                         SEQUENCE LISTING

<110>  Juno Therapeutics, Inc.
       Tareen, Semih U.
       Sissons, James
       Frohlich, Mark
 
<120>  METHOD OF IDENTIFYING PEPTIDE EPITOPES, MOLECULES THAT BIND SUCH 
       EPITOPES AND RELATED USES

<130>  735042002140

<140> Not Yet Assigned    
<141> Concurrently Herewith 

<150>  US 62/355,211
<151>  2016-06-27

<160>  63    

<170>  PatentIn version 3.5

<210>  1
<211>  358
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Major histocompatibility complex, class I, E, E*0101

<400>  1

Met Val Asp Gly Thr Leu Leu Leu Leu Leu Ser Glu Ala Leu Ala Leu 
1               5                   10                  15      


Thr Gln Thr Trp Ala Gly Ser His Ser Leu Lys Tyr Phe His Thr Ser 
            20                  25                  30          


Val Ser Arg Pro Gly Arg Gly Glu Pro Arg Phe Ile Ser Val Gly Tyr 
        35                  40                  45              


Val Asp Asp Thr Gln Phe Val Arg Phe Asp Asn Asp Ala Ala Ser Pro 
    50                  55                  60                  


Arg Met Val Pro Arg Ala Pro Trp Met Glu Gln Glu Gly Ser Glu Tyr 
65                  70                  75                  80  


Trp Asp Arg Glu Thr Arg Ser Ala Arg Asp Thr Ala Gln Ile Phe Arg 
                85                  90                  95      


Val Asn Leu Arg Thr Leu Arg Gly Tyr Tyr Asn Gln Ser Glu Ala Gly 
            100                 105                 110         


Ser His Thr Leu Gln Trp Met His Gly Cys Glu Leu Gly Pro Asp Arg 
        115                 120                 125             


Arg Phe Leu Arg Gly Tyr Glu Gln Phe Ala Tyr Asp Gly Lys Asp Tyr 
    130                 135                 140                 


Leu Thr Leu Asn Glu Asp Leu Arg Ser Trp Thr Ala Val Asp Thr Ala 
145                 150                 155                 160 


Ala Gln Ile Ser Glu Gln Lys Ser Asn Asp Ala Ser Glu Ala Glu His 
                165                 170                 175     


Gln Arg Ala Tyr Leu Glu Asp Thr Cys Val Glu Trp Leu His Lys Tyr 
            180                 185                 190         


Leu Glu Lys Gly Lys Glu Thr Leu Leu His Leu Glu Pro Pro Lys Thr 
        195                 200                 205             


His Val Thr His His Pro Ile Ser Asp His Glu Ala Thr Leu Arg Cys 
    210                 215                 220                 


Trp Ala Leu Gly Phe Tyr Pro Ala Glu Ile Thr Leu Thr Trp Gln Gln 
225                 230                 235                 240 


Asp Gly Glu Gly His Thr Gln Asp Thr Glu Leu Val Glu Thr Arg Pro 
                245                 250                 255     


Ala Gly Asp Gly Thr Phe Gln Lys Trp Ala Ala Val Val Val Pro Ser 
            260                 265                 270         


Gly Glu Glu Gln Arg Tyr Thr Cys His Val Gln His Glu Gly Leu Pro 
        275                 280                 285             


Glu Pro Val Thr Leu Arg Trp Lys Pro Ala Ser Gln Pro Thr Ile Pro 
    290                 295                 300                 


Ile Val Gly Ile Ile Ala Gly Leu Val Leu Leu Gly Ser Val Val Ser 
305                 310                 315                 320 


Gly Ala Val Val Ala Ala Val Ile Trp Arg Lys Lys Ser Ser Gly Gly 
                325                 330                 335     


Lys Gly Gly Ser Tyr Ser Lys Ala Glu Trp Ser Asp Ser Ala Gln Gly 
            340                 345                 350         


Ser Glu Ser His Ser Leu 
        355             


<210>  2
<211>  1670
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Major histocompatibility complex, class I, E (CDS for amino acids
       15-1091)

<400>  2
cagaggctgg gatcatggta gatggaaccc tccttttact cctctcggag gccctggccc       60

ttacccagac ctgggcgggc tcccactcct tgaagtattt ccacacttcc gtgtcccggc      120

ccggccgcgg ggagccccgc ttcatctctg tgggctacgt ggacgacacc cagttcgtgc      180

gcttcgacaa cgacgccgcg agtccgagga tggtgccgcg ggcgccgtgg atggagcagg      240

aggggtcaga gtattgggac cgggagacac ggagcgccag ggacaccgca cagattttcc      300

gagtgaacct gcggacgctg cgcggctact acaatcagag cgaggccggg tctcacaccc      360

tgcagtggat gcatggctgc gagctggggc ccgacaggcg cttcctccgc gggtatgaac      420

agttcgccta cgacggcaag gattatctca ccctgaatga ggacctgcgc tcctggaccg      480

cggtggacac ggcggctcag atctccgagc aaaagtcaaa tgatgcctct gaggcggagc      540

accagagagc ctacctggaa gacacatgcg tggagtggct ccacaaatac ctggagaagg      600

ggaaggagac gctgcttcac ctggagcccc caaagacaca cgtgactcac caccccatct      660

ctgaccatga ggccaccctg aggtgctggg ccctgggctt ctaccctgcg gagatcacac      720

tgacctggca gcaggatggg gagggccata cccaggacac ggagctcgtg gagaccaggc      780

ctgcagggga tggaaccttc cagaagtggg cagctgtggt ggtgccttct ggagaggagc      840

agagatacac gtgccatgtg cagcatgagg ggctacccga gcccgtcacc ctgagatgga      900

agccggcttc ccagcccacc atccccatcg tgggcatcat tgctggcctg gttctccttg      960

gatctgtggt ctctggagct gtggttgctg ctgtgatatg gaggaagaag agctcaggtg     1020

gaaaaggagg gagctactct aaggctgagt ggagcgacag tgcccagggg tctgagtctc     1080

acagcttgta aagcctgaga cagctgcctt gtgtgcgact gagatgcaca gctgccttgt     1140

gtgcgactga gatgcaggat ttcctcacgc ctcccctatg tgtcttaggg gactctggct     1200

tctctttttg caagggcctc tgaatctgtc tgtgtccctg ttagcacaat gtgaggaggt     1260

agagaaacag tccacctctg tgtctaccat gacccccttc ctcacactga cctgtgttcc     1320

ttccctgttc tcttttctat taaaaataag aacctgggca gagtgcggca gctcatgcct     1380

gtaatcccag cacttaggga ggccgaggag ggcagatcac gaggtcagga gatcgaaacc     1440

atcctggcta acacggtgaa accccgtctc tactaaaaaa tacaaaaaat tagctgggcg     1500

cagaggcacg ggcctgtagt cccagctact caggaggcgg aggcaggaga atggcgtcaa     1560

cccgggaggc ggaggttgca gtgagccagg attgtgcgac tgcactccag cctgggtgac     1620

agggtgaaac gccatctcaa aaaataaaaa ttaaaaaaaa aaaaaaaaaa                1670


<210>  3
<211>  358
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Major histocompatibility complex, class I, E, E*0103

<400>  3

Met Val Asp Gly Thr Leu Leu Leu Leu Leu Ser Glu Ala Leu Ala Leu 
1               5                   10                  15      


Thr Gln Thr Trp Ala Gly Ser His Ser Leu Lys Tyr Phe His Thr Ser 
            20                  25                  30          


Val Ser Arg Pro Gly Arg Gly Glu Pro Arg Phe Ile Ser Val Gly Tyr 
        35                  40                  45              


Val Asp Asp Thr Gln Phe Val Arg Phe Asp Asn Asp Ala Ala Ser Pro 
    50                  55                  60                  


Arg Met Val Pro Arg Ala Pro Trp Met Glu Gln Glu Gly Ser Glu Tyr 
65                  70                  75                  80  


Trp Asp Arg Glu Thr Arg Ser Ala Arg Asp Thr Ala Gln Ile Phe Arg 
                85                  90                  95      


Val Asn Leu Arg Thr Leu Arg Gly Tyr Tyr Asn Gln Ser Glu Ala Gly 
            100                 105                 110         


Ser His Thr Leu Gln Trp Met His Gly Cys Glu Leu Gly Pro Asp Gly 
        115                 120                 125             


Arg Phe Leu Arg Gly Tyr Glu Gln Phe Ala Tyr Asp Gly Lys Asp Tyr 
    130                 135                 140                 


Leu Thr Leu Asn Glu Asp Leu Arg Ser Trp Thr Ala Val Asp Thr Ala 
145                 150                 155                 160 


Ala Gln Ile Ser Glu Gln Lys Ser Asn Asp Ala Ser Glu Ala Glu His 
                165                 170                 175     


Gln Arg Ala Tyr Leu Glu Asp Thr Cys Val Glu Trp Leu His Lys Tyr 
            180                 185                 190         


Leu Glu Lys Gly Lys Glu Thr Leu Leu His Leu Glu Pro Pro Lys Thr 
        195                 200                 205             


His Val Thr His His Pro Ile Ser Asp His Glu Ala Thr Leu Arg Cys 
    210                 215                 220                 


Trp Ala Leu Gly Phe Tyr Pro Ala Glu Ile Thr Leu Thr Trp Gln Gln 
225                 230                 235                 240 


Asp Gly Glu Gly His Thr Gln Asp Thr Glu Leu Val Glu Thr Arg Pro 
                245                 250                 255     


Ala Gly Asp Gly Thr Phe Gln Lys Trp Ala Ala Val Val Val Pro Ser 
            260                 265                 270         


Gly Glu Glu Gln Arg Tyr Thr Cys His Val Gln His Glu Gly Leu Pro 
        275                 280                 285             


Glu Pro Val Thr Leu Arg Trp Lys Pro Ala Ser Gln Pro Thr Ile Pro 
    290                 295                 300                 


Ile Val Gly Ile Ile Ala Gly Leu Val Leu Leu Gly Ser Val Val Ser 
305                 310                 315                 320 


Gly Ala Val Val Ala Ala Val Ile Trp Arg Lys Lys Ser Ser Gly Gly 
                325                 330                 335     


Lys Gly Gly Ser Tyr Ser Lys Ala Glu Trp Ser Asp Ser Ala Gln Gly 
            340                 345                 350         


Ser Glu Ser His Ser Leu 
        355             


<210>  4
<211>  2679
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Major histocompatibility complex, class I, E, E*0103 (CDS for 
       amino acids 127-1203)

<400>  4
gcagactcag ttctcattcc caatgggtgt cgggtttcta gagaagccaa tcagcgtcgc       60

cacgactccc gactataaag tccccatccg gactcaagaa gttctcagga ctcagaggct      120

gggatcatgg tagatggaac cctcctttta ctcctctcgg aggccctggc ccttacccag      180

acctgggcgg gctcccactc cttgaagtat ttccacactt ccgtgtcccg gcccggccgc      240

ggggagcccc gcttcatctc tgtgggctac gtggacgaca cccagttcgt gcgcttcgac      300

aacgacgccg cgagtccgag gatggtgccg cgggcgccgt ggatggagca ggaggggtca      360

gagtattggg accgggagac acggagcgcc agggacaccg cacagatttt ccgagtgaat      420

ctgcggacgc tgcgcggcta ctacaatcag agcgaggccg ggtctcacac cctgcagtgg      480

atgcatggct gcgagctggg gcccgacggg cgcttcctcc gcgggtatga acagttcgcc      540

tacgacggca aggattatct caccctgaat gaggacctgc gctcctggac cgcggtggac      600

acggcggctc agatctccga gcaaaagtca aatgatgcct ctgaggcgga gcaccagaga      660

gcctacctgg aagacacatg cgtggagtgg ctccacaaat acctggagaa ggggaaggag      720

acgctgcttc acctggagcc cccaaagaca cacgtgactc accaccccat ctctgaccat      780

gaggccaccc tgaggtgctg ggccctgggc ttctaccctg cggagatcac actgacctgg      840

cagcaggatg gggagggcca tacccaggac acggagctcg tggagaccag gcctgcaggg      900

gatggaacct tccagaagtg ggcagctgtg gtggtgcctt ctggagagga gcagagatac      960

acgtgccatg tgcagcatga ggggctaccc gagcccgtca ccctgagatg gaagccggct     1020

tcccagccca ccatccccat cgtgggcatc attgctggcc tggttctcct tggatctgtg     1080

gtctctggag ctgtggttgc tgctgtgata tggaggaaga agagctcagg tggaaaagga     1140

gggagctact ctaaggctga gtggagcgac agtgcccagg ggtctgagtc tcacagcttg     1200

taaagcctga gacagctgcc ttgtgtgcga ctgagatgca cagctgcctt gtgtgcgact     1260

gagatgcagg atttcctcac gcctccccta tgtgtcttag gggactctgg cttctctttt     1320

tgcaagggcc tctgaatctg tctgtgtccc tgttagcaca atgtgaggag gtagagaaac     1380

agtccacctc tgtgtctacc atgaccccct tcctcacact gacctgtgtt ccttccctgt     1440

tctcttttct attaaaaata agaacctggg cagagtgcgg cagctcatgc ctgtaatccc     1500

agcacttagg gaggccgagg agggcagatc acgaggtcag gagatcgaaa ccatcctggc     1560

taacacggtg aaaccccgtc tctactaaaa aatacaaaaa attagctggg cgcagaggca     1620

cgggcctgta gtcccagcta ctcaggaggc ggaggcagga gaatggcgtc aacccgggag     1680

gcggaggttg cagtgagcca ggattgtgcg actgcactcc agcctgggtg acagggtgaa     1740

acgccatctc aaaaaataaa aattgaaaaa taaaaaaaga acctggatct caatttaatt     1800

tttcatattc ttgcaatgaa atggacttga ggaagctaag atcatagcta gaaatacaga     1860

taattccaca gcacatctct agcaaattta gcctattcct attctctagc ctattcctta     1920

ccacctgtaa tcttgaccat ataccttgga gttgaatatt gttttcatac tgctgtggtt     1980

tgaatgttcc ctccaacact catgttgaga cttaatccct aatgtggcaa tactgaaagg     2040

tggggccttt gagatgtgat tggatcgtaa ggctgtgcct tcattcatgg gttaatggat     2100

taatgggtta tcacaggaat gggactggtg gctttataag aagaggaaaa gagaactgag     2160

ctagcatgcc cagcccacag agagcctcca ctagagtgat gctaagtgga aatgtgaggt     2220

gcagctgcca cagagggccc ccaccaggga aatgtctagt gtctagtgga tccaggccac     2280

aggagagagt gccttgtgga gcgctgggag caggacctga ccaccaccag gaccccagaa     2340

ctgtggagtc agtggcagca tgcagcgccc ccttgggaaa gctttaggca ccagcctgca     2400

acccattcga gcagccacgt aggctgcacc cagcaaagcc acaggcacgg ggctacctga     2460

ggccttgggg gcccaatccc tgctccagtg tgtccgtgag gcagcacacg aagtcaaaag     2520

agattattct cttcccacag ataccttttc tctcccatga ccctttaaca gcatctgctt     2580

cattcccctc accttcccag gctgatctga ggtaaacttt gaagtaaaat aaaagctgtg     2640

tttgagcatc atttgtattt caaaaaaaaa aaaaaaaaa                            2679


<210>  5
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  leader sequence (residues 3-11 of A0101, A0301, A3601, and 
       Cw1502) / VL9 peptide

<400>  5

Val Met Ala Pro Arg Thr Leu Leu Leu 
1               5                   


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  leader sequence  (residues 3-11 of A0201, A0211, A2403, and 
       A2501) / VL9 peptide

<400>  6

Val Met Ala Pro Arg Thr Leu Val Leu 
1               5                   


<210>  7
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  leader sequence (residues 3-11 of A0702, B06501, and A0801) / VL9
       peptide

<400>  7

Val Met Ala Pro Arg Thr Val Leu Leu 
1               5                   


<210>  8
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  leader sequence (Residues 3-11 of G) / VL9 peptide

<400>  8

Val Met Ala Pro Arg Thr Leu Phe Leu 
1               5                   


<210>  9
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  leader sequence (residues 3-11 of Cw0401) / VL9 peptide

<400>  9

Val Met Ala Pro Arg Thr Leu Ile Leu 
1               5                   


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-C CRISPR guide RNA 1

<400>  10
agcgacgccg cgagtccgag                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-C CRISPR guide RNA 2

<400>  11
ggtcgcagcc agacatcctc                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-C CRISPR guide RNA 3

<400>  12
gacacagaag tacaagcgcc                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-C CRISPR guide RNA 4

<400>  13
tcaccgctat gatgtgtagg                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-C CRISPR guide RNA 5

<400>  14
ctctcagctg ctccgccgca                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-C CRISPR guide RNA 6

<400>  15
cttcctccta cacatcatag                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-A CRISPR guide RNA 1

<400>  16
cgtcctgccg gtacccgcgg                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-A CRISPR guide RNA 2

<400>  17
taccggcagg acgcctacga                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-A CRISPR guide RNA 3

<400>  18
acagcgacgc cgcgagccag                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-A CRISPR guide RNA 4

<400>  19
ccagtcacag actgaccgag                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-A CRISPR guide RNA 5

<400>  20
tccctcctta ccccatctca                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-A CRISPR guide RNA 6

<400>  21
ccaccccatc tctgaccatg                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-B CRISPR guide RNA 1

<400>  22
cgtcgcagcc gtacatgctc                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-B CRISPR guide RNA 2

<400>  23
cgctgtcgaa cctcacgaac                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-B CRISPR guide RNA 3

<400>  24
ggatggcgag gaccaaactc                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-B CRISPR guide RNA 4

<400>  25
cctggctgtc ctagcagttg                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-B CRISPR guide RNA 5

<400>  26
cgtactggtc atgcccgcgg                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-B CRISPR guide RNA 6

<400>  27
ctccgatgac cacaactgct                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A2-1 siRNA

<400>  28
ggattacatc gccctgaaag                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A2-2 siRNA

<400>  29
gcaggagggt ccggagtatt                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A2-3 siRNA

<400>  30
ggacggggag acacggaaag                                                   20


<210>  31
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A2-4 siRNA

<400>  31
gaaagtgaag gcccactca                                                    19


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABC-1 siRNA

<400>  32
gatacctgga gaacgggaag                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABC-2 siRNA

<400>  33
gctgtggtgg tgccttctgg                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABC-3 siRNA

<400>  34
gctactacaa ccagagcgag                                                   20


<210>  35
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABC-4 siRNA

<400>  35
gtggctccgc agatacctg                                                    19


<210>  36
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HLA-A-specific shRNA

<400>  36
caccugccau gugcaugauu uguguaguca ugcugcacau ggcagguguu uuuu             54


<210>  37
<211>  57
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HLA ABC-specific shRNA

<400>  37
ggagaucaca cugaccuggc auuuguguag ugccagguca gugugaucuc cuuuuuu          57


<210>  38
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 1

<400>  38

Pro Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Pro 
            20                  25                  30  


<210>  39
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 2

<400>  39

Gly Ser Ala Asp Asp Ala Lys Lys Asp Ala Ala Lys Lys Asp Gly Lys 
1               5                   10                  15      


Ser 
    


<210>  40
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer (human IgG4hinge) (aa)

<400>  40

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10          


<210>  41
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spacer (human IgG4hinge) (nt)

<400>  41
gaatctaagt acggaccgcc ctgcccccct tgccct                                 36


<210>  42
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge-CH3 spacer (Homo sapiens)

<400>  42

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg 
1               5                   10                  15      


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
            20                  25                  30          


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
        35                  40                  45              


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
    50                  55                  60                  


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
65                  70                  75                  80  


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
                85                  90                  95      


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
            100                 105                 110         


Leu Ser Leu Ser Leu Gly Lys 
        115                 


<210>  43
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge-CH2-CH3 spacer (Homo sapiens)

<400>  43

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  44
<211>  282
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgD-hinge-Fc (Homo sapiens)

<400>  44

Arg Trp Pro Glu Ser Pro Lys Ala Gln Ala Ser Ser Val Pro Thr Ala 
1               5                   10                  15      


Gln Pro Gln Ala Glu Gly Ser Leu Ala Lys Ala Thr Thr Ala Pro Ala 
            20                  25                  30          


Thr Thr Arg Asn Thr Gly Arg Gly Gly Glu Glu Lys Lys Lys Glu Lys 
        35                  40                  45              


Glu Lys Glu Glu Gln Glu Glu Arg Glu Thr Lys Thr Pro Glu Cys Pro 
    50                  55                  60                  


Ser His Thr Gln Pro Leu Gly Val Tyr Leu Leu Thr Pro Ala Val Gln 
65                  70                  75                  80  


Asp Leu Trp Leu Arg Asp Lys Ala Thr Phe Thr Cys Phe Val Val Gly 
                85                  90                  95      


Ser Asp Leu Lys Asp Ala His Leu Thr Trp Glu Val Ala Gly Lys Val 
            100                 105                 110         


Pro Thr Gly Gly Val Glu Glu Gly Leu Leu Glu Arg His Ser Asn Gly 
        115                 120                 125             


Ser Gln Ser Gln His Ser Arg Leu Thr Leu Pro Arg Ser Leu Trp Asn 
    130                 135                 140                 


Ala Gly Thr Ser Val Thr Cys Thr Leu Asn His Pro Ser Leu Pro Pro 
145                 150                 155                 160 


Gln Arg Leu Met Ala Leu Arg Glu Pro Ala Ala Gln Ala Pro Val Lys 
                165                 170                 175     


Leu Ser Leu Asn Leu Leu Ala Ser Ser Asp Pro Pro Glu Ala Ala Ser 
            180                 185                 190         


Trp Leu Leu Cys Glu Val Ser Gly Phe Ser Pro Pro Asn Ile Leu Leu 
        195                 200                 205             


Met Trp Leu Glu Asp Gln Arg Glu Val Asn Thr Ser Gly Phe Ala Pro 
    210                 215                 220                 


Ala Arg Pro Pro Pro Gln Pro Gly Ser Thr Thr Phe Trp Ala Trp Ser 
225                 230                 235                 240 


Val Leu Arg Val Pro Ala Pro Pro Ser Pro Gln Pro Ala Thr Tyr Thr 
                245                 250                 255     


Cys Val Val Ser His Glu Asp Ser Arg Thr Leu Leu Asn Ala Ser Arg 
            260                 265                 270         


Ser Leu Glu Val Ser Tyr Val Thr Asp His 
        275                 280         


<210>  45
<211>  357
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tEGFR

<400>  45

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly 
            20                  25                  30          


Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe 
        35                  40                  45              


Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala 
    50                  55                  60                  


Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu 
65                  70                  75                  80  


Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile 
                85                  90                  95      


Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu 
            100                 105                 110         


Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala 
        115                 120                 125             


Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu 
    130                 135                 140                 


Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr 
145                 150                 155                 160 


Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys 
                165                 170                 175     


Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly 
            180                 185                 190         


Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu 
        195                 200                 205             


Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys 
    210                 215                 220                 


Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu 
225                 230                 235                 240 


Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met 
                245                 250                 255     


Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala 
            260                 265                 270         


His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val 
        275                 280                 285             


Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His 
    290                 295                 300                 


Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro 
305                 310                 315                 320 


Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala 
                325                 330                 335     


Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly 
            340                 345                 350         


Ile Gly Leu Phe Met 
        355         


<210>  46
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A

<400>  46

Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp 
1               5                   10                  15      


Val Glu Glu Asn Pro Gly Pro Arg 
            20                  


<210>  47
<211>  27
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  CD28 (amino acids amino acids 153-179 of Accession No. P10747)

<400>  47

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  48
<211>  66
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  CD28 (amino acids 114-179 of Accession No. P10747)

<400>  48

Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
1               5                   10                  15      


Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
            20                  25                  30          


Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly 
        35                  40                  45              


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
    50                  55                  60                  


Trp Val 
65      


<210>  49
<211>  41
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  CD28 (amino acids 180-220 of P10747)

<400>  49

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  50
<211>  41
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  CD28 (LL to GG)

<400>  50

Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  51
<211>  42
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  4-1BB (amino acids 214-255 of Q07011.1)

<400>  51

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  52
<211>  112
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  CD3 zeta

<400>  52

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  53
<211>  112
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  CD3 zeta

<400>  53

Arg Val Lys Phe Ser Arg Ser Ala Glu Pro Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  54
<211>  112
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  CD3 zeta

<400>  54

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  55
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  55

Pro Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Pro 
        35  


<210>  56
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A

<400>  56

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  57
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A

<400>  57

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  58
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A

<400>  58

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  59
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E2A

<400>  59

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  60
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F2A

<400>  60

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  61
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tEGFR

<400>  61

Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu 
1               5                   10                  15      


Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile 
            20                  25                  30          


Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe 
        35                  40                  45              


Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr 
    50                  55                  60                  


Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn 
65                  70                  75                  80  


Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg 
                85                  90                  95      


Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile 
            100                 105                 110         


Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val 
        115                 120                 125             


Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp 
    130                 135                 140                 


Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn 
145                 150                 155                 160 


Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu 
                165                 170                 175     


Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser 
            180                 185                 190         


Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu 
        195                 200                 205             


Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln 
    210                 215                 220                 


Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly 
225                 230                 235                 240 


Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro 
                245                 250                 255     


His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr 
            260                 265                 270         


Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His 
        275                 280                 285             


Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro 
    290                 295                 300                 


Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala 
305                 310                 315                 320 


Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met 
                325                 330                 335 


<210>  62
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL9 peptide

<400>  62

Val Met Ala Pro Arg Ala Leu Leu Leu 
1               5                   


<210>  63
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL9 peptide

<400>  63

Ser Gln Ala Pro Leu Pro Cys Val Leu 
1               5                   


