                         SEQUENCE LISTING

<110>  ModernaTX, Inc.
 
<120>  mRNA Encoding for VEGF and Methods of Use Thereof

<130>  200369-WO-PCT

<150>  US 62/346,979
<151>  2016-06-07

<150>  US 62/411,091
<151>  2016-10-21

<150>  US 62/432,005
<151>  2016-12-09

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  845
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Modified RNA encoding VEGF-A


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5' 7MeGppp, wherein Me = methyl and p = inorganic phosphate

<220>
<221>  misc_feature
<222>  (1)..(845)
<223>  A = AMP, C = CMP, G = GMP, U = N1-methyl-pseudoUMP

<220>
<221>  modified_base
<222>  (2)..(2)
<223>  G2'OMe, wherein Me is methyl

<220>
<221>  modified_base
<222>  (845)..(845)
<223>  GOH 3'

<400>  1
ggggaaauaa gagagaaaag aagaguaaga agaaauauaa gagccaccau gaacuuucug       60

cugucuuggg ugcauuggag ccuugccuug cugcucuacc uccaccaugc caaguggucc      120

caggcugcac ccauggcaga aggaggaggg cagaaucauc acgaaguggu gaaguucaug      180

gaugucuauc agcgcagcua cugccaucca aucgagaccc ugguggacau cuuccaggag      240

uacccugaug agaucgagua caucuucaag ccauccugug ugccccugau gcgaugcggg      300

ggcugcugca augacgaggg ccuggagugu gugcccacug aggaguccaa caucaccaug      360

cagauuaugc ggaucaaacc ucaccaaggc cagcacauag gagagaugag cuuccuacag      420

cacaacaaau gugaaugcag accaaagaaa gauagagcaa gacaagaaaa ucccuguggg      480

ccuugcucag agcggagaaa gcauuuguuu guacaagauc cgcagacgug uaaauguucc      540

ugcaaaaaca cagacucgcg uugcaaggcg aggcagcuug aguuaaacga acguacuugc      600

agaugugaca agccgaggcg gugauaauag gcuggagccu cgguggccau gcuucuugcc      660

ccuugggccu ccccccagcc ccuccucccc uuccugcacc cguacccccg uggucuuuga      720

auaaagucug agugggcggc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

ucuag                                                                  845


<210>  2
<211>  191
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Asn Phe Leu Leu Ser Trp Val His Trp Ser Leu Ala Leu Leu Leu 
1               5                   10                  15      


Tyr Leu His His Ala Lys Trp Ser Gln Ala Ala Pro Met Ala Glu Gly 
            20                  25                  30          


Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr Gln 
        35                  40                  45              


Arg Ser Tyr Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln Glu 
    50                  55                  60                  


Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro Leu 
65                  70                  75                  80  


Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val Pro 
                85                  90                  95      


Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Arg Ile Lys Pro His 
            100                 105                 110         


Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys Cys 
        115                 120                 125             


Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Asn Pro Cys Gly 
    130                 135                 140                 


Pro Cys Ser Glu Arg Arg Lys His Leu Phe Val Gln Asp Pro Gln Thr 
145                 150                 155                 160 


Cys Lys Cys Ser Cys Lys Asn Thr Asp Ser Arg Cys Lys Ala Arg Gln 
                165                 170                 175     


Leu Glu Leu Asn Glu Arg Thr Cys Arg Cys Asp Lys Pro Arg Arg 
            180                 185                 190     


<210>  3
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Firefly luciferase 5' UTR


<220>
<221>  misc_feature
<222>  (1)..(47)
<223>  A = AMP, C = CMP, G = GMP, U = N1-methyl-pseudoUMP

<400>  3
gggaaauaag agagaaaaga agaguaagaa gaaauauaag agccacc                     47


<210>  4
<211>  1650
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Firefly luciferase ORF of mRNA construct (excluding the stop 
       codon)


<220>
<221>  misc_feature
<222>  (1)..(1650)
<223>  A = AMP, C = CMP, G = GMP, U = N1-methyl-pseudoUMP

<400>  4
auggaagaug cgaagaacau caagaaggga ccugccccgu uuuacccuuu ggaggacggu       60

acagcaggag aacagcucca caaggcgaug aaacgcuacg cccugguccc cggaacgauu      120

gcguuuaccg augcacauau ugagguagac aucacauacg cagaauacuu cgaaaugucg      180

gugaggcugg cggaagcgau gaagagauau ggucuuaaca cuaaucaccg caucguggug      240

uguucggaga acucauugca guuuuucaug ccgguccuug gagcacuuuu caucgggguc      300

gcagucgcgc cagcgaacga caucuacaau gagcgggaac ucuugaauag caugggaauc      360

ucccagccga cggucguguu ugucuccaaa aaggggcugc agaaaauccu caacgugcag      420

aagaagcucc ccauuauuca aaagaucauc auuauggaua gcaagacaga uuaccaaggg      480

uuccagucga uguauaccuu ugugacaucg cauuugccgc caggguuuaa cgaguaugac      540

uucguccccg agucauuuga cagagauaaa accaucgcgc ugauuaugaa uuccucgggu      600

agcaccgguu ugccaaaggg gguggcguug ccccaccgca cugcuugugu gcgguucucg      660

cacgcuaggg auccuaucuu ugguaaucag aucauucccg acacagcaau ccuguccgug      720

guaccuuuuc aucacgguuu uggcauguuc acgacucucg gcuauuugau uugcgguuuc      780

agggucguac uuauguaucg guucgaggaa gaacuguuuu ugagauccuu gcaagauuac      840

aagauccagu cggcccuccu ugugccaacg cuuuucucau ucuuugcgaa aucgacacuu      900

auugauaagu augaccuuuc caaucugcau gagauugccu cagggggagc gccgcuuagc      960

aaggaagucg gggaggcagu ggccaagcgc uuccaccuuc ccggaauucg gcagggauac     1020

gggcucacgg agacaacauc cgcgauccuu aucacgcccg agggugacga uaagccggga     1080

gccgucggaa aagugguccc cuucuuugaa gccaaggucg uagaccucga cacgggaaaa     1140

acccucggag ugaaccagag gggcgagcuc ugcgugagag ggccgaugau caugucaggu     1200

uacgugaaua acccugaagc gacgaaugcg cugaucgaca aggaugggug guugcauucg     1260

ggagacauug ccuauuggga ugaggaugag cacuucuuua ucguagaucg acuuaagagc     1320

uugaucaaau acaaaggcua ucagguagcg ccugccgagc ucgagucaau ccugcuccag     1380

caccccaaca uuuucgacgc cggaguggcc ggguugcccg augacgacgc gggugagcug     1440

ccagcggccg ugguaguccu cgaacauggg aaaacaauga ccgaaaagga gaucguggac     1500

uacguagcau cacaagugac gacugcgaag aaacugaggg gagggguagu cuuuguggac     1560

gaggucccga aaggcuugac ugggaagcuu gacgcucgca aaauccggga aauccugauu     1620

aaggcaaaga aaggcgggaa aaucgcuguc                                      1650


<210>  5
<211>  119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Firefly luciferase 3' UTR


<220>
<221>  misc_feature
<222>  (1)..(119)
<223>  A = AMP, C = CMP, G = GMP, U = N1-methyl-pseudoUMP

<400>  5
ugauaauagg cuggagccuc gguggccaug cuucuugccc cuugggccuc cccccagccc       60

cuccuccccu uccugcaccc guacccccgu ggucuuugaa uaaagucuga gugggcggc       119


<210>  6
<211>  550
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Firefly luciferase

<400>  6

Met Glu Asp Ala Lys Asn Ile Lys Lys Gly Pro Ala Pro Phe Tyr Pro 
1               5                   10                  15      


Leu Glu Asp Gly Thr Ala Gly Glu Gln Leu His Lys Ala Met Lys Arg 
            20                  25                  30          


Tyr Ala Leu Val Pro Gly Thr Ile Ala Phe Thr Asp Ala His Ile Glu 
        35                  40                  45              


Val Asp Ile Thr Tyr Ala Glu Tyr Phe Glu Met Ser Val Arg Leu Ala 
    50                  55                  60                  


Glu Ala Met Lys Arg Tyr Gly Leu Asn Thr Asn His Arg Ile Val Val 
65                  70                  75                  80  


Cys Ser Glu Asn Ser Leu Gln Phe Phe Met Pro Val Leu Gly Ala Leu 
                85                  90                  95      


Phe Ile Gly Val Ala Val Ala Pro Ala Asn Asp Ile Tyr Asn Glu Arg 
            100                 105                 110         


Glu Leu Leu Asn Ser Met Gly Ile Ser Gln Pro Thr Val Val Phe Val 
        115                 120                 125             


Ser Lys Lys Gly Leu Gln Lys Ile Leu Asn Val Gln Lys Lys Leu Pro 
    130                 135                 140                 


Ile Ile Gln Lys Ile Ile Ile Met Asp Ser Lys Thr Asp Tyr Gln Gly 
145                 150                 155                 160 


Phe Gln Ser Met Tyr Thr Phe Val Thr Ser His Leu Pro Pro Gly Phe 
                165                 170                 175     


Asn Glu Tyr Asp Phe Val Pro Glu Ser Phe Asp Arg Asp Lys Thr Ile 
            180                 185                 190         


Ala Leu Ile Met Asn Ser Ser Gly Ser Thr Gly Leu Pro Lys Gly Val 
        195                 200                 205             


Ala Leu Pro His Arg Thr Ala Cys Val Arg Phe Ser His Ala Arg Asp 
    210                 215                 220                 


Pro Ile Phe Gly Asn Gln Ile Ile Pro Asp Thr Ala Ile Leu Ser Val 
225                 230                 235                 240 


Val Pro Phe His His Gly Phe Gly Met Phe Thr Thr Leu Gly Tyr Leu 
                245                 250                 255     


Ile Cys Gly Phe Arg Val Val Leu Met Tyr Arg Phe Glu Glu Glu Leu 
            260                 265                 270         


Phe Leu Arg Ser Leu Gln Asp Tyr Lys Ile Gln Ser Ala Leu Leu Val 
        275                 280                 285             


Pro Thr Leu Phe Ser Phe Phe Ala Lys Ser Thr Leu Ile Asp Lys Tyr 
    290                 295                 300                 


Asp Leu Ser Asn Leu His Glu Ile Ala Ser Gly Gly Ala Pro Leu Ser 
305                 310                 315                 320 


Lys Glu Val Gly Glu Ala Val Ala Lys Arg Phe His Leu Pro Gly Ile 
                325                 330                 335     


Arg Gln Gly Tyr Gly Leu Thr Glu Thr Thr Ser Ala Ile Leu Ile Thr 
            340                 345                 350         


Pro Glu Gly Asp Asp Lys Pro Gly Ala Val Gly Lys Val Val Pro Phe 
        355                 360                 365             


Phe Glu Ala Lys Val Val Asp Leu Asp Thr Gly Lys Thr Leu Gly Val 
    370                 375                 380                 


Asn Gln Arg Gly Glu Leu Cys Val Arg Gly Pro Met Ile Met Ser Gly 
385                 390                 395                 400 


Tyr Val Asn Asn Pro Glu Ala Thr Asn Ala Leu Ile Asp Lys Asp Gly 
                405                 410                 415     


Trp Leu His Ser Gly Asp Ile Ala Tyr Trp Asp Glu Asp Glu His Phe 
            420                 425                 430         


Phe Ile Val Asp Arg Leu Lys Ser Leu Ile Lys Tyr Lys Gly Tyr Gln 
        435                 440                 445             


Val Ala Pro Ala Glu Leu Glu Ser Ile Leu Leu Gln His Pro Asn Ile 
    450                 455                 460                 


Phe Asp Ala Gly Val Ala Gly Leu Pro Asp Asp Asp Ala Gly Glu Leu 
465                 470                 475                 480 


Pro Ala Ala Val Val Val Leu Glu His Gly Lys Thr Met Thr Glu Lys 
                485                 490                 495     


Glu Ile Val Asp Tyr Val Ala Ser Gln Val Thr Thr Ala Lys Lys Leu 
            500                 505                 510         


Arg Gly Gly Val Val Phe Val Asp Glu Val Pro Lys Gly Leu Thr Gly 
        515                 520                 525             


Lys Leu Asp Ala Arg Lys Ile Arg Glu Ile Leu Ile Lys Ala Lys Lys 
    530                 535                 540                 


Gly Gly Lys Ile Ala Val 
545                 550 


<210>  7
<211>  3274
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LacZ nucleotide sequence (5' UTR, ORF, 3' UTR)


<220>
<221>  misc_feature
<222>  (1)..(3274)
<223>  A = AMP, C = CMP, G = GMP, U = N1-methyl-pseudoUMP

<400>  7
ucaagcuuuu ggacccucgu acagaagcua auacgacuca cuauagggaa auaagagaga       60

aaagaagagu aagaagaaau auaagagcca ccauggccuu ggcugucguc cugcaaagaa      120

gagauuggga aaauccugga guuacgcaac ugaauagacu cgccgcacau ccaccguucg      180

cguccuggcg aaauagcgaa gaagcgcgga ccgacagacc uucgcagcag cugcgcucuc      240

ucaacgggga auggcgguuc gcaugguuuc cggcuccuga ggcagucccg gaaagcuggc      300

ucgagugcga ccucccggaa gccgauacgg ugguggugcc gucaaauugg caaaugcaug      360

gauacgacgc ccccaucuac accaacguca cuuacccuau caccgugaau cccccauucg      420

ucccgacuga gaacccgacu ggaugcuaca gccugaccuu uaacguggac gagucguggc      480

ugcaagaagg gcagacucgc aucauuuucg acggagucaa cuccgcguuc caucuuuggu      540

guaacggacg guggguggga uacgggcagg acuccaggcu gccgagcgaa uucgacuugu      600

cagccuuccu gcgcgccggc gaaaaccgcc uggcugucau gguccuuaga uggucggaug      660

gcucguaccu ggaggaucag gacaugugga ggaugucagg gaucuuccgg gaugucucgc      720

ugcuccacaa gccaacuacc cagaucuccg acuuucaugu ggccacccgc uucaacgaug      780

acuucagcag ggcgguucug gaagccgagg ugcaaaugug cggagaacug agggacuacc      840

uccgcgugac ugucucgcuc uggcagggug aaacccaagu ggcuucaggc acugcaccgu      900

ucggaggaga aaucaucgac gaacggggag gauacgccga ucgcgucacc cugcgccuca      960

auguggaaaa uccgaaacug uggucggcag aaaucccuaa uuuguaccgg gccguggugg     1020

agcugcacac cgccgacgga acucugaucg aggccgaggc augcgaugug ggauuccgcg     1080

agguccgcau cgaaaaugga cugcuucugc uuaauggcaa accgcugcuc auccgcggag     1140

ugaacagaca cgagcaucac ccgcugcacg gucaggucau ggaugaacag acuauggugc     1200

aagacauccu gcugaugaaa caaaacaacu ucaacgccgu ucggugcucc cauuacccua     1260

aucacccguu gugguauacc cuuugcgauc gguacggccu cuacguggug gacgaagcga     1320

acaucgagac ucacggaaug gucccuauga accgccucac ugacgacccg agguggcucc     1380

cggcaauguc ggaacgagug acucggaugg ugcagaggga ccgcaaccau ccgucgguga     1440

uaaucugguc gcuggggaac gaaucuggcc acggagcuaa ccacgaugcg cuguaccgcu     1500

ggauuaaguc cguggaccca agccggcccg uccaguacga aggagguggu gcugauacca     1560

cugcaaccga caucaucugc ccaauguaug cgcgggugga ugaggaccaa ccuuucccgg     1620

cggugccaaa gugguccauc aagaaauggc ucucgcugcc cggagaaacg cgcccgcuga     1680

uccugugcga auaugcgcac gcuaugggaa auucacuggg gggauuugcg aaguacuggc     1740

aggcuuuucg acaguacccg agacuccagg guggcuucgu gugggacugg guugaccaga     1800

gccucaucaa auacgaugaa aacggcaacc caugguccgc guacggcgga gacuuuggag     1860

acaccccuaa cgaucgccag uucugcauga acggccuggu guucgccgac agaacuccgc     1920

auccagcccu uacugaggcu aagcaccaac aacaguucuu ccaguucaga cugucggggc     1980

aaacgaucga agugacuucc gaauaccucu uccggcauuc ggacaacgag uugcugcacu     2040

ggauggucgc ccuggaugga aagccccucg ccuccggaga agugccgcuc gacguggcgc     2100

cgcagggaaa gcaguugauc gaguugccgg aacugccaca gcccgaguca gcaggacagc     2160

ucuggcuuac cgucagaguc gugcagccaa augccaccgc cuggucggag gcaggacaua     2220

uuucagccug gcagcagugg cgccucgccg agaaucugag cgugaccuug ccagcagccu     2280

cacacgccau uccgcaucug accacguccg aaauggacuu uuguaucgaa cuggggaaua     2340

agcgcuggca guucaaucgg caaucaggcu uccuguccca gauguggauu ggugacaaga     2400

agcagcuccu gaccccgcug cgcgaucagu ucacucgcgc cccacuugac aacgacauug     2460

gcgugagcga ggccacgcgu aucgauccaa acgcuugggu ggagcgcugg aaggcggcug     2520

gccacuauca ggcggaggcc gcgcuccugc aguguaccgc ggauacccuc gcagacgccg     2580

uucugauuac cacggcgcau gccuggcaac accagggaaa gacccuguuu aucagccgca     2640

aaacuuaccg gaucgauggc agcggccaaa uggcgaucac uguggacguc gagguggcau     2700

cagacacucc acacccagca cggaucggac ucaauugcca acuggcucaa guggcugaga     2760

gagucaauug gcugggccuc ggcccccaag agaacuaccc ugaucggcuu acugccgcau     2820

gcuuugaccg gugggaucug ccucugucgg auauguacac ccccuacgug uucccauccg     2880

agaacggucu gagaugcggu acuagggagu ugaacuacgg accgcaccaa uggagggggg     2940

acuuucaguu caacaucuca agauacagcc agcagcaauu gauggaaacc ucgcaccggc     3000

aucucuugca ugcagaggaa gggaccuggc ugaacaucga uggauuccac augggaauug     3060

guggggauga cuccuggucc ccuagcgugu ccgcggaacu ucagcugucc gccggccggu     3120

accacuacca gcucgugugg ugucaaaagu gauaauaggc uggagccucg guggccaugc     3180

uucuugcccc uugggccucc ccccagcccc uccuccccuu ccugcacccg uacccccgug     3240

gucuuugaau aaagucugag ugggcggcuc uaga                                 3274


<210>  8
<211>  1019
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LacZ

<400>  8

Met Ala Leu Ala Val Val Leu Gln Arg Arg Asp Trp Glu Asn Pro Gly 
1               5                   10                  15      


Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp 
            20                  25                  30          


Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro Ser Gln Gln Leu Arg 
        35                  40                  45              


Ser Leu Asn Gly Glu Trp Arg Phe Ala Trp Phe Pro Ala Pro Glu Ala 
    50                  55                  60                  


Val Pro Glu Ser Trp Leu Glu Cys Asp Leu Pro Glu Ala Asp Thr Val 
65                  70                  75                  80  


Val Val Pro Ser Asn Trp Gln Met His Gly Tyr Asp Ala Pro Ile Tyr 
                85                  90                  95      


Thr Asn Val Thr Tyr Pro Ile Thr Val Asn Pro Pro Phe Val Pro Thr 
            100                 105                 110         


Glu Asn Pro Thr Gly Cys Tyr Ser Leu Thr Phe Asn Val Asp Glu Ser 
        115                 120                 125             


Trp Leu Gln Glu Gly Gln Thr Arg Ile Ile Phe Asp Gly Val Asn Ser 
    130                 135                 140                 


Ala Phe His Leu Trp Cys Asn Gly Arg Trp Val Gly Tyr Gly Gln Asp 
145                 150                 155                 160 


Ser Arg Leu Pro Ser Glu Phe Asp Leu Ser Ala Phe Leu Arg Ala Gly 
                165                 170                 175     


Glu Asn Arg Leu Ala Val Met Val Leu Arg Trp Ser Asp Gly Ser Tyr 
            180                 185                 190         


Leu Glu Asp Gln Asp Met Trp Arg Met Ser Gly Ile Phe Arg Asp Val 
        195                 200                 205             


Ser Leu Leu His Lys Pro Thr Thr Gln Ile Ser Asp Phe His Val Ala 
    210                 215                 220                 


Thr Arg Phe Asn Asp Asp Phe Ser Arg Ala Val Leu Glu Ala Glu Val 
225                 230                 235                 240 


Gln Met Cys Gly Glu Leu Arg Asp Tyr Leu Arg Val Thr Val Ser Leu 
                245                 250                 255     


Trp Gln Gly Glu Thr Gln Val Ala Ser Gly Thr Ala Pro Phe Gly Gly 
            260                 265                 270         


Glu Ile Ile Asp Glu Arg Gly Gly Tyr Ala Asp Arg Val Thr Leu Arg 
        275                 280                 285             


Leu Asn Val Glu Asn Pro Lys Leu Trp Ser Ala Glu Ile Pro Asn Leu 
    290                 295                 300                 


Tyr Arg Ala Val Val Glu Leu His Thr Ala Asp Gly Thr Leu Ile Glu 
305                 310                 315                 320 


Ala Glu Ala Cys Asp Val Gly Phe Arg Glu Val Arg Ile Glu Asn Gly 
                325                 330                 335     


Leu Leu Leu Leu Asn Gly Lys Pro Leu Leu Ile Arg Gly Val Asn Arg 
            340                 345                 350         


His Glu His His Pro Leu His Gly Gln Val Met Asp Glu Gln Thr Met 
        355                 360                 365             


Val Gln Asp Ile Leu Leu Met Lys Gln Asn Asn Phe Asn Ala Val Arg 
    370                 375                 380                 


Cys Ser His Tyr Pro Asn His Pro Leu Trp Tyr Thr Leu Cys Asp Arg 
385                 390                 395                 400 


Tyr Gly Leu Tyr Val Val Asp Glu Ala Asn Ile Glu Thr His Gly Met 
                405                 410                 415     


Val Pro Met Asn Arg Leu Thr Asp Asp Pro Arg Trp Leu Pro Ala Met 
            420                 425                 430         


Ser Glu Arg Val Thr Arg Met Val Gln Arg Asp Arg Asn His Pro Ser 
        435                 440                 445             


Val Ile Ile Trp Ser Leu Gly Asn Glu Ser Gly His Gly Ala Asn His 
    450                 455                 460                 


Asp Ala Leu Tyr Arg Trp Ile Lys Ser Val Asp Pro Ser Arg Pro Val 
465                 470                 475                 480 


Gln Tyr Glu Gly Gly Gly Ala Asp Thr Thr Ala Thr Asp Ile Ile Cys 
                485                 490                 495     


Pro Met Tyr Ala Arg Val Asp Glu Asp Gln Pro Phe Pro Ala Val Pro 
            500                 505                 510         


Lys Trp Ser Ile Lys Lys Trp Leu Ser Leu Pro Gly Glu Thr Arg Pro 
        515                 520                 525             


Leu Ile Leu Cys Glu Tyr Ala His Ala Met Gly Asn Ser Leu Gly Gly 
    530                 535                 540                 


Phe Ala Lys Tyr Trp Gln Ala Phe Arg Gln Tyr Pro Arg Leu Gln Gly 
545                 550                 555                 560 


Gly Phe Val Trp Asp Trp Val Asp Gln Ser Leu Ile Lys Tyr Asp Glu 
                565                 570                 575     


Asn Gly Asn Pro Trp Ser Ala Tyr Gly Gly Asp Phe Gly Asp Thr Pro 
            580                 585                 590         


Asn Asp Arg Gln Phe Cys Met Asn Gly Leu Val Phe Ala Asp Arg Thr 
        595                 600                 605             


Pro His Pro Ala Leu Thr Glu Ala Lys His Gln Gln Gln Phe Phe Gln 
    610                 615                 620                 


Phe Arg Leu Ser Gly Gln Thr Ile Glu Val Thr Ser Glu Tyr Leu Phe 
625                 630                 635                 640 


Arg His Ser Asp Asn Glu Leu Leu His Trp Met Val Ala Leu Asp Gly 
                645                 650                 655     


Lys Pro Leu Ala Ser Gly Glu Val Pro Leu Asp Val Ala Pro Gln Gly 
            660                 665                 670         


Lys Gln Leu Ile Glu Leu Pro Glu Leu Pro Gln Pro Glu Ser Ala Gly 
        675                 680                 685             


Gln Leu Trp Leu Thr Val Arg Val Val Gln Pro Asn Ala Thr Ala Trp 
    690                 695                 700                 


Ser Glu Ala Gly His Ile Ser Ala Trp Gln Gln Trp Arg Leu Ala Glu 
705                 710                 715                 720 


Asn Leu Ser Val Thr Leu Pro Ala Ala Ser His Ala Ile Pro His Leu 
                725                 730                 735     


Thr Thr Ser Glu Met Asp Phe Cys Ile Glu Leu Gly Asn Lys Arg Trp 
            740                 745                 750         


Gln Phe Asn Arg Gln Ser Gly Phe Leu Ser Gln Met Trp Ile Gly Asp 
        755                 760                 765             


Lys Lys Gln Leu Leu Thr Pro Leu Arg Asp Gln Phe Thr Arg Ala Pro 
    770                 775                 780                 


Leu Asp Asn Asp Ile Gly Val Ser Glu Ala Thr Arg Ile Asp Pro Asn 
785                 790                 795                 800 


Ala Trp Val Glu Arg Trp Lys Ala Ala Gly His Tyr Gln Ala Glu Ala 
                805                 810                 815     


Ala Leu Leu Gln Cys Thr Ala Asp Thr Leu Ala Asp Ala Val Leu Ile 
            820                 825                 830         


Thr Thr Ala His Ala Trp Gln His Gln Gly Lys Thr Leu Phe Ile Ser 
        835                 840                 845             


Arg Lys Thr Tyr Arg Ile Asp Gly Ser Gly Gln Met Ala Ile Thr Val 
    850                 855                 860                 


Asp Val Glu Val Ala Ser Asp Thr Pro His Pro Ala Arg Ile Gly Leu 
865                 870                 875                 880 


Asn Cys Gln Leu Ala Gln Val Ala Glu Arg Val Asn Trp Leu Gly Leu 
                885                 890                 895     


Gly Pro Gln Glu Asn Tyr Pro Asp Arg Leu Thr Ala Ala Cys Phe Asp 
            900                 905                 910         


Arg Trp Asp Leu Pro Leu Ser Asp Met Tyr Thr Pro Tyr Val Phe Pro 
        915                 920                 925             


Ser Glu Asn Gly Leu Arg Cys Gly Thr Arg Glu Leu Asn Tyr Gly Pro 
    930                 935                 940                 


His Gln Trp Arg Gly Asp Phe Gln Phe Asn Ile Ser Arg Tyr Ser Gln 
945                 950                 955                 960 


Gln Gln Leu Met Glu Thr Ser His Arg His Leu Leu His Ala Glu Glu 
                965                 970                 975     


Gly Thr Trp Leu Asn Ile Asp Gly Phe His Met Gly Ile Gly Gly Asp 
            980                 985                 990         


Asp Ser Trp Ser Pro Ser Val Ser  Ala Glu Leu Gln Leu  Ser Ala Gly 
        995                 1000                 1005             


Arg Tyr  His Tyr Gln Leu Val  Trp Cys Gln Lys 
    1010                 1015                 


<210>  9
<211>  3057
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LacZ ORF nucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(3057)
<223>  A = AMP, C = CMP, G = GMP, U = N1-methyl-pseudoUMP

<400>  9
auggccuugg cugucguccu gcaaagaaga gauugggaaa auccuggagu uacgcaacug       60

aauagacucg ccgcacaucc accguucgcg uccuggcgaa auagcgaaga agcgcggacc      120

gacagaccuu cgcagcagcu gcgcucucuc aacggggaau ggcgguucgc augguuuccg      180

gcuccugagg cagucccgga aagcuggcuc gagugcgacc ucccggaagc cgauacggug      240

guggugccgu caaauuggca aaugcaugga uacgacgccc ccaucuacac caacgucacu      300

uacccuauca ccgugaaucc cccauucguc ccgacugaga acccgacugg augcuacagc      360

cugaccuuua acguggacga gucguggcug caagaagggc agacucgcau cauuuucgac      420

ggagucaacu ccgcguucca ucuuuggugu aacggacggu gggugggaua cgggcaggac      480

uccaggcugc cgagcgaauu cgacuuguca gccuuccugc gcgccggcga aaaccgccug      540

gcugucaugg uccuuagaug gucggauggc ucguaccugg aggaucagga cauguggagg      600

augucaggga ucuuccggga ugucucgcug cuccacaagc caacuaccca gaucuccgac      660

uuucaugugg ccacccgcuu caacgaugac uucagcaggg cgguucugga agccgaggug      720

caaaugugcg gagaacugag ggacuaccuc cgcgugacug ucucgcucug gcagggugaa      780

acccaagugg cuucaggcac ugcaccguuc ggaggagaaa ucaucgacga acggggagga      840

uacgccgauc gcgucacccu gcgccucaau guggaaaauc cgaaacugug gucggcagaa      900

aucccuaauu uguaccgggc cgugguggag cugcacaccg ccgacggaac ucugaucgag      960

gccgaggcau gcgauguggg auuccgcgag guccgcaucg aaaauggacu gcuucugcuu     1020

aauggcaaac cgcugcucau ccgcggagug aacagacacg agcaucaccc gcugcacggu     1080

caggucaugg augaacagac uauggugcaa gacauccugc ugaugaaaca aaacaacuuc     1140

aacgccguuc ggugcuccca uuacccuaau cacccguugu gguauacccu uugcgaucgg     1200

uacggccucu acguggugga cgaagcgaac aucgagacuc acggaauggu cccuaugaac     1260

cgccucacug acgacccgag guggcucccg gcaaugucgg aacgagugac ucggauggug     1320

cagagggacc gcaaccaucc gucggugaua aucuggucgc uggggaacga aucuggccac     1380

ggagcuaacc acgaugcgcu guaccgcugg auuaaguccg uggacccaag ccggcccguc     1440

caguacgaag gagguggugc ugauaccacu gcaaccgaca ucaucugccc aauguaugcg     1500

cggguggaug aggaccaacc uuucccggcg gugccaaagu gguccaucaa gaaauggcuc     1560

ucgcugcccg gagaaacgcg cccgcugauc cugugcgaau augcgcacgc uaugggaaau     1620

ucacuggggg gauuugcgaa guacuggcag gcuuuucgac aguacccgag acuccagggu     1680

ggcuucgugu gggacugggu ugaccagagc cucaucaaau acgaugaaaa cggcaaccca     1740

ugguccgcgu acggcggaga cuuuggagac accccuaacg aucgccaguu cugcaugaac     1800

ggccuggugu ucgccgacag aacuccgcau ccagcccuua cugaggcuaa gcaccaacaa     1860

caguucuucc aguucagacu gucggggcaa acgaucgaag ugacuuccga auaccucuuc     1920

cggcauucgg acaacgaguu gcugcacugg auggucgccc uggauggaaa gccccucgcc     1980

uccggagaag ugccgcucga cguggcgccg cagggaaagc aguugaucga guugccggaa     2040

cugccacagc ccgagucagc aggacagcuc uggcuuaccg ucagagucgu gcagccaaau     2100

gccaccgccu ggucggaggc aggacauauu ucagccuggc agcaguggcg ccucgccgag     2160

aaucugagcg ugaccuugcc agcagccuca cacgccauuc cgcaucugac cacguccgaa     2220

auggacuuuu guaucgaacu ggggaauaag cgcuggcagu ucaaucggca aucaggcuuc     2280

cugucccaga uguggauugg ugacaagaag cagcuccuga ccccgcugcg cgaucaguuc     2340

acucgcgccc cacuugacaa cgacauuggc gugagcgagg ccacgcguau cgauccaaac     2400

gcuugggugg agcgcuggaa ggcggcuggc cacuaucagg cggaggccgc gcuccugcag     2460

uguaccgcgg auacccucgc agacgccguu cugauuacca cggcgcaugc cuggcaacac     2520

cagggaaaga cccuguuuau cagccgcaaa acuuaccgga ucgauggcag cggccaaaug     2580

gcgaucacug uggacgucga gguggcauca gacacuccac acccagcacg gaucggacuc     2640

aauugccaac uggcucaagu ggcugagaga gucaauuggc ugggccucgg cccccaagag     2700

aacuacccug aucggcuuac ugccgcaugc uuugaccggu gggaucugcc ucugucggau     2760

auguacaccc ccuacguguu cccauccgag aacggucuga gaugcgguac uagggaguug     2820

aacuacggac cgcaccaaug gaggggggac uuucaguuca acaucucaag auacagccag     2880

cagcaauuga uggaaaccuc gcaccggcau cucuugcaug cagaggaagg gaccuggcug     2940

aacaucgaug gauuccacau gggaauuggu ggggaugacu ccuggucccc uagcgugucc     3000

gcggaacuuc agcuguccgc cggccgguac cacuaccagc ucguguggug ucaaaag        3057


