                         SEQUENCE LISTING

<110>  University of Miami
 
<120>  TRI-NUCLEOTIDE ROLLING CIRCLE AMPLIFICATION

<130>  32286/51002A PC

<150>  US 62/384,470
<151>  2016-09-07

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  Zika virus target sequence

<400>  1
tgttggtatg gaatggagat aagg                                              24


<210>  2
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Zika virus synthetic DNA target

<400>  2
taaagatggc tgttggtatg gaatggagat aaggcccagg aaag                        44


<210>  3
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Zika virus synthetic RNA target

<400>  3
tttcctgggc cttatctcc                                                    19


<210>  4
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Padlock A for Zika virus target

<400>  4
tccataccaa catttttatc ttaactcacc aacaccattt tttctaatct caaccttact       60

acactctttt ttccttatct ccat                                              84


<210>  5
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  5' end antisense to target

<400>  5
tccataccaa ca                                                           12


<210>  6
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  3' end antisense to target

<400>  6
ccttatctcc at                                                           12


<210>  7
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Start Primer

<400>  7
tggtgttggt gagttaag                                                     18


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Forward primer to generate Zika target DNA by PCR

<400>  8
gcttgaaatt cggtttgagg                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Reverse primer to generate Zika target DNA by PCR

<400>  9
ctttcctggg ccttatctcc                                                   20


<210>  10
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  HPV DNA target  sequence

<400>  10
gaggaatatg atttacagtt tatttttga                                         29


<210>  11
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Padlock for HPV target- Full Sequence

<400>  11
catattcctc tttttatctt aactcaccaa caccattttt tctaatctca accttactac       60

actctttttt tcaaaaataa actctaaat                                         89


<210>  12
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  5' end antisense to target

<400>  12
catattcctc                                                              10


<210>  13
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  3' end antisense to target

<400>  13
tcaaaaataa actctaaat                                                    19


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  HPV forward universal primer to 

generate target DNA by PCR

<400>  14
tttgttactg tggtagatac tac                                               23


<210>  15
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  HPV reverse universal primer 

GP6+ to generate target DNA by PCR

<400>  15
gaaaaataaa ctgtaaatca tattc                                             25


<210>  16
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Norovirus RNA target sequence

<400>  16
gaguugguau gaauaugaau gagga                                             25


<210>  17
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Padlock for Norovirus target- Full Sequence

<400>  17
tcataccaac tctttttatc ttaactcacc aacaccatct caaccttact acactctttt       60

ttcctcattc atat                                                         74


<210>  18
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  5' end antisense to target

<400>  18
tcataccaac tc                                                           12


<210>  19
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  3' end antisense to target

<400>  19
cctcattcat at                                                           12


<210>  20
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  Biotin

<400>  20
ctcaacctta ctacactc                                                     18


<210>  21
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  Msecutprimer

<400>  21
ctcaacctta ctacactc                                                     18


<210>  22
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  Padlock probe

<400>  22
tccataccaa catttttatc ttaactcacc aacaccatct caaccttact acactctttt       60

ttccttatct ccat                                                         74


<210>  23
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  Padlock Probe

<400>  23
tccataccaa catttttatc ttaactcacc aacaccatct caaccttact acactctttt       60

ttccctatct ccat                                                         74


<210>  24
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  Padlock probe

<400>  24
tccataccat catttttatc ttaactcacc aacaccatct caaccttact acactctttt       60

ttccctatct ccat                                                         74


