                         SEQUENCE LISTING

<110>  AniCon Labor GmbH
 
<120>  Verfahren zum Nachweis einer neuen Variante des IB-Virus sowie 
       Kit hierfr

<130>  AA 2428-02WO

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  3486
<212>  DNA
<213>  Avian infectious bronchitis virus

<400>  1
atgtcggtac tattactgtt aggactgact cttttgtctg ctttatgtga gtgtagtata       60

gtaggtgaca attacactta ctattaccac agcgaatata gacctcctaa tggttggcat      120

caacaaggcg gagcttatcg tgttattaat actacttccc ttagtgtgtc tacttgttct      180

ttaggtggtg tacctcctct actaactgta aatgcaccat ctgttactat agtagcaccc      240

actaagttgc cttggtctaa agctaatttt acgacagctt tttgccattt tggtaatttt      300

tctgtgtttg taactcattg tactactggc aactctcgga attgtacatt aacaccacct      360

attttagcag gttatattgt agttggtgct aatggttcaa ttacatataa ttacacacta      420

agagcgattg gtgatttttc acactttcat tcttttcagt gtgttagtaa ttttacttct      480

gtgtacttaa atgggaaact agtttttacg tctaaccttt cacttggtgt tttaagtgta      540

ggtgcatgtt taaatgcgac tggcgcagag ctcaagtttt ctgagtattt taatgtctta      600

gcttacttta taaatggtac agcacatgat gttattttat gcgatgatag ccctaagggg      660

gttttggctt gccagtataa tactggtaat tttacagatg gtttttatcc aatttctgtt      720

agtactaagg ctgatgatgt ttttaatgtc tatagaacta actctgtttt aagttatggt      780

tacttacgta acattacact aagtaattgt actgatgtta aacctaattt agggggtgaa      840

ctaacactct gtcaacaata tagtgtttcg gaaggttatg ttaattttaa tttttctttt      900

ttggatagtt ttacatatgt aggaccagat ctttataatt ttgactatgg ttctagtcat      960

tcaaattgtc attttacacc taatactatt aataatcggt tgtcgtttaa tactattagt     1020

gttgctatag gttatggtcc aatagatggt gcctgtaagc aaattagatt taggaatggt     1080

tttaacccat ctcatacttg ctgttatgct tacagacacc catccttaag tcatagttgt     1140

catggagtta ctacaggtaa gtatagtaat agttatgagt gtattcttat ggttgttatt     1200

agtagggatt atgggtctcg tgtgcagaca caaacagagg tacctactag tttaacttat     1260

ttttataata atattacttt ggatgtgtgt gttgattata atatttatgg tacattaggc     1320

caaggttatg ttactaatat aactgatatg gccttatccg gtggttactt gggttttggt     1380

ggattagtca tcctagactc gtctggagct attgacattt ttcttgtaga aagtgaaaat     1440

ggtcctaatt attataaaat taatccatgc aatgatgtca atcagcaata tgtagtgtca     1500

gggggtagta tagttggact cttaacatct aataatttaa ctggttctga attattagaa     1560

gatcagtttt atgttaaatt cactaataat gttcataggc gtagaaggtc tattagtgaa     1620

aatgttacat cttgtccata tccaacttat ggaaagtttt gtattaaacc agatggtagt     1680

gttagcacta tagtgccaga agaagttaaa gactatactt ctcttttgct taataggact     1740

gattatgtgc tcataccaga cagttttaat ttaactgtta cagatgagtt catacagaca     1800

caaatgcagc aggttcagat taattgcatt cagtatgtgt gtggtaattc tatacaatgc     1860

aagcaacttt tccgacagta tggtcctgta tgtgatagca tactttctat agttaatggc     1920

atagtacaac aagataatgc tgaaatactc cagttttact cttctactaa gcctaaaggg     1980

tttgatgaaa gctcttttag taattttaca gctggtgagt ttaatatttc gcttgtatta     2040

cctaagaatg gacgacccac agggcgttcg tttatagagg acttgttgtt tgacaaagta     2100

gaatctttag gactacctgg tgactcaact taccaaaaat gtacgtctgg ccccctaggt     2160

tttgttaaag atctagtgtg tgcgcagaag tataatggat tacttgtgtt gccacctatt     2220

ataacggctg aaatgcaagc aatgtatact agttcattag tagcctcaat ggcttttggt     2280

gggataacag ctgcaggagc aatacctttt gcaactcaat tgcaagcacg tataaaccat     2340

cttggtatta ctcagacggt tttgcaaaag aatcaggaga aaattgctgc atcatttaat     2400

aaagctatgg tgcatatgca ggatggattt aaagccacat ctcttgcatt acaacaagtg     2460

caagatgttg ttaacgagca tggtgcaata cttcagcaga ctatgcattc acttaataag     2520

aattttggtg ccataacaca tgtcatccaa gatatatata aacaactaga tgcattagaa     2580

gcaaatgcac aagttgatcg tttaattaat gggagacttt cgtctctatc tgtattagct     2640

tcttctaaac agttagagta tacaaaagtt tcgcatcaac gcgaattagc taaagaaaag     2700

attaatgagt gtgtaaaatc acaatctagc aggtatggct tctgtggtga tggcatgcat     2760

ataatgtcca tcccccagag tgcacctaat gggattgtgt tcttgcattt cacctacaca     2820

cctgtatcct ttcaaaatgt gacagcagtt gtgggttttt gtgttaaacc tgttaatggt     2880

agtgaatacg gccttgtgcc tgttgttggt agaggtattt ttatagaagt aaatggtaca     2940

tactatatca cttctcgtga tatgtacaac ccaagagcta ttacaggggg tgatgtagtt     3000

aaactggttt cgtgtcaagc taactatcaa agtattaata gaactgtcat tactacattt     3060

gtagatgaag atgactttaa ttttgaccat gagttgtcta agtggtggaa tgaaactagc     3120

cgtgattttc cagatctcga ccagtttaac tacaccattc ctgtactaaa tattagtaat     3180

gagattgata ggattcaaga agttatacag ggacttaatg actctctaat agaccttgaa     3240

acactatcaa tactcaaaac ttatattaag tggccttggt atgtttggct tgccatattc     3300

tttgccatta ttatctttat ccttatacta ggatgggttt tcttcatgac tggatgttgt     3360

ggttgttgtt gtgggtgctt tggcattatt cctctaatga gtaagtgtgg taagaaatct     3420

tcttactaca ctacttttga taatgatgtg gtaactgaac aatacagacc taaaaagtct     3480

gtttaa                                                                3486


<210>  2
<211>  1161
<212>  PRT
<213>  Avian infectious bronchitis virus

<400>  2

Met Ser Val Leu Leu Leu Leu Gly Leu Thr Leu Leu Ser Ala Leu Cys 
1               5                   10                  15      


Glu Cys Ser Ile Val Gly Asp Asn Tyr Thr Tyr Tyr Tyr His Ser Glu 
            20                  25                  30          


Tyr Arg Pro Pro Asn Gly Trp His Gln Gln Gly Gly Ala Tyr Arg Val 
        35                  40                  45              


Ile Asn Thr Thr Ser Leu Ser Val Ser Thr Cys Ser Leu Gly Gly Val 
    50                  55                  60                  


Pro Pro Leu Leu Thr Val Asn Ala Pro Ser Val Thr Ile Val Ala Pro 
65                  70                  75                  80  


Thr Lys Leu Pro Trp Ser Lys Ala Asn Phe Thr Thr Ala Phe Cys His 
                85                  90                  95      


Phe Gly Asn Phe Ser Val Phe Val Thr His Cys Thr Thr Gly Asn Ser 
            100                 105                 110         


Arg Asn Cys Thr Leu Thr Pro Pro Ile Leu Ala Gly Tyr Ile Val Val 
        115                 120                 125             


Gly Ala Asn Gly Ser Ile Thr Tyr Asn Tyr Thr Leu Arg Ala Ile Gly 
    130                 135                 140                 


Asp Phe Ser His Phe His Ser Phe Gln Cys Val Ser Asn Phe Thr Ser 
145                 150                 155                 160 


Val Tyr Leu Asn Gly Lys Leu Val Phe Thr Ser Asn Leu Ser Leu Gly 
                165                 170                 175     


Val Leu Ser Val Gly Ala Cys Leu Asn Ala Thr Gly Ala Glu Leu Lys 
            180                 185                 190         


Phe Ser Glu Tyr Phe Asn Val Leu Ala Tyr Phe Ile Asn Gly Thr Ala 
        195                 200                 205             


His Asp Val Ile Leu Cys Asp Asp Ser Pro Lys Gly Val Leu Ala Cys 
    210                 215                 220                 


Gln Tyr Asn Thr Gly Asn Phe Thr Asp Gly Phe Tyr Pro Ile Ser Val 
225                 230                 235                 240 


Ser Thr Lys Ala Asp Asp Val Phe Asn Val Tyr Arg Thr Asn Ser Val 
                245                 250                 255     


Leu Ser Tyr Gly Tyr Leu Arg Asn Ile Thr Leu Ser Asn Cys Thr Asp 
            260                 265                 270         


Val Lys Pro Asn Leu Gly Gly Glu Leu Thr Leu Cys Gln Gln Tyr Ser 
        275                 280                 285             


Val Ser Glu Gly Tyr Val Asn Phe Asn Phe Ser Phe Leu Asp Ser Phe 
    290                 295                 300                 


Thr Tyr Val Gly Pro Asp Leu Tyr Asn Phe Asp Tyr Gly Ser Ser His 
305                 310                 315                 320 


Ser Asn Cys His Phe Thr Pro Asn Thr Ile Asn Asn Arg Leu Ser Phe 
                325                 330                 335     


Asn Thr Ile Ser Val Ala Ile Gly Tyr Gly Pro Ile Asp Gly Ala Cys 
            340                 345                 350         


Lys Gln Ile Arg Phe Arg Asn Gly Phe Asn Pro Ser His Thr Cys Cys 
        355                 360                 365             


Tyr Ala Tyr Arg His Pro Ser Leu Ser His Ser Cys His Gly Val Thr 
    370                 375                 380                 


Thr Gly Lys Tyr Ser Asn Ser Tyr Glu Cys Ile Leu Met Val Val Ile 
385                 390                 395                 400 


Ser Arg Asp Tyr Gly Ser Arg Val Gln Thr Gln Thr Glu Val Pro Thr 
                405                 410                 415     


Ser Leu Thr Tyr Phe Tyr Asn Asn Ile Thr Leu Asp Val Cys Val Asp 
            420                 425                 430         


Tyr Asn Ile Tyr Gly Thr Leu Gly Gln Gly Tyr Val Thr Asn Ile Thr 
        435                 440                 445             


Asp Met Ala Leu Ser Gly Gly Tyr Leu Gly Phe Gly Gly Leu Val Ile 
    450                 455                 460                 


Leu Asp Ser Ser Gly Ala Ile Asp Ile Phe Leu Val Glu Ser Glu Asn 
465                 470                 475                 480 


Gly Pro Asn Tyr Tyr Lys Ile Asn Pro Cys Asn Asp Val Asn Gln Gln 
                485                 490                 495     


Tyr Val Val Ser Gly Gly Ser Ile Val Gly Leu Leu Thr Ser Asn Asn 
            500                 505                 510         


Leu Thr Gly Ser Glu Leu Leu Glu Asp Gln Phe Tyr Val Lys Phe Thr 
        515                 520                 525             


Asn Asn Val His Arg Arg Arg Arg Ser Ile Ser Glu Asn Val Thr Ser 
    530                 535                 540                 


Cys Pro Tyr Pro Thr Tyr Gly Lys Phe Cys Ile Lys Pro Asp Gly Ser 
545                 550                 555                 560 


Val Ser Thr Ile Val Pro Glu Glu Val Lys Asp Tyr Thr Ser Leu Leu 
                565                 570                 575     


Leu Asn Arg Thr Asp Tyr Val Leu Ile Pro Asp Ser Phe Asn Leu Thr 
            580                 585                 590         


Val Thr Asp Glu Phe Ile Gln Thr Gln Met Gln Gln Val Gln Ile Asn 
        595                 600                 605             


Cys Ile Gln Tyr Val Cys Gly Asn Ser Ile Gln Cys Lys Gln Leu Phe 
    610                 615                 620                 


Arg Gln Tyr Gly Pro Val Cys Asp Ser Ile Leu Ser Ile Val Asn Gly 
625                 630                 635                 640 


Ile Val Gln Gln Asp Asn Ala Glu Ile Leu Gln Phe Tyr Ser Ser Thr 
                645                 650                 655     


Lys Pro Lys Gly Phe Asp Glu Ser Ser Phe Ser Asn Phe Thr Ala Gly 
            660                 665                 670         


Glu Phe Asn Ile Ser Leu Val Leu Pro Lys Asn Gly Arg Pro Thr Gly 
        675                 680                 685             


Arg Ser Phe Ile Glu Asp Leu Leu Phe Asp Lys Val Glu Ser Leu Gly 
    690                 695                 700                 


Leu Pro Gly Asp Ser Thr Tyr Gln Lys Cys Thr Ser Gly Pro Leu Gly 
705                 710                 715                 720 


Phe Val Lys Asp Leu Val Cys Ala Gln Lys Tyr Asn Gly Leu Leu Val 
                725                 730                 735     


Leu Pro Pro Ile Ile Thr Ala Glu Met Gln Ala Met Tyr Thr Ser Ser 
            740                 745                 750         


Leu Val Ala Ser Met Ala Phe Gly Gly Ile Thr Ala Ala Gly Ala Ile 
        755                 760                 765             


Pro Phe Ala Thr Gln Leu Gln Ala Arg Ile Asn His Leu Gly Ile Thr 
    770                 775                 780                 


Gln Thr Val Leu Gln Lys Asn Gln Glu Lys Ile Ala Ala Ser Phe Asn 
785                 790                 795                 800 


Lys Ala Met Val His Met Gln Asp Gly Phe Lys Ala Thr Ser Leu Ala 
                805                 810                 815     


Leu Gln Gln Val Gln Asp Val Val Asn Glu His Gly Ala Ile Leu Gln 
            820                 825                 830         


Gln Thr Met His Ser Leu Asn Lys Asn Phe Gly Ala Ile Thr His Val 
        835                 840                 845             


Ile Gln Asp Ile Tyr Lys Gln Leu Asp Ala Leu Glu Ala Asn Ala Gln 
    850                 855                 860                 


Val Asp Arg Leu Ile Asn Gly Arg Leu Ser Ser Leu Ser Val Leu Ala 
865                 870                 875                 880 


Ser Ser Lys Gln Leu Glu Tyr Thr Lys Val Ser His Gln Arg Glu Leu 
                885                 890                 895     


Ala Lys Glu Lys Ile Asn Glu Cys Val Lys Ser Gln Ser Ser Arg Tyr 
            900                 905                 910         


Gly Phe Cys Gly Asp Gly Met His Ile Met Ser Ile Pro Gln Ser Ala 
        915                 920                 925             


Pro Asn Gly Ile Val Phe Leu His Phe Thr Tyr Thr Pro Val Ser Phe 
    930                 935                 940                 


Gln Asn Val Thr Ala Val Val Gly Phe Cys Val Lys Pro Val Asn Gly 
945                 950                 955                 960 


Ser Glu Tyr Gly Leu Val Pro Val Val Gly Arg Gly Ile Phe Ile Glu 
                965                 970                 975     


Val Asn Gly Thr Tyr Tyr Ile Thr Ser Arg Asp Met Tyr Asn Pro Arg 
            980                 985                 990         


Ala Ile Thr Gly Gly Asp Val Val  Lys Leu Val Ser Cys  Gln Ala Asn 
        995                 1000                 1005             


Tyr Gln  Ser Ile Asn Arg Thr  Val Ile Thr Thr Phe  Val Asp Glu 
    1010                 1015                 1020             


Asp Asp  Phe Asn Phe Asp His  Glu Leu Ser Lys Trp  Trp Asn Glu 
    1025                 1030                 1035             


Thr Ser  Arg Asp Phe Pro Asp  Leu Asp Gln Phe Asn  Tyr Thr Ile 
    1040                 1045                 1050             


Pro Val  Leu Asn Ile Ser Asn  Glu Ile Asp Arg Ile  Gln Glu Val 
    1055                 1060                 1065             


Ile Gln  Gly Leu Asn Asp Ser  Leu Ile Asp Leu Glu  Thr Leu Ser 
    1070                 1075                 1080             


Ile Leu  Lys Thr Tyr Ile Lys  Trp Pro Trp Tyr Val  Trp Leu Ala 
    1085                 1090                 1095             


Ile Phe  Phe Ala Ile Ile Ile  Phe Ile Leu Ile Leu  Gly Trp Val 
    1100                 1105                 1110             


Phe Phe  Met Thr Gly Cys Cys  Gly Cys Cys Cys Gly  Cys Phe Gly 
    1115                 1120                 1125             


Ile Ile  Pro Leu Met Ser Lys  Cys Gly Lys Lys Ser  Ser Tyr Tyr 
    1130                 1135                 1140             


Thr Thr  Phe Asp Asn Asp Val  Val Thr Glu Gln Tyr  Arg Pro Lys 
    1145                 1150                 1155             


Lys Ser  Val 
    1160     


<210>  3
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer (Sequenz 5-3) fr IB80 Real-Time RT-PCR

<400>  3
agtgtagtat agtaggtgac aat                                               23


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer (Sequenz 5-3) fr IB80 Real-Time RT-PCR

<400>  4
acatcatgtg ctgtaccatt                                                   20


<210>  5
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sonde (Sequenz 5-3) fr IB80 Real-Time RT-PCR


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  BHQ1

<400>  5
nccacctatt ttagcaggtt atattgtagt tggtn                                  35


