                         SEQUENCE LISTING

<110>  bluebird bio, Inc.
       Jarjour, Jordan
       Mann, Jasdeep
 
<120>  BCL11A HOMING ENDONUCLEASE VARIANTS, COMPOSITIONS, AND METHODS OF
        USE

<130>  BLBD-071/04WO 315698-2598

<150>  US 62/414,273
<151>  2016-10-28

<150>  US 62/375,829
<151>  2016-08-16

<150>  US 62/367,465
<151>  2016-07-27

<150>  US 62/366,530
<151>  2016-07-25

<160>  104   

<170>  PatentIn version 3.5

<210>  1
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi subsp. americana (mitochondrion)

<400>  1

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Met Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Cys Val Met Glu Asn Lys Glu His Leu Lys Ile Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Ile Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  2
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi subsp. americana (mitochondrion)

<400>  2

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  3
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi subsp. americana (mitochondrion)


<220>
<221>  MOD_RES
<222>  (1)..(3)
<223>  Any amino acid or absent

<400>  3

Xaa Xaa Xaa Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  4
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi subsp. americana (mitochondrion)


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  4

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  5
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi subsp. americana (mitochondrion)


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  5

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  6
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  6

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Val Leu Ser Ile Gln Asn Arg 
            20                  25                  30          


Asn Asp Tyr Ala Thr Gly Tyr Arg Ile His Leu Thr Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Asn Asn Ala Gly Asp Asn Leu Val Gln Leu Arg 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  7
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  7

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Val Leu Ser Ile Gln Asn Arg 
            20                  25                  30          


Asn Asp Tyr Ala Thr Gly Tyr Arg Ile His Leu Thr Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Asn Asn Thr Gly Asp Asn Leu Val Gln Leu Arg 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  8
<211>  306
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<400>  8

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Val Leu Arg Ile Gln Asn Ser 
            20                  25                  30          


Asn Asp Tyr Ala Thr Gly Tyr Arg Ile His Leu Thr Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Asn Asn Thr Gly Asp Asn Leu Val Gln Leu Arg 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe Ser 
    290                 295                 300                 


Gly Arg 
305     


<210>  9
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (307)..(308)
<223>  Any amino acid or absent

<400>  9

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Val Leu Ser Ile Gln Asn Lys 
            20                  25                  30          


Asn Asn Tyr Ala Thr Gly Tyr Arg Ile His Leu Thr Phe Gln Ile Ile 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Asn Asn Thr Gly Asp Asn Leu Val Gln Leu Arg 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe Ser 
    290                 295                 300                 


Gly Arg Xaa Xaa 
305             


<210>  10
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (307)..(308)
<223>  Any amino acid or absent

<400>  10

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Val Leu Ser Ile Gln Asn Arg 
            20                  25                  30          


Asn Asp Tyr Ala Thr Gly Tyr Arg Ile Arg Leu Thr Phe Gln Ile Ile 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Asn Asn Thr Gly Asp Asn Leu Val Gln Leu Arg 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe Ser 
    290                 295                 300                 


Gly Arg Xaa Xaa 
305             


<210>  11
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (307)..(308)
<223>  Any amino acid or absent

<400>  11

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Val Leu Gly Ile Gln Asn Arg 
            20                  25                  30          


Asn Asp Tyr Ala Thr Gly Tyr Arg Ile Arg Leu Thr Phe Gln Ile Thr 
        35                  40                  45              


Leu Arg Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Asn Asn Thr Gly Asp Asn Leu Val Gln Leu Arg 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe Ser 
    290                 295                 300                 


Gly Arg Xaa Xaa 
305             


<210>  12
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (307)..(308)
<223>  Any amino acid or absent

<400>  12

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Val Leu Ser Ile His Asn Arg 
            20                  25                  30          


Asn Asp Tyr Ala Thr Gly Tyr Arg Ile His Leu Thr Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Asn Asn Thr Gly Asp Asn His Val Gln Leu Arg 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe Ser 
    290                 295                 300                 


Gly Arg Xaa Xaa 
305             


<210>  13
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (307)..(308)
<223>  Any amino acid or absent

<400>  13

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Arg Leu Ser Ile Gln Asn Arg 
            20                  25                  30          


Asn Asp Tyr Ala Thr Gly Tyr Arg Ile His Leu Arg Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Asn Asn Thr Gly Asp Asn Leu Val Gln Leu Arg 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe Ser 
    290                 295                 300                 


Gly Arg Xaa Xaa 
305             


<210>  14
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (307)..(308)
<223>  Any amino acid or absent

<400>  14

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Tyr Leu Ser Ile Gln Asn Arg 
            20                  25                  30          


Asn Asp Tyr Ala Thr Gly Tyr Arg Ile His Leu Arg Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Arg Ile Glu Asn Thr Gly Asp Asn Leu Val Gln Leu Arg 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe Ser 
    290                 295                 300                 


Gly Arg Xaa Xaa 
305             


<210>  15
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (307)..(308)
<223>  Any amino acid or absent

<400>  15

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Val Leu Ser Ile Gln Asn Arg 
            20                  25                  30          


Ser Asp Tyr Ala Thr Gly Tyr Arg Ile His Leu Arg Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Glu Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Asn Asn Thr Gly Asp Asn Leu Val Gln Leu Arg 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe Ser 
    290                 295                 300                 


Gly Arg Xaa Xaa 
305             


<210>  16
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (307)..(308)
<223>  Any amino acid or absent

<400>  16

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Val Leu Ser Ile Gln Asn Arg 
            20                  25                  30          


Asn Asp Tyr Ala Thr Gly Tyr Arg Ile His Leu Arg Phe Gln Ile Gly 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Ala Asn Val Gly Asp Asn Arg Val Gln Leu Val 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe Ser 
    290                 295                 300                 


Gly Arg Xaa Xaa 
305             


<210>  17
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (307)..(308)
<223>  Any amino acid or absent

<400>  17

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Val Leu Ser Ile Gln Asn Arg 
            20                  25                  30          


Asn Asp Tyr Ala Thr Gly Tyr Arg Ile His Leu Arg Phe Gln Ile Gly 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Gln Asn Met Gly Asp Asn Arg Val Gln Leu Arg 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe Ser 
    290                 295                 300                 


Gly Arg Xaa Xaa 
305             


<210>  18
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (307)..(308)
<223>  Any amino acid or absent

<400>  18

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Val Leu Ser Ile Gln Asn Arg 
            20                  25                  30          


Asn Asp Tyr Ala Thr Gly Tyr Arg Ile His Leu Arg Phe Gln Ile Gly 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Leu Asn Val Gly Asp Asn His Val Gln Leu Arg 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe Ser 
    290                 295                 300                 


Gly Arg Xaa Xaa 
305             


<210>  19
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (307)..(308)
<223>  Any amino acid or absent

<400>  19

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Val Leu Ser Ile Gln Asn Arg 
            20                  25                  30          


Asn Asp Tyr Ala Thr Gly Tyr Arg Ile His Leu Arg Phe Gln Ile Val 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Ser Asn Val Gly Asp Asn His Val Gln Leu Arg 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe Ser 
    290                 295                 300                 


Gly Arg Xaa Xaa 
305             


<210>  20
<211>  875
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized megaTAL amino acid sequence

<400>  20

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
465                 470                 475                 480 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                485                 490                 495     


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala 
            500                 505                 510         


Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His 
        515                 520                 525             


Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val 
    530                 535                 540                 


Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg 
545                 550                 555                 560 


Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly 
                565                 570                 575     


Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe 
            580                 585                 590         


Ala Asp Ala Glu Gly Ser Phe Val Leu Ser Ile Gln Asn Arg Asn Asp 
        595                 600                 605             


Tyr Ala Thr Gly Tyr Arg Ile His Leu Thr Phe Gln Ile Thr Leu His 
    610                 615                 620                 


Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val 
625                 630                 635                 640 


Gly Lys Ile Asn Asn Ala Gly Asp Asn Leu Val Gln Leu Arg Val Tyr 
                645                 650                 655     


Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro 
            660                 665                 670         


Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe 
        675                 680                 685             


Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu 
    690                 695                 700                 


Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu 
705                 710                 715                 720 


Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn 
                725                 730                 735     


Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser Gly Glu 
            740                 745                 750         


Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala Arg Val 
        755                 760                 765             


Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp Lys Asn 
    770                 775                 780                 


Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile Tyr Glu 
785                 790                 795                 800 


Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu Lys Phe 
                805                 810                 815     


Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu 
            820                 825                 830         


Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys 
        835                 840                 845             


Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile 
    850                 855                 860                 


Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg 
865                 870                 875 


<210>  21
<211>  875
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized megaTAL amino acid sequence

<400>  21

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
465                 470                 475                 480 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                485                 490                 495     


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala 
            500                 505                 510         


Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His 
        515                 520                 525             


Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val 
    530                 535                 540                 


Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg 
545                 550                 555                 560 


Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly 
                565                 570                 575     


Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe 
            580                 585                 590         


Ala Asp Ala Glu Gly Ser Phe Val Leu Ser Ile Gln Asn Arg Asn Asp 
        595                 600                 605             


Tyr Ala Thr Gly Tyr Arg Ile His Leu Thr Phe Gln Ile Thr Leu His 
    610                 615                 620                 


Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val 
625                 630                 635                 640 


Gly Lys Ile Asn Asn Thr Gly Asp Asn Leu Val Gln Leu Arg Val Tyr 
                645                 650                 655     


Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro 
            660                 665                 670         


Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe 
        675                 680                 685             


Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu 
    690                 695                 700                 


Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu 
705                 710                 715                 720 


Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn 
                725                 730                 735     


Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp 
            740                 745                 750         


Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala Arg Val 
        755                 760                 765             


Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp Lys Asn 
    770                 775                 780                 


Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile Tyr Glu 
785                 790                 795                 800 


Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu Lys Phe 
                805                 810                 815     


Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu 
            820                 825                 830         


Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys 
        835                 840                 845             


Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile 
    850                 855                 860                 


Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg 
865                 870                 875 


<210>  22
<211>  1116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized megaTAL amino acid sequence

<400>  22

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
465                 470                 475                 480 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                485                 490                 495     


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala 
            500                 505                 510         


Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His 
        515                 520                 525             


Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val 
    530                 535                 540                 


Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg 
545                 550                 555                 560 


Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly 
                565                 570                 575     


Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe 
            580                 585                 590         


Ala Asp Ala Glu Gly Ser Phe Val Leu Ser Ile Gln Asn Arg Asn Asp 
        595                 600                 605             


Tyr Ala Thr Gly Tyr Arg Ile His Leu Thr Phe Gln Ile Thr Leu His 
    610                 615                 620                 


Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val 
625                 630                 635                 640 


Gly Lys Ile Asn Asn Ala Gly Asp Asn Leu Val Gln Leu Arg Val Tyr 
                645                 650                 655     


Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro 
            660                 665                 670         


Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe 
        675                 680                 685             


Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu 
    690                 695                 700                 


Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu 
705                 710                 715                 720 


Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn 
                725                 730                 735     


Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser Gly Glu 
            740                 745                 750         


Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala Arg Val 
        755                 760                 765             


Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp Lys Asn 
    770                 775                 780                 


Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile Tyr Glu 
785                 790                 795                 800 


Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu Lys Phe 
                805                 810                 815     


Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu 
            820                 825                 830         


Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys 
        835                 840                 845             


Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile 
    850                 855                 860                 


Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe Ala Ser Thr 
865                 870                 875                 880 


Gly Ser Glu Pro Pro Arg Ala Glu Thr Phe Val Phe Leu Asp Leu Glu 
                885                 890                 895     


Ala Thr Gly Leu Pro Asn Met Asp Pro Glu Ile Ala Glu Ile Ser Leu 
            900                 905                 910         


Phe Ala Val His Arg Ser Ser Leu Glu Asn Pro Glu Arg Asp Asp Ser 
        915                 920                 925             


Gly Ser Leu Val Leu Pro Arg Val Leu Asp Lys Leu Thr Leu Cys Met 
    930                 935                 940                 


Cys Pro Glu Arg Pro Phe Thr Ala Lys Ala Ser Glu Ile Thr Gly Leu 
945                 950                 955                 960 


Ser Ser Glu Ser Leu Met His Cys Gly Lys Ala Gly Phe Asn Gly Ala 
                965                 970                 975     


Val Val Arg Thr Leu Gln Gly Phe Leu Ser Arg Gln Glu Gly Pro Ile 
            980                 985                 990         


Cys Leu Val Ala His Asn Gly Phe  Asp Tyr Asp Phe Pro  Leu Leu Cys 
        995                 1000                 1005             


Thr Gly  Leu Gln Arg Leu Gly  Ala His Leu Pro Gln  Asp Thr Val 
    1010                 1015                 1020             


Cys Leu  Asp Thr Leu Pro Ala  Leu Arg Gly Leu Asp  Arg Ala His 
    1025                 1030                 1035             


Ser His  Gly Thr Arg Ala Gln  Gly Arg Lys Ser Tyr  Ser Leu Ala 
    1040                 1045                 1050             


Ser Leu  Phe His Arg Tyr Phe  Gln Ala Glu Pro Ser  Ala Ala His 
    1055                 1060                 1065             


Ser Ala  Glu Gly Asp Val His  Thr Leu Leu Leu Ile  Phe Leu His 
    1070                 1075                 1080             


Arg Ala  Pro Glu Leu Leu Ala  Trp Ala Asp Glu Gln  Ala Arg Ser 
    1085                 1090                 1095             


Trp Ala  His Ile Glu Pro Met  Tyr Val Pro Pro Asp  Gly Pro Ser 
    1100                 1105                 1110             


Leu Glu  Ala 
    1115     


<210>  23
<211>  1116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized megaTAL amino acid sequence

<400>  23

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
465                 470                 475                 480 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                485                 490                 495     


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala 
            500                 505                 510         


Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His 
        515                 520                 525             


Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val 
    530                 535                 540                 


Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg 
545                 550                 555                 560 


Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly 
                565                 570                 575     


Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe 
            580                 585                 590         


Ala Asp Ala Glu Gly Ser Phe Val Leu Ser Ile Gln Asn Arg Asn Asp 
        595                 600                 605             


Tyr Ala Thr Gly Tyr Arg Ile His Leu Thr Phe Gln Ile Thr Leu His 
    610                 615                 620                 


Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val 
625                 630                 635                 640 


Gly Lys Ile Asn Asn Thr Gly Asp Asn Leu Val Gln Leu Arg Val Tyr 
                645                 650                 655     


Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro 
            660                 665                 670         


Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe 
        675                 680                 685             


Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu 
    690                 695                 700                 


Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu 
705                 710                 715                 720 


Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn 
                725                 730                 735     


Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp 
            740                 745                 750         


Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala Arg Val 
        755                 760                 765             


Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp Lys Asn 
    770                 775                 780                 


Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile Tyr Glu 
785                 790                 795                 800 


Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu Lys Phe 
                805                 810                 815     


Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu 
            820                 825                 830         


Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys 
        835                 840                 845             


Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile 
    850                 855                 860                 


Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe Ala Ser Thr 
865                 870                 875                 880 


Gly Ser Glu Pro Pro Arg Ala Glu Thr Phe Val Phe Leu Asp Leu Glu 
                885                 890                 895     


Ala Thr Gly Leu Pro Asn Met Asp Pro Glu Ile Ala Glu Ile Ser Leu 
            900                 905                 910         


Phe Ala Val His Arg Ser Ser Leu Glu Asn Pro Glu Arg Asp Asp Ser 
        915                 920                 925             


Gly Ser Leu Val Leu Pro Arg Val Leu Asp Lys Leu Thr Leu Cys Met 
    930                 935                 940                 


Cys Pro Glu Arg Pro Phe Thr Ala Lys Ala Ser Glu Ile Thr Gly Leu 
945                 950                 955                 960 


Ser Ser Glu Ser Leu Met His Cys Gly Lys Ala Gly Phe Asn Gly Ala 
                965                 970                 975     


Val Val Arg Thr Leu Gln Gly Phe Leu Ser Arg Gln Glu Gly Pro Ile 
            980                 985                 990         


Cys Leu Val Ala His Asn Gly Phe  Asp Tyr Asp Phe Pro  Leu Leu Cys 
        995                 1000                 1005             


Thr Gly  Leu Gln Arg Leu Gly  Ala His Leu Pro Gln  Asp Thr Val 
    1010                 1015                 1020             


Cys Leu  Asp Thr Leu Pro Ala  Leu Arg Gly Leu Asp  Arg Ala His 
    1025                 1030                 1035             


Ser His  Gly Thr Arg Ala Gln  Gly Arg Lys Ser Tyr  Ser Leu Ala 
    1040                 1045                 1050             


Ser Leu  Phe His Arg Tyr Phe  Gln Ala Glu Pro Ser  Ala Ala His 
    1055                 1060                 1065             


Ser Ala  Glu Gly Asp Val His  Thr Leu Leu Leu Ile  Phe Leu His 
    1070                 1075                 1080             


Arg Ala  Pro Glu Leu Leu Ala  Trp Ala Asp Glu Gln  Ala Arg Ser 
    1085                 1090                 1095             


Trp Ala  His Ile Glu Pro Met  Tyr Val Pro Pro Asp  Gly Pro Ser 
    1100                 1105                 1110             


Leu Glu  Ala 
    1115     


<210>  24
<211>  29
<212>  DNA
<213>  Homo sapiens

<400>  24
cctggagcct gtgataaaag caactgtta                                         29


<210>  25
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  25
cagttgcttt tatcacaggc tc                                                22


<210>  26
<211>  11
<212>  DNA
<213>  Homo sapiens

<400>  26
agtctagtgc a                                                            11


<210>  27
<211>  39
<212>  DNA
<213>  Homo sapiens

<400>  27
agtctagtgc aagcttacag ttgcttttat cacaggctc                              39


<210>  28
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant target site

<400>  28
cagttgcttt tataaccttt ta                                                22


<210>  29
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant CTD target site

<400>  29
tttccacttt tatcacaggc tc                                                22


<210>  30
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized I-SmaMI target site

<400>  30
tatcctccat tatcaggtgt ac                                                22


<210>  31
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  31
cttccaggaa ttctttggcc tg                                                22


<210>  32
<211>  7078
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant surface display plasmid

<400>  32
gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt       60

cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata      120

atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt      180

aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa      240

atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata      300

gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct      360

tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata      420

aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact      480

attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa      540

atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg      600

ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg      660

ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt      720

attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt      780

gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg      840

ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa      900

cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt      960

gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag     1020

tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt     1080

gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga     1140

ccgaaggagc taaccgcttt ttttcacaac atgggggatc atgtaactcg ccttgatcgt     1200

tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta     1260

gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg     1320

caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc     1380

cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt     1440

atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg     1500

ggcagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg     1560

attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa     1620

cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa     1680

atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga     1740

tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg     1800

ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact     1860

ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac     1920

cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg     1980

gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg     2040

gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga     2100

acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc     2160

gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg     2220

agggagcttc caggggggaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc     2280

tgacttgagc gtcgattttt gtgatgctcg tcaggggggc cgagcctatg gaaaaacgcc     2340

agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt     2400

cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc     2460

gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc     2520

ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac     2580

aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact     2640

cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg     2700

agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcggaatt     2760

aaccctcact aaagggaaca aaagctgggt acccgacagg ttatcagcaa caacacagtc     2820

atatccattc tcaattagct ctaccacagt gtgtgaacca atgtatccag caccacctgt     2880

aaccaaaaca attttagaag tactttcact ttgtaactga gctgtcattt atattgaatt     2940

ttcaaaaatt cttacttttt ttttggatgg acgcaaagaa gtttaataat catattacat     3000

ggcattacca ccatatacat atccatatac atatccatat ctaatcttac ttatatgttg     3060

tggaaatgta aagagcccca ttatcttagc ctaaaaaaac cttctctttg gaactttcag     3120

taatacgctt aactgctcat tgctatattg aagtacggat tagaagccgc cgagcgggtg     3180

acagccctcc gaaggaagac tctcctccgt gcgtcctcgt cttcaccggt cgcgttcctg     3240

aaacgcagat gtgcctcgcg ccgcactgct ccgaacaata aagattctac aatactagct     3300

tttatggtta tgaagaggaa aaattggcag taacctggcc ccacaaacct tcaaatgaac     3360

gaatcaaatt aacaaccata ggatgataat gcgattagtt ttttagcctt atttctgggg     3420

taattaatca gcgaagcgat gatttttgat ctattaacag atatataaat gcaaaaactg     3480

cataaccact ttaactaata ctttcaacat tttcggtttg tattacttct tattcaaatg     3540

taataaaaga tcgaatccta cttcatacat tttcaattaa gatgcagtta cttcgctgtt     3600

tttcaatatt ttctgttatt gcttcagttt tagcacagga actgacaact atatgcgagc     3660

aaatcccctc accaacttta gaatcgacgc cgtactcttt gtcaacgact actattttgg     3720

ccaacgggaa ggcaatgcaa ggagtttttg aatattacaa atcagtaacg tttgtcagta     3780

attgcggttc tcacccctca acaactagca aaggcagccc cataaacaca cagtatgttt     3840

ttaaggacaa tagctcgacg attgaaggta gatacccata cgacgttcca gactacgctc     3900

tgcaggctag tggtggagga ggctctggtg gaggcggtag cggaggcgga gggtcggcta     3960

gctccatcaa cccatggatt ctgactggtt tcactgatgc cgaaggatca ttcatgctaa     4020

gaatccgtaa cacgaacaac cggtcagtag ggtactacac ttcactggta ttcgaaatca     4080

ctctgcacaa caaggacaaa tcgattcttg agaatatcca gtcgacttgg aaggtcggca     4140

caatcaacaa ccgaggcgac ggcaccgcca gactgagcgt cactcgtttc gaagatttga     4200

aagtgattat cgaccacttc gagaaatatc cgctgattac ccagaaattg ggcgattaca     4260

agttgtttaa acaggcattc agcgtcatgg agaacaaaga acatcttaag gagaatggga     4320

ttaaggagct cgtacgaatc aaagctaaga tgaattgggg tctcaatgac gaattgaaaa     4380

aagcatttcc agagaacatc agcaaagagc gcccccttat caataagaac attccgaatc     4440

tcaaatggct ggctggattc acatctggtg aaggcacatt ctacgtgcac ctagcaaagt     4500

ctgaagctag cggcaaggta tacgtgcgac tgaggttcat aatcggccag cacatcagag     4560

acaagaacct gatgaattca ttgataacat acctaggctg tggtacgatc caggagaaga     4620

acaggtctaa gggcagtatg ctccacttca tagtaactaa attcagcgat atcaacgaca     4680

agatcattcc ggtattccag gaaaatactc tgattggcgt caaactcgag gactttgaag     4740

attggtgcaa ggttgccaaa ttgatcgaag agaagaaaca cctgaccgaa tccggtttgg     4800

atgagattaa gaaaatcaag ctgaacatga acaaaggtcg ttctagagaa caaaagttaa     4860

tttctgaaga ggacttgtaa gatctgataa caacagtgta gatgtaacaa aatcgacttt     4920

gttcccactg tacttttagc tcgtacaaaa tacaatatac ttttcatttc tccgtaaaca     4980

acatgttttc ccatgtaata tccttttcta tttttcgttc cgttaccaac tttacacata     5040

ctttatatag ctattcactt ctatacacta aaaaactaag acaattttaa ttttgctgcc     5100

tgccatattt caatttgtta taaattccta taatttatcc tattagtagc taaaaaaaga     5160

tgaatgtgaa tcgaatccta agagaattga gctccaattc gccctatagt gagtcgtatt     5220

acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac     5280

ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca     5340

ccgatcgcct ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg     5400

cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc     5460

cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc     5520

ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct     5580

cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac     5640

ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac     5700

tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat     5760

ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa     5820

aatattaacg tttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt     5880

tcacaccgca ggcaagtgca caaacaatac ttaaataaat actactcagt aataacctat     5940

ttcttagcat ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc     6000

acacctccgc ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt     6060

tctggcgtca gtccaccagc taacataaaa tgtaagcttt cggggctctc ttgccttcca     6120

acccagtcag aaatcgagtt ccaatccaaa agttcacctg tcccacctgc ttctgaatca     6180

aacaagggaa taaacgaatg aggtttctgt gaagctgcac tgagtagtat gttgcagtct     6240

tttggaaata cgagtctttt aataactggc aaaccgagga actcttggta ttcttgccac     6300

gactcatctc catgcagttg gacgatatca atgccgtaat cattgaccag agccaaaaca     6360

tcctccttag gttgattacg aaacacgcca accaagtatt tcggagtgcc tgaactattt     6420

ttatatgctt ttacaagact tgaaattttc cttgcaataa ccgggtcaat tgttctcttt     6480

ctattgggca cacatataat acccagcaag tcagcatcgg aatcaagagc acattctgcg     6540

gcctctgtgc tctgcaagcc gcaaactttc accaatggac cagaactacc tgtgaaatta     6600

ataacagaca tactccaagc tgcctttgtg tgcttaatca cgtatactca cgtgctcaat     6660

agtcaccaat gccctccctc ttggccctct ccttttcttt tttcgaccga attaattctt     6720

aatcggcaaa aaaagaaaag ctccggatca agattgtacg taaggtgaca agctattttt     6780

caataaagaa tatcttccac tactgccatc tggcgtcata actgcaaagt acacatatat     6840

tacgatgctg tctattaaat gcttcctata ttatatatat agtaatgtcg tttatggtgc     6900

actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca     6960

cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg     7020

accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcga       7078


<210>  33
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide sequence for a central 4 array for an 
       I-OnuI LHE variant


<220>
<221>  misc_feature
<222>  (10)..(13)
<223>  n is a, c, g, or t

<400>  33
cttccaggan nnntttggcc tg                                                22


<210>  34
<211>  7243
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide sequence of an I-OnuI LHE variant 
       surface display plasmid

<400>  34
gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt       60

cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata      120

atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt      180

aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa      240

atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata      300

gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct      360

tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata      420

aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact      480

attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa      540

atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg      600

ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg      660

ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt      720

attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt      780

gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg      840

ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa      900

cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt      960

gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag     1020

tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt     1080

gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga     1140

ccgaaggagc taaccgcttt ttttcacaac atgggggatc atgtaactcg ccttgatcgt     1200

tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta     1260

gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg     1320

caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc     1380

cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt     1440

atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg     1500

ggcagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg     1560

attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa     1620

cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa     1680

atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga     1740

tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg     1800

ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact     1860

ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac     1920

cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg     1980

gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg     2040

gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga     2100

acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc     2160

gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg     2220

agggagcttc caggggggaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc     2280

tgacttgagc gtcgattttt gtgatgctcg tcaggggggc cgagcctatg gaaaaacgcc     2340

agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt     2400

cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc     2460

gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc     2520

ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac     2580

aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact     2640

cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg     2700

agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcggaatt     2760

aaccctcact aaagggaaca aaagctgggt acccgacagg ttatcagcaa caacacagtc     2820

atatccattc tcaattagct ctaccacagt gtgtgaacca atgtatccag caccacctgt     2880

aaccaaaaca attttagaag tactttcact ttgtaactga gctgtcattt atattgaatt     2940

ttcaaaaatt cttacttttt ttttggatgg acgcaaagaa gtttaataat catattacat     3000

ggcattacca ccatatacat atccatatac atatccatat ctaatcttac ttatatgttg     3060

tggaaatgta aagagcccca ttatcttagc ctaaaaaaac cttctctttg gaactttcag     3120

taatacgctt aactgctcat tgctatattg aagtacggat tagaagccgc cgagcgggtg     3180

acagccctcc gaaggaagac tctcctccgt gcgtcctcgt cttcaccggt cgcgttcctg     3240

aaacgcagat gtgcctcgcg ccgcactgct ccgaacaata aagattctac aatactagct     3300

tttatggtta tgaagaggaa aaattggcag taacctggcc ccacaaacct tcaaatgaac     3360

gaatcaaatt aacaaccata ggatgataat gcgattagtt ttttagcctt atttctgggg     3420

taattaatca gcgaagcgat gatttttgat ctattaacag atatataaat gcaaaaactg     3480

cataaccact ttaactaata ctttcaacat tttcggtttg tattacttct tattcaaatg     3540

taataaaaga tcgaatccta cttcatacat tttcaattaa gatgcagtta cttcgctgtt     3600

tttcaatatt ttctgttatt gcttcagttt tagcacagga actgacaact atatgcgagc     3660

aaatcccctc accaacttta gaatcgacgc cgtactcttt gtcaacgact actattttgg     3720

ccaacgggaa ggcaatgcaa ggagtttttg aatattacaa atcagtaacg tttgtcagta     3780

attgcggttc tcacccctca acaactagca aaggcagccc cataaacaca cagtatgttt     3840

ttaaggacaa tagctcgacg attgaaggta gatacccata cgacgttcca gactacgctc     3900

tgcaggctag tggtggagga ggctctggtg gaggcggtag cggaggcgga gggtcggcta     3960

gctccatcaa cccatggatt ctgactggtt tcgctgatgc cgaaggaagc ttcgtgctaa     4020

gtatccaaaa cagaaacgat tatgctactg gttacagaat tcacctgaca ttccaaatca     4080

ccctgcacaa caaggacaaa tcgattctgg agaatatcca gtcgacttgg aaggtcggca     4140

aaatcaataa cacgggcgac aacctcgtcc aactgagagt ctaccgtttc gaagatttga     4200

aagtgattat cgaccacttc gagaaatatc cgctgataac acagaaattg ggcgattaca     4260

agttgtttaa acaggcattc agcgtcatgg agaacaaaga acatcttaag gagaatggga     4320

ttaaggagct cgtacgaatc aaagctaaga tgaattgggg tctcaatgac gaattgaaaa     4380

aagcatttcc agagaacatt agcaaagagc gcccccttat caataagaac attccgaatt     4440

tcaaatggct ggctggattc acatctggtg atggctcctt cttcgtgcgc ctaagaaagt     4500

ctaatgttaa tgctagagta cgtgtgcaac tggtattcga gatctcacag cacatcagag     4560

acaagaacct gatgaattca ttgataacat acctaggctg tggtcacatc tacgagggaa     4620

acaaatctga gcgcagttgg ctccaattca gagtagaaaa attcagcgat atcaacgaca     4680

agatcattcc ggtattccag gaaaatactc tgattggcgt caaactcgag gactttgaag     4740

attggtgcaa ggttgccaaa ttgatcgaag agaagaaaca cctgaccgaa tccggtttgg     4800

atgagattaa gaaaatcaag ctgaacatga acaaaggtcg tgtcttctct agaggcggtt     4860

ccagaagcgg atctggtact ggcgaacaga aactcataag cgaagaagac cttagcggga     4920

ctggagagca aaagttgatt tctgaggagg atttgtcggg aaccggggag cagaagttaa     4980

tcagtgaaga ggatctcagt ggaacgggcg aacaaaagtt gatctcggag gaagacttat     5040

aatgagatct gataacaaca gtgtagatgt aacaaaatcg actttgttcc cactgtactt     5100

ttagctcgta caaaatacaa tatacttttc atttctccgt aaacaacatg ttttcccatg     5160

taatatcctt ttctattttt cgttccgtta ccaactttac acatacttta tatagctatt     5220

cacttctata cactaaaaaa ctaagacaat tttaattttg ctgcctgcca tatttcaatt     5280

tgttataaat tcctataatt tatcctatta gtagctaaaa aaagatgaat gtgaatcgaa     5340

tcctaagaga attgagctcc aattcgccct atagtgagtc gtattacaat tcactggccg     5400

tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag     5460

cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcctttccc     5520

aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc     5580

gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc     5640

tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa     5700

tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact     5760

tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt     5820

gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa     5880

ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt     5940

aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac     6000

aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaggcaa     6060

gtgcacaaac aatacttaaa taaatactac tcagtaataa cctatttctt agcatttttg     6120

acgaaatttg ctattttgtt agagtctttt acaccatttg tctccacacc tccgcttaca     6180

tcaacaccaa taacgccatt taatctaagc gcatcaccaa cattttctgg cgtcagtcca     6240

ccagctaaca taaaatgtaa gctttcgggg ctctcttgcc ttccaaccca gtcagaaatc     6300

gagttccaat ccaaaagttc acctgtccca cctgcttctg aatcaaacaa gggaataaac     6360

gaatgaggtt tctgtgaagc tgcactgagt agtatgttgc agtcttttgg aaatacgagt     6420

cttttaataa ctggcaaacc gaggaactct tggtattctt gccacgactc atctccatgc     6480

agttggacga catcaatgcc gtaatcattg accagagcca aaacatcctc cttaggttga     6540

ttacgaaaca cgccaaccaa gtatttcgga gtgcctgaac tatttttata tgcttttaca     6600

agacttgaaa ttttccttgc aataaccggg tcaattgttc tctttctatt gggcacacat     6660

ataataccca gcaagtcagc atcggaatca agagcacatt ctgcggcctc tgtgctctgc     6720

aagccgcaaa ctttcaccaa tggaccagaa ctacctgtga aattaataac agacatactc     6780

caagctgcct ttgtgtgctt aatcacgtat actcacgtgc tcaatagtca ccaatgccct     6840

ccctcttggc cctctccttt tcttttttcg accgaattaa ttcttaatcg gcaaaaaaag     6900

aaaagctccg gatcaagatt gtacgtaagg tgacaagcta tttttcaata aagaatatct     6960

tccactactg ccatctggcg tcataactgc aaagtacaca tatattacga tgctgtctat     7020

taaatgcttc ctatattata tatatagtaa tgtcgtttat ggtgcactct cagtacaatc     7080

tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc     7140

tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc     7200

tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cga                       7243


<210>  35
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide sequence for a central 4 array for an 
       I-OnuI LHE variant


<220>
<221>  misc_feature
<222>  (10)..(13)
<223>  n is a, c, g, or t

<400>  35
cagttgcttn nnncacaggc tc                                                22


<210>  36
<211>  2634
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthesized mRNA encoding a megaTAL

<400>  36
augggauccg cgccaccuaa gaagaaacgc aaagucgugg aucuacgcac gcucggcuac       60

agucagcagc agcaagagaa gaucaaaccg aaggugcguu cgacaguggc gcagcaccac      120

gaggcacugg ugggccaugg guuuacacac gcgcacaucg uugcgcucag ccaacacccg      180

gcagcguuag ggaccgucgc ugucacguau cagcacauaa ucacggcguu gccagaggcg      240

acacacgaag acaucguugg cgucggcaaa cagugguccg gcgcacgcgc ccuggaggcc      300

uugcucacgg augcggggga guugagaggu ccgccguuac aguuggacac aggccaacuu      360

gugaagauug caaaacgugg cggcgugacc gcaauggagg cagugcaugc aucgcgcaau      420

gcacugacgg gugccccccu gaacuuaaca cccgaucaag ugguagcgau agcgucaaau      480

aucgggggua aacaggcuuu ggagacggua cagcgguuau ugccgguacu cugccaggac      540

cacggauuga caccggacca agugguggcg auugcgucca auaacggagg caagcaggca      600

cuagagaccg uccaacggcu ucuucccguu cuuugucagg aucaugggcu aaccccugau      660

cagguagucg cuauagcuuc aaauggcggg ggcaagcaag cacuggagac cguucaacga      720

cuccugccag ugcucugcca agaccacgga cuuacgccag aucagguggu ugcuauugcc      780

ucccacgaug gcgggaaaca agcguuggaa acugugcaga gacuguuacc ugucuugugu      840

caagaccacg gccucacgcc agaucaggug guagccauag cgucgaaugg aggugguaag      900

caagcccuug aaacggucca gcgucuucug ccgguguugu gccaggacca cggacuaacg      960

ccggaucagg ucguagccau ugcuucaaau aucggcggca aacaggcgcu agagacaguc     1020

cagcgccucu ugccuguguu augccaggau cacggcuuaa ccccagacca aguuguggcu     1080

auugcaucua acaauggugg caaacaagcc uuggagacag ugcaacgauu acugccuguc     1140

uuaugucagg aucauggccu gacgcccgau cagguagugg caaucgcauc uaacggcgga     1200

gguaagcaag cacuggagac uguccagaga uuguuacccg uacuauguca agaucauggu     1260

uugacgccug aucagguugu ugcgauagcc agcaacaacg gagggaaaca ggcucuugaa     1320

accguacagc gacuucuccc agucuugugc caagaucacg ggcuuacucc ugaucaaguc     1380

guagcuaucg ccagccacga cggugggaaa caggcccugg aaaccguaca acgucuccuc     1440

ccaguacuuu gucaagacca cggguugacu ccggaucaag ucgucgcgau cgcgagcaau     1500

auagggggga agcaggcgcu ggaaagcauu guggcccagc ugagccggcc ugauccggcg     1560

uuggccgcgu ugaccaacga ccaccucguc gccuuggccu gccucggcgg acguccugcc     1620

auggaugcag ugaaaaaggg auugccgcac gcgccggaau ugaucagaag agucaaucgc     1680

cguauuggcg aacgcacguc ccaucgcguu gcgauaucua gagugggagg aagcucucgc     1740

agagagucca ucaacccaug gauucugacu gguuucgcug augccgaagg aagcuucgug     1800

cuaaguaucc aaaacagaaa cgauuaugcu acugguuaca gaauucaccu gacauuccaa     1860

aucacccugc acaacaagga caaaucgauu cuggagaaua uccagucgac uuggaagguc     1920

ggcaaaauca auaacacggg cgacaaccuc guccaacuga gagucuaccg uuucgaagau     1980

uugaaaguga uuaucgacca cuucgagaaa uauccgcuga uaacacagaa auugggcgau     2040

uacaaguugu uuaaacaggc auucagcguc auggagaaca aagaacaucu uaaggagaau     2100

gggauuaagg agcucguacg aaucaaagcu aagaugaauu ggggucucaa ugacgaauug     2160

aaaaaagcau uuccagagaa cauuagcaaa gagcgccccc uuaucaauaa gaacauuccg     2220

aauuucaaau ggcuggcugg auucacaucu ggugauggcu ccuucuucgu gcgccuaaga     2280

aagucuaaug uuaaugcuag aguacgugug caacugguau ucgagaucuc acagcacauc     2340

agagacaaga accugaugaa uucauugaua acauaccuag gcugugguca caucuacgag     2400

ggaaacaaau cugagcgcag uuggcuccaa uucagaguag aaaaauucag cgauaucaac     2460

gacaagauca uuccgguauu ccaggaaaau acucugauug gcgucaaacu cgaggacuuu     2520

gaagauuggu gcaagguugc caaauugauc gaagagaaga aacaccugac cgaauccggu     2580

uuggaugaga uuaagaaaau caagcugaac augaacaaag gucgugucuu cuaa           2634


<210>  37
<211>  3351
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthesized mRNA encoding a megaTAL

<400>  37
augggauccg cgccaccuaa gaagaaacgc aaagucgugg aucuacgcac gcucggcuac       60

agucagcagc agcaagagaa gaucaaaccg aaggugcguu cgacaguggc gcagcaccac      120

gaggcacugg ugggccaugg guuuacacac gcgcacaucg uugcgcucag ccaacacccg      180

gcagcguuag ggaccgucgc ugucacguau cagcacauaa ucacggcguu gccagaggcg      240

acacacgaag acaucguugg cgucggcaaa cagugguccg gcgcacgcgc ccuggaggcc      300

uugcucacgg augcggggga guugagaggu ccgccguuac aguuggacac aggccaacuu      360

gugaagauug caaaacgugg cggcgugacc gcaauggagg cagugcaugc aucgcgcaau      420

gcacugacgg gugccccccu gaacuuaaca cccgaucaag ugguagcgau agcgucaaau      480

aucgggggua aacaggcuuu ggagacggua cagcgguuau ugccgguacu cugccaggac      540

cacggauuga caccggacca agugguggcg auugcgucca auaacggagg caagcaggca      600

cuagagaccg uccaacggcu ucuucccguu cuuugucagg aucaugggcu aaccccugau      660

cagguagucg cuauagcuuc aaauggcggg ggcaagcaag cacuggagac cguucaacga      720

cuccugccag ugcucugcca agaccacgga cuuacgccag aucagguggu ugcuauugcc      780

ucccacgaug gcgggaaaca agcguuggaa acugugcaga gacuguuacc ugucuugugu      840

caagaccacg gccucacgcc agaucaggug guagccauag cgucgaaugg aggugguaag      900

caagcccuug aaacggucca gcgucuucug ccgguguugu gccaggacca cggacuaacg      960

ccggaucagg ucguagccau ugcuucaaau aucggcggca aacaggcgcu agagacaguc     1020

cagcgccucu ugccuguguu augccaggau cacggcuuaa ccccagacca aguuguggcu     1080

auugcaucua acaauggugg caaacaagcc uuggagacag ugcaacgauu acugccuguc     1140

uuaugucagg aucauggccu gacgcccgau cagguagugg caaucgcauc uaacggcgga     1200

gguaagcaag cacuggagac uguccagaga uuguuacccg uacuauguca agaucauggu     1260

uugacgccug aucagguugu ugcgauagcc agcaacaacg gagggaaaca ggcucuugaa     1320

accguacagc gacuucuccc agucuugugc caagaucacg ggcuuacucc ugaucaaguc     1380

guagcuaucg ccagccacga cggugggaaa caggcccugg aaaccguaca acgucuccuc     1440

ccaguacuuu gucaagacca cggguugacu ccggaucaag ucgucgcgau cgcgagcaau     1500

auagggggga agcaggcgcu ggaaagcauu guggcccagc ugagccggcc ugauccggcg     1560

uuggccgcgu ugaccaacga ccaccucguc gccuuggccu gccucggcgg acguccugcc     1620

auggaugcag ugaaaaaggg auugccgcac gcgccggaau ugaucagaag agucaaucgc     1680

cguauuggcg aacgcacguc ccaucgcguu gcgauaucua gagugggagg aagcucucgc     1740

agagagucca ucaacccaug gauucugacu gguuucgcug augccgaagg aagcuucgug     1800

cuaaguaucc aaaacagaaa cgauuaugcu acugguuaca gaauucaccu gacauuccaa     1860

aucacccugc acaacaagga caaaucgauu cuggagaaua uccagucgac uuggaagguc     1920

ggcaaaauca auaacacggg cgacaaccuc guccaacuga gagucuaccg uuucgaagau     1980

uugaaaguga uuaucgacca cuucgagaaa uauccgcuga uaacacagaa auugggcgau     2040

uacaaguugu uuaaacaggc auucagcguc auggagaaca aagaacaucu uaaggagaau     2100

gggauuaagg agcucguacg aaucaaagcu aagaugaauu ggggucucaa ugacgaauug     2160

aaaaaagcau uuccagagaa cauuagcaaa gagcgccccc uuaucaauaa gaacauuccg     2220

aauuucaaau ggcuggcugg auucacaucu ggugauggcu ccuucuucgu gcgccuaaga     2280

aagucuaaug uuaaugcuag aguacgugug caacugguau ucgagaucuc acagcacauc     2340

agagacaaga accugaugaa uucauugaua acauaccuag gcugugguca caucuacgag     2400

ggaaacaaau cugagcgcag uuggcuccaa uucagaguag aaaaauucag cgauaucaac     2460

gacaagauca uuccgguauu ccaggaaaau acucugauug gcgucaaacu cgaggacuuu     2520

gaagauuggu gcaagguugc caaauugauc gaagagaaga aacaccugac cgaauccggu     2580

uuggaugaga uuaagaaaau caagcugaac augaacaaag gucgugucuu cgcuagcacc     2640

gguucugagc caccucgggc ugagaccuuu guauuccugg accuagaagc cacugggcuc     2700

ccaaacaugg acccugagau ugcagagaua ucccuuuuug cuguucaccg cucuucccug     2760

gagaacccag aacgggauga uucugguucc uuggugcugc cccguguucu ggacaagcuc     2820

acacugugca ugugcccgga gcgccccuuu acugccaagg ccagugagau uacugguuug     2880

agcagcgaaa gccugaugca cugcgggaag gcugguuuca auggcgcugu gguaaggaca     2940

cugcagggcu uccuaagccg ccaggagggc cccaucugcc uuguggccca caauggcuuc     3000

gauuaugacu ucccacugcu gugcacgggg cuacaacguc ugggugccca ucugccccaa     3060

gacacugucu gccuggacac acugccugca uugcggggcc uggaccgugc ucacagccac     3120

ggcaccaggg cucaaggccg caaaagcuac agccuggcca gucucuucca ccgcuacuuc     3180

caggcugaac ccagugcugc ccauucagca gaaggugaug ugcacacccu gcuucugauc     3240

uuccugcauc gugcuccuga gcugcucgcc ugggcagaug agcaggcccg cagcugggcu     3300

cauauugagc ccauguacgu gccaccugau gguccaagcc ucgaagccug a              3351


<210>  38
<211>  711
<212>  RNA
<213>  Mus musculus

<400>  38
augucugagc caccucgggc ugagaccuuu guauuccugg accuagaagc cacugggcuc       60

ccaaacaugg acccugagau ugcagagaua ucccuuuuug cuguucaccg cucuucccug      120

gagaacccag aacgggauga uucugguucc uuggugcugc cccguguucu ggacaagcuc      180

acacugugca ugugcccgga gcgccccuuu acugccaagg ccagugagau uacugguuug      240

agcagcgaaa gccugaugca cugcgggaag gcugguuuca auggcgcugu gguaaggaca      300

cugcagggcu uccuaagccg ccaggagggc cccaucugcc uuguggccca caauggcuuc      360

gauuaugacu ucccacugcu gugcacgggg cuacaacguc ugggugccca ucugccccaa      420

gacacugucu gccuggacac acugccugca uugcggggcc uggaccgugc ucacagccac      480

ggcaccaggg cucaaggccg caaaagcuac agccuggcca gucucuucca ccgcuacuuc      540

caggcugaac ccagugcugc ccauucagca gaaggugaug ugcacacccu gcuucugauc      600

uuccugcauc gugcuccuga gcugcucgcc ugggcagaug agcaggcccg cagcugggcu      660

cauauugagc ccauguacgu gccaccugau gguccaagcc ucgaagccug a               711


<210>  39
<211>  236
<212>  PRT
<213>  Mus musculus

<400>  39

Met Ser Glu Pro Pro Arg Ala Glu Thr Phe Val Phe Leu Asp Leu Glu 
1               5                   10                  15      


Ala Thr Gly Leu Pro Asn Met Asp Pro Glu Ile Ala Glu Ile Ser Leu 
            20                  25                  30          


Phe Ala Val His Arg Ser Ser Leu Glu Asn Pro Glu Arg Asp Asp Ser 
        35                  40                  45              


Gly Ser Leu Val Leu Pro Arg Val Leu Asp Lys Leu Thr Leu Cys Met 
    50                  55                  60                  


Cys Pro Glu Arg Pro Phe Thr Ala Lys Ala Ser Glu Ile Thr Gly Leu 
65                  70                  75                  80  


Ser Ser Glu Ser Leu Met His Cys Gly Lys Ala Gly Phe Asn Gly Ala 
                85                  90                  95      


Val Val Arg Thr Leu Gln Gly Phe Leu Ser Arg Gln Glu Gly Pro Ile 
            100                 105                 110         


Cys Leu Val Ala His Asn Gly Phe Asp Tyr Asp Phe Pro Leu Leu Cys 
        115                 120                 125             


Thr Gly Leu Gln Arg Leu Gly Ala His Leu Pro Gln Asp Thr Val Cys 
    130                 135                 140                 


Leu Asp Thr Leu Pro Ala Leu Arg Gly Leu Asp Arg Ala His Ser His 
145                 150                 155                 160 


Gly Thr Arg Ala Gln Gly Arg Lys Ser Tyr Ser Leu Ala Ser Leu Phe 
                165                 170                 175     


His Arg Tyr Phe Gln Ala Glu Pro Ser Ala Ala His Ser Ala Glu Gly 
            180                 185                 190         


Asp Val His Thr Leu Leu Leu Ile Phe Leu His Arg Ala Pro Glu Leu 
        195                 200                 205             


Leu Ala Trp Ala Asp Glu Gln Ala Arg Ser Trp Ala His Ile Glu Pro 
    210                 215                 220                 


Met Tyr Val Pro Pro Asp Gly Pro Ser Leu Glu Ala 
225                 230                 235     


<210>  40
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  40

Gly Gly Gly 
1           


<210>  41
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  41

Asp Gly Gly Gly Ser 
1               5   


<210>  42
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  42

Thr Gly Glu Lys Pro 
1               5   


<210>  43
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  43

Gly Gly Arg Arg 
1               


<210>  44
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  44

Gly Gly Gly Gly Ser 
1               5   


<210>  45
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  45

Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Val Asp 
1               5                   10                  


<210>  46
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  46

Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 
1               5                   10                  15      


Leu Asp 
        


<210>  47
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  47

Gly Gly Arg Arg Gly Gly Gly Ser 
1               5               


<210>  48
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  48

Leu Arg Gln Arg Asp Gly Glu Arg Pro 
1               5                   


<210>  49
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  49

Leu Arg Gln Lys Asp Gly Gly Gly Ser Glu Arg Pro 
1               5                   10          


<210>  50
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  50

Leu Arg Gln Lys Asp Gly Gly Gly Ser Gly Gly Gly Ser Glu Arg Pro 
1               5                   10                  15      


<210>  51
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa is any amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa = Gly or Ser

<400>  51

Glu Xaa Xaa Tyr Xaa Gln Xaa 
1               5           


<210>  52
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  52

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  53
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  53

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  54
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  54

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  55
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  55

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  56
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  56

Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10                  


<210>  57
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  57

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  58
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  58

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  59
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  59

Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10              


<210>  60
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  60

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro 
            20              


<210>  61
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  61

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  62
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  62

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  63
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  63

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210>  64
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  64

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  65
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  65

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  66
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  66

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  67
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  67

Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  68
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  68

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  69
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  69

Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
1               5                   10                  15      


Pro 
    


<210>  70
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  70

Gln Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  71
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  71

Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
1               5                   10                  15      


Asp Val Glu Ser Asn Pro Gly Pro 
            20                  


<210>  72
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  72

Val Thr Glu Leu Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro 
1               5                   10                  15      


Arg Pro Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys 
            20                  25                  30          


Ile Val Ala Pro Val Lys Gln Thr 
        35                  40  


<210>  73
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  73

Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  74
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  74

Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys Ile Val 
1               5                   10                  15      


Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
            20                  25                  30          


Asp Val Glu Ser Asn Pro Gly Pro 
        35                  40  


<210>  75
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  75

Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Thr Leu 
1               5                   10                  15      


Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
            20                  25                  30          


Pro 
    


<210>  76
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus Kozak sequence

<400>  76
gccrccatgg                                                              10


<210>  77
<211>  29
<212>  DNA
<213>  Homo sapiens

<400>  77
taacagttgc ttttatcaca ggctccagg                                         29


<210>  78
<211>  29
<212>  DNA
<213>  Homo sapiens

<400>  78
attctcaacg aaaatagtgt ccgaggtcc                                         29


<210>  79
<211>  293
<212>  PRT
<213>  Ophiostoma novo-ulmi subsp. americana (mitochondrion)

<400>  79

Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu Gly Ser 
1               5                   10                  15      


Phe Leu Leu Arg Ile Arg Asn Asn Asn Lys Ser Ser Val Gly Tyr Ser 
            20                  25                  30          


Thr Glu Leu Gly Phe Gln Ile Thr Leu His Asn Lys Asp Lys Ser Ile 
        35                  40                  45              


Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Val Ile Ala Asn Ser 
    50                  55                  60                  


Gly Asp Asn Ala Val Ser Leu Lys Val Thr Arg Phe Glu Asp Leu Lys 
65                  70                  75                  80  


Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys Leu 
                85                  90                  95      


Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Met Glu Asn Lys 
            100                 105                 110         


Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile Lys Ala 
        115                 120                 125             


Lys Leu Asn Trp Gly Leu Thr Asp Glu Leu Lys Lys Ala Phe Pro Glu 
    130                 135                 140                 


Asn Ile Ser Lys Glu Arg Ser Leu Ile Asn Lys Asn Ile Pro Asn Phe 
145                 150                 155                 160 


Lys Trp Leu Ala Gly Phe Thr Ser Gly Glu Gly Cys Phe Phe Val Asn 
                165                 170                 175     


Leu Ile Lys Ser Lys Ser Lys Leu Gly Val Gln Val Gln Leu Val Phe 
            180                 185                 190         


Ser Ile Thr Gln His Ile Lys Asp Lys Asn Leu Met Asn Ser Leu Ile 
        195                 200                 205             


Thr Tyr Leu Gly Cys Gly Tyr Ile Lys Glu Lys Asn Lys Ser Glu Phe 
    210                 215                 220                 


Ser Trp Leu Asp Phe Val Val Thr Lys Phe Ser Asp Ile Asn Asp Lys 
225                 230                 235                 240 


Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys Leu Glu 
                245                 250                 255     


Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu Lys Lys 
            260                 265                 270         


His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu Asn 
        275                 280                 285             


Met Asn Lys Gly Arg 
    290             


<210>  80
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant

<400>  80

Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu Gly Ser 
1               5                   10                  15      


Phe Val Leu Ser Ile Gln Asn Arg Asn Asp Tyr Ala Thr Gly Tyr Arg 
            20                  25                  30          


Ile His Leu Thr Phe Gln Ile Thr Leu His Asn Lys Asp Lys Ser Ile 
        35                  40                  45              


Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Lys Ile Asn Asn Ala 
    50                  55                  60                  


Gly Asp Asn Leu Val Gln Leu Arg Val Tyr Arg Phe Glu Asp Leu Lys 
65                  70                  75                  80  


Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys Leu 
                85                  90                  95      


Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Met Glu Asn Lys 
            100                 105                 110         


Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile Lys Ala 
        115                 120                 125             


Lys Met Asn Trp Gly Leu Asn Asp Glu Leu Lys Lys Ala Phe Pro Glu 
    130                 135                 140                 


Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro Asn Phe 
145                 150                 155                 160 


Lys Trp Leu Ala Gly Phe Thr Ser Gly Glu Gly Ser Phe Phe Val Arg 
                165                 170                 175     


Leu Arg Lys Ser Asn Val Asn Ala Arg Val Arg Val Gln Leu Val Phe 
            180                 185                 190         


Glu Ile Ser Gln His Ile Arg Asp Lys Asn Leu Met Asn Ser Leu Ile 
        195                 200                 205             


Thr Tyr Leu Gly Cys Gly His Ile Tyr Glu Gly Asn Lys Ser Glu Arg 
    210                 215                 220                 


Ser Trp Leu Gln Phe Arg Val Glu Lys Phe Ser Asp Ile Asn Asp Lys 
225                 230                 235                 240 


Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys Leu Glu 
                245                 250                 255     


Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu Lys Lys 
            260                 265                 270         


His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu Asn 
        275                 280                 285             


Met Asn Lys Gly Arg 
    290             


<210>  81
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI LHE variant

<400>  81

Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu Gly Ser 
1               5                   10                  15      


Phe Val Leu Ser Ile Gln Asn Arg Asn Asp Tyr Ala Thr Gly Tyr Arg 
            20                  25                  30          


Ile His Leu Thr Phe Gln Ile Thr Leu His Asn Lys Asp Lys Ser Ile 
        35                  40                  45              


Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Lys Ile Asn Asn Thr 
    50                  55                  60                  


Gly Asp Asn Leu Val Gln Leu Arg Val Tyr Arg Phe Glu Asp Leu Lys 
65                  70                  75                  80  


Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys Leu 
                85                  90                  95      


Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Met Glu Asn Lys 
            100                 105                 110         


Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile Lys Ala 
        115                 120                 125             


Lys Met Asn Trp Gly Leu Asn Asp Glu Leu Lys Lys Ala Phe Pro Glu 
    130                 135                 140                 


Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro Asn Phe 
145                 150                 155                 160 


Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser Phe Phe Val Arg 
                165                 170                 175     


Leu Arg Lys Ser Asn Val Asn Ala Arg Val Arg Val Gln Leu Val Phe 
            180                 185                 190         


Glu Ile Ser Gln His Ile Arg Asp Lys Asn Leu Met Asn Ser Leu Ile 
        195                 200                 205             


Thr Tyr Leu Gly Cys Gly His Ile Tyr Glu Gly Asn Lys Ser Glu Arg 
    210                 215                 220                 


Ser Trp Leu Gln Phe Arg Val Glu Lys Phe Ser Asp Ile Asn Asp Lys 
225                 230                 235                 240 


Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys Leu Glu 
                245                 250                 255     


Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu Lys Lys 
            260                 265                 270         


His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu Asn 
        275                 280                 285             


Met Asn Lys Gly Arg 
    290             


<210>  82
<211>  47
<212>  DNA
<213>  Homo sapiens

<400>  82
agctagtcta gtgcaagcta acagttgctt ttatcacagg ctccagg                     47


<210>  83
<211>  47
<212>  DNA
<213>  Homo sapiens

<400>  83
tcgatcagat cacgttcgat tctcaacgaa aatagtgtcc gaggtcc                     47


<210>  84
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  84
caaaccctcc tggagcctgt g                                                 21


<210>  85
<211>  28
<212>  DNA
<213>  Homo sapiens

<400>  85
caaaccctcc tggagcctgt ggggataa                                          28


<210>  86
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  86
caaaccctcc tggggcctgt g                                                 21


<210>  87
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  87
caaaccctcc tggagcccgt g                                                 21


<210>  88
<211>  18
<212>  DNA
<213>  Homo sapiens

<400>  88
caaaccctcc tggagccc                                                     18


<210>  89
<211>  12
<212>  DNA
<213>  Homo sapiens

<400>  89
caaaccctcc tg                                                           12


<210>  90
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  90
caaaccctcc tggagccggt                                                   20


<210>  91
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  91
caaaccctcc tggagcctgg                                                   20


<210>  92
<211>  19
<212>  DNA
<213>  Homo sapiens

<400>  92
caaaccctcc tggagcctg                                                    19


<210>  93
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  93
caaaccctcc tggagcctgt gg                                                22


<210>  94
<211>  25
<212>  DNA
<213>  Homo sapiens

<400>  94
aagcaactgt tagcttgcac tagac                                             25


<210>  95
<211>  25
<212>  DNA
<213>  Homo sapiens

<400>  95
aagcgactgt tagcttgcac tagac                                             25


<210>  96
<211>  31
<212>  DNA
<213>  Homo sapiens

<400>  96
ataaggaagc aactgttagc ttgcactaga c                                      31


<210>  97
<211>  26
<212>  DNA
<213>  Homo sapiens

<400>  97
aaagcaactg ttagcttgca ctagac                                            26


<210>  98
<211>  16
<212>  DNA
<213>  Homo sapiens

<400>  98
ttagcttgca ctagac                                                       16


<210>  99
<211>  15
<212>  DNA
<213>  Homo sapiens

<400>  99
tagcttgcac tagac                                                        15


<210>  100
<211>  18
<212>  DNA
<213>  Homo sapiens

<400>  100
tgttagcttg cactagac                                                     18


<210>  101
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  101
caactgttag cttgcactag ac                                                22


<210>  102
<211>  14
<212>  DNA
<213>  Homo sapiens

<400>  102
agcttgcact agac                                                         14


<210>  103
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  103
actgttagct tgcactagac                                                   20


<210>  104
<211>  25
<212>  DNA
<213>  Homo sapiens

<400>  104
aagcaactgt tagcttgcac tggac                                             25


