                         SEQUENCE LISTING

<110>  Massachusetts Institute of Technology
 
<120>  TRANSCRIPTIONAL SENSOR FOR BILE ACIDS IN BACTEROIDES THETAIOTAOMICRON

<130>  M0656.70392WO00

<150>  US 62/393,371
<151>  2016-09-12

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  630
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BreR-Bt codon optimized for use in B. thetaiotamicron

<400>  1
atgaaattgt ctgaacagaa acgtttggct ttgatcgaag ctgctaaaga agaattcact       60

cagttcggtt tccacgctgc taacatggac cgtgtatgtg aacgtgctgg tacttctaaa      120

cgtactttgt accgtcactt cacttctaaa gaattgttgt tcatcgaagt aatcaacttg      180

ttggtagctc agccgcacaa agtaggtttc gaataccagt ctactcgttc tttggctgac      240

cagttgcacg actacttcgc tgctaaaatc gacttgttgt accgtactat cggtttggac      300

gtattgcgta tgatcgtagg tgaattcgta cgtgacccgg ctttgactca gcagtacttg      360

gctttgatgg gtactcagga cactgctttg actgcttggt tgcaggctgc tatcaaagac      420

ggtaaattga tcgaaaaaga agtagctccg atggctacta ctttgatgaa cttgttccac      480

ggtcagttct tgtggccgca gttgttggct gctgtagaat tgccggacgc taaacagcag      540

cagatcatga tggacgaaat catccgtgta ttcgtattgt cttacggtgt atcttctccg      600

tctcacttgt ctatcgaatt gaaaccgtaa                                       630


<210>  2
<211>  612
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VFA0359-Bt codon optimized for use in B. thetaiotamicron

<400>  2
atgcagaaaa aattgactcg ttctcagcag aaacacttgg acatcatcaa cgctgctaaa       60

gaagaattca tcgaattcgg tttcttggct gctaacatgg accgtatcac ttcttctgct      120

gaagtatcta aacgtacttt gtaccgtcac ttcgaatcta aagaagtatt gttcgaatct      180

gtattgacta tcatcaacga ctctgtaaac gaatctatct cttaccactt cgacccgaac      240

aaatctactg aagaacagtt gactgaaatc gcttacaaag aaatcgacgt attgtacaaa      300

acttacggta tcgctttggc tcgtactatc gtaatggaat tcttgcgtca gccggaaatg      360

gctaaaactt tgatccagaa catctactct atccgtgcta tcactcagtg gttccgttct      420

gctatcgaag ctaaacgttt gaaagacgct gacccgaaat tgatgactga cgtatacgta      480

tctttgttcc agggtttgtt cttctggccg caggtaatgc acttggactt ggaaccgcac      540

ggtgaagaat tgtctcagaa aatcgaaact ttgactacta tcttcttgca gtcttacggt      600

gtagctgaat aa                                                          612


<210>  3
<211>  283
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BT1311 Constitutive promoter+RBS

<400>  3
tgatctggaa gaagcaatga aagctgctgt taagtctccg aatcaggtat tgttcctgac       60

aggtgtattc ccatccggta aacgcggata ctttgcagtt gatctgactc aggaataaat      120

tataaattaa ggtaagaaga ttgtaggata agctaatgaa atagaaaaag gatgccgtca      180

cacaacttgt cggcattctt ttttgtttta ttagttgaaa atatagtgaa aaagttgcct      240

aaatatgtat gttaacaaat tatttgtcgt aactttgcac tcc                        283


<210>  4
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bacteroides consensus promoter sequence


<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  n is a, c, g, or t

<400>  4
tttgtanntt tg                                                           12


<210>  5
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BreR consensus sequence


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (10)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n is a, c, g, or t

<400>  5
aangtanacn nnnnngtnta cntt                                              24


<210>  6
<211>  116
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PbreR (Bile acid inducible promoter)

<400>  6
ttacaaagaa aattcgacaa actgttattt ttctatctat ttaaatgtac acccgtttgt       60

ttactttact ttagttatgt acctttgtcg gcaatgtaca cccgaaagtt tacttt          116


<210>  7
<211>  122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PcfxA (Constitutive promoter+RBS)

<400>  7
ttacaaagaa aattcgacaa actgttattt ttctatctat ttatttgggt gggaaacttt       60

agttatgtac ctttgtcggc aaataaagat attctcgtca aacaaatata aataatataa      120

ac                                                                     122


<210>  8
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rpiL* (RBS)

<400>  8
cgcattttaa aataaaataa attatttatt taattaaacg aat                         43


<210>  9
<211>  516
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NanoLuc Luciferase reporter

<400>  9
atggttttta ctctggaaga ttttgttggc gattggcgtc agaccgcggg ttataatttg       60

gatcaagtcc tggaacaggg tggcgtaagc tctctgttcc agaacctggg tgtgagcgtg      120

acgccgattc agcgcatcgt tctgtccggc gagaacggtc tgaaaattga tattcatgtg      180

atcatcccgt acgaaggcct gagcggtgac caaatgggtc aaatcgagaa aatctttaaa      240

gtcgtctacc cagttgacga tcaccacttc aaggttatct tgcattacgg tacgctggtg      300

attgatggtg tgaccccgaa tatgattgac tatttcggcc gtccgtatga aggcattgcc      360

gtttttgacg gtaaaaagat caccgtcacc ggtaccctgt ggaatggcaa taagattatt      420

gacgagcgtc tgattaaccc ggacggcagc ctgctgttcc gcgtgaccat caacggtgtc      480

acgggttggc gtctgtgcga gcgcatcctg gcataa                                516


<210>  10
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  operator in the -33 element

<400>  10
aatgtacacc cgtttgttta cttt                                              24


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  operator downstream of the -7 element

<400>  11
aatgtacacc cgaaagttta cttt                                              24


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oMT113

<400>  12
cgctactctc tacgttcaac c                                                 21


<210>  13
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oMT114

<400>  13
tgaaatacag tgtaattgtg gcg                                               23


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oMT115

<400>  14
ttcacttcca tcccggttcg                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oMT116

<400>  15
cacatcgcca gggtccatta                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oMT242

<400>  16
ccaacaacaa gcacactcca                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oMT243

<400>  17
tgcgaatggt agaaaagccc                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oMT244

<400>  18
gacctgcaaa cgaccaaagt                                                   20


<210>  19
<211>  579
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BM3R1 coding sequence

<400>  19
atggaaagca ccccgaccaa acagaaagca atttttagcg caagcctgct gctgtttgca       60

gaacgtggtt ttgatgcaac caccatgccg atgattgcag aaaatgcaaa agttggtgca      120

ggcaccattt atcgctattt caaaaacaaa gaaagcctgg tgaacgaact gtttcagcag      180

catgttaatg aatttctgca gtgtattgaa agcggtctgg caaatgaacg tgatggttat      240

cgtgatggct ttcatcacat ttttgaaggt atggtgacct ttaccaaaaa tcatccgcgt      300

gcactgggtt ttatcaaaac ccatagccag ggcacctttc tgaccgaaga aagccgtctg      360

gcatatcaga aactggttga atttgtgtgc accttttttc gtgaaggtca gaaacagggt      420

gtgattcgta atctgccgga aaatgcactg attgcaattc tgtttggcag ctttatggaa      480

gtgtatgaaa tgatcgagaa cgattatctg agcctgaccg atgaactgct gaccggtgtt      540

gaagaaagcc tgtgggcagc actgagccgt cagagctaa                             579


