Glu Asp Pro Glu Asp Ser Ala Leu Leu Glu Asp 
                565                 570                 575     


Pro Val Gly Thr Val Ala Pro Gln Ile Pro Pro Asn Trp His Ile Pro 
            580                 585                 590         


Ser Ile Gln Asp Ala Ala Thr Pro Tyr His Pro Pro Ala Thr Pro Asn 
        595                 600                 605             


Asn Met Gly Leu Ile Ala Gly Ala Val Gly Gly Ser Leu Leu Ala Ala 
    610                 615                 620                 


Leu Val Ile Cys Gly Ile Val Tyr Trp Met Arg Arg Arg Thr Gln Lys 
625                 630                 635                 640 


Ala Pro Lys Arg Ile Arg Leu Pro His Ile Arg Glu Asp Asp Gln Pro 
                645                 650                 655     


Ser Ser His Gln Pro Leu Phe Tyr 
            660                 


<210>  6
<211>  2010
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence of chimeric gD-GCN4, scFv HER2 of R-97

<400>  6
atgggggggg ctgccgccag gttgggggcc gtgattttgt ttgtcgtcat agtgggcctc       60

catggggtcc gcggcaaata tgccttggcg gatgcctctc tcaagatggc cgaccccaat      120

cgctttcgcg gcaaagacct tccggtcgag aattccgata tccagatgac ccagtccccg      180

agctccctgt ccgcctctgt gggcgatagg gtcaccatca cctgccgtgc cagtcaggat      240

gtgaatactg ctgtagcctg gtatcaacag aaaccaggaa aagctccgaa gcttctgatt      300

tactcggcat ccttcctcta ctctggagtc ccttctcgct tctctggtag ccgttccggg      360

acggatttca ctctgaccat cagcagtctg cagccggaag acttcgcaac ttattactgt      420

cagcaacatt atactactcc tcccacgttc ggacagggta ccaaggtgga gatcaaatcg      480

gatatgccga tggctgatcc gaaccgtttc cgcggtaaga acctggtttt tcattctgag      540

gttcagctgg tggagtctgg cggtggcctg gtgcagccag ggggctcact ccgtttgtcc      600

tgtgcagctt ctggcttcaa cattaaagac acctatatac actgggtgcg tcaggccccg      660

ggtaagggcc tggaatgggt tgcaaggatt tatcctacga atggttatac tagatatgcc      720

gatagcgtca agggccgttt cactataagc gcagacacat ccaaaaacac agcctaccta      780

caaatgaaca gcttaagagc tgaggacact gccgtctatt attgtagccg ctggggaggg      840

gacggcttct atgctatgga ctactggggt caaggaacac tagtcaccgt ctcctcgagt      900

ggcggtggct ctggttccgg tggatccctg gaccagctga ccgaccctcc gggggtcgga      960

tccaagaact accacctgga gaacgaggtg gccagactga agaagctggt gggcagcatc     1020

caggcgggcc taccagaccc gttccagccc cccagcctcc cgatcacggt ttactacgcc     1080

gtgttggagc gcgcctgccg cagcgtgctc ctaaacgcac cgtcggaggc cccccagatt     1140

gtccgcgggg cctccgaaga cgtccggaaa caaccctaca acctgaccat cgcttggttt     1200

cggatgggag gcaactgtgc tatccccatc acggtcatgg agtacaccga atgctcctac     1260

aacaagtctc tgggggcctg tcccatccga acgcagcccc gctggaacta ctatgacagc     1320

ttcagcgccg tcagcgagga taacctgggg ttcctgatgc acgcccccgc gtttgagacc     1380

gccggcacgt acctgcggct cgtgaagata aacgactgga cggagattac acagtttatc     1440

ctggagcacc gagccaaggg ctcctgtaag tacgccctcc cgctgcgcat ccccccgtca     1500

gcctgcctgt ccccccaggc ctaccagcag ggggtgacgg tggacagcat cgggatgctg     1560

ccccgcttca tccccgagaa ccagcgcacc gtcgccgtat acagcttgaa gatcgccggg     1620

tggcacgggc ccaaggcccc atacacgagc accctgctgc ccccggagct gtccgagacc     1680

cccaacgcca cgcagccaga actcgccccg gaagaccccg aggattcggc cctcttggag     1740

gaccccgtgg ggacggtggc gccgcaaatc ccaccaaact ggcacatacc gtcgatccag     1800

gacgccgcga cgccttacca tcccccggcc accccgaaca acatgggcct gatcgccggc     1860

gcggtgggcg gcagtctcct ggcagccctg gtcatttgcg gaattgtgta ctggatgcgc     1920

cgccgcactc aaaaagcccc aaagcgcata cgcctccccc acatccggga agacgaccag     1980

ccgtcctcgc accagccctt gttttactag                                      2010


<210>  7
<211>  669
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the precursor of gD having inserted the 
       scFv to HER2 receptor and the GCN4 peptide as encoded by the 
       construct R-97

<400>  7

Met Gly Gly Ala Ala Ala Arg Leu Gly Ala Val Ile Leu Phe Val Val 
1               5                   10                  15      


Ile Val Gly Leu His Gly Val Arg Gly Lys Tyr Ala Leu Ala Asp Ala 
            20                  25                  30          


Ser Leu Lys Met Ala Asp Pro Asn Arg Phe Arg Gly Lys Asp Leu Pro 
        35                  40                  45              


Val Glu Asn Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
    50                  55                  60                  


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp 
65                  70                  75                  80  


Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
                85                  90                  95      


Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser 
            100                 105                 110         


Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
        115                 120                 125             


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr 
    130                 135                 140                 


Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser 
145                 150                 155                 160 


Asp Met Pro Met Ala Asp Pro Asn Arg Phe Arg Gly Lys Asn Leu Val 
                165                 170                 175     


Phe His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            180                 185                 190         


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile 
        195                 200                 205             


Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    210                 215                 220                 


Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala 
225                 230                 235                 240 


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn 
                245                 250                 255     


Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            260                 265                 270         


Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr 
        275                 280                 285             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ser Gly Gly Gly Ser 
    290                 295                 300                 


Gly Ser Gly Gly Ser Leu Asp Gln Leu Thr Asp Pro Pro Gly Val Gly 
305                 310                 315                 320 


Ser Lys Asn Tyr His Leu Glu Asn Glu Val Ala Arg Leu Lys Lys Leu 
                325                 330                 335     


Val Gly Ser Ile Gln Ala Gly Leu Pro Asp Pro Phe Gln Pro Pro Ser 
            340                 345                 350         


Leu Pro Ile Thr Val Tyr Tyr Ala Val Leu Glu Arg Ala Cys Arg Ser 
        355                 360                 365             


Val Leu Leu Asn Ala Pro Ser Glu Ala Pro Gln Ile Val Arg Gly Ala 
    370                 375                 380                 


Ser Glu Asp Val Arg Lys Gln Pro Tyr Asn Leu Thr Ile Ala Trp Phe 
385                 390                 395                 400 


Arg Met Gly Gly Asn Cys Ala Ile Pro Ile Thr Val Met Glu Tyr Thr 
                405                 410                 415     


Glu Cys Ser Tyr Asn Lys Ser Leu Gly Ala Cys Pro Ile Arg Thr Gln 
            420                 425                 430         


Pro Arg Trp Asn Tyr Tyr Asp Ser Phe Ser Ala Val Ser Glu Asp Asn 
        435                 440                 445             


Leu Gly Phe Leu Met His Ala Pro Ala Phe Glu Thr Ala Gly Thr Tyr 
    450                 455                 460                 


Leu Arg Leu Val Lys Ile Asn Asp Trp Thr Glu Ile Thr Gln Phe Ile 
465                 470                 475                 480 


Leu Glu His Arg Ala Lys Gly Ser Cys Lys Tyr Ala Leu Pro Leu Arg 
                485                 490                 495     


Ile Pro Pro Ser Ala Cys Leu Ser Pro Gln Ala Tyr Gln Gln Gly Val 
            500                 505                 510         


Thr Val Asp Ser Ile Gly Met Leu Pro Arg Phe Ile Pro Glu Asn Gln 
        515                 520                 525             


Arg Thr Val Ala Val Tyr Ser Leu Lys Ile Ala Gly Trp His Gly Pro 
    530                 535                 540                 


Lys Ala Pro Tyr Thr Ser Thr Leu Leu Pro Pro Glu Leu Ser Glu Thr 
545                 550                 555                 560 


Pro Asn Ala Thr Gln Pro Glu Leu Ala Pro Glu Asp Pro Glu Asp Ser 
                565                 570                 575     


Ala Leu Leu Glu Asp Pro Val Gly Thr Val Ala Pro Gln Ile Pro Pro 
            580                 585                 590         


Asn Trp His Ile Pro Ser Ile Gln Asp Ala Ala Thr Pro Tyr His Pro 
        595                 600                 605             


Pro Ala Thr Pro Asn Asn Met Gly Leu Ile Ala Gly Ala Val Gly Gly 
    610                 615                 620                 


Ser Leu Leu Ala Ala Leu Val Ile Cys Gly Ile Val Tyr Trp Met Arg 
625                 630                 635                 640 


Arg Arg Thr Gln Lys Ala Pro Lys Arg Ile Arg Leu Pro His Ile Arg 
                645                 650                 655     


Glu Asp Asp Gln Pro Ser Ser His Gln Pro Leu Phe Tyr 
            660                 665                 


<210>  8
<211>  1995
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence of chimeric gD-GCN4, scFv HER2 of R-99

<400>  8
atgggggggg ctgccgccag gttgggggcc gtgattttgt ttgtcgtcat agtgggcctc       60

catggggtcc gcggcaaata tgccttggcg gatgcctctc tcaagatggc cgaccccaat      120

cgctttcgcg gcaaagacct tccggtcgag aattccgata tccagatgac ccagtccccg      180

agctccctgt ccgcctctgt gggcgatagg gtcaccatca cctgccgtgc cagtcaggat      240

gtgaatactg ctgtagcctg gtatcaacag aaaccaggaa aagctccgaa gcttctgatt      300

tactcggcat ccttcctcta ctctggagtc ccttctcgct tctctggtag ccgttccggg      360

acggatttca ctctgaccat cagcagtctg cagccggaag acttcgcaac ttattactgt      420

cagcaacatt atactactcc tcccacgttc ggacagggta ccaaggtgga gatcaaatcg      480

gatatgccga tggctgatcc gaaccgtttc cgcggtaaga acctggtttt tcattctgag      540

gttcagctgg tggagtctgg cggtggcctg gtgcagccag ggggctcact ccgtttgtcc      600

tgtgcagctt ctggcttcaa cattaaagac acctatatac actgggtgcg tcaggccccg      660

ggtaagggcc tggaatgggt tgcaaggatt tatcctacga atggttatac tagatatgcc      720

gatagcgtca agggccgttt cactataagc gcagacacat ccaaaaacac agcctaccta      780

caaatgaaca gcttaagagc tgaggacact gccgtctatt attgtagccg ctggggaggg      840

gacggcttct atgctatgga ctactggggt caaggaacac tagtcaccgt ctcctcgagt      900

ggcggtggct ctggttccgg tggatccctg gaccagctga ccgaccctcc gggggtccgg      960

cgcgtgtacc acatccaggc gggcctacca gacccgttcc agccccccag cctcccgatc     1020

acggtttact acgccgtgtt ggagcgcgcc tgccgcagcg tgctcctaaa cgcaccgtcg     1080

gaggcccccc agattgtccg cggggcctcc gaagacgtcc ggaaacaacc ctacaacctg     1140

accatcgctt ggtttcggat gggaggcaac tgtgctatcc ccatcacggt catggagtac     1200

accgaatgct cctacaacaa gtctctgggg gcctgtccca tccgaacgca gccccgctgg     1260

aactactatg acagcttcag cgccgtcagc gaggataacc tggggttcct gatgcacgcc     1320

cccgcgtttg agaccgccgg cacgtacctg cggctcgtga agataaacga ctggacggag     1380

attacacagt ttatcctgga gcaccgagcc aagggctcct gtaagtacgc cctcccgctg     1440

cgcatccccc cgtcagcctg cctgtccccc caggcctacc agcagggggt gacggtggac     1500

agcatcggga tgctgccccg cttcggatcc aagaactacc acctggagaa cgaggtggcc     1560

agactgaaga agctggtggg cagctacagc ttgaagatcg ccgggtggca cgggcccaag     1620

gccccataca cgagcaccct gctgcccccg gagctgtccg agacccccaa cgccacgcag     1680

ccagaactcg ccccggaaga ccccgaggat tcggccctct tggaggaccc cgtggggacg     1740

gtggcgccgc aaatcccacc aaactggcac ataccgtcga tccaggacgc cgcgacgcct     1800

taccatcccc cggccacccc gaacaacatg ggcctgatcg ccggcgcggt gggcggcagt     1860

ctcctggcag ccctggtcat ttgcggaatt gtgtactgga tgcgccgccg cactcaaaaa     1920

gccccaaagc gcatacgcct cccccacatc cgggaagacg accagccgtc ctcgcaccag     1980

cccttgtttt actag                                                      1995


<210>  9
<211>  664
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the precursor of gD having inserted the 
       scFv to HER2 receptor and the GCN4 peptide as encoded by the 
       construct R-99.

<400>  9

Met Gly Gly Ala Ala Ala Arg Leu Gly Ala Val Ile Leu Phe Val Val 
1               5                   10                  15      


Ile Val Gly Leu His Gly Val Arg Gly Lys Tyr Ala Leu Ala Asp Ala 
            20                  25                  30          


Ser Leu Lys Met Ala Asp Pro Asn Arg Phe Arg Gly Lys Asp Leu Pro 
        35                  40                  45              


Val Glu Asn Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
    50                  55                  60                  


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp 
65                  70                  75                  80  


Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
                85                  90                  95      


Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser 
            100                 105                 110         


Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
        115                 120                 125             


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr 
    130                 135                 140                 


Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser 
145                 150                 155                 160 


Asp Met Pro Met Ala Asp Pro Asn Arg Phe Arg Gly Lys Asn Leu Val 
                165                 170                 175     


Phe His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            180                 185                 190         


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile 
        195                 200                 205             


Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    210                 215                 220                 


Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala 
225                 230                 235                 240 


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn 
                245                 250                 255     


Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            260                 265                 270         


Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr 
        275                 280                 285             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ser Gly Gly Gly Ser 
    290                 295                 300                 


Gly Ser Gly Gly Ser Leu Asp Gln Leu Thr Asp Pro Pro Gly Val Arg 
305                 310                 315                 320 


Arg Val Tyr His Ile Gln Ala Gly Leu Pro Asp Pro Phe Gln Pro Pro 
                325                 330                 335     


Ser Leu Pro Ile Thr Val Tyr Tyr Ala Val Leu Glu Arg Ala Cys Arg 
            340                 345                 350         


Ser Val Leu Leu Asn Ala Pro Ser Glu Ala Pro Gln Ile Val Arg Gly 
        355                 360                 365             


Ala Ser Glu Asp Val Arg Lys Gln Pro Tyr Asn Leu Thr Ile Ala Trp 
    370                 375                 380                 


Phe Arg Met Gly Gly Asn Cys Ala Ile Pro Ile Thr Val Met Glu Tyr 
385                 390                 395                 400 


Thr Glu Cys Ser Tyr Asn Lys Ser Leu Gly Ala Cys Pro Ile Arg Thr 
                405                 410                 415     


Gln Pro Arg Trp Asn Tyr Tyr Asp Ser Phe Ser Ala Val Ser Glu Asp 
            420                 425                 430         


Asn Leu Gly Phe Leu Met His Ala Pro Ala Phe Glu Thr Ala Gly Thr 
        435                 440                 445             


Tyr Leu Arg Leu Val Lys Ile Asn Asp Trp Thr Glu Ile Thr Gln Phe 
    450                 455                 460                 


Ile Leu Glu His Arg Ala Lys Gly Ser Cys Lys Tyr Ala Leu Pro Leu 
465                 470                 475                 480 


Arg Ile Pro Pro Ser Ala Cys Leu Ser Pro Gln Ala Tyr Gln Gln Gly 
                485                 490                 495     


Val Thr Val Asp Ser Ile Gly Met Leu Pro Arg Phe Gly Ser Lys Asn 
            500                 505                 510         


Tyr His Leu Glu Asn Glu Val Ala Arg Leu Lys Lys Leu Val Gly Ser 
        515                 520                 525             


Tyr Ser Leu Lys Ile Ala Gly Trp His Gly Pro Lys Ala Pro Tyr Thr 
    530                 535                 540                 


Ser Thr Leu Leu Pro Pro Glu Leu Ser Glu Thr Pro Asn Ala Thr Gln 
545                 550                 555                 560 


Pro Glu Leu Ala Pro Glu Asp Pro Glu Asp Ser Ala Leu Leu Glu Asp 
                565                 570                 575     


Pro Val Gly Thr Val Ala Pro Gln Ile Pro Pro Asn Trp His Ile Pro 
            580                 585                 590         


Ser Ile Gln Asp Ala Ala Thr Pro Tyr His Pro Pro Ala Thr Pro Asn 
        595                 600                 605             


Asn Met Gly Leu Ile Ala Gly Ala Val Gly Gly Ser Leu Leu Ala Ala 
    610                 615                 620                 


Leu Val Ile Cys Gly Ile Val Tyr Trp Met Arg Arg Arg Thr Gln Lys 
625                 630                 635                 640 


Ala Pro Lys Arg Ile Arg Leu Pro His Ile Arg Glu Asp Asp Gln Pro 
                645                 650                 655     


Ser Ser His Gln Pro Leu Phe Tyr 
            660                 


<210>  10
<211>  2013
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence of chimeric gD-GCN4, scFv HER2 of R-99-2

<400>  10
atgggggggg ctgccgccag gttgggggcc gtgattttgt ttgtcgtcat agtgggcctc       60

catggggtcc gcggcaaata tgccttggcg gatgcctctc tcaagatggc cgaccccaat      120

cgctttcgcg gcaaagacct tccggtcgag aattccgata tccagatgac ccagtccccg      180

agctccctgt ccgcctctgt gggcgatagg gtcaccatca cctgccgtgc cagtcaggat      240

gtgaatactg ctgtagcctg gtatcaacag aaaccaggaa aagctccgaa gcttctgatt      300

tactcggcat ccttcctcta ctctggagtc ccttctcgct tctctggtag ccgttccggg      360

acggatttca ctctgaccat cagcagtctg cagccggaag acttcgcaac ttattactgt      420

cagcaacatt atactactcc tcccacgttc ggacagggta ccaaggtgga gatcaaatcg      480

gatatgccga tggctgatcc gaaccgtttc cgcggtaaga acctggtttt tcattctgag      540

gttcagctgg tggagtctgg cggtggcctg gtgcagccag ggggctcact ccgtttgtcc      600

tgtgcagctt ctggcttcaa cattaaagac acctatatac actgggtgcg tcaggccccg      660

ggtaagggcc tggaatgggt tgcaaggatt tatcctacga atggttatac tagatatgcc      720

gatagcgtca agggccgttt cactataagc gcagacacat ccaaaaacac agcctaccta      780

caaatgaaca gcttaagagc tgaggacact gccgtctatt attgtagccg ctggggaggg      840

gacggcttct atgctatgga ctactggggt caaggaacac tagtcaccgt ctcctcgagt      900

ggcggtggct ctggttccgg tggatccctg gaccagctga ccgaccctcc gggggtccgg      960

cgcgtgtacc acatccaggc gggcctacca gacccgttcc agccccccag cctcccgatc     1020

acggtttact acgccgtgtt ggagcgcgcc tgccgcagcg tgctcctaaa cgcaccgtcg     1080

gaggcccccc agattgtccg cggggcctcc gaagacgtcc ggaaacaacc ctacaacctg     1140

accatcgctt ggtttcggat gggaggcaac tgtgctatcc ccatcacggt catggagtac     1200

accgaatgct cctacaacaa gtctctgggg gcctgtccca tccgaacgca gccccgctgg     1260

aactactatg acagcttcag cgccgtcagc gaggataacc tggggttcct gatgcacgcc     1320

cccgcgtttg agaccgccgg cacgtacctg cggctcgtga agataaacga ctggacggag     1380

attacacagt ttatcctgga gcaccgagcc aagggctcct gtaagtacgc cctcccgctg     1440

cgcatccccc cgtcagcctg cctgtccccc caggcctacc agcagggggt gacggtggac     1500

agcatcggga tgctgccccg cttcatcccc gagaaccagc gcggatccaa gaactaccac     1560

ctggagaacg aggtggccag actgaagaag ctggtgggca gctacagctt gaagatcgcc     1620

gggtggcacg ggcccaaggc cccatacacg agcaccctgc tgcccccgga gctgtccgag     1680

acccccaacg ccacgcagcc agaactcgcc ccggaagacc ccgaggattc ggccctcttg     1740

gaggaccccg tggggacggt ggcgccgcaa atcccaccaa actggcacat accgtcgatc     1800

caggacgccg cgacgcctta ccatcccccg gccaccccga acaacatggg cctgatcgcc     1860

ggcgcggtgg gcggcagtct cctggcagcc ctggtcattt gcggaattgt gtactggatg     1920

cgccgccgca ctcaaaaagc cccaaagcgc atacgcctcc cccacatccg ggaagacgac     1980

cagccgtcct cgcaccagcc cttgttttac tag                                  2013


<210>  11
<211>  670
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the precursor of gD having inserted the 
       scFv to HER2 receptor and the GCN4 peptide as encoded by the 
       construct R-99-2

<400>  11

Met Gly Gly Ala Ala Ala Arg Leu Gly Ala Val Ile Leu Phe Val Val 
1               5                   10                  15      


Ile Val Gly Leu His Gly Val Arg Gly Lys Tyr Ala Leu Ala Asp Ala 
            20                  25                  30          


Ser Leu Lys Met Ala Asp Pro Asn Arg Phe Arg Gly Lys Asp Leu Pro 
        35                  40                  45              


Val Glu Asn Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
    50                  55                  60                  


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp 
65                  70                  75                  80  


Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
                85                  90                  95      


Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser 
            100                 105                 110         


Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
        115                 120                 125             


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr 
    130                 135                 140                 


Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser 
145                 150                 155                 160 


Asp Met Pro Met Ala Asp Pro Asn Arg Phe Arg Gly Lys Asn Leu Val 
                165                 170                 175     


Phe His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            180                 185                 190         


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile 
        195                 200                 205             


Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    210                 215                 220                 


Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala 
225                 230                 235                 240 


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn 
                245                 250                 255     


Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            260                 265                 270         


Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr 
        275                 280                 285             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ser Gly Gly Gly Ser 
    290                 295                 300                 


Gly Ser Gly Gly Ser Leu Asp Gln Leu Thr Asp Pro Pro Gly Val Arg 
305                 310                 315                 320 


Arg Val Tyr His Ile Gln Ala Gly Leu Pro Asp Pro Phe Gln Pro Pro 
                325                 330                 335     


Ser Leu Pro Ile Thr Val Tyr Tyr Ala Val Leu Glu Arg Ala Cys Arg 
            340                 345                 350         


Ser Val Leu Leu Asn Ala Pro Ser Glu Ala Pro Gln Ile Val Arg Gly 
        355                 360                 365             


Ala Ser Glu Asp Val Arg Lys Gln Pro Tyr Asn Leu Thr Ile Ala Trp 
    370                 375                 380                 


Phe Arg Met Gly Gly Asn Cys Ala Ile Pro Ile Thr Val Met Glu Tyr 
385                 390                 395                 400 


Thr Glu Cys Ser Tyr Asn Lys Ser Leu Gly Ala Cys Pro Ile Arg Thr 
                405                 410                 415     


Gln Pro Arg Trp Asn Tyr Tyr Asp Ser Phe Ser Ala Val Ser Glu Asp 
            420                 425                 430         


Asn Leu Gly Phe Leu Met His Ala Pro Ala Phe Glu Thr Ala Gly Thr 
        435                 440                 445             


Tyr Leu Arg Leu Val Lys Ile Asn Asp Trp Thr Glu Ile Thr Gln Phe 
    450                 455                 460                 


Ile Leu Glu His Arg Ala Lys Gly Ser Cys Lys Tyr Ala Leu Pro Leu 
465                 470                 475                 480 


Arg Ile Pro Pro Ser Ala Cys Leu Ser Pro Gln Ala Tyr Gln Gln Gly 
                485                 490                 495     


Val Thr Val Asp Ser Ile Gly Met Leu Pro Arg Phe Ile Pro Glu Asn 
            500                 505                 510         


Gln Arg Gly Ser Lys Asn Tyr His Leu Glu Asn Glu Val Ala Arg Leu 
        515                 520                 525             


Lys Lys Leu Val Gly Ser Tyr Ser Leu Lys Ile Ala Gly Trp His Gly 
    530                 535                 540                 


Pro Lys Ala Pro Tyr Thr Ser Thr Leu Leu Pro Pro Glu Leu Ser Glu 
545                 550                 555                 560 


Thr Pro Asn Ala Thr Gln Pro Glu Leu Ala Pro Glu Asp Pro Glu Asp 
                565                 570                 575     


Ser Ala Leu Leu Glu Asp Pro Val Gly Thr Val Ala Pro Gln Ile Pro 
            580                 585                 590         


Pro Asn Trp His Ile Pro Ser Ile Gln Asp Ala Ala Thr Pro Tyr His 
        595                 600                 605             


Pro Pro Ala Thr Pro Asn Asn Met Gly Leu Ile Ala Gly Ala Val Gly 
    610                 615                 620                 


Gly Ser Leu Leu Ala Ala Leu Val Ile Cys Gly Ile Val Tyr Trp Met 
625                 630                 635                 640 


Arg Arg Arg Thr Gln Lys Ala Pro Lys Arg Ile Arg Leu Pro His Ile 
                645                 650                 655     


Arg Glu Asp Asp Gln Pro Ser Ser His Gln Pro Leu Phe Tyr 
            660                 665                 670 


<210>  12
<211>  20
<212>  PRT
<213>  Artificial sequence

<220>
<223>  GCN4 peptide

<400>  12

Gly Ser Lys Asn Tyr His Leu Glu Asn Glu Val Ala Arg Leu Lys Lys 
1               5                   10                  15      


Leu Val Gly Ser 
            20  


<210>  13
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  GCN4 epitope

<400>  13

Tyr His Leu Glu Asn Glu Val Ala Arg Leu Lys Lys 
1               5                   10          


<210>  14
<211>  281
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  14

Met Ser Glu Tyr Gln Pro Ser Leu Phe Ala Leu Asn Pro Met Gly Phe 
1               5                   10                  15      


Ser Pro Leu Asp Gly Ser Lys Ser Thr Asn Glu Asn Val Ser Ala Ser 
            20                  25                  30          


Thr Ser Thr Ala Lys Pro Met Val Gly Gln Leu Ile Phe Asp Lys Phe 
        35                  40                  45              


Ile Lys Thr Glu Glu Asp Pro Ile Ile Lys Gln Asp Thr Pro Ser Asn 
    50                  55                  60                  


Leu Asp Phe Asp Phe Ala Leu Pro Gln Thr Ala Thr Ala Pro Asp Ala 
65                  70                  75                  80  


Lys Thr Val Leu Pro Ile Pro Glu Leu Asp Asp Ala Val Val Glu Ser 
                85                  90                  95      


Phe Phe Ser Ser Ser Thr Asp Ser Thr Pro Met Phe Glu Tyr Glu Asn 
            100                 105                 110         


Leu Glu Asp Asn Ser Lys Glu Trp Thr Ser Leu Phe Asp Asn Asp Ile 
        115                 120                 125             


Pro Val Thr Thr Asp Asp Val Ser Leu Ala Asp Lys Ala Ile Glu Ser 
    130                 135                 140                 


Thr Glu Glu Val Ser Leu Val Pro Ser Asn Leu Glu Val Ser Thr Thr 
145                 150                 155                 160 


Ser Phe Leu Pro Thr Pro Val Leu Glu Asp Ala Lys Leu Thr Gln Thr 
                165                 170                 175     


Arg Lys Val Lys Lys Pro Asn Ser Val Val Lys Lys Ser His His Val 
            180                 185                 190         


Gly Lys Asp Asp Glu Ser Arg Leu Asp His Leu Gly Val Val Ala Tyr 
        195                 200                 205             


Asn Arg Lys Gln Arg Ser Ile Pro Leu Ser Pro Ile Val Pro Glu Ser 
    210                 215                 220                 


Ser Asp Pro Ala Ala Leu Lys Arg Ala Arg Asn Thr Glu Ala Ala Arg 
225                 230                 235                 240 


Arg Ser Arg Ala Arg Lys Leu Gln Arg Met Lys Gln Leu Glu Asp Lys 
                245                 250                 255     


Val Glu Glu Leu Leu Ser Lys Asn Tyr His Leu Glu Asn Glu Val Ala 
            260                 265                 270         


Arg Leu Lys Lys Leu Val Gly Glu Arg 
        275                 280     


<210>  15
<211>  846
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  15
atgtccgaat atcagccaag tttatttgct ttaaatccaa tgggtttctc accattggat       60

ggttctaaat caaccaacga aaatgtatct gcttccactt ctactgccaa accaatggtt      120

ggccaattga tttttgataa attcatcaag actgaagagg atccaattat caaacaggat      180

accccttcga accttgattt tgattttgct cttccacaaa cggcaactgc acctgatgcc      240

aagaccgttt tgccaattcc ggagctagat gccgctgtag tggaatcttt cttttcgtca      300

agcactgatt caactccaat gtttgagtat gaaaacctag aagacaactc taaagaatgg      360

acatccttgt ttgacaatga cattccagtt accactgacg atgtttcatt ggctgataag      420

gcaattgaat ccactgaaga agtttctctg gtaccatcca atctggaagt ctcgacaact      480

tcattcttac ccactcctgt tctagaagat gctaaactga ctcaaacaag aaaggttaag      540

aaaccaaatt cagtcgttaa gaagtcacat catgttggaa aggatgacga atcgagactg      600

gatcatctag gtgttgttgc ttacaaccgc aaacagcgtt cgattccact ttctccaatt      660

gtgcccgaat ccagtgatcc tgctgctcta aaacgtgcta gaaacactga agccgccagg      720

cgttctcgtg cgagaaagtt gcaaagaatg aaacaacttg aagacaaggt tgaagaattg      780

ctttcgaaaa attatcactt ggaaaatgag gttgccagat taaagaaatt agttggcgaa      840

cgctga                                                                 846


<210>  16
<211>  260
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of scFv HER2 cassette

<400>  16

Glu Asn Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
1               5                   10                  15      


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val 
            20                  25                  30          


Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys 
        35                  40                  45              


Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg 
    50                  55                  60                  


Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
65                  70                  75                  80  


Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr 
                85                  90                  95      


Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser Asp 
            100                 105                 110         


Met Pro Met Ala Asp Pro Asn Arg Phe Arg Gly Lys Asn Leu Val Phe 
        115                 120                 125             


His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro 
    130                 135                 140                 


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys 
145                 150                 155                 160 


Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
                165                 170                 175     


Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp 
            180                 185                 190         


Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr 
        195                 200                 205             


Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
    210                 215                 220                 


Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp 
225                 230                 235                 240 


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ser Gly Gly Gly Ser Gly 
                245                 250                 255     


Ser Gly Gly Ser 
            260 


<210>  17
<211>  275
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of scFv to GCN4 peptide

<400>  17

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly Ala 
            20                  25                  30          


Asp Ala Val Val Thr Gln Glu Ser Ala Leu Thr Thr Ser Pro Gly Glu 
        35                  40                  45              


Thr Val Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser 
    50                  55                  60                  


Asn Tyr Ala Ser Trp Val Gln Glu Lys Pro Asp His Leu Phe Thr Gly 
65                  70                  75                  80  


Leu Ile Gly Gly Thr Asn Asn Arg Ala Pro Gly Val Pro Ala Arg Phe 
                85                  90                  95      


Ser Gly Ser Leu Ile Gly Asp Lys Ala Ala Leu Thr Ile Thr Gly Ala 
            100                 105                 110         


Gln Thr Glu Asp Glu Ala Ile Tyr Phe Cys Ala Leu Trp Tyr Ser Asn 
        115                 120                 125             


His Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Asp Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Ala Pro 
                165                 170                 175     


Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr 
            180                 185                 190         


Asp Tyr Gly Val Asn Trp Val Arg Gln Ser Pro Gly Lys Gly Leu Glu 
        195                 200                 205             


Trp Leu Gly Val Ile Trp Gly Asp Gly Ile Thr Asp Tyr Asn Ser Ala 
    210                 215                 220                 


Leu Lys Ser Arg Leu Ser Val Thr Lys Asp Asn Ser Lys Ser Gln Val 
225                 230                 235                 240 


Phe Leu Lys Met Asn Ser Leu Gln Ser Gly Asp Ser Ala Arg Tyr Tyr 
                245                 250                 255     


Cys Val Thr Gly Leu Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr 
            260                 265                 270         


Val Ser Ser 
        275 


<210>  18
<211>  654
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the scFv to GCN4 peptide comprising human 
       nectin-1 residues

<400>  18

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly Ala 
            20                  25                  30          


Asp Ala Val Val Thr Gln Glu Ser Ala Leu Thr Thr Ser Pro Gly Glu 
        35                  40                  45              


Thr Val Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser 
    50                  55                  60                  


Asn Tyr Ala Ser Trp Val Gln Glu Lys Pro Asp His Leu Phe Thr Gly 
65                  70                  75                  80  


Leu Ile Gly Gly Thr Asn Asn Arg Ala Pro Gly Val Pro Ala Arg Phe 
                85                  90                  95      


Ser Gly Ser Leu Ile Gly Asp Lys Ala Ala Leu Thr Ile Thr Gly Ala 
            100                 105                 110         


Gln Thr Glu Asp Glu Ala Ile Tyr Phe Cys Ala Leu Trp Tyr Ser Asn 
        115                 120                 125             


His Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Asp Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Ala Pro 
                165                 170                 175     


Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr 
            180                 185                 190         


Asp Tyr Gly Val Asn Trp Val Arg Gln Ser Pro Gly Lys Gly Leu Glu 
        195                 200                 205             


Trp Leu Gly Val Ile Trp Gly Asp Gly Ile Thr Asp Tyr Asn Ser Ala 
    210                 215                 220                 


Leu Lys Ser Arg Leu Ser Val Thr Lys Asp Asn Ser Lys Ser Gln Val 
225                 230                 235                 240 


Phe Leu Lys Met Asn Ser Leu Gln Ser Gly Asp Ser Ala Arg Tyr Tyr 
                245                 250                 255     


Cys Val Thr Gly Leu Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr 
            260                 265                 270         


Val Ser Ser Gly Ser Gly Ala Met Ala Lys Pro Thr Asn Trp Ile Glu 
        275                 280                 285             


Gly Thr Gln Ala Val Leu Arg Ala Lys Lys Gly Gln Asp Asp Lys Val 
    290                 295                 300                 


Leu Val Ala Thr Cys Thr Ser Ala Asn Gly Lys Pro Pro Ser Val Val 
305                 310                 315                 320 


Ser Trp Glu Thr Arg Leu Lys Gly Glu Ala Glu Tyr Gln Glu Ile Arg 
                325                 330                 335     


Asn Pro Asn Gly Thr Val Thr Val Ile Ser Arg Tyr Arg Leu Val Pro 
            340                 345                 350         


Ser Arg Glu Ala His Gln Gln Ser Leu Ala Cys Ile Val Asn Tyr His 
        355                 360                 365             


Met Asp Arg Phe Lys Glu Ser Leu Thr Leu Asn Val Gln Tyr Glu Pro 
    370                 375                 380                 


Glu Val Thr Ile Glu Gly Phe Asp Gly Asn Trp Tyr Leu Gln Arg Met 
385                 390                 395                 400 


Asp Val Lys Leu Thr Cys Lys Ala Asp Ala Asn Pro Pro Ala Thr Glu 
                405                 410                 415     


Tyr His Trp Thr Thr Leu Asn Gly Ser Leu Pro Lys Gly Val Glu Ala 
            420                 425                 430         


Gln Asn Arg Thr Leu Phe Phe Lys Gly Pro Ile Asn Tyr Ser Leu Ala 
        435                 440                 445             


Gly Thr Tyr Ile Cys Glu Ala Thr Asn Pro Ile Gly Thr Arg Ser Gly 
    450                 455                 460                 


Gln Val Glu Val Asn Ile Thr Glu Phe Pro Tyr Thr Pro Ser Pro Pro 
465                 470                 475                 480 


Glu His Gly Arg Arg Ala Gly Pro Val Pro Thr Ala Ile Ile Gly Gly 
                485                 490                 495     


Val Ala Gly Ser Ile Leu Leu Val Leu Ile Val Val Gly Gly Ile Val 
            500                 505                 510         


Val Ala Leu Arg Arg Arg Arg His Thr Phe Lys Gly Asp Tyr Ser Thr 
        515                 520                 525             


Lys Lys His Val Tyr Gly Asn Gly Tyr Ser Lys Ala Gly Ile Pro Gln 
    530                 535                 540                 


His His Pro Pro Met Ala Gln Asn Leu Gln Tyr Pro Asp Asp Ser Asp 
545                 550                 555                 560 


Asp Glu Lys Lys Ala Gly Pro Leu Gly Gly Ser Ser Tyr Glu Glu Glu 
                565                 570                 575     


Glu Glu Glu Glu Glu Gly Gly Gly Gly Gly Glu Arg Lys Val Gly Gly 
            580                 585                 590         


Pro His Pro Lys Tyr Asp Glu Asp Ala Lys Arg Pro Tyr Phe Thr Val 
        595                 600                 605             


Asp Glu Ala Glu Ala Arg Gln Asp Gly Tyr Gly Asp Arg Thr Leu Gly 
    610                 615                 620                 


Tyr Gln Tyr Asp Pro Glu Gln Leu Asp Leu Ala Glu Asn Met Val Ser 
625                 630                 635                 640 


Gln Asn Asp Gly Ser Phe Ile Ser Lys Lys Glu Trp Tyr Val 
                645                 650                 


<210>  19
<211>  1965
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence encoding the amino acid sequence of the scFv 
       to GCN4 peptide comprising human nectin-1 residues

<400>  19
atggaaaccg acacccttct tttgtgggtg cttcttcttt gggtgcccgg gagcaccggg       60

gactacccct acgacgtgcc cgactacgcc ggggctgatg ccgtggtgac ccaggagagc      120

gccttgacca caagccccgg ggagaccgtg accttgacct gtagaagcag cacaggggcc      180

gttacaacct ctaactacgc cagctgggtt caggagaagc ccgaccacct tttcaccgga      240

cttatcggag ggaccaacaa cagagccccc ggggtgcctg ctagattcag cgggagcctt      300

attggggaca aggccgccct taccattacc ggggctcaga ccgaagacga ggctatctac      360

ttctgtgctc tttggtacag caaccattgg gtgttcggag gcgggacaaa gcttacagtg      420

cttggaggcg gtggaggcag cggcggaggt gggtctggtg gagggggctc tgggggaggc      480

ggtagcgacg tgcagcttca gcagagcggg cccgggcttg tggccccctc tcagtctctt      540

agcataacgt gcaccgtgag cgggttcagc cttaccgact atggggttaa ctgggtgaga      600

cagtctcctg ggaaggggct tgagtggttg ggagttatct ggggagacgg aatcaccgac      660

tacaacagcg ccttgaagag cagactttct gtgacaaagg acaactctaa gagccaggtg      720

ttccttaaga tgaacagcct tcagagcggg gactctgcca gatactactg cgtgacaggg      780

cttttcgact actggggaca agggaccacc ttgaccgtga gcagcggaag cggagccatg      840

gccaagccca ccaactggat cgaggggaca caggccgtgc ttagagccaa gaaggggcag      900

gacgacaagg ttcttgttgc tacttgcacc agcgccaacg gaaagccccc cagcgtggtg      960

agctgggaga caagattgaa aggggaggcc gagtatcagg agatcagaaa ccctaacggg     1020

accgtgaccg tgatcagcag atacagactt gtgcctagca gagaggccca ccagcagagc     1080

cttgcctgca tcgttaacta ccacatggac agattcaagg agagccttac acttaacgtg     1140

cagtacgaac ccgaggtgac catcgagggg ttcgacggga actggtacct tcagagaatg     1200

gacgtgaagc ttacctgcaa ggccgacgcc aaccctcccg ccaccgagta ccactggacc     1260

acccttaacg ggagccttcc caaaggggtg gaggcccaga acagaaccct tttcttcaag     1320

gggcccatca attacagcct tgccgggacc tacatctgcg aggccaccaa ccccatcggg     1380

accagaagcg gtcaagtgga ggtgaacatc accgagttcc cctacacccc cagcccaccc     1440

gagcacggga gaagagctgg gcccgttccc accgccatca tcggaggggt ggccgggagc     1500

atcttgcttg tgcttatcgt ggtgggtggg attgtggtgg cccttagaag aagaagacat     1560

accttcaaag gggactacag caccaagaag cacgtgtacg ggaacgggta cagcaaggcc     1620

ggaatccctc agcaccatcc acctatggcc cagaaccttc agtaccccga cgacagcgac     1680

gatgagaaga aggctgggcc ccttggtggg agcagctacg aagaggagga agaagaggaa     1740

gagggtggcg gcggtggaga gagaaaagtg ggagggcctc atcccaaata cgacgaggac     1800

gccaagagac cctacttcac cgtggacgag gccgaggcca gacaggacgg gtacggggac     1860

agaacccttg ggtaccagta cgaccccgag cagttggact tggccgagaa catggtgagc     1920

cagaacgacg gaagcttcat ctctaagaag gagtggtacg tgtga                     1965


<210>  20
<211>  74
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD24_galK_f

<400>  20
ctctcaagat ggccgacccc aatcgctttc gcggcaaaga ccttccggtc cctgttgaca       60

attaatcatc ggca                                                         74


<210>  21
<211>  70
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD25_galK_r

<400>  21
tggatgtggt acacgcgccg gacccccgga gggtcggtca gctggtccag tcagcactgt       60

cctgctcctt                                                              70


<210>  22
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer galK_827_f

<400>  22
gcgtgatgtc accattgaag                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer galK_1142_r

<400>  23
tattgttcag cgacagcttg                                                   20


<210>  24
<211>  60
<212>  DNA
<213>  Artificial sequence

<220>
<223>  GCN4 peptide cassette

<400>  24
ggatccaaga actaccacct ggagaacgag gtggccagac tgaagaagct ggtgggcagc       60


<210>  25
<211>  110
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD24_GCN4_fB

<400>  25
ctctcaagat ggccgacccc aatcgctttc gcggcaaaga ccttccggtc ggatccaaga       60

actaccacct ggagaacgag gtggccagac tgaagaagct ggtgggcagc                 110


<210>  26
<211>  110
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD25_GCN4_rB

<400>  26
tggatgtggt acacgcgccg gacccccgga gggtcggtca gctggtccag gctgcccacc       60

agcttcttca gtctggccac ctcgttctcc aggtggtagt tcttggatcc                 110


<210>  27
<211>  1245
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence of chimeric gD-GCN4 of R-81

<400>  27
atgggggggg ctgccgccag gttgggggcc gtgattttgt ttgtcgtcat agtgggcctc       60

catggggtcc gcggcaaata tgccttggcg gatgcctctc tcaagatggc cgaccccaat      120

cgctttcgcg gcaaagacct tccggtcgga tccaagaact accacctgga gaacgaggtg      180

gccagactga agaagctggt gggcagcctg gaccagctga ccgaccctcc gggggtccgg      240

cgcgtgtacc acatccaggc gggcctacca gacccgttcc agccccccag cctcccgatc      300

acggtttact acgccgtgtt ggagcgcgcc tgccgcagcg tgctcctaaa cgcaccgtcg      360

gaggcccccc agattgtccg cggggcctcc gaagacgtcc ggaaacaacc ctacaacctg      420

accatcgctt ggtttcggat gggaggcaac tgtgctatcc ccatcacggt catggagtac      480

accgaatgct cctacaacaa gtctctgggg gcctgtccca tccgaacgca gccccgctgg      540

aactactatg acagcttcag cgccgtcagc gaggataacc tggggttcct gatgcacgcc      600

cccgcgtttg agaccgccgg cacgtacctg cggctcgtga agataaacga ctggacggag      660

attacacagt ttatcctgga gcaccgagcc aagggctcct gtaagtacgc cctcccgctg      720

cgcatccccc cgtcagcctg cctgtccccc caggcctacc agcagggggt gacggtggac      780

agcatcggga tgctgccccg cttcatcccc gagaaccagc gcaccgtcgc cgtatacagc      840

ttgaagatcg ccgggtggca cgggcccaag gccccataca cgagcaccct gctgcccccg      900

gagctgtccg agacccccaa cgccacgcag ccagaactcg ccccggaaga ccccgaggat      960

tcggccctct tggaggaccc cgtggggacg gtggcgccgc aaatcccacc aaactggcac     1020

ataccgtcga tccaggacgc cgcgacgcct taccatcccc cggccacccc gaacaacatg     1080

ggcctgatcg ccggcgcggt gggcggcagt ctcctggcag ccctggtcat ttgcggaatt     1140

gtgtactgga tgcgccgccg cactcaaaaa gccccaaagc gcatacgcct cccccacatc     1200

cgggaagacg accagccgtc ctcgcaccag cccttgtttt actag                     1245


<210>  28
<211>  414
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the precursor of gD having inserted the 
       GCN4 peptide, as encoded by the construct R-81

<400>  28

Met Gly Gly Ala Ala Ala Arg Leu Gly Ala Val Ile Leu Phe Val Val 
1               5                   10                  15      


Ile Val Gly Leu His Gly Val Arg Gly Lys Tyr Ala Leu Ala Asp Ala 
            20                  25                  30          


Ser Leu Lys Met Ala Asp Pro Asn Arg Phe Arg Gly Lys Asp Leu Pro 
        35                  40                  45              


Val Gly Ser Lys Asn Tyr His Leu Glu Asn Glu Val Ala Arg Leu Lys 
    50                  55                  60                  


Lys Leu Val Gly Ser Leu Asp Gln Leu Thr Asp Pro Pro Gly Val Arg 
65                  70                  75                  80  


Arg Val Tyr His Ile Gln Ala Gly Leu Pro Asp Pro Phe Gln Pro Pro 
                85                  90                  95      


Ser Leu Pro Ile Thr Val Tyr Tyr Ala Val Leu Glu Arg Ala Cys Arg 
            100                 105                 110         


Ser Val Leu Leu Asn Ala Pro Ser Glu Ala Pro Gln Ile Val Arg Gly 
        115                 120                 125             


Ala Ser Glu Asp Val Arg Lys Gln Pro Tyr Asn Leu Thr Ile Ala Trp 
    130                 135                 140                 


Phe Arg Met Gly Gly Asn Cys Ala Ile Pro Ile Thr Val Met Glu Tyr 
145                 150                 155                 160 


Thr Glu Cys Ser Tyr Asn Lys Ser Leu Gly Ala Cys Pro Ile Arg Thr 
                165                 170                 175     


Gln Pro Arg Trp Asn Tyr Tyr Asp Ser Phe Ser Ala Val Ser Glu Asp 
            180                 185                 190         


Asn Leu Gly Phe Leu Met His Ala Pro Ala Phe Glu Thr Ala Gly Thr 
        195                 200                 205             


Tyr Leu Arg Leu Val Lys Ile Asn Asp Trp Thr Glu Ile Thr Gln Phe 
    210                 215                 220                 


Ile Leu Glu His Arg Ala Lys Gly Ser Cys Lys Tyr Ala Leu Pro Leu 
225                 230                 235                 240 


Arg Ile Pro Pro Ser Ala Cys Leu Ser Pro Gln Ala Tyr Gln Gln Gly 
                245                 250                 255     


Val Thr Val Asp Ser Ile Gly Met Leu Pro Arg Phe Ile Pro Glu Asn 
            260                 265                 270         


Gln Arg Thr Val Ala Val Tyr Ser Leu Lys Ile Ala Gly Trp His Gly 
        275                 280                 285             


Pro Lys Ala Pro Tyr Thr Ser Thr Leu Leu Pro Pro Glu Leu Ser Glu 
    290                 295                 300                 


Thr Pro Asn Ala Thr Gln Pro Glu Leu Ala Pro Glu Asp Pro Glu Asp 
305                 310                 315                 320 


Ser Ala Leu Leu Glu Asp Pro Val Gly Thr Val Ala Pro Gln Ile Pro 
                325                 330                 335     


Pro Asn Trp His Ile Pro Ser Ile Gln Asp Ala Ala Thr Pro Tyr His 
            340                 345                 350         


Pro Pro Ala Thr Pro Asn Asn Met Gly Leu Ile Ala Gly Ala Val Gly 
        355                 360                 365             


Gly Ser Leu Leu Ala Ala Leu Val Ile Cys Gly Ile Val Tyr Trp Met 
    370                 375                 380                 


Arg Arg Arg Thr Gln Lys Ala Pro Lys Arg Ile Arg Leu Pro His Ile 
385                 390                 395                 400 


Arg Glu Asp Asp Gln Pro Ser Ser His Gln Pro Leu Phe Tyr 
                405                 410                 


<210>  29
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD_ext_f

<400>  29
tccataccga ccacaccgac gaatccc                                           27


<210>  30
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD_ext_r

<400>  30
gagtttgata ccagactgac cgtg                                              24


<210>  31
<211>  74
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer galK_gD35_F

<400>  31
tgaagaagct ggtgggcagc ctggaccagc tgaccgaccc tccgggggtc cctgttgaca       60

attaatcatc ggca                                                         74


<210>  32
<211>  70
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer galK_gD39_R

<400>  32
gtgatcggga ggctgggggg ctggaacggg tctggtaggc ccgcctggat tcagcactgt       60

cctgctcctt                                                              70


<210>  33
<211>  780
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence of scFv HER2 cassette

<400>  33
gagaattccg atatccagat gacccagtcc ccgagctccc tgtccgcctc tgtgggcgat       60

agggtcacca tcacctgccg tgccagtcag gatgtgaata ctgctgtagc ctggtatcaa      120

cagaaaccag gaaaagctcc gaagcttctg atttactcgg catccttcct ctactctgga      180

gtcccttctc gcttctctgg tagccgttcc gggacggatt tcactctgac catcagcagt      240

ctgcagccgg aagacttcgc aacttattac tgtcagcaac attatactac tcctcccacg      300

ttcggacagg gtaccaaggt ggagatcaaa tcggatatgc cgatggctga tccgaaccgt      360

ttccgcggta agaacctggt ttttcattct gaggttcagc tggtggagtc tggcggtggc      420

ctggtgcagc cagggggctc actccgtttg tcctgtgcag cttctggctt caacattaaa      480

gacacctata tacactgggt gcgtcaggcc ccgggtaagg gcctggaatg ggttgcaagg      540

atttatccta cgaatggtta tactagatat gccgatagcg tcaagggccg tttcactata      600

agcgcagaca catccaaaaa cacagcctac ctacaaatga acagcttaag agctgaggac      660

actgccgtct attattgtag ccgctgggga ggggacggct tctatgctat ggactactgg      720

ggtcaaggaa cactagtcac cgtctcctcg agtggcggtg gctctggttc cggtggatcc      780


<210>  34
<211>  70
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD-34-scFvHER2-F

<400>  34
tgaagaagct ggtgggcagc ctggaccagc tgaccgaccc tccgggggtc gagaattccg       60

atatccagat                                                              70


<210>  35
<211>  70
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD-40-scFvHER2-R

<400>  35
gtgatcggga ggctgggggg ctggaacggg tctggtaggc ccgcctggat ggatccaccg       60

gaaccagagc                                                              70


<210>  36
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer scFv_456_r

<400>  36
agctgcacag gacaaacgga gtgagccccc                                        30


<210>  37
<211>  74
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer galK_gD214_F

<400>  37
cctaccagca gggggtgacg gtggacagca tcgggatgct gccccgcttc cctgttgaca       60

attaatcatc ggca                                                         74


<210>  38
<211>  70
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer galK_gD223_R

<400>  38
ctcgtgtatg gggccttggg cccgtgccac ccggcgatct tcaagctgta tcagcactgt       60

cctgctcctt                                                              70


<210>  39
<211>  70
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD213-scFvHER2f

<400>  39
cctaccagca gggggtgacg gtggacagca tcgggatgct gccccgcttc gagaattccg       60

atatccagat                                                              70


<210>  40
<211>  70
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD224-scFvHER2r

<400>  40
ctcgtgtatg gggccttggg cccgtgccac ccggcgatct tcaagctgta ggatccaccg       60

gaaccagagc                                                              70


<210>  41
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gDintforw

<400>  41
ccctacaacc tgaccatcgc ttgg                                              24


<210>  42
<211>  71
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD24-scFvHer2-F

<400>  42
ctctcaagat ggccgacccc aatcgctttc gcggcaaaga ccttccggtc gagaattccg       60

atatccagat g                                                            71


<210>  43
<211>  70
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD25-scFvHer2-R

<400>  43
tggatgtggt acacgcgccg gacccccgga gggtcggtca gctggtccag ggatccaccg       60

gaaccagagc                                                              70


<210>  44
<211>  110
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD213-GCN4-F

<400>  44
cctaccagca gggggtgacg gtggacagca tcgggatgct gccccgcttc ggatccaaga       60

actaccacct ggagaacgag gtggccagac tgaagaagct ggtgggcagc                 110


<210>  45
<211>  110
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD224-GCN4-R

<400>  45
ctcgtgtatg gggccttggg cccgtgccac ccggcgatct tcaagctgta gctgcccacc       60

agcttcttca gtctggccac ctcgttctcc aggtggtagt tcttggatcc                 110


<210>  46
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer HSV_139688_r

<400>  46
ccgacttatc gactgtccac ctttccc                                           27


<210>  47
<211>  74
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD35-galK-F

<400>  47
gctctggttc cggtggatcc ctggaccagc tgaccgaccc tccgggggtc cctgttgaca       60

attaatcatc ggca                                                         74


<210>  48
<211>  70
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD39-galK-R

<400>  48
gtgatcggga ggctgggggg ctggaacggg tctggtaggc ccgcctggat tcagcactgt       60

cctgctcctt                                                              70


<210>  49
<211>  110
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD35-GCN4-F

<400>  49
gctctggttc cggtggatcc ctggaccagc tgaccgaccc tccgggggtc ggatccaaga       60

actaccacct ggagaacgag gtggccagac tgaagaagct ggtgggcagc                 110


<210>  50
<211>  110
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD39-GCN4-R

<400>  50
gtgatcggga ggctgggggg ctggaacggg tctggtaggc ccgcctggat gctgcccacc       60

agcttcttca gtctggccac ctcgttctcc aggtggtagt tcttggatcc                 110


<210>  51
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer scFv4D5 651_f

<400>  51
ggacactgcc gtctattatt gtagccgct                                         29


<210>  52
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gDintrev

<400>  52
ccagtcgttt atcttcacga gccg                                              24


<210>  53
<211>  120
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer gD219-GCN4-F

<400>  53
cctaccagca gggggtgacg gtggacagca tcgggatgct gccccgcttc atccccgaga       60

accagcgcgg atccaagaac taccacctgg agaacgaggt ggccagactg aagaagctgg      120


