                         SEQUENCE LISTING

<110>  bluebird bio, Inc.
       Mann, Jasdeep
       Gay, Joel
       Jarjour, Jordan
       Zhang, Joy
 
<120>  PD-1 HOMING ENDONUCLEASE VARIANTS, COMPOSITIONS, AND METHODS OF 
       USE

<130>  BLBD-076/02WO

<150>  US 62/414,279
<151>  2016-10-28

<150>  US 62/385,079
<151>  2016-09-08

<160>  123   

<170>  PatentIn version 3.5

<210>  1
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi subsp. americana (mitochondrion)

<400>  1

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Met Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Cys Val Met Glu Asn Lys Glu His Leu Lys Ile Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Ile Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  2
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi subsp. americana (mitochondrion)

<400>  2

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  3
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi subsp. americana (mitochondrion)


<220>
<221>  MOD_RES
<222>  (1)..(3)
<223>  Any amino acid or absent

<400>  3

Xaa Xaa Xaa Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  4
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi subsp. americana (mitochondrion)


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  4

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  5
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi subsp. americana (mitochondrion)


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  5

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  6
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant PD-1.ITSM.ex5_RD1_CV3-08


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  6

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Gln Leu Glu Ile Arg Asn Val 
            20                  25                  30          


Asn Pro Asn Ile Pro Arg Tyr Lys Thr Arg Leu Arg Phe Glu Ile Asp 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Tyr Asn Gln Gly Asp Ser Tyr Val Lys Leu Arg 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly His Phe Gly Val Ile Leu Ala Lys Arg Arg Pro Ala Ser 
            180                 185                 190         


Pro Val Gln Val Arg Leu Arg Phe Ala Ile Gly Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Arg Ile 
    210                 215                 220                 


Arg Glu Lys Asn Ile Ser Glu Lys Ser Trp Leu Glu Phe Glu Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  7
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant PD-1.ITSM.ex5_RD2_73


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  7

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Gln Leu Glu Ile Arg Asn Val 
            20                  25                  30          


Asn Pro Asn Ile Pro Arg Tyr Arg Thr Arg Leu Arg Phe Glu Ile Asp 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asp Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Tyr Asn Arg Gly Asp Ser Tyr Val Lys Leu Arg 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly His Phe Gly Val Ile Leu Ala Lys Arg Arg Pro Ala Ser 
            180                 185                 190         


Pro Val Gln Val Arg Leu Arg Phe Ala Ile Gly Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Arg Thr 
    210                 215                 220                 


Arg Glu Lys Asn Ile Ser Glu Lys Ser Trp Leu Glu Phe Glu Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  8
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant PD-1.ITSM.ex5_RD3_03


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  8

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Gln Leu Glu Ile Arg Asn Val 
            20                  25                  30          


Asn Pro Asn Ile Pro Arg Tyr Arg Thr Arg Leu Arg Phe Glu Ile Asp 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asp Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Tyr Asn Gln Gly Asp Ser Tyr Val Lys Leu Arg 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly His Phe Gly Val Ile Leu Ala Lys Arg Arg Pro Ala Ser 
            180                 185                 190         


Pro Val Gln Val Arg Leu Arg Phe Ala Ile Gly Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Arg Thr 
    210                 215                 220                 


Arg Glu Lys Asn Ile Ser Glu Lys Ser Trp Leu Glu Phe Glu Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  9
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant PD-1.ITSM.ex5_RD4_CV23


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  9

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Gln Leu Glu Ile Arg Asn Val 
            20                  25                  30          


Asn Pro Asn Ile Pro Arg Tyr Arg Thr Arg Leu Arg Phe Glu Ile Asp 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asp Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Tyr Asn Gln Gly Asp Ser Tyr Val Lys Leu Arg 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly His Phe Gly Val Ile Leu Ala Lys Arg Arg Pro Ala Ser 
            180                 185                 190         


Pro Val Gln Val Arg Leu Arg Phe Ala Ile Gly Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Arg Ile 
    210                 215                 220                 


Arg Glu Lys Asn Lys Ser Glu Lys Ser Trp Leu Glu Phe Glu Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  10
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant PD-1.ITSM.ex5_RD5_CV23MK


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  10

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Gln Leu Glu Ile Arg Asn Val 
            20                  25                  30          


Asn Pro Asn Ile Pro Arg Tyr Arg Thr Arg Leu Arg Phe Glu Ile Asp 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asp Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Tyr Asn Gln Gly Asp Ser Tyr Val Lys Leu Arg 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly His Phe Gly Val Ile Leu Ala Lys Arg Arg Pro Ala Ser 
            180                 185                 190         


Pro Val Gln Val Arg Leu Arg Phe Met Ile Gly Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Arg Ile 
    210                 215                 220                 


Arg Glu Lys Asn Lys Ser Glu Lys Ser Trp Leu Glu Phe Glu Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  11
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant PD-1.ile3.exon1_RD1_B1


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  11

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Gly Leu Ser Ile Leu Asn Arg 
            20                  25                  30          


Asn Arg Gly Thr Ala Arg Tyr His Thr Arg Leu Ser Phe Ala Ile Val 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Ile Ile Thr Asn Asp Gly Asp Arg Tyr Val Arg Leu Arg 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Val Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  12
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant PD-1.ile3.exon1_RD2_B1H8


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  12

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Gly Leu Ser Ile Leu Asn Arg 
            20                  25                  30          


Asn Arg Gly Thr Ala Arg Tyr His Thr Arg Leu Ser Phe Thr Ile Val 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Ile Ile Thr Asn Asp Gly Asp Arg Tyr Val Arg Leu Cys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Val Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Ala Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Gly Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  13
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant PD-1.IgV.exon2_RD1_G5


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  13

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Gln Leu Tyr Ile Ser Asn Val 
            20                  25                  30          


Asn Asn Asn Arg Ser Arg Tyr Arg Ala Arg Leu Arg Phe Ala Ile Glu 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Asn Asn Ile Gly Asp Thr Ser Val Arg Leu Ser 
65                  70                  75                  80  


Val Gly Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Asn Phe Tyr Val His Leu Lys Lys Ser Gly Arg Thr Thr 
            180                 185                 190         


Arg Val Tyr Val Gln Leu Arg Phe Ser Ile Ala Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Asn Glu Trp Asn Ala Ser Glu Arg Ser Ala Leu Glu Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  14
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant PD-1.IgV.exon2_RD1_PS3


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  14

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Cys Phe Leu Leu His Ile Arg Asn Leu 
            20                  25                  30          


Asn Arg Thr Ser Thr Lys Tyr Arg Thr Arg Leu Ser Phe Glu Ile Glu 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Ile Ile Asn Asn Ile Gly Asn Arg Arg Val Arg Leu Ser 
65                  70                  75                  80  


Val Arg Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Leu Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Asn Phe Tyr Val His Leu Lys Lys Ser Gly Arg Thr Thr 
            180                 185                 190         


Arg Val Tyr Val Gln Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Thr Glu Ser Asn Pro Ser Glu Arg Ser Asp Leu Glu Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  15
<211>  870
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized megaTAL PD-1.ITSM.ex5_RD1_CV3-08

<400>  15

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
225                 230                 235                 240 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                245                 250                 255     


Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            260                 265                 270         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        275                 280                 285             


Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr 
    290                 295                 300                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
305                 310                 315                 320 


Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu 
                325                 330                 335     


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
            340                 345                 350         


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln 
        355                 360                 365             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
    370                 375                 380                 


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
385                 390                 395                 400 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                405                 410                 415     


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
            420                 425                 430         


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
        435                 440                 445             


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
    450                 455                 460                 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
465                 470                 475                 480 


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
                485                 490                 495     


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala Gln 
            500                 505                 510         


Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu 
        515                 520                 525             


Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val Lys 
    530                 535                 540                 


Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg Arg 
545                 550                 555                 560 


Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Ser Arg Arg 
                565                 570                 575     


Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu Gly 
            580                 585                 590         


Ser Phe Gln Leu Glu Ile Arg Asn Val Asn Pro Asn Ile Pro Arg Tyr 
        595                 600                 605             


Lys Thr Arg Leu Arg Phe Glu Ile Asp Leu His Asn Lys Asp Lys Ser 
    610                 615                 620                 


Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Lys Ile Tyr Asn 
625                 630                 635                 640 


Gln Gly Asp Ser Tyr Val Lys Leu Arg Val Thr Arg Phe Glu Asp Leu 
                645                 650                 655     


Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys 
            660                 665                 670         


Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Met Glu Asn 
        675                 680                 685             


Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile Lys 
    690                 695                 700                 


Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu Lys Lys Ala Phe Pro 
705                 710                 715                 720 


Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro Asn 
                725                 730                 735     


Leu Lys Trp Leu Ala Gly Phe Thr Ser Gly Glu Gly His Phe Gly Val 
            740                 745                 750         


Ile Leu Ala Lys Arg Arg Pro Ala Ser Pro Val Gln Val Arg Leu Arg 
        755                 760                 765             


Phe Ala Ile Gly Gln His Ile Arg Asp Lys Asn Leu Met Asn Ser Leu 
    770                 775                 780                 


Ile Thr Tyr Leu Gly Cys Gly Arg Ile Arg Glu Lys Asn Ile Ser Glu 
785                 790                 795                 800 


Lys Ser Trp Leu Glu Phe Glu Val Thr Lys Phe Ser Asp Ile Asn Asp 
                805                 810                 815     


Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys Leu 
            820                 825                 830         


Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu Lys 
        835                 840                 845             


Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu 
    850                 855                 860                 


Asn Met Asn Lys Gly Arg 
865                 870 


<210>  16
<211>  870
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized megaTAL PD-1.ITSM.ex5_RD2_73

<400>  16

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
225                 230                 235                 240 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                245                 250                 255     


Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            260                 265                 270         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        275                 280                 285             


Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr 
    290                 295                 300                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
305                 310                 315                 320 


Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu 
                325                 330                 335     


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
            340                 345                 350         


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln 
        355                 360                 365             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
    370                 375                 380                 


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
385                 390                 395                 400 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                405                 410                 415     


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
            420                 425                 430         


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
        435                 440                 445             


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
    450                 455                 460                 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
465                 470                 475                 480 


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
                485                 490                 495     


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala Gln 
            500                 505                 510         


Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu 
        515                 520                 525             


Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val Lys 
    530                 535                 540                 


Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg Arg 
545                 550                 555                 560 


Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Ser Arg Arg 
                565                 570                 575     


Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu Gly 
            580                 585                 590         


Ser Phe Gln Leu Glu Ile Arg Asn Val Asn Pro Asn Ile Pro Arg Tyr 
        595                 600                 605             


Arg Thr Arg Leu Arg Phe Glu Ile Asp Leu His Asn Lys Asp Lys Ser 
    610                 615                 620                 


Ile Leu Glu Asp Ile Gln Ser Thr Trp Lys Val Gly Lys Ile Tyr Asn 
625                 630                 635                 640 


Arg Gly Asp Ser Tyr Val Lys Leu Arg Val Thr Arg Phe Glu Asp Leu 
                645                 650                 655     


Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys 
            660                 665                 670         


Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Met Glu Asn 
        675                 680                 685             


Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile Lys 
    690                 695                 700                 


Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu Lys Lys Ala Phe Pro 
705                 710                 715                 720 


Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro Asn 
                725                 730                 735     


Leu Lys Trp Leu Ala Gly Phe Thr Ser Gly Glu Gly His Phe Gly Val 
            740                 745                 750         


Ile Leu Ala Lys Arg Arg Pro Ala Ser Pro Val Gln Val Arg Leu Arg 
        755                 760                 765             


Phe Ala Ile Gly Gln His Ile Arg Asp Lys Asn Leu Met Asn Ser Leu 
    770                 775                 780                 


Ile Thr Tyr Leu Gly Cys Gly Arg Thr Arg Glu Lys Asn Ile Ser Glu 
785                 790                 795                 800 


Lys Ser Trp Leu Glu Phe Glu Val Thr Lys Phe Ser Asp Ile Asn Asp 
                805                 810                 815     


Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys Leu 
            820                 825                 830         


Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu Lys 
        835                 840                 845             


Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu 
    850                 855                 860                 


Asn Met Asn Lys Gly Arg 
865                 870 


<210>  17
<211>  870
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized megaTAL PD-1.ITSM.ex5_RD3_03

<400>  17

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
225                 230                 235                 240 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                245                 250                 255     


Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            260                 265                 270         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        275                 280                 285             


Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr 
    290                 295                 300                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
305                 310                 315                 320 


Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu 
                325                 330                 335     


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
            340                 345                 350         


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln 
        355                 360                 365             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
    370                 375                 380                 


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
385                 390                 395                 400 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                405                 410                 415     


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
            420                 425                 430         


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
        435                 440                 445             


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
    450                 455                 460                 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
465                 470                 475                 480 


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
                485                 490                 495     


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala Gln 
            500                 505                 510         


Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu 
        515                 520                 525             


Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val Lys 
    530                 535                 540                 


Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg Arg 
545                 550                 555                 560 


Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Ser Arg Arg 
                565                 570                 575     


Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu Gly 
            580                 585                 590         


Ser Phe Gln Leu Glu Ile Arg Asn Val Asn Pro Asn Ile Pro Arg Tyr 
        595                 600                 605             


Arg Thr Arg Leu Arg Phe Glu Ile Asp Leu His Asn Lys Asp Lys Ser 
    610                 615                 620                 


Ile Leu Glu Asp Ile Gln Ser Thr Trp Lys Val Gly Lys Ile Tyr Asn 
625                 630                 635                 640 


Gln Gly Asp Ser Tyr Val Lys Leu Arg Val Thr Arg Phe Glu Asp Leu 
                645                 650                 655     


Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys 
            660                 665                 670         


Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Met Glu Asn 
        675                 680                 685             


Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile Lys 
    690                 695                 700                 


Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu Lys Lys Ala Phe Pro 
705                 710                 715                 720 


Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro Asn 
                725                 730                 735     


Leu Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly His Phe Gly Val 
            740                 745                 750         


Ile Leu Ala Lys Arg Arg Pro Ala Ser Pro Val Gln Val Arg Leu Arg 
        755                 760                 765             


Phe Ala Ile Gly Gln His Ile Arg Asp Lys Asn Leu Met Asn Ser Leu 
    770                 775                 780                 


Ile Thr Tyr Leu Gly Cys Gly Arg Thr Arg Glu Lys Asn Ile Ser Glu 
785                 790                 795                 800 


Lys Ser Trp Leu Glu Phe Glu Val Thr Lys Phe Ser Asp Ile Asn Asp 
                805                 810                 815     


Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys Leu 
            820                 825                 830         


Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu Lys 
        835                 840                 845             


Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu 
    850                 855                 860                 


Asn Met Asn Lys Gly Arg 
865                 870 


<210>  18
<211>  870
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized megaTAL PD-1.ITSM.ex5_RD4_CV23

<400>  18

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
225                 230                 235                 240 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                245                 250                 255     


Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            260                 265                 270         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        275                 280                 285             


Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr 
    290                 295                 300                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
305                 310                 315                 320 


Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu 
                325                 330                 335     


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
            340                 345                 350         


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln 
        355                 360                 365             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
    370                 375                 380                 


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
385                 390                 395                 400 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                405                 410                 415     


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
            420                 425                 430         


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
        435                 440                 445             


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
    450                 455                 460                 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
465                 470                 475                 480 


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
                485                 490                 495     


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala Gln 
            500                 505                 510         


Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu 
        515                 520                 525             


Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val Lys 
    530                 535                 540                 


Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg Arg 
545                 550                 555                 560 


Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Ser Arg Arg 
                565                 570                 575     


Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu Gly 
            580                 585                 590         


Ser Phe Gln Leu Glu Ile Arg Asn Val Asn Pro Asn Ile Pro Arg Tyr 
        595                 600                 605             


Arg Thr Arg Leu Arg Phe Glu Ile Asp Leu His Asn Lys Asp Lys Ser 
    610                 615                 620                 


Ile Leu Glu Asp Ile Gln Ser Thr Trp Lys Val Gly Lys Ile Tyr Asn 
625                 630                 635                 640 


Gln Gly Asp Ser Tyr Val Lys Leu Arg Val Thr Arg Phe Glu Asp Leu 
                645                 650                 655     


Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys 
            660                 665                 670         


Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Met Glu Asn 
        675                 680                 685             


Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile Lys 
    690                 695                 700                 


Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu Lys Lys Ala Phe Pro 
705                 710                 715                 720 


Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro Asn 
                725                 730                 735     


Leu Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly His Phe Gly Val 
            740                 745                 750         


Ile Leu Ala Lys Arg Arg Pro Ala Ser Pro Val Gln Val Arg Leu Arg 
        755                 760                 765             


Phe Ala Ile Gly Gln His Ile Arg Asp Lys Asn Leu Met Asn Ser Leu 
    770                 775                 780                 


Ile Thr Tyr Leu Gly Cys Gly Arg Ile Arg Glu Lys Asn Lys Ser Glu 
785                 790                 795                 800 


Lys Ser Trp Leu Glu Phe Glu Val Thr Lys Phe Ser Asp Ile Asn Asp 
                805                 810                 815     


Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys Leu 
            820                 825                 830         


Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu Lys 
        835                 840                 845             


Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu 
    850                 855                 860                 


Asn Met Asn Lys Gly Arg 
865                 870 


<210>  19
<211>  870
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized megaTAL PD-1.ITSM.ex5_RD5_CV23MK

<400>  19

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
225                 230                 235                 240 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                245                 250                 255     


Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            260                 265                 270         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        275                 280                 285             


Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr 
    290                 295                 300                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
305                 310                 315                 320 


Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu 
                325                 330                 335     


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
            340                 345                 350         


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln 
        355                 360                 365             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
    370                 375                 380                 


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
385                 390                 395                 400 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                405                 410                 415     


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
            420                 425                 430         


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
        435                 440                 445             


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
    450                 455                 460                 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
465                 470                 475                 480 


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
                485                 490                 495     


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala Gln 
            500                 505                 510         


Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu 
        515                 520                 525             


Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val Lys 
    530                 535                 540                 


Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg Arg 
545                 550                 555                 560 


Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Ser Arg Arg 
                565                 570                 575     


Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu Gly 
            580                 585                 590         


Ser Phe Gln Leu Glu Ile Arg Asn Val Asn Pro Asn Ile Pro Arg Tyr 
        595                 600                 605             


Arg Thr Arg Leu Arg Phe Glu Ile Asp Leu His Asn Lys Asp Lys Ser 
    610                 615                 620                 


Ile Leu Glu Asp Ile Gln Ser Thr Trp Lys Val Gly Lys Ile Tyr Asn 
625                 630                 635                 640 


Gln Gly Asp Ser Tyr Val Lys Leu Arg Val Thr Arg Phe Glu Asp Leu 
                645                 650                 655     


Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys 
            660                 665                 670         


Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Met Glu Asn 
        675                 680                 685             


Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile Lys 
    690                 695                 700                 


Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu Lys Lys Ala Phe Pro 
705                 710                 715                 720 


Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro Asn 
                725                 730                 735     


Leu Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly His Phe Gly Val 
            740                 745                 750         


Ile Leu Ala Lys Arg Arg Pro Ala Ser Pro Val Gln Val Arg Leu Arg 
        755                 760                 765             


Phe Met Ile Gly Gln His Ile Lys Asp Lys Asn Leu Met Asn Ser Leu 
    770                 775                 780                 


Ile Thr Tyr Leu Gly Cys Gly Arg Ile Arg Glu Lys Asn Lys Ser Glu 
785                 790                 795                 800 


Lys Ser Trp Leu Glu Phe Glu Val Thr Lys Phe Ser Asp Ile Asn Asp 
                805                 810                 815     


Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys Leu 
            820                 825                 830         


Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu Lys 
        835                 840                 845             


Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu 
    850                 855                 860                 


Asn Met Asn Lys Gly Arg 
865                 870 


<210>  20
<211>  841
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized megaTAL PD-1.ile3.exon1_RD1 B1

<400>  20

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala Gln Leu 
465                 470                 475                 480 


Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val 
                485                 490                 495     


Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val Lys Lys 
            500                 505                 510         


Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg Arg Ile 
        515                 520                 525             


Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly Gly Ser 
    530                 535                 540                 


Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp 
545                 550                 555                 560 


Ala Glu Gly Ser Phe Gly Leu Ser Ile Leu Asn Arg Asn Arg Gly Thr 
                565                 570                 575     


Ala Arg Tyr His Thr Arg Leu Ser Phe Ala Ile Val Leu His Asn Lys 
            580                 585                 590         


Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Ile 
        595                 600                 605             


Ile Thr Asn Asp Gly Asp Arg Tyr Val Arg Leu Arg Val Thr Arg Phe 
    610                 615                 620                 


Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Val 
625                 630                 635                 640 


Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val 
                645                 650                 655     


Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val 
            660                 665                 670         


Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu Lys Lys 
        675                 680                 685             


Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn 
    690                 695                 700                 


Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser 
705                 710                 715                 720 


Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala Arg Val Arg Val 
                725                 730                 735     


Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp Lys Asn Leu Met 
            740                 745                 750         


Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile Tyr Glu Gly Asn 
        755                 760                 765             


Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu Lys Phe Ser Asp 
    770                 775                 780                 


Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly 
785                 790                 795                 800 


Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile 
                805                 810                 815     


Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys 
            820                 825                 830         


Ile Lys Leu Asn Met Asn Lys Gly Arg 
        835                 840     


<210>  21
<211>  841
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized megaTAL PD-1.ile3.exon1_RD2_B1H8

<400>  21

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala Gln Leu 
465                 470                 475                 480 


Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val 
                485                 490                 495     


Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val Lys Lys 
            500                 505                 510         


Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg Arg Ile 
        515                 520                 525             


Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly Gly Ser 
    530                 535                 540                 


Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp 
545                 550                 555                 560 


Ala Glu Gly Ser Phe Gly Leu Ser Ile Leu Asn Arg Asn Arg Gly Thr 
                565                 570                 575     


Ala Arg Tyr His Thr Arg Leu Ser Phe Thr Ile Val Leu His Asn Lys 
            580                 585                 590         


Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Ile 
        595                 600                 605             


Ile Thr Asn Asp Gly Asp Arg Tyr Val Arg Leu Cys Val Thr Arg Phe 
    610                 615                 620                 


Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Val 
625                 630                 635                 640 


Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val 
                645                 650                 655     


Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Ala 
            660                 665                 670         


Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu Lys Lys 
        675                 680                 685             


Ala Phe Pro Glu Asn Ile Gly Lys Glu Arg Pro Leu Ile Asn Lys Asn 
    690                 695                 700                 


Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser 
705                 710                 715                 720 


Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala Arg Val Arg Val 
                725                 730                 735     


Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp Lys Asn Leu Met 
            740                 745                 750         


Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile Tyr Glu Gly Asn 
        755                 760                 765             


Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu Lys Phe Ser Asp 
    770                 775                 780                 


Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly 
785                 790                 795                 800 


Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile 
                805                 810                 815     


Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys 
            820                 825                 830         


Ile Lys Leu Asn Met Asn Lys Gly Arg 
        835                 840     


<210>  22
<211>  909
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized megaTAL PD-1.IgV.exon2_RD1_G5

<400>  22

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
465                 470                 475                 480 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                485                 490                 495     


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
            500                 505                 510         


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
        515                 520                 525             


Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Ser Ile 
    530                 535                 540                 


Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn 
545                 550                 555                 560 


Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp 
                565                 570                 575     


Ala Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val 
            580                 585                 590         


Asn Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg 
        595                 600                 605             


Val Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
    610                 615                 620                 


Gly Phe Ala Asp Ala Glu Gly Ser Phe Gln Leu Tyr Ile Ser Asn Val 
625                 630                 635                 640 


Asn Asn Asn Arg Ser Arg Tyr Arg Ala Arg Leu Arg Phe Ala Ile Glu 
                645                 650                 655     


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
            660                 665                 670         


Lys Val Gly Val Ile Asn Asn Ile Gly Asp Thr Ser Val Arg Leu Ser 
        675                 680                 685             


Val Gly Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
    690                 695                 700                 


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
705                 710                 715                 720 


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
                725                 730                 735     


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
            740                 745                 750         


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
        755                 760                 765             


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
    770                 775                 780                 


Gly Asp Gly Asn Phe Tyr Val His Leu Lys Lys Ser Gly Arg Thr Thr 
785                 790                 795                 800 


Arg Val Tyr Val Gln Leu Arg Phe Ser Ile Ala Gln His Ile Arg Asp 
                805                 810                 815     


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
            820                 825                 830         


Asn Glu Trp Asn Ala Ser Glu Arg Ser Ala Leu Glu Phe Arg Val Thr 
        835                 840                 845             


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
    850                 855                 860                 


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
865                 870                 875                 880 


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
                885                 890                 895     


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg 
            900                 905                 


<210>  23
<211>  909
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized megaTAL PD-1.IgV.exon2_RD1_PS3

<400>  23

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
465                 470                 475                 480 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                485                 490                 495     


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
            500                 505                 510         


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
        515                 520                 525             


Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Ser Ile 
    530                 535                 540                 


Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn 
545                 550                 555                 560 


Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp 
                565                 570                 575     


Ala Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val 
            580                 585                 590         


Asn Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg 
        595                 600                 605             


Val Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
    610                 615                 620                 


Gly Phe Ala Asp Ala Glu Gly Cys Phe Leu Leu His Ile Arg Asn Leu 
625                 630                 635                 640 


Asn Arg Thr Ser Thr Lys Tyr Arg Thr Arg Leu Ser Phe Glu Ile Glu 
                645                 650                 655     


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
            660                 665                 670         


Lys Val Gly Ile Ile Asn Asn Ile Gly Asn Arg Arg Val Arg Leu Ser 
        675                 680                 685             


Val Arg Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
    690                 695                 700                 


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
705                 710                 715                 720 


Ala Phe Ser Leu Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
                725                 730                 735     


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
            740                 745                 750         


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
        755                 760                 765             


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
    770                 775                 780                 


Gly Asp Gly Asn Phe Tyr Val His Leu Lys Lys Ser Gly Arg Thr Thr 
785                 790                 795                 800 


Arg Val Tyr Val Gln Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp 
                805                 810                 815     


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
            820                 825                 830         


Thr Glu Ser Asn Pro Ser Glu Arg Ser Asp Leu Glu Phe Arg Val Thr 
        835                 840                 845             


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
    850                 855                 860                 


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
865                 870                 875                 880 


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
                885                 890                 895     


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg 
            900                 905                 


<210>  24
<211>  236
<212>  PRT
<213>  Mus musculus

<400>  24

Met Ser Glu Pro Pro Arg Ala Glu Thr Phe Val Phe Leu Asp Leu Glu 
1               5                   10                  15      


Ala Thr Gly Leu Pro Asn Met Asp Pro Glu Ile Ala Glu Ile Ser Leu 
            20                  25                  30          


Phe Ala Val His Arg Ser Ser Leu Glu Asn Pro Glu Arg Asp Asp Ser 
        35                  40                  45              


Gly Ser Leu Val Leu Pro Arg Val Leu Asp Lys Leu Thr Leu Cys Met 
    50                  55                  60                  


Cys Pro Glu Arg Pro Phe Thr Ala Lys Ala Ser Glu Ile Thr Gly Leu 
65                  70                  75                  80  


Ser Ser Glu Ser Leu Met His Cys Gly Lys Ala Gly Phe Asn Gly Ala 
                85                  90                  95      


Val Val Arg Thr Leu Gln Gly Phe Leu Ser Arg Gln Glu Gly Pro Ile 
            100                 105                 110         


Cys Leu Val Ala His Asn Gly Phe Asp Tyr Asp Phe Pro Leu Leu Cys 
        115                 120                 125             


Thr Gly Leu Gln Arg Leu Gly Ala His Leu Pro Gln Asp Thr Val Cys 
    130                 135                 140                 


Leu Asp Thr Leu Pro Ala Leu Arg Gly Leu Asp Arg Ala His Ser His 
145                 150                 155                 160 


Gly Thr Arg Ala Gln Gly Arg Lys Ser Tyr Ser Leu Ala Ser Leu Phe 
                165                 170                 175     


His Arg Tyr Phe Gln Ala Glu Pro Ser Ala Ala His Ser Ala Glu Gly 
            180                 185                 190         


Asp Val His Thr Leu Leu Leu Ile Phe Leu His Arg Ala Pro Glu Leu 
        195                 200                 205             


Leu Ala Trp Ala Asp Glu Gln Ala Arg Ser Trp Ala His Ile Glu Pro 
    210                 215                 220                 


Met Tyr Val Pro Pro Asp Gly Pro Ser Leu Glu Ala 
225                 230                 235     


<210>  25
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  25
aatggtggca tactccgtct gc                                                22


<210>  26
<211>  11
<212>  DNA
<213>  Homo sapiens

<400>  26
tccgctagga a                                                            11


<210>  27
<211>  37
<212>  DNA
<213>  Homo sapiens

<400>  27
tccgctagga aagacaatgg tggcatactc cgtctgc                                37


<210>  28
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  28
aatggtggca tacaaccttt ta                                                22


<210>  29
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  29
tttccactta tactccgtct gc                                                22


<210>  30
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  30
ggcatgcaga tcccacaggc gc                                                22


<210>  31
<211>  14
<212>  DNA
<213>  Homo sapiens

<400>  31
ggtggggctg ctcc                                                         14


<210>  32
<211>  37
<212>  DNA
<213>  Homo sapiens

<400>  32
ggtggggctg ctccaggcat gcagatccca caggcgc                                37


<210>  33
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  33
ggcatgcaga tccaaccttt ta                                                22


<210>  34
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  34
tttccactta tcccacaggc gc                                                22


<210>  35
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  35
acgggcgtga cttccacatg ag                                                22


<210>  36
<211>  12
<212>  DNA
<213>  Homo sapiens

<400>  36
gtcacacaac tg                                                           12


<210>  37
<211>  38
<212>  DNA
<213>  Homo sapiens

<400>  37
gtcacacaac tgcccaacgg gcgtgacttc cacatgag                               38


<210>  38
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  38
acgggcgtga cttaaccttt ta                                                22


<210>  39
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  39
tttccactta cttccacatg ag                                                22


<210>  40
<211>  2650
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthesized PD-1 Exon5 RD5_CV23MK megaTAL mRNA

<400>  40
augggauccg ccccccccaa gaagaagcgc aagguggugg accugagaac ccugggguac       60

agccagcagc aacaggagaa gaucaaaccg aaggugcgca gcacgguggc ccagcaccau      120

gaagcccucg ugggucaugg auucacgcac gcccacaucg uggcccuguc gcagcauccg      180

gccgcccugg gcaccguggc ggugaccuac cagcacauca ucaccgcccu gcccgaagcc      240

acccacgagg acaucguggg agugggcaag cagugguccg gagcacgcgc ccuggaggcc      300

cugcugaccg acgccgggga acugcgcggc ccgccucugc agcuggauac cggccaacug      360

gucaagaucg ccaagagagg cggagugacc gcgauggagg ccguccacgc cucccggaac      420

gcucugaccg gugccccgcu caaucugacu ccggaccagg ugguggcuau cgccagcaac      480

ggaggaggaa aacaggcccu cgaaacagug cagaggcugc ugccuguccu uugucaagau      540

cacgggcuga cucccgacca ggucguggcc auugccagcc acgacggcgg caagcaggcu      600

uuggagacug ugcagcggcu ccugccagug cugugccaag aucacggucu gaccccagau      660

caggucgucg ccauugcuuc caacggaggc aaacaagcgc uggaaacggu ccaacgccug      720

cugccggugc uuugucagga ucacggccug accccugauc aggugguggc caucgcgucc      780

aauaacgggg ggaagcaggc acucgagacu guccagaggc uccugccugu gcucugccag      840

gaccacgggc ucacacccga ucaggucguc gcuaucgcgu cgcacgacgg uggaaagcag      900

gcccuggaaa ccgugcagcg ccuguugccg gugcuguguc aggaccaugg ccuuacuccg      960

gaucaggucg ucgcgaucgc aucuaauggu ggaggaaagc aggcccugga gacaguccag     1020

cgccugcucc cgguguugug ccaagaccau ggucuuaccc cugaccaggu ggucgcuauu     1080

gccucgaaca ucggcggaaa gcaagcccug gaaaccgugc agcgacuucu gccgguccug     1140

ugccaggauc auggauugac cccagaccag gugguggcga uugccagcaa caacggcggg     1200

aagcaagcgu uggaaaccgu ccagagacug cuuccugugc ugugccaaga ccacgguuug     1260

accccggacc aagucgucgc caucgcuucc aacaacggag ggaagcaggc acucgaaacu     1320

gugcaacggu ugcugcccgu gcucugucag gaucacggac ucaccccuga ucagguggug     1380

gccaucgcaa gcaacaucgg uggcaaacag gcucuggaaa cuguccaaag acugcugccc     1440

gugcuuugcc aggaccacgg acugacuccu gaccaagugg uggcaauugc cuccaacauc     1500

ggaggcaagc aagcgcucga auccaucgug gcgcagcuca gccggccaga ccccgcccug     1560

gccgcccuga cuaacgauca ccugguggcc cuggcgugcc ucggcggucg ccccgcuaug     1620

gacgcgguga agaaggggcu gccccacgcc cccgagcuca uucggcgggu gaaccgccgg     1680

aucggagaaa gaaccuccca ucggguggcc aucucgagau cacggcggga auccauuaac     1740

cccuggaucc ugacuggcuu ugccgacgcc gagggauccu uccagcucga aauccggaac     1800

gugaacccaa acaucccccg guaucgcacc agacugcggu ucgagaucga ccuucacaac     1860

aaggacaagu ccauucugga ggacauccag ucaaccugga aagugggaaa gaucuacaac     1920

cagggggacu cauacgugaa gcugcgggug acccgcuucg aagaucucaa agugaucauc     1980

gaccauuucg agaaguaccc ccugaucacu cagaagcuug gagacuacaa acuguucaag     2040

caggcauucu ccgugaugga gaacaaggag caccugaagg agaacgggau uaaggagcug     2100

gugcgaauua aggcgaaaau gaacugggga uugaacgaug agcugaagaa ggcguucccg     2160

gaaaacauuu ccaaggagcg cccgcucauc aacaagaaca ucccuaaucu gaaguggcuc     2220

gcgggguuua ccucuggcga cggccauuuc ggagugauuc ucgcaaagcg caggcccgcc     2280

agcccugugc aagugcggcu gcgguucaug aucggccagc acauuaagga caagaaccug     2340

augaacucgc ucaucaccua ccuuggaugc ggccggauuc gcgaaaagaa caagagcgag     2400

aaguccuggc uggaauuuga agugaccaag uucagcgaca ucaacgacaa gaucauuccg     2460

guguuccagg aaaacacccu gauuggcgug aagcuggagg acuucgagga uuggugcaag     2520

guggccaagc ucaucgaaga aaagaagcac cugacugaau ccggcuugga cgagaucaag     2580

aagauuaagc ugaauaugaa caagggaagg ugauagcgcg cuagccguac ggaaaaaaaa     2640

aaaaaaaaaa                                                            2650


<210>  41
<211>  2526
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthesized PD-1 Exon1 RD2_B1H8 megaTAL mRNA

<400>  41
augggaagcg ccccgccgaa gaagaagcgc aagguggugg aucugagaac ccugggauac       60

agccagcagc agcaggagaa gaucaagccg aagguccggu cuaccguggc ccagcaccau      120

gaggcccuug ugggccacgg cuucacacau gcacacaucg ucgcccuguc gcagcauccc      180

gccgcccugg ggaccguggc cgugaccuau caacacauca uuaccgcccu gccggaggcc      240

acccacgagg acaucguggg uguggggaag caguggagcg gagccagggc acucgaagcc      300

cuccucacug acgcuggaga acugcgcgga ccgccucucc agcuggacac cggacagcug      360

gugaaaaucg ccaagcgggg aggagugacc gccauggaag ccgugcacgc cucgaggaac      420

gcgcugacug gcgccccucu gaaccugacc ccugaucagg ucguggcuau cgccucaaac      480

aacgggggua agcaggcgcu ggagacagug caacgacuuc ugccagugcu uugucaggac      540

cauggucuga cccccgacca ggucgucgcc auugcaucca acaauggugg caagcaggca      600

cuggagacug uccagaggcu gcucccggug cugugccagg accacgggcu caccccggac      660

caaguggucg ccaucgccuc caacggagga ggaaaacaag cucuggagac ugugcaacgc      720

cugcugccug uguugugcca agaccacgga cugacgcccg aucagguggu ggcgaucgca      780

ucgaacaacg gaggaaagca agcgcuggaa accgugcagc gccuccugcc cguccucugc      840

caggaucacg gccugacucc ggaccaggug gucgcgaucg ccagcaauaa cggggggaag      900

caagcccucg agacugugca gcgguugcug cccgugcucu gccaagauca uggccuuacc      960

ccagaccaag ucguggccau ugcuuccaac aacgguggca aacaggcgcu cgaaaccguc     1020

cagcggcugu ugcccgugcu uugccaggau cacggacuca ccccugauca ggugguggca     1080

auugcgucca acaacggugg aaagcaggcc cuggaaacgg ugcagcggcu gcuuccgguc     1140

cugugucagg aucaugggcu gacucccgac caggucgucg ccauugcauc ccacgauggg     1200

gguaaacagg cccucgaaac agugcagaga cuccugccag uccugugcca agaccacgga     1260

cuuaccccgg aucagguggu ggccauagcc ucgaacggcg gcgggaaaca ggcucuggaa     1320

acugugcaaa gacuccuccc gguguugugu caagaccaug gacugacccc agaucaggug     1380

guggcuauug ccucuaacaa cggcggcaag caagcacucg aaagcaucgu ggcccaguug     1440

ucacgccccg accccgcacu ggcugcccug acgaaugacc aucugguggc gcuggccugc     1500

cugggaggga ggccagcgau ggaugcggug aagaagggac ugccccaugc uccggagcug     1560

auucggagag ugaauaggcg caucggagag agaacuucac aucggguggc cauuucuaga     1620

gugggcggca gcucccggcg cgaguccauu aaccccugga uccugaccgg cuuugccgac     1680

gccgaagggu ccuucggccu cucgauccug aaccggaacc gggguaccgc ucgguaccac     1740

accagacugu ccuucaccau cgugcugcac aacaaggaca agagcauccu cgaaaacauu     1800

cagucaacgu ggaagguggg aauuauuacu aacgacggcg acagauacgu gcgccugugc     1860

gugacccggu uugaggaccu gaaggucauu aucgaccacu ucgagaagua cccccucgug     1920

acucagaagc ugggagacua caagcuguuc aagcaggcgu ucucggugau ggaaaacaag     1980

gagcaccuga aggagaacgg caucaaggag cucgcccgga ucaaggccaa gaugaacugg     2040

ggccugaaug augaacucaa gaaggcguuc ccugaaaaca ucgguaaaga acggccccug     2100

aucaacaaga acaucccgaa cuucaagugg cuugccggau ucaccuccgg cgacggaucc     2160

uucuucgucc ggcugcgcaa guccaacgug aacgcgagag ugcgggugca auuggucuuu     2220

gaaaucucac agcacaucag ggacaagaau uugaugaacu cccucaucac cuaccugggu     2280

ugcggacaca ucuacgaagg caauaagucg gagcggagcu ggcugcaguu ucgcguggaa     2340

aaguucuccg acauuaacga caagaucauc ccaguguucc aggaaaacac ucugauuggc     2400

gugaagcuug aggauuucga ggacuggugc aagguggcca agcugauuga agagaagaag     2460

caccugaccg aguccggccu ggacgaaauc aagaaaauca agcugaacau gaacaaggga     2520

cgguga                                                                2526


<210>  42
<211>  2730
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthesized PD-1 Exon2 RD1_G5 megaTAL mRNA

<400>  42
augggaagcg ccccuccgaa gaagaagcgc aaggucgugg accucagaac ccuggguuac       60

ucccagcagc agcaggagaa aauuaagccg aaggugcgcu ccaccguggc ucaacaccac      120

gaggcccucg ugggccaugg auucacucac gcccauaucg uggcccuguc ccagcacccg      180

gccgcccugg gcaccguggc ggugaccuac cagcacauca ucaccgcgcu gccugaagcc      240

acccacgagg acaucgucgg uguggguaag cagugguccg gagccagagc ccuggaggcu      300

cugcugaccg acgccggaga acucagaggc ccgccucugc agcuggacac cggacagcug      360

gugaagauag ccaagagagg cggcgugacc gccauggaag ccgugcaugc gucccgcaac      420

gcacugaccg gggccccccu gaaccugacu ccagaccaag ugguggcuau cgccagcaac      480

aauggaggaa agcaagccuu ggaaaccgug cagcggcugc ucccgguccu uugccaagac      540

cacggccuga cacccgauca ggugguggca aucgcaucga auggcggcgg gaagcaggcc      600

cucgagacug ugcagaggcu ccugccugug cugugccagg accaugggcu gaccccagac      660

cagguggucg caaucgccuc gcacgacgga ggcaagcaag cccuggaaac uguccagcgc      720

cugcucccug uccuguguca agaucauggg cucacuccug aucaggucgu cgccaucgcc      780

ucgaacauug guggcaagca ggcgcucgaa accguccagc gguugcugcc agugcuuugc      840

caggaccaug gucugacccc cgaucaagug gucgcgauug ccucacacga uggcgguaag      900

caggcuuugg aaaccgugca acggcuguug ccuguccucu gccaggacca cggcuugacu      960

cccgaccagg ugguggccau agccucaaac aucggaggga aacaagcccu cgaaaccguc     1020

cagaggcugc ugccgguguu gugccaggau cacggauuga ccccagacca gguggucgcc     1080

auugcuuccc acgauggggg aaagcaggcc cuggagacug ugcagcgccu ccuucccguc     1140

cugugucagg aucauggacu uacccccgac caagucgugg cgauugcuuc caauaucgga     1200

ggcaaacagg cccuugaaac agugcagcgc cuguugccgg ugcucugcca agaucacgga     1260

cugaccccug accagguggu ggcgaucgcg ucaaauaucg gcggcaagca ggcacuggag     1320

acaguccaga gacuccugcc gguccucugc caggaccacg gucuuacucc ugaccaagug     1380

guggcuaucg caucccauga uggugggaaa caggcucuug aaacugugca acgccugcug     1440

cccgugcugu gccaagacca cggacugacu ccggaccagg ucguggccau cgcuucaaac     1500

ggagggggaa aacaggcacu ugaaacggug cagagacugc ugccuguccu uugucaggac     1560

cacggguuga ccccggacca ggucguggcu auugccucga acaacggggg gaagcaagcg     1620

cucgagucca uuguggccca gcugagccgg ccugaucccg cacuggccgc gcugaccaac     1680

gaucaccugg uggcccucgc cugucugggc ggacggccgg ccauggacgc cgugaagaag     1740

ggacugccgc acgcgcccga gcugauccgc cgcgugaaca ggcggauugg agaacgcacc     1800

ucccaccggg uggccaucuc gagaguggga ggauccucua gacgggaguc cauuaacccg     1860

uggauccuga cuggauucgc cgacgccgag ggguccuucc agcuguacau cuccaacgug     1920

aacaacaacc gcagcagaua cagggcccgg cugcgguucg cgaucgaacu gcacaauaag     1980

gacaagagca uccucgagaa cauucagucc acuuggaagg ugggcgugau uaacaacauc     2040

ggcgauacca gcgugcggcu cuccgugggg cgguucgaag aucucaaggu gaucaucgac     2100

cacuucgaga aguacccgcu gaucacccag aagcugggag acuacaagcu guucaagcaa     2160

gcuuucagcg ugauggaaaa caaggaacau cugaaagaga acgguaucaa ggaacuggug     2220

cggauuaagg ccaagaugaa cuggggucug aacgaugaac ugaagaaggc guucccggag     2280

aauaucagca aggagcgccc ccugauuaac aaaaacaucc caaaccucaa guggcucgcc     2340

ggcuuuacuu ccggagaugg uaacuucuac gugcaccuga agaaguccgg aagaaccacc     2400

cgggucuacg ugcagcugag guucuccauc gcccagcaca uccgggacaa gaacuugaug     2460

aacucccuga ucaccuaccu cgguugcgga uacaucaacg aauggaacgc cagcgagaga     2520

ucggcccugg aguucagggu gacuaaguuc uccgacauca acgacaagau uauccccgug     2580

uuucaggaaa auacccucau cggcgugaag cuggaggacu uugaggacug gugcaaggug     2640

gccaagcuga ucgaggaaaa gaaacaucug accgagagcg gccuggacga aaucaagaag     2700

auuaagcuga acaugaacaa gggacgauga                                      2730


<210>  43
<211>  711
<212>  RNA
<213>  Mus musculus

<400>  43
augucugagc caccucgggc ugagaccuuu guauuccugg accuagaagc cacugggcuc       60

ccaaacaugg acccugagau ugcagagaua ucccuuuuug cuguucaccg cucuucccug      120

gagaacccag aacgggauga uucugguucc uuggugcugc cccguguucu ggacaagcuc      180

acacugugca ugugcccgga gcgccccuuu acugccaagg ccagugagau uacugguuug      240

agcagcgaaa gccugaugca cugcgggaag gcugguuuca auggcgcugu gguaaggaca      300

cugcagggcu uccuaagccg ccaggagggc cccaucugcc uuguggccca caauggcuuc      360

gauuaugacu ucccacugcu gugcacgggg cuacaacguc ugggugccca ucugccccaa      420

gacacugucu gccuggacac acugccugca uugcggggcc uggaccgugc ucacagccac      480

ggcaccaggg cucaaggccg caaaagcuac agccuggcca gucucuucca ccgcuacuuc      540

caggcugaac ccagugcugc ccauucagca gaaggugaug ugcacacccu gcuucugauc      600

uuccugcauc gugcuccuga gcugcucgcc ugggcagaug agcaggcccg cagcugggcu      660

cauauugagc ccauguacgu gccaccugau gguccaagcc ucgaagccug a               711


<210>  44
<211>  7601
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized rAAV PD-1 Exon5_MND-BFP construct

<400>  44
cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc       60

tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc      120

actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagaattca      180

gtccagggct ctgtcctgca cctggggaat ggtgaccggc atctctgtcc tctagctctg      240

gaagcacccc agcccctcta gtctgccctc acccctgacc ctgaccctcc accctgaccc      300

cgtcctaacc cctgaccttt gtgcccttcc agagagaagg gcagaagtgc ccacagccca      360

ccccagcccc tcacccaggc cagccggcca gttccaaacc ctggtggttg gtgtcgtggg      420

cggcctgctg ggcagcctgg tgctgctagt ctgggtcctg gccgtcatct gctcccgggc      480

cgcacgaggt aacgtcatcc cagcccctcg gcctgccctg ccctaaccct gctggcggcc      540

ctcactcccg cctccccttc ctccaccctt ccctcacccc accccacctc cccccatctc      600

cccgccaggc taagtccctg atgaaggccc ctggactaag accccccacc taggagcacg      660

gctcagggtc ggcctggtga ccccaagtgt gtttctctgc agggacaata ggagccaggc      720

gcaccggcca gcccctggtg agtctcactc ttttcctgca tgatccactg tgccttcctt      780

cctgggtggg cagaggtgga aggacaggct gggaccacac ggcctgcagg actcacattc      840

tattatagcc aggaccccac ctccccagcc cccaggcagc aacctcaatc cctaaagcca      900

tgatctgggg ccccagccca cctgcggtct ccgggggtgc ccggcccatg tgtgtgcctg      960

cctgcggtct ccaggggtgc ctggcccacg cgtgtgcccg cctgcggtct ctgggggtgc     1020

ccggcccaca tatgtgcctg cctgcggtct ccaggtgtgc ccggcccatg cgtgtgccca     1080

cctgcgaggg cgtggggtgg gcttggtcat ttcttatctt acattggaga caggagagct     1140

tgaaaagtca cattttggaa tcctaaatct gcaagaatgc cagggacatt tcagaggggg     1200

acattgagcc agagaggagg ggtggtgtcc ccagatcaca cagagggcag tggtgggaca     1260

gctcagggta agcagctcat agtggggggc ccaggttcgg tgccggtact gcagccaggc     1320

tgtggagccg cgggcctcct tcctgcggtg ggccgtgggg ctgactccct ctccctttct     1380

cctcaaagaa ggaggacccc tcagccgtgc ctgtgttctc tgtggactat ggggagctgg     1440

atttccagtg gcgagagaag accccggagc cccccgtgcc ctgtgtccct gagcagacgg     1500

agtaatgcat gaacagagaa acaggagaat atgggccaaa caggatatct gtggtaagca     1560

gttcctgccc cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga     1620

tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc     1680

ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc     1740

tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc     1800

gcgcttctgc tccccgagct ctatataagc agagctcgtt tagtgaaccg tcagatcgcc     1860

tggagacgcc atccacgctg ttttgacttc catagaagga tctcgaggcc accatggcta     1920

gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc     1980

atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga     2040

gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct     2100

tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc     2160

agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaggac gggggcgtgc     2220

tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca     2280

gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg     2340

ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc     2400

tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga     2460

aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa     2520

tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact     2580

gcgacctccc tagcaaactg gggcacaagc taaattgaaa gctttgcttt atttgtgaaa     2640

tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gtttaacaac     2700

aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagt     2760

cgacccacca ttgtctttcc tagcggaatg ggcacctcat cccccgcccg caggggctca     2820

gctgacggcc ctcggagtgc ccagccactg aggcctgagg atggacactg ctcttggccc     2880

ctctgaccgg cttccttggc caccagtgtt ctgcagaccc tccaccatga gcccgggtca     2940

gcgcatttcc tcaggagaag caggcagggt gcaggccatt gcaggccgtc caggggctga     3000

gctgcctggg ggcgaccggg gctccagcct gcacctgcac caggcacagc cccaccacag     3060

gactcatgtc tcaatgccca cagtgagccc aggcagcagg tgtcaccgtc ccctacaggg     3120

agggccagat gcagtcactg cttcaggtcc tgccagcaca gagctgcctg cgtccagctc     3180

cctgaatctc tgctgctgct gctgctgctg ctgctgctgc ctgcggcccg gggctgaagg     3240

cgccgtggcc ctgcctgacg ccccggagcc tcctgcctga acttgggggc tggttggaga     3300

tggccttgga gcagccaagg tgcccctggc agtggcatcc cgaaacgccc tggacgcagg     3360

gcccaagact gggcacagga gtgggaggta catggggctg gggactcccc aggagttatc     3420

tgctccctgc aggcctagag aagtttcagg gaaggtcaga agagctcctg gctgtggtgg     3480

gcagggcagg aaacccctcc acctttacac atgcccaggc agcacctcag gccctttgtg     3540

gggcagggaa gctgaggcag taagcgggca ggcagagctg gaggcctttc aggcccagcc     3600

agcactctgg cctcctgccg ccgcattcca ccccagcccc tcacaccact cgggagaggg     3660

acatcctacg gtcccaaggt caggagggca gggctggggt tgactcaggc ccctcccagc     3720

tgtggccacc tgggtgttgg gagggcagaa gtgcaggcac ctagggcccc ccatgtgccc     3780

accctgggag ctctccttgg aacccattcc tgaaattatt taaaggggtt ggccggacta     3840

gttacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg     3900

agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg     3960

cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc cagctggcgt     4020

aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa     4080

tggcgattcc gttgcaatgg ctggcggtaa tattgttctg gatattacca gcaaggccga     4140

tagtttgagt tcttctactc aggcaagtga tgttattact aatcaaagaa gtattgcgac     4200

aacggttaat ttgcgtgatg gacagactct tttactcggt ggcctcactg attataaaaa     4260

cacttctcag gattctggcg taccgttcct gtctaaaatc cctttaatcg gcctcctgtt     4320

tagctcccgc tctgattcta acgaggaaag cacgttatac gtgctcgtca aagcaaccat     4380

agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga     4440

ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg     4500

ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat     4560

ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg     4620

ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata     4680

gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt     4740

tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat     4800

ttaacgcgaa ttttaacaaa atattaacgt ttacaattta aatatttgct tatacaatct     4860

tcctgttttt ggggcttttc tgattatcaa ccggggtaca tatgattgac atgctagttt     4920

tacgattacc gttcatcgat tctcttgttt gctccagact ctcaggcaat gacctgatag     4980

cctttgtaga gacctctcaa aaatagctac cctctccggc atgaatttat cagctagaac     5040

ggttgaatat catattgatg gtgatttgac tgtctccggc ctttctcacc cgtttgaatc     5100

tttacctaca cattactcag gcattgcatt taaaatatat gagggttcta aaaattttta     5160

tccttgcgtt gaaataaagg cttctcccgc aaaagtatta cagggtcata atgtttttgg     5220

tacaaccgat ttagctttat gctctgaggc tttattgctt aattttgcta attctttgcc     5280

ttgcctgtat gatttattgg atgttggaat cgcctgatgc ggtattttct ccttacgcat     5340

ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca     5400

tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg     5460

ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg     5520

ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta     5580

taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat     5640

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg     5700

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa     5760

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac     5820

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac     5880

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt     5940

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc     6000

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca     6060

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc     6120

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     6180

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     6240

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     6300

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     6360

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     6420

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     6480

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     6540

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     6600

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     6660

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     6720

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     6780

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     6840

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     6900

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     6960

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     7020

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     7080

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     7140

tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg     7200

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     7260

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     7320

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     7380

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     7440

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     7500

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata     7560

cgcaaaccgc ctctccccgc gcgttggccg attcattaat g                         7601


<210>  45
<211>  1325
<212>  DNA
<213>  Homo sapiens

<400>  45
agtccagggc tctgtcctgc acctggggaa tggtgaccgg catctctgtc ctctagctct       60

ggaagcaccc cagcccctct agtctgccct cacccctgac cctgaccctc caccctgacc      120

ccgtcctaac ccctgacctt tgtgcccttc cagagagaag ggcagaagtg cccacagccc      180

accccagccc ctcacccagg ccagccggcc agttccaaac cctggtggtt ggtgtcgtgg      240

gcggcctgct gggcagcctg gtgctgctag tctgggtcct ggccgtcatc tgctcccggg      300

ccgcacgagg taacgtcatc ccagcccctc ggcctgccct gccctaaccc tgctggcggc      360

cctcactccc gcctcccctt cctccaccct tccctcaccc caccccacct ccccccatct      420

ccccgccagg ctaagtccct gatgaaggcc cctggactaa gaccccccac ctaggagcac      480

ggctcagggt cggcctggtg accccaagtg tgtttctctg cagggacaat aggagccagg      540

cgcaccggcc agcccctggt gagtctcact cttttcctgc atgatccact gtgccttcct      600

tcctgggtgg gcagaggtgg aaggacaggc tgggaccaca cggcctgcag gactcacatt      660

ctattatagc caggacccca cctccccagc ccccaggcag caacctcaat ccctaaagcc      720

atgatctggg gccccagccc acctgcggtc tccgggggtg cccggcccat gtgtgtgcct      780

gcctgcggtc tccaggggtg cctggcccac gcgtgtgccc gcctgcggtc tctgggggtg      840

cccggcccac atatgtgcct gcctgcggtc tccaggtgtg cccggcccat gcgtgtgccc      900

acctgcgagg gcgtggggtg ggcttggtca tttcttatct tacattggag acaggagagc      960

ttgaaaagtc acattttgga atcctaaatc tgcaagaatg ccagggacat ttcagagggg     1020

gacattgagc cagagaggag gggtggtgtc cccagatcac acagagggca gtggtgggac     1080

agctcagggt aagcagctca tagtgggggg cccaggttcg gtgccggtac tgcagccagg     1140

ctgtggagcc gcgggcctcc ttcctgcggt gggccgtggg gctgactccc tctccctttc     1200

tcctcaaaga aggaggaccc ctcagccgtg cctgtgttct ctgtggacta tggggagctg     1260

gatttccagt ggcgagagaa gaccccggag ccccccgtgc cctgtgtccc tgagcagacg     1320

gagta                                                                 1325


<210>  46
<211>  1072
<212>  DNA
<213>  Homo sapiens

<400>  46
ccaccattgt ctttcctagc ggaatgggca cctcatcccc cgcccgcagg ggctcagctg       60

acggccctcg gagtgcccag ccactgaggc ctgaggatgg acactgctct tggcccctct      120

gaccggcttc cttggccacc agtgttctgc agaccctcca ccatgagccc gggtcagcgc      180

atttcctcag gagaagcagg cagggtgcag gccattgcag gccgtccagg ggctgagctg      240

cctgggggcg accggggctc cagcctgcac ctgcaccagg cacagcccca ccacaggact      300

catgtctcaa tgcccacagt gagcccaggc agcaggtgtc accgtcccct acagggaggg      360

ccagatgcag tcactgcttc aggtcctgcc agcacagagc tgcctgcgtc cagctccctg      420

aatctctgct gctgctgctg ctgctgctgc tgctgcctgc ggcccggggc tgaaggcgcc      480

gtggccctgc ctgacgcccc ggagcctcct gcctgaactt gggggctggt tggagatggc      540

cttggagcag ccaaggtgcc cctggcagtg gcatcccgaa acgccctgga cgcagggccc      600

aagactgggc acaggagtgg gaggtacatg gggctgggga ctccccagga gttatctgct      660

ccctgcaggc ctagagaagt ttcagggaag gtcagaagag ctcctggctg tggtgggcag      720

ggcaggaaac ccctccacct ttacacatgc ccaggcagca cctcaggccc tttgtggggc      780

agggaagctg aggcagtaag cgggcaggca gagctggagg cctttcaggc ccagccagca      840

ctctggcctc ctgccgccgc attccacccc agcccctcac accactcggg agagggacat      900

cctacggtcc caaggtcagg agggcagggc tggggttgac tcaggcccct cccagctgtg      960

gccacctggg tgttgggagg gcagaagtgc aggcacctag ggccccccat gtgcccaccc     1020

tgggagctct ccttggaacc cattcctgaa attatttaaa ggggttggcc gg             1072


<210>  47
<211>  7601
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized rAAV PD-1 Exon5_MND-BFP 3'-Homology Arm SNP construct

<400>  47
cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc       60

tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc      120

actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagaattca      180

gtccagggct ctgtcctgca cctggggaat ggtgaccggc atctctgtcc tctagctctg      240

gaagcacccc agcccctcta gtctgccctc acccctgacc ctgaccctcc accctgaccc      300

cgtcctaacc cctgaccttt gtgcccttcc agagagaagg gcagaagtgc ccacagccca      360

ccccagcccc tcacccaggc cagccggcca gttccaaacc ctggtggttg gtgtcgtggg      420

cggcctgctg ggcagcctgg tgctgctagt ctgggtcctg gccgtcatct gctcccgggc      480

cgcacgaggt aacgtcatcc cagcccctcg gcctgccctg ccctaaccct gctggcggcc      540

ctcactcccg cctccccttc ctccaccctt ccctcacccc accccacctc cccccatctc      600

cccgccaggc taagtccctg atgaaggccc ctggactaag accccccacc taggagcacg      660

gctcagggtc ggcctggtga ccccaagtgt gtttctctgc agggacaata ggagccaggc      720

gcaccggcca gcccctggtg agtctcactc ttttcctgca tgatccactg tgccttcctt      780

cctgggtggg cagaggtgga aggacaggct gggaccacac ggcctgcagg actcacattc      840

tattatagcc aggaccccac ctccccagcc cccaggcagc aacctcaatc cctaaagcca      900

tgatctgggg ccccagccca cctgcggtct ccgggggtgc ccggcccatg tgtgtgcctg      960

cctgcggtct ccaggggtgc ctggcccacg cgtgtgcccg cctgcggtct ctgggggtgc     1020

ccggcccaca tatgtgcctg cctgcggtct ccaggtgtgc ccggcccatg cgtgtgccca     1080

cctgcgaggg cgtggggtgg gcttggtcat ttcttatctt acattggaga caggagagct     1140

tgaaaagtca cattttggaa tcctaaatct gcaagaatgc cagggacatt tcagaggggg     1200

acattgagcc agagaggagg ggtggtgtcc ccagatcaca cagagggcag tggtgggaca     1260

gctcagggta agcagctcat agtggggggc ccaggttcgg tgccggtact gcagccaggc     1320

tgtggagccg cgggcctcct tcctgcggtg ggccgtgggg ctgactccct ctccctttct     1380

cctcaaagaa ggaggacccc tcagccgtgc ctgtgttctc tgtggactat ggggagctgg     1440

atttccagtg gcgagagaag accccggagc cccccgtgcc ctgtgtccct gagcagacgg     1500

agtaatgcat gaacagagaa acaggagaat atgggccaaa caggatatct gtggtaagca     1560

gttcctgccc cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga     1620

tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc     1680

ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc     1740

tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc     1800

gcgcttctgc tccccgagct ctatataagc agagctcgtt tagtgaaccg tcagatcgcc     1860

tggagacgcc atccacgctg ttttgacttc catagaagga tctcgaggcc accatggcta     1920

gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc     1980

atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga     2040

gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct     2100

tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc     2160

agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaggac gggggcgtgc     2220

tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca     2280

gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg     2340

ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc     2400

tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga     2460

aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa     2520

tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact     2580

gcgacctccc tagcaaactg gggcacaagc taaattgaaa gctttgcttt atttgtgaaa     2640

tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gtttaacaac     2700

aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagt     2760

cgacccacca ttgtctttcc tagcggaatg ggcacctcat cccccgcccg caggggctca     2820

gccgacggcc ctcggagtgc ccagccactg aggcctgagg atggacactg ctcttggccc     2880

ctctgaccgg cttccttggc caccagtgtt ctgcagaccc tccaccatga gcccgggtca     2940

gcgcatttcc tcaggagaag caggcagggt gcaggccatt gcaggccgtc caggggctga     3000

gctgcctggg ggcgaccggg gctccagcct gcacctgcac caggcacagc cccaccacag     3060

gactcatgtc tcaatgccca cagtgagccc aggcagcagg tgtcaccgtc ccctacaggg     3120

agggccagat gcagtcactg cttcaggtcc tgccagcaca gagctgcctg cgtccagctc     3180

cctgaatctc tgctgctgct gctgctgctg ctgctgctgc ctgcggcccg gggctgaagg     3240

cgccgtggcc ctgcctgacg ccccggagcc tcctgcctga acttgggggc tggttggaga     3300

tggccttgga gcagccaagg tgcccctggc agtggcatcc cgaaacgccc tggacgcagg     3360

gcccaagact gggcacagga gtgggaggta catggggctg gggactcccc aggagttatc     3420

tgctccctgc aggcctagag aagtttcagg gaaggtcaga agagctcctg gctgtggtgg     3480

gcagggcagg aaacccctcc acctttacac atgcccaggc agcacctcag gccctttgtg     3540

gggcagggaa gctgaggcag taagcgggca ggcagagctg gaggcctttc aggcccagcc     3600

agcactctgg cctcctgccg ccgcattcca ccccagcccc tcacaccact cgggagaggg     3660

acatcctacg gtcccaaggt caggagggca gggctggggt tgactcaggc ccctcccagc     3720

tgtggccacc tgggtgttgg gagggcagaa gtgcaggcac ctagggcccc ccatgtgccc     3780

accctgggag ctctccttgg aacccattcc tgaaattatt taaaggggtt ggccggacta     3840

gttacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg     3900

agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg     3960

cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc cagctggcgt     4020

aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa     4080

tggcgattcc gttgcaatgg ctggcggtaa tattgttctg gatattacca gcaaggccga     4140

tagtttgagt tcttctactc aggcaagtga tgttattact aatcaaagaa gtattgcgac     4200

aacggttaat ttgcgtgatg gacagactct tttactcggt ggcctcactg attataaaaa     4260

cacttctcag gattctggcg taccgttcct gtctaaaatc cctttaatcg gcctcctgtt     4320

tagctcccgc tctgattcta acgaggaaag cacgttatac gtgctcgtca aagcaaccat     4380

agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga     4440

ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg     4500

ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat     4560

ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg     4620

ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata     4680

gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt     4740

tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat     4800

ttaacgcgaa ttttaacaaa atattaacgt ttacaattta aatatttgct tatacaatct     4860

tcctgttttt ggggcttttc tgattatcaa ccggggtaca tatgattgac atgctagttt     4920

tacgattacc gttcatcgat tctcttgttt gctccagact ctcaggcaat gacctgatag     4980

cctttgtaga gacctctcaa aaatagctac cctctccggc atgaatttat cagctagaac     5040

ggttgaatat catattgatg gtgatttgac tgtctccggc ctttctcacc cgtttgaatc     5100

tttacctaca cattactcag gcattgcatt taaaatatat gagggttcta aaaattttta     5160

tccttgcgtt gaaataaagg cttctcccgc aaaagtatta cagggtcata atgtttttgg     5220

tacaaccgat ttagctttat gctctgaggc tttattgctt aattttgcta attctttgcc     5280

ttgcctgtat gatttattgg atgttggaat cgcctgatgc ggtattttct ccttacgcat     5340

ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca     5400

tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg     5460

ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg     5520

ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta     5580

taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat     5640

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg     5700

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa     5760

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac     5820

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac     5880

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt     5940

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc     6000

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca     6060

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc     6120

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     6180

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     6240

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     6300

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     6360

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     6420

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     6480

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     6540

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     6600

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     6660

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     6720

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     6780

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     6840

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     6900

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     6960

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     7020

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     7080

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     7140

tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg     7200

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     7260

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     7320

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     7380

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     7440

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     7500

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata     7560

cgcaaaccgc ctctccccgc gcgttggccg attcattaat g                         7601


<210>  48
<211>  7601
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized rAAV PD-1 Exon5_MND-BFP 5'-Homology Arm SNP construct

<400>  48
cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc       60

tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc      120

actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagaattca      180

gtccagggct ctgtcctgca cctggggaat ggtgaccggc atctctgtcc tctagctctg      240

gaagcacccc agcccctcta gtctgccctc acccctgacc ctgaccctcc accctgaccc      300

cgtcctaacc cctgaccttt gtgcccttcc agagagaagg gcagaagtgc ccacagccca      360

ccccagcccc tcacccaggc cagccggcca gttccaaacc ctggtggttg gtgtcgtggg      420

cggcctgctg ggcagcctgg tgctgctagt ctgggtcctg gccgtcatct gctcccgggc      480

cgcacgaggt aacgtcatcc cagcccctcg gcctgccctg ccctaaccct gctggcggcc      540

ctcactcccg cctccccttc ctccaccctt ccctcacccc accccacctc cccccatctc      600

cccgccaggc taagtccctg atgaaggccc ctggactaag accccccacc taggagcacg      660

gctcagggtc ggcctggtga ccccaagtgt gtttctctgc agggacaata ggagccaggc      720

gcaccggcca gcccctggtg agtctcactc ttttcctgca tgatccactg tgccttcctt      780

cctgggtggg cagaggtgga aggacaggct gggaccacac ggcctgcagg actcacattc      840

tattatagcc aggaccccac ctccccagcc cccaggcagc aacctcaatc cctaaagcca      900

tgatctgggg ccccagccca cctgcggtct ccgggggtgc ccggcccatg tgtgtgcctg      960

cctgcggtct ccaggggtgc ctggcccacg cgtgtgcccg cctgcggtct ctgggggtgc     1020

ccggcccaca tatgtgcctg cctgcggtct ccaggtgtgc ccggcccatg cgtgtgccca     1080

cctgcgaggg cgtggggtgg gcttggtcat ttcttatctt acattggaga caggagagct     1140

tgaaaagtca cattttggaa tcctaaatct gcaagaatgc cagggacatt tcagaggggg     1200

acattgagcc agagaggagg ggtggtgtcc ccagatcaca cagagggcag tggtgggaca     1260

gctcagggta agcagctcgt agtggggggc ccaggttcgg tgccggtact gcagccaggc     1320

tgtggagccg cgggcctcct tcctgcggtg ggccgtgggg ctgactccct ctccctttct     1380

cctcaaagaa ggaggacccc tcagccgtgc ctgtgttctc tgtggactat ggggagctgg     1440

atttccagtg gcgagagaag accccggagc cccccgtgcc ctgtgtccct gagcagacgg     1500

agtaatgcat gaacagagaa acaggagaat atgggccaaa caggatatct gtggtaagca     1560

gttcctgccc cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga     1620

tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc     1680

ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc     1740

tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc     1800

gcgcttctgc tccccgagct ctatataagc agagctcgtt tagtgaaccg tcagatcgcc     1860

tggagacgcc atccacgctg ttttgacttc catagaagga tctcgaggcc accatggcta     1920

gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc     1980

atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga     2040

gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct     2100

tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc     2160

agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaggac gggggcgtgc     2220

tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca     2280

gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg     2340

ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc     2400

tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga     2460

aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa     2520

tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact     2580

gcgacctccc tagcaaactg gggcacaagc taaattgaaa gctttgcttt atttgtgaaa     2640

tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gtttaacaac     2700

aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagt     2760

cgacccacca ttgtctttcc tagcggaatg ggcacctcat cccccgcccg caggggctca     2820

gctgacggcc ctcggagtgc ccagccactg aggcctgagg atggacactg ctcttggccc     2880

ctctgaccgg cttccttggc caccagtgtt ctgcagaccc tccaccatga gcccgggtca     2940

gcgcatttcc tcaggagaag caggcagggt gcaggccatt gcaggccgtc caggggctga     3000

gctgcctggg ggcgaccggg gctccagcct gcacctgcac caggcacagc cccaccacag     3060

gactcatgtc tcaatgccca cagtgagccc aggcagcagg tgtcaccgtc ccctacaggg     3120

agggccagat gcagtcactg cttcaggtcc tgccagcaca gagctgcctg cgtccagctc     3180

cctgaatctc tgctgctgct gctgctgctg ctgctgctgc ctgcggcccg gggctgaagg     3240

cgccgtggcc ctgcctgacg ccccggagcc tcctgcctga acttgggggc tggttggaga     3300

tggccttgga gcagccaagg tgcccctggc agtggcatcc cgaaacgccc tggacgcagg     3360

gcccaagact gggcacagga gtgggaggta catggggctg gggactcccc aggagttatc     3420

tgctccctgc aggcctagag aagtttcagg gaaggtcaga agagctcctg gctgtggtgg     3480

gcagggcagg aaacccctcc acctttacac atgcccaggc agcacctcag gccctttgtg     3540

gggcagggaa gctgaggcag taagcgggca ggcagagctg gaggcctttc aggcccagcc     3600

agcactctgg cctcctgccg ccgcattcca ccccagcccc tcacaccact cgggagaggg     3660

acatcctacg gtcccaaggt caggagggca gggctggggt tgactcaggc ccctcccagc     3720

tgtggccacc tgggtgttgg gagggcagaa gtgcaggcac ctagggcccc ccatgtgccc     3780

accctgggag ctctccttgg aacccattcc tgaaattatt taaaggggtt ggccggacta     3840

gttacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg     3900

agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg     3960

cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc cagctggcgt     4020

aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa     4080

tggcgattcc gttgcaatgg ctggcggtaa tattgttctg gatattacca gcaaggccga     4140

tagtttgagt tcttctactc aggcaagtga tgttattact aatcaaagaa gtattgcgac     4200

aacggttaat ttgcgtgatg gacagactct tttactcggt ggcctcactg attataaaaa     4260

cacttctcag gattctggcg taccgttcct gtctaaaatc cctttaatcg gcctcctgtt     4320

tagctcccgc tctgattcta acgaggaaag cacgttatac gtgctcgtca aagcaaccat     4380

agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga     4440

ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg     4500

ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat     4560

ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg     4620

ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata     4680

gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt     4740

tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat     4800

ttaacgcgaa ttttaacaaa atattaacgt ttacaattta aatatttgct tatacaatct     4860

tcctgttttt ggggcttttc tgattatcaa ccggggtaca tatgattgac atgctagttt     4920

tacgattacc gttcatcgat tctcttgttt gctccagact ctcaggcaat gacctgatag     4980

cctttgtaga gacctctcaa aaatagctac cctctccggc atgaatttat cagctagaac     5040

ggttgaatat catattgatg gtgatttgac tgtctccggc ctttctcacc cgtttgaatc     5100

tttacctaca cattactcag gcattgcatt taaaatatat gagggttcta aaaattttta     5160

tccttgcgtt gaaataaagg cttctcccgc aaaagtatta cagggtcata atgtttttgg     5220

tacaaccgat ttagctttat gctctgaggc tttattgctt aattttgcta attctttgcc     5280

ttgcctgtat gatttattgg atgttggaat cgcctgatgc ggtattttct ccttacgcat     5340

ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca     5400

tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg     5460

ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg     5520

ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta     5580

taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat     5640

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg     5700

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa     5760

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac     5820

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac     5880

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt     5940

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc     6000

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca     6060

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc     6120

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     6180

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     6240

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     6300

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     6360

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     6420

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     6480

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     6540

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     6600

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     6660

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     6720

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     6780

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     6840

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     6900

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     6960

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     7020

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     7080

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     7140

tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg     7200

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     7260

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     7320

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     7380

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     7440

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     7500

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata     7560

cgcaaaccgc ctctccccgc gcgttggccg attcattaat g                         7601


<210>  49
<211>  1325
<212>  DNA
<213>  Homo sapiens

<400>  49
agtccagggc tctgtcctgc acctggggaa tggtgaccgg catctctgtc ctctagctct       60

ggaagcaccc cagcccctct agtctgccct cacccctgac cctgaccctc caccctgacc      120

ccgtcctaac ccctgacctt tgtgcccttc cagagagaag ggcagaagtg cccacagccc      180

accccagccc ctcacccagg ccagccggcc agttccaaac cctggtggtt ggtgtcgtgg      240

gcggcctgct gggcagcctg gtgctgctag tctgggtcct ggccgtcatc tgctcccggg      300

ccgcacgagg taacgtcatc ccagcccctc ggcctgccct gccctaaccc tgctggcggc      360

cctcactccc gcctcccctt cctccaccct tccctcaccc caccccacct ccccccatct      420

ccccgccagg ctaagtccct gatgaaggcc cctggactaa gaccccccac ctaggagcac      480

ggctcagggt cggcctggtg accccaagtg tgtttctctg cagggacaat aggagccagg      540

cgcaccggcc agcccctggt gagtctcact cttttcctgc atgatccact gtgccttcct      600

tcctgggtgg gcagaggtgg aaggacaggc tgggaccaca cggcctgcag gactcacatt      660

ctattatagc caggacccca cctccccagc ccccaggcag caacctcaat ccctaaagcc      720

atgatctggg gccccagccc acctgcggtc tccgggggtg cccggcccat gtgtgtgcct      780

gcctgcggtc tccaggggtg cctggcccac gcgtgtgccc gcctgcggtc tctgggggtg      840

cccggcccac atatgtgcct gcctgcggtc tccaggtgtg cccggcccat gcgtgtgccc      900

acctgcgagg gcgtggggtg ggcttggtca tttcttatct tacattggag acaggagagc      960

ttgaaaagtc acattttgga atcctaaatc tgcaagaatg ccagggacat ttcagagggg     1020

gacattgagc cagagaggag gggtggtgtc cccagatcac acagagggca gtggtgggac     1080

agctcagggt aagcagctcg tagtgggggg cccaggttcg gtgccggtac tgcagccagg     1140

ctgtggagcc gcgggcctcc ttcctgcggt gggccgtggg gctgactccc tctccctttc     1200

tcctcaaaga aggaggaccc ctcagccgtg cctgtgttct ctgtggacta tggggagctg     1260

gatttccagt ggcgagagaa gaccccggag ccccccgtgc cctgtgtccc tgagcagacg     1320

gagta                                                                 1325


<210>  50
<211>  1072
<212>  DNA
<213>  Homo sapiens

<400>  50
ccaccattgt ctttcctagc ggaatgggca cctcatcccc cgcccgcagg ggctcagccg       60

acggccctcg gagtgcccag ccactgaggc ctgaggatgg acactgctct tggcccctct      120

gaccggcttc cttggccacc agtgttctgc agaccctcca ccatgagccc gggtcagcgc      180

atttcctcag gagaagcagg cagggtgcag gccattgcag gccgtccagg ggctgagctg      240

cctgggggcg accggggctc cagcctgcac ctgcaccagg cacagcccca ccacaggact      300

catgtctcaa tgcccacagt gagcccaggc agcaggtgtc accgtcccct acagggaggg      360

ccagatgcag tcactgcttc aggtcctgcc agcacagagc tgcctgcgtc cagctccctg      420

aatctctgct gctgctgctg ctgctgctgc tgctgcctgc ggcccggggc tgaaggcgcc      480

gtggccctgc ctgacgcccc ggagcctcct gcctgaactt gggggctggt tggagatggc      540

cttggagcag ccaaggtgcc cctggcagtg gcatcccgaa acgccctgga cgcagggccc      600

aagactgggc acaggagtgg gaggtacatg gggctgggga ctccccagga gttatctgct      660

ccctgcaggc ctagagaagt ttcagggaag gtcagaagag ctcctggctg tggtgggcag      720

ggcaggaaac ccctccacct ttacacatgc ccaggcagca cctcaggccc tttgtggggc      780

agggaagctg aggcagtaag cgggcaggca gagctggagg cctttcaggc ccagccagca      840

ctctggcctc ctgccgccgc attccacccc agcccctcac accactcggg agagggacat      900

cctacggtcc caaggtcagg agggcagggc tggggttgac tcaggcccct cccagctgtg      960

gccacctggg tgttgggagg gcagaagtgc aggcacctag ggccccccat gtgcccaccc     1020

tgggagctct ccttggaacc cattcctgaa attatttaaa ggggttggcc gg             1072


<210>  51
<211>  7880
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized rAAV PD-1 Exon5_MND-PD-1-CD28 Switch Receptor 
       construct

<400>  51
cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc       60

tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc      120

actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagaattca      180

gtccagggct ctgtcctgca cctggggaat ggtgaccggc atctctgtcc tctagctctg      240

gaagcacccc agcccctcta gtctgccctc acccctgacc ctgaccctcc accctgaccc      300

cgtcctaacc cctgaccttt gtgcccttcc agagagaagg gcagaagtgc ccacagccca      360

ccccagcccc tcacccaggc cagccggcca gttccaaacc ctggtggttg gtgtcgtggg      420

cggcctgctg ggcagcctgg tgctgctagt ctgggtcctg gccgtcatct gctcccgggc      480

cgcacgaggt aacgtcatcc cagcccctcg gcctgccctg ccctaaccct gctggcggcc      540

ctcactcccg cctccccttc ctccaccctt ccctcacccc accccacctc cccccatctc      600

cccgccaggc taagtccctg atgaaggccc ctggactaag accccccacc taggagcacg      660

gctcagggtc ggcctggtga ccccaagtgt gtttctctgc agggacaata ggagccaggc      720

gcaccggcca gcccctggtg agtctcactc ttttcctgca tgatccactg tgccttcctt      780

cctgggtggg cagaggtgga aggacaggct gggaccacac ggcctgcagg actcacattc      840

tattatagcc aggaccccac ctccccagcc cccaggcagc aacctcaatc cctaaagcca      900

tgatctgggg ccccagccca cctgcggtct ccgggggtgc ccggcccatg tgtgtgcctg      960

cctgcggtct ccaggggtgc ctggcccacg cgtgtgcccg cctgcggtct ctgggggtgc     1020

ccggcccaca tatgtgcctg cctgcggtct ccaggtgtgc ccggcccatg cgtgtgccca     1080

cctgcgaggg cgtggggtgg gcttggtcat ttcttatctt acattggaga caggagagct     1140

tgaaaagtca cattttggaa tcctaaatct gcaagaatgc cagggacatt tcagaggggg     1200

acattgagcc agagaggagg ggtggtgtcc ccagatcaca cagagggcag tggtgggaca     1260

gctcagggta agcagctcat agtggggggc ccaggttcgg tgccggtact gcagccaggc     1320

tgtggagccg cgggcctcct tcctgcggtg ggccgtgggg ctgactccct ctccctttct     1380

cctcaaagaa ggaggacccc tcagccgtgc ctgtgttctc tgtggactat ggggagctgg     1440

atttccagtg gcgagagaag accccggagc cccccgtgcc ctgtgtccct gagcagacgg     1500

agtaatgcat gaacagagaa acaggagaat atgggccaaa caggatatct gtggtaagca     1560

gttcctgccc cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga     1620

tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc     1680

ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc     1740

tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc     1800

gcgcttctgc tccccgagct ctatataagc agagctcgtt tagtgaaccg tcagatcgcc     1860

tggagacgcc atccacgctg ttttgacttc catagaagga tctcgaggcc accatgcaga     1920

tcccgcaagc gccctggcca gtcgtctggg cggtgctaca actgggctgg cggccaggat     1980

ggttcttaga ctccccagac aggccctgga acccccccac cttctcccca gccctgctcg     2040

tggtgaccga aggggacaac gccaccttca cctgcagctt ctccaacaca tcggagagct     2100

tcgtgctaaa ctggtaccgc atgagcccca gcaaccagac ggacaagctg gccgccttcc     2160

ccgaggaccg cagccagccc ggccaggact gccgcttccg tgtcacacaa ctgcccaacg     2220

ggcgtgactt ccacatgagc gtggtcaggg cccggcgcaa tgacagcggc acctacctct     2280

gtggggccat ctccctggcc cccaaggcgc agatcaaaga gagcctgcgg gcagagctca     2340

gggtgacaga gagaagggca gaagtgccca cagcccaccc cagcccctca cccaggccag     2400

ccggccagtt ccaaaccctg gtggttggtg tcgtgggcgg cctgctgggc agcctggtgc     2460

tgctagtctg ggtcctggcc gtcatcagga gtaagaggag caggctcctg cacagtgact     2520

acatgaacat gactccccgc cgccccgggc ccacccgcaa gcattaccag ccctatgccc     2580

caccacgcga cttcgcagcc tatcgctccg gtgagggcag aggaagtctt ctaacatgcg     2640

gtgacgtgga ggagaatccg ggccctgtga gcaagggcga ggaggataac tccgccatca     2700

tcaaggagtt cctgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg     2760

agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg     2820

tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg     2880

gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc     2940

ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga     3000

cccaggactc ctctctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca     3060

acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg     3120

agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga     3180

aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc     3240

agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact     3300

acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg     3360

agctgtacaa gtgatgaaag ctttgcttta tttgtgaaat ttgtgatgct attgctttat     3420

ttgtaaccat tataagctgc aataaacaag tttaacaaca acaattgcat tcattttatg     3480

tttcaggttc agggggaggt gtgggaggtt ttttaaagtc gacgtggccc tgcctgacgc     3540

cccggagcct cctgcctgaa cttgggggct ggttggagat ggccttggag cagccaaggt     3600

gcccctggca gtggcatccc gaaacgccct ggacgcaggg cccaagactg ggcacaggag     3660

tgggaggtac atggggctgg ggactcccca ggagttatct gctccctgca ggcctagaga     3720

agtttcaggg aaggtcagaa gagctcctgg ctgtggtggg cagggcagga aacccctcca     3780

cctttacaca tgcccaggca gcacctcagg ccctttgtgg ggcagggaag ctgaggcagt     3840

aagcgggcag gcagagctgg aggcctttca ggcccagcca gcactctggc ctcctgccgc     3900

cgcattccac cccagcccct cacaccactc gggagaggga catcctacgg tcccaaggtc     3960

aggagggcag ggctggggtt gactcaggcc cctcccagct gtggccacct gggtgttggg     4020

agggcagaag tgcaggcacc tagggccccc catgtgccca ccctgggagc tctccttgga     4080

acccattcct gaaattattt aaaggggttg gccggactag ttacgtagat aagtagcatg     4140

gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact ccctctctgc     4200

gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc     4260

gggcggcctc agtgagcgag cgagcgcgcc agctggcgta atagcgaaga ggcccgcacc     4320

gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgattccg ttgcaatggc     4380

tggcggtaat attgttctgg atattaccag caaggccgat agtttgagtt cttctactca     4440

ggcaagtgat gttattacta atcaaagaag tattgcgaca acggttaatt tgcgtgatgg     4500

acagactctt ttactcggtg gcctcactga ttataaaaac acttctcagg attctggcgt     4560

accgttcctg tctaaaatcc ctttaatcgg cctcctgttt agctcccgct ctgattctaa     4620

cgaggaaagc acgttatacg tgctcgtcaa agcaaccata gtacgcgccc tgtagcggcg     4680

cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc     4740

tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc     4800

gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg     4860

accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg     4920

tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg     4980

gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt     5040

cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa     5100

tattaacgtt tacaatttaa atatttgctt atacaatctt cctgtttttg gggcttttct     5160

gattatcaac cggggtacat atgattgaca tgctagtttt acgattaccg ttcatcgatt     5220

ctcttgtttg ctccagactc tcaggcaatg acctgatagc ctttgtagag acctctcaaa     5280

aatagctacc ctctccggca tgaatttatc agctagaacg gttgaatatc atattgatgg     5340

tgatttgact gtctccggcc tttctcaccc gtttgaatct ttacctacac attactcagg     5400

cattgcattt aaaatatatg agggttctaa aaatttttat ccttgcgttg aaataaaggc     5460

ttctcccgca aaagtattac agggtcataa tgtttttggt acaaccgatt tagctttatg     5520

ctctgaggct ttattgctta attttgctaa ttctttgcct tgcctgtatg atttattgga     5580

tgttggaatc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc     5640

atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac     5700

ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga     5760

caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa     5820

cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata     5880

atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt     5940

ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg     6000

cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt     6060

cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta     6120

aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc     6180

ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa     6240

gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc     6300

cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt     6360

acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact     6420

gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac     6480

aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata     6540

ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta     6600

ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg     6660

gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat     6720

aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt     6780

aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga     6840

aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa     6900

gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag     6960

gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac     7020

tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc     7080

gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat     7140

caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat     7200

actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct     7260

acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt     7320

cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg     7380

gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta     7440

cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg     7500

gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg     7560

tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc     7620

tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg     7680

gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat     7740

aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc     7800

agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg     7860

cgttggccga ttcattaatg                                                 7880


<210>  52
<211>  7593
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized rAAV PD-1 Exon5_MND-BFP_distal 5'-Homology Arm 
       construct

<400>  52
cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc       60

tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc      120

actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagaattcg      180

gccccactgc ccactgccca gggcagcaat gcccatacca cgtggtccca gctccgagct      240

tgtcctgaaa agggggcaaa gactggaccc tgagcctgcc aaggggccac actcctccca      300

gggctggggt ctccatgggc agccccccac ccacccagac cagttacact cccctgtgcc      360

agagcagtgc agacaggacc aggccaggat gcccaagggt caggggctgg ggatgggtag      420

cccccaaaca gccctttctg ggggactggc ctcaacgggg aagggggtga aggctcttag      480

taggaaatca gggagaccca agtcagagcc aggcgctgtg cagaagctgc agcctcacgt      540

agaaggaaga gcctctgcag tggaggccag tgcccatccc cgggtggcag aggccccagc      600

agagacttct caatgacatt ccagctgggg tggcccttcc agagcccttg ctgcccgagg      660

gatgtgagca ggtggccggg gaggctttgt ggggccaccc agccccttcc tcacctctct      720

ccatctctca gactccccag acaggccctg gaaccccccc accttctccc cagccctgct      780

cgtggtgacc gaaggggaca acgccacctt cacctgcagc ttctccaaca catcggagag      840

cttcgtgcta aactggtacc gcatgagccc cagcaaccag acggacaagc tggccgcctt      900

ccccgaggac cgcagccagc ccggccagga ctgccgcttc cgtgtcacac aactgcccaa      960

cgggcgtgac ttccacatga gcgtggtcag ggcccggcgc aatgacagcg gcacctacct     1020

ctgtggggcc atctccctgg cccccaaggc gcagatcaaa gagagcctgc gggcagagct     1080

cagggtgaca ggtgcggcct cggaggcccc ggggcagggg tgagctgagc cggtcctggg     1140

gtgggtgtcc cctcctgcac aggatcagga gctccagggt cgtagggcag ggacccccca     1200

gctccagtcc agggctctgt cctgcacctg gggaatggtg accggcatct ctgtcctcta     1260

gctctggaag caccccagcc cctctagtct gccctcaccc ctgaccctga ccctccaccc     1320

tgaccccgtc ctaacccctg acctttgtgc ccttccagag agaagggcag aagtgcccac     1380

agcccacccc agcccctcac ccaggccagc cggccagttc caaaccctgg tggttggtgt     1440

cgtgggcggc ctgctgggca gcctggtgct gctagtctgg gtcctggccg tcatctatgc     1500

atgaacagag aaacaggaga atatgggcca aacaggatat ctgtggtaag cagttcctgc     1560

cccggctcag ggccaagaac agttggaaca gcagaatatg ggccaaacag gatatctgtg     1620

gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc     1680

cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga     1740

ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct     1800

gctccccgag ctctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg     1860

ccatccacgc tgttttgact tccatagaag gatctcgagg ccaccatggc tagcgagctg     1920

attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc     1980

aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag     2040

gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac     2100

ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc     2160

cctgagggct tcacatggga gagagtcacc acatacgagg acgggggcgt gctgaccgct     2220

acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg     2280

aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc     2340

gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc     2400

gtgggcggga gccatctgat cgcaaacatc aagaccacat atagatccaa gaaacccgct     2460

aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag     2520

gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc     2580

cctagcaaac tggggcacaa gctaaattga aagctttgct ttatttgtga aatttgtgat     2640

gctattgctt tatttgtaac cattataagc tgcaataaac aagtttaaca acaacaattg     2700

cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gtcgacccac     2760

cattgtcttt cctagcggaa tgggcacctc atcccccgcc cgcaggggct cagctgacgg     2820

ccctcggagt gcccagccac tgaggcctga ggatggacac tgctcttggc ccctctgacc     2880

ggcttccttg gccaccagtg ttctgcagac cctccaccat gagcccgggt cagcgcattt     2940

cctcaggaga agcaggcagg gtgcaggcca ttgcaggccg tccaggggct gagctgcctg     3000

ggggcgaccg gggctccagc ctgcacctgc accaggcaca gccccaccac aggactcatg     3060

tctcaatgcc cacagtgagc ccaggcagca ggtgtcaccg tcccctacag ggagggccag     3120

atgcagtcac tgcttcaggt cctgccagca cagagctgcc tgcgtccagc tccctgaatc     3180

tctgctgctg ctgctgctgc tgctgctgct gcctgcggcc cggggctgaa ggcgccgtgg     3240

ccctgcctga cgccccggag cctcctgcct gaacttgggg gctggttgga gatggccttg     3300

gagcagccaa ggtgcccctg gcagtggcat cccgaaacgc cctggacgca gggcccaaga     3360

ctgggcacag gagtgggagg tacatggggc tggggactcc ccaggagtta tctgctccct     3420

gcaggcctag agaagtttca gggaaggtca gaagagctcc tggctgtggt gggcagggca     3480

ggaaacccct ccacctttac acatgcccag gcagcacctc aggccctttg tggggcaggg     3540

aagctgaggc agtaagcggg caggcagagc tggaggcctt tcaggcccag ccagcactct     3600

ggcctcctgc cgccgcattc caccccagcc cctcacacca ctcgggagag ggacatccta     3660

cggtcccaag gtcaggaggg cagggctggg gttgactcag gcccctccca gctgtggcca     3720

cctgggtgtt gggagggcag aagtgcaggc acctagggcc ccccatgtgc ccaccctggg     3780

agctctcctt ggaacccatt cctgaaatta tttaaagggg ttggccggac tagttacgta     3840

gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc     3900

actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc     3960

ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gccagctggc gtaatagcga     4020

agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgatt     4080

ccgttgcaat ggctggcggt aatattgttc tggatattac cagcaaggcc gatagtttga     4140

gttcttctac tcaggcaagt gatgttatta ctaatcaaag aagtattgcg acaacggtta     4200

atttgcgtga tggacagact cttttactcg gtggcctcac tgattataaa aacacttctc     4260

aggattctgg cgtaccgttc ctgtctaaaa tccctttaat cggcctcctg tttagctccc     4320

gctctgattc taacgaggaa agcacgttat acgtgctcgt caaagcaacc atagtacgcg     4380

ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca     4440

cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc     4500

gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct     4560

ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg     4620

ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc     4680

ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg     4740

attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg     4800

aattttaaca aaatattaac gtttacaatt taaatatttg cttatacaat cttcctgttt     4860

ttggggcttt tctgattatc aaccggggta catatgattg acatgctagt tttacgatta     4920

ccgttcatcg attctcttgt ttgctccaga ctctcaggca atgacctgat agcctttgta     4980

gagacctctc aaaaatagct accctctccg gcatgaattt atcagctaga acggttgaat     5040

atcatattga tggtgatttg actgtctccg gcctttctca cccgtttgaa tctttaccta     5100

cacattactc aggcattgca tttaaaatat atgagggttc taaaaatttt tatccttgcg     5160

ttgaaataaa ggcttctccc gcaaaagtat tacagggtca taatgttttt ggtacaaccg     5220

atttagcttt atgctctgag gctttattgc ttaattttgc taattctttg ccttgcctgt     5280

atgatttatt ggatgttgga atcgcctgat gcggtatttt ctccttacgc atctgtgcgg     5340

tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag     5400

ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc     5460

atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc     5520

gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa     5580

tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg     5640

aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata     5700

accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg     5760

tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac     5820

gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact     5880

ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat     5940

gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga     6000

gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac     6060

agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat     6120

gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac     6180

cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct     6240

gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac     6300

gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga     6360

ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg     6420

gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact     6480

ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac     6540

tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta     6600

actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt     6660

taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga     6720

gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc     6780

tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt     6840

ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc     6900

gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc     6960

tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg     7020

cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg     7080

gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga     7140

actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc     7200

ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg     7260

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg     7320

atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt     7380

tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc     7440

tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg     7500

aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc     7560

gcctctcccc gcgcgttggc cgattcatta atg                                  7593


<210>  53
<211>  6718
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized rAAV PD-1 Exon1_MND-GFP construct

<400>  53
cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc       60

tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc      120

actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtaaggctgt      180

tgcaggcatc acacggtgga aagatctgga actgtggcca tggtgtgagg ccatccacaa      240

ggtggaagct ttgaggggga gccgattagc catggacagt tgtcattcag tagggtcacc      300

tgtgccccag cgaaggggga tgggccggga aggcagaggc caggcacctg cccccagcag      360

gggcagaggc tgtgggcagc cgggaggctc ccagaggctc cgacagaatg ggagtggggt      420

tgagcccacc cctcactgca gcccaggaac ctgagcccag agggggccac ccaccttccc      480

caggcaggga ggcccggccc ccagggagat gggggggatg ggggaggaga agggcctgcc      540

cccacccggc agcctcagga ggggcagctc gggcgggata tggaaagagg ccacagcagt      600

gagcagagac acagaggagg aaggggccct gagctgggga gacccccacg gggtagggcg      660

tgggggccac gggcccacct cctccccatc tcctctgtct ccctgtctct gtctctctct      720

ccctccccca ccctctcccc agtcctaccc cctcctcacc cctcctcccc cagcactgcc      780

tctgtcactc tcgcccacgt ggatgtggag gaagaggggg cgggagcaag gggcgggcac      840

cctcccttca acctgacctg ggacagtttc ccttccgctc acctccgcct gagcagtgga      900

gaaggcggca ctctggtggg gctgctccaa cgcgtgaaca gagaaacagg agaatatggg      960

ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagttgga     1020

acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg     1080

ccaagaacag atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag     1140

atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat     1200

cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctctata taagcagagc     1260

tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acttccatag     1320

aaggatctcg aggccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc     1380

atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc     1440

gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg     1500

cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc     1560

taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc     1620

caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag     1680

ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac     1740

ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg     1800

gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac     1860

ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg     1920

ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag     1980

aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg     2040

gacgagctgt acaagtaagc ggccgcgctt tatttgtgaa atttgtgatg ctattgcttt     2100

atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat     2160

gtttcaggtt cagggggaga tgtgggaggt tttttaaagc ctcaccggtt ctgggcggtg     2220

ctacaactgg gctggcggcc aggatggttc ttaggtaggt ggggtcggcg gtcaggtgtc     2280

ccagagccag gggtctggag ggaccttcca ccctcagtcc ctggcaggtc ggggggtgct     2340

gaggcgggcc tggccctggc agcccagggg tcccggagcg aggggtctgg agggaccttt     2400

cactctcagt ccctggcagg tcggggggtg ctgtggcagg cccagccttg gcccccagct     2460

ctgcccctta ccctgagctg tgtggctttg ggcagctcga actcctgggt tcctctctgg     2520

gccccaactc ctcccctggc ccaagtcccc tctttgctcc tgggcaggca ggacctctgt     2580

cccctctcag ccggtccttg gggctgcgtg tttctgtaga atgacgggtc aggctggcca     2640

gaaccccaaa ccttggccgt ggggagtctg cgtggcggct ctgccttgcc caggcatcct     2700

tggtcctcac tcgagttttc ctaaggatgg gatgagcccc atgtgggact aaccttggct     2760

ttacgacgtc aaagtttaga tgagctggtg atatttttct cattatatcc aaagtgtacc     2820

tgttcgagtg aggacagttc ttctgtctcc aggatccctc ctgggtgggg attgtgcccg     2880

cctgggtctc tgcccagatt ccagggctct ccccgagccc tgttcagacc atccgtgggg     2940

gaggccttgg cctcactctt acgtagataa gtagcatggc gggttaatca ttaactacaa     3000

ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc     3060

cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg     3120

agcgcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc     3180

gcagcctgaa tggcgaatgg cgattccgtt gcaatggctg gcggtaatat tgttctggat     3240

attaccagca aggccgatag tttgagttct tctactcagg caagtgatgt tattactaat     3300

caaagaagta ttgcgacaac ggttaatttg cgtgatggac agactctttt actcggtggc     3360

ctcactgatt ataaaaacac ttctcaggat tctggcgtac cgttcctgtc taaaatccct     3420

ttaatcggcc tcctgtttag ctcccgctct gattctaacg aggaaagcac gttatacgtg     3480

ctcgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt     3540

ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt     3600

cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct     3660

ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg     3720

tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga     3780

gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc     3840

ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga     3900

gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttaaat     3960

atttgcttat acaatcttcc tgtttttggg gcttttctga ttatcaaccg gggtacatat     4020

gattgacatg ctagttttac gattaccgtt catcgattct cttgtttgct ccagactctc     4080

aggcaatgac ctgatagcct ttgtagagac ctctcaaaaa tagctaccct ctccggcatg     4140

aatttatcag ctagaacggt tgaatatcat attgatggtg atttgactgt ctccggcctt     4200

tctcacccgt ttgaatcttt acctacacat tactcaggca ttgcatttaa aatatatgag     4260

ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag     4320

ggtcataatg tttttggtac aaccgattta gctttatgct ctgaggcttt attgcttaat     4380

tttgctaatt ctttgccttg cctgtatgat ttattggatg ttggaatcgc ctgatgcggt     4440

attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa     4500

tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc     4560

cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga     4620

gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg     4680

tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg     4740

gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa     4800

atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga     4860

agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc     4920

ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg     4980

gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc     5040

gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat     5100

tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg     5160

acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag     5220

aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa     5280

cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc     5340

gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca     5400

cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc     5460

tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc     5520

tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg     5580

ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta     5640

tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag     5700

gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga     5760

ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc     5820

tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa     5880

agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa     5940

aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc     6000

cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt     6060

agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc     6120

tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac     6180

gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca     6240

gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg     6300

ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag     6360

gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt     6420

ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat     6480

ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc     6540

acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt     6600

gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag     6660

cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatg       6718


<210>  54
<211>  756
<212>  DNA
<213>  Homo sapiens

<400>  54
aggctgttgc aggcatcaca cggtggaaag atctggaact gtggccatgg tgtgaggcca       60

tccacaaggt ggaagctttg agggggagcc gattagccat ggacagttgt cattcagtag      120

ggtcacctgt gccccagcga agggggatgg gccgggaagg cagaggccag gcacctgccc      180

ccagcagggg cagaggctgt gggcagccgg gaggctccca gaggctccga cagaatggga      240

gtggggttga gcccacccct cactgcagcc caggaacctg agcccagagg gggccaccca      300

ccttccccag gcagggaggc ccggccccca gggagatggg ggggatgggg gaggagaagg      360

gcctgccccc acccggcagc ctcaggaggg gcagctcggg cgggatatgg aaagaggcca      420

cagcagtgag cagagacaca gaggaggaag gggccctgag ctggggagac ccccacgggg      480

tagggcgtgg gggccacggg cccacctcct ccccatctcc tctgtctccc tgtctctgtc      540

tctctctccc tcccccaccc tctccccagt cctaccccct cctcacccct cctcccccag      600

cactgcctct gtcactctcg cccacgtgga tgtggaggaa gagggggcgg gagcaagggg      660

cgggcaccct cccttcaacc tgacctggga cagtttccct tccgctcacc tccgcctgag      720

cagtggagaa ggcggcactc tggtggggct gctcca                                756


<210>  55
<211>  750
<212>  DNA
<213>  Homo sapiens

<400>  55
tctgggcggt gctacaactg ggctggcggc caggatggtt cttaggtagg tggggtcggc       60

ggtcaggtgt cccagagcca ggggtctgga gggaccttcc accctcagtc cctggcaggt      120

cggggggtgc tgaggcgggc ctggccctgg cagcccaggg gtcccggagc gaggggtctg      180

gagggacctt tcactctcag tccctggcag gtcggggggt gctgtggcag gcccagcctt      240

ggcccccagc tctgcccctt accctgagct gtgtggcttt gggcagctcg aactcctggg      300

ttcctctctg ggccccaact cctcccctgg cccaagtccc ctctttgctc ctgggcaggc      360

aggacctctg tcccctctca gccggtcctt ggggctgcgt gtttctgtag aatgacgggt      420

caggctggcc agaaccccaa accttggccg tggggagtct gcgtggcggc tctgccttgc      480

ccaggcatcc ttggtcctca ctcgagtttt cctaaggatg ggatgagccc catgtgggac      540

taaccttggc tttacgacgt caaagtttag atgagctggt gatatttttc tcattatatc      600

caaagtgtac ctgttcgagt gaggacagtt cttctgtctc caggatccct cctgggtggg      660

gattgtgccc gcctgggtct ctgcccagat tccagggctc tccccgagcc ctgttcagac      720

catccgtggg ggaggccttg gcctcactct                                       750


<210>  56
<211>  7609
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized rAAV PD-1 Exon1_MND-CD19CAR construct

<400>  56
cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc       60

tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc      120

actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtaaggctgt      180

tgcaggcatc acacggtgga aagatctgga actgtggcca tggtgtgagg ccatccacaa      240

ggtggaagct ttgaggggga gccgattagc catggacagt tgtcattcag tagggtcacc      300

tgtgccccag cgaaggggga tgggccggga aggcagaggc caggcacctg cccccagcag      360

gggcagaggc tgtgggcagc cgggaggctc ccagaggctc cgacagaatg ggagtggggt      420

tgagcccacc cctcactgca gcccaggaac ctgagcccag agggggccac ccaccttccc      480

caggcaggga ggcccggccc ccagggagat gggggggatg ggggaggaga agggcctgcc      540

cccacccggc agcctcagga ggggcagctc gggcgggata tggaaagagg ccacagcagt      600

gagcagagac acagaggagg aaggggccct gagctgggga gacccccacg gggtagggcg      660

tgggggccac gggcccacct cctccccatc tcctctgtct ccctgtctct gtctctctct      720

ccctccccca ccctctcccc agtcctaccc cctcctcacc cctcctcccc cagcactgcc      780

tctgtcactc tcgcccacgt ggatgtggag gaagaggggg cgggagcaag gggcgggcac      840

cctcccttca acctgacctg ggacagtttc ccttccgctc acctccgcct gagcagtgga      900

gaaggcggca ctctggtggg gctgctccaa cgcgtgatcc atcgattagt ccaatttgtt      960

aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag     1020

cctatagagt acgagccata gatagaataa aagattttat ttagtctcca gaaaaagggg     1080

ggaatgaaag accccacctg taggtttggc aagctaggat caaggttagg aacagagaga     1140

cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc     1200

caagaacagt tggaacagca gaatatgggc caaacaggat atctgtggta agcagttcct     1260

gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc     1320

tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat     1380

ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc     1440

aataaaagag cccacaaccc ctcactcggc gcgacgcgtc atagccacca tggccttacc     1500

agtgaccgcc ttgctcctgc cgctggcctt gctgctccac gccgccaggc cggacatcca     1560

gatgacacag actacatcct ccctgtctgc ctctctggga gacagagtca ccatcagttg     1620

cagggcaagt caggacatta gtaaatattt aaattggtat cagcagaaac cagatggaac     1680

tgttaaactc ctgatctacc atacatcaag attacactca ggagtcccat caaggttcag     1740

tggcagtggg tctggaacag attattctct caccattagc aacctggagc aagaagatat     1800

tgccacttac ttttgccaac agggtaatac gcttccgtac acgttcggag gggggaccaa     1860

gctggagatc acaggtggcg gtggctccgg cggtggtggg tctggtggcg gcggaagcga     1920

ggtgaaactg caggagtcag gacctggcct ggtggcgccc tcacagagcc tgtccgtcac     1980

atgcactgtc tcaggggtct cattacccga ctatggtgta agctggattc gccagcctcc     2040

acgaaagggt ctggagtggc tgggagtaat atggggtagt gaaaccacat actataattc     2100

agctctcaaa tccagactga ccatcatcaa ggacaactcc aagagccaag ttttcttaaa     2160

aatgaacagt ctgcaaactg atgacacagc catttactac tgtgccaaac attattacta     2220

cggtggtagc tatgctatgg actactgggg tcaaggaacc tcggtcaccg tctcctcaac     2280

cacgacgcca gcgccgcgac caccaacacc ggcgcccacc atcgcgtcgc agcccctgtc     2340

cctgcgccca gaggcgtgcc ggccagcggc ggggggcgca gtgcacacga gggggctgga     2400

cttcgcctgt gatatctaca tctgggcgcc cttggccggg acttgtgggg tccttctcct     2460

gtcactggtg atcacccttt actgcaaacg gggcagaaag aaactcctgt atatattcaa     2520

acaaccattt atgagaccag tacaaactac tcaagaggaa gatggctgta gctgccgatt     2580

tccagaagaa gaagaaggag gatgtgaact gagagtgaag ttcagcagga gcgcagacgc     2640

ccccgcgtac cagcagggcc agaaccagct ctataacgag ctcaatctag gacgaagaga     2700

ggagtacgat gttttggaca agagacgtgg ccgggaccct gagatggggg gaaagccgag     2760

aaggaagaac cctcaggaag gcctgtacaa tgaactgcag aaagataaga tggcggaggc     2820

ctacagtgag attgggatga aaggcgagcg ccggaggggc aaggggcacg atggccttta     2880

ccagggtctc agtacagcca ccaaggacac ctacgacgcc cttcacatgc aggccctgcc     2940

ccctcgctaa gcggccgcgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa     3000

ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg     3060

ttcaggggga gatgtgggag gttttttaaa gctcaccggt tctgggcggt gctacaactg     3120

ggctggcggc caggatggtt cttaggtagg tggggtcggc ggtcaggtgt cccagagcca     3180

ggggtctgga gggaccttcc accctcagtc cctggcaggt cggggggtgc tgaggcgggc     3240

ctggccctgg cagcccaggg gtcccggagc gaggggtctg gagggacctt tcactctcag     3300

tccctggcag gtcggggggt gctgtggcag gcccagcctt ggcccccagc tctgcccctt     3360

accctgagct gtgtggcttt gggcagctcg aactcctggg ttcctctctg ggccccaact     3420

cctcccctgg cccaagtccc ctctttgctc ctgggcaggc aggacctctg tcccctctca     3480

gccggtcctt ggggctgcgt gtttctgtag aatgacgggt caggctggcc agaaccccaa     3540

accttggccg tggggagtct gcgtggcggc tctgccttgc ccaggcatcc ttggtcctca     3600

ctcgagtttt cctaaggatg ggatgagccc catgtgggac taaccttggc tttacgacgt     3660

caaagtttag atgagctggt gatatttttc tcattatatc caaagtgtac ctgttcgagt     3720

gaggacagtt cttctgtctc caggatccct cctgggtggg gattgtgccc gcctgggtct     3780

ctgcccagat tccagggctc tccccgagcc ctgttcagac catccgtggg ggaggccttg     3840

gcctcactct tacgtagata agtagcatgg cgggttaatc attaactaca aggaacccct     3900

agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc     3960

aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcca     4020

gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga     4080

atggcgaatg gcgattccgt tgcaatggct ggcggtaata ttgttctgga tattaccagc     4140

aaggccgata gtttgagttc ttctactcag gcaagtgatg ttattactaa tcaaagaagt     4200

attgcgacaa cggttaattt gcgtgatgga cagactcttt tactcggtgg cctcactgat     4260

tataaaaaca cttctcagga ttctggcgta ccgttcctgt ctaaaatccc tttaatcggc     4320

ctcctgttta gctcccgctc tgattctaac gaggaaagca cgttatacgt gctcgtcaaa     4380

gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg     4440

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc     4500

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg     4560

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc     4620

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt     4680

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc     4740

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta     4800

acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttaaa tatttgctta     4860

tacaatcttc ctgtttttgg ggcttttctg attatcaacc ggggtacata tgattgacat     4920

gctagtttta cgattaccgt tcatcgattc tcttgtttgc tccagactct caggcaatga     4980

cctgatagcc tttgtagaga cctctcaaaa atagctaccc tctccggcat gaatttatca     5040

gctagaacgg ttgaatatca tattgatggt gatttgactg tctccggcct ttctcacccg     5100

tttgaatctt tacctacaca ttactcaggc attgcattta aaatatatga gggttctaaa     5160

aatttttatc cttgcgttga aataaaggct tctcccgcaa aagtattaca gggtcataat     5220

gtttttggta caaccgattt agctttatgc tctgaggctt tattgcttaa ttttgctaat     5280

tctttgcctt gcctgtatga tttattggat gttggaatcg cctgatgcgg tattttctcc     5340

ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg     5400

atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg     5460

cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt     5520

gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc     5580

tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc     5640

ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc     5700

cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga     5760

gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt     5820

ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag     5880

tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag     5940

aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta     6000

ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg     6060

agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca     6120

gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag     6180

gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc     6240

gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg     6300

tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc     6360

ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg     6420

cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg     6480

gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga     6540

cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac     6600

tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa     6660

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca     6720

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag     6780

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac     6840

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa     6900

ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc     6960

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag     7020

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac     7080

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc     7140

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc     7200

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca     7260

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc     7320

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg     7380

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct     7440

ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata     7500

ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc     7560

gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatg                 7609


<210>  57
<211>  7492
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized rAAV PD-1 Exon1_MND-BCMACAR construct

<400>  57
cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc       60

tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc      120

actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtaaggctgt      180

tgcaggcatc acacggtgga aagatctgga actgtggcca tggtgtgagg ccatccacaa      240

ggtggaagct ttgaggggga gccgattagc catggacagt tgtcattcag tagggtcacc      300

tgtgccccag cgaaggggga tgggccggga aggcagaggc caggcacctg cccccagcag      360

gggcagaggc tgtgggcagc cgggaggctc ccagaggctc cgacagaatg ggagtggggt      420

tgagcccacc cctcactgca gcccaggaac ctgagcccag agggggccac ccaccttccc      480

caggcaggga ggcccggccc ccagggagat gggggggatg ggggaggaga agggcctgcc      540

cccacccggc agcctcagga ggggcagctc gggcgggata tggaaagagg ccacagcagt      600

gagcagagac acagaggagg aaggggccct gagctgggga gacccccacg gggtagggcg      660

tgggggccac gggcccacct cctccccatc tcctctgtct ccctgtctct gtctctctct      720

ccctccccca ccctctcccc agtcctaccc cctcctcacc cctcctcccc cagcactgcc      780

tctgtcactc tcgcccacgt ggatgtggag gaagaggggg cgggagcaag gggcgggcac      840

cctcccttca acctgacctg ggacagtttc ccttccgctc acctccgcct gagcagtgga      900

gaaggcggca ctctggtggg gctgctccaa cgcgtaatga aagaccccac ctgtaggttt      960

ggcaagctag gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat     1020

ctgtggtaag cagttcctgc cccggctcag ggccaagaac agttggaaca gcagaatatg     1080

ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg     1140

gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg tttccagggt     1200

gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc     1260

gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc     1320

ggcgcgattc acctgacgcg tctacgccac catggcactc cccgtcaccg cccttctctt     1380

gcccctcgcc ctgctgctgc atgctgccag gcccgacatt gtgctcactc agtcacctcc     1440

cagcctggcc atgagcctgg gaaaaagggc caccatctcc tgtagagcca gtgagtccgt     1500

cacaatcttg gggagccatc ttattcactg gtatcagcag aagcccgggc agcctccaac     1560

ccttcttatt cagctcgcgt caaacgtcca gacgggtgta cctgccagat tttctggtag     1620

cgggtcccgc actgatttta cactgaccat agatccagtg gaagaagacg atgtggccgt     1680

gtattattgt ctgcagagca gaacgattcc tcgcacattt ggtgggggta ctaagctgga     1740

gattaaggga agcacgtccg gctcagggaa gccgggctcc ggcgagggaa gcacgaaggg     1800

gcaaattcag ctggtccaga gcggacctga gctgaaaaaa cccggcgaga ctgttaagat     1860

cagttgtaaa gcatctggct ataccttcac cgactacagc ataaattggg tgaaacgggc     1920

ccctggaaag ggcctcaaat ggatgggttg gatcaatacc gaaactaggg agcctgctta     1980

tgcatatgac ttccgcggga gattcgcctt ttcactcgag acatctgcct ctactgctta     2040

cctccaaata aacaacctca agtatgaaga tacagccact tacttttgcg ccctcgacta     2100

tagttacgcc atggactact ggggacaggg aacctccgtt accgtcagtt ccgcggccgc     2160

aaccacaaca cctgctccaa ggccccccac acccgctcca actatagcca gccaaccatt     2220

gagcctcaga cctgaagctt gcaggcccgc agcaggaggc gccgtccata cgcgaggcct     2280

ggacttcgcg tgtgatattt atatttgggc ccctttggcc ggaacatgtg gggtgttgct     2340

tctctccctt gtgatcactc tgtattgtaa gcgcgggaga aagaagctcc tgtacatctt     2400

caagcagcct tttatgcgac ctgtgcaaac cactcaggaa gaagatgggt gttcatgccg     2460

cttccccgag gaggaagaag gagggtgtga actgagggtg aaattttcta gaagcgccga     2520

tgctcccgca tatcagcagg gtcagaatca gctctacaat gaattgaatc tcggcaggcg     2580

agaagagtac gatgttctgg acaagagacg gggcagggat cccgagatgg ggggaaagcc     2640

ccggagaaaa aatcctcagg aggggttgta caatgagctg cagaaggaca agatggctga     2700

agcctatagc gagatcggaa tgaaaggcga aagacgcaga ggcaaggggc atgacggtct     2760

gtaccagggt ctctctacag ccaccaagga cacttatgat gcgttgcata tgcaagcctt     2820

gccaccccgc taagcggccg cgctttattt gtgaaatttg tgatgctatt gctttatttg     2880

taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc     2940

aggttcaggg ggagatgtgg gaggtttttt aaagctcacc ggttctgggc ggtgctacaa     3000

ctgggctggc ggccaggatg gttcttaggt aggtggggtc ggcggtcagg tgtcccagag     3060

ccaggggtct ggagggacct tccaccctca gtccctggca ggtcgggggg tgctgaggcg     3120

ggcctggccc tggcagccca ggggtcccgg agcgaggggt ctggagggac ctttcactct     3180

cagtccctgg caggtcgggg ggtgctgtgg caggcccagc cttggccccc agctctgccc     3240

cttaccctga gctgtgtggc tttgggcagc tcgaactcct gggttcctct ctgggcccca     3300

actcctcccc tggcccaagt cccctctttg ctcctgggca ggcaggacct ctgtcccctc     3360

tcagccggtc cttggggctg cgtgtttctg tagaatgacg ggtcaggctg gccagaaccc     3420

caaaccttgg ccgtggggag tctgcgtggc ggctctgcct tgcccaggca tccttggtcc     3480

tcactcgagt tttcctaagg atgggatgag ccccatgtgg gactaacctt ggctttacga     3540

cgtcaaagtt tagatgagct ggtgatattt ttctcattat atccaaagtg tacctgttcg     3600

agtgaggaca gttcttctgt ctccaggatc cctcctgggt ggggattgtg cccgcctggg     3660

tctctgccca gattccaggg ctctccccga gccctgttca gaccatccgt gggggaggcc     3720

ttggcctcac tcttacgtag ataagtagca tggcgggtta atcattaact acaaggaacc     3780

cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg     3840

accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg     3900

ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc     3960

tgaatggcga atggcgattc cgttgcaatg gctggcggta atattgttct ggatattacc     4020

agcaaggccg atagtttgag ttcttctact caggcaagtg atgttattac taatcaaaga     4080

agtattgcga caacggttaa tttgcgtgat ggacagactc ttttactcgg tggcctcact     4140

gattataaaa acacttctca ggattctggc gtaccgttcc tgtctaaaat ccctttaatc     4200

ggcctcctgt ttagctcccg ctctgattct aacgaggaaa gcacgttata cgtgctcgtc     4260

aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac     4320

gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc     4380

ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt     4440

agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg     4500

ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac     4560

gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta     4620

ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat     4680

ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt aaatatttgc     4740

ttatacaatc ttcctgtttt tggggctttt ctgattatca accggggtac atatgattga     4800

catgctagtt ttacgattac cgttcatcga ttctcttgtt tgctccagac tctcaggcaa     4860

tgacctgata gcctttgtag agacctctca aaaatagcta ccctctccgg catgaattta     4920

tcagctagaa cggttgaata tcatattgat ggtgatttga ctgtctccgg cctttctcac     4980

ccgtttgaat ctttacctac acattactca ggcattgcat ttaaaatata tgagggttct     5040

aaaaattttt atccttgcgt tgaaataaag gcttctcccg caaaagtatt acagggtcat     5100

aatgtttttg gtacaaccga tttagcttta tgctctgagg ctttattgct taattttgct     5160

aattctttgc cttgcctgta tgatttattg gatgttggaa tcgcctgatg cggtattttc     5220

tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct     5280

ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac     5340

gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca     5400

tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac     5460

gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt     5520

ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt     5580

atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta     5640

tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg     5700

tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac     5760

gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg     5820

aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc     5880

gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg     5940

ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat     6000

gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg     6060

gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg     6120

atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc     6180

ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt     6240

cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct     6300

cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc     6360

gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca     6420

cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct     6480

cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt     6540

taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga     6600

ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca     6660

aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac     6720

caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg     6780

taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag     6840

gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac     6900

cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt     6960

taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg     7020

agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc     7080

ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc     7140

gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc     7200

acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa     7260

acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt     7320

tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg     7380

ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag     7440

agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tg             7492


<210>  58
<211>  6147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized rAAV PD-1 Exon1_ATG-mCherry construct

<400>  58
cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc       60

tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc      120

actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtaaggctgt      180

tgcaggcatc acacggtgga aagatctgga actgtggcca tggtgtgagg ccatccacaa      240

ggtggaagct ttgaggggga gccgattagc catggacagt tgtcattcag tagggtcacc      300

tgtgccccag cgaaggggga tgggccggga aggcagaggc caggcacctg cccccagcag      360

gggcagaggc tgtgggcagc cgggaggctc ccagaggctc cgacagaatg ggagtggggt      420

tgagcccacc cctcactgca gcccaggaac ctgagcccag agggggccac ccaccttccc      480

caggcaggga ggcccggccc ccagggagat gggggggatg ggggaggaga agggcctgcc      540

cccacccggc agcctcagga ggggcagctc gggcgggata tggaaagagg ccacagcagt      600

gagcagagac acagaggagg aaggggccct gagctgggga gacccccacg gggtagggcg      660

tgggggccac gggcccacct cctccccatc tcctctgtct ccctgtctct gtctctctct      720

ccctccccca ccctctcccc agtcctaccc cctcctcacc cctcctcccc cagcactgcc      780

tctgtcactc tcgcccacgt ggatgtggag gaagaggggg cgggagcaag gggcgggcac      840

cctcccttca acctgacctg ggacagtttc ccttccgctc acctccgcct gagcagtgga      900

gaaggcggca ctctggtggg gctgctccag gcatgcaggt gagcaagggc gaggaggata      960

actccgccat catcaaggag ttcctgcgct tcaaggtgca catggagggc tccgtgaacg     1020

gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg     1080

ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc     1140

agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga     1200

agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg     1260

tggtgaccgt gacccaggac tcctctctgc aggacggcga gttcatctac aaggtgaagc     1320

tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg     1380

aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga     1440

ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca     1500

agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc     1560

acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg     1620

gcggcatgga cgagctgtac aagtgatgaa agctttgctt tatttgtgaa atttgtgatg     1680

ctatgcttta tttgtaacca ttataagctg caataaacaa gtttaacaac aacaattgca     1740

ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagt aggtggggtc     1800

ggcggtcagg tgtcccagag ccaggggtct ggagggacct tccaccctca gtccctggca     1860

ggtcgggggg tgctgaggcg ggcctggccc tggcagccca ggggtcccgg agcgaggggt     1920

ctggagggac ctttcactct cagtccctgg caggtcgggg ggtgctgtgg caggcccagc     1980

cttggccccc agctctgccc cttaccctga gctgtgtggc tttgggcagc tcgaactcct     2040

gggttcctct ctgggcccca actcctcccc tggcccaagt cccctctttg ctcctgggca     2100

ggcaggacct ctgtcccctc tcagccggtc cttggggctg cgtgtttctg tagaatgacg     2160

ggtcaggctg gccagaaccc caaaccttgg ccgtggggag tctgcgtggc ggctctgcct     2220

tgcccaggca tccttggtcc tcactcgagt tttcctaagg atgggatgag ccccatgtgg     2280

gactaacctt ggctttacga cgtcaaagtt tagatgagct ggtgatattt ttctcattat     2340

atccaaagtg tacctgttcg agtgaggaca gttcttctgt ctccaggata cgtagataag     2400

tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc     2460

tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc     2520

tttgcccggg cggcctcagt gagcgagcga gcgcgccagc tggcgtaata gcgaagaggc     2580

ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gattccgttg     2640

caatggctgg cggtaatatt gttctggata ttaccagcaa ggccgatagt ttgagttctt     2700

ctactcaggc aagtgatgtt attactaatc aaagaagtat tgcgacaacg gttaatttgc     2760

gtgatggaca gactctttta ctcggtggcc tcactgatta taaaaacact tctcaggatt     2820

ctggcgtacc gttcctgtct aaaatccctt taatcggcct cctgtttagc tcccgctctg     2880

attctaacga ggaaagcacg ttatacgtgc tcgtcaaagc aaccatagta cgcgccctgt     2940

agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc     3000

agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc     3060

tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg     3120

cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga     3180

tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc     3240

caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg     3300

ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt     3360

aacaaaatat taacgtttac aatttaaata tttgcttata caatcttcct gtttttgggg     3420

cttttctgat tatcaaccgg ggtacatatg attgacatgc tagttttacg attaccgttc     3480

atcgattctc ttgtttgctc cagactctca ggcaatgacc tgatagcctt tgtagagacc     3540

tctcaaaaat agctaccctc tccggcatga atttatcagc tagaacggtt gaatatcata     3600

ttgatggtga tttgactgtc tccggccttt ctcacccgtt tgaatcttta cctacacatt     3660

actcaggcat tgcatttaaa atatatgagg gttctaaaaa tttttatcct tgcgttgaaa     3720

taaaggcttc tcccgcaaaa gtattacagg gtcataatgt ttttggtaca accgatttag     3780

ctttatgctc tgaggcttta ttgcttaatt ttgctaattc tttgccttgc ctgtatgatt     3840

tattggatgt tggaatcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc     3900

acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc     3960

ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc     4020

ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc     4080

accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat     4140

gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc     4200

tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg     4260

ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc     4320

ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt     4380

gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct     4440

caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac     4500

ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact     4560

cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa     4620

gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga     4680

taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt     4740

tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga     4800

agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg     4860

caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat     4920

ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat     4980

tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc     5040

agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga     5100

tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc     5160

agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag     5220

gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc     5280

gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt     5340

tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt     5400

gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat     5460

accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc     5520

accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa     5580

gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg     5640

ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag     5700

atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag     5760

gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa     5820

cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt     5880

gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg     5940

gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc     6000

tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac     6060

cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct     6120

ccccgcgcgt tggccgattc attaatg                                         6147


<210>  59
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab

<400>  59

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Arg 
            180                 185                 190         


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
        195                 200                 205             


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
    210                 215                 220                 


Arg Asp Phe Ala Ala Tyr Arg Ser 
225                 230         


<210>  60
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant PD-1.ile3.exon1_RD2_B1G2


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  60

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Gln Leu Glu Ile Arg Asn Val 
            20                  25                  30          


Asn Pro Asn Ile Pro Arg Tyr Lys Thr Arg Leu Arg Phe Glu Ile Asp 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Tyr Asn Gln Gly Asp Ser Tyr Val Lys Leu Arg 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly His Phe Gly Val Ile Leu Ala Lys Arg Arg Pro Ala Ser 
            180                 185                 190         


Pro Val Gln Val Arg Leu Arg Phe Ala Ile Gly Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Arg Ile 
    210                 215                 220                 


Arg Glu Lys Asn Ile Ser Glu Lys Ser Trp Leu Glu Phe Glu Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  61
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant PD-1.ile3.exon1_RD3_B1G2C4


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  61

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Gly Leu Ser Ile Leu Asn Arg 
            20                  25                  30          


Asn Arg Gly Thr Ala Arg Tyr His Thr Arg Leu Ser Phe Thr Ile Met 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Ser Ile Leu Asn Asn Gly Asp His Tyr Val Ser Leu Val 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  62
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant PD-1.ile3.exon1_RD3_B1 G2C11


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  62

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Gly Leu Ser Ile Leu Asn Arg 
            20                  25                  30          


Asn Arg Gly Thr Gly Arg Tyr His Thr Arg Leu Ser Phe Thr Ile Met 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Ser Ile Thr Asn Asn Gly Asp His Tyr Val Ser Leu Val 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  63
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant PD-1.ile3.exon1_RD3_B1 G2C5


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  63

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Gly Leu Ser Ile Leu Asn Arg 
            20                  25                  30          


Asn Arg Gly Thr Ala Arg Tyr His Thr Arg Leu Ser Phe Thr Ile Met 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Ser Ile Tyr Asn Asn Gly Asp His Tyr Val Ser Leu Glu 
65                  70                  75                  80  


Val Phe Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  64
<211>  943
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized megaTAL PD-1.ile3.exon1_RD1_B1G2 construct

<400>  64

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
465                 470                 475                 480 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                485                 490                 495     


Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
            500                 505                 510         


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
        515                 520                 525             


Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val 
    530                 535                 540                 


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
545                 550                 555                 560 


Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu 
                565                 570                 575     


Ser Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu 
            580                 585                 590         


Thr Asn Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala 
        595                 600                 605             


Met Asp Ala Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg 
    610                 615                 620                 


Arg Val Asn Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile 
625                 630                 635                 640 


Ser Arg Val Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile 
                645                 650                 655     


Leu Thr Gly Phe Ala Asp Ala Glu Gly Ser Phe Gly Leu Ser Ile Leu 
            660                 665                 670         


Asn Arg Asn Arg Gly Thr Ala Arg Tyr His Thr Arg Leu Ser Phe Thr 
        675                 680                 685             


Ile Met Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser 
    690                 695                 700                 


Thr Trp Lys Val Gly Ile Ile Thr Asn Asn Gly Asp His Tyr Val Thr 
705                 710                 715                 720 


Leu Arg Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe 
                725                 730                 735     


Glu Lys Tyr Pro Leu Val Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe 
            740                 745                 750         


Lys Gln Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn 
        755                 760                 765             


Gly Ile Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu 
    770                 775                 780                 


Asn Asp Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg 
785                 790                 795                 800 


Pro Leu Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe 
                805                 810                 815     


Thr Ser Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val 
            820                 825                 830         


Asn Ala Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile 
        835                 840                 845             


Arg Asp Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly 
    850                 855                 860                 


His Ile Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg 
865                 870                 875                 880 


Val Glu Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln 
                885                 890                 895     


Glu Asn Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys 
            900                 905                 910         


Lys Val Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly 
        915                 920                 925             


Leu Asp Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg 
    930                 935                 940             


<210>  65
<211>  2832
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthesized PD-1 Exon1 RD2_B1G2 megaTAL mRNA construct

<400>  65
augggaagcg cgccaccuaa gaagaaacgc aaagucgugg aucuacgcac gcucggcuac       60

agucagcagc agcaagagaa gaucaaaccg aaggugcguu cgacaguggc gcagcaccac      120

gaggcacugg ugggccaugg guuuacacac gcgcacaucg uugcgcucag ccaacacccg      180

gcagcguuag ggaccgucgc ugucacguau cagcacauaa ucacggcguu gccagaggcg      240

acacacgaag acaucguugg cgucggcaaa cagugguccg gcgcacgcgc ccuggaggcc      300

uugcucacgg augcggggga guugagaggu ccgccguuac aguuggacac aggccaacuu      360

gugaagauug caaaacgugg cggcgugacc gcaauggagg cagugcaugc aucgcgcaau      420

gcacugacgg gugccccccu gaaccuaacc ccugaucagg uagucgcuau agcuucaaac      480

aacgggggca agcaagcacu ggagaccguu caacgacucc ugccagugcu cugccaagac      540

cacggacuua cgccagauca ggugguugcu auugccucca acaauggcgg gaaacaagcg      600

uuggaaacug ugcagagacu guuaccuguc uugugucaag accacggccu cacgccagau      660

caggugguag ccauagcguc gaauggaggu gguaagcaag cccuugaaac gguccagcgu      720

cuucugccgg uguugugcca ggaccacgga cuaacgccgg aucaggucgu agccauugcu      780

ucaaauaacg gcggcaaaca ggcgcuagag acaguccagc gccucuugcc uguguuaugc      840

caggaucacg gcuuaacccc agaccaaguu guggcuauug caucuaacaa ugguggcaaa      900

caagccuugg agacagugca acgauuacug ccugucuuau gucaggauca uggccugacg      960

cccgaucagg uaguggcaau cgcaucuaau aauggaggua agcaagcacu ggagacuguc     1020

cagagauugu uacccguacu augucaagau caugguuuga cgccugauca gguuguugcg     1080

auagccagca acaacggagg gaaacaggcu cuugaaaccg uacagcgacu ucucccaguc     1140

uugugccaag aucacgggcu uacuccugau caagucguag cuaucgccag ccacgacggu     1200

gggaaacagg cccuggaaac cguacaacgu cuccucccag uacuuuguca agaccacggg     1260

uugacuccgg aucaagucgu cgcgaucgcg agcaauggag gggggaagca ggcgcuggaa     1320

acuguucaga gacugcugcc uguacuuugu caggaccaug gucugacacc ugaccaaguu     1380

guggcgauag ccaguaacaa ugggggaaaa caggcacuag agacgguuca aagguuguug     1440

cccguucugu gccaggacca cggcuugaca ccggaucagg ugguagcuau cgcuucacac     1500

gauggcggaa aacaggcuuu agaaacaguc caaagacuuc ucccaguccu uugucaggac     1560

cacggauuga cuccagauca agucguugcu auugcaagua augguggugg uaagcaagcu     1620

uuagaaaccg uacagaggcu uuugccagug cugugccagg accauggacu gaccccugau     1680

caagugguag caauugcauc ucaugaugga ggaaaacaag cucuggaaag cauuguggcc     1740

cagcugagcc ggccugaucc ggcguuggcc gcguugacca acgaccaccu cgucgccuug     1800

gccugccucg gcggacgucc ugccauggau gcagugaaaa agggauugcc gcacgcgccg     1860

gaauugauca gaagagucaa ucgccguauu ggcgaacgca cgucccaucg cguugcgaua     1920

ucuagagugg gaggaagcuc ucgcagagag uccaucaacc cauggauucu gacugguuuc     1980

gcugaugccg aaggaucauu cgggcuaagc auccucaaca gaaacagagg uacugcuaga     2040

uaccacacuc gacugucauu cacaaucaug cugcacaaca aggacaaauc gauucuggag     2100

aauauccagu cgacuuggaa ggucggcaua aucaccaaca acggcgacca cuacgucacc     2160

cugcgcguca cccguuucga agauuugaaa gugauuaucg accacuucga gaaauauccg     2220

cugguaacac agaaauuggg cgauuacaag uuguuuaaac aggcauucag cgucauggag     2280

aacaaagaac aucuuaagga gaaugggauu aaggagcucg uacgaaucaa agcuaagaug     2340

aauugggguc ucaaugacga auugaaaaaa gcauuuccag agaacauuag caaagagcgc     2400

ccccuuauca auaagaacau uccgaauuuc aaauggcugg cuggauucac aucuggugau     2460

ggcuccuucu ucgugcgccu aagaaagucu aauguuaaug cuagaguacg ugugcaacug     2520

guauucgaga ucucacagca caucagagac aagaaccuga ugaauucauu gauaacauac     2580

cuaggcugug gucacaucua cgagggaaac aaaucugagc gcaguuggcu ccaauucaga     2640

guagaaaaau ucagcgauau caacgacaag aucauuccgg uauuccagga aaauacucug     2700

auuggcguca aacucgagga cuuugaagau uggugcaagg uugccaaauu gaucgaagag     2760

aagaaacacc ugaccgaauc cgguuuggau gagauuaaga aaaucaagcu gaacaugaac     2820

aaaggucguu ga                                                         2832


<210>  66
<211>  2832
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthesized PD-1 Exon1 RD2_B1G2C4 megaTAL mRNA construct

<400>  66
augggaagcg cgccaccuaa gaagaaacgc aaagucgugg aucuacgcac gcucggcuac       60

agucagcagc agcaagagaa gaucaaaccg aaggugcguu cgacaguggc gcagcaccac      120

gaggcacugg ugggccaugg guuuacacac gcgcacaucg uugcgcucag ccaacacccg      180

gcagcguuag ggaccgucgc ugucacguau cagcacauaa ucacggcguu gccagaggcg      240

acacacgaag acaucguugg cgucggcaaa cagugguccg gcgcacgcgc ccuggaggcc      300

uugcucacgg augcggggga guugagaggu ccgccguuac aguuggacac aggccaacuu      360

gugaagauug caaaacgugg cggcgugacc gcaauggagg cagugcaugc aucgcgcaau      420

gcacugacgg gugccccccu gaaccuaacc ccugaucagg uagucgcuau agcuucaaac      480

aacgggggca agcaagcacu ggagaccguu caacgacucc ugccagugcu cugccaagac      540

cacggacuua cgccagauca ggugguugcu auugccucca acaauggcgg gaaacaagcg      600

uuggaaacug ugcagagacu guuaccuguc uugugucaag accacggccu cacgccagau      660

caggugguag ccauagcguc gaauggaggu gguaagcaag cccuugaaac gguccagcgu      720

cuucugccgg uguugugcca ggaccacgga cuaacgccgg aucaggucgu agccauugcu      780

ucaaauaacg gcggcaaaca ggcgcuagag acaguccagc gccucuugcc uguguuaugc      840

caggaucacg gcuuaacccc agaccaaguu guggcuauug caucuaacaa ugguggcaaa      900

caagccuugg agacagugca acgauuacug ccugucuuau gucaggauca uggccugacg      960

cccgaucagg uaguggcaau cgcaucuaau aauggaggua agcaagcacu ggagacuguc     1020

cagagauugu uacccguacu augucaagau caugguuuga cgccugauca gguuguugcg     1080

auagccagca acaacggagg gaaacaggcu cuugaaaccg uacagcgacu ucucccaguc     1140

uugugccaag aucacgggcu uacuccugau caagucguag cuaucgccag ccacgacggu     1200

gggaaacagg cccuggaaac cguacaacgu cuccucccag uacuuuguca agaccacggg     1260

uugacuccgg aucaagucgu cgcgaucgcg agcaauggag gggggaagca ggcgcuggaa     1320

acuguucaga gacugcugcc uguacuuugu caggaccaug gucugacacc ugaccaaguu     1380

guggcgauag ccaguaacaa ugggggaaaa caggcacuag agacgguuca aagguuguug     1440

cccguucugu gccaggacca cggcuugaca ccggaucagg ugguagcuau cgcuucacac     1500

gauggcggaa aacaggcuuu agaaacaguc caaagacuuc ucccaguccu uugucaggac     1560

cacggauuga cuccagauca agucguugcu auugcaagua augguggugg uaagcaagcu     1620

uuagaaaccg uacagaggcu uuugccagug cugugccagg accauggacu gaccccugau     1680

caagugguag caauugcauc ucaugaugga ggaaaacaag cucuggaaag cauuguggcc     1740

cagcugagcc ggccugaucc ggcguuggcc gcguugacca acgaccaccu cgucgccuug     1800

gccugccucg gcggacgucc ugccauggau gcagugaaaa agggauugcc gcacgcgccg     1860

gaauugauca gaagagucaa ucgccguauu ggcgaacgca cgucccaucg cguugcgaua     1920

ucuagagugg gaggaagcuc ucgcagagag uccaucaacc cauggauucu gacugguuuc     1980

gcugaugccg aaggaucauu cgggcuaagc auccucaaca gaaacagagg uacugcuaga     2040

uaccacacuc gacugucauu cacaaucaug cugcacaaca aggacaaauc gauucuggag     2100

aauauccagu cgacuuggaa ggucggcagc auccucaaca auggcgacca cuacgucucg     2160

cugguggucu accguuucga agauuugaaa gugauuaucg accacuucga gaaauauccg     2220

cugauaacac agaaauuggg cgauuacaag uuguuuaaac aggcauucag cgucauggag     2280

aacaaagaac aucuuaagga gaaugggauu aaggagcucg uacgaaucaa agcuaagaug     2340

aauugggguc ucaaugacga auugaaaaaa gcauuuccag agaacauuag caaagagcgc     2400

ccccuuauca auaagaacau uccgaauuuc aaauggcugg cuggauucac aucuggugau     2460

ggcuccuucu ucgugcgccu aagaaagucu aauguuaaug cuagaguacg ugugcaacug     2520

guauucgaga ucucacagca caucagagac aagaaccuga ugaauucauu gauaacauac     2580

cuaggcugug gucacaucua cgagggaaac aaaucugagc gcaguuggcu ccaauucaga     2640

guagaaaaau ucagcgauau caacgacaag aucauuccgg uauuccagga aaauacucug     2700

auuggcguca aacucgagga cuuugaagau uggugcaagg uugccaaauu gaucgaagag     2760

aagaaacacc ugaccgaauc cgguuuggau gagauuaaga aaaucaagcu gaacaugaac     2820

aaaggucguu ga                                                         2832


<210>  67
<211>  2832
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthesized PD-1 Exon1 RD2_B1G2C11 megaTAL mRNA construct

<400>  67
augggaagcg cgccaccuaa gaagaaacgc aaagucgugg aucuacgcac gcucggcuac       60

agucagcagc agcaagagaa gaucaaaccg aaggugcguu cgacaguggc gcagcaccac      120

gaggcacugg ugggccaugg guuuacacac gcgcacaucg uugcgcucag ccaacacccg      180

gcagcguuag ggaccgucgc ugucacguau cagcacauaa ucacggcguu gccagaggcg      240

acacacgaag acaucguugg cgucggcaaa cagugguccg gcgcacgcgc ccuggaggcc      300

uugcucacgg augcggggga guugagaggu ccgccguuac aguuggacac aggccaacuu      360

gugaagauug caaaacgugg cggcgugacc gcaauggagg cagugcaugc aucgcgcaau      420

gcacugacgg gugccccccu gaaccuaacc ccugaucagg uagucgcuau agcuucaaac      480

aacgggggca agcaagcacu ggagaccguu caacgacucc ugccagugcu cugccaagac      540

cacggacuua cgccagauca ggugguugcu auugccucca acaauggcgg gaaacaagcg      600

uuggaaacug ugcagagacu guuaccuguc uugugucaag accacggccu cacgccagau      660

caggugguag ccauagcguc gaauggaggu gguaagcaag cccuugaaac gguccagcgu      720

cuucugccgg uguugugcca ggaccacgga cuaacgccgg aucaggucgu agccauugcu      780

ucaaauaacg gcggcaaaca ggcgcuagag acaguccagc gccucuugcc uguguuaugc      840

caggaucacg gcuuaacccc agaccaaguu guggcuauug caucuaacaa ugguggcaaa      900

caagccuugg agacagugca acgauuacug ccugucuuau gucaggauca uggccugacg      960

cccgaucagg uaguggcaau cgcaucuaau aauggaggua agcaagcacu ggagacuguc     1020

cagagauugu uacccguacu augucaagau caugguuuga cgccugauca gguuguugcg     1080

auagccagca acaacggagg gaaacaggcu cuugaaaccg uacagcgacu ucucccaguc     1140

uugugccaag aucacgggcu uacuccugau caagucguag cuaucgccag ccacgacggu     1200

gggaaacagg cccuggaaac cguacaacgu cuccucccag uacuuuguca agaccacggg     1260

uugacuccgg aucaagucgu cgcgaucgcg agcaauggag gggggaagca ggcgcuggaa     1320

acuguucaga gacugcugcc uguacuuugu caggaccaug gucugacacc ugaccaaguu     1380

guggcgauag ccaguaacaa ugggggaaaa caggcacuag agacgguuca aagguuguug     1440

cccguucugu gccaggacca cggcuugaca ccggaucagg ugguagcuau cgcuucacac     1500

gauggcggaa aacaggcuuu agaaacaguc caaagacuuc ucccaguccu uugucaggac     1560

cacggauuga cuccagauca agucguugcu auugcaagua augguggugg uaagcaagcu     1620

uuagaaaccg uacagaggcu uuugccagug cugugccagg accauggacu gaccccugau     1680

caagugguag caauugcauc ucaugaugga ggaaaacaag cucuggaaag cauuguggcc     1740

cagcugagcc ggccugaucc ggcguuggcc gcguugacca acgaccaccu cgucgccuug     1800

gccugccucg gcggacgucc ugccauggau gcagugaaaa agggauugcc gcacgcgccg     1860

gaauugauca gaagagucaa ucgccguauu ggcgaacgca cgucccaucg cguugcgaua     1920

ucuagagugg gaggaagcuc ucgcagagag uccaucaacc cauggauucu gacugguuuc     1980

gcugaugccg aaggaucauu cgggcuaagc auccucaaca gaaacagagg uacugguaga     2040

uaccacacuc gacugucauu cacaaucaug cugcacaaca aggacaaauc gauucuggag     2100

aauauccagu cgacuuggaa ggucggcucg aucacgaaca acggcgacca cuacgucagc     2160

cuggucgucu accguuucga agauuugaaa gugauuaucg accacuucga gaaauauccg     2220

cugauaacac agaaauuggg cgauuacaag uuguuuaaac aggcauucag cgucauggag     2280

aacaaagaac aucuuaagga gaaugggauu aaggagcucg uacgaaucaa agcuaagaug     2340

aauugggguc ucaaugacga auugaaaaaa gcauuuccag agaacauuag caaagagcgc     2400

ccccuuauca auaagaacau uccgaauuuc aaauggcugg cuggauucac aucuggugau     2460

ggcuccuucu ucgugcgccu aagaaagucu aauguuaaug cuagaguacg ugugcaacug     2520

guauucgaga ucucacagca caucagagac aagaaccuga ugaauucauu gauaacauac     2580

cuaggcugug gucacaucua cgagggaaac aaaucugagc gcaguuggcu ccaauucaga     2640

guagaaaaau ucagcgauau caacgacaag aucauuccgg uauuccagga aaauacucug     2700

auuggcguca aacucgagga cuuugaagau uggugcaagg uugccaaauu gaucgaagag     2760

aagaaacacc ugaccgaauc cgguuuggau gagauuaaga aaaucaagcu gaacaugaac     2820

aaaggucguu ga                                                         2832


<210>  68
<211>  2832
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthesized PD-1 Exon1 RD2_B1G2C5 megaTAL mRNA construct

<400>  68
augggaagcg cgccaccuaa gaagaaacgc aaagucgugg aucuacgcac gcucggcuac       60

agucagcagc agcaagagaa gaucaaaccg aaggugcguu cgacaguggc gcagcaccac      120

gaggcacugg ugggccaugg guuuacacac gcgcacaucg uugcgcucag ccaacacccg      180

gcagcguuag ggaccgucgc ugucacguau cagcacauaa ucacggcguu gccagaggcg      240

acacacgaag acaucguugg cgucggcaaa cagugguccg gcgcacgcgc ccuggaggcc      300

uugcucacgg augcggggga guugagaggu ccgccguuac aguuggacac aggccaacuu      360

gugaagauug caaaacgugg cggcgugacc gcaauggagg cagugcaugc aucgcgcaau      420

gcacugacgg gugccccccu gaaccuaacc ccugaucagg uagucgcuau agcuucaaac      480

aacgggggca agcaagcacu ggagaccguu caacgacucc ugccagugcu cugccaagac      540

cacggacuua cgccagauca ggugguugcu auugccucca acaauggcgg gaaacaagcg      600

uuggaaacug ugcagagacu guuaccuguc uugugucaag accacggccu cacgccagau      660

caggugguag ccauagcguc gaauggaggu gguaagcaag cccuugaaac gguccagcgu      720

cuucugccgg uguugugcca ggaccacgga cuaacgccgg aucaggucgu agccauugcu      780

ucaaauaacg gcggcaaaca ggcgcuagag acaguccagc gccucuugcc uguguuaugc      840

caggaucacg gcuuaacccc agaccaaguu guggcuauug caucuaacaa ugguggcaaa      900

caagccuugg agacagugca acgauuacug ccugucuuau gucaggauca uggccugacg      960

cccgaucagg uaguggcaau cgcaucuaau aauggaggua agcaagcacu ggagacuguc     1020

cagagauugu uacccguacu augucaagau caugguuuga cgccugauca gguuguugcg     1080

auagccagca acaacggagg gaaacaggcu cuugaaaccg uacagcgacu ucucccaguc     1140

uugugccaag aucacgggcu uacuccugau caagucguag cuaucgccag ccacgacggu     1200

gggaaacagg cccuggaaac cguacaacgu cuccucccag uacuuuguca agaccacggg     1260

uugacuccgg aucaagucgu cgcgaucgcg agcaauggag gggggaagca ggcgcuggaa     1320

acuguucaga gacugcugcc uguacuuugu caggaccaug gucugacacc ugaccaaguu     1380

guggcgauag ccaguaacaa ugggggaaaa caggcacuag agacgguuca aagguuguug     1440

cccguucugu gccaggacca cggcuugaca ccggaucagg ugguagcuau cgcuucacac     1500

gauggcggaa aacaggcuuu agaaacaguc caaagacuuc ucccaguccu uugucaggac     1560

cacggauuga cuccagauca agucguugcu auugcaagua augguggugg uaagcaagcu     1620

uuagaaaccg uacagaggcu uuugccagug cugugccagg accauggacu gaccccugau     1680

caagugguag caauugcauc ucaugaugga ggaaaacaag cucuggaaag cauuguggcc     1740

cagcugagcc ggccugaucc ggcguuggcc gcguugacca acgaccaccu cgucgccuug     1800

gccugccucg gcggacgucc ugccauggau gcagugaaaa agggauugcc gcacgcgccg     1860

gaauugauca gaagagucaa ucgccguauu ggcgaacgca cgucccaucg cguugcgaua     1920

ucuagagugg gaggaagcuc ucgcagagag uccaucaacc cauggauucu gacugguuuc     1980

gcugaugccg aaggaucauu cgggcuaagc auccucaaca gaaacagagg uacugcuaga     2040

uaccacacuc gacugucauu cacaaucaug cugcacaaca aggacaaauc gauucuggag     2100

aauauccagu cgacuuggaa ggucggcucg aucuacaaca acggcgacca cuacgucucg     2160

cuggaggucu uccguuucga agauuugaaa gugauuaucg accacuucga gaaauauccg     2220

cugauaacac agaaauuggg cgauuacaag uuguuuaaac aggcauucag cgucauggag     2280

aacaaagaac aucuuaagga gaaugggauu aaggagcucg uacgaaucaa agcuaagaug     2340

aauugggguc ucaaugacga auugaaaaaa gcauuuccag agaacauuag caaagagcgc     2400

ccccuuauca auaagaacau uccgaauuuc aaauggcugg cuggauucac aucuggugau     2460

ggcuccuucu ucgugcgccu aagaaagucu aauguuaaug cuagaguacg ugugcaacug     2520

guauucgaga ucucacagca caucagagac aagaaccuga ugaauucauu gauaacauac     2580

cuaggcugug gucacaucua cgagggaaac aaaucugagc gcaguuggcu ccaauucaga     2640

guagaaaaau ucagcgauau caacgacaag aucauuccgg uauuccagga aaauacucug     2700

auuggcguca aacucgagga cuuugaagau uggugcaagg uugccaaauu gaucgaagag     2760

aagaaacacc ugaccgaauc cgguuuggau gagauuaaga aaaucaagcu gaacaugaac     2820

aaaggucguu ga                                                         2832


<210>  69
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  69

Gly Gly Gly 
1           


<210>  70
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  70

Asp Gly Gly Gly Ser 
1               5   


<210>  71
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  71

Thr Gly Glu Lys Pro 
1               5   


<210>  72
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  72

Gly Gly Arg Arg 
1               


<210>  73
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  73

Gly Gly Gly Gly Ser 
1               5   


<210>  74
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  74

Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Val Asp 
1               5                   10                  


<210>  75
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  75

Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 
1               5                   10                  15      


Leu Asp 
        


<210>  76
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  76

Gly Gly Arg Arg Gly Gly Gly Ser 
1               5               


<210>  77
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  77

Leu Arg Gln Arg Asp Gly Glu Arg Pro 
1               5                   


<210>  78
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  78

Leu Arg Gln Lys Asp Gly Gly Gly Ser Glu Arg Pro 
1               5                   10          


<210>  79
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  79

Leu Arg Gln Lys Asp Gly Gly Gly Ser Gly Gly Gly Ser Glu Arg Pro 
1               5                   10                  15      


<210>  80
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa is any amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa = Gly or Ser

<400>  80

Glu Xaa Xaa Tyr Xaa Gln Xaa 
1               5           


<210>  81
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  81

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  82
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  82

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  83
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  83

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  84
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  84

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  85
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  85

Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10                  


<210>  86
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  86

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  87
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  87

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  88
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  88

Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10              


<210>  89
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  89

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro 
            20              


<210>  90
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  90

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  91
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  91

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  92
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  92

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210>  93
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  93

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  94
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  94

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  95
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  95

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  96
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  96

Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  97
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  97

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  98
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  98

Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
1               5                   10                  15      


Pro 
    


<210>  99
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  99

Gln Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  100
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  100

Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
1               5                   10                  15      


Asp Val Glu Ser Asn Pro Gly Pro 
            20                  


<210>  101
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  101

Val Thr Glu Leu Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro 
1               5                   10                  15      


Arg Pro Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys 
            20                  25                  30          


Ile Val Ala Pro Val Lys Gln Thr 
        35                  40  


<210>  102
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  102

Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  103
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  103

Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys Ile Val 
1               5                   10                  15      


Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
            20                  25                  30          


Asp Val Glu Ser Asn Pro Gly Pro 
        35                  40  


<210>  104
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  104

Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Thr Leu 
1               5                   10                  15      


Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
            20                  25                  30          


Pro 
    


<210>  105
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus Kozak sequence

<400>  105
gccrccatgg                                                              10


<210>  106
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  106

Glu Gln Thr Glu Tyr Ala Thr Ile 
1               5               


<210>  107
<211>  43
<212>  DNA
<213>  Homo sapiens

<400>  107
tgagcagacg gagtatgcca ccattgtctt tcctagcgga atg                         43


<210>  108
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab

<400>  108
cattccgcta ggaaagacaa tggtggcata ctccgtctgc tca                         43


<210>  109
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab


<220>
<221>  misc_feature
<222>  (14)..(22)
<223>  n is A, C, G or T

<400>  109
aatggtggca tacnnnnnnn nn                                                22


<210>  110
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  n is a, c, g, or t

<400>  110
nnnnnnnnna tactccgtct gc                                                22


<210>  111
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab

<400>  111
aatggtggca tactccgtct gc                                                22


<210>  112
<211>  43
<212>  DNA
<213>  Homo sapiens

<400>  112
attccgctag gaaagacaat ggtggcatac tccgtctgct cag                         43


<210>  113
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab

<400>  113
atttcgttag gaaagataat ggtggtatat ttcgtttgtt tag                         43


<210>  114
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  114

Met Gln Ile Pro Gln Ala Pro 
1               5           


<210>  115
<211>  44
<212>  DNA
<213>  Homo sapiens

<400>  115
ctctggtggg gctgctccag gcatgcagat cccacaggcg ccct                        44


<210>  116
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab

<400>  116
agggcgcctg tgggatctgc atccctggag cagccccacc agag                        44


<210>  117
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  117

Asn Gly Arg Asp Phe His Met Ser 
1               5               


<210>  118
<211>  43
<212>  DNA
<213>  Homo sapiens

<400>  118
gtgtcacaca actgcccaac gggcgtgact tccacatgag cgt                         43


<210>  119
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab

<400>  119
acgctcatgt ggaagtcacg cccgttgggc agttgtgtga cac                         43


<210>  120
<211>  32
<212>  DNA
<213>  Homo sapiens

<400>  120
gctccaggca tgcagatccc acaggcgccc tg                                     32


<210>  121
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  made in lab

<400>  121
actccaaaca tacaaatccc acaaacaccc ta                                     32


<210>  122
<211>  31
<212>  DNA
<213>  Homo sapiens

<400>  122
gcccaacggg cgtgacttcc acatgagcgt g                                      31


<210>  123
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab

<400>  123
acccaacgaa cgtaacttcc acataaacgt a                                      31


