                         SEQUENCE LISTING

<110>  Editas Medicine, Inc.
       Maeder, Morgan L.
       Mepani, Rina J.
       Stefanidakis, Michael
 
<120>  Compositions and Methods for Treating CEP290-Associated Disease

<130>  118945.8012.WO00 (EM087PCT)

<150>  US 62/370,202
<151>  2016-08-02

<150>  US 62/400,526
<151>  2016-09-27

<150>  US 62/443,568
<151>  2017-01-06

<150>  US 62/503,800
<151>  2017-05-09

<150>  US 62/535,193
<151>  2017-07-20

<160>  39    

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  1
gttctgtcct cagtaaaagg ta                                                22


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  2
gaatagtttg ttctgggtac                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  3
gagaaaggga tgggcactta                                                   20


<210>  4
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  4
gtcaaaagct accggttacc tg                                                22


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  5
gatgcagaac tagtgtagac                                                   20


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  6
gagtatctcc tgtttggca                                                    19


<210>  7
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S. aureus gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Targeting domain of 16-24 nts, each n is a, c, t, g, unknown, or 
       other

<400>  7
nnnnnnnnnn nnnnnnnnnn gttttagtac tctggaaaca gaatctacta aaacaaggca       60

aaatgccgtg tttatctcgt caacttgttg gcgagatttt tt                         102


<210>  8
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S. aureus gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Targeting domain of 16-24 nts, each n is a, c, t, g, unknown, or 
       other

<400>  8
nnnnnnnnnn nnnnnnnnnn gttatagtac tctggaaaca gaatctacta taacaaggca       60

aaatgccgtg tttatctcgt caacttgttg gcgagatttt tt                         102


<210>  9
<211>  264
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U6 promoter

<400>  9
aaggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac       60

aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa      120

aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt      180

aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat      240

atcttgtgga aaggacgaaa cacc                                             264


<210>  10
<211>  3159
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized S. aureus Cas9

<400>  10
atgaaaagga actacattct ggggctggac atcgggatta caagcgtggg gtatgggatt       60

attgactatg aaacaaggga cgtgatcgac gcaggcgtca gactgttcaa ggaggccaac      120

gtggaaaaca atgagggacg gagaagcaag aggggagcca ggcgcctgaa acgacggaga      180

aggcacagaa tccagagggt gaagaaactg ctgttcgatt acaacctgct gaccgaccat      240

tctgagctga gtggaattaa tccttatgaa gccagggtga aaggcctgag tcagaagctg      300

tcagaggaag agttttccgc agctctgctg cacctggcta agcgccgagg agtgcataac      360

gtcaatgagg tggaagagga caccggcaac gagctgtcta caaaggaaca gatctcacgc      420

aatagcaaag ctctggaaga gaagtatgtc gcagagctgc agctggaacg gctgaagaaa      480

gatggcgagg tgagagggtc aattaatagg ttcaagacaa gcgactacgt caaagaagcc      540

aagcagctgc tgaaagtgca gaaggcttac caccagctgg atcagagctt catcgatact      600

tatatcgacc tgctggagac tcggagaacc tactatgagg gaccaggaga agggagcccc      660

ttcggatgga aagacatcaa ggaatggtac gagatgctga tgggacattg cacctatttt      720

ccagaagagc tgagaagcgt caagtacgct tataacgcag atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gaaaacgaga aactggaata ctatgagaag      840

ttccagatca tcgaaaacgt gtttaagcag aagaaaaagc ctacactgaa acagattgct      900

aaggagatcc tggtcaacga agaggacatc aagggctacc gggtgacaag cactggaaaa      960

ccagagttca ccaatctgaa agtgtatcac gatattaagg acatcacagc acggaaagaa     1020

atcattgaga acgccgaact gctggatcag attgctaaga tcctgactat ctaccagagc     1080

tccgaggaca tccaggaaga gctgactaac ctgaacagcg agctgaccca ggaagagatc     1140

gaacagatta gtaatctgaa ggggtacacc ggaacacaca acctgtccct gaaagctatc     1200

aatctgattc tggatgagct gtggcataca aacgacaatc agattgcaat ctttaaccgg     1260

ctgaagctgg tcccaaaaaa ggtggacctg agtcagcaga aagagatccc aaccacactg     1320

gtggacgatt tcattctgtc acccgtggtc aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcattatcga gctggctagg     1440

gagaagaaca gcaaggacgc acagaagatg atcaatgaga tgcagaaacg aaaccggcag     1500

accaatgaac gcattgaaga gattatccga actaccggga aagagaacgc aaagtacctg     1560

attgaaaaaa tcaagctgca cgatatgcag gagggaaagt gtctgtattc tctggaggcc     1620

atccccctgg aggacctgct gaacaatcca ttcaactacg aggtcgatca tattatcccc     1680

agaagcgtgt ccttcgacaa ttcctttaac aacaaggtgc tggtcaagca ggaagagaac     1740

tctaaaaagg gcaataggac tcctttccag tacctgtcta gttcagattc caagatctct     1800

tacgaaacct ttaaaaagca cattctgaat ctggccaaag gaaagggccg catcagcaag     1860

accaaaaagg agtacctgct ggaagagcgg gacatcaaca gattctccgt ccagaaggat     1920

tttattaacc ggaatctggt ggacacaaga tacgctactc gcggcctgat gaatctgctg     1980

cgatcctatt tccgggtgaa caatctggat gtgaaagtca agtccatcaa cggcgggttc     2040

acatcttttc tgaggcgcaa atggaagttt aaaaaggagc gcaacaaagg gtacaagcac     2100

catgccgaag atgctctgat tatcgcaaat gccgacttca tctttaagga gtggaaaaag     2160

ctggacaaag ccaagaaagt gatggagaac cagatgttcg aagagaagca ggccgaatct     2220

atgcccgaaa tcgagacaga acaggagtac aaggagattt tcatcactcc tcaccagatc     2280

aagcatatca aggatttcaa ggactacaag tactctcacc gggtggataa aaagcccaac     2340

agagagctga tcaatgacac cctgtatagt acaagaaaag acgataaggg gaataccctg     2400

attgtgaaca atctgaacgg actgtacgac aaagataatg acaagctgaa aaagctgatc     2460

aacaaaagtc ccgagaagct gctgatgtac caccatgatc ctcagacata tcagaaactg     2520

aagctgatta tggagcagta cggcgacgag aagaacccac tgtataagta ctatgaagag     2580

actgggaact acctgaccaa gtatagcaaa aaggataatg gccccgtgat caagaagatc     2640

aagtactatg ggaacaagct gaatgcccat ctggacatca cagacgatta ccctaacagt     2700

cgcaacaagg tggtcaagct gtcactgaag ccatacagat tcgatgtcta tctggacaac     2760

ggcgtgtata aatttgtgac tgtcaagaat ctggatgtca tcaaaaagga gaactactat     2820

gaagtgaata gcaagtgcta cgaagaggct aaaaagctga aaaagattag caaccaggca     2880

gagttcatcg cctcctttta caacaacgac ctgattaaga tcaatggcga actgtatagg     2940

gtcatcgggg tgaacaatga tctgctgaac cgcattgaag tgaatatgat tgacatcact     3000

taccgagagt atctggaaaa catgaatgat aagcgccccc ctcgaattat caaaacaatt     3060

gcctctaaga ctcagagtat caaaaagtac tcaaccgaca ttctgggaaa cctgtatgag     3120

gtgaagagca aaaagcaccc tcagattatc aaaaagggc                            3159


<210>  11
<211>  1053
<212>  PRT
<213>  Staphylococcus aureus

<400>  11

Met Lys Arg Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile 
    50                  55                  60                  


Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His 
65                  70                  75                  80  


Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr 
        115                 120                 125             


Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala 
    130                 135                 140                 


Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys 
145                 150                 155                 160 


Asp Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr 
                165                 170                 175     


Val Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln 
            180                 185                 190         


Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg 
        195                 200                 205             


Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys 
    210                 215                 220                 


Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe 
225                 230                 235                 240 


Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr 
                245                 250                 255     


Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn 
            260                 265                 270         


Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe 
        275                 280                 285             


Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu 
    290                 295                 300                 


Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys 
305                 310                 315                 320 


Pro Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr 
                325                 330                 335     


Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala 
            340                 345                 350         


Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu 
        355                 360                 365             


Thr Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser 
    370                 375                 380                 


Asn Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile 
385                 390                 395                 400 


Asn Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala 
                405                 410                 415     


Ile Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln 
            420                 425                 430         


Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro 
        435                 440                 445             


Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile 
    450                 455                 460                 


Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg 
465                 470                 475                 480 


Glu Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys 
                485                 490                 495     


Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr 
            500                 505                 510         


Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp 
        515                 520                 525             


Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu 
    530                 535                 540                 


Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro 
545                 550                 555                 560 


Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys 
                565                 570                 575     


Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu 
            580                 585                 590         


Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile 
        595                 600                 605             


Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu 
    610                 615                 620                 


Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp 
625                 630                 635                 640 


Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu 
                645                 650                 655     


Met Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys 
            660                 665                 670         


Val Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp 
        675                 680                 685             


Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp 
    690                 695                 700                 


Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys 
705                 710                 715                 720 


Leu Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys 
                725                 730                 735     


Gln Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu 
            740                 745                 750         


Ile Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp 
        755                 760                 765             


Tyr Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu 
785                 790                 795                 800 


Ile Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu 
                805                 810                 815     


Lys Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His 
            820                 825                 830         


Asp Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly 
        835                 840                 845             


Asp Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr 
    850                 855                 860                 


Leu Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile 
865                 870                 875                 880 


Lys Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp 
                885                 890                 895     


Tyr Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr 
            900                 905                 910         


Arg Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val 
        915                 920                 925             


Lys Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser 
    930                 935                 940                 


Lys Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys Ile Ser Asn Gln Ala 
945                 950                 955                 960 


Glu Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Ile Asn Gly 
                965                 970                 975     


Glu Leu Tyr Arg Val Ile Gly Val Asn Asn Asp Leu Leu Asn Arg Ile 
            980                 985                 990         


Glu Val Asn Met Ile Asp Ile Thr  Tyr Arg Glu Tyr Leu  Glu Asn Met 
        995                 1000                 1005             


Asn Asp  Lys Arg Pro Pro Arg  Ile Ile Lys Thr Ile  Ala Ser Lys 
    1010                 1015                 1020             


Thr Gln  Ser Ile Lys Lys Tyr  Ser Thr Asp Ile Leu  Gly Asn Leu 
    1025                 1030                 1035             


Tyr Glu  Val Lys Ser Lys Lys  His Pro Gln Ile Ile  Lys Lys Gly 
    1040                 1045                 1050             


<210>  12
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polyadenylation signal

<400>  12
tagcaataaa ggatcgttta ttttcattgg aagcgtgtgt tggttttttg atcaggcgcg       60


<210>  13
<211>  617
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CMV promoter

<400>  13
cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca       60

tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac      120

gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact      180

ttccattgac gtcaatgggt ggactattta cggtaaactg cccacttggc agtacatcaa      240

gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg      300

cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta      360

gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg      420

tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg      480

caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg      540

ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag      600

atccgctaga gatccgc                                                     617


<210>  14
<211>  252
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EFS promoter

<400>  14
tcgagtggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag       60

ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg      120

gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata      180

agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggtg      240

tcgtgaccgc gg                                                          252


<210>  15
<211>  292
<212>  DNA
<213>  Homo sapiens

<400>  15
gggccccaga agcctggtgg ttgtttgtcc ttctcagggg aaaagtgagg cggccccttg       60

gaggaagggg ccgggcagaa tgatctaatc ggattccaag cagctcaggg gattgtcttt      120

ttctagcacc ttcttgccac tcctaagcgt cctccgtgac cccggctggg atttcgcctg      180

gtgctgtgtc agccccggtc tcccaggggc ttcccagtgg tccccaggaa ccctcgacag      240

ggcccggtct ctctcgtcca gcaagggcag ggacgggcca caggccaagg gc              292


<210>  16
<211>  145
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAV2 left ITR sequence

<400>  16
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc       60

cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg      120

gccaactcca tcactagggg ttcct                                            145


<210>  17
<211>  145
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAV2 right ITR sequence

<400>  17
aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg       60

ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca gtgagcgagc      120

gagcgcgcag agagggagtg gccaa                                            145


<210>  18
<211>  36
<212>  DNA
<213>  Mus musculus

<400>  18
ccctcaaaca catgtctcac gcagcttaga cattct                                 36


<210>  19
<211>  35
<212>  DNA
<213>  Mus musculus

<400>  19
cagaactcgg tcagcatgct acagatagct tatct                                  35


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C1 targeting domain

<400>  20
ggccggctaa tttagtagag a                                                 21


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C2 targeting domain

<400>  21
gttatgaaga ataatacaaa                                                   20


<210>  22
<211>  5120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reporter construct pAD26/SplitGFP+WildType/CEP290/Kan

<400>  22
acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg       60

tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg      120

gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt      180

acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg      240

accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg      300

gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt      360

ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac      420

tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg      480

tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg      540

gccgctaata cgactcacta tagggagagc cgccaccatg gtgagcaagg gcgaggagct      600

gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt      660

cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat      720

ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg      780

aggtaagttt gtgtgattct tgaaccttgt gaaattagcc atttttcttc aatatttttg      840

tgtttggggg gatttggcag attttaatta aagtttgcct gcatttatat aaatttaaca      900

gagatataat tatccatatt attcattgtg gctgaatgac ttctgaatga ttatctagat      960

cattctcctt aggtcacttg catgatttag ctgaatcaaa cctcttttaa ccagacatct     1020

aagagaaaaa ggagcatgaa acaggtagaa tattgtaatc aaaggaggga agcactcatt     1080

aagtgcccat ccctttctct tacccctgta cccagaacaa actattctcc catggtccct     1140

ggcttttgtt ccttggaatg gatgtagcca acagtagctg aaatattaag ggctcttcct     1200

ggaccatgga tgcactctgt aaattctcat cattttttat tgtagaataa atgtagaatt     1260

ttaatgtaga ataaatttat ttaatgtaga ataaaaaata aaaaaactag agtagaatat     1320

cataagttac aatctgtgaa tatggaccag accctttgta gttatcttac agccacttga     1380

actctatacc ttttactgag gacagaacaa gctcctgatt tgttcatctt cctcatcaga     1440

aatagaggct tatggatttt ggattattct tatctaagat cctttcacag gagtagaata     1500

agatctaatt ctattagctc aaaagctttt gctggctcat agagacacat tcagtaaatg     1560

aaaacgttgt tctgagtagc tttcaggatt cctactaaat tatgagtcat gtttatcaat     1620

attatttaga agtaatcata atcagtttgc tttctgctgc ttttgccaaa gagaggtgat     1680

tatgttactt tttatagaaa attatgccta tttagtgtgg tgataattta tttttttcca     1740

ttctccatgt cctctgtcct atcctctcca gcattagaaa gtcctaggca agagacatct     1800

tgtggataat gtatcaatga gtgatgttta acgttatcat tttcccaaag agtatttttc     1860

atctttccta aagatttttt tttttttttt ttgagatgga gtttcattct gtcacccagg     1920

ctgagtgcag tggcacgatc tcggcttaac gcttactgca tcctctgcct cccagattca     1980

agcagttctc ctgcctcagc ctctgagtag ctgggattac aggtgtgcac caccacacca     2040

gctaattttt tttttttttt tttttttttt gaggcagagt ctcgctctgt cacccaggct     2100

ggagtgcagt ggcgccatct tggctcactg caagctccac ctcccgggtt caggccgttc     2160

tcctgcctca gcctcctgag tagctggtac cacaggcacc caccatcatg cccggctaat     2220

tttttgtatt tttagtagag atggggtttc accttgttag ccaggatggt gtcgatctcc     2280

tgaactcgtg atccacccgc ctcggcctcc taaagtgctg ggattacaga tgtgagccac     2340

cgcacctggc cccagttgta attgtgaata tctcatacct atccctattg gcagtgtctt     2400

agttttattt tttattatct ttattgtggc agccattatt cctgtctcta tctccagtct     2460

tacatcctcc ttactgccac aagaatgatc attctaaaca tgaatcctac cctgtgactc     2520

ccatgtgact ccccgcctta aaaactgtca aaagctaccg gttacctgaa gggtaaaagt     2580

caagtcccct acttacctca tgtcatctag agcaagagat gaactagctg agttttctga     2640

ccacagtgtt ctttcttatg tatgttcttt tgtacgtgct cttttctata tatagggaac     2700

catttctctc ttccagttgt tttgctcagt gaatttctat tcctgtttca aaacttgttc     2760

aggcattacc ttttttttct taagcatact ttttttaatg gaacaaagtc actcctgtct     2820

acactagttc tgcatcttat acataggttt tgtacatagt acatatttat atcacatcaa     2880

attatatgtg tttacatatc tgtcttcctt aatggaatat aagtcttttg atataaggaa     2940

ctatttaatt tgtttctgtg tgttgagtat ctcctgtttg gcacagagtt caagctaata     3000

catgagagtg attagtggtg gagagccaca gtgcatgtgg tgtcaaatat ggtgcttagg     3060

aaattattgt tgctttttga gaggtaaagg ttcatgagac tagaggtcac gaaaatcaga     3120

tttcatgtgt gaagaatgga atagatgtat tatatatgga ggaaattcta attttgtaaa     3180

aaactggatt gtgagtttta aggagatgtt atataaagtt aagacaatgt cattttgtgg     3240

tattggtctg aattacaatg tagtttctta gtgatatttt tcctttattc agtgcagtgc     3300

ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa     3360

ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc     3420

gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc     3480

aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc     3540

tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac     3600

atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac     3660

ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac     3720

cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact     3780

ctcggcatgg acgagctgta caagtcgagc tagcaataaa ggatcgttta ttttcattgg     3840

aagcgtgtgt tggttttttg atcaggcgcg tccaagcttg catgctgggg agagatctgc     3900

ggccgctcga gtggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc     3960

gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta     4020

aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg     4080

tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca     4140

caggtgtcgt gaccgcgggc ggccgccgct agcgctaccg gtcgccacca tggtgagcaa     4200

gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga     4260

gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga     4320

gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga     4380

catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat     4440

ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt     4500

cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat     4560

ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa     4620

gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg     4680

cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac     4740

cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt     4800

ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg     4860

ccgccactcc accggcggca tggacgagct gtacaagtga ccggtcatca tcaccatcac     4920

cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt     4980

gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc     5040

taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt     5100

ggggtggggc aggacagcaa                                                 5120


<210>  23
<211>  5120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reporter construct pAD27/SplitGFP+Mutant/CEP290/Kan

<400>  23
acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg       60

tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg      120

gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt      180

acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg      240

accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg      300

gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt      360

ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac      420

tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg      480

tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg      540

gccgctaata cgactcacta tagggagagc cgccaccatg gtgagcaagg gcgaggagct      600

gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt      660

cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat      720

ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg      780

aggtaagttt gtgtgattct tgaaccttgt gaaattagcc atttttcttc aatatttttg      840

tgtttggggg gatttggcag attttaatta aagtttgcct gcatttatat aaatttaaca      900

gagatataat tatccatatt attcattgtg gctgaatgac ttctgaatga ttatctagat      960

cattctcctt aggtcacttg catgatttag ctgaatcaaa cctcttttaa ccagacatct     1020

aagagaaaaa ggagcatgaa acaggtagaa tattgtaatc aaaggaggga agcactcatt     1080

aagtgcccat ccctttctct tacccctgta cccagaacaa actattctcc catggtccct     1140

ggcttttgtt ccttggaatg gatgtagcca acagtagctg aaatattaag ggctcttcct     1200

ggaccatgga tgcactctgt aaattctcat cattttttat tgtagaataa atgtagaatt     1260

ttaatgtaga ataaatttat ttaatgtaga ataaaaaata aaaaaactag agtagaatat     1320

cataagttac aatctgtgaa tatggaccag accctttgta gttatcttac agccacttga     1380

actctatacc ttttactgag gacagaacaa gctcctgatt tgttcatctt cctcatcaga     1440

aatagaggct tatggatttt ggattattct tatctaagat cctttcacag gagtagaata     1500

agatctaatt ctattagctc aaaagctttt gctggctcat agagacacat tcagtaaatg     1560

aaaacgttgt tctgagtagc tttcaggatt cctactaaat tatgagtcat gtttatcaat     1620

attatttaga agtaatcata atcagtttgc tttctgctgc ttttgccaaa gagaggtgat     1680

tatgttactt tttatagaaa attatgccta tttagtgtgg tgataattta tttttttcca     1740

ttctccatgt cctctgtcct atcctctcca gcattagaaa gtcctaggca agagacatct     1800

tgtggataat gtatcaatga gtgatgttta acgttatcat tttcccaaag agtatttttc     1860

atctttccta aagatttttt tttttttttt ttgagatgga gtttcattct gtcacccagg     1920

ctgagtgcag tggcacgatc tcggcttaac gcttactgca tcctctgcct cccagattca     1980

agcagttctc ctgcctcagc ctctgagtag ctgggattac aggtgtgcac caccacacca     2040

gctaattttt tttttttttt tttttttttt gaggcagagt ctcgctctgt cacccaggct     2100

ggagtgcagt ggcgccatct tggctcactg caagctccac ctcccgggtt caggccgttc     2160

tcctgcctca gcctcctgag tagctggtac cacaggcacc caccatcatg cccggctaat     2220

tttttgtatt tttagtagag atggggtttc accttgttag ccaggatggt gtcgatctcc     2280

tgaactcgtg atccacccgc ctcggcctcc taaagtgctg ggattacaga tgtgagccac     2340

cgcacctggc cccagttgta attgtgagta tctcatacct atccctattg gcagtgtctt     2400

agttttattt tttattatct ttattgtggc agccattatt cctgtctcta tctccagtct     2460

tacatcctcc ttactgccac aagaatgatc attctaaaca tgaatcctac cctgtgactc     2520

ccatgtgact ccccgcctta aaaactgtca aaagctaccg gttacctgaa gggtaaaagt     2580

caagtcccct acttacctca tgtcatctaa agcaagagat gaactagctg agttttctga     2640

ccacagtgtt ctttcttatg tatgttcttt tgtacgtgct cttttctata tatagggaac     2700

catttctctc ttccagttgt tttgctcagt gaatttctat tcctgtttca aaacttgttc     2760

aggcattacc ttttttttct taagcatact ttttttaatg gaacaaagtc actcctgtct     2820

acactagttc tgcatcttat acataggttt tgtacatagt acatatttat atcacatcaa     2880

attatatgtg tttacatatc tgtcttcctt aatggaatat aagtcttttg atataaggaa     2940

ctatttaatt tgtttctgtg tgttgagtat ctcctgtttg gcacagagtt caagctaata     3000

catgagagtg attagtggtg gagagccaca gtgcatgtgg tgtcaaatat ggtgcttagg     3060

aaattattgt tgctttttga gaggtaaagg ttcatgagac tagaggtcac gaaaatcaga     3120

tttcatgtgt gaagaatgga atagatgtat tatatatgga ggaaattcta attttgtaaa     3180

aaactggatt gtgagtttta aggagatgtt atataaagtt aagacaatgt cattttgtgg     3240

tattggtctg aattacaatg tagtttctta gtgatatttt tcctttattc agtgcagtgc     3300

ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa     3360

ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc     3420

gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc     3480

aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc     3540

tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac     3600

atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac     3660

ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac     3720

cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact     3780

ctcggcatgg acgagctgta caagtcgagc tagcaataaa ggatcgttta ttttcattgg     3840

aagcgtgtgt tggttttttg atcaggcgcg tccaagcttg catgctgggg agagatctgc     3900

ggccgctcga gtggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc     3960

gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta     4020

aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg     4080

tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca     4140

caggtgtcgt gaccgcgggc ggccgccgct agcgctaccg gtcgccacca tggtgagcaa     4200

gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga     4260

gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga     4320

gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga     4380

catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat     4440

ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt     4500

cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat     4560

ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa     4620

gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg     4680

cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac     4740

cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt     4800

ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg     4860

ccgccactcc accggcggca tggacgagct gtacaagtga ccggtcatca tcaccatcac     4920

cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt     4980

gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc     5040

taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt     5100

ggggtggggc aggacagcaa                                                 5120


<210>  24
<211>  5120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reporter construct pAD28/SplitGFP+Mutant/CEP290/Inverted/Kan

<400>  24
acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg       60

tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg      120

gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt      180

acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg      240

accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg      300

gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt      360

ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac      420

tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg      480

tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg      540

gccgctaata cgactcacta tagggagagc cgccaccatg gtgagcaagg gcgaggagct      600

gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt      660

cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat      720

ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg      780

aggtaagttt gtgtgattct tgaaccttgt gaaattagcc atttttcttc aatatttttg      840

tgtttggggg gatttggcag attttaatta aagtttgcct gcatttatat aaatttaaca      900

gagatataat tatccatatt attcattgtg gctgaatgac ttctgaatga ttatctagat      960

cattctcctt aggtcacttg catgatttag ctgaatcaaa cctcttttaa ccagacatct     1020

aagagaaaaa ggagcatgaa acaggtagaa tattgtaatc aaaggaggga agcactcatt     1080

aagtgcccat ccctttctct tacccctgta cccagaacaa actattctcc catggtccct     1140

ggcttttgtt ccttggaatg gatgtagcca acagtagctg aaatattaag ggctcttcct     1200

ggaccatgga tgcactctgt aaattctcat cattttttat tgtagaataa atgtagaatt     1260

ttaatgtaga ataaatttat ttaatgtaga ataaaaaata aaaaaactag agtagaatat     1320

cataagttac aatctgtgaa tatggaccag accctttgta gttatcttac agccacttga     1380

actctatacg taaccggtag cttttgacag tttttaaggc ggggagtcac atgggagtca     1440

cagggtagga ttcatgttta gaatgatcat tcttgtggca gtaaggagga tgtaagactg     1500

gagatagaga caggaataat ggctgccaca ataaagataa taaaaaataa aactaagaca     1560

ctgccaatag ggataggtat gagatactca caattacaac tggggccagg tgcggtggct     1620

cacatctgta atcccagcac tttaggaggc cgaggcgggt ggatcacgag ttcaggagat     1680

cgacaccatc ctggctaaca aggtgaaacc ccatctctac taaaaataca aaaaattagc     1740

cgggcatgat ggtgggtgcc tgtggtacca gctactcagg aggctgaggc aggagaacgg     1800

cctgaacccg ggaggtggag cttgcagtga gccaagatgg cgccactgca ctccagcctg     1860

ggtgacagag cgagactctg cctcaaaaaa aaaaaaaaaa aaaaaaaaat tagctggtgt     1920

ggtggtgcac acctgtaatc ccagctactc agaggctgag gcaggagaac tgcttgaatc     1980

tgggaggcag aggatgcagt aagcgttaag ccgagatcgt gccactgcac tcagcctggg     2040

tgacagaatg aaactccatc tcaaaaaaaa aaaaaaaaaa tctttaggaa agatgaaaaa     2100

tactctttgg gaaaatgata acgttaaaca tcactcattg atacattatc cacaagatgt     2160

ctcttgccta ggactttcta atgctggaga ggataggaca gaggacatgg agaatggaaa     2220

aaaataaatt atcaccacac taaataggca taattttcta taaaaagtaa cataatcacc     2280

tctctttggc aaaagcagca gaaagcaaac tgattatgat tacttctaaa taatattgat     2340

aaacatgact cataatttag taggaatcct gaaagctact cagaacaacg ttttcattta     2400

ctgaatgtgt ctctatgagc cagcaaaagc ttttgagcta atagaattag atcttattct     2460

actcctgtga aaggatctta gataagaata atccaaaatc cataagcctc tatttctgat     2520

gaggaagatg aacaaatcag gagcttgttc tgtcctcagt aaaagctgaa gggtaaaagt     2580

caagtcccct acttacctca tgtcatctaa agcaagagat gaactagctg agttttctga     2640

ccacagtgtt ctttcttatg tatgttcttt tgtacgtgct cttttctata tatagggaac     2700

catttctctc ttccagttgt tttgctcagt gaatttctat tcctgtttca aaacttgttc     2760

aggcattacc ttttttttct taagcatact ttttttaatg gaacaaagtc actcctgtct     2820

acactagttc tgcatcttat acataggttt tgtacatagt acatatttat atcacatcaa     2880

attatatgtg tttacatatc tgtcttcctt aatggaatat aagtcttttg atataaggaa     2940

ctatttaatt tgtttctgtg tgttgagtat ctcctgtttg gcacagagtt caagctaata     3000

catgagagtg attagtggtg gagagccaca gtgcatgtgg tgtcaaatat ggtgcttagg     3060

aaattattgt tgctttttga gaggtaaagg ttcatgagac tagaggtcac gaaaatcaga     3120

tttcatgtgt gaagaatgga atagatgtat tatatatgga ggaaattcta attttgtaaa     3180

aaactggatt gtgagtttta aggagatgtt atataaagtt aagacaatgt cattttgtgg     3240

tattggtctg aattacaatg tagtttctta gtgatatttt tcctttattc agtgcagtgc     3300

ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa     3360

ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc     3420

gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc     3480

aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc     3540

tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac     3600

atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac     3660

ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac     3720

cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact     3780

ctcggcatgg acgagctgta caagtcgagc tagcaataaa ggatcgttta ttttcattgg     3840

aagcgtgtgt tggttttttg atcaggcgcg tccaagcttg catgctgggg agagatctgc     3900

ggccgctcga gtggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc     3960

gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta     4020

aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg     4080

tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca     4140

caggtgtcgt gaccgcgggc ggccgccgct agcgctaccg gtcgccacca tggtgagcaa     4200

gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga     4260

gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga     4320

gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga     4380

catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat     4440

ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt     4500

cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat     4560

ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa     4620

gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg     4680

cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac     4740

cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt     4800

ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg     4860

ccgccactcc accggcggca tggacgagct gtacaagtga ccggtcatca tcaccatcac     4920

cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt     4980

gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc     5040

taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt     5100

ggggtggggc aggacagcaa                                                 5120


<210>  25
<211>  3944
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reporter construct pAD29/SplitGFP+Deletion/CEP290/Kan

<400>  25
acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg       60

tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg      120

gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt      180

acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg      240

accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg      300

gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt      360

ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac      420

tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg      480

tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg      540

gccgctaata cgactcacta tagggagagc cgccaccatg gtgagcaagg gcgaggagct      600

gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt      660

cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat      720

ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg      780

aggtaagttt gtgtgattct tgaaccttgt gaaattagcc atttttcttc aatatttttg      840

tgtttggggg gatttggcag attttaatta aagtttgcct gcatttatat aaatttaaca      900

gagatataat tatccatatt attcattgtg gctgaatgac ttctgaatga ttatctagat      960

cattctcctt aggtcacttg catgatttag ctgaatcaaa cctcttttaa ccagacatct     1020

aagagaaaaa ggagcatgaa acaggtagaa tattgtaatc aaaggaggga agcactcatt     1080

aagtgcccat ccctttctct tacccctgta cccagaacaa actattctcc catggtccct     1140

ggcttttgtt ccttggaatg gatgtagcca acagtagctg aaatattaag ggctcttcct     1200

ggaccatgga tgcactctgt aaattctcat cattttttat tgtagaataa atgtagaatt     1260

ttaatgtaga ataaatttat ttaatgtaga ataaaaaata aaaaaactag agtagaatat     1320

cataagttac aatctgtgaa tatggaccag accctttgta gttatcttac agccacttga     1380

actctatacc tgaagggtaa aagtcaagtc ccctacttac ctcatgtcat ctaaagcaag     1440

agatgaacta gctgagtttt ctgaccacag tgttctttct tatgtatgtt cttttgtacg     1500

tgctcttttc tatatatagg gaaccatttc tctcttccag ttgttttgct cagtgaattt     1560

ctattcctgt ttcaaaactt gttcaggcat tacctttttt ttcttaagca tacttttttt     1620

aatggaacaa agtcactcct gtctacacta gttctgcatc ttatacatag gttttgtaca     1680

tagtacatat ttatatcaca tcaaattata tgtgtttaca tatctgtctt ccttaatgga     1740

atataagtct tttgatataa ggaactattt aatttgtttc tgtgtgttga gtatctcctg     1800

tttggcacag agttcaagct aatacatgag agtgattagt ggtggagagc cacagtgcat     1860

gtggtgtcaa atatggtgct taggaaatta ttgttgcttt ttgagaggta aaggttcatg     1920

agactagagg tcacgaaaat cagatttcat gtgtgaagaa tggaatagat gtattatata     1980

tggaggaaat tctaattttg taaaaaactg gattgtgagt tttaaggaga tgttatataa     2040

agttaagaca atgtcatttt gtggtattgg tctgaattac aatgtagttt cttagtgata     2100

tttttccttt attcagtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac     2160

ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac     2220

gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc     2280

atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag     2340

tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag     2400

gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac     2460

cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc     2520

acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag     2580

ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc gagctagcaa     2640

taaaggatcg tttattttca ttggaagcgt gtgttggttt tttgatcagg cgcgtccaag     2700

cttgcatgct ggggagagat ctgcggccgc tcgagtggct ccggtgcccg tcagtgggca     2760

gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt     2820

gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt     2880

tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt     2940

cgcaacgggt ttgccgccag aacacaggtg tcgtgaccgc gggcggccgc cgctagcgct     3000

accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt     3060

catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg     3120

cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg     3180

tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc     3240

ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt     3300

caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc     3360

ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc     3420

cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta     3480

ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg     3540

ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg     3600

cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt     3660

ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa     3720

gtgaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg     3780

ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa     3840

ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt     3900

aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaa                      3944


<210>  26
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  26
guucuguccu caguaaaagg ua                                                22


<210>  27
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  27
gaauaguuug uucuggguac                                                   20


<210>  28
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  28
gagaaaggga ugggcacuua                                                   20


<210>  29
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  29
gucaaaagcu accgguuacc ug                                                22


<210>  30
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  30
gaugcagaac uaguguagac                                                   20


<210>  31
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  31
gaguaucucc uguuuggca                                                    19


<210>  32
<211>  102
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S. aureus gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Targeting domain of 16-24 nts, each n is a, c, u, g, unknown, or 
       other

<400>  32
nnnnnnnnnn nnnnnnnnnn guuuuaguac ucuggaaaca gaaucuacua aaacaaggca       60

aaaugccgug uuuaucucgu caacuuguug gcgagauuuu uu                         102


<210>  33
<211>  102
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S. aureus gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Targeting domain of 16-24 nts, each n is a, c, u, g, unknown, or 
       other

<400>  33
nnnnnnnnnn nnnnnnnnnn guuauaguac ucuggaaaca gaaucuacua uaacaaggca       60

aaaugccgug uuuaucucgu caacuuguug gcgagauuuu uu                         102


<210>  34
<211>  36
<212>  DNA
<213>  Mus musculus

<400>  34
cagaactcgg tcaggcatgc tacagatagc ttatct                                 36


<210>  35
<211>  31
<212>  DNA
<213>  Mus musculus

<400>  35
cagaactcgg catgctacag atagcttatc t                                      31


<210>  36
<211>  9
<212>  DNA
<213>  Mus musculus

<400>  36
ccctcaaag                                                                9


<210>  37
<211>  21
<212>  DNA
<213>  Mus musculus

<400>  37
catgctacag atagcttatc t                                                 21


<210>  38
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  C1 targeting domain

<400>  38
ggccggcuaa uuuaguagag a                                                 21


<210>  39
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  C2 targeting domain

<400>  39
guuaugaaga auaauacaaa                                                   20


