                         SEQUENCE LISTING

<110>  National University of Singapore
 
<120>  Compositions and Methods for Protein Expression and Delivery

<130>  FP9082

<150>  US 62/405,613
<151>  2016-10-07

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  173
<212>  PRT
<213>  Archaeoglobus fulgidus

<400>  1

Met Ala Ser Ile Ser Glu Lys Met Val Glu Ala Leu Asn Arg Gln Ile 
1               5                   10                  15      


Asn Ala Glu Ile Tyr Ser Ala Tyr Leu Tyr Leu Ser Met Ala Ser Tyr 
            20                  25                  30          


Phe Asp Ser Ile Gly Leu Lys Gly Phe Ser Asn Trp Met Arg Val Gln 
        35                  40                  45              


Trp Gln Glu Glu Leu Met His Ala Met Lys Met Phe Asp Phe Val Ser 
    50                  55                  60                  


Glu Arg Gly Gly Arg Val Lys Leu Tyr Ala Val Glu Glu Pro Pro Ser 
65                  70                  75                  80  


Glu Trp Asp Ser Pro Leu Ala Ala Phe Glu His Val Tyr Glu His Glu 
                85                  90                  95      


Val Asn Val Thr Lys Arg Ile His Glu Leu Val Glu Met Ala Met Gln 
            100                 105                 110         


Glu Lys Asp Phe Ala Thr Tyr Asn Phe Leu Gln Trp Tyr Val Ala Glu 
        115                 120                 125             


Gln Val Glu Glu Glu Ala Ser Ala Leu Asp Ile Val Glu Lys Leu Arg 
    130                 135                 140                 


Leu Ile Gly Glu Asp Lys Arg Ala Leu Leu Phe Leu Asp Lys Glu Leu 
145                 150                 155                 160 


Ser Leu Arg Gln Phe Thr Pro Pro Ala Glu Glu Glu Lys 
                165                 170             


<210>  2
<211>  166
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AfFtn subunit with truncation of unstructured C-terminus

<400>  2

Met Ala Ser Ile Ser Glu Lys Met Val Glu Ala Leu Asn Arg Gln Ile 
1               5                   10                  15      


Asn Ala Glu Ile Tyr Ser Ala Tyr Leu Tyr Leu Ser Met Ala Ser Tyr 
            20                  25                  30          


Phe Asp Ser Ile Gly Leu Lys Gly Phe Ser Asn Trp Met Arg Val Gln 
        35                  40                  45              


Trp Gln Glu Glu Leu Met His Ala Met Lys Met Phe Asp Phe Val Ser 
    50                  55                  60                  


Glu Arg Gly Gly Arg Val Lys Leu Tyr Ala Val Glu Glu Pro Pro Ser 
65                  70                  75                  80  


Glu Trp Asp Ser Pro Leu Ala Ala Phe Glu His Val Tyr Glu His Glu 
                85                  90                  95      


Val Asn Val Thr Lys Arg Ile His Glu Leu Val Glu Met Ala Met Gln 
            100                 105                 110         


Glu Lys Asp Phe Ala Thr Tyr Asn Phe Leu Gln Trp Tyr Val Ala Glu 
        115                 120                 125             


Gln Val Glu Glu Glu Ala Ser Ala Leu Asp Ile Val Glu Lys Leu Arg 
    130                 135                 140                 


Leu Ile Gly Glu Asp Lys Arg Ala Leu Leu Phe Leu Asp Lys Glu Leu 
145                 150                 155                 160 


Ser Leu Arg Gln Thr Ser 
                165     


<210>  3
<211>  166
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AfFtn net positive variant subunit with truncation of 
       unstructured  C-terminus

<400>  3

Met Ala Ser Ile Ser Glu Lys Met Val Glu Ala Leu Asn Arg Gln Ile 
1               5                   10                  15      


Asn Ala Glu Ile Tyr Ser Ala Tyr Leu Tyr Leu Ser Met Ala Ser Tyr 
            20                  25                  30          


Phe Asp Ser Ile Gly Leu Lys Gly Phe Ser Asn Trp Met Arg Val Gln 
        35                  40                  45              


Trp Gln Glu Glu Leu Met His Ala Met Lys Met Phe Asp Phe Val Ser 
    50                  55                  60                  


Lys Arg Gly Gly Arg Val Lys Leu Tyr Ala Val Glu Glu Pro Pro Ser 
65                  70                  75                  80  


Glu Trp Asp Ser Pro Leu Ala Ala Phe Glu His Val Tyr Glu His Glu 
                85                  90                  95      


Val Asn Val Thr Lys Arg Ile His Glu Leu Val Glu Met Ala Met Gln 
            100                 105                 110         


Glu Lys Asp Phe Ala Thr Tyr Asn Phe Leu Gln Trp Tyr Val Ala Lys 
        115                 120                 125             


Gln Val Lys Glu Glu Ala Ser Ala Leu Ala Ile Val Glu Lys Leu Arg 
    130                 135                 140                 


Leu Ile Gly Glu Asp Lys Arg Ala Leu Leu Phe Leu Asp Lys Glu Leu 
145                 150                 155                 160 


Ser Leu Arg Gln Thr Ser 
                165     


<210>  4
<211>  166
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AfFtn net neutral variant subunit with truncation of unstructured
       C-terminus

<400>  4

Met Ala Ser Ile Ser Glu Lys Met Val Glu Ala Leu Asn Arg Gln Ile 
1               5                   10                  15      


Asn Ala Glu Ile Tyr Ser Ala Tyr Leu Tyr Leu Ser Met Ala Ser Tyr 
            20                  25                  30          


Phe Asp Ser Ile Gly Leu Lys Gly Phe Ser Asn Trp Met Arg Val Gln 
        35                  40                  45              


Trp Gln Glu Glu Leu Met His Ala Met Lys Met Phe Asp Phe Val Ser 
    50                  55                  60                  


Gln Arg Gly Gly Arg Val Lys Leu Tyr Ala Val Glu Glu Pro Pro Ser 
65                  70                  75                  80  


Glu Trp Asp Ser Pro Leu Ala Ala Phe Glu His Val Tyr Glu His Glu 
                85                  90                  95      


Val Asn Val Thr Lys Arg Ile His Glu Leu Val Glu Met Ala Met Gln 
            100                 105                 110         


Glu Lys Asp Phe Ala Thr Tyr Asn Phe Leu Gln Trp Tyr Val Ala Glu 
        115                 120                 125             


Gln Val Glu Glu Glu Ala Ser Ala Leu Ala Ile Val Glu Lys Leu Arg 
    130                 135                 140                 


Leu Ile Gly Glu Asp Lys Arg Ala Leu Leu Phe Leu Asp Lys Glu Leu 
145                 150                 155                 160 


Ser Leu Arg Gln Thr Ser 
                165     


<210>  5
<211>  166
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AfFtn subunit with truncation of unstructured  C-terminus

       and F116H mutation

<400>  5

Met Ala Ser Ile Ser Glu Lys Met Val Glu Ala Leu Asn Arg Gln Ile 
1               5                   10                  15      


Asn Ala Glu Ile Tyr Ser Ala Tyr Leu Tyr Leu Ser Met Ala Ser Tyr 
            20                  25                  30          


Phe Asp Ser Ile Gly Leu Lys Gly Phe Ser Asn Trp Met Arg Val Gln 
        35                  40                  45              


Trp Gln Glu Glu Leu Met His Ala Met Lys Met Phe Asp Phe Val Ser 
    50                  55                  60                  


Glu Arg Gly Gly Arg Val Lys Leu Tyr Ala Val Glu Glu Pro Pro Ser 
65                  70                  75                  80  


Glu Trp Asp Ser Pro Leu Ala Ala Phe Glu His Val Tyr Glu His Glu 
                85                  90                  95      


Val Asn Val Thr Lys Arg Ile His Glu Leu Val Glu Met Ala Met Gln 
            100                 105                 110         


Glu Lys Asp His Ala Thr Tyr Asn Phe Leu Gln Trp Tyr Val Ala Glu 
        115                 120                 125             


Gln Val Glu Glu Glu Ala Ser Ala Leu Asp Ile Val Glu Lys Leu Arg 
    130                 135                 140                 


Leu Ile Gly Glu Asp Lys Arg Ala Leu Leu Phe Leu Asp Lys Glu Leu 
145                 150                 155                 160 


Ser Leu Arg Gln Thr Ser 
                165     


<210>  6
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial peptide #1

<400>  6

Tyr His Trp Tyr Gly Tyr Thr Pro Gln Asn Val Ile 
1               5                   10          


<210>  7
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial peptide #2

<400>  7

Leu Ala Arg Leu Leu Thr 
1               5       


<210>  8
<211>  166
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AfFtn net positive variant subunit with truncation of 
       unstructured  C-terminus

and F116H mutation

<400>  8

Met Ala Ser Ile Ser Glu Lys Met Val Glu Ala Leu Asn Arg Gln Ile 
1               5                   10                  15      


Asn Ala Glu Ile Tyr Ser Ala Tyr Leu Tyr Leu Ser Met Ala Ser Tyr 
            20                  25                  30          


Phe Asp Ser Ile Gly Leu Lys Gly Phe Ser Asn Trp Met Arg Val Gln 
        35                  40                  45              


Trp Gln Glu Glu Leu Met His Ala Met Lys Met Phe Asp Phe Val Ser 
    50                  55                  60                  


Lys Arg Gly Gly Arg Val Lys Leu Tyr Ala Val Glu Glu Pro Pro Ser 
65                  70                  75                  80  


Glu Trp Asp Ser Pro Leu Ala Ala Phe Glu His Val Tyr Glu His Glu 
                85                  90                  95      


Val Asn Val Thr Lys Arg Ile His Glu Leu Val Glu Met Ala Met Gln 
            100                 105                 110         


Glu Lys Asp His Ala Thr Tyr Asn Phe Leu Gln Trp Tyr Val Ala Lys 
        115                 120                 125             


Gln Val Lys Glu Glu Ala Ser Ala Leu Ala Ile Val Glu Lys Leu Arg 
    130                 135                 140                 


Leu Ile Gly Glu Asp Lys Arg Ala Leu Leu Phe Leu Asp Lys Glu Leu 
145                 150                 155                 160 


Ser Leu Arg Gln Thr Ser 
                165     


