                         SEQUENCE LISTING

<110>  The General Hospital Corporation
       Poznansky, Mark C.
       Gelfand, Jeffrey A.
 
<120>  EX VIVO ANTIGEN-PRESENTING CELLS OR ACTIVATED CD-POSITIVE T CELLS
        FOR TREATMENT OF CANCER

<130>  1417-17WO

<150>  US 62/385,898
<151>  2016-09-09

<150>  US 62/385,880
<151>  2016-09-09

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  915
<212>  PRT
<213>  Artificial

<220>
<223>  fusion protein

<400>  1

Gly Ser Ser Gln Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Thr 
1               5                   10                  15      


Pro Ser Gln Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly Asp Ser Val 
            20                  25                  30          


Ser Ser Asn Ser Ala Thr Trp Asn Trp Ile Arg Gln Ser Pro Ser Arg 
        35                  40                  45              


Gly Leu Glu Trp Leu Gly Arg Thr Tyr Tyr Arg Ser Lys Trp Tyr Asn 
    50                  55                  60                  


Asp Tyr Ala Val Ser Val Lys Ser Arg Met Ser Ile Asn Pro Asp Thr 
65                  70                  75                  80  


Ser Lys Asn Gln Phe Ser Leu Gln Leu Asn Ser Val Thr Pro Glu Asp 
                85                  90                  95      


Thr Ala Val Tyr Tyr Cys Ala Arg Gly Met Met Thr Tyr Tyr Tyr Gly 
            100                 105                 110         


Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Ile 
        115                 120                 125             


Leu Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gln Pro Val Leu Thr Gln Ser Ser Ser Leu Ser Ala Ser Pro 
145                 150                 155                 160 


Gly Ala Ser Ala Ser Leu Thr Cys Thr Leu Arg Ser Gly Ile Asn Val 
                165                 170                 175     


Gly Pro Tyr Arg Ile Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Pro Pro 
            180                 185                 190         


Gln Tyr Leu Leu Asn Tyr Lys Ser Asp Ser Asp Lys Gln Gln Gly Ser 
        195                 200                 205             


Gly Val Pro Ser Arg Phe Ser Gly Ser Lys Asp Ala Ser Ala Asn Ala 
    210                 215                 220                 


Gly Val Leu Leu Ile Ser Gly Leu Arg Ser Glu Asp Glu Ala Asp Tyr 
225                 230                 235                 240 


Tyr Cys Met Ile Trp His Ser Ser Ala Ala Val Phe Gly Gly Gly Thr 
                245                 250                 255     


Gln Leu Thr Val Leu Ser Gly Ile Leu Glu Gln Gln Gly Gly Gly Gly 
            260                 265                 270         


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ala Ala Met 
        275                 280                 285             


Arg Ser Met Ala Arg Ala Val Gly Ile Asp Leu Gly Thr Thr Asn Ser 
    290                 295                 300                 


Val Val Ser Val Leu Glu Gly Gly Asp Pro Val Val Val Ala Asn Ser 
305                 310                 315                 320 


Glu Gly Ser Arg Thr Thr Pro Ser Ile Val Ala Phe Ala Arg Asn Gly 
                325                 330                 335     


Glu Val Leu Val Gly Gln Pro Ala Lys Asn Gln Ala Val Thr Asn Val 
            340                 345                 350         


Asp Arg Thr Val Arg Ser Val Lys Arg His Met Gly Ser Asp Trp Ser 
        355                 360                 365             


Ile Glu Ile Asp Gly Lys Lys Tyr Thr Ala Pro Glu Ile Ser Ala Arg 
    370                 375                 380                 


Ile Leu Met Lys Leu Lys Arg Asp Ala Glu Ala Tyr Leu Gly Glu Asp 
385                 390                 395                 400 


Ile Thr Asp Ala Val Ile Thr Thr Pro Ala Tyr Phe Asn Asp Ala Gln 
                405                 410                 415     


Arg Gln Ala Thr Lys Asp Ala Gly Gln Ile Ala Gly Leu Asn Val Leu 
            420                 425                 430         


Arg Ile Val Asn Glu Pro Thr Ala Ala Ala Leu Ala Tyr Gly Leu Asp 
        435                 440                 445             


Lys Gly Glu Lys Glu Gln Arg Ile Leu Val Phe Asp Leu Gly Gly Gly 
    450                 455                 460                 


Thr Phe Asp Val Ser Leu Leu Glu Ile Gly Glu Gly Val Val Glu Val 
465                 470                 475                 480 


Arg Ala Thr Ser Gly Asp Asn His Leu Gly Gly Asp Asp Trp Asp Gln 
                485                 490                 495     


Arg Val Val Asp Trp Leu Val Asp Lys Phe Lys Gly Thr Ser Gly Ile 
            500                 505                 510         


Asp Leu Thr Lys Asp Lys Met Ala Met Gln Arg Leu Arg Glu Ala Ala 
        515                 520                 525             


Glu Lys Ala Lys Ile Glu Leu Ser Ser Ser Gln Ser Thr Ser Ile Asn 
    530                 535                 540                 


Leu Pro Tyr Ile Thr Val Asp Ala Asp Lys Asn Pro Leu Phe Leu Asp 
545                 550                 555                 560 


Glu Gln Leu Thr Arg Ala Glu Phe Gln Arg Ile Thr Gln Asp Leu Leu 
                565                 570                 575     


Asp Arg Thr Arg Lys Pro Phe Gln Ser Val Ile Ala Asp Thr Gly Ile 
            580                 585                 590         


Ser Val Ser Glu Ile Asp His Val Val Leu Val Gly Gly Ser Thr Arg 
        595                 600                 605             


Met Pro Ala Val Thr Asp Leu Val Lys Glu Leu Thr Gly Gly Lys Glu 
    610                 615                 620                 


Pro Asn Lys Gly Val Asn Pro Asp Glu Val Val Ala Val Gly Ala Ala 
625                 630                 635                 640 


Leu Gln Ala Gly Val Leu Lys Gly Glu Val Lys Asp Val Leu Leu Leu 
                645                 650                 655     


Asp Val Thr Pro Leu Ser Leu Gly Ile Glu Thr Lys Gly Gly Phe Met 
            660                 665                 670         


Thr Arg Leu Ile Glu Arg Asn Thr Thr Ile Pro Thr Lys Arg Ser Glu 
        675                 680                 685             


Thr Phe Thr Thr Ala Asp Asp Asn Gln Pro Ser Val Gln Ile Gln Val 
    690                 695                 700                 


Tyr Gln Gly Glu Arg Glu Ile Ala Ala His Asn Lys Leu Leu Gly Ser 
705                 710                 715                 720 


Phe Glu Leu Thr Gly Ile Pro Pro Ala Pro Arg Gly Ile Pro Gln Ile 
                725                 730                 735     


Glu Val Thr Phe Asp Ile Asp Ala Asn Gly Ile Val His Val Thr Ala 
            740                 745                 750         


Lys Asp Lys Gly Thr Gly Lys Glu Asn Thr Ile Arg Ile Gln Glu Gly 
        755                 760                 765             


Ser Gly Leu Ser Lys Glu Asp Ile Asp Arg Met Ile Lys Asp Ala Glu 
    770                 775                 780                 


Ala His Ala Glu Glu Asp Arg Lys Arg Arg Glu Glu Ala Asp Val Arg 
785                 790                 795                 800 


Asn Gln Ala Glu Thr Leu Val Tyr Gln Thr Glu Lys Phe Val Lys Glu 
                805                 810                 815     


Gln Arg Glu Ala Glu Gly Gly Ser Lys Val Pro Glu Asp Thr Leu Asn 
            820                 825                 830         


Lys Val Asp Ala Ala Val Ala Glu Ala Lys Ala Ala Leu Gly Gly Ser 
        835                 840                 845             


Asp Ile Ser Ala Ile Lys Ser Ala Met Glu Lys Leu Gly Gln Glu Ser 
    850                 855                 860                 


Gln Ala Leu Gly Gln Ala Ile Tyr Glu Ala Ala Gln Ala Ala Ser Gln 
865                 870                 875                 880 


Ala Thr Gly Ala Ala His Pro Gly Gly Glu Pro Gly Gly Ala His Pro 
                885                 890                 895     


Gly Ser Ala Asp Asp Val Val Asp Ala Glu Val Val Asp Asp Gly Arg 
            900                 905                 910         


Glu Ala Lys 
        915 


<210>  2
<211>  898
<212>  PRT
<213>  Artificial

<220>
<223>  fusion protein

<400>  2

Gln Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Thr Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly Asp Ser Val Ser Ser Asn 
            20                  25                  30          


Ser Ala Thr Trp Asn Trp Ile Arg Gln Ser Pro Ser Arg Gly Leu Glu 
        35                  40                  45              


Trp Leu Gly Arg Thr Tyr Tyr Arg Ser Lys Trp Tyr Asn Asp Tyr Ala 
    50                  55                  60                  


Val Ser Val Lys Ser Arg Met Ser Ile Asn Pro Asp Thr Ser Lys Asn 
65                  70                  75                  80  


Gln Phe Ser Leu Gln Leu Asn Ser Val Thr Pro Glu Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Gly Met Met Thr Tyr Tyr Tyr Gly Met Asp Val 
            100                 105                 110         


Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Ile Leu Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln 
    130                 135                 140                 


Pro Val Leu Thr Gln Ser Ser Ser Leu Ser Ala Ser Pro Gly Ala Ser 
145                 150                 155                 160 


Ala Ser Leu Thr Cys Thr Leu Arg Ser Gly Ile Asn Val Gly Pro Tyr 
                165                 170                 175     


Arg Ile Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Pro Pro Gln Tyr Leu 
            180                 185                 190         


Leu Asn Tyr Lys Ser Asp Ser Asp Lys Gln Gln Gly Ser Gly Val Pro 
        195                 200                 205             


Ser Arg Phe Ser Gly Ser Lys Asp Ala Ser Ala Asn Ala Gly Val Leu 
    210                 215                 220                 


Leu Ile Ser Gly Leu Arg Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Met 
225                 230                 235                 240 


Ile Trp His Ser Ser Ala Ala Val Phe Gly Gly Gly Thr Gln Leu Thr 
                245                 250                 255     


Val Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            260                 265                 270         


Ser Met Ala Arg Ala Val Gly Ile Asp Leu Gly Thr Thr Asn Ser Val 
        275                 280                 285             


Val Ser Val Leu Glu Gly Gly Asp Pro Val Val Val Ala Asn Ser Glu 
    290                 295                 300                 


Gly Ser Arg Thr Thr Pro Ser Ile Val Ala Phe Ala Arg Asn Gly Glu 
305                 310                 315                 320 


Val Leu Val Gly Gln Pro Ala Lys Asn Gln Ala Val Thr Asn Val Asp 
                325                 330                 335     


Arg Thr Val Arg Ser Val Lys Arg His Met Gly Ser Asp Trp Ser Ile 
            340                 345                 350         


Glu Ile Asp Gly Lys Lys Tyr Thr Ala Pro Glu Ile Ser Ala Arg Ile 
        355                 360                 365             


Leu Met Lys Leu Lys Arg Asp Ala Glu Ala Tyr Leu Gly Glu Asp Ile 
    370                 375                 380                 


Thr Asp Ala Val Ile Thr Thr Pro Ala Tyr Phe Asn Asp Ala Gln Arg 
385                 390                 395                 400 


Gln Ala Thr Lys Asp Ala Gly Gln Ile Ala Gly Leu Asn Val Leu Arg 
                405                 410                 415     


Ile Val Asn Glu Pro Thr Ala Ala Ala Leu Ala Tyr Gly Leu Asp Lys 
            420                 425                 430         


Gly Glu Lys Glu Gln Arg Ile Leu Val Phe Asp Leu Gly Gly Gly Thr 
        435                 440                 445             


Phe Asp Val Ser Leu Leu Glu Ile Gly Glu Gly Val Val Glu Val Arg 
    450                 455                 460                 


Ala Thr Ser Gly Asp Asn His Leu Gly Gly Asp Asp Trp Asp Gln Arg 
465                 470                 475                 480 


Val Val Asp Trp Leu Val Asp Lys Phe Lys Gly Thr Ser Gly Ile Asp 
                485                 490                 495     


Leu Thr Lys Asp Lys Met Ala Met Gln Arg Leu Arg Glu Ala Ala Glu 
            500                 505                 510         


Lys Ala Lys Ile Glu Leu Ser Ser Ser Gln Ser Thr Ser Ile Asn Leu 
        515                 520                 525             


Pro Tyr Ile Thr Val Asp Ala Asp Lys Asn Pro Leu Phe Leu Asp Glu 
    530                 535                 540                 


Gln Leu Thr Arg Ala Glu Phe Gln Arg Ile Thr Gln Asp Leu Leu Asp 
545                 550                 555                 560 


Arg Thr Arg Lys Pro Phe Gln Ser Val Ile Ala Asp Thr Gly Ile Ser 
                565                 570                 575     


Val Ser Glu Ile Asp His Val Val Leu Val Gly Gly Ser Thr Arg Met 
            580                 585                 590         


Pro Ala Val Thr Asp Leu Val Lys Glu Leu Thr Gly Gly Lys Glu Pro 
        595                 600                 605             


Asn Lys Gly Val Asn Pro Asp Glu Val Val Ala Val Gly Ala Ala Leu 
    610                 615                 620                 


Gln Ala Gly Val Leu Lys Gly Glu Val Lys Asp Val Leu Leu Leu Asp 
625                 630                 635                 640 


Val Thr Pro Leu Ser Leu Gly Ile Glu Thr Lys Gly Gly Phe Met Thr 
                645                 650                 655     


Arg Leu Ile Glu Arg Asn Thr Thr Ile Pro Thr Lys Arg Ser Glu Thr 
            660                 665                 670         


Phe Thr Thr Ala Asp Asp Asn Gln Pro Ser Val Gln Ile Gln Val Tyr 
        675                 680                 685             


Gln Gly Glu Arg Glu Ile Ala Ala His Asn Lys Leu Leu Gly Ser Phe 
    690                 695                 700                 


Glu Leu Thr Gly Ile Pro Pro Ala Pro Arg Gly Ile Pro Gln Ile Glu 
705                 710                 715                 720 


Val Thr Phe Asp Ile Asp Ala Asn Gly Ile Val His Val Thr Ala Lys 
                725                 730                 735     


Asp Lys Gly Thr Gly Lys Glu Asn Thr Ile Arg Ile Gln Glu Gly Ser 
            740                 745                 750         


Gly Leu Ser Lys Glu Asp Ile Asp Arg Met Ile Lys Asp Ala Glu Ala 
        755                 760                 765             


His Ala Glu Glu Asp Arg Lys Arg Arg Glu Glu Ala Asp Val Arg Asn 
    770                 775                 780                 


Gln Ala Glu Thr Leu Val Tyr Gln Thr Glu Lys Phe Val Lys Glu Gln 
785                 790                 795                 800 


Arg Glu Ala Glu Gly Gly Ser Lys Val Pro Glu Asp Thr Leu Asn Lys 
                805                 810                 815     


Val Asp Ala Ala Val Ala Glu Ala Lys Ala Ala Leu Gly Gly Ser Asp 
            820                 825                 830         


Ile Ser Ala Ile Lys Ser Ala Met Glu Lys Leu Gly Gln Glu Ser Gln 
        835                 840                 845             


Ala Leu Gly Gln Ala Ile Tyr Glu Ala Ala Gln Ala Ala Ser Gln Ala 
    850                 855                 860                 


Thr Gly Ala Ala His Pro Gly Gly Glu Pro Gly Gly Ala His Pro Gly 
865                 870                 875                 880 


Ser Ala Asp Asp Val Val Asp Ala Glu Val Val Asp Asp Gly Arg Glu 
                885                 890                 895     


Ala Lys 
        


