                         SEQUENCE LISTING

<110>  The United States of America as represented by the 
       Secretary, Department of Health and human Services
 
<120>  PREFUSION CORONAVIRUS SPIKE PROTEINS AND THEIR USE

<130>  4239-97383-02

<150>  US 62/412,703
<151>  2016-10-25

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  1353
<212>  PRT
<213>  middle east respiratory syndrome coronavirus

<400>  1

Met Ile His Ser Val Phe Leu Leu Met Phe Leu Leu Thr Pro Thr Glu 
1               5                   10                  15      


Ser Tyr Val Asp Val Gly Pro Asp Ser Val Lys Ser Ala Cys Ile Glu 
            20                  25                  30          


Val Asp Ile Gln Gln Thr Phe Phe Asp Lys Thr Trp Pro Arg Pro Ile 
        35                  40                  45              


Asp Val Ser Lys Ala Asp Gly Ile Ile Tyr Pro Gln Gly Arg Thr Tyr 
    50                  55                  60                  


Ser Asn Ile Thr Ile Thr Tyr Gln Gly Leu Phe Pro Tyr Gln Gly Asp 
65                  70                  75                  80  


His Gly Asp Met Tyr Val Tyr Ser Ala Gly His Ala Thr Gly Thr Thr 
                85                  90                  95      


Pro Gln Lys Leu Phe Val Ala Asn Tyr Ser Gln Asp Val Lys Gln Phe 
            100                 105                 110         


Ala Asn Gly Phe Val Val Arg Ile Gly Ala Ala Ala Asn Ser Thr Gly 
        115                 120                 125             


Thr Val Ile Ile Ser Pro Ser Thr Ser Ala Thr Ile Arg Lys Ile Tyr 
    130                 135                 140                 


Pro Ala Phe Met Leu Gly Ser Ser Val Gly Asn Phe Ser Asp Gly Lys 
145                 150                 155                 160 


Met Gly Arg Phe Phe Asn His Thr Leu Val Leu Leu Pro Asp Gly Cys 
                165                 170                 175     


Gly Thr Leu Leu Arg Ala Phe Tyr Cys Ile Leu Glu Pro Arg Ser Gly 
            180                 185                 190         


Asn His Cys Pro Ala Gly Asn Ser Tyr Thr Ser Phe Ala Thr Tyr His 
        195                 200                 205             


Thr Pro Ala Thr Asp Cys Ser Asp Gly Asn Tyr Asn Arg Asn Ala Ser 
    210                 215                 220                 


Leu Asn Ser Phe Lys Glu Tyr Phe Asn Leu Arg Asn Cys Thr Phe Met 
225                 230                 235                 240 


Tyr Thr Tyr Asn Ile Thr Glu Asp Glu Ile Leu Glu Trp Phe Gly Ile 
                245                 250                 255     


Thr Gln Thr Ala Gln Gly Val His Leu Phe Ser Ser Arg Tyr Val Asp 
            260                 265                 270         


Leu Tyr Gly Gly Asn Met Phe Gln Phe Ala Thr Leu Pro Val Tyr Asp 
        275                 280                 285             


Thr Ile Lys Tyr Tyr Ser Ile Ile Pro His Ser Ile Arg Ser Ile Gln 
    290                 295                 300                 


Ser Asp Arg Lys Ala Trp Ala Ala Phe Tyr Val Tyr Lys Leu Gln Pro 
305                 310                 315                 320 


Leu Thr Phe Leu Leu Asp Phe Ser Val Asp Gly Tyr Ile Arg Arg Ala 
                325                 330                 335     


Ile Asp Cys Gly Phe Asn Asp Leu Ser Gln Leu His Cys Ser Tyr Glu 
            340                 345                 350         


Ser Phe Asp Val Glu Ser Gly Val Tyr Ser Val Ser Ser Phe Glu Ala 
        355                 360                 365             


Lys Pro Ser Gly Ser Val Val Glu Gln Ala Glu Gly Val Glu Cys Asp 
    370                 375                 380                 


Phe Ser Pro Leu Leu Ser Gly Thr Pro Pro Gln Val Tyr Asn Phe Lys 
385                 390                 395                 400 


Arg Leu Val Phe Thr Asn Cys Asn Tyr Asn Leu Thr Lys Leu Leu Ser 
                405                 410                 415     


Leu Phe Ser Val Asn Asp Phe Thr Cys Ser Gln Ile Ser Pro Ala Ala 
            420                 425                 430         


Ile Ala Ser Asn Cys Tyr Ser Ser Leu Ile Leu Asp Tyr Phe Ser Tyr 
        435                 440                 445             


Pro Leu Ser Met Lys Ser Asp Leu Ser Val Ser Ser Ala Gly Pro Ile 
    450                 455                 460                 


Ser Gln Phe Asn Tyr Lys Gln Ser Phe Ser Asn Pro Thr Cys Leu Ile 
465                 470                 475                 480 


Leu Ala Thr Val Pro His Asn Leu Thr Thr Ile Thr Lys Pro Leu Lys 
                485                 490                 495     


Tyr Ser Tyr Ile Asn Lys Cys Ser Arg Phe Leu Ser Asp Asp Arg Thr 
            500                 505                 510         


Glu Val Pro Gln Leu Val Asn Ala Asn Gln Tyr Ser Pro Cys Val Ser 
        515                 520                 525             


Ile Val Pro Ser Thr Val Trp Glu Asp Gly Asp Tyr Tyr Arg Lys Gln 
    530                 535                 540                 


Leu Ser Pro Leu Glu Gly Gly Gly Trp Leu Val Ala Ser Gly Ser Thr 
545                 550                 555                 560 


Val Ala Met Thr Glu Gln Leu Gln Met Gly Phe Gly Ile Thr Val Gln 
                565                 570                 575     


Tyr Gly Thr Asp Thr Asn Ser Val Cys Pro Lys Leu Glu Phe Ala Asn 
            580                 585                 590         


Asp Thr Lys Ile Ala Ser Gln Leu Gly Asn Cys Val Glu Tyr Ser Leu 
        595                 600                 605             


Tyr Gly Val Ser Gly Arg Gly Val Phe Gln Asn Cys Thr Ala Val Gly 
    610                 615                 620                 


Val Arg Gln Gln Arg Phe Val Tyr Asp Ala Tyr Gln Asn Leu Val Gly 
625                 630                 635                 640 


Tyr Tyr Ser Asp Asp Gly Asn Tyr Tyr Cys Leu Arg Ala Cys Val Ser 
                645                 650                 655     


Val Pro Val Ser Val Ile Tyr Asp Lys Glu Thr Lys Thr His Ala Thr 
            660                 665                 670         


Leu Phe Gly Ser Val Ala Cys Glu His Ile Ser Ser Thr Met Ser Gln 
        675                 680                 685             


Tyr Ser Arg Ser Thr Arg Ser Met Leu Lys Arg Arg Asp Ser Thr Tyr 
    690                 695                 700                 


Gly Pro Leu Gln Thr Pro Val Gly Cys Val Leu Gly Leu Val Asn Ser 
705                 710                 715                 720 


Ser Leu Phe Val Glu Asp Cys Lys Leu Pro Leu Gly Gln Ser Leu Cys 
                725                 730                 735     


Ala Leu Pro Asp Thr Pro Ser Thr Leu Thr Pro Arg Ser Val Arg Ser 
            740                 745                 750         


Val Pro Gly Glu Met Arg Leu Ala Ser Ile Ala Phe Asn His Pro Ile 
        755                 760                 765             


Gln Val Asp Gln Leu Asn Ser Ser Tyr Phe Lys Leu Ser Ile Pro Thr 
    770                 775                 780                 


Asn Phe Ser Phe Gly Val Thr Gln Glu Tyr Ile Gln Thr Thr Ile Gln 
785                 790                 795                 800 


Lys Val Thr Val Asp Cys Lys Gln Tyr Val Cys Asn Gly Phe Gln Lys 
                805                 810                 815     


Cys Glu Gln Leu Leu Arg Glu Tyr Gly Gln Phe Cys Ser Lys Ile Asn 
            820                 825                 830         


Gln Ala Leu His Gly Ala Asn Leu Arg Gln Asp Asp Ser Val Arg Asn 
        835                 840                 845             


Leu Phe Ala Ser Val Lys Ser Ser Gln Ser Ser Pro Ile Ile Pro Gly 
    850                 855                 860                 


Phe Gly Gly Asp Phe Asn Leu Thr Leu Leu Glu Pro Val Ser Ile Ser 
865                 870                 875                 880 


Thr Gly Ser Arg Ser Ala Arg Ser Ala Ile Glu Asp Leu Leu Phe Asp 
                885                 890                 895     


Lys Val Thr Ile Ala Asp Pro Gly Tyr Met Gln Gly Tyr Asp Asp Cys 
            900                 905                 910         


Met Gln Gln Gly Pro Ala Ser Ala Arg Asp Leu Ile Cys Ala Gln Tyr 
        915                 920                 925             


Val Ala Gly Tyr Lys Val Leu Pro Pro Leu Met Asp Val Asn Met Glu 
    930                 935                 940                 


Ala Ala Tyr Thr Ser Ser Leu Leu Gly Ser Ile Ala Gly Val Gly Trp 
945                 950                 955                 960 


Thr Ala Gly Leu Ser Ser Phe Ala Ala Ile Pro Phe Ala Gln Ser Ile 
                965                 970                 975     


Phe Tyr Arg Leu Asn Gly Val Gly Ile Thr Gln Gln Val Leu Ser Glu 
            980                 985                 990         


Asn Gln Lys Leu Ile Ala Asn Lys  Phe Asn Gln Ala Leu  Gly Ala Met 
        995                 1000                 1005             


Gln Thr  Gly Phe Thr Thr Thr  Asn Glu Ala Phe His  Lys Val Gln 
    1010                 1015                 1020             


Asp Ala  Val Asn Asn Asn Ala  Gln Ala Leu Ser Lys  Leu Ala Ser 
    1025                 1030                 1035             


Glu Leu  Ser Asn Thr Phe Gly  Ala Ile Ser Ala Ser  Ile Gly Asp 
    1040                 1045                 1050             


Ile Ile  Gln Arg Leu Asp Val  Leu Glu Gln Asp Ala  Gln Ile Asp 
    1055                 1060                 1065             


Arg Leu  Ile Asn Gly Arg Leu  Thr Thr Leu Asn Ala  Phe Val Ala 
    1070                 1075                 1080             


Gln Gln  Leu Val Arg Ser Glu  Ser Ala Ala Leu Ser  Ala Gln Leu 
    1085                 1090                 1095             


Ala Lys  Asp Lys Val Asn Glu  Cys Val Lys Ala Gln  Ser Lys Arg 
    1100                 1105                 1110             


Ser Gly  Phe Cys Gly Gln Gly  Thr His Ile Val Ser  Phe Val Val 
    1115                 1120                 1125             


Asn Ala  Pro Asn Gly Leu Tyr  Phe Met His Val Gly  Tyr Tyr Pro 
    1130                 1135                 1140             


Ser Asn  His Ile Glu Val Val  Ser Ala Tyr Gly Leu  Cys Asp Ala 
    1145                 1150                 1155             


Ala Asn  Pro Thr Asn Cys Ile  Ala Pro Val Asn Gly  Tyr Phe Ile 
    1160                 1165                 1170             


Lys Thr  Asn Asn Thr Arg Ile  Val Asp Glu Trp Ser  Tyr Thr Gly 
    1175                 1180                 1185             


Ser Ser  Phe Tyr Ala Pro Glu  Pro Ile Thr Ser Leu  Asn Thr Lys 
    1190                 1195                 1200             


Tyr Val  Ala Pro Gln Val Thr  Tyr Gln Asn Ile Ser  Thr Asn Leu 
    1205                 1210                 1215             


Pro Pro  Pro Leu Leu Gly Asn  Ser Thr Gly Ile Asp  Phe Gln Asp 
    1220                 1225                 1230             


Glu Leu  Asp Glu Phe Phe Lys  Asn Val Ser Thr Ser  Ile Pro Asn 
    1235                 1240                 1245             


Phe Gly  Ser Leu Thr Gln Ile  Asn Thr Thr Leu Leu  Asp Leu Thr 
    1250                 1255                 1260             


Tyr Glu  Met Leu Ser Leu Gln  Gln Val Val Lys Ala  Leu Asn Glu 
    1265                 1270                 1275             


Ser Tyr  Ile Asp Leu Lys Glu  Leu Gly Asn Tyr Thr  Tyr Tyr Asn 
    1280                 1285                 1290             


Lys Trp  Pro Trp Tyr Ile Trp  Leu Gly Phe Ile Ala  Gly Leu Val 
    1295                 1300                 1305             


Ala Leu  Ala Leu Cys Val Phe  Phe Ile Leu Cys Cys  Thr Gly Cys 
    1310                 1315                 1320             


Gly Thr  Asn Cys Met Gly Lys  Leu Lys Cys Asn Arg  Cys Cys Asp 
    1325                 1330                 1335             


Arg Tyr  Glu Glu Tyr Asp Leu  Glu Pro His Lys Val  His Val His 
    1340                 1345                 1350             


<210>  2
<211>  1291
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  2

Met Ile His Ser Val Phe Leu Leu Met Phe Leu Leu Thr Pro Thr Glu 
1               5                   10                  15      


Ser Tyr Val Asp Val Gly Pro Asp Ser Val Lys Ser Ala Cys Ile Glu 
            20                  25                  30          


Val Asp Ile Gln Gln Thr Phe Phe Asp Lys Thr Trp Pro Arg Pro Ile 
        35                  40                  45              


Asp Val Ser Lys Ala Asp Gly Ile Ile Tyr Pro Gln Gly Arg Thr Tyr 
    50                  55                  60                  


Ser Asn Ile Thr Ile Thr Tyr Gln Gly Leu Phe Pro Tyr Gln Gly Asp 
65                  70                  75                  80  


His Gly Asp Met Tyr Val Tyr Ser Ala Gly His Ala Thr Gly Thr Thr 
                85                  90                  95      


Pro Gln Lys Leu Phe Val Ala Asn Tyr Ser Gln Asp Val Lys Gln Phe 
            100                 105                 110         


Ala Asn Gly Phe Val Val Arg Ile Gly Ala Ala Ala Asn Ser Thr Gly 
        115                 120                 125             


Thr Val Ile Ile Ser Pro Ser Thr Ser Ala Thr Ile Arg Lys Ile Tyr 
    130                 135                 140                 


Pro Ala Phe Met Leu Gly Ser Ser Val Gly Asn Phe Ser Asp Gly Lys 
145                 150                 155                 160 


Met Gly Arg Phe Phe Asn His Thr Leu Val Leu Leu Pro Asp Gly Cys 
                165                 170                 175     


Gly Thr Leu Leu Arg Ala Phe Tyr Cys Ile Leu Glu Pro Arg Ser Gly 
            180                 185                 190         


Asn His Cys Pro Ala Gly Asn Ser Tyr Thr Ser Phe Ala Thr Tyr His 
        195                 200                 205             


Thr Pro Ala Thr Asp Cys Ser Asp Gly Asn Tyr Asn Arg Asn Ala Ser 
    210                 215                 220                 


Leu Asn Ser Phe Lys Glu Tyr Phe Asn Leu Arg Asn Cys Thr Phe Met 
225                 230                 235                 240 


Tyr Thr Tyr Asn Ile Thr Glu Asp Glu Ile Leu Glu Trp Phe Gly Ile 
                245                 250                 255     


Thr Gln Thr Ala Gln Gly Val His Leu Phe Ser Ser Arg Tyr Val Asp 
            260                 265                 270         


Leu Tyr Gly Gly Asn Met Phe Gln Phe Ala Thr Leu Pro Val Tyr Asp 
        275                 280                 285             


Thr Ile Lys Tyr Tyr Ser Ile Ile Pro His Ser Ile Arg Ser Ile Gln 
    290                 295                 300                 


Ser Asp Arg Lys Ala Trp Ala Ala Phe Tyr Val Tyr Lys Leu Gln Pro 
305                 310                 315                 320 


Leu Thr Phe Leu Leu Asp Phe Ser Val Asp Gly Tyr Ile Arg Arg Ala 
                325                 330                 335     


Ile Asp Cys Gly Phe Asn Asp Leu Ser Gln Leu His Cys Ser Tyr Glu 
            340                 345                 350         


Ser Phe Asp Val Glu Ser Gly Val Tyr Ser Val Ser Ser Phe Glu Ala 
        355                 360                 365             


Lys Pro Ser Gly Ser Val Val Glu Gln Ala Glu Gly Val Glu Cys Asp 
    370                 375                 380                 


Phe Ser Pro Leu Leu Ser Gly Thr Pro Pro Gln Val Tyr Asn Phe Lys 
385                 390                 395                 400 


Arg Leu Val Phe Thr Asn Cys Asn Tyr Asn Leu Thr Lys Leu Leu Ser 
                405                 410                 415     


Leu Phe Ser Val Asn Asp Phe Thr Cys Ser Gln Ile Ser Pro Ala Ala 
            420                 425                 430         


Ile Ala Ser Asn Cys Tyr Ser Ser Leu Ile Leu Asp Tyr Phe Ser Tyr 
        435                 440                 445             


Pro Leu Ser Met Lys Ser Asp Leu Ser Val Ser Ser Ala Gly Pro Ile 
    450                 455                 460                 


Ser Gln Phe Asn Tyr Lys Gln Ser Phe Ser Asn Pro Thr Cys Leu Ile 
465                 470                 475                 480 


Leu Ala Thr Val Pro His Asn Leu Thr Thr Ile Thr Lys Pro Leu Lys 
                485                 490                 495     


Tyr Ser Tyr Ile Asn Lys Cys Ser Arg Phe Leu Ser Asp Asp Arg Thr 
            500                 505                 510         


Glu Val Pro Gln Leu Val Asn Ala Asn Gln Tyr Ser Pro Cys Val Ser 
        515                 520                 525             


Ile Val Pro Ser Thr Val Trp Glu Asp Gly Asp Tyr Tyr Arg Lys Gln 
    530                 535                 540                 


Leu Ser Pro Leu Glu Gly Gly Gly Trp Leu Val Ala Ser Gly Ser Thr 
545                 550                 555                 560 


Val Ala Met Thr Glu Gln Leu Gln Met Gly Phe Gly Ile Thr Val Gln 
                565                 570                 575     


Tyr Gly Thr Asp Thr Asn Ser Val Cys Pro Lys Leu Glu Phe Ala Asn 
            580                 585                 590         


Asp Thr Lys Ile Ala Ser Gln Leu Gly Asn Cys Val Glu Tyr Ser Leu 
        595                 600                 605             


Tyr Gly Val Ser Gly Arg Gly Val Phe Gln Asn Cys Thr Ala Val Gly 
    610                 615                 620                 


Val Arg Gln Gln Arg Phe Val Tyr Asp Ala Tyr Gln Asn Leu Val Gly 
625                 630                 635                 640 


Tyr Tyr Ser Asp Asp Gly Asn Tyr Tyr Cys Leu Arg Ala Cys Val Ser 
                645                 650                 655     


Val Pro Val Ser Val Ile Tyr Asp Lys Glu Thr Lys Thr His Ala Thr 
            660                 665                 670         


Leu Phe Gly Ser Val Ala Cys Glu His Ile Ser Ser Thr Met Ser Gln 
        675                 680                 685             


Tyr Ser Arg Ser Thr Arg Ser Met Leu Lys Arg Arg Asp Ser Thr Tyr 
    690                 695                 700                 


Gly Pro Leu Gln Thr Pro Val Gly Cys Val Leu Gly Leu Val Asn Ser 
705                 710                 715                 720 


Ser Leu Phe Val Glu Asp Cys Lys Leu Pro Leu Gly Gln Ser Leu Cys 
                725                 730                 735     


Ala Leu Pro Asp Thr Pro Ser Thr Leu Thr Pro Arg Ser Val Arg Ser 
            740                 745                 750         


Val Pro Gly Glu Met Arg Leu Ala Ser Ile Ala Phe Asn His Pro Ile 
        755                 760                 765             


Gln Val Asp Gln Leu Asn Ser Ser Tyr Phe Lys Leu Ser Ile Pro Thr 
    770                 775                 780                 


Asn Phe Ser Phe Gly Val Thr Gln Glu Tyr Ile Gln Thr Thr Ile Gln 
785                 790                 795                 800 


Lys Val Thr Val Asp Cys Lys Gln Tyr Val Cys Asn Gly Phe Gln Lys 
                805                 810                 815     


Cys Glu Gln Leu Leu Arg Glu Tyr Gly Gln Phe Cys Ser Lys Ile Asn 
            820                 825                 830         


Gln Ala Leu His Gly Ala Asn Leu Arg Gln Asp Asp Ser Val Arg Asn 
        835                 840                 845             


Leu Phe Ala Ser Val Lys Ser Ser Gln Ser Ser Pro Ile Ile Pro Gly 
    850                 855                 860                 


Phe Gly Gly Asp Phe Asn Leu Thr Leu Leu Glu Pro Val Ser Ile Ser 
865                 870                 875                 880 


Thr Gly Ser Arg Ser Ala Arg Ser Ala Ile Glu Asp Leu Leu Phe Asp 
                885                 890                 895     


Lys Val Thr Ile Ala Asp Pro Gly Tyr Met Gln Gly Tyr Asp Asp Cys 
            900                 905                 910         


Met Gln Gln Gly Pro Ala Ser Ala Arg Asp Leu Ile Cys Ala Gln Tyr 
        915                 920                 925             


Val Ala Gly Tyr Lys Val Leu Pro Pro Leu Met Asp Val Asn Met Glu 
    930                 935                 940                 


Ala Ala Tyr Thr Ser Ser Leu Leu Gly Ser Ile Ala Gly Val Gly Trp 
945                 950                 955                 960 


Thr Ala Gly Leu Ser Ser Phe Ala Ala Ile Pro Phe Ala Gln Ser Ile 
                965                 970                 975     


Phe Tyr Arg Leu Asn Gly Val Gly Ile Thr Gln Gln Val Leu Ser Glu 
            980                 985                 990         


Asn Gln Lys Leu Ile Ala Asn Lys  Phe Asn Gln Ala Leu  Gly Ala Met 
        995                 1000                 1005             


Gln Thr  Gly Phe Thr Thr Thr  Asn Glu Ala Phe His  Lys Val Gln 
    1010                 1015                 1020             


Asp Ala  Val Asn Asn Asn Ala  Gln Ala Leu Ser Lys  Leu Ala Ser 
    1025                 1030                 1035             


Glu Leu  Ser Asn Thr Phe Gly  Ala Ile Ser Ala Ser  Ile Gly Asp 
    1040                 1045                 1050             


Ile Ile  Gln Arg Leu Asp Pro  Pro Glu Gln Asp Ala  Gln Ile Asp 
    1055                 1060                 1065             


Arg Leu  Ile Asn Gly Arg Leu  Thr Thr Leu Asn Ala  Phe Val Ala 
    1070                 1075                 1080             


Gln Gln  Leu Val Arg Ser Glu  Ser Ala Ala Leu Ser  Ala Gln Leu 
    1085                 1090                 1095             


Ala Lys  Asp Lys Val Asn Glu  Cys Val Lys Ala Gln  Ser Lys Arg 
    1100                 1105                 1110             


Ser Gly  Phe Cys Gly Gln Gly  Thr His Ile Val Ser  Phe Val Val 
    1115                 1120                 1125             


Asn Ala  Pro Asn Gly Leu Tyr  Phe Met His Val Gly  Tyr Tyr Pro 
    1130                 1135                 1140             


Ser Asn  His Ile Glu Val Val  Ser Ala Tyr Gly Leu  Cys Asp Ala 
    1145                 1150                 1155             


Ala Asn  Pro Thr Asn Cys Ile  Ala Pro Val Asn Gly  Tyr Phe Ile 
    1160                 1165                 1170             


Lys Thr  Asn Asn Thr Arg Ile  Val Asp Glu Trp Ser  Tyr Thr Gly 
    1175                 1180                 1185             


Ser Ser  Phe Tyr Ala Pro Glu  Pro Ile Thr Ser Leu  Asn Thr Lys 
    1190                 1195                 1200             


Tyr Val  Ala Pro Gln Val Thr  Tyr Gln Asn Ile Ser  Thr Asn Leu 
    1205                 1210                 1215             


Pro Pro  Pro Leu Leu Gly Asn  Ser Thr Gly Ile Asp  Phe Gln Asp 
    1220                 1225                 1230             


Glu Leu  Asp Glu Phe Phe Lys  Asn Val Ser Thr Ser  Ile Pro Asn 
    1235                 1240                 1245             


Phe Gly  Ser Leu Thr Gln Ile  Asn Thr Thr Leu Leu  Asp Leu Thr 
    1250                 1255                 1260             


Tyr Glu  Met Leu Ser Leu Gln  Gln Val Val Lys Ala  Leu Asn Glu 
    1265                 1270                 1275             


Ser Tyr  Ile Asp Leu Lys Glu  Leu Gly Asn Tyr Thr  Tyr 
    1280                 1285                 1290     


<210>  3
<211>  1291
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  3

Met Ile His Ser Val Phe Leu Leu Met Phe Leu Leu Thr Pro Thr Glu 
1               5                   10                  15      


Ser Tyr Val Asp Val Gly Pro Asp Ser Val Lys Ser Ala Cys Ile Glu 
            20                  25                  30          


Val Asp Ile Gln Gln Thr Phe Phe Asp Lys Thr Trp Pro Arg Pro Ile 
        35                  40                  45              


Asp Val Ser Lys Ala Asp Gly Ile Ile Tyr Pro Gln Gly Arg Thr Tyr 
    50                  55                  60                  


Ser Asn Ile Thr Ile Thr Tyr Gln Gly Leu Phe Pro Tyr Gln Gly Asp 
65                  70                  75                  80  


His Gly Asp Met Tyr Val Tyr Ser Ala Gly His Ala Thr Gly Thr Thr 
                85                  90                  95      


Pro Gln Lys Leu Phe Val Ala Asn Tyr Ser Gln Asp Val Lys Gln Phe 
            100                 105                 110         


Ala Asn Gly Phe Val Val Arg Ile Gly Ala Ala Ala Asn Ser Thr Gly 
        115                 120                 125             


Thr Val Ile Ile Ser Pro Ser Thr Ser Ala Thr Ile Arg Lys Ile Tyr 
    130                 135                 140                 


Pro Ala Phe Met Leu Gly Ser Ser Val Gly Asn Phe Ser Asp Gly Lys 
145                 150                 155                 160 


Met Gly Arg Phe Phe Asn His Thr Leu Val Leu Leu Pro Asp Gly Cys 
                165                 170                 175     


Gly Thr Leu Leu Arg Ala Phe Tyr Cys Ile Leu Glu Pro Arg Ser Gly 
            180                 185                 190         


Asn His Cys Pro Ala Gly Asn Ser Tyr Thr Ser Phe Ala Thr Tyr His 
        195                 200                 205             


Thr Pro Ala Thr Asp Cys Ser Asp Gly Asn Tyr Asn Arg Asn Ala Ser 
    210                 215                 220                 


Leu Asn Ser Phe Lys Glu Tyr Phe Asn Leu Arg Asn Cys Thr Phe Met 
225                 230                 235                 240 


Tyr Thr Tyr Asn Ile Thr Glu Asp Glu Ile Leu Glu Trp Phe Gly Ile 
                245                 250                 255     


Thr Gln Thr Ala Gln Gly Val His Leu Phe Ser Ser Arg Tyr Val Asp 
            260                 265                 270         


Leu Tyr Gly Gly Asn Met Phe Gln Phe Ala Thr Leu Pro Val Tyr Asp 
        275                 280                 285             


Thr Ile Lys Tyr Tyr Ser Ile Ile Pro His Ser Ile Arg Ser Ile Gln 
    290                 295                 300                 


Ser Asp Arg Lys Ala Trp Ala Ala Phe Tyr Val Tyr Lys Leu Gln Pro 
305                 310                 315                 320 


Leu Thr Phe Leu Leu Asp Phe Ser Val Asp Gly Tyr Ile Arg Arg Ala 
                325                 330                 335     


Ile Asp Cys Gly Phe Asn Asp Leu Ser Gln Leu His Cys Ser Tyr Glu 
            340                 345                 350         


Ser Phe Asp Val Glu Ser Gly Val Tyr Ser Val Ser Ser Phe Glu Ala 
        355                 360                 365             


Lys Pro Ser Gly Ser Val Val Glu Gln Ala Glu Gly Val Glu Cys Asp 
    370                 375                 380                 


Phe Ser Pro Leu Leu Ser Gly Thr Pro Pro Gln Val Tyr Asn Phe Lys 
385                 390                 395                 400 


Arg Leu Val Phe Thr Asn Cys Asn Tyr Asn Leu Thr Lys Leu Leu Ser 
                405                 410                 415     


Leu Phe Ser Val Asn Asp Phe Thr Cys Ser Gln Ile Ser Pro Ala Ala 
            420                 425                 430         


Ile Ala Ser Asn Cys Tyr Ser Ser Leu Ile Leu Asp Tyr Phe Ser Tyr 
        435                 440                 445             


Pro Leu Ser Met Lys Ser Asp Leu Ser Val Ser Ser Ala Gly Pro Ile 
    450                 455                 460                 


Ser Gln Phe Asn Tyr Lys Gln Ser Phe Ser Asn Pro Thr Cys Leu Ile 
465                 470                 475                 480 


Leu Ala Thr Val Pro His Asn Leu Thr Thr Ile Thr Lys Pro Leu Lys 
                485                 490                 495     


Tyr Ser Tyr Ile Asn Lys Cys Ser Arg Phe Leu Ser Asp Asp Arg Thr 
            500                 505                 510         


Glu Val Pro Gln Leu Val Asn Ala Asn Gln Tyr Ser Pro Cys Val Ser 
        515                 520                 525             


Ile Val Pro Ser Thr Val Trp Glu Asp Gly Asp Tyr Tyr Arg Lys Gln 
    530                 535                 540                 


Leu Ser Pro Leu Glu Gly Gly Gly Trp Leu Val Ala Ser Gly Ser Thr 
545                 550                 555                 560 


Val Ala Met Thr Glu Gln Leu Gln Met Gly Phe Gly Ile Thr Val Gln 
                565                 570                 575     


Tyr Gly Thr Asp Thr Asn Ser Val Cys Pro Lys Leu Glu Phe Ala Asn 
            580                 585                 590         


Asp Thr Lys Ile Ala Ser Gln Leu Gly Asn Cys Val Glu Tyr Ser Leu 
        595                 600                 605             


Tyr Gly Val Ser Gly Arg Gly Val Phe Gln Asn Cys Thr Ala Val Gly 
    610                 615                 620                 


Val Arg Gln Gln Arg Phe Val Tyr Asp Ala Tyr Gln Asn Leu Val Gly 
625                 630                 635                 640 


Tyr Tyr Ser Asp Asp Gly Asn Tyr Tyr Cys Leu Arg Ala Cys Val Ser 
                645                 650                 655     


Val Pro Val Ser Val Ile Tyr Asp Lys Glu Thr Lys Thr His Ala Thr 
            660                 665                 670         


Leu Phe Gly Ser Val Ala Cys Glu His Ile Ser Ser Thr Met Ser Gln 
        675                 680                 685             


Tyr Ser Arg Ser Thr Arg Ser Met Leu Lys Arg Arg Asp Ser Thr Tyr 
    690                 695                 700                 


Gly Pro Leu Gln Thr Pro Val Gly Cys Val Leu Gly Leu Val Asn Ser 
705                 710                 715                 720 


Ser Leu Phe Val Glu Asp Cys Lys Leu Pro Leu Gly Gln Ser Leu Cys 
                725                 730                 735     


Ala Leu Pro Asp Thr Pro Ser Thr Leu Thr Pro Ala Ser Val Gly Ser 
            740                 745                 750         


Val Pro Gly Glu Met Arg Leu Ala Ser Ile Ala Phe Asn His Pro Ile 
        755                 760                 765             


Gln Val Asp Gln Leu Asn Ser Ser Tyr Phe Lys Leu Ser Ile Pro Thr 
    770                 775                 780                 


Asn Phe Ser Phe Gly Val Thr Gln Glu Tyr Ile Gln Thr Thr Ile Gln 
785                 790                 795                 800 


Lys Val Thr Val Asp Cys Lys Gln Tyr Val Cys Asn Gly Phe Gln Lys 
                805                 810                 815     


Cys Glu Gln Leu Leu Arg Glu Tyr Gly Gln Phe Cys Ser Lys Ile Asn 
            820                 825                 830         


Gln Ala Leu His Gly Ala Asn Leu Arg Gln Asp Asp Ser Val Arg Asn 
        835                 840                 845             


Leu Phe Ala Ser Val Lys Ser Ser Gln Ser Ser Pro Ile Ile Pro Gly 
    850                 855                 860                 


Phe Gly Gly Asp Phe Asn Leu Thr Leu Leu Glu Pro Val Ser Ile Ser 
865                 870                 875                 880 


Thr Gly Ser Arg Ser Ala Arg Ser Ala Ile Glu Asp Leu Leu Phe Asp 
                885                 890                 895     


Lys Val Thr Ile Ala Asp Pro Gly Tyr Met Gln Gly Tyr Asp Asp Cys 
            900                 905                 910         


Met Gln Gln Gly Pro Ala Ser Ala Arg Asp Leu Ile Cys Ala Gln Tyr 
        915                 920                 925             


Val Ala Gly Tyr Lys Val Leu Pro Pro Leu Met Asp Val Asn Met Glu 
    930                 935                 940                 


Ala Ala Tyr Thr Ser Ser Leu Leu Gly Ser Ile Ala Gly Val Gly Trp 
945                 950                 955                 960 


Thr Ala Gly Leu Ser Ser Phe Ala Ala Ile Pro Phe Ala Gln Ser Ile 
                965                 970                 975     


Phe Tyr Arg Leu Asn Gly Val Gly Ile Thr Gln Gln Val Leu Ser Glu 
            980                 985                 990         


Asn Gln Lys Leu Ile Ala Asn Lys  Phe Asn Gln Ala Leu  Gly Ala Met 
        995                 1000                 1005             


Gln Thr  Gly Phe Thr Thr Thr  Asn Glu Ala Phe His  Lys Val Gln 
    1010                 1015                 1020             


Asp Ala  Val Asn Asn Asn Ala  Gln Ala Leu Ser Lys  Leu Ala Ser 
    1025                 1030                 1035             


Glu Leu  Ser Asn Thr Phe Gly  Ala Ile Ser Ala Ser  Ile Gly Asp 
    1040                 1045                 1050             


Ile Ile  Gln Arg Leu Asp Pro  Pro Glu Gln Asp Ala  Gln Ile Asp 
    1055                 1060                 1065             


Arg Leu  Ile Asn Gly Arg Leu  Thr Thr Leu Asn Ala  Phe Val Ala 
    1070                 1075                 1080             


Gln Gln  Leu Val Arg Ser Glu  Ser Ala Ala Leu Ser  Ala Gln Leu 
    1085                 1090                 1095             


Ala Lys  Asp Lys Val Asn Glu  Cys Val Lys Ala Gln  Ser Lys Arg 
    1100                 1105                 1110             


Ser Gly  Phe Cys Gly Gln Gly  Thr His Ile Val Ser  Phe Val Val 
    1115                 1120                 1125             


Asn Ala  Pro Asn Gly Leu Tyr  Phe Met His Val Gly  Tyr Tyr Pro 
    1130                 1135                 1140             


Ser Asn  His Ile Glu Val Val  Ser Ala Tyr Gly Leu  Cys Asp Ala 
    1145                 1150                 1155             


Ala Asn  Pro Thr Asn Cys Ile  Ala Pro Val Asn Gly  Tyr Phe Ile 
    1160                 1165                 1170             


Lys Thr  Asn Asn Thr Arg Ile  Val Asp Glu Trp Ser  Tyr Thr Gly 
    1175                 1180                 1185             


Ser Ser  Phe Tyr Ala Pro Glu  Pro Ile Thr Ser Leu  Asn Thr Lys 
    1190                 1195                 1200             


Tyr Val  Ala Pro Gln Val Thr  Tyr Gln Asn Ile Ser  Thr Asn Leu 
    1205                 1210                 1215             


Pro Pro  Pro Leu Leu Gly Asn  Ser Thr Gly Ile Asp  Phe Gln Asp 
    1220                 1225                 1230             


Glu Leu  Asp Glu Phe Phe Lys  Asn Val Ser Thr Ser  Ile Pro Asn 
    1235                 1240                 1245             


Phe Gly  Ser Leu Thr Gln Ile  Asn Thr Thr Leu Leu  Asp Leu Thr 
    1250                 1255                 1260             


Tyr Glu  Met Leu Ser Leu Gln  Gln Val Val Lys Ala  Leu Asn Glu 
    1265                 1270                 1275             


Ser Tyr  Ile Asp Leu Lys Glu  Leu Gly Asn Tyr Thr  Tyr 
    1280                 1285                 1290     


<210>  4
<211>  1291
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  4

Met Ile His Ser Val Phe Leu Leu Met Phe Leu Leu Thr Pro Thr Glu 
1               5                   10                  15      


Ser Tyr Val Asp Val Gly Pro Asp Ser Val Lys Ser Ala Cys Ile Glu 
            20                  25                  30          


Val Asp Ile Gln Gln Thr Phe Phe Asp Lys Thr Trp Pro Arg Pro Ile 
        35                  40                  45              


Asp Val Ser Lys Ala Asp Gly Ile Ile Tyr Pro Gln Gly Arg Thr Tyr 
    50                  55                  60                  


Ser Asn Ile Thr Ile Thr Tyr Gln Gly Leu Phe Pro Tyr Gln Gly Asp 
65                  70                  75                  80  


His Gly Asp Met Tyr Val Tyr Ser Ala Gly His Ala Thr Gly Thr Thr 
                85                  90                  95      


Pro Gln Lys Leu Phe Val Ala Asn Tyr Ser Gln Asp Val Lys Gln Phe 
            100                 105                 110         


Ala Asn Gly Phe Val Val Arg Ile Gly Ala Ala Ala Asn Ser Thr Gly 
        115                 120                 125             


Thr Val Ile Ile Ser Pro Ser Thr Ser Ala Thr Ile Arg Lys Ile Tyr 
    130                 135                 140                 


Pro Ala Phe Met Leu Gly Ser Ser Val Gly Asn Phe Ser Asp Gly Lys 
145                 150                 155                 160 


Met Gly Arg Phe Phe Asn His Thr Leu Val Leu Leu Pro Asp Gly Cys 
                165                 170                 175     


Gly Thr Leu Leu Arg Ala Phe Tyr Cys Ile Leu Glu Pro Arg Ser Gly 
            180                 185                 190         


Asn His Cys Pro Ala Gly Asn Ser Tyr Thr Ser Phe Ala Thr Tyr His 
        195                 200                 205             


Thr Pro Ala Thr Asp Cys Ser Asp Gly Asn Tyr Asn Arg Asn Ala Ser 
    210                 215                 220                 


Leu Asn Ser Phe Lys Glu Tyr Phe Asn Leu Arg Asn Cys Thr Phe Met 
225                 230                 235                 240 


Tyr Thr Tyr Asn Ile Thr Glu Asp Glu Ile Leu Glu Trp Phe Gly Ile 
                245                 250                 255     


Thr Gln Thr Ala Gln Gly Val His Leu Phe Ser Ser Arg Tyr Val Asp 
            260                 265                 270         


Leu Tyr Gly Gly Asn Met Phe Gln Phe Ala Thr Leu Pro Val Tyr Asp 
        275                 280                 285             


Thr Ile Lys Tyr Tyr Ser Ile Ile Pro His Ser Ile Arg Ser Ile Gln 
    290                 295                 300                 


Ser Asp Arg Lys Ala Trp Ala Ala Phe Tyr Val Tyr Lys Leu Gln Pro 
305                 310                 315                 320 


Leu Thr Phe Leu Leu Asp Phe Ser Val Asp Gly Tyr Ile Arg Arg Ala 
                325                 330                 335     


Ile Asp Cys Gly Phe Asn Asp Leu Ser Gln Leu His Cys Ser Tyr Glu 
            340                 345                 350         


Ser Phe Asp Val Glu Ser Gly Val Tyr Ser Val Ser Ser Phe Glu Ala 
        355                 360                 365             


Lys Pro Ser Gly Ser Val Val Glu Gln Ala Glu Gly Val Glu Cys Asp 
    370                 375                 380                 


Phe Ser Pro Leu Leu Ser Gly Thr Pro Pro Gln Val Tyr Asn Phe Lys 
385                 390                 395                 400 


Arg Leu Val Phe Thr Asn Cys Asn Tyr Asn Leu Thr Lys Leu Leu Ser 
                405                 410                 415     


Leu Phe Ser Val Asn Asp Phe Thr Cys Ser Gln Ile Ser Pro Ala Ala 
            420                 425                 430         


Ile Ala Ser Asn Cys Tyr Ser Ser Leu Ile Leu Asp Tyr Phe Ser Tyr 
        435                 440                 445             


Pro Leu Ser Met Lys Ser Asp Leu Ser Val Ser Ser Ala Gly Pro Ile 
    450                 455                 460                 


Ser Gln Phe Asn Tyr Lys Gln Ser Phe Ser Asn Pro Thr Cys Leu Ile 
465                 470                 475                 480 


Leu Ala Thr Val Pro His Asn Leu Thr Thr Ile Thr Lys Pro Leu Lys 
                485                 490                 495     


Tyr Ser Tyr Ile Asn Lys Cys Ser Arg Phe Leu Ser Asp Asp Arg Thr 
            500                 505                 510         


Glu Val Pro Gln Leu Val Asn Ala Asn Gln Tyr Ser Pro Cys Val Ser 
        515                 520                 525             


Ile Val Pro Ser Thr Val Trp Glu Asp Gly Asp Tyr Tyr Arg Lys Gln 
    530                 535                 540                 


Leu Ser Pro Leu Glu Gly Gly Gly Trp Leu Val Ala Ser Gly Ser Thr 
545                 550                 555                 560 


Val Ala Met Thr Glu Gln Leu Gln Met Gly Phe Gly Ile Thr Val Gln 
                565                 570                 575     


Tyr Gly Thr Asp Thr Asn Ser Val Cys Pro Lys Leu Glu Phe Ala Asn 
            580                 585                 590         


Asp Thr Lys Ile Ala Ser Gln Leu Gly Asn Cys Val Glu Tyr Ser Leu 
        595                 600                 605             


Tyr Gly Val Ser Gly Arg Gly Val Phe Gln Asn Cys Thr Ala Val Gly 
    610                 615                 620                 


Val Arg Gln Gln Arg Phe Val Tyr Asp Ala Tyr Gln Asn Leu Val Gly 
625                 630                 635                 640 


Tyr Tyr Ser Asp Asp Gly Asn Tyr Tyr Cys Leu Arg Ala Cys Val Ser 
                645                 650                 655     


Val Pro Val Ser Val Ile Tyr Asp Lys Glu Thr Lys Thr His Ala Thr 
            660                 665                 670         


Leu Phe Gly Ser Val Ala Cys Glu His Ile Ser Ser Thr Met Ser Gln 
        675                 680                 685             


Tyr Ser Arg Ser Thr Arg Ser Met Leu Lys Arg Arg Asp Ser Thr Tyr 
    690                 695                 700                 


Gly Pro Leu Gln Thr Pro Val Gly Cys Val Leu Gly Leu Val Asn Ser 
705                 710                 715                 720 


Ser Leu Phe Val Glu Asp Cys Lys Leu Pro Leu Gly Gln Ser Leu Cys 
                725                 730                 735     


Ala Leu Pro Asp Thr Pro Ser Thr Leu Thr Pro Ala Ser Val Gly Ser 
            740                 745                 750         


Val Pro Gly Glu Met Arg Leu Ala Ser Ile Ala Phe Asn His Pro Ile 
        755                 760                 765             


Gln Val Asp Gln Leu Asn Ser Ser Tyr Phe Lys Leu Ser Ile Pro Thr 
    770                 775                 780                 


Asn Phe Ser Phe Gly Val Thr Gln Glu Tyr Ile Gln Thr Thr Ile Gln 
785                 790                 795                 800 


Lys Val Thr Val Asp Cys Lys Gln Tyr Val Cys Asn Gly Phe Gln Lys 
                805                 810                 815     


Cys Glu Gln Leu Leu Arg Glu Tyr Gly Gln Phe Cys Ser Lys Ile Asn 
            820                 825                 830         


Gln Ala Leu His Gly Ala Asn Leu Arg Gln Asp Asp Ser Val Arg Asn 
        835                 840                 845             


Leu Phe Ala Ser Val Lys Ser Ser Gln Ser Ser Pro Ile Ile Pro Gly 
    850                 855                 860                 


Phe Gly Gly Asp Phe Asn Leu Thr Leu Leu Glu Pro Val Ser Ile Ser 
865                 870                 875                 880 


Thr Gly Ser Gly Ser Ala Gly Ser Ala Ile Glu Asp Leu Leu Phe Asp 
                885                 890                 895     


Lys Val Thr Ile Ala Asp Pro Gly Tyr Met Gln Gly Tyr Asp Asp Cys 
            900                 905                 910         


Met Gln Gln Gly Pro Ala Ser Ala Arg Asp Leu Ile Cys Ala Gln Tyr 
        915                 920                 925             


Val Ala Gly Tyr Lys Val Leu Pro Pro Leu Met Asp Val Asn Met Glu 
    930                 935                 940                 


Ala Ala Tyr Thr Ser Ser Leu Leu Gly Ser Ile Ala Gly Val Gly Trp 
945                 950                 955                 960 


Thr Ala Gly Leu Ser Ser Phe Ala Ala Ile Pro Phe Ala Gln Ser Ile 
                965                 970                 975     


Phe Tyr Arg Leu Asn Gly Val Gly Ile Thr Gln Gln Val Leu Ser Glu 
            980                 985                 990         


Asn Gln Lys Leu Ile Ala Asn Lys  Phe Asn Gln Ala Leu  Gly Ala Met 
        995                 1000                 1005             


Gln Thr  Gly Phe Thr Thr Thr  Asn Glu Ala Phe His  Lys Val Gln 
    1010                 1015                 1020             


Asp Ala  Val Asn Asn Asn Ala  Gln Ala Leu Ser Lys  Leu Ala Ser 
    1025                 1030                 1035             


Glu Leu  Ser Asn Thr Phe Gly  Ala Ile Ser Ala Ser  Ile Gly Asp 
    1040                 1045                 1050             


Ile Ile  Gln Arg Leu Asp Pro  Pro Glu Gln Asp Ala  Gln Ile Asp 
    1055                 1060                 1065             


Arg Leu  Ile Asn Gly Arg Leu  Thr Thr Leu Asn Ala  Phe Val Ala 
    1070                 1075                 1080             


Gln Gln  Leu Val Arg Ser Glu  Ser Ala Ala Leu Ser  Ala Gln Leu 
    1085                 1090                 1095             


Ala Lys  Asp Lys Val Asn Glu  Cys Val Lys Ala Gln  Ser Lys Arg 
    1100                 1105                 1110             


Ser Gly  Phe Cys Gly Gln Gly  Thr His Ile Val Ser  Phe Val Val 
    1115                 1120                 1125             


Asn Ala  Pro Asn Gly Leu Tyr  Phe Met His Val Gly  Tyr Tyr Pro 
    1130                 1135                 1140             


Ser Asn  His Ile Glu Val Val  Ser Ala Tyr Gly Leu  Cys Asp Ala 
    1145                 1150                 1155             


Ala Asn  Pro Thr Asn Cys Ile  Ala Pro Val Asn Gly  Tyr Phe Ile 
    1160                 1165                 1170             


Lys Thr  Asn Asn Thr Arg Ile  Val Asp Glu Trp Ser  Tyr Thr Gly 
    1175                 1180                 1185             


Ser Ser  Phe Tyr Ala Pro Glu  Pro Ile Thr Ser Leu  Asn Thr Lys 
    1190                 1195                 1200             


Tyr Val  Ala Pro Gln Val Thr  Tyr Gln Asn Ile Ser  Thr Asn Leu 
    1205                 1210                 1215             


Pro Pro  Pro Leu Leu Gly Asn  Ser Thr Gly Ile Asp  Phe Gln Asp 
    1220                 1225                 1230             


Glu Leu  Asp Glu Phe Phe Lys  Asn Val Ser Thr Ser  Ile Pro Asn 
    1235                 1240                 1245             


Phe Gly  Ser Leu Thr Gln Ile  Asn Thr Thr Leu Leu  Asp Leu Thr 
    1250                 1255                 1260             


Tyr Glu  Met Leu Ser Leu Gln  Gln Val Val Lys Ala  Leu Asn Glu 
    1265                 1270                 1275             


Ser Tyr  Ile Asp Leu Lys Glu  Leu Gly Asn Tyr Thr  Tyr 
    1280                 1285                 1290     


<210>  5
<211>  4062
<212>  DNA
<213>  middle east respiratory syndrome coronavirus

<400>  5
atgattcact ccgtgttcct gctgatgttc ctgctgactc ctacagagag ctatgtggat       60

gtgggacctg attccgtcaa gagcgcctgc atcgaagtgg acattcagca gaccttcttt      120

gataagacat ggccaagacc catcgacgtg agcaaagccg atggcatcat ctaccctcag      180

gggaggacct attccaatat cacaattact taccagggcc tgttcccata tcagggagac      240

cacggcgata tgtacgtgta ttctgctggc catgcaacag ggaccacacc tcagaagctg      300

tttgtggcta actacagcca ggacgtcaaa cagttcgcaa atggatttgt ggtccgcatc      360

ggcgccgctg caaactctac cggcacagtg atcatttcac ctagcacttc cgcaaccatc      420

cgaaaaatct acccagcctt catgctggga agctccgtgg gcaattttag cgacgggaaa      480

atgggacggt tctttaacca caccctggtg ctgctgcctg atggatgcgg cacactgctg      540

agggctttct actgtatcct ggagccacgc agcggaaacc actgccccgc aggaaatagc      600

tacacctcct ttgccacata tcatactcca gctaccgact gttccgatgg caactacaat      660

cgaaacgcct ctctgaatag tttcaaggaa tacttcaacc tgcggaattg cacattcatg      720

tacacttata acatcaccga ggacgaaatt ctggagtggt tcggaatcac tcagaccgca      780

cagggcgtgc acctgttttc tagtcgctac gtcgacctgt atggcgggaa catgttccag      840

tttgccactc tgcccgtgta cgataccatc aagtactatt ccatcattcc tcattcaatc      900

cgcagcattc agtccgatcg aaaggcttgg gccgctttct acgtgtataa actgcagcca      960

ctgaccttcc tgctggactt tagcgtcgat ggctacatcc ggagagccat tgactgcggg     1020

tttaatgatc tgtcccagct gcactgttct tacgaaagtt tcgacgtgga gtccggcgtg     1080

tattctgtct caagctttga ggccaagccc tctgggagtg tggtcgagca ggctgaagga     1140

gtggagtgcg atttcagtcc tctgctgtca gggacccccc ctcaggtgta caacttcaag     1200

cggctggtct ttactaactg taactacaat ctgaccaagc tgctgtcact gttcagcgtg     1260

aatgacttta catgctccca gatcagcccc gcagccattg ctagtaactg ttactcctct     1320

ctgatcctgg actacttctc atatccactg agtatgaaga gcgacctgag cgtgagttca     1380

gccggcccca tcagccagtt caactataaa cagagcttca gcaatcctac atgcctgatt     1440

ctggctactg tgccacataa tctgactacc atcactaagc ccctgaaata ctcctatatt     1500

aacaagtgca gccggttcct gtccgacgat agaaccgaag tgccacagct ggtcaacgcc     1560

aatcagtact ctccctgtgt gagtatcgtc ccttcaaccg tgtgggaaga cggggattac     1620

tatagaaaac agctgagccc cctggaggga ggaggatggc tggtggcatc cggatctaca     1680

gtcgccatga ctgagcagct gcagatgggg ttcggaatca cagtgcagta cggcacagac     1740

actaactctg tctgtcccaa gctggaattc gctaacgata ctaagatcgc aagtcagctg     1800

ggaaactgcg tggagtactc tctgtatggc gtgagtggca gaggggtctt ccagaattgt     1860

accgcagtgg gcgtccgaca gcagcggttt gtgtacgacg cctatcagaa tctggtcggc     1920

tactatagcg acgatgggaa ctactattgc ctgagggcct gtgtgagcgt ccctgtgtcc     1980

gtcatctacg ataaggaaac caaaacacac gccacactgt tcgggtccgt ggcttgcgag     2040

catattagct ccacaatgtc tcagtacagt agatcaacta ggtcaatgct gaagaggcgc     2100

gatagcacct atggacctct gcagacacca gtggggtgtg tcctgggact ggtgaactct     2160

agtctgtttg tcgaggactg caagctgccc ctgggccaga gcctgtgcgc cctgcccgac     2220

acccccagca ccctgacccc ccggagcgtg cggagcgtgc ccggcgagat gcggctggcc     2280

agcatcgcct tcaaccaccc catccaggtg gaccagctga acagcagcta cttcaagctg     2340

agcatcccca ccaacttcag cttcggcgtg acccaggagt acatccagac caccatccag     2400

aaggtgaccg tggactgcaa gcagtacgtg tgcaacggct tccagaagtg cgagcagctg     2460

ctgcgggagt acggccagtt ctgcagcaag atcaaccagg ccctgcacgg cgccaacctg     2520

cggcaggacg acagcgtgcg gaacctgttc gccagcgtga agagcagcca gagcagcccc     2580

atcatccccg gcttcggcgg cgacttcaac ctgaccctgc tggagcccgt gagcatcagc     2640

accggcagcc ggagcgcccg gagcgccatc gaggacctgc tgttcgacaa ggtgaccatc     2700

gccgaccccg gctacatgca gggctacgac gactgcatgc agcagggccc cgccagcgcc     2760

cgggacctga tctgcgccca gtacgtggcc ggctacaagg tgctgccccc cctgatggac     2820

gtgaacatgg aggccgccta caccagcagc ctgctgggca gcatcgccgg cgtgggctgg     2880

accgccggcc tgagcagctt cgccgccatc cccttcgccc agagcatctt ctaccggctg     2940

aacggcgtgg gcatcaccca gcaggtgctg agcgagaacc agaagctgat cgccaacaag     3000

ttcaaccagg ccctgggcgc catgcagacc ggcttcacca ccaccaacga ggccttccac     3060

aaggtgcagg acgccgtgaa caacaacgcc caggccctga gcaagctggc cagcgagctg     3120

agcaacacct tcggcgccat cagcgccagc atcggcgaca tcatccagcg gctggacgtg     3180

ctggagcagg acgcccagat cgaccggctg atcaacggcc ggctgaccac cctgaacgcc     3240

ttcgtggccc agcagctggt gcggagcgag agcgccgccc tgagcgccca gctggccaag     3300

gacaaggtga acgagtgcgt gaaggcccag agcaagcgga gcggcttctg cggccagggc     3360

acccacatcg tgagcttcgt ggtgaacgcc cccaacggcc tgtacttcat gcacgtgggc     3420

tactacccca gcaaccacat cgaggtggtg agcgcctacg gcctgtgcga cgccgccaac     3480

cccaccaact gcatcgcccc cgtgaacggc tacttcatca agaccaacaa cacccggatc     3540

gtggacgagt ggagctacac cggcagcagc ttctacgccc ccgagcccat caccagcctg     3600

aacaccaagt acgtggcccc ccaggtgacc taccagaaca tcagcaccaa cctgcccccc     3660

cccctgctgg gcaacagcac cggcatcgac ttccaggacg agctggacga gttcttcaag     3720

aacgtgagca ccagcatccc caacttcggc agcctgaccc agatcaacac caccctgctg     3780

gacctgacct acgagatgct gagcctgcag caggtggtga aggccctgaa cgagagctac     3840

atcgacctga aggagctggg caactacacc tactacaaca agtggccctg gtacatctgg     3900

ctgggcttca tcgccggcct ggtggccctg gccctgtgcg tgttcttcat cctgtgctgc     3960

accggctgcg gcaccaactg catgggcaag ctgaagtgca accggtgctg cgaccggtac     4020

gaggagtacg acctggagcc ccacaaggtg cacgtgcact ga                        4062


<210>  6
<211>  1255
<212>  PRT
<213>  severe acute respiratory syndrome coronavirus

<400>  6

Met Phe Ile Phe Leu Leu Phe Leu Thr Leu Thr Ser Gly Ser Asp Leu 
1               5                   10                  15      


Asp Arg Cys Thr Thr Phe Asp Asp Val Gln Ala Pro Asn Tyr Thr Gln 
            20                  25                  30          


His Thr Ser Ser Met Arg Gly Val Tyr Tyr Pro Asp Glu Ile Phe Arg 
        35                  40                  45              


Ser Asp Thr Leu Tyr Leu Thr Gln Asp Leu Phe Leu Pro Phe Tyr Ser 
    50                  55                  60                  


Asn Val Thr Gly Phe His Thr Ile Asn His Thr Phe Gly Asn Pro Val 
65                  70                  75                  80  


Ile Pro Phe Lys Asp Gly Ile Tyr Phe Ala Ala Thr Glu Lys Ser Asn 
                85                  90                  95      


Val Val Arg Gly Trp Val Phe Gly Ser Thr Met Asn Asn Lys Ser Gln 
            100                 105                 110         


Ser Val Ile Ile Ile Asn Asn Ser Thr Asn Val Val Ile Arg Ala Cys 
        115                 120                 125             


Asn Phe Glu Leu Cys Asp Asn Pro Phe Phe Ala Val Ser Lys Pro Met 
    130                 135                 140                 


Gly Thr Gln Thr His Thr Met Ile Phe Asp Asn Ala Phe Asn Cys Thr 
145                 150                 155                 160 


Phe Glu Tyr Ile Ser Asp Ala Phe Ser Leu Asp Val Ser Glu Lys Ser 
                165                 170                 175     


Gly Asn Phe Lys His Leu Arg Glu Phe Val Phe Lys Asn Lys Asp Gly 
            180                 185                 190         


Phe Leu Tyr Val Tyr Lys Gly Tyr Gln Pro Ile Asp Val Val Arg Asp 
        195                 200                 205             


Leu Pro Ser Gly Phe Asn Thr Leu Lys Pro Ile Phe Lys Leu Pro Leu 
    210                 215                 220                 


Gly Ile Asn Ile Thr Asn Phe Arg Ala Ile Leu Thr Ala Phe Ser Pro 
225                 230                 235                 240 


Ala Gln Asp Ile Trp Gly Thr Ser Ala Ala Ala Tyr Phe Val Gly Tyr 
                245                 250                 255     


Leu Lys Pro Thr Thr Phe Met Leu Lys Tyr Asp Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ser Gln Asn Pro Leu Ala Glu Leu Lys Cys 
        275                 280                 285             


Ser Val Lys Ser Phe Glu Ile Asp Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Val Pro Ser Gly Asp Val Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Pro Ser 
                325                 330                 335     


Val Tyr Ala Trp Glu Arg Lys Lys Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Thr Phe Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Met Gly Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr Ser 
            420                 425                 430         


Thr Gly Asn Tyr Asn Tyr Lys Tyr Arg Tyr Leu Arg His Gly Lys Leu 
        435                 440                 445             


Arg Pro Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp Gly 
    450                 455                 460                 


Lys Pro Cys Thr Pro Pro Ala Leu Asn Cys Tyr Trp Pro Leu Asn Asp 
465                 470                 475                 480 


Tyr Gly Phe Tyr Thr Thr Thr Gly Ile Gly Tyr Gln Pro Tyr Arg Val 
                485                 490                 495     


Val Val Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys Gly 
            500                 505                 510         


Pro Lys Leu Ser Thr Asp Leu Ile Lys Asn Gln Cys Val Asn Phe Asn 
        515                 520                 525             


Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Pro Ser Ser Lys Arg 
    530                 535                 540                 


Phe Gln Pro Phe Gln Gln Phe Gly Arg Asp Val Ser Asp Phe Thr Asp 
545                 550                 555                 560 


Ser Val Arg Asp Pro Lys Thr Ser Glu Ile Leu Asp Ile Ser Pro Cys 
                565                 570                 575     


Ala Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Ala Ser Ser 
            580                 585                 590         


Glu Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Asp Val Ser Thr 
        595                 600                 605             


Ala Ile His Ala Asp Gln Leu Thr Pro Ala Trp Arg Ile Tyr Ser Thr 
    610                 615                 620                 


Gly Asn Asn Val Phe Gln Thr Gln Ala Gly Cys Leu Ile Gly Ala Glu 
625                 630                 635                 640 


His Val Asp Thr Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile 
                645                 650                 655     


Cys Ala Ser Tyr His Thr Val Ser Leu Leu Arg Ser Thr Ser Gln Lys 
            660                 665                 670         


Ser Ile Val Ala Tyr Thr Met Ser Leu Gly Ala Asp Ser Ser Ile Ala 
        675                 680                 685             


Tyr Ser Asn Asn Thr Ile Ala Ile Pro Thr Asn Phe Ser Ile Ser Ile 
    690                 695                 700                 


Thr Thr Glu Val Met Pro Val Ser Met Ala Lys Thr Ser Val Asp Cys 
705                 710                 715                 720 


Asn Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ala Asn Leu Leu Leu 
                725                 730                 735     


Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Ser Gly Ile 
            740                 745                 750         


Ala Ala Glu Gln Asp Arg Asn Thr Arg Glu Val Phe Ala Gln Val Lys 
        755                 760                 765             


Gln Met Tyr Lys Thr Pro Thr Leu Lys Tyr Phe Gly Gly Phe Asn Phe 
    770                 775                 780                 


Ser Gln Ile Leu Pro Asp Pro Leu Lys Pro Thr Lys Arg Ser Phe Ile 
785                 790                 795                 800 


Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Met 
                805                 810                 815     


Lys Gln Tyr Gly Glu Cys Leu Gly Asp Ile Asn Ala Arg Asp Leu Ile 
            820                 825                 830         


Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr 
        835                 840                 845             


Asp Asp Met Ile Ala Ala Tyr Thr Ala Ala Leu Val Ser Gly Thr Ala 
    850                 855                 860                 


Thr Ala Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe 
865                 870                 875                 880 


Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn 
                885                 890                 895     


Val Leu Tyr Glu Asn Gln Lys Gln Ile Ala Asn Gln Phe Asn Lys Ala 
            900                 905                 910         


Ile Ser Gln Ile Gln Glu Ser Leu Thr Thr Thr Ser Thr Ala Leu Gly 
        915                 920                 925             


Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu 
    930                 935                 940                 


Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn 
945                 950                 955                 960 


Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp 
                965                 970                 975     


Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln 
            980                 985                 990         


Gln Leu Ile Arg Ala Ala Glu Ile  Arg Ala Ser Ala Asn  Leu Ala Ala 
        995                 1000                 1005             


Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp 
    1010                 1015                 1020             


Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser Phe Pro  Gln Ala Ala 
    1025                 1030                 1035             


Pro His  Gly Val Val Phe Leu  His Val Thr Tyr Val  Pro Ser Gln 
    1040                 1045                 1050             


Glu Arg  Asn Phe Thr Thr Ala  Pro Ala Ile Cys His  Glu Gly Lys 
    1055                 1060                 1065             


Ala Tyr  Phe Pro Arg Glu Gly  Val Phe Val Phe Asn  Gly Thr Ser 
    1070                 1075                 1080             


Trp Phe  Ile Thr Gln Arg Asn  Phe Phe Ser Pro Gln  Ile Ile Thr 
    1085                 1090                 1095             


Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys Asp Val  Val Ile Gly 
    1100                 1105                 1110             


Ile Ile  Asn Asn Thr Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp 
    1115                 1120                 1125             


Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser 
    1130                 1135                 1140             


Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val 
    1145                 1150                 1155             


Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys 
    1160                 1165                 1170             


Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr 
    1175                 1180                 1185             


Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Val Trp Leu  Gly Phe Ile 
    1190                 1195                 1200             


Ala Gly  Leu Ile Ala Ile Val  Met Val Thr Ile Leu  Leu Cys Cys 
    1205                 1210                 1215             


Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly Ala Cys  Ser Cys Gly 
    1220                 1225                 1230             


Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser Glu Pro  Val Leu Lys 
    1235                 1240                 1245             


Gly Val  Lys Leu His Tyr Thr  
    1250                 1255 


<210>  7
<211>  1190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  7

Met Phe Ile Phe Leu Leu Phe Leu Thr Leu Thr Ser Gly Ser Asp Leu 
1               5                   10                  15      


Asp Arg Cys Thr Thr Phe Asp Asp Val Gln Ala Pro Asn Tyr Thr Gln 
            20                  25                  30          


His Thr Ser Ser Met Arg Gly Val Tyr Tyr Pro Asp Glu Ile Phe Arg 
        35                  40                  45              


Ser Asp Thr Leu Tyr Leu Thr Gln Asp Leu Phe Leu Pro Phe Tyr Ser 
    50                  55                  60                  


Asn Val Thr Gly Phe His Thr Ile Asn His Thr Phe Gly Asn Pro Val 
65                  70                  75                  80  


Ile Pro Phe Lys Asp Gly Ile Tyr Phe Ala Ala Thr Glu Lys Ser Asn 
                85                  90                  95      


Val Val Arg Gly Trp Val Phe Gly Ser Thr Met Asn Asn Lys Ser Gln 
            100                 105                 110         


Ser Val Ile Ile Ile Asn Asn Ser Thr Asn Val Val Ile Arg Ala Cys 
        115                 120                 125             


Asn Phe Glu Leu Cys Asp Asn Pro Phe Phe Ala Val Ser Lys Pro Met 
    130                 135                 140                 


Gly Thr Gln Thr His Thr Met Ile Phe Asp Asn Ala Phe Asn Cys Thr 
145                 150                 155                 160 


Phe Glu Tyr Ile Ser Asp Ala Phe Ser Leu Asp Val Ser Glu Lys Ser 
                165                 170                 175     


Gly Asn Phe Lys His Leu Arg Glu Phe Val Phe Lys Asn Lys Asp Gly 
            180                 185                 190         


Phe Leu Tyr Val Tyr Lys Gly Tyr Gln Pro Ile Asp Val Val Arg Asp 
        195                 200                 205             


Leu Pro Ser Gly Phe Asn Thr Leu Lys Pro Ile Phe Lys Leu Pro Leu 
    210                 215                 220                 


Gly Ile Asn Ile Thr Asn Phe Arg Ala Ile Leu Thr Ala Phe Ser Pro 
225                 230                 235                 240 


Ala Gln Asp Ile Trp Gly Thr Ser Ala Ala Ala Tyr Phe Val Gly Tyr 
                245                 250                 255     


Leu Lys Pro Thr Thr Phe Met Leu Lys Tyr Asp Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ser Gln Asn Pro Leu Ala Glu Leu Lys Cys 
        275                 280                 285             


Ser Val Lys Ser Phe Glu Ile Asp Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Val Pro Ser Gly Asp Val Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Pro Ser 
                325                 330                 335     


Val Tyr Ala Trp Glu Arg Lys Lys Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Thr Phe Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Met Gly Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr Ser 
            420                 425                 430         


Thr Gly Asn Tyr Asn Tyr Lys Tyr Arg Tyr Leu Arg His Gly Lys Leu 
        435                 440                 445             


Arg Pro Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp Gly 
    450                 455                 460                 


Lys Pro Cys Thr Pro Pro Ala Leu Asn Cys Tyr Trp Pro Leu Asn Asp 
465                 470                 475                 480 


Tyr Gly Phe Tyr Thr Thr Thr Gly Ile Gly Tyr Gln Pro Tyr Arg Val 
                485                 490                 495     


Val Val Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys Gly 
            500                 505                 510         


Pro Lys Leu Ser Thr Asp Leu Ile Lys Asn Gln Cys Val Asn Phe Asn 
        515                 520                 525             


Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Pro Ser Ser Lys Arg 
    530                 535                 540                 


Phe Gln Pro Phe Gln Gln Phe Gly Arg Asp Val Ser Asp Phe Thr Asp 
545                 550                 555                 560 


Ser Val Arg Asp Pro Lys Thr Ser Glu Ile Leu Asp Ile Ser Pro Cys 
                565                 570                 575     


Ala Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Ala Ser Ser 
            580                 585                 590         


Glu Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Asp Val Ser Thr 
        595                 600                 605             


Ala Ile His Ala Asp Gln Leu Thr Pro Ala Trp Arg Ile Tyr Ser Thr 
    610                 615                 620                 


Gly Asn Asn Val Phe Gln Thr Gln Ala Gly Cys Leu Ile Gly Ala Glu 
625                 630                 635                 640 


His Val Asp Thr Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile 
                645                 650                 655     


Cys Ala Ser Tyr His Thr Val Ser Leu Leu Arg Ser Thr Ser Gln Lys 
            660                 665                 670         


Ser Ile Val Ala Tyr Thr Met Ser Leu Gly Ala Asp Ser Ser Ile Ala 
        675                 680                 685             


Tyr Ser Asn Asn Thr Ile Ala Ile Pro Thr Asn Phe Ser Ile Ser Ile 
    690                 695                 700                 


Thr Thr Glu Val Met Pro Val Ser Met Ala Lys Thr Ser Val Asp Cys 
705                 710                 715                 720 


Asn Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ala Asn Leu Leu Leu 
                725                 730                 735     


Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Ser Gly Ile 
            740                 745                 750         


Ala Ala Glu Gln Asp Arg Asn Thr Arg Glu Val Phe Ala Gln Val Lys 
        755                 760                 765             


Gln Met Tyr Lys Thr Pro Thr Leu Lys Tyr Phe Gly Gly Phe Asn Phe 
    770                 775                 780                 


Ser Gln Ile Leu Pro Asp Pro Leu Lys Pro Thr Lys Arg Ser Phe Ile 
785                 790                 795                 800 


Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Met 
                805                 810                 815     


Lys Gln Tyr Gly Glu Cys Leu Gly Asp Ile Asn Ala Arg Asp Leu Ile 
            820                 825                 830         


Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr 
        835                 840                 845             


Asp Asp Met Ile Ala Ala Tyr Thr Ala Ala Leu Val Ser Gly Thr Ala 
    850                 855                 860                 


Thr Ala Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe 
865                 870                 875                 880 


Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn 
                885                 890                 895     


Val Leu Tyr Glu Asn Gln Lys Gln Ile Ala Asn Gln Phe Asn Lys Ala 
            900                 905                 910         


Ile Ser Gln Ile Gln Glu Ser Leu Thr Thr Thr Ser Thr Ala Leu Gly 
        915                 920                 925             


Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu 
    930                 935                 940                 


Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn 
945                 950                 955                 960 


Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp 
                965                 970                 975     


Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln 
            980                 985                 990         


Gln Leu Ile Arg Ala Ala Glu Ile  Arg Ala Ser Ala Asn  Leu Ala Ala 
        995                 1000                 1005             


Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp 
    1010                 1015                 1020             


Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser Phe Pro  Gln Ala Ala 
    1025                 1030                 1035             


Pro His  Gly Val Val Phe Leu  His Val Thr Tyr Val  Pro Ser Gln 
    1040                 1045                 1050             


Glu Arg  Asn Phe Thr Thr Ala  Pro Ala Ile Cys His  Glu Gly Lys 
    1055                 1060                 1065             


Ala Tyr  Phe Pro Arg Glu Gly  Val Phe Val Phe Asn  Gly Thr Ser 
    1070                 1075                 1080             


Trp Phe  Ile Thr Gln Arg Asn  Phe Phe Ser Pro Gln  Ile Ile Thr 
    1085                 1090                 1095             


Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys Asp Val  Val Ile Gly 
    1100                 1105                 1110             


Ile Ile  Asn Asn Thr Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp 
    1115                 1120                 1125             


Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser 
    1130                 1135                 1140             


Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val 
    1145                 1150                 1155             


Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys 
    1160                 1165                 1170             


Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr 
    1175                 1180                 1185             


Glu Gln  
    1190 


<210>  8
<211>  1351
<212>  PRT
<213>  human coronavirus HKU1

<400>  8

Met Phe Leu Ile Ile Phe Ile Leu Pro Thr Thr Leu Ala Val Ile Gly 
1               5                   10                  15      


Asp Phe Asn Cys Thr Asn Ser Phe Ile Asn Asp Tyr Asn Lys Thr Ile 
            20                  25                  30          


Pro Arg Ile Ser Glu Asp Val Val Asp Val Ser Leu Gly Leu Gly Thr 
        35                  40                  45              


Tyr Tyr Val Leu Asn Arg Val Tyr Leu Asn Thr Thr Leu Leu Phe Thr 
    50                  55                  60                  


Gly Tyr Phe Pro Lys Ser Gly Ala Asn Phe Arg Asp Leu Ala Leu Lys 
65                  70                  75                  80  


Gly Ser Ile Tyr Leu Ser Thr Leu Trp Tyr Lys Pro Pro Phe Leu Ser 
                85                  90                  95      


Asp Phe Asn Asn Gly Ile Phe Ser Lys Val Lys Asn Thr Lys Leu Tyr 
            100                 105                 110         


Val Asn Asn Thr Leu Tyr Ser Glu Phe Ser Thr Ile Val Ile Gly Ser 
        115                 120                 125             


Val Phe Val Asn Thr Ser Tyr Thr Ile Val Val Gln Pro His Asn Gly 
    130                 135                 140                 


Ile Leu Glu Ile Thr Ala Cys Gln Tyr Thr Met Cys Glu Tyr Pro His 
145                 150                 155                 160 


Thr Val Cys Lys Ser Lys Gly Ser Ile Arg Asn Glu Ser Trp His Ile 
                165                 170                 175     


Asp Ser Ser Glu Pro Leu Cys Leu Phe Lys Lys Asn Phe Thr Tyr Asn 
            180                 185                 190         


Val Ser Ala Asp Trp Leu Tyr Phe His Phe Tyr Gln Glu Arg Gly Val 
        195                 200                 205             


Phe Tyr Ala Tyr Tyr Ala Asp Val Gly Met Pro Thr Thr Phe Leu Phe 
    210                 215                 220                 


Ser Leu Tyr Leu Gly Thr Ile Leu Ser His Tyr Tyr Val Met Pro Leu 
225                 230                 235                 240 


Thr Cys Asn Ala Ile Ser Ser Asn Thr Asp Asn Glu Thr Leu Glu Tyr 
                245                 250                 255     


Trp Val Thr Pro Leu Ser Arg Arg Gln Tyr Leu Leu Asn Phe Asp Glu 
            260                 265                 270         


His Gly Val Ile Thr Asn Ala Val Asp Cys Ser Ser Ser Phe Leu Ser 
        275                 280                 285             


Glu Ile Gln Cys Lys Thr Gln Ser Phe Ala Pro Asn Thr Gly Val Tyr 
    290                 295                 300                 


Asp Leu Ser Gly Phe Thr Val Lys Pro Val Ala Thr Val Tyr Arg Arg 
305                 310                 315                 320 


Ile Pro Asn Leu Pro Asp Cys Asp Ile Asp Asn Trp Leu Asn Asn Val 
                325                 330                 335     


Ser Val Pro Ser Pro Leu Asn Trp Glu Arg Arg Ile Phe Ser Asn Cys 
            340                 345                 350         


Asn Phe Asn Leu Ser Thr Leu Leu Arg Leu Val His Val Asp Ser Phe 
        355                 360                 365             


Ser Cys Asn Asn Leu Asp Lys Ser Lys Ile Phe Gly Ser Cys Phe Asn 
    370                 375                 380                 


Ser Ile Thr Val Asp Lys Phe Ala Ile Pro Asn Arg Arg Arg Asp Asp 
385                 390                 395                 400 


Leu Gln Leu Gly Ser Ser Gly Phe Leu Gln Ser Ser Asn Tyr Lys Ile 
                405                 410                 415     


Asp Ile Ser Ser Ser Ser Cys Gln Leu Tyr Tyr Ser Leu Pro Leu Val 
            420                 425                 430         


Asn Val Thr Ile Asn Asn Phe Asn Pro Ser Ser Trp Asn Arg Arg Tyr 
        435                 440                 445             


Gly Phe Gly Ser Phe Asn Leu Ser Ser Tyr Asp Val Val Tyr Ser Asp 
    450                 455                 460                 


His Cys Phe Ser Val Asn Ser Asp Phe Cys Pro Cys Ala Asp Pro Ser 
465                 470                 475                 480 


Val Val Asn Ser Cys Ala Lys Ser Lys Pro Pro Ser Ala Ile Cys Pro 
                485                 490                 495     


Ala Gly Thr Lys Tyr Arg His Cys Asp Leu Asp Thr Thr Leu Tyr Val 
            500                 505                 510         


Lys Asn Trp Cys Arg Cys Ser Cys Leu Pro Asp Pro Ile Ser Thr Tyr 
        515                 520                 525             


Ser Pro Asn Thr Cys Pro Gln Lys Lys Val Val Val Gly Ile Gly Glu 
    530                 535                 540                 


His Cys Pro Gly Leu Gly Ile Asn Glu Glu Lys Cys Gly Thr Gln Leu 
545                 550                 555                 560 


Asn His Ser Ser Cys Phe Cys Ser Pro Asp Ala Phe Leu Gly Trp Ser 
                565                 570                 575     


Phe Asp Ser Cys Ile Ser Asn Asn Arg Cys Asn Ile Phe Ser Asn Phe 
            580                 585                 590         


Ile Phe Asn Gly Ile Asn Ser Gly Thr Thr Cys Ser Asn Asp Leu Leu 
        595                 600                 605             


Tyr Ser Asn Thr Glu Ile Ser Thr Gly Val Cys Val Asn Tyr Asp Leu 
    610                 615                 620                 


Tyr Gly Ile Thr Gly Gln Gly Ile Phe Lys Glu Val Ser Ala Ala Tyr 
625                 630                 635                 640 


Tyr Asn Asn Trp Gln Asn Leu Leu Tyr Asp Ser Asn Gly Asn Ile Ile 
                645                 650                 655     


Gly Phe Lys Asp Phe Leu Thr Asn Lys Thr Tyr Thr Ile Leu Pro Cys 
            660                 665                 670         


Tyr Ser Gly Arg Val Ser Ala Ala Phe Tyr Gln Asn Ser Ser Ser Pro 
        675                 680                 685             


Ala Leu Leu Tyr Arg Asn Leu Lys Cys Ser Tyr Val Leu Asn Asn Ile 
    690                 695                 700                 


Ser Phe Ile Ser Gln Pro Phe Tyr Phe Asp Ser Tyr Leu Gly Cys Val 
705                 710                 715                 720 


Leu Asn Ala Val Asn Leu Thr Ser Tyr Ser Val Ser Ser Cys Asp Leu 
                725                 730                 735     


Arg Met Gly Ser Gly Phe Cys Ile Asp Tyr Ala Leu Pro Ser Ser Arg 
            740                 745                 750         


Arg Lys Arg Arg Gly Ile Ser Ser Pro Tyr Arg Phe Val Thr Phe Glu 
        755                 760                 765             


Pro Phe Asn Val Ser Phe Val Asn Asp Ser Val Glu Thr Val Gly Gly 
    770                 775                 780                 


Leu Phe Glu Ile Gln Ile Pro Thr Asn Phe Thr Ile Ala Gly His Glu 
785                 790                 795                 800 


Glu Phe Ile Gln Thr Ser Ser Pro Lys Val Thr Ile Asp Cys Ser Ala 
                805                 810                 815     


Phe Val Cys Ser Asn Tyr Ala Ala Cys His Asp Leu Leu Ser Glu Tyr 
            820                 825                 830         


Gly Thr Phe Cys Asp Asn Ile Asn Ser Ile Leu Asn Glu Val Asn Asp 
        835                 840                 845             


Leu Leu Asp Ile Thr Gln Leu Gln Val Ala Asn Ala Leu Met Gln Gly 
    850                 855                 860                 


Val Thr Leu Ser Ser Asn Leu Asn Thr Asn Leu His Ser Asp Val Asp 
865                 870                 875                 880 


Asn Ile Asp Phe Lys Ser Leu Leu Gly Cys Leu Gly Ser Gln Cys Gly 
                885                 890                 895     


Ser Ser Ser Arg Ser Leu Leu Glu Asp Leu Leu Phe Asn Lys Val Lys 
            900                 905                 910         


Leu Ser Asp Val Gly Phe Val Glu Ala Tyr Asn Asn Cys Thr Gly Gly 
        915                 920                 925             


Ser Glu Ile Arg Asp Leu Leu Cys Val Gln Ser Phe Asn Gly Ile Lys 
    930                 935                 940                 


Val Leu Pro Pro Ile Leu Ser Glu Thr Gln Ile Ser Gly Tyr Thr Thr 
945                 950                 955                 960 


Ala Ala Thr Val Ala Ala Met Phe Pro Pro Trp Ser Ala Ala Ala Gly 
                965                 970                 975     


Val Pro Phe Ser Leu Asn Val Gln Tyr Arg Ile Asn Gly Leu Gly Val 
            980                 985                 990         


Thr Met Asp Val Leu Asn Lys Asn  Gln Lys Leu Ile Ala  Asn Ala Phe 
        995                 1000                 1005             


Asn Lys  Ala Leu Leu Ser Ile  Gln Asn Gly Phe Thr  Ala Thr Asn 
    1010                 1015                 1020             


Ser Ala  Leu Ala Lys Ile Gln  Ser Val Val Asn Ala  Asn Ala Gln 
    1025                 1030                 1035             


Ala Leu  Asn Ser Leu Leu Gln  Gln Leu Phe Asn Lys  Phe Gly Ala 
    1040                 1045                 1050             


Ile Ser  Ser Ser Leu Gln Glu  Ile Leu Ser Arg Leu  Asp Asn Leu 
    1055                 1060                 1065             


Glu Ala  Gln Val Gln Ile Asp  Arg Leu Ile Asn Gly  Arg Leu Thr 
    1070                 1075                 1080             


Ala Leu  Asn Ala Tyr Val Ser  Gln Gln Leu Ser Asp  Ile Thr Leu 
    1085                 1090                 1095             


Ile Lys  Ala Gly Ala Ser Arg  Ala Ile Glu Lys Val  Asn Glu Cys 
    1100                 1105                 1110             


Val Lys  Ser Gln Ser Pro Arg  Ile Asn Phe Cys Gly  Asn Gly Asn 
    1115                 1120                 1125             


His Ile  Leu Ser Leu Val Gln  Asn Ala Pro Tyr Gly  Leu Leu Phe 
    1130                 1135                 1140             


Ile His  Phe Ser Tyr Lys Pro  Thr Ser Phe Lys Thr  Val Leu Val 
    1145                 1150                 1155             


Ser Pro  Gly Leu Cys Leu Ser  Gly Asp Arg Gly Ile  Ala Pro Lys 
    1160                 1165                 1170             


Gln Gly  Tyr Phe Ile Lys Gln  Asn Asp Ser Trp Met  Phe Thr Gly 
    1175                 1180                 1185             


Ser Ser  Tyr Tyr Tyr Pro Glu  Pro Ile Ser Asp Lys  Asn Val Val 
    1190                 1195                 1200             


Phe Met  Asn Ser Cys Ser Val  Asn Phe Thr Lys Ala  Pro Phe Ile 
    1205                 1210                 1215             


Tyr Leu  Asn Asn Ser Ile Pro  Asn Leu Ser Asp Phe  Glu Ala Glu 
    1220                 1225                 1230             


Leu Ser  Leu Trp Phe Lys Asn  His Thr Ser Ile Ala  Pro Asn Leu 
    1235                 1240                 1245             


Thr Phe  Asn Ser His Ile Asn  Ala Thr Phe Leu Asp  Leu Tyr Tyr 
    1250                 1255                 1260             


Glu Met  Asn Val Ile Gln Glu  Ser Ile Lys Ser Leu  Asn Ser Ser 
    1265                 1270                 1275             


Phe Ile  Asn Leu Lys Glu Ile  Gly Thr Tyr Glu Met  Tyr Val Lys 
    1280                 1285                 1290             


Trp Pro  Trp Tyr Ile Trp Leu  Leu Ile Val Ile Leu  Phe Ile Ile 
    1295                 1300                 1305             


Phe Leu  Met Ile Leu Phe Phe  Ile Cys Cys Cys Thr  Gly Cys Gly 
    1310                 1315                 1320             


Ser Ala  Cys Phe Ser Lys Cys  His Asn Cys Cys Asp  Glu Tyr Gly 
    1325                 1330                 1335             


Gly His  Asn Asp Phe Val Ile  Lys Ala Ser His Asp  Asp 
    1340                 1345                 1350     


<210>  9
<211>  1276
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  9

Met Phe Leu Ile Ile Phe Ile Leu Pro Thr Thr Leu Ala Val Ile Gly 
1               5                   10                  15      


Asp Phe Asn Cys Thr Asn Ser Phe Ile Asn Asp Tyr Asn Lys Thr Ile 
            20                  25                  30          


Pro Arg Ile Ser Glu Asp Val Val Asp Val Ser Leu Gly Leu Gly Thr 
        35                  40                  45              


Tyr Tyr Val Leu Asn Arg Val Tyr Leu Asn Thr Thr Leu Leu Phe Thr 
    50                  55                  60                  


Gly Tyr Phe Pro Lys Ser Gly Ala Asn Phe Arg Asp Leu Ala Leu Lys 
65                  70                  75                  80  


Gly Ser Ile Tyr Leu Ser Thr Leu Trp Tyr Lys Pro Pro Phe Leu Ser 
                85                  90                  95      


Asp Phe Asn Asn Gly Ile Phe Ser Lys Val Lys Asn Thr Lys Leu Tyr 
            100                 105                 110         


Val Asn Asn Thr Leu Tyr Ser Glu Phe Ser Thr Ile Val Ile Gly Ser 
        115                 120                 125             


Val Phe Val Asn Thr Ser Tyr Thr Ile Val Val Gln Pro His Asn Gly 
    130                 135                 140                 


Ile Leu Glu Ile Thr Ala Cys Gln Tyr Thr Met Cys Glu Tyr Pro His 
145                 150                 155                 160 


Thr Val Cys Lys Ser Lys Gly Ser Ile Arg Asn Glu Ser Trp His Ile 
                165                 170                 175     


Asp Ser Ser Glu Pro Leu Cys Leu Phe Lys Lys Asn Phe Thr Tyr Asn 
            180                 185                 190         


Val Ser Ala Asp Trp Leu Tyr Phe His Phe Tyr Gln Glu Arg Gly Val 
        195                 200                 205             


Phe Tyr Ala Tyr Tyr Ala Asp Val Gly Met Pro Thr Thr Phe Leu Phe 
    210                 215                 220                 


Ser Leu Tyr Leu Gly Thr Ile Leu Ser His Tyr Tyr Val Met Pro Leu 
225                 230                 235                 240 


Thr Cys Asn Ala Ile Ser Ser Asn Thr Asp Asn Glu Thr Leu Glu Tyr 
                245                 250                 255     


Trp Val Thr Pro Leu Ser Arg Arg Gln Tyr Leu Leu Asn Phe Asp Glu 
            260                 265                 270         


His Gly Val Ile Thr Asn Ala Val Asp Cys Ser Ser Ser Phe Leu Ser 
        275                 280                 285             


Glu Ile Gln Cys Lys Thr Gln Ser Phe Ala Pro Asn Thr Gly Val Tyr 
    290                 295                 300                 


Asp Leu Ser Gly Phe Thr Val Lys Pro Val Ala Thr Val Tyr Arg Arg 
305                 310                 315                 320 


Ile Pro Asn Leu Pro Asp Cys Asp Ile Asp Asn Trp Leu Asn Asn Val 
                325                 330                 335     


Ser Val Pro Ser Pro Leu Asn Trp Glu Arg Arg Ile Phe Ser Asn Cys 
            340                 345                 350         


Asn Phe Asn Leu Ser Thr Leu Leu Arg Leu Val His Val Asp Ser Phe 
        355                 360                 365             


Ser Cys Asn Asn Leu Asp Lys Ser Lys Ile Phe Gly Ser Cys Phe Asn 
    370                 375                 380                 


Ser Ile Thr Val Asp Lys Phe Ala Ile Pro Asn Arg Arg Arg Asp Asp 
385                 390                 395                 400 


Leu Gln Leu Gly Ser Ser Gly Phe Leu Gln Ser Ser Asn Tyr Lys Ile 
                405                 410                 415     


Asp Ile Ser Ser Ser Ser Cys Gln Leu Tyr Tyr Ser Leu Pro Leu Val 
            420                 425                 430         


Asn Val Thr Ile Asn Asn Phe Asn Pro Ser Ser Trp Asn Arg Arg Tyr 
        435                 440                 445             


Gly Phe Gly Ser Phe Asn Leu Ser Ser Tyr Asp Val Val Tyr Ser Asp 
    450                 455                 460                 


His Cys Phe Ser Val Asn Ser Asp Phe Cys Pro Cys Ala Asp Pro Ser 
465                 470                 475                 480 


Val Val Asn Ser Cys Ala Lys Ser Lys Pro Pro Ser Ala Ile Cys Pro 
                485                 490                 495     


Ala Gly Thr Lys Tyr Arg His Cys Asp Leu Asp Thr Thr Leu Tyr Val 
            500                 505                 510         


Lys Asn Trp Cys Arg Cys Ser Cys Leu Pro Asp Pro Ile Ser Thr Tyr 
        515                 520                 525             


Ser Pro Asn Thr Cys Pro Gln Lys Lys Val Val Val Gly Ile Gly Glu 
    530                 535                 540                 


His Cys Pro Gly Leu Gly Ile Asn Glu Glu Lys Cys Gly Thr Gln Leu 
545                 550                 555                 560 


Asn His Ser Ser Cys Phe Cys Ser Pro Asp Ala Phe Leu Gly Trp Ser 
                565                 570                 575     


Phe Asp Ser Cys Ile Ser Asn Asn Arg Cys Asn Ile Phe Ser Asn Phe 
            580                 585                 590         


Ile Phe Asn Gly Ile Asn Ser Gly Thr Thr Cys Ser Asn Asp Leu Leu 
        595                 600                 605             


Tyr Ser Asn Thr Glu Ile Ser Thr Gly Val Cys Val Asn Tyr Asp Leu 
    610                 615                 620                 


Tyr Gly Ile Thr Gly Gln Gly Ile Phe Lys Glu Val Ser Ala Ala Tyr 
625                 630                 635                 640 


Tyr Asn Asn Trp Gln Asn Leu Leu Tyr Asp Ser Asn Gly Asn Ile Ile 
                645                 650                 655     


Gly Phe Lys Asp Phe Leu Thr Asn Lys Thr Tyr Thr Ile Leu Pro Cys 
            660                 665                 670         


Tyr Ser Gly Arg Val Ser Ala Ala Phe Tyr Gln Asn Ser Ser Ser Pro 
        675                 680                 685             


Ala Leu Leu Tyr Arg Asn Leu Lys Cys Ser Tyr Val Leu Asn Asn Ile 
    690                 695                 700                 


Ser Phe Ile Ser Gln Pro Phe Tyr Phe Asp Ser Tyr Leu Gly Cys Val 
705                 710                 715                 720 


Leu Asn Ala Val Asn Leu Thr Ser Tyr Ser Val Ser Ser Cys Asp Leu 
                725                 730                 735     


Arg Met Gly Ser Gly Phe Cys Ile Asp Tyr Ala Leu Pro Ser Ser Gly 
            740                 745                 750         


Gly Ser Gly Ser Gly Ile Ser Ser Pro Tyr Arg Phe Val Thr Phe Glu 
        755                 760                 765             


Pro Phe Asn Val Ser Phe Val Asn Asp Ser Val Glu Thr Val Gly Gly 
    770                 775                 780                 


Leu Phe Glu Ile Gln Ile Pro Thr Asn Phe Thr Ile Ala Gly His Glu 
785                 790                 795                 800 


Glu Phe Ile Gln Thr Ser Ser Pro Lys Val Thr Ile Asp Cys Ser Ala 
                805                 810                 815     


Phe Val Cys Ser Asn Tyr Ala Ala Cys His Asp Leu Leu Ser Glu Tyr 
            820                 825                 830         


Gly Thr Phe Cys Asp Asn Ile Asn Ser Ile Leu Asn Glu Val Asn Asp 
        835                 840                 845             


Leu Leu Asp Ile Thr Gln Leu Gln Val Ala Asn Ala Leu Met Gln Gly 
    850                 855                 860                 


Val Thr Leu Ser Ser Asn Leu Asn Thr Asn Leu His Ser Asp Val Asp 
865                 870                 875                 880 


Asn Ile Asp Phe Lys Ser Leu Leu Gly Cys Leu Gly Ser Gln Cys Gly 
                885                 890                 895     


Ser Ser Ser Arg Ser Leu Leu Glu Asp Leu Leu Phe Asn Lys Val Lys 
            900                 905                 910         


Leu Ser Asp Val Gly Phe Val Glu Ala Tyr Asn Asn Cys Thr Gly Gly 
        915                 920                 925             


Ser Glu Ile Arg Asp Leu Leu Cys Val Gln Ser Phe Asn Gly Ile Lys 
    930                 935                 940                 


Val Leu Pro Pro Ile Leu Ser Glu Thr Gln Ile Ser Gly Tyr Thr Thr 
945                 950                 955                 960 


Ala Ala Thr Val Ala Ala Met Phe Pro Pro Trp Ser Ala Ala Ala Gly 
                965                 970                 975     


Val Pro Phe Ser Leu Asn Val Gln Tyr Arg Ile Asn Gly Leu Gly Val 
            980                 985                 990         


Thr Met Asp Val Leu Asn Lys Asn  Gln Lys Leu Ile Ala  Asn Ala Phe 
        995                 1000                 1005             


Asn Lys  Ala Leu Leu Ser Ile  Gln Asn Gly Phe Thr  Ala Thr Asn 
    1010                 1015                 1020             


Ser Ala  Leu Ala Lys Ile Gln  Ser Val Val Asn Ala  Asn Ala Gln 
    1025                 1030                 1035             


Ala Leu  Asn Ser Leu Leu Gln  Gln Leu Phe Asn Lys  Phe Gly Ala 
    1040                 1045                 1050             


Ile Ser  Ser Ser Leu Gln Glu  Ile Leu Ser Arg Leu  Asp Pro Pro 
    1055                 1060                 1065             


Glu Ala  Gln Val Gln Ile Asp  Arg Leu Ile Asn Gly  Arg Leu Thr 
    1070                 1075                 1080             


Ala Leu  Asn Ala Tyr Val Ser  Gln Gln Leu Ser Asp  Ile Thr Leu 
    1085                 1090                 1095             


Ile Lys  Ala Gly Ala Ser Arg  Ala Ile Glu Lys Val  Asn Glu Cys 
    1100                 1105                 1110             


Val Lys  Ser Gln Ser Pro Arg  Ile Asn Phe Cys Gly  Asn Gly Asn 
    1115                 1120                 1125             


His Ile  Leu Ser Leu Val Gln  Asn Ala Pro Tyr Gly  Leu Leu Phe 
    1130                 1135                 1140             


Ile His  Phe Ser Tyr Lys Pro  Thr Ser Phe Lys Thr  Val Leu Val 
    1145                 1150                 1155             


Ser Pro  Gly Leu Cys Leu Ser  Gly Asp Arg Gly Ile  Ala Pro Lys 
    1160                 1165                 1170             


Gln Gly  Tyr Phe Ile Lys Gln  Asn Asp Ser Trp Met  Phe Thr Gly 
    1175                 1180                 1185             


Ser Ser  Tyr Tyr Tyr Pro Glu  Pro Ile Ser Asp Lys  Asn Val Val 
    1190                 1195                 1200             


Phe Met  Asn Ser Cys Ser Val  Asn Phe Thr Lys Ala  Pro Phe Ile 
    1205                 1210                 1215             


Tyr Leu  Asn Asn Ser Ile Pro  Asn Leu Ser Asp Phe  Glu Ala Glu 
    1220                 1225                 1230             


Leu Ser  Leu Trp Phe Lys Asn  His Thr Ser Ile Ala  Pro Asn Leu 
    1235                 1240                 1245             


Thr Phe  Asn Ser His Ile Asn  Ala Thr Phe Leu Asp  Leu Tyr Tyr 
    1250                 1255                 1260             


Glu Met  Asn Val Ile Gln Glu  Ser Ile Lys Ser Leu  Asn 
    1265                 1270                 1275     


<210>  10
<211>  1362
<212>  PRT
<213>  human coronavirus OC43

<400>  10

Met Phe Leu Ile Leu Leu Ile Ser Leu Pro Thr Ala Phe Ala Val Ile 
1               5                   10                  15      


Gly Asp Leu Lys Cys Pro Leu Asp Ser Arg Thr Gly Ser Leu Asn Asn 
            20                  25                  30          


Ile Asp Thr Gly Pro Pro Ser Ile Ser Thr Ala Thr Val Asp Val Thr 
        35                  40                  45              


Asn Gly Leu Gly Thr Tyr Tyr Val Leu Asp Arg Val Tyr Leu Asn Thr 
    50                  55                  60                  


Thr Leu Phe Leu Asn Gly Tyr Tyr Pro Thr Ser Gly Ser Thr Tyr Arg 
65                  70                  75                  80  


Asn Met Ala Leu Lys Gly Thr Asp Lys Leu Ser Thr Leu Trp Phe Lys 
                85                  90                  95      


Pro Pro Phe Leu Ser Asp Phe Ile Asn Gly Ile Phe Ala Lys Val Lys 
            100                 105                 110         


Asn Thr Lys Val Phe Lys Asp Gly Val Met Tyr Ser Glu Phe Pro Ala 
        115                 120                 125             


Ile Thr Ile Gly Ser Thr Phe Val Asn Thr Ser Tyr Ser Val Val Val 
    130                 135                 140                 


Gln Pro Arg Thr Ile Asn Ser Thr Gln Asp Gly Val Asn Lys Leu Gln 
145                 150                 155                 160 


Gly Leu Leu Glu Val Ser Val Cys Gln Tyr Asn Met Cys Glu Tyr Pro 
                165                 170                 175     


His Thr Ile Cys His Pro Lys Leu Gly Asn His Phe Lys Glu Leu Trp 
            180                 185                 190         


His Met Asp Thr Gly Val Val Ser Cys Leu Tyr Lys Arg Asn Phe Thr 
        195                 200                 205             


Tyr Asp Val Asn Ala Thr Tyr Leu Tyr Phe His Phe Tyr Gln Glu Gly 
    210                 215                 220                 


Gly Thr Phe Tyr Ala Tyr Phe Thr Asp Thr Gly Val Val Thr Lys Phe 
225                 230                 235                 240 


Leu Phe Asn Val Tyr Leu Gly Met Ala Leu Ser His Tyr Tyr Val Met 
                245                 250                 255     


Pro Leu Thr Cys Ile Ser Arg Arg Asp Ile Gly Phe Thr Leu Glu Tyr 
            260                 265                 270         


Trp Val Thr Pro Leu Thr Ser Arg Gln Tyr Leu Leu Ala Phe Asn Gln 
        275                 280                 285             


Asp Gly Ile Ile Phe Asn Ala Val Asp Cys Met Ser Asp Phe Met Ser 
    290                 295                 300                 


Glu Ile Lys Cys Lys Thr Gln Ser Ile Ala Pro Pro Thr Gly Val Tyr 
305                 310                 315                 320 


Glu Leu Asn Gly Tyr Thr Val Gln Pro Ile Ala Asp Val Tyr Arg Arg 
                325                 330                 335     


Lys Pro Asp Leu Pro Asn Cys Asn Ile Glu Ala Trp Leu Asn Asp Lys 
            340                 345                 350         


Ser Val Pro Ser Pro Leu Asn Trp Glu Arg Lys Thr Phe Ser Asn Cys 
        355                 360                 365             


Asn Phe Asn Met Ser Ser Leu Met Ser Phe Ile Gln Ala Asp Ser Phe 
    370                 375                 380                 


Thr Cys Asn Asn Ile Asp Ala Ala Lys Ile Tyr Gly Met Cys Phe Ser 
385                 390                 395                 400 


Ser Ile Thr Ile Asp Lys Phe Ala Ile Pro Asn Gly Arg Lys Val Asp 
                405                 410                 415     


Leu Gln Leu Gly Asn Leu Gly Tyr Leu Gln Ser Phe Asn Tyr Arg Ile 
            420                 425                 430         


Asp Thr Thr Ala Thr Ser Cys Gln Leu Tyr Tyr Asn Leu Pro Ala Ala 
        435                 440                 445             


Asn Val Ser Val Ser Arg Phe Asn Pro Ser Thr Trp Asn Lys Arg Phe 
    450                 455                 460                 


Gly Phe Ile Glu Asn Ser Val Phe Lys Pro Gln Pro Ala Gly Val Leu 
465                 470                 475                 480 


Thr Asn His Asp Val Val Tyr Ala Gln His Cys Phe Lys Ala Pro Lys 
                485                 490                 495     


Asn Phe Cys Pro Cys Lys Leu Asn Ser Ser Leu Cys Val Gly Ser Gly 
            500                 505                 510         


Pro Gly Lys Asn Asn Gly Ile Gly Thr Cys Pro Ala Gly Thr Asn Tyr 
        515                 520                 525             


Leu Thr Cys His Asn Leu Cys Asn Pro Asp Pro Ile Thr Phe Thr Gly 
    530                 535                 540                 


Pro Tyr Lys Cys Pro Gln Thr Lys Ser Leu Val Gly Ile Gly Glu His 
545                 550                 555                 560 


Cys Ser Gly Leu Ala Val Lys Ser Asp Tyr Cys Gly Gly Asn Pro Cys 
                565                 570                 575     


Thr Cys Gln Pro Gln Ala Phe Leu Gly Trp Ser Ala Asp Ser Cys Leu 
            580                 585                 590         


Gln Gly Asp Lys Cys Asn Ile Phe Ala Asn Leu Ile Leu His Asp Val 
        595                 600                 605             


Asn Ser Gly Leu Thr Cys Ser Thr Asp Leu Gln Lys Ala Asn Thr Asp 
    610                 615                 620                 


Ile Lys Leu Gly Val Cys Val Asn Tyr Asp Leu Tyr Gly Ile Ser Gly 
625                 630                 635                 640 


Gln Gly Ile Phe Val Glu Val Asn Ala Thr Tyr Tyr Asn Ser Trp Gln 
                645                 650                 655     


Asn Leu Leu Tyr Asp Ser Asn Gly Asn Leu Tyr Gly Phe Arg Asp Tyr 
            660                 665                 670         


Ile Thr Asn Arg Thr Phe Met Ile Arg Ser Cys Tyr Ser Gly Arg Val 
        675                 680                 685             


Ser Ala Ala Phe His Ala Asn Ser Ser Glu Pro Ala Leu Leu Phe Arg 
    690                 695                 700                 


Asn Ile Lys Cys Asn Tyr Val Phe Asn Asn Ser Leu Ile Arg Gln Leu 
705                 710                 715                 720 


Gln Pro Ile Asn Tyr Phe Asp Ser Tyr Leu Gly Cys Val Val Asn Ala 
                725                 730                 735     


Tyr Asn Ser Thr Ala Ile Ser Val Gln Thr Cys Asp Leu Thr Val Gly 
            740                 745                 750         


Ser Gly Tyr Cys Val Asp Tyr Ser Lys Asn Arg Arg Ser Arg Arg Ala 
        755                 760                 765             


Ile Thr Thr Gly Tyr Arg Phe Thr Asn Phe Glu Pro Phe Thr Val Asn 
    770                 775                 780                 


Ser Val Asn Asp Ser Leu Glu Pro Val Gly Gly Leu Tyr Glu Ile Gln 
785                 790                 795                 800 


Ile Pro Ser Glu Phe Thr Ile Gly Asn Met Glu Glu Phe Ile Gln Thr 
                805                 810                 815     


Ser Ser Pro Lys Val Thr Ile Asp Cys Ala Ala Phe Val Cys Gly Asp 
            820                 825                 830         


Tyr Ala Ala Cys Lys Ser Gln Leu Val Glu Tyr Gly Ser Phe Cys Asp 
        835                 840                 845             


Asn Ile Asn Ala Ile Leu Thr Glu Val Asn Glu Leu Leu Asp Thr Thr 
    850                 855                 860                 


Gln Leu Gln Val Ala Asn Ser Leu Met Asn Gly Val Thr Leu Ser Thr 
865                 870                 875                 880 


Lys Leu Lys Asp Gly Val Asn Phe Asn Val Asp Asp Ile Asn Phe Ser 
                885                 890                 895     


Ser Val Leu Gly Cys Leu Gly Ser Glu Cys Ser Lys Ala Ser Ser Arg 
            900                 905                 910         


Ser Ala Ile Glu Asp Leu Leu Phe Asp Lys Val Lys Leu Ser Asp Val 
        915                 920                 925             


Gly Phe Val Ala Ala Tyr Asn Asn Cys Thr Gly Gly Ala Glu Ile Arg 
    930                 935                 940                 


Asp Leu Ile Cys Val Gln Ser Tyr Lys Gly Ile Lys Val Leu Pro Pro 
945                 950                 955                 960 


Leu Leu Ser Glu Asn Gln Ile Ser Gly Tyr Thr Leu Ala Ala Thr Ser 
                965                 970                 975     


Ala Ser Leu Phe Pro Pro Trp Thr Ala Ala Ala Gly Val Pro Phe Tyr 
            980                 985                 990         


Leu Asn Val Gln Tyr Arg Ile Asn  Gly Leu Gly Val Thr  Met Asp Val 
        995                 1000                 1005             


Leu Ser  Gln Asn Gln Lys Leu  Ile Ala Asn Ala Phe  Asn Asn Ala 
    1010                 1015                 1020             


Leu Asp  Ala Ile Gln Glu Gly  Phe Asp Ala Thr Asn  Ser Ala Leu 
    1025                 1030                 1035             


Val Lys  Ile Gln Ala Val Val  Asn Ala Asn Ala Glu  Ala Leu Asn 
    1040                 1045                 1050             


Asn Leu  Leu Gln Gln Leu Ser  Asn Arg Phe Gly Ala  Ile Ser Ser 
    1055                 1060                 1065             


Ser Leu  Gln Glu Ile Leu Ser  Arg Leu Asp Ala Leu  Glu Ala Glu 
    1070                 1075                 1080             


Ala Gln  Ile Asp Arg Leu Ile  Asn Gly Arg Leu Thr  Ala Leu Asn 
    1085                 1090                 1095             


Ala Tyr  Val Ser Gln Gln Leu  Ser Asp Ser Thr Leu  Val Lys Phe 
    1100                 1105                 1110             


Ser Ala  Ala Gln Ala Met Glu  Lys Val Asn Glu Cys  Val Lys Ser 
    1115                 1120                 1125             


Gln Ser  Ser Arg Ile Asn Phe  Cys Gly Asn Gly Asn  His Ile Ile 
    1130                 1135                 1140             


Ser Leu  Val Gln Asn Ala Pro  Tyr Gly Leu Tyr Phe  Ile His Phe 
    1145                 1150                 1155             


Ser Tyr  Val Pro Thr Lys Tyr  Val Thr Ala Lys Val  Ser Pro Gly 
    1160                 1165                 1170             


Leu Cys  Ile Ala Gly Asp Arg  Gly Ile Ala Pro Lys  Ser Gly Tyr 
    1175                 1180                 1185             


Phe Val  Asn Val Asn Asn Thr  Trp Met Tyr Thr Gly  Ser Gly Tyr 
    1190                 1195                 1200             


Tyr Tyr  Pro Glu Pro Ile Thr  Glu Asn Asn Val Val  Val Met Ser 
    1205                 1210                 1215             


Thr Cys  Ala Val Asn Tyr Thr  Lys Ala Pro Tyr Val  Met Leu Asn 
    1220                 1225                 1230             


Thr Ser  Thr Pro Asn Leu Pro  Asp Phe Arg Glu Glu  Leu Asp Gln 
    1235                 1240                 1245             


Trp Phe  Lys Asn Gln Thr Ser  Val Ala Pro Asp Leu  Ser Leu Asp 
    1250                 1255                 1260             


Tyr Ile  Asn Val Thr Phe Leu  Asp Leu Gln Val Glu  Met Asn Arg 
    1265                 1270                 1275             


Leu Gln  Glu Ala Ile Lys Val  Leu Asn Gln Ser Tyr  Ile Asn Leu 
    1280                 1285                 1290             


Lys Asp  Ile Gly Thr Tyr Glu  Tyr Tyr Val Lys Trp  Pro Trp Tyr 
    1295                 1300                 1305             


Val Trp  Leu Leu Ile Gly Leu  Ala Gly Val Ala Met  Leu Val Leu 
    1310                 1315                 1320             


Leu Phe  Phe Ile Cys Cys Cys  Thr Gly Cys Gly Thr  Ser Cys Phe 
    1325                 1330                 1335             


Lys Lys  Cys Gly Gly Cys Cys  Asp Asp Tyr Thr Gly  Tyr Gln Glu 
    1340                 1345                 1350             


Leu Val  Ile Lys Thr Ser His  Asp Asp 
    1355                 1360         


<210>  11
<211>  1287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  11

Met Phe Leu Ile Leu Leu Ile Ser Leu Pro Thr Ala Phe Ala Val Ile 
1               5                   10                  15      


Gly Asp Leu Lys Cys Pro Leu Asp Ser Arg Thr Gly Ser Leu Asn Asn 
            20                  25                  30          


Ile Asp Thr Gly Pro Pro Ser Ile Ser Thr Ala Thr Val Asp Val Thr 
        35                  40                  45              


Asn Gly Leu Gly Thr Tyr Tyr Val Leu Asp Arg Val Tyr Leu Asn Thr 
    50                  55                  60                  


Thr Leu Phe Leu Asn Gly Tyr Tyr Pro Thr Ser Gly Ser Thr Tyr Arg 
65                  70                  75                  80  


Asn Met Ala Leu Lys Gly Thr Asp Lys Leu Ser Thr Leu Trp Phe Lys 
                85                  90                  95      


Pro Pro Phe Leu Ser Asp Phe Ile Asn Gly Ile Phe Ala Lys Val Lys 
            100                 105                 110         


Asn Thr Lys Val Phe Lys Asp Gly Val Met Tyr Ser Glu Phe Pro Ala 
        115                 120                 125             


Ile Thr Ile Gly Ser Thr Phe Val Asn Thr Ser Tyr Ser Val Val Val 
    130                 135                 140                 


Gln Pro Arg Thr Ile Asn Ser Thr Gln Asp Gly Val Asn Lys Leu Gln 
145                 150                 155                 160 


Gly Leu Leu Glu Val Ser Val Cys Gln Tyr Asn Met Cys Glu Tyr Pro 
                165                 170                 175     


His Thr Ile Cys His Pro Lys Leu Gly Asn His Phe Lys Glu Leu Trp 
            180                 185                 190         


His Met Asp Thr Gly Val Val Ser Cys Leu Tyr Lys Arg Asn Phe Thr 
        195                 200                 205             


Tyr Asp Val Asn Ala Thr Tyr Leu Tyr Phe His Phe Tyr Gln Glu Gly 
    210                 215                 220                 


Gly Thr Phe Tyr Ala Tyr Phe Thr Asp Thr Gly Val Val Thr Lys Phe 
225                 230                 235                 240 


Leu Phe Asn Val Tyr Leu Gly Met Ala Leu Ser His Tyr Tyr Val Met 
                245                 250                 255     


Pro Leu Thr Cys Ile Ser Arg Arg Asp Ile Gly Phe Thr Leu Glu Tyr 
            260                 265                 270         


Trp Val Thr Pro Leu Thr Ser Arg Gln Tyr Leu Leu Ala Phe Asn Gln 
        275                 280                 285             


Asp Gly Ile Ile Phe Asn Ala Val Asp Cys Met Ser Asp Phe Met Ser 
    290                 295                 300                 


Glu Ile Lys Cys Lys Thr Gln Ser Ile Ala Pro Pro Thr Gly Val Tyr 
305                 310                 315                 320 


Glu Leu Asn Gly Tyr Thr Val Gln Pro Ile Ala Asp Val Tyr Arg Arg 
                325                 330                 335     


Lys Pro Asp Leu Pro Asn Cys Asn Ile Glu Ala Trp Leu Asn Asp Lys 
            340                 345                 350         


Ser Val Pro Ser Pro Leu Asn Trp Glu Arg Lys Thr Phe Ser Asn Cys 
        355                 360                 365             


Asn Phe Asn Met Ser Ser Leu Met Ser Phe Ile Gln Ala Asp Ser Phe 
    370                 375                 380                 


Thr Cys Asn Asn Ile Asp Ala Ala Lys Ile Tyr Gly Met Cys Phe Ser 
385                 390                 395                 400 


Ser Ile Thr Ile Asp Lys Phe Ala Ile Pro Asn Gly Arg Lys Val Asp 
                405                 410                 415     


Leu Gln Leu Gly Asn Leu Gly Tyr Leu Gln Ser Phe Asn Tyr Arg Ile 
            420                 425                 430         


Asp Thr Thr Ala Thr Ser Cys Gln Leu Tyr Tyr Asn Leu Pro Ala Ala 
        435                 440                 445             


Asn Val Ser Val Ser Arg Phe Asn Pro Ser Thr Trp Asn Lys Arg Phe 
    450                 455                 460                 


Gly Phe Ile Glu Asn Ser Val Phe Lys Pro Gln Pro Ala Gly Val Leu 
465                 470                 475                 480 


Thr Asn His Asp Val Val Tyr Ala Gln His Cys Phe Lys Ala Pro Lys 
                485                 490                 495     


Asn Phe Cys Pro Cys Lys Leu Asn Ser Ser Leu Cys Val Gly Ser Gly 
            500                 505                 510         


Pro Gly Lys Asn Asn Gly Ile Gly Thr Cys Pro Ala Gly Thr Asn Tyr 
        515                 520                 525             


Leu Thr Cys His Asn Leu Cys Asn Pro Asp Pro Ile Thr Phe Thr Gly 
    530                 535                 540                 


Pro Tyr Lys Cys Pro Gln Thr Lys Ser Leu Val Gly Ile Gly Glu His 
545                 550                 555                 560 


Cys Ser Gly Leu Ala Val Lys Ser Asp Tyr Cys Gly Gly Asn Pro Cys 
                565                 570                 575     


Thr Cys Gln Pro Gln Ala Phe Leu Gly Trp Ser Ala Asp Ser Cys Leu 
            580                 585                 590         


Gln Gly Asp Lys Cys Asn Ile Phe Ala Asn Leu Ile Leu His Asp Val 
        595                 600                 605             


Asn Ser Gly Leu Thr Cys Ser Thr Asp Leu Gln Lys Ala Asn Thr Asp 
    610                 615                 620                 


Ile Lys Leu Gly Val Cys Val Asn Tyr Asp Leu Tyr Gly Ile Ser Gly 
625                 630                 635                 640 


Gln Gly Ile Phe Val Glu Val Asn Ala Thr Tyr Tyr Asn Ser Trp Gln 
                645                 650                 655     


Asn Leu Leu Tyr Asp Ser Asn Gly Asn Leu Tyr Gly Phe Arg Asp Tyr 
            660                 665                 670         


Ile Thr Asn Arg Thr Phe Met Ile Arg Ser Cys Tyr Ser Gly Arg Val 
        675                 680                 685             


Ser Ala Ala Phe His Ala Asn Ser Ser Glu Pro Ala Leu Leu Phe Arg 
    690                 695                 700                 


Asn Ile Lys Cys Asn Tyr Val Phe Asn Asn Ser Leu Ile Arg Gln Leu 
705                 710                 715                 720 


Gln Pro Ile Asn Tyr Phe Asp Ser Tyr Leu Gly Cys Val Val Asn Ala 
                725                 730                 735     


Tyr Asn Ser Thr Ala Ile Ser Val Gln Thr Cys Asp Leu Thr Val Gly 
            740                 745                 750         


Ser Gly Tyr Cys Val Asp Tyr Ser Lys Asn Gly Gly Ser Gly Ser Ala 
        755                 760                 765             


Ile Thr Thr Gly Tyr Arg Phe Thr Asn Phe Glu Pro Phe Thr Val Asn 
    770                 775                 780                 


Ser Val Asn Asp Ser Leu Glu Pro Val Gly Gly Leu Tyr Glu Ile Gln 
785                 790                 795                 800 


Ile Pro Ser Glu Phe Thr Ile Gly Asn Met Glu Glu Phe Ile Gln Thr 
                805                 810                 815     


Ser Ser Pro Lys Val Thr Ile Asp Cys Ala Ala Phe Val Cys Gly Asp 
            820                 825                 830         


Tyr Ala Ala Cys Lys Ser Gln Leu Val Glu Tyr Gly Ser Phe Cys Asp 
        835                 840                 845             


Asn Ile Asn Ala Ile Leu Thr Glu Val Asn Glu Leu Leu Asp Thr Thr 
    850                 855                 860                 


Gln Leu Gln Val Ala Asn Ser Leu Met Asn Gly Val Thr Leu Ser Thr 
865                 870                 875                 880 


Lys Leu Lys Asp Gly Val Asn Phe Asn Val Asp Asp Ile Asn Phe Ser 
                885                 890                 895     


Ser Val Leu Gly Cys Leu Gly Ser Glu Cys Ser Lys Ala Ser Ser Arg 
            900                 905                 910         


Ser Ala Ile Glu Asp Leu Leu Phe Asp Lys Val Lys Leu Ser Asp Val 
        915                 920                 925             


Gly Phe Val Ala Ala Tyr Asn Asn Cys Thr Gly Gly Ala Glu Ile Arg 
    930                 935                 940                 


Asp Leu Ile Cys Val Gln Ser Tyr Lys Gly Ile Lys Val Leu Pro Pro 
945                 950                 955                 960 


Leu Leu Ser Glu Asn Gln Ile Ser Gly Tyr Thr Leu Ala Ala Thr Ser 
                965                 970                 975     


Ala Ser Leu Phe Pro Pro Trp Thr Ala Ala Ala Gly Val Pro Phe Tyr 
            980                 985                 990         


Leu Asn Val Gln Tyr Arg Ile Asn  Gly Leu Gly Val Thr  Met Asp Val 
        995                 1000                 1005             


Leu Ser  Gln Asn Gln Lys Leu  Ile Ala Asn Ala Phe  Asn Asn Ala 
    1010                 1015                 1020             


Leu Asp  Ala Ile Gln Glu Gly  Phe Asp Ala Thr Asn  Ser Ala Leu 
    1025                 1030                 1035             


Val Lys  Ile Gln Ala Val Val  Asn Ala Asn Ala Glu  Ala Leu Asn 
    1040                 1045                 1050             


Asn Leu  Leu Gln Gln Leu Ser  Asn Arg Phe Gly Ala  Ile Ser Ser 
    1055                 1060                 1065             


Ser Leu  Gln Glu Ile Leu Ser  Arg Leu Asp Pro Pro  Glu Ala Glu 
    1070                 1075                 1080             


Ala Gln  Ile Asp Arg Leu Ile  Asn Gly Arg Leu Thr  Ala Leu Asn 
    1085                 1090                 1095             


Ala Tyr  Val Ser Gln Gln Leu  Ser Asp Ser Thr Leu  Val Lys Phe 
    1100                 1105                 1110             


Ser Ala  Ala Gln Ala Met Glu  Lys Val Asn Glu Cys  Val Lys Ser 
    1115                 1120                 1125             


Gln Ser  Ser Arg Ile Asn Phe  Cys Gly Asn Gly Asn  His Ile Ile 
    1130                 1135                 1140             


Ser Leu  Val Gln Asn Ala Pro  Tyr Gly Leu Tyr Phe  Ile His Phe 
    1145                 1150                 1155             


Ser Tyr  Val Pro Thr Lys Tyr  Val Thr Ala Lys Val  Ser Pro Gly 
    1160                 1165                 1170             


Leu Cys  Ile Ala Gly Asp Arg  Gly Ile Ala Pro Lys  Ser Gly Tyr 
    1175                 1180                 1185             


Phe Val  Asn Val Asn Asn Thr  Trp Met Tyr Thr Gly  Ser Gly Tyr 
    1190                 1195                 1200             


Tyr Tyr  Pro Glu Pro Ile Thr  Glu Asn Asn Val Val  Val Met Ser 
    1205                 1210                 1215             


Thr Cys  Ala Val Asn Tyr Thr  Lys Ala Pro Tyr Val  Met Leu Asn 
    1220                 1225                 1230             


Thr Ser  Thr Pro Asn Leu Pro  Asp Phe Arg Glu Glu  Leu Asp Gln 
    1235                 1240                 1245             


Trp Phe  Lys Asn Gln Thr Ser  Val Ala Pro Asp Leu  Ser Leu Asp 
    1250                 1255                 1260             


Tyr Ile  Asn Val Thr Phe Leu  Asp Leu Gln Val Glu  Met Asn Arg 
    1265                 1270                 1275             


Leu Gln  Glu Ala Ile Lys Val  Leu Asn 
    1280                 1285         


<210>  12
<211>  1274
<212>  PRT
<213>  Rousettus bat coronavirus HKU9

<400>  12

Met Leu Leu Ile Leu Val Leu Gly Val Ser Leu Ala Ala Ala Ser Arg 
1               5                   10                  15      


Pro Glu Cys Phe Asn Pro Arg Phe Thr Leu Thr Pro Leu Asn His Thr 
            20                  25                  30          


Leu Asn Tyr Thr Ser Ile Lys Ala Lys Val Ser Asn Val Leu Leu Pro 
        35                  40                  45              


Asp Pro Tyr Ile Ala Tyr Ser Gly Gln Thr Leu Arg Gln Asn Leu Phe 
    50                  55                  60                  


Met Ala Asp Met Ser Asn Thr Ile Leu Tyr Pro Val Thr Pro Pro Ala 
65                  70                  75                  80  


Asn Gly Ala Asn Gly Gly Phe Ile Tyr Asn Thr Ser Ile Ile Pro Val 
                85                  90                  95      


Ser Ala Gly Leu Phe Val Asn Thr Trp Met Tyr Arg Gln Pro Ala Ser 
            100                 105                 110         


Ser Arg Ala Tyr Cys Gln Glu Pro Phe Gly Val Ala Phe Gly Asp Thr 
        115                 120                 125             


Phe Glu Asn Asp Arg Ile Ala Ile Leu Ile Met Ala Pro Asp Asn Leu 
    130                 135                 140                 


Gly Ser Trp Ser Ala Val Ala Pro Arg Asn Gln Thr Asn Ile Tyr Leu 
145                 150                 155                 160 


Leu Val Cys Ser Asn Ala Thr Leu Cys Ile Asn Pro Gly Phe Asn Arg 
                165                 170                 175     


Trp Gly Pro Ala Gly Ser Phe Ile Ala Pro Asp Ala Leu Val Asp His 
            180                 185                 190         


Ser Asn Ser Cys Phe Val Asn Asn Thr Phe Ser Val Asn Ile Ser Thr 
        195                 200                 205             


Ser Arg Ile Ser Leu Ala Phe Leu Phe Lys Asp Gly Asp Leu Leu Ile 
    210                 215                 220                 


Tyr His Ser Gly Trp Leu Pro Thr Ser Asn Phe Glu His Gly Phe Ser 
225                 230                 235                 240 


Arg Gly Ser His Pro Met Thr Tyr Phe Met Ser Leu Pro Val Gly Gly 
                245                 250                 255     


Asn Leu Pro Arg Ala Gln Phe Phe Gln Ser Ile Val Arg Ser Asn Ala 
            260                 265                 270         


Ile Asp Lys Gly Asp Gly Met Cys Thr Asn Phe Asp Val Asn Leu His 
        275                 280                 285             


Val Ala His Leu Ile Asn Arg Asp Leu Leu Val Ser Tyr Phe Asn Asn 
    290                 295                 300                 


Gly Ser Val Ala Asn Ala Ala Asp Cys Ala Asp Ser Ala Ala Glu Glu 
305                 310                 315                 320 


Leu Tyr Cys Val Thr Gly Ser Phe Asp Pro Pro Thr Gly Val Tyr Pro 
                325                 330                 335     


Leu Ser Arg Tyr Arg Ala Gln Val Ala Gly Phe Val Arg Val Thr Gln 
            340                 345                 350         


Arg Gly Ser Tyr Cys Thr Pro Pro Tyr Ser Val Leu Gln Asp Pro Pro 
        355                 360                 365             


Gln Pro Val Val Trp Arg Arg Tyr Met Leu Tyr Asp Cys Val Phe Asp 
    370                 375                 380                 


Phe Thr Val Val Val Asp Ser Leu Pro Thr His Gln Leu Gln Cys Tyr 
385                 390                 395                 400 


Gly Val Ser Pro Arg Arg Leu Ala Ser Met Cys Tyr Gly Ser Val Thr 
                405                 410                 415     


Leu Asp Val Met Arg Ile Asn Glu Thr His Leu Asn Asn Leu Phe Asn 
            420                 425                 430         


Arg Val Pro Asp Thr Phe Ser Leu Tyr Asn Tyr Ala Leu Pro Asp Asn 
        435                 440                 445             


Phe Tyr Gly Cys Leu His Ala Phe Tyr Leu Asn Ser Thr Ala Pro Tyr 
    450                 455                 460                 


Ala Val Ala Asn Arg Phe Pro Ile Lys Pro Gly Gly Arg Gln Ser Asn 
465                 470                 475                 480 


Ser Ala Phe Ile Asp Thr Val Ile Asn Ala Ala His Tyr Ser Pro Phe 
                485                 490                 495     


Ser Tyr Val Tyr Gly Leu Ala Val Ile Thr Leu Lys Pro Ala Ala Gly 
            500                 505                 510         


Ser Lys Leu Val Cys Pro Val Ala Asn Asp Thr Val Val Ile Thr Asp 
        515                 520                 525             


Arg Cys Val Gln Tyr Asn Leu Tyr Gly Tyr Thr Gly Thr Gly Val Leu 
    530                 535                 540                 


Ser Lys Asn Thr Ser Leu Val Ile Pro Asp Gly Lys Val Phe Thr Ala 
545                 550                 555                 560 


Ser Ser Thr Gly Thr Ile Ile Gly Val Ser Ile Asn Ser Thr Thr Tyr 
                565                 570                 575     


Ser Ile Met Pro Cys Val Thr Val Pro Val Ser Val Gly Tyr His Pro 
            580                 585                 590         


Asn Phe Glu Arg Ala Leu Leu Phe Asn Gly Leu Ser Cys Ser Gln Arg 
        595                 600                 605             


Ser Arg Ala Val Thr Glu Pro Val Ser Val Leu Trp Ser Ala Ser Ala 
    610                 615                 620                 


Thr Ala Gln Asp Ala Phe Asp Thr Pro Ser Gly Cys Val Val Asn Val 
625                 630                 635                 640 


Glu Leu Arg Asn Thr Thr Ile Val Asn Thr Cys Ala Met Pro Ile Gly 
                645                 650                 655     


Asn Ser Leu Cys Phe Ile Asn Gly Ser Ile Ala Thr Ala Asn Ala Asp 
            660                 665                 670         


Ser Leu Pro Arg Leu Gln Leu Val Asn Tyr Asp Pro Leu Tyr Asp Asn 
        675                 680                 685             


Ser Thr Ala Thr Pro Met Thr Pro Val Tyr Trp Val Lys Val Pro Thr 
    690                 695                 700                 


Asn Phe Thr Leu Ser Ala Thr Glu Glu Tyr Ile Gln Thr Thr Ala Pro 
705                 710                 715                 720 


Lys Ile Thr Ile Asp Cys Ala Arg Tyr Leu Cys Gly Asp Ser Ser Arg 
                725                 730                 735     


Cys Leu Asn Val Leu Leu His Tyr Gly Thr Phe Cys Asn Asp Ile Asn 
            740                 745                 750         


Lys Ala Leu Ser Arg Val Ser Thr Ile Leu Asp Ser Ala Leu Leu Ser 
        755                 760                 765             


Leu Val Lys Glu Leu Ser Ile Asn Thr Arg Asp Glu Val Thr Thr Phe 
    770                 775                 780                 


Ser Phe Asp Gly Asp Tyr Asn Phe Thr Gly Leu Met Gly Cys Leu Gly 
785                 790                 795                 800 


Pro Asn Cys Gly Ala Thr Thr Tyr Arg Ser Ala Phe Ser Asp Leu Leu 
                805                 810                 815     


Tyr Asp Lys Val Arg Ile Thr Asp Pro Gly Phe Met Gln Ser Tyr Gln 
            820                 825                 830         


Lys Cys Ile Asp Ser Gln Trp Gly Gly Ser Ile Arg Asp Leu Leu Cys 
        835                 840                 845             


Thr Gln Thr Tyr Asn Gly Ile Ala Val Leu Pro Pro Ile Val Ser Pro 
    850                 855                 860                 


Ala Met Gln Ala Leu Tyr Thr Ser Leu Leu Val Gly Ala Val Ala Ser 
865                 870                 875                 880 


Ser Gly Tyr Thr Phe Gly Ile Thr Ser Ala Gly Val Ile Pro Phe Ala 
                885                 890                 895     


Thr Gln Leu Gln Phe Arg Leu Asn Gly Ile Gly Val Thr Thr Gln Val 
            900                 905                 910         


Leu Val Glu Asn Gln Lys Leu Ile Ala Ser Ser Phe Asn Asn Ala Leu 
        915                 920                 925             


Val Asn Ile Gln Lys Gly Phe Thr Glu Thr Ser Ile Ala Leu Ser Lys 
    930                 935                 940                 


Met Gln Asp Val Ile Asn Gln His Ala Ala Gln Leu His Thr Leu Val 
945                 950                 955                 960 


Val Gln Leu Gly Asn Ser Phe Gly Ala Ile Ser Ser Ser Ile Asn Glu 
                965                 970                 975     


Ile Phe Ser Arg Leu Glu Gly Leu Ala Ala Asn Ala Glu Val Asp Arg 
            980                 985                 990         


Leu Ile Asn Gly Arg Met Met Val  Leu Asn Thr Tyr Val  Thr Gln Leu 
        995                 1000                 1005             


Leu Ile  Gln Ala Ser Glu Ala  Lys Ala Gln Asn Ala  Leu Ala Ala 
    1010                 1015                 1020             


Gln Lys  Ile Ser Glu Cys Val  Lys Ala Gln Ser Leu  Arg Asn Asp 
    1025                 1030                 1035             


Phe Cys  Gly Asn Gly Thr His  Val Leu Ser Ile Pro  Gln Leu Ala 
    1040                 1045                 1050             


Pro Asn  Gly Val Leu Phe Ile  His Tyr Ala Tyr Thr  Pro Thr Glu 
    1055                 1060                 1065             


Tyr Ala  Phe Val Gln Thr Ser  Ala Gly Leu Cys His  Asn Gly Thr 
    1070                 1075                 1080             


Gly Tyr  Ala Pro Arg Gln Gly  Met Phe Val Leu Pro  Asn Asn Thr 
    1085                 1090                 1095             


Asn Met  Trp His Phe Thr Thr  Met Gln Phe Tyr Asn  Pro Val Asn 
    1100                 1105                 1110             


Ile Ser  Ala Ser Asn Thr Gln  Val Leu Thr Ser Cys  Ser Val Asn 
    1115                 1120                 1125             


Tyr Thr  Ser Val Asn Tyr Thr  Val Leu Glu Pro Ser  Val Pro Gly 
    1130                 1135                 1140             


Asp Tyr  Asp Phe Gln Lys Glu  Phe Asp Lys Phe Tyr  Lys Asn Leu 
    1145                 1150                 1155             


Ser Thr  Ile Phe Asn Asn Thr  Phe Asn Pro Asn Asp  Phe Asn Phe 
    1160                 1165                 1170             


Ser Thr  Val Asp Val Thr Ala  Gln Ile Lys Ser Leu  His Asp Val 
    1175                 1180                 1185             


Val Asn  Gln Leu Asn Gln Ser  Phe Ile Asp Leu Lys  Lys Leu Asn 
    1190                 1195                 1200             


Val Tyr  Glu Lys Thr Ile Lys  Trp Pro Trp Tyr Val  Trp Leu Ala 
    1205                 1210                 1215             


Met Ile  Ala Gly Ile Val Gly  Leu Val Leu Ala Val  Ile Met Leu 
    1220                 1225                 1230             


Met Cys  Met Thr Asn Cys Cys  Ser Cys Phe Lys Gly  Met Cys Asp 
    1235                 1240                 1245             


Cys Arg  Arg Cys Cys Gly Ser  Tyr Asp Ser Tyr Asp  Asp Val Tyr 
    1250                 1255                 1260             


Pro Ala  Val Arg Val Asn Lys  Lys Arg Thr Val 
    1265                 1270                 


<210>  13
<211>  1207
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  13

Met Leu Leu Ile Leu Val Leu Gly Val Ser Leu Ala Ala Ala Ser Arg 
1               5                   10                  15      


Pro Glu Cys Phe Asn Pro Arg Phe Thr Leu Thr Pro Leu Asn His Thr 
            20                  25                  30          


Leu Asn Tyr Thr Ser Ile Lys Ala Lys Val Ser Asn Val Leu Leu Pro 
        35                  40                  45              


Asp Pro Tyr Ile Ala Tyr Ser Gly Gln Thr Leu Arg Gln Asn Leu Phe 
    50                  55                  60                  


Met Ala Asp Met Ser Asn Thr Ile Leu Tyr Pro Val Thr Pro Pro Ala 
65                  70                  75                  80  


Asn Gly Ala Asn Gly Gly Phe Ile Tyr Asn Thr Ser Ile Ile Pro Val 
                85                  90                  95      


Ser Ala Gly Leu Phe Val Asn Thr Trp Met Tyr Arg Gln Pro Ala Ser 
            100                 105                 110         


Ser Arg Ala Tyr Cys Gln Glu Pro Phe Gly Val Ala Phe Gly Asp Thr 
        115                 120                 125             


Phe Glu Asn Asp Arg Ile Ala Ile Leu Ile Met Ala Pro Asp Asn Leu 
    130                 135                 140                 


Gly Ser Trp Ser Ala Val Ala Pro Arg Asn Gln Thr Asn Ile Tyr Leu 
145                 150                 155                 160 


Leu Val Cys Ser Asn Ala Thr Leu Cys Ile Asn Pro Gly Phe Asn Arg 
                165                 170                 175     


Trp Gly Pro Ala Gly Ser Phe Ile Ala Pro Asp Ala Leu Val Asp His 
            180                 185                 190         


Ser Asn Ser Cys Phe Val Asn Asn Thr Phe Ser Val Asn Ile Ser Thr 
        195                 200                 205             


Ser Arg Ile Ser Leu Ala Phe Leu Phe Lys Asp Gly Asp Leu Leu Ile 
    210                 215                 220                 


Tyr His Ser Gly Trp Leu Pro Thr Ser Asn Phe Glu His Gly Phe Ser 
225                 230                 235                 240 


Arg Gly Ser His Pro Met Thr Tyr Phe Met Ser Leu Pro Val Gly Gly 
                245                 250                 255     


Asn Leu Pro Arg Ala Gln Phe Phe Gln Ser Ile Val Arg Ser Asn Ala 
            260                 265                 270         


Ile Asp Lys Gly Asp Gly Met Cys Thr Asn Phe Asp Val Asn Leu His 
        275                 280                 285             


Val Ala His Leu Ile Asn Arg Asp Leu Leu Val Ser Tyr Phe Asn Asn 
    290                 295                 300                 


Gly Ser Val Ala Asn Ala Ala Asp Cys Ala Asp Ser Ala Ala Glu Glu 
305                 310                 315                 320 


Leu Tyr Cys Val Thr Gly Ser Phe Asp Pro Pro Thr Gly Val Tyr Pro 
                325                 330                 335     


Leu Ser Arg Tyr Arg Ala Gln Val Ala Gly Phe Val Arg Val Thr Gln 
            340                 345                 350         


Arg Gly Ser Tyr Cys Thr Pro Pro Tyr Ser Val Leu Gln Asp Pro Pro 
        355                 360                 365             


Gln Pro Val Val Trp Arg Arg Tyr Met Leu Tyr Asp Cys Val Phe Asp 
    370                 375                 380                 


Phe Thr Val Val Val Asp Ser Leu Pro Thr His Gln Leu Gln Cys Tyr 
385                 390                 395                 400 


Gly Val Ser Pro Arg Arg Leu Ala Ser Met Cys Tyr Gly Ser Val Thr 
                405                 410                 415     


Leu Asp Val Met Arg Ile Asn Glu Thr His Leu Asn Asn Leu Phe Asn 
            420                 425                 430         


Arg Val Pro Asp Thr Phe Ser Leu Tyr Asn Tyr Ala Leu Pro Asp Asn 
        435                 440                 445             


Phe Tyr Gly Cys Leu His Ala Phe Tyr Leu Asn Ser Thr Ala Pro Tyr 
    450                 455                 460                 


Ala Val Ala Asn Arg Phe Pro Ile Lys Pro Gly Gly Arg Gln Ser Asn 
465                 470                 475                 480 


Ser Ala Phe Ile Asp Thr Val Ile Asn Ala Ala His Tyr Ser Pro Phe 
                485                 490                 495     


Ser Tyr Val Tyr Gly Leu Ala Val Ile Thr Leu Lys Pro Ala Ala Gly 
            500                 505                 510         


Ser Lys Leu Val Cys Pro Val Ala Asn Asp Thr Val Val Ile Thr Asp 
        515                 520                 525             


Arg Cys Val Gln Tyr Asn Leu Tyr Gly Tyr Thr Gly Thr Gly Val Leu 
    530                 535                 540                 


Ser Lys Asn Thr Ser Leu Val Ile Pro Asp Gly Lys Val Phe Thr Ala 
545                 550                 555                 560 


Ser Ser Thr Gly Thr Ile Ile Gly Val Ser Ile Asn Ser Thr Thr Tyr 
                565                 570                 575     


Ser Ile Met Pro Cys Val Thr Val Pro Val Ser Val Gly Tyr His Pro 
            580                 585                 590         


Asn Phe Glu Arg Ala Leu Leu Phe Asn Gly Leu Ser Cys Ser Gln Arg 
        595                 600                 605             


Ser Arg Ala Val Thr Glu Pro Val Ser Val Leu Trp Ser Ala Ser Ala 
    610                 615                 620                 


Thr Ala Gln Asp Ala Phe Asp Thr Pro Ser Gly Cys Val Val Asn Val 
625                 630                 635                 640 


Glu Leu Arg Asn Thr Thr Ile Val Asn Thr Cys Ala Met Pro Ile Gly 
                645                 650                 655     


Asn Ser Leu Cys Phe Ile Asn Gly Ser Ile Ala Thr Ala Asn Ala Asp 
            660                 665                 670         


Ser Leu Pro Arg Leu Gln Leu Val Asn Tyr Asp Pro Leu Tyr Asp Asn 
        675                 680                 685             


Ser Thr Ala Thr Pro Met Thr Pro Val Tyr Trp Val Lys Val Pro Thr 
    690                 695                 700                 


Asn Phe Thr Leu Ser Ala Thr Glu Glu Tyr Ile Gln Thr Thr Ala Pro 
705                 710                 715                 720 


Lys Ile Thr Ile Asp Cys Ala Arg Tyr Leu Cys Gly Asp Ser Ser Arg 
                725                 730                 735     


Cys Leu Asn Val Leu Leu His Tyr Gly Thr Phe Cys Asn Asp Ile Asn 
            740                 745                 750         


Lys Ala Leu Ser Arg Val Ser Thr Ile Leu Asp Ser Ala Leu Leu Ser 
        755                 760                 765             


Leu Val Lys Glu Leu Ser Ile Asn Thr Arg Asp Glu Val Thr Thr Phe 
    770                 775                 780                 


Ser Phe Asp Gly Asp Tyr Asn Phe Thr Gly Leu Met Gly Cys Leu Gly 
785                 790                 795                 800 


Pro Asn Cys Gly Ala Thr Thr Tyr Arg Ser Ala Phe Ser Asp Leu Leu 
                805                 810                 815     


Tyr Asp Lys Val Arg Ile Thr Asp Pro Gly Phe Met Gln Ser Tyr Gln 
            820                 825                 830         


Lys Cys Ile Asp Ser Gln Trp Gly Gly Ser Ile Arg Asp Leu Leu Cys 
        835                 840                 845             


Thr Gln Thr Tyr Asn Gly Ile Ala Val Leu Pro Pro Ile Val Ser Pro 
    850                 855                 860                 


Ala Met Gln Ala Leu Tyr Thr Ser Leu Leu Val Gly Ala Val Ala Ser 
865                 870                 875                 880 


Ser Gly Tyr Thr Phe Gly Ile Thr Ser Ala Gly Val Ile Pro Phe Ala 
                885                 890                 895     


Thr Gln Leu Gln Phe Arg Leu Asn Gly Ile Gly Val Thr Thr Gln Val 
            900                 905                 910         


Leu Val Glu Asn Gln Lys Leu Ile Ala Ser Ser Phe Asn Asn Ala Leu 
        915                 920                 925             


Val Asn Ile Gln Lys Gly Phe Thr Glu Thr Ser Ile Ala Leu Ser Lys 
    930                 935                 940                 


Met Gln Asp Val Ile Asn Gln His Ala Ala Gln Leu His Thr Leu Val 
945                 950                 955                 960 


Val Gln Leu Gly Asn Ser Phe Gly Ala Ile Ser Ser Ser Ile Asn Glu 
                965                 970                 975     


Ile Phe Ser Arg Leu Glu Pro Pro Ala Ala Asn Ala Glu Val Asp Arg 
            980                 985                 990         


Leu Ile Asn Gly Arg Met Met Val  Leu Asn Thr Tyr Val  Thr Gln Leu 
        995                 1000                 1005             


Leu Ile  Gln Ala Ser Glu Ala  Lys Ala Gln Asn Ala  Leu Ala Ala 
    1010                 1015                 1020             


Gln Lys  Ile Ser Glu Cys Val  Lys Ala Gln Ser Leu  Arg Asn Asp 
    1025                 1030                 1035             


Phe Cys  Gly Asn Gly Thr His  Val Leu Ser Ile Pro  Gln Leu Ala 
    1040                 1045                 1050             


Pro Asn  Gly Val Leu Phe Ile  His Tyr Ala Tyr Thr  Pro Thr Glu 
    1055                 1060                 1065             


Tyr Ala  Phe Val Gln Thr Ser  Ala Gly Leu Cys His  Asn Gly Thr 
    1070                 1075                 1080             


Gly Tyr  Ala Pro Arg Gln Gly  Met Phe Val Leu Pro  Asn Asn Thr 
    1085                 1090                 1095             


Asn Met  Trp His Phe Thr Thr  Met Gln Phe Tyr Asn  Pro Val Asn 
    1100                 1105                 1110             


Ile Ser  Ala Ser Asn Thr Gln  Val Leu Thr Ser Cys  Ser Val Asn 
    1115                 1120                 1125             


Tyr Thr  Ser Val Asn Tyr Thr  Val Leu Glu Pro Ser  Val Pro Gly 
    1130                 1135                 1140             


Asp Tyr  Asp Phe Gln Lys Glu  Phe Asp Lys Phe Tyr  Lys Asn Leu 
    1145                 1150                 1155             


Ser Thr  Ile Phe Asn Asn Thr  Phe Asn Pro Asn Asp  Phe Asn Phe 
    1160                 1165                 1170             


Ser Thr  Val Asp Val Thr Ala  Gln Ile Lys Ser Leu  His Asp Val 
    1175                 1180                 1185             


Val Asn  Gln Leu Asn Gln Ser  Phe Ile Asp Leu Lys  Lys Leu Asn 
    1190                 1195                 1200             


Val Tyr  Glu Lys 
    1205         


<210>  14
<211>  1256
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  14

Met Lys Leu Leu Val Leu Val Phe Ala Thr Leu Val Ser Ser Tyr Thr 
1               5                   10                  15      


Ile Glu Lys Cys Leu Asp Phe Asp Asp Arg Thr Pro Pro Ala Asn Thr 
            20                  25                  30          


Gln Phe Leu Ser Ser His Arg Gly Val Tyr Tyr Pro Asp Asp Ile Phe 
        35                  40                  45              


Arg Ser Asn Val Leu His Leu Val Gln Asp His Phe Leu Pro Phe Asp 
    50                  55                  60                  


Ser Asn Val Thr Arg Phe Ile Thr Phe Gly Leu Asn Phe Asp Asn Pro 
65                  70                  75                  80  


Ile Ile Pro Phe Lys Asp Gly Ile Tyr Phe Ala Ala Thr Glu Lys Ser 
                85                  90                  95      


Asn Val Ile Arg Gly Trp Val Phe Gly Ser Thr Met Asn Asn Lys Ser 
            100                 105                 110         


Gln Ser Val Ile Ile Met Asn Asn Ser Thr Asn Leu Val Ile Arg Ala 
        115                 120                 125             


Cys Asn Phe Glu Leu Cys Asp Asn Pro Phe Phe Val Val Leu Lys Ser 
    130                 135                 140                 


Asn Asn Thr Gln Ile Pro Ser Tyr Ile Phe Asn Asn Ala Phe Asn Cys 
145                 150                 155                 160 


Thr Phe Glu Tyr Val Ser Lys Asp Phe Asn Leu Asp Leu Gly Glu Lys 
                165                 170                 175     


Pro Gly Asn Phe Lys Asp Leu Arg Glu Phe Val Phe Arg Asn Lys Asp 
            180                 185                 190         


Gly Phe Leu His Val Tyr Ser Gly Tyr Gln Pro Ile Ser Ala Ala Ser 
        195                 200                 205             


Gly Leu Pro Thr Gly Phe Asn Ala Leu Lys Pro Ile Phe Lys Leu Pro 
    210                 215                 220                 


Leu Gly Ile Asn Ile Thr Asn Phe Arg Thr Leu Leu Thr Ala Phe Pro 
225                 230                 235                 240 


Pro Arg Pro Asp Tyr Trp Gly Thr Ser Ala Ala Ala Tyr Phe Val Gly 
                245                 250                 255     


Tyr Leu Lys Pro Thr Thr Phe Met Leu Lys Tyr Asp Glu Asn Gly Thr 
            260                 265                 270         


Ile Thr Asp Ala Val Asp Cys Ser Gln Asn Pro Leu Ala Glu Leu Lys 
        275                 280                 285             


Cys Ser Val Lys Ser Phe Glu Ile Asp Lys Gly Ile Tyr Gln Thr Ser 
    290                 295                 300                 


Asn Phe Arg Val Ala Pro Ser Lys Glu Val Val Arg Phe Pro Asn Ile 
305                 310                 315                 320 


Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Thr Phe Pro 
                325                 330                 335     


Ser Val Tyr Ala Trp Glu Arg Lys Arg Ile Ser Asn Cys Val Ala Asp 
            340                 345                 350         


Tyr Ser Val Leu Tyr Asn Ser Thr Ser Phe Ser Thr Phe Lys Cys Tyr 
        355                 360                 365             


Gly Val Ser Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr 
    370                 375                 380                 


Ala Asp Ser Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro 
385                 390                 395                 400 


Gly Gln Thr Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp 
                405                 410                 415     


Phe Thr Gly Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr 
            420                 425                 430         


Gln Thr Gly Asn Tyr Asn Tyr Lys Tyr Arg Ser Leu Arg His Gly Lys 
        435                 440                 445             


Leu Arg Pro Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp 
    450                 455                 460                 


Gly Lys Pro Cys Thr Pro Pro Ala Phe Asn Cys Tyr Trp Pro Leu Asn 
465                 470                 475                 480 


Asp Tyr Gly Phe Tyr Ile Thr Asn Gly Ile Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Leu Ser Thr Asp Leu Ile Lys Asn Gln Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Pro Ser Ser Lys 
    530                 535                 540                 


Arg Phe Gln Pro Phe Gln Gln Phe Gly Arg Asp Val Ser Asp Phe Thr 
545                 550                 555                 560 


Asp Ser Val Arg Asp Pro Lys Thr Ser Glu Ile Leu Asp Ile Ser Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Ser Glu Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Asp Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Ser Trp Arg Val His Ser 
    610                 615                 620                 


Thr Gly Asn Asn Val Phe Gln Thr Gln Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asp Thr Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr His Thr Val Ser Ser Leu Arg Ser Thr Ser Gln 
            660                 665                 670         


Lys Ser Ile Val Ala Tyr Thr Met Ser Leu Gly Ala Asp Ser Ser Ile 
        675                 680                 685             


Ala Tyr Ser Asn Asn Thr Ile Ala Ile Pro Thr Asn Phe Ser Ile Ser 
    690                 695                 700                 


Ile Thr Thr Glu Val Met Pro Val Ser Met Ala Lys Thr Ser Val Asp 
705                 710                 715                 720 


Cys Asn Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ala Asn Leu Leu 
                725                 730                 735     


Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Ser Gly 
            740                 745                 750         


Ile Ala Val Glu Gln Asp Arg Asn Thr Arg Glu Val Phe Ala Gln Val 
        755                 760                 765             


Lys Gln Met Tyr Lys Thr Pro Thr Leu Lys Asp Phe Gly Gly Phe Asn 
    770                 775                 780                 


Phe Ser Gln Ile Leu Pro Asp Pro Leu Lys Pro Thr Lys Arg Ser Phe 
785                 790                 795                 800 


Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe 
                805                 810                 815     


Met Lys Gln Tyr Gly Glu Cys Leu Gly Asp Ile Asn Ala Arg Asp Leu 
            820                 825                 830         


Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu 
        835                 840                 845             


Thr Asp Asp Met Ile Ala Ala Tyr Thr Ala Ala Leu Val Ser Gly Thr 
    850                 855                 860                 


Ala Thr Ala Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro 
865                 870                 875                 880 


Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln 
                885                 890                 895     


Asn Val Leu Tyr Glu Asn Gln Lys Gln Ile Ala Asn Gln Phe Asn Lys 
            900                 905                 910         


Ala Ile Ser Gln Ile Gln Glu Ser Leu Thr Thr Thr Ser Thr Ala Leu 
        915                 920                 925             


Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr 
    930                 935                 940                 


Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu 
945                 950                 955                 960 


Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile 
                965                 970                 975     


Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr 
            980                 985                 990         


Gln Gln Leu Ile Arg Ala Ala Glu  Ile Arg Ala Ser Ala  Asn Leu Ala 
        995                 1000                 1005             


Ala Thr  Lys Met Ser Glu Cys  Val Leu Gly Gln Ser  Lys Arg Val 
    1010                 1015                 1020             


Asp Phe  Cys Gly Lys Gly Tyr  His Leu Met Ser Phe  Pro Gln Ala 
    1025                 1030                 1035             


Ala Pro  His Gly Val Val Phe  Leu His Val Thr Tyr  Val Pro Ser 
    1040                 1045                 1050             


Gln Glu  Arg Asn Phe Thr Thr  Ala Pro Ala Ile Cys  His Glu Gly 
    1055                 1060                 1065             


Lys Ala  Tyr Phe Pro Arg Glu  Gly Val Phe Val Phe  Asn Gly Thr 
    1070                 1075                 1080             


Ser Trp  Phe Ile Thr Gln Arg  Asn Phe Phe Ser Pro  Gln Ile Ile 
    1085                 1090                 1095             


Thr Thr  Asp Asn Thr Phe Val  Ser Gly Ser Cys Asp  Val Val Ile 
    1100                 1105                 1110             


Gly Ile  Ile Asn Asn Thr Val  Tyr Asp Pro Leu Gln  Pro Glu Leu 
    1115                 1120                 1125             


Asp Ser  Phe Lys Glu Glu Leu  Asp Lys Tyr Phe Lys  Asn His Thr 
    1130                 1135                 1140             


Ser Pro  Asp Val Asp Leu Gly  Asp Ile Ser Gly Ile  Asn Ala Ser 
    1145                 1150                 1155             


Val Val  Asn Ile Gln Lys Glu  Ile Asp Arg Leu Asn  Glu Val Ala 
    1160                 1165                 1170             


Lys Asn  Leu Asn Glu Ser Leu  Ile Asp Leu Gln Glu  Leu Gly Lys 
    1175                 1180                 1185             


Tyr Glu  Gln Tyr Ile Lys Trp  Pro Trp Tyr Val Trp  Leu Gly Phe 
    1190                 1195                 1200             


Ile Ala  Gly Leu Ile Ala Ile  Val Met Val Thr Ile  Leu Leu Cys 
    1205                 1210                 1215             


Cys Met  Thr Ser Cys Cys Ser  Cys Leu Lys Gly Ala  Cys Ser Cys 
    1220                 1225                 1230             


Gly Ser  Cys Cys Lys Phe Asp  Glu Asp Asp Ser Glu  Pro Val Leu 
    1235                 1240                 1245             


Lys Gly  Val Lys Leu His Tyr  Thr 
    1250                 1255     


<210>  15
<211>  1191
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  15

Met Lys Leu Leu Val Leu Val Phe Ala Thr Leu Val Ser Ser Tyr Thr 
1               5                   10                  15      


Ile Glu Lys Cys Leu Asp Phe Asp Asp Arg Thr Pro Pro Ala Asn Thr 
            20                  25                  30          


Gln Phe Leu Ser Ser His Arg Gly Val Tyr Tyr Pro Asp Asp Ile Phe 
        35                  40                  45              


Arg Ser Asn Val Leu His Leu Val Gln Asp His Phe Leu Pro Phe Asp 
    50                  55                  60                  


Ser Asn Val Thr Arg Phe Ile Thr Phe Gly Leu Asn Phe Asp Asn Pro 
65                  70                  75                  80  


Ile Ile Pro Phe Lys Asp Gly Ile Tyr Phe Ala Ala Thr Glu Lys Ser 
                85                  90                  95      


Asn Val Ile Arg Gly Trp Val Phe Gly Ser Thr Met Asn Asn Lys Ser 
            100                 105                 110         


Gln Ser Val Ile Ile Met Asn Asn Ser Thr Asn Leu Val Ile Arg Ala 
        115                 120                 125             


Cys Asn Phe Glu Leu Cys Asp Asn Pro Phe Phe Val Val Leu Lys Ser 
    130                 135                 140                 


Asn Asn Thr Gln Ile Pro Ser Tyr Ile Phe Asn Asn Ala Phe Asn Cys 
145                 150                 155                 160 


Thr Phe Glu Tyr Val Ser Lys Asp Phe Asn Leu Asp Leu Gly Glu Lys 
                165                 170                 175     


Pro Gly Asn Phe Lys Asp Leu Arg Glu Phe Val Phe Arg Asn Lys Asp 
            180                 185                 190         


Gly Phe Leu His Val Tyr Ser Gly Tyr Gln Pro Ile Ser Ala Ala Ser 
        195                 200                 205             


Gly Leu Pro Thr Gly Phe Asn Ala Leu Lys Pro Ile Phe Lys Leu Pro 
    210                 215                 220                 


Leu Gly Ile Asn Ile Thr Asn Phe Arg Thr Leu Leu Thr Ala Phe Pro 
225                 230                 235                 240 


Pro Arg Pro Asp Tyr Trp Gly Thr Ser Ala Ala Ala Tyr Phe Val Gly 
                245                 250                 255     


Tyr Leu Lys Pro Thr Thr Phe Met Leu Lys Tyr Asp Glu Asn Gly Thr 
            260                 265                 270         


Ile Thr Asp Ala Val Asp Cys Ser Gln Asn Pro Leu Ala Glu Leu Lys 
        275                 280                 285             


Cys Ser Val Lys Ser Phe Glu Ile Asp Lys Gly Ile Tyr Gln Thr Ser 
    290                 295                 300                 


Asn Phe Arg Val Ala Pro Ser Lys Glu Val Val Arg Phe Pro Asn Ile 
305                 310                 315                 320 


Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Thr Phe Pro 
                325                 330                 335     


Ser Val Tyr Ala Trp Glu Arg Lys Arg Ile Ser Asn Cys Val Ala Asp 
            340                 345                 350         


Tyr Ser Val Leu Tyr Asn Ser Thr Ser Phe Ser Thr Phe Lys Cys Tyr 
        355                 360                 365             


Gly Val Ser Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr 
    370                 375                 380                 


Ala Asp Ser Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro 
385                 390                 395                 400 


Gly Gln Thr Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp 
                405                 410                 415     


Phe Thr Gly Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr 
            420                 425                 430         


Gln Thr Gly Asn Tyr Asn Tyr Lys Tyr Arg Ser Leu Arg His Gly Lys 
        435                 440                 445             


Leu Arg Pro Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp 
    450                 455                 460                 


Gly Lys Pro Cys Thr Pro Pro Ala Phe Asn Cys Tyr Trp Pro Leu Asn 
465                 470                 475                 480 


Asp Tyr Gly Phe Tyr Ile Thr Asn Gly Ile Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Leu Ser Thr Asp Leu Ile Lys Asn Gln Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Pro Ser Ser Lys 
    530                 535                 540                 


Arg Phe Gln Pro Phe Gln Gln Phe Gly Arg Asp Val Ser Asp Phe Thr 
545                 550                 555                 560 


Asp Ser Val Arg Asp Pro Lys Thr Ser Glu Ile Leu Asp Ile Ser Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Ser Glu Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Asp Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Ser Trp Arg Val His Ser 
    610                 615                 620                 


Thr Gly Asn Asn Val Phe Gln Thr Gln Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asp Thr Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr His Thr Val Ser Ser Leu Arg Ser Thr Ser Gln 
            660                 665                 670         


Lys Ser Ile Val Ala Tyr Thr Met Ser Leu Gly Ala Asp Ser Ser Ile 
        675                 680                 685             


Ala Tyr Ser Asn Asn Thr Ile Ala Ile Pro Thr Asn Phe Ser Ile Ser 
    690                 695                 700                 


Ile Thr Thr Glu Val Met Pro Val Ser Met Ala Lys Thr Ser Val Asp 
705                 710                 715                 720 


Cys Asn Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ala Asn Leu Leu 
                725                 730                 735     


Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Ser Gly 
            740                 745                 750         


Ile Ala Val Glu Gln Asp Arg Asn Thr Arg Glu Val Phe Ala Gln Val 
        755                 760                 765             


Lys Gln Met Tyr Lys Thr Pro Thr Leu Lys Asp Phe Gly Gly Phe Asn 
    770                 775                 780                 


Phe Ser Gln Ile Leu Pro Asp Pro Leu Lys Pro Thr Lys Arg Ser Phe 
785                 790                 795                 800 


Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe 
                805                 810                 815     


Met Lys Gln Tyr Gly Glu Cys Leu Gly Asp Ile Asn Ala Arg Asp Leu 
            820                 825                 830         


Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu 
        835                 840                 845             


Thr Asp Asp Met Ile Ala Ala Tyr Thr Ala Ala Leu Val Ser Gly Thr 
    850                 855                 860                 


Ala Thr Ala Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro 
865                 870                 875                 880 


Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln 
                885                 890                 895     


Asn Val Leu Tyr Glu Asn Gln Lys Gln Ile Ala Asn Gln Phe Asn Lys 
            900                 905                 910         


Ala Ile Ser Gln Ile Gln Glu Ser Leu Thr Thr Thr Ser Thr Ala Leu 
        915                 920                 925             


Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr 
    930                 935                 940                 


Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu 
945                 950                 955                 960 


Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile 
                965                 970                 975     


Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr 
            980                 985                 990         


Gln Gln Leu Ile Arg Ala Ala Glu  Ile Arg Ala Ser Ala  Asn Leu Ala 
        995                 1000                 1005             


Ala Thr  Lys Met Ser Glu Cys  Val Leu Gly Gln Ser  Lys Arg Val 
    1010                 1015                 1020             


Asp Phe  Cys Gly Lys Gly Tyr  His Leu Met Ser Phe  Pro Gln Ala 
    1025                 1030                 1035             


Ala Pro  His Gly Val Val Phe  Leu His Val Thr Tyr  Val Pro Ser 
    1040                 1045                 1050             


Gln Glu  Arg Asn Phe Thr Thr  Ala Pro Ala Ile Cys  His Glu Gly 
    1055                 1060                 1065             


Lys Ala  Tyr Phe Pro Arg Glu  Gly Val Phe Val Phe  Asn Gly Thr 
    1070                 1075                 1080             


Ser Trp  Phe Ile Thr Gln Arg  Asn Phe Phe Ser Pro  Gln Ile Ile 
    1085                 1090                 1095             


Thr Thr  Asp Asn Thr Phe Val  Ser Gly Ser Cys Asp  Val Val Ile 
    1100                 1105                 1110             


Gly Ile  Ile Asn Asn Thr Val  Tyr Asp Pro Leu Gln  Pro Glu Leu 
    1115                 1120                 1125             


Asp Ser  Phe Lys Glu Glu Leu  Asp Lys Tyr Phe Lys  Asn His Thr 
    1130                 1135                 1140             


Ser Pro  Asp Val Asp Leu Gly  Asp Ile Ser Gly Ile  Asn Ala Ser 
    1145                 1150                 1155             


Val Val  Asn Ile Gln Lys Glu  Ile Asp Arg Leu Asn  Glu Val Ala 
    1160                 1165                 1170             


Lys Asn  Leu Asn Glu Ser Leu  Ile Asp Leu Gln Glu  Leu Gly Lys 
    1175                 1180                 1185             


Tyr Glu  Gln 
    1190     


<210>  16
<211>  1361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  16

Met Leu Ser Val Phe Ile Leu Phe Leu Pro Ser Cys Leu Gly Tyr Ile 
1               5                   10                  15      


Gly Asp Phe Arg Cys Ile Asn Leu Val Asn Thr Asp Thr Ser Asn Ala 
            20                  25                  30          


Ser Ala Pro Ser Val Ser Thr Glu Val Val Asp Val Ser Lys Gly Ile 
        35                  40                  45              


Gly Thr Tyr Tyr Val Leu Asp Arg Val Tyr Leu Asn Ala Thr Leu Leu 
    50                  55                  60                  


Leu Thr Gly Tyr Tyr Pro Val Asp Gly Ser Asn Tyr Arg Asn Leu Ala 
65                  70                  75                  80  


Leu Thr Gly Thr Asn Thr Leu Ser Leu Asn Trp Tyr Lys Pro Pro Phe 
                85                  90                  95      


Leu Ser Glu Phe Asn Asp Gly Ile Phe Ala Lys Val Lys Asn Leu Lys 
            100                 105                 110         


Ala Ser Leu Pro Lys Asp Ser Thr Ser Tyr Phe Pro Thr Ile Val Ile 
        115                 120                 125             


Gly Ser Asn Phe Val Thr Thr Ser Tyr Thr Val Val Leu Glu Pro Tyr 
    130                 135                 140                 


Asn Gly Ile Ile Met Ala Ser Ile Cys Gln Tyr Thr Ile Cys Leu Leu 
145                 150                 155                 160 


Pro Tyr Thr Asp Cys Lys Pro Asn Thr Gly Gly Asn Lys Leu Ile Gly 
                165                 170                 175     


Phe Trp His Ile Asp Leu Lys Ser Pro Val Cys Ile Leu Lys Arg Asn 
            180                 185                 190         


Phe Thr Phe Asn Val Asn Ala Asp Trp Leu Tyr Phe His Phe Tyr Gln 
        195                 200                 205             


Gln Gly Gly Thr Phe Tyr Ala Tyr Tyr Ala Asp Ala Gly Ser Ala Thr 
    210                 215                 220                 


Thr Phe Leu Phe Ser Ser Tyr Ile Gly Asp Val Leu Thr Gln Tyr Phe 
225                 230                 235                 240 


Val Leu Pro Phe Val Cys Thr Pro Thr Thr Thr Gly Val Phe Ser Pro 
                245                 250                 255     


Gln Tyr Trp Val Thr Pro Leu Val Lys Arg Gln Tyr Leu Phe Asn Phe 
            260                 265                 270         


Asn Gln Lys Gly Thr Ile Thr Ser Ala Val Asp Cys Ala Ser Ser Tyr 
        275                 280                 285             


Thr Ser Glu Ile Lys Cys Lys Thr Gln Ser Met Asn Pro Asn Thr Gly 
    290                 295                 300                 


Val Tyr Asp Leu Ser Gly Tyr Thr Val Gln Pro Val Gly Leu Val Tyr 
305                 310                 315                 320 


Arg Arg Val Arg Asn Leu Pro Asp Cys Arg Ile Glu Asp Trp Leu Ala 
                325                 330                 335     


Ala Lys Thr Val Pro Ser Pro Leu Asn Trp Glu Arg Lys Thr Phe Gln 
            340                 345                 350         


Asn Cys Asn Phe Asn Leu Ser Ser Leu Leu Arg Leu Val Gln Ala Gly 
        355                 360                 365             


Ser Leu Ser Cys Ser Asn Ile Asp Ala Ala Lys Val Tyr Gly Met Cys 
    370                 375                 380                 


Phe Gly Ser Met Ser Ile Asp Lys Phe Ala Ile Pro Asn Ser Arg Arg 
385                 390                 395                 400 


Val Asp Leu Gln Leu Gly Asn Ser Gly Phe Leu Gln Ser Phe Asn Tyr 
                405                 410                 415     


Lys Ile Asp Thr Arg Ala Thr Ser Cys Gln Leu Tyr Tyr Ser Leu Ala 
            420                 425                 430         


Gln Ser Asn Val Thr Val Asn Asn His Asn Pro Ser Ser Trp Asn Arg 
        435                 440                 445             


Arg Tyr Gly Phe Asn Asp Val Ala Thr Phe Gly Arg Gly Lys His Asp 
    450                 455                 460                 


Val Ala Tyr Ala Glu Ala Cys Phe Thr Val Gly Ala Ser Tyr Cys Pro 
465                 470                 475                 480 


Cys Ala Asn Pro Ser Ile Val Ser Pro Cys Thr Thr Gly Lys Pro Lys 
                485                 490                 495     


Phe Ala Asn Cys Pro Thr Gly Thr Thr Asn Arg Glu Cys Asn Val Leu 
            500                 505                 510         


Ala Leu Gly Ser Asn Leu Phe Lys Cys Asp Cys Thr Cys Asn Pro Ser 
        515                 520                 525             


Pro Leu Thr Thr Tyr Asp Leu Arg Cys Leu Gln Gly Arg Ser Met Leu 
    530                 535                 540                 


Gly Val Gly Asp His Cys Glu Gly Leu Gly Val Leu Glu Asp Lys Cys 
545                 550                 555                 560 


Gly Gly Ser Asn Thr Cys Asn Cys Ser Ala Asp Ala Phe Val Gly Trp 
                565                 570                 575     


Ala Lys Asp Ser Cys Leu Ser Asn Gly Arg Cys His Ile Phe Ser Asn 
            580                 585                 590         


Leu Met Leu Asn Gly Ile Asn Ser Gly Thr Thr Cys Ser Thr Asp Leu 
        595                 600                 605             


Gln Leu Pro Asn Thr Glu Val Val Thr Gly Ile Cys Val Lys Tyr Asp 
    610                 615                 620                 


Leu Tyr Gly Ile Thr Gly Gln Gly Val Phe Lys Glu Val Lys Ala Asp 
625                 630                 635                 640 


Tyr Tyr Asn Ser Trp Gln Asn Leu Leu Tyr Asp Val Asn Gly Asn Leu 
                645                 650                 655     


Asn Gly Phe Arg Asp Ile Val Thr Asn Lys Thr Tyr Leu Thr Arg Ser 
            660                 665                 670         


Cys Tyr Ser Gly Arg Val Ser Ala Ala Tyr His Gln Asp Ala Pro Glu 
        675                 680                 685             


Pro Ala Leu Leu Tyr Arg Asn Leu Lys Cys Asp Tyr Val Phe Asn Asn 
    690                 695                 700                 


Asn Ile Phe Arg Glu Glu Thr Pro Leu Asn Tyr Phe Asp Ser Tyr Leu 
705                 710                 715                 720 


Gly Cys Val Val Asn Ala Asp Asn Ser Thr Glu Gln Ala Val Asp Ala 
                725                 730                 735     


Cys Asp Leu Arg Met Gly Ser Gly Leu Cys Val Asn Tyr Ser Thr Ala 
            740                 745                 750         


His Arg Ala Arg Thr Ser Val Ser Thr Gly Tyr Lys Leu Thr Thr Phe 
        755                 760                 765             


Glu Pro Phe Thr Val Ser Ile Val Asn Asp Ser Val Glu Ser Val Gly 
    770                 775                 780                 


Gly Leu Tyr Glu Met Gln Ile Pro Thr Asn Phe Thr Ile Ala Ser His 
785                 790                 795                 800 


Gln Glu Phe Ile Gln Thr Arg Ala Pro Lys Val Thr Ile Asp Cys Ala 
                805                 810                 815     


Ala Phe Val Cys Gly Asp Tyr Thr Thr Cys Arg Gln Gln Leu Val Glu 
            820                 825                 830         


Tyr Gly Ser Phe Cys Asp Asn Ile Asn Ala Ile Leu Gly Glu Val Asn 
        835                 840                 845             


Asn Leu Ile Asp Thr Met Gln Leu Gln Val Ala Ser Ala Leu Ile Gln 
    850                 855                 860                 


Gly Val Thr Leu Ser Ser Arg Leu Ala Asp Gly Ile Ser Gly Gln Ile 
865                 870                 875                 880 


Asp Asp Ile Asn Phe Ser Pro Leu Leu Gly Cys Leu Gly Ser Gln Cys 
                885                 890                 895     


Ser Glu Gly Thr Met Ala Ala Gln Gly Arg Ser Thr Val Glu Asp Leu 
            900                 905                 910         


Leu Phe Asp Lys Val Lys Leu Ser Asp Val Gly Phe Val Glu Ala Tyr 
        915                 920                 925             


Asn Asn Cys Thr Gly Gly Gln Glu Val Arg Asp Leu Leu Cys Val Gln 
    930                 935                 940                 


Ser Phe Asn Gly Ile Lys Val Leu Pro Pro Val Leu Ser Glu Asn Gln 
945                 950                 955                 960 


Val Ser Gly Tyr Thr Ala Gly Ala Thr Ala Ser Ser Met Phe Pro Pro 
                965                 970                 975     


Trp Ser Ala Ala Ala Gly Val Pro Phe Ser Leu Ser Val Gln Tyr Arg 
            980                 985                 990         


Ile Asn Gly Leu Gly Val Thr Met  Asn Val Leu Ser Glu  Asn Gln Lys 
        995                 1000                 1005             


Met Ile  Ala Ser Ala Phe Asn  Asn Ala Ile Gly Ala  Ile Gln Glu 
    1010                 1015                 1020             


Gly Phe  Asp Ala Thr Asn Ser  Ala Leu Ala Lys Ile  Gln Ser Val 
    1025                 1030                 1035             


Val Asn  Ala Asn Ala Glu Ala  Leu Asn Asn Leu Leu  Asn Gln Leu 
    1040                 1045                 1050             


Ser Asn  Arg Phe Gly Ala Ile  Ser Ala Ser Leu Gln  Glu Ile Leu 
    1055                 1060                 1065             


Ser Arg  Leu Asp Ala Leu Glu  Ala Gln Ala Gln Ile  Asp Arg Leu 
    1070                 1075                 1080             


Ile Asn  Gly Arg Leu Thr Ala  Leu Asn Ala Tyr Val  Ser Lys Gln 
    1085                 1090                 1095             


Leu Ser  Asp Met Thr Leu Ile  Lys Val Ser Ala Ala  Gln Ala Ile 
    1100                 1105                 1110             


Glu Lys  Val Asn Glu Cys Val  Lys Ser Gln Ser Pro  Arg Ile Asn 
    1115                 1120                 1125             


Phe Cys  Gly Asn Gly Asn His  Ile Leu Ser Leu Val  Gln Asn Ala 
    1130                 1135                 1140             


Pro Tyr  Gly Leu Tyr Phe Leu  His Phe Ser Tyr Val  Pro Thr Ser 
    1145                 1150                 1155             


Phe Thr  Thr Ala Asn Val Ser  Pro Gly Leu Cys Ile  Ser Gly Asp 
    1160                 1165                 1170             


Arg Gly  Leu Ala Pro Lys Ala  Gly Tyr Phe Val Gln  Asp Asp Gly 
    1175                 1180                 1185             


Glu Trp  Lys Phe Thr Gly Ser  Asn Tyr Tyr Tyr Pro  Glu Pro Ile 
    1190                 1195                 1200             


Thr Asp  Lys Asn Ser Val Val  Met Ser Ser Cys Ala  Val Asn Tyr 
    1205                 1210                 1215             


Thr Lys  Ala Pro Glu Val Phe  Leu Asn Thr Ser Ile  Ser Asn Leu 
    1220                 1225                 1230             


Pro Asp  Phe Lys Glu Glu Leu  Asp Lys Trp Phe Lys  Asn Gln Thr 
    1235                 1240                 1245             


Ser Val  Ala Pro Asp Leu Ser  Leu Asp Phe Glu Lys  Leu Asn Val 
    1250                 1255                 1260             


Thr Phe  Leu Asp Leu Ser Asp  Glu Met Asn Arg Ile  Gln Glu Ala 
    1265                 1270                 1275             


Ile Lys  Lys Leu Asn Glu Ser  Tyr Ile Asn Leu Lys  Glu Ile Gly 
    1280                 1285                 1290             


Thr Tyr  Glu Met Tyr Val Lys  Trp Pro Trp Tyr Val  Trp Leu Leu 
    1295                 1300                 1305             


Ile Gly  Leu Ala Gly Val Ala  Val Cys Val Leu Leu  Phe Phe Ile 
    1310                 1315                 1320             


Cys Cys  Cys Thr Gly Cys Gly  Ser Cys Cys Phe Lys  Lys Cys Gly 
    1325                 1330                 1335             


Asn Cys  Cys Asp Glu Tyr Gly  Gly His Gln Asp Ser  Ile Val Ile 
    1340                 1345                 1350             


His Asn  Ile Ser Ser His Glu  Asp 
    1355                 1360     


<210>  17
<211>  1297
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  17

Met Leu Ser Val Phe Ile Leu Phe Leu Pro Ser Cys Leu Gly Tyr Ile 
1               5                   10                  15      


Gly Asp Phe Arg Cys Ile Asn Leu Val Asn Thr Asp Thr Ser Asn Ala 
            20                  25                  30          


Ser Ala Pro Ser Val Ser Thr Glu Val Val Asp Val Ser Lys Gly Ile 
        35                  40                  45              


Gly Thr Tyr Tyr Val Leu Asp Arg Val Tyr Leu Asn Ala Thr Leu Leu 
    50                  55                  60                  


Leu Thr Gly Tyr Tyr Pro Val Asp Gly Ser Asn Tyr Arg Asn Leu Ala 
65                  70                  75                  80  


Leu Thr Gly Thr Asn Thr Leu Ser Leu Asn Trp Tyr Lys Pro Pro Phe 
                85                  90                  95      


Leu Ser Glu Phe Asn Asp Gly Ile Phe Ala Lys Val Lys Asn Leu Lys 
            100                 105                 110         


Ala Ser Leu Pro Lys Asp Ser Thr Ser Tyr Phe Pro Thr Ile Val Ile 
        115                 120                 125             


Gly Ser Asn Phe Val Thr Thr Ser Tyr Thr Val Val Leu Glu Pro Tyr 
    130                 135                 140                 


Asn Gly Ile Ile Met Ala Ser Ile Cys Gln Tyr Thr Ile Cys Leu Leu 
145                 150                 155                 160 


Pro Tyr Thr Asp Cys Lys Pro Asn Thr Gly Gly Asn Lys Leu Ile Gly 
                165                 170                 175     


Phe Trp His Ile Asp Leu Lys Ser Pro Val Cys Ile Leu Lys Arg Asn 
            180                 185                 190         


Phe Thr Phe Asn Val Asn Ala Asp Trp Leu Tyr Phe His Phe Tyr Gln 
        195                 200                 205             


Gln Gly Gly Thr Phe Tyr Ala Tyr Tyr Ala Asp Ala Gly Ser Ala Thr 
    210                 215                 220                 


Thr Phe Leu Phe Ser Ser Tyr Ile Gly Asp Val Leu Thr Gln Tyr Phe 
225                 230                 235                 240 


Val Leu Pro Phe Val Cys Thr Pro Thr Thr Thr Gly Val Phe Ser Pro 
                245                 250                 255     


Gln Tyr Trp Val Thr Pro Leu Val Lys Arg Gln Tyr Leu Phe Asn Phe 
            260                 265                 270         


Asn Gln Lys Gly Thr Ile Thr Ser Ala Val Asp Cys Ala Ser Ser Tyr 
        275                 280                 285             


Thr Ser Glu Ile Lys Cys Lys Thr Gln Ser Met Asn Pro Asn Thr Gly 
    290                 295                 300                 


Val Tyr Asp Leu Ser Gly Tyr Thr Val Gln Pro Val Gly Leu Val Tyr 
305                 310                 315                 320 


Arg Arg Val Arg Asn Leu Pro Asp Cys Arg Ile Glu Asp Trp Leu Ala 
                325                 330                 335     


Ala Lys Thr Val Pro Ser Pro Leu Asn Trp Glu Arg Lys Thr Phe Gln 
            340                 345                 350         


Asn Cys Asn Phe Asn Leu Ser Ser Leu Leu Arg Leu Val Gln Ala Gly 
        355                 360                 365             


Ser Leu Ser Cys Ser Asn Ile Asp Ala Ala Lys Val Tyr Gly Met Cys 
    370                 375                 380                 


Phe Gly Ser Met Ser Ile Asp Lys Phe Ala Ile Pro Asn Ser Arg Arg 
385                 390                 395                 400 


Val Asp Leu Gln Leu Gly Asn Ser Gly Phe Leu Gln Ser Phe Asn Tyr 
                405                 410                 415     


Lys Ile Asp Thr Arg Ala Thr Ser Cys Gln Leu Tyr Tyr Ser Leu Ala 
            420                 425                 430         


Gln Ser Asn Val Thr Val Asn Asn His Asn Pro Ser Ser Trp Asn Arg 
        435                 440                 445             


Arg Tyr Gly Phe Asn Asp Val Ala Thr Phe Gly Arg Gly Lys His Asp 
    450                 455                 460                 


Val Ala Tyr Ala Glu Ala Cys Phe Thr Val Gly Ala Ser Tyr Cys Pro 
465                 470                 475                 480 


Cys Ala Asn Pro Ser Ile Val Ser Pro Cys Thr Thr Gly Lys Pro Lys 
                485                 490                 495     


Phe Ala Asn Cys Pro Thr Gly Thr Thr Asn Arg Glu Cys Asn Val Leu 
            500                 505                 510         


Ala Leu Gly Ser Asn Leu Phe Lys Cys Asp Cys Thr Cys Asn Pro Ser 
        515                 520                 525             


Pro Leu Thr Thr Tyr Asp Leu Arg Cys Leu Gln Gly Arg Ser Met Leu 
    530                 535                 540                 


Gly Val Gly Asp His Cys Glu Gly Leu Gly Val Leu Glu Asp Lys Cys 
545                 550                 555                 560 


Gly Gly Ser Asn Thr Cys Asn Cys Ser Ala Asp Ala Phe Val Gly Trp 
                565                 570                 575     


Ala Lys Asp Ser Cys Leu Ser Asn Gly Arg Cys His Ile Phe Ser Asn 
            580                 585                 590         


Leu Met Leu Asn Gly Ile Asn Ser Gly Thr Thr Cys Ser Thr Asp Leu 
        595                 600                 605             


Gln Leu Pro Asn Thr Glu Val Val Thr Gly Ile Cys Val Lys Tyr Asp 
    610                 615                 620                 


Leu Tyr Gly Ile Thr Gly Gln Gly Val Phe Lys Glu Val Lys Ala Asp 
625                 630                 635                 640 


Tyr Tyr Asn Ser Trp Gln Asn Leu Leu Tyr Asp Val Asn Gly Asn Leu 
                645                 650                 655     


Asn Gly Phe Arg Asp Ile Val Thr Asn Lys Thr Tyr Leu Thr Arg Ser 
            660                 665                 670         


Cys Tyr Ser Gly Arg Val Ser Ala Ala Tyr His Gln Asp Ala Pro Glu 
        675                 680                 685             


Pro Ala Leu Leu Tyr Arg Asn Leu Lys Cys Asp Tyr Val Phe Asn Asn 
    690                 695                 700                 


Asn Ile Phe Arg Glu Glu Thr Pro Leu Asn Tyr Phe Asp Ser Tyr Leu 
705                 710                 715                 720 


Gly Cys Val Val Asn Ala Asp Asn Ser Thr Glu Gln Ala Val Asp Ala 
                725                 730                 735     


Cys Asp Leu Arg Met Gly Ser Gly Leu Cys Val Asn Tyr Ser Thr Ala 
            740                 745                 750         


His Arg Ala Arg Thr Ser Val Ser Thr Gly Tyr Lys Leu Thr Thr Phe 
        755                 760                 765             


Glu Pro Phe Thr Val Ser Ile Val Asn Asp Ser Val Glu Ser Val Gly 
    770                 775                 780                 


Gly Leu Tyr Glu Met Gln Ile Pro Thr Asn Phe Thr Ile Ala Ser His 
785                 790                 795                 800 


Gln Glu Phe Ile Gln Thr Arg Ala Pro Lys Val Thr Ile Asp Cys Ala 
                805                 810                 815     


Ala Phe Val Cys Gly Asp Tyr Thr Thr Cys Arg Gln Gln Leu Val Glu 
            820                 825                 830         


Tyr Gly Ser Phe Cys Asp Asn Ile Asn Ala Ile Leu Gly Glu Val Asn 
        835                 840                 845             


Asn Leu Ile Asp Thr Met Gln Leu Gln Val Ala Ser Ala Leu Ile Gln 
    850                 855                 860                 


Gly Val Thr Leu Ser Ser Arg Leu Ala Asp Gly Ile Ser Gly Gln Ile 
865                 870                 875                 880 


Asp Asp Ile Asn Phe Ser Pro Leu Leu Gly Cys Leu Gly Ser Gln Cys 
                885                 890                 895     


Ser Glu Gly Thr Met Ala Ala Gln Gly Arg Ser Thr Val Glu Asp Leu 
            900                 905                 910         


Leu Phe Asp Lys Val Lys Leu Ser Asp Val Gly Phe Val Glu Ala Tyr 
        915                 920                 925             


Asn Asn Cys Thr Gly Gly Gln Glu Val Arg Asp Leu Leu Cys Val Gln 
    930                 935                 940                 


Ser Phe Asn Gly Ile Lys Val Leu Pro Pro Val Leu Ser Glu Asn Gln 
945                 950                 955                 960 


Val Ser Gly Tyr Thr Ala Gly Ala Thr Ala Ser Ser Met Phe Pro Pro 
                965                 970                 975     


Trp Ser Ala Ala Ala Gly Val Pro Phe Ser Leu Ser Val Gln Tyr Arg 
            980                 985                 990         


Ile Asn Gly Leu Gly Val Thr Met  Asn Val Leu Ser Glu  Asn Gln Lys 
        995                 1000                 1005             


Met Ile  Ala Ser Ala Phe Asn  Asn Ala Ile Gly Ala  Ile Gln Glu 
    1010                 1015                 1020             


Gly Phe  Asp Ala Thr Asn Ser  Ala Leu Ala Lys Ile  Gln Ser Val 
    1025                 1030                 1035             


Val Asn  Ala Asn Ala Glu Ala  Leu Asn Asn Leu Leu  Asn Gln Leu 
    1040                 1045                 1050             


Ser Asn  Arg Phe Gly Ala Ile  Ser Ala Ser Leu Gln  Glu Ile Leu 
    1055                 1060                 1065             


Ser Arg  Leu Asp Pro Pro Glu  Ala Gln Ala Gln Ile  Asp Arg Leu 
    1070                 1075                 1080             


Ile Asn  Gly Arg Leu Thr Ala  Leu Asn Ala Tyr Val  Ser Lys Gln 
    1085                 1090                 1095             


Leu Ser  Asp Met Thr Leu Ile  Lys Val Ser Ala Ala  Gln Ala Ile 
    1100                 1105                 1110             


Glu Lys  Val Asn Glu Cys Val  Lys Ser Gln Ser Pro  Arg Ile Asn 
    1115                 1120                 1125             


Phe Cys  Gly Asn Gly Asn His  Ile Leu Ser Leu Val  Gln Asn Ala 
    1130                 1135                 1140             


Pro Tyr  Gly Leu Tyr Phe Leu  His Phe Ser Tyr Val  Pro Thr Ser 
    1145                 1150                 1155             


Phe Thr  Thr Ala Asn Val Ser  Pro Gly Leu Cys Ile  Ser Gly Asp 
    1160                 1165                 1170             


Arg Gly  Leu Ala Pro Lys Ala  Gly Tyr Phe Val Gln  Asp Asp Gly 
    1175                 1180                 1185             


Glu Trp  Lys Phe Thr Gly Ser  Asn Tyr Tyr Tyr Pro  Glu Pro Ile 
    1190                 1195                 1200             


Thr Asp  Lys Asn Ser Val Val  Met Ser Ser Cys Ala  Val Asn Tyr 
    1205                 1210                 1215             


Thr Lys  Ala Pro Glu Val Phe  Leu Asn Thr Ser Ile  Ser Asn Leu 
    1220                 1225                 1230             


Pro Asp  Phe Lys Glu Glu Leu  Asp Lys Trp Phe Lys  Asn Gln Thr 
    1235                 1240                 1245             


Ser Val  Ala Pro Asp Leu Ser  Leu Asp Phe Glu Lys  Leu Asn Val 
    1250                 1255                 1260             


Thr Phe  Leu Asp Leu Ser Asp  Glu Met Asn Arg Ile  Gln Glu Ala 
    1265                 1270                 1275             


Ile Lys  Lys Leu Asn Glu Ser  Tyr Ile Asn Leu Lys  Glu Ile Gly 
    1280                 1285                 1290             


Thr Tyr  Glu Met 
    1295         


<210>  18
<211>  1356
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  18

Met Lys Leu Phe Leu Ile Leu Leu Val Leu Pro Leu Ala Ser Cys Phe 
1               5                   10                  15      


Phe Thr Cys Asn Ser Asn Ala Asn Leu Ser Met Leu Gln Leu Gly Val 
            20                  25                  30          


Pro Asp Asn Ser Ser Thr Ile Val Thr Gly Leu Leu Pro Thr His Trp 
        35                  40                  45              


Phe Cys Ala Asn Gln Ser Thr Ser Val Tyr Ser Ala Asn Gly Phe Phe 
    50                  55                  60                  


Tyr Ile Asp Val Gly Asn His Arg Ser Ala Phe Ala Leu His Thr Gly 
65                  70                  75                  80  


Tyr Tyr Asp Ala Asn Gln Tyr Tyr Ile Tyr Val Thr Asn Glu Ile Gly 
                85                  90                  95      


Leu Asn Ala Ser Val Thr Leu Lys Ile Cys Lys Phe Ser Arg Asn Thr 
            100                 105                 110         


Thr Phe Asp Phe Leu Ser Asn Ala Ser Ser Ser Phe Asp Cys Ile Val 
        115                 120                 125             


Asn Leu Leu Phe Thr Glu Gln Leu Gly Ala Pro Leu Gly Ile Thr Ile 
    130                 135                 140                 


Ser Gly Glu Thr Val Arg Leu His Leu Tyr Asn Val Thr Arg Thr Phe 
145                 150                 155                 160 


Tyr Val Pro Ala Ala Tyr Lys Leu Thr Lys Leu Ser Val Lys Cys Tyr 
                165                 170                 175     


Phe Asn Tyr Ser Cys Val Phe Ser Val Val Asn Ala Thr Val Thr Val 
            180                 185                 190         


Asn Val Thr Thr His Asn Gly Arg Val Val Asn Tyr Thr Val Cys Asp 
        195                 200                 205             


Asp Cys Asn Gly Tyr Thr Asp Asn Ile Phe Ser Val Gln Gln Asp Gly 
    210                 215                 220                 


Arg Ile Pro Asn Gly Phe Pro Phe Asn Asn Trp Phe Leu Leu Thr Asn 
225                 230                 235                 240 


Gly Ser Thr Leu Val Asp Gly Val Ser Arg Leu Tyr Gln Pro Leu Arg 
                245                 250                 255     


Leu Thr Cys Leu Trp Pro Val Pro Gly Leu Lys Ser Ser Thr Gly Phe 
            260                 265                 270         


Val Tyr Phe Asn Ala Thr Gly Ser Asp Val Asn Cys Asn Gly Tyr Gln 
        275                 280                 285             


His Asn Ser Val Val Asp Val Met Arg Tyr Asn Leu Asn Phe Ser Ala 
    290                 295                 300                 


Asn Ser Leu Asp Asn Leu Lys Ser Gly Val Ile Val Phe Lys Thr Leu 
305                 310                 315                 320 


Gln Tyr Asp Val Leu Phe Tyr Cys Ser Asn Ser Ser Ser Gly Val Leu 
                325                 330                 335     


Asp Thr Thr Ile Pro Phe Gly Pro Ser Ser Gln Pro Tyr Tyr Cys Phe 
            340                 345                 350         


Ile Asn Ser Thr Ile Asn Thr Thr His Val Ser Thr Phe Val Gly Ile 
        355                 360                 365             


Leu Pro Pro Thr Val Arg Glu Ile Val Val Ala Arg Thr Gly Gln Phe 
    370                 375                 380                 


Tyr Ile Asn Gly Phe Lys Tyr Phe Asp Leu Gly Phe Ile Glu Ala Val 
385                 390                 395                 400 


Asn Phe Asn Val Thr Thr Ala Ser Ala Thr Asp Phe Trp Thr Val Ala 
                405                 410                 415     


Phe Ala Thr Phe Val Asp Val Leu Val Asn Val Ser Ala Thr Asn Ile 
            420                 425                 430         


Gln Asn Leu Leu Tyr Cys Asp Ser Pro Phe Glu Lys Leu Gln Cys Glu 
        435                 440                 445             


His Leu Gln Phe Gly Leu Gln Asp Gly Phe Tyr Ser Ala Asn Phe Leu 
    450                 455                 460                 


Asp Asp Asn Val Leu Pro Glu Thr Tyr Val Ala Leu Pro Ile Tyr Tyr 
465                 470                 475                 480 


Gln His Thr Asp Ile Asn Phe Thr Ala Thr Ala Ser Phe Gly Gly Ser 
                485                 490                 495     


Cys Tyr Val Cys Lys Pro His Gln Val Asn Ile Ser Leu Asn Gly Asn 
            500                 505                 510         


Thr Ser Val Cys Val Arg Thr Ser His Phe Ser Ile Arg Tyr Ile Tyr 
        515                 520                 525             


Asn Arg Val Lys Ser Gly Ser Pro Gly Asp Ser Ser Trp His Ile Tyr 
    530                 535                 540                 


Leu Lys Ser Gly Thr Cys Pro Phe Ser Phe Ser Lys Leu Asn Asn Phe 
545                 550                 555                 560 


Gln Lys Phe Lys Thr Ile Cys Phe Ser Thr Val Glu Val Pro Gly Ser 
                565                 570                 575     


Cys Asn Phe Pro Leu Glu Ala Thr Trp His Tyr Thr Ser Tyr Thr Ile 
            580                 585                 590         


Val Gly Ala Leu Tyr Val Thr Trp Ser Glu Gly Asn Ser Ile Thr Gly 
        595                 600                 605             


Val Pro Tyr Pro Val Ser Gly Ile Arg Glu Phe Ser Asn Leu Val Leu 
    610                 615                 620                 


Asn Asn Cys Thr Lys Tyr Asn Ile Tyr Asp Tyr Val Gly Thr Gly Ile 
625                 630                 635                 640 


Ile Arg Ser Ser Asn Gln Ser Leu Ala Gly Gly Ile Thr Tyr Val Ser 
                645                 650                 655     


Asn Ser Gly Asn Leu Leu Gly Phe Lys Asn Val Ser Thr Gly Asn Ile 
            660                 665                 670         


Phe Ile Val Thr Pro Cys Asn Gln Pro Asp Gln Val Ala Val Tyr Gln 
        675                 680                 685             


Gln Ser Ile Ile Gly Ala Met Thr Ala Val Asn Glu Ser Arg Tyr Gly 
    690                 695                 700                 


Leu Gln Asn Leu Leu Gln Leu Pro Asn Phe Tyr Tyr Val Ser Asn Gly 
705                 710                 715                 720 


Gly Asn Asn Cys Thr Thr Ala Val Met Thr Tyr Ser Asn Phe Gly Ile 
                725                 730                 735     


Cys Ala Asp Gly Ser Leu Ile Pro Val Arg Pro Arg Asn Ser Ser Asp 
            740                 745                 750         


Asn Gly Ile Ser Ala Ile Ile Thr Ala Asn Leu Ser Ile Pro Ser Asn 
        755                 760                 765             


Trp Thr Thr Ser Val Gln Val Glu Tyr Leu Gln Ile Thr Ser Thr Pro 
    770                 775                 780                 


Ile Val Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn Pro Arg Cys 
785                 790                 795                 800 


Lys Asn Leu Leu Lys Gln Tyr Thr Ser Ala Cys Lys Thr Ile Glu Asp 
                805                 810                 815     


Ala Leu Arg Leu Ser Ala His Leu Glu Thr Asn Asp Val Ser Ser Met 
            820                 825                 830         


Leu Thr Phe Asp Ser Asn Ala Phe Ser Leu Ala Asn Val Thr Ser Phe 
        835                 840                 845             


Gly Asp Tyr Asn Leu Ser Ser Val Leu Pro Gln Arg Asn Ile Arg Ser 
    850                 855                 860                 


Ser Arg Ile Ala Gly Arg Ser Ala Leu Glu Asp Leu Leu Phe Ser Lys 
865                 870                 875                 880 


Val Val Thr Ser Gly Leu Gly Thr Val Asp Val Asp Tyr Lys Ser Cys 
                885                 890                 895     


Thr Lys Gly Leu Ser Ile Ala Asp Leu Ala Cys Ala Gln Tyr Tyr Asn 
            900                 905                 910         


Gly Ile Met Val Leu Pro Gly Val Ala Asp Ala Glu Arg Met Ala Met 
        915                 920                 925             


Tyr Thr Gly Ser Leu Ile Gly Gly Met Val Leu Gly Gly Leu Thr Ser 
    930                 935                 940                 


Ala Ala Ala Ile Pro Phe Ser Leu Ala Leu Gln Ala Arg Leu Asn Tyr 
945                 950                 955                 960 


Val Ala Leu Gln Thr Asp Val Leu Gln Glu Asn Gln Lys Ile Leu Ala 
                965                 970                 975     


Ala Ser Phe Asn Lys Ala Ile Asn Asn Ile Val Ala Ser Phe Ser Ser 
            980                 985                 990         


Val Asn Asp Ala Ile Thr Gln Thr  Ala Glu Ala Ile His  Thr Val Thr 
        995                 1000                 1005             


Ile Ala  Leu Asn Lys Ile Gln  Asp Val Val Asn Gln  Gln Gly Ser 
    1010                 1015                 1020             


Ala Leu  Asn His Leu Thr Ser  Gln Leu Arg His Asn  Phe Gln Ala 
    1025                 1030                 1035             


Ile Ser  Asn Ser Ile Gln Ala  Ile Tyr Asp Arg Leu  Asp Ser Ile 
    1040                 1045                 1050             


Gln Ala  Asp Gln Gln Val Asp  Arg Leu Ile Thr Gly  Arg Leu Ala 
    1055                 1060                 1065             


Ala Leu  Asn Ala Phe Val Ser  Gln Val Leu Asn Lys  Tyr Thr Glu 
    1070                 1075                 1080             


Val Arg  Gly Ser Arg Arg Leu  Ala Gln Gln Lys Ile  Asn Glu Cys 
    1085                 1090                 1095             


Val Lys  Ser Gln Ser Asn Arg  Tyr Gly Phe Cys Gly  Asn Gly Thr 
    1100                 1105                 1110             


His Ile  Phe Ser Ile Val Asn  Ser Ala Pro Asp Gly  Leu Leu Phe 
    1115                 1120                 1125             


Leu His  Thr Val Leu Leu Pro  Thr Asp Tyr Lys Asn  Val Lys Ala 
    1130                 1135                 1140             


Trp Ser  Gly Ile Cys Val Asp  Gly Ile Tyr Gly Tyr  Val Leu Arg 
    1145                 1150                 1155             


Gln Pro  Asn Leu Val Leu Tyr  Ser Asp Asn Gly Val  Phe Arg Val 
    1160                 1165                 1170             


Thr Ser  Arg Ile Met Phe Gln  Pro Arg Leu Pro Val  Leu Ser Asp 
    1175                 1180                 1185             


Phe Val  Gln Ile Tyr Asn Cys  Asn Val Thr Phe Val  Asn Ile Ser 
    1190                 1195                 1200             


Arg Val  Glu Leu His Thr Val  Ile Pro Asp Tyr Val  Asp Val Asn 
    1205                 1210                 1215             


Lys Thr  Leu Gln Glu Phe Ala  Gln Asn Leu Pro Lys  Tyr Val Lys 
    1220                 1225                 1230             


Pro Asn  Phe Asp Leu Thr Pro  Phe Asn Leu Thr Tyr  Leu Asn Leu 
    1235                 1240                 1245             


Ser Ser  Glu Leu Lys Gln Leu  Glu Ala Lys Thr Ala  Ser Leu Phe 
    1250                 1255                 1260             


Gln Thr  Thr Val Glu Leu Gln  Gly Leu Ile Asp Gln  Ile Asn Ser 
    1265                 1270                 1275             


Thr Tyr  Val Asp Leu Lys Leu  Leu Asn Arg Phe Glu  Asn Tyr Ile 
    1280                 1285                 1290             


Lys Trp  Pro Trp Trp Val Trp  Leu Ile Ile Ser Val  Val Phe Val 
    1295                 1300                 1305             


Val Leu  Leu Ser Leu Leu Val  Phe Cys Cys Leu Ser  Thr Gly Cys 
    1310                 1315                 1320             


Cys Gly  Cys Cys Asn Cys Leu  Thr Ser Ser Met Arg  Gly Cys Cys 
    1325                 1330                 1335             


Asp Cys  Gly Ser Thr Lys Leu  Pro Tyr Tyr Glu Phe  Glu Lys Val 
    1340                 1345                 1350             


His Val  Gln 
    1355     


<210>  19
<211>  1291
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  19

Met Lys Leu Phe Leu Ile Leu Leu Val Leu Pro Leu Ala Ser Cys Phe 
1               5                   10                  15      


Phe Thr Cys Asn Ser Asn Ala Asn Leu Ser Met Leu Gln Leu Gly Val 
            20                  25                  30          


Pro Asp Asn Ser Ser Thr Ile Val Thr Gly Leu Leu Pro Thr His Trp 
        35                  40                  45              


Phe Cys Ala Asn Gln Ser Thr Ser Val Tyr Ser Ala Asn Gly Phe Phe 
    50                  55                  60                  


Tyr Ile Asp Val Gly Asn His Arg Ser Ala Phe Ala Leu His Thr Gly 
65                  70                  75                  80  


Tyr Tyr Asp Ala Asn Gln Tyr Tyr Ile Tyr Val Thr Asn Glu Ile Gly 
                85                  90                  95      


Leu Asn Ala Ser Val Thr Leu Lys Ile Cys Lys Phe Ser Arg Asn Thr 
            100                 105                 110         


Thr Phe Asp Phe Leu Ser Asn Ala Ser Ser Ser Phe Asp Cys Ile Val 
        115                 120                 125             


Asn Leu Leu Phe Thr Glu Gln Leu Gly Ala Pro Leu Gly Ile Thr Ile 
    130                 135                 140                 


Ser Gly Glu Thr Val Arg Leu His Leu Tyr Asn Val Thr Arg Thr Phe 
145                 150                 155                 160 


Tyr Val Pro Ala Ala Tyr Lys Leu Thr Lys Leu Ser Val Lys Cys Tyr 
                165                 170                 175     


Phe Asn Tyr Ser Cys Val Phe Ser Val Val Asn Ala Thr Val Thr Val 
            180                 185                 190         


Asn Val Thr Thr His Asn Gly Arg Val Val Asn Tyr Thr Val Cys Asp 
        195                 200                 205             


Asp Cys Asn Gly Tyr Thr Asp Asn Ile Phe Ser Val Gln Gln Asp Gly 
    210                 215                 220                 


Arg Ile Pro Asn Gly Phe Pro Phe Asn Asn Trp Phe Leu Leu Thr Asn 
225                 230                 235                 240 


Gly Ser Thr Leu Val Asp Gly Val Ser Arg Leu Tyr Gln Pro Leu Arg 
                245                 250                 255     


Leu Thr Cys Leu Trp Pro Val Pro Gly Leu Lys Ser Ser Thr Gly Phe 
            260                 265                 270         


Val Tyr Phe Asn Ala Thr Gly Ser Asp Val Asn Cys Asn Gly Tyr Gln 
        275                 280                 285             


His Asn Ser Val Val Asp Val Met Arg Tyr Asn Leu Asn Phe Ser Ala 
    290                 295                 300                 


Asn Ser Leu Asp Asn Leu Lys Ser Gly Val Ile Val Phe Lys Thr Leu 
305                 310                 315                 320 


Gln Tyr Asp Val Leu Phe Tyr Cys Ser Asn Ser Ser Ser Gly Val Leu 
                325                 330                 335     


Asp Thr Thr Ile Pro Phe Gly Pro Ser Ser Gln Pro Tyr Tyr Cys Phe 
            340                 345                 350         


Ile Asn Ser Thr Ile Asn Thr Thr His Val Ser Thr Phe Val Gly Ile 
        355                 360                 365             


Leu Pro Pro Thr Val Arg Glu Ile Val Val Ala Arg Thr Gly Gln Phe 
    370                 375                 380                 


Tyr Ile Asn Gly Phe Lys Tyr Phe Asp Leu Gly Phe Ile Glu Ala Val 
385                 390                 395                 400 


Asn Phe Asn Val Thr Thr Ala Ser Ala Thr Asp Phe Trp Thr Val Ala 
                405                 410                 415     


Phe Ala Thr Phe Val Asp Val Leu Val Asn Val Ser Ala Thr Asn Ile 
            420                 425                 430         


Gln Asn Leu Leu Tyr Cys Asp Ser Pro Phe Glu Lys Leu Gln Cys Glu 
        435                 440                 445             


His Leu Gln Phe Gly Leu Gln Asp Gly Phe Tyr Ser Ala Asn Phe Leu 
    450                 455                 460                 


Asp Asp Asn Val Leu Pro Glu Thr Tyr Val Ala Leu Pro Ile Tyr Tyr 
465                 470                 475                 480 


Gln His Thr Asp Ile Asn Phe Thr Ala Thr Ala Ser Phe Gly Gly Ser 
                485                 490                 495     


Cys Tyr Val Cys Lys Pro His Gln Val Asn Ile Ser Leu Asn Gly Asn 
            500                 505                 510         


Thr Ser Val Cys Val Arg Thr Ser His Phe Ser Ile Arg Tyr Ile Tyr 
        515                 520                 525             


Asn Arg Val Lys Ser Gly Ser Pro Gly Asp Ser Ser Trp His Ile Tyr 
    530                 535                 540                 


Leu Lys Ser Gly Thr Cys Pro Phe Ser Phe Ser Lys Leu Asn Asn Phe 
545                 550                 555                 560 


Gln Lys Phe Lys Thr Ile Cys Phe Ser Thr Val Glu Val Pro Gly Ser 
                565                 570                 575     


Cys Asn Phe Pro Leu Glu Ala Thr Trp His Tyr Thr Ser Tyr Thr Ile 
            580                 585                 590         


Val Gly Ala Leu Tyr Val Thr Trp Ser Glu Gly Asn Ser Ile Thr Gly 
        595                 600                 605             


Val Pro Tyr Pro Val Ser Gly Ile Arg Glu Phe Ser Asn Leu Val Leu 
    610                 615                 620                 


Asn Asn Cys Thr Lys Tyr Asn Ile Tyr Asp Tyr Val Gly Thr Gly Ile 
625                 630                 635                 640 


Ile Arg Ser Ser Asn Gln Ser Leu Ala Gly Gly Ile Thr Tyr Val Ser 
                645                 650                 655     


Asn Ser Gly Asn Leu Leu Gly Phe Lys Asn Val Ser Thr Gly Asn Ile 
            660                 665                 670         


Phe Ile Val Thr Pro Cys Asn Gln Pro Asp Gln Val Ala Val Tyr Gln 
        675                 680                 685             


Gln Ser Ile Ile Gly Ala Met Thr Ala Val Asn Glu Ser Arg Tyr Gly 
    690                 695                 700                 


Leu Gln Asn Leu Leu Gln Leu Pro Asn Phe Tyr Tyr Val Ser Asn Gly 
705                 710                 715                 720 


Gly Asn Asn Cys Thr Thr Ala Val Met Thr Tyr Ser Asn Phe Gly Ile 
                725                 730                 735     


Cys Ala Asp Gly Ser Leu Ile Pro Val Arg Pro Arg Asn Ser Ser Asp 
            740                 745                 750         


Asn Gly Ile Ser Ala Ile Ile Thr Ala Asn Leu Ser Ile Pro Ser Asn 
        755                 760                 765             


Trp Thr Thr Ser Val Gln Val Glu Tyr Leu Gln Ile Thr Ser Thr Pro 
    770                 775                 780                 


Ile Val Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn Pro Arg Cys 
785                 790                 795                 800 


Lys Asn Leu Leu Lys Gln Tyr Thr Ser Ala Cys Lys Thr Ile Glu Asp 
                805                 810                 815     


Ala Leu Arg Leu Ser Ala His Leu Glu Thr Asn Asp Val Ser Ser Met 
            820                 825                 830         


Leu Thr Phe Asp Ser Asn Ala Phe Ser Leu Ala Asn Val Thr Ser Phe 
        835                 840                 845             


Gly Asp Tyr Asn Leu Ser Ser Val Leu Pro Gln Arg Asn Ile Arg Ser 
    850                 855                 860                 


Ser Arg Ile Ala Gly Arg Ser Ala Leu Glu Asp Leu Leu Phe Ser Lys 
865                 870                 875                 880 


Val Val Thr Ser Gly Leu Gly Thr Val Asp Val Asp Tyr Lys Ser Cys 
                885                 890                 895     


Thr Lys Gly Leu Ser Ile Ala Asp Leu Ala Cys Ala Gln Tyr Tyr Asn 
            900                 905                 910         


Gly Ile Met Val Leu Pro Gly Val Ala Asp Ala Glu Arg Met Ala Met 
        915                 920                 925             


Tyr Thr Gly Ser Leu Ile Gly Gly Met Val Leu Gly Gly Leu Thr Ser 
    930                 935                 940                 


Ala Ala Ala Ile Pro Phe Ser Leu Ala Leu Gln Ala Arg Leu Asn Tyr 
945                 950                 955                 960 


Val Ala Leu Gln Thr Asp Val Leu Gln Glu Asn Gln Lys Ile Leu Ala 
                965                 970                 975     


Ala Ser Phe Asn Lys Ala Ile Asn Asn Ile Val Ala Ser Phe Ser Ser 
            980                 985                 990         


Val Asn Asp Ala Ile Thr Gln Thr  Ala Glu Ala Ile His  Thr Val Thr 
        995                 1000                 1005             


Ile Ala  Leu Asn Lys Ile Gln  Asp Val Val Asn Gln  Gln Gly Ser 
    1010                 1015                 1020             


Ala Leu  Asn His Leu Thr Ser  Gln Leu Arg His Asn  Phe Gln Ala 
    1025                 1030                 1035             


Ile Ser  Asn Ser Ile Gln Ala  Ile Tyr Asp Arg Leu  Asp Pro Pro 
    1040                 1045                 1050             


Gln Ala  Asp Gln Gln Val Asp  Arg Leu Ile Thr Gly  Arg Leu Ala 
    1055                 1060                 1065             


Ala Leu  Asn Ala Phe Val Ser  Gln Val Leu Asn Lys  Tyr Thr Glu 
    1070                 1075                 1080             


Val Arg  Gly Ser Arg Arg Leu  Ala Gln Gln Lys Ile  Asn Glu Cys 
    1085                 1090                 1095             


Val Lys  Ser Gln Ser Asn Arg  Tyr Gly Phe Cys Gly  Asn Gly Thr 
    1100                 1105                 1110             


His Ile  Phe Ser Ile Val Asn  Ser Ala Pro Asp Gly  Leu Leu Phe 
    1115                 1120                 1125             


Leu His  Thr Val Leu Leu Pro  Thr Asp Tyr Lys Asn  Val Lys Ala 
    1130                 1135                 1140             


Trp Ser  Gly Ile Cys Val Asp  Gly Ile Tyr Gly Tyr  Val Leu Arg 
    1145                 1150                 1155             


Gln Pro  Asn Leu Val Leu Tyr  Ser Asp Asn Gly Val  Phe Arg Val 
    1160                 1165                 1170             


Thr Ser  Arg Ile Met Phe Gln  Pro Arg Leu Pro Val  Leu Ser Asp 
    1175                 1180                 1185             


Phe Val  Gln Ile Tyr Asn Cys  Asn Val Thr Phe Val  Asn Ile Ser 
    1190                 1195                 1200             


Arg Val  Glu Leu His Thr Val  Ile Pro Asp Tyr Val  Asp Val Asn 
    1205                 1210                 1215             


Lys Thr  Leu Gln Glu Phe Ala  Gln Asn Leu Pro Lys  Tyr Val Lys 
    1220                 1225                 1230             


Pro Asn  Phe Asp Leu Thr Pro  Phe Asn Leu Thr Tyr  Leu Asn Leu 
    1235                 1240                 1245             


Ser Ser  Glu Leu Lys Gln Leu  Glu Ala Lys Thr Ala  Ser Leu Phe 
    1250                 1255                 1260             


Gln Thr  Thr Val Glu Leu Gln  Gly Leu Ile Asp Gln  Ile Asn Ser 
    1265                 1270                 1275             


Thr Tyr  Val Asp Leu Lys Leu  Leu Asn Arg Phe Glu  Asn 
    1280                 1285                 1290     


<210>  20
<211>  1171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  20

Met Phe Val Leu Leu Val Ala Tyr Ala Leu Leu His Ile Ala Gly Cys 
1               5                   10                  15      


Gln Thr Thr Asn Gly Thr Asn Thr Ser His Ser Val Cys Asn Gly Cys 
            20                  25                  30          


Val Gly His Ser Glu Asn Val Phe Ala Val Glu Ser Gly Gly Tyr Ile 
        35                  40                  45              


Pro Ser Asn Phe Ala Phe Asn Asn Trp Phe Leu Leu Thr Asn Thr Ser 
    50                  55                  60                  


Ser Val Val Asp Gly Val Val Arg Ser Phe Gln Pro Leu Leu Leu Asn 
65                  70                  75                  80  


Cys Leu Trp Ser Val Ser Gly Ser Gln Phe Thr Thr Gly Phe Val Tyr 
                85                  90                  95      


Phe Asn Gly Thr Gly Arg Gly Ala Cys Lys Gly Phe Tyr Ser Asn Ala 
            100                 105                 110         


Ser Ser Asp Val Ile Arg Tyr Asn Ile Asn Phe Glu Glu Asn Leu Arg 
        115                 120                 125             


Arg Gly Thr Ile Leu Phe Lys Thr Ser Tyr Gly Ala Val Val Phe Tyr 
    130                 135                 140                 


Cys Thr Asn Asn Thr Leu Val Ser Gly Asp Ala His Ile Pro Ser Gly 
145                 150                 155                 160 


Thr Val Leu Gly Asn Phe Tyr Cys Phe Val Asn Thr Thr Ile Gly Asn 
                165                 170                 175     


Glu Thr Thr Ser Ala Phe Val Gly Ala Leu Pro Lys Thr Val Arg Glu 
            180                 185                 190         


Phe Val Ile Ser Arg Thr Gly His Phe Tyr Ile Asn Gly Tyr Arg Tyr 
        195                 200                 205             


Phe Ser Leu Gly Asp Val Glu Ala Val Asn Phe Asn Val Thr Asn Ala 
    210                 215                 220                 


Ala Thr Thr Val Cys Thr Val Ala Leu Ala Ser Tyr Ala Asp Val Leu 
225                 230                 235                 240 


Val Asn Val Ser Gln Thr Ala Ile Ala Asn Ile Ile Tyr Cys Asn Ser 
                245                 250                 255     


Val Ile Asn Arg Leu Arg Cys Asp Gln Leu Ser Phe Asp Val Pro Asp 
            260                 265                 270         


Gly Phe Tyr Ser Thr Ser Pro Ile Gln Pro Val Glu Leu Pro Val Ser 
        275                 280                 285             


Ile Val Ser Leu Pro Val Tyr His Lys His Thr Phe Ile Val Leu Tyr 
    290                 295                 300                 


Val Asn Phe Glu His Arg Arg Gly Pro Gly Lys Cys Tyr Asn Cys Arg 
305                 310                 315                 320 


Pro Ala Val Ile Asn Ile Thr Leu Ala Asn Phe Asn Glu Thr Lys Gly 
                325                 330                 335     


Pro Leu Cys Val Asp Thr Ser His Phe Thr Thr Gln Phe Val Asp Asn 
            340                 345                 350         


Val Lys Leu Ala Arg Trp Ser Ala Ser Ile Asn Thr Gly Asn Cys Pro 
        355                 360                 365             


Phe Ser Phe Gly Lys Val Asn Asn Phe Val Lys Phe Gly Ser Val Cys 
    370                 375                 380                 


Phe Ser Leu Lys Asp Ile Pro Gly Gly Cys Ala Met Pro Ile Met Ala 
385                 390                 395                 400 


Asn Leu Val Asn Ser Lys Ser His Asn Ile Gly Ser Leu Tyr Val Ser 
                405                 410                 415     


Trp Ser Asp Gly Asp Val Ile Thr Gly Val Pro Lys Pro Val Glu Gly 
            420                 425                 430         


Val Ser Ser Phe Met Asn Val Thr Leu Asn Lys Cys Thr Lys Tyr Asn 
        435                 440                 445             


Ile Tyr Asp Val Ser Gly Val Gly Val Ile Arg Ile Ser Asn Asp Thr 
    450                 455                 460                 


Phe Leu Asn Gly Ile Thr Tyr Thr Ser Thr Ser Gly Asn Leu Leu Gly 
465                 470                 475                 480 


Phe Lys Asp Val Thr Asn Gly Thr Ile Tyr Ser Ile Thr Pro Cys Asn 
                485                 490                 495     


Pro Pro Asp Gln Leu Val Val Tyr Gln Gln Ala Val Val Gly Ala Met 
            500                 505                 510         


Leu Ser Glu Asn Phe Thr Ser Tyr Gly Phe Ser Asn Val Val Glu Met 
        515                 520                 525             


Pro Lys Phe Phe Tyr Ala Ser Asn Gly Thr Tyr Asn Cys Thr Asp Ala 
    530                 535                 540                 


Val Leu Thr Tyr Ser Ser Phe Gly Val Cys Ala Asp Gly Ser Ile Ile 
545                 550                 555                 560 


Ala Val Gln Pro Arg Asn Val Ser Tyr Asp Ser Val Ser Ala Ile Val 
                565                 570                 575     


Thr Ala Asn Leu Ser Ile Pro Phe Asn Trp Thr Thr Ser Val Gln Val 
            580                 585                 590         


Glu Tyr Leu Gln Ile Thr Ser Thr Pro Ile Val Val Asp Cys Ser Thr 
        595                 600                 605             


Tyr Val Cys Asn Gly Asn Val Arg Cys Val Glu Leu Leu Lys Gln Tyr 
    610                 615                 620                 


Thr Ser Ala Cys Lys Thr Ile Glu Asp Ala Leu Arg Asn Ser Ala Met 
625                 630                 635                 640 


Leu Glu Ser Ala Asp Val Ser Glu Met Leu Thr Phe Asp Lys Lys Ala 
                645                 650                 655     


Phe Thr Leu Ala Asn Val Ser Ser Phe Gly Asp Tyr Asn Leu Ser Ser 
            660                 665                 670         


Val Ile Pro Ser Leu Pro Arg Ser Gly Ser Arg Val Ala Gly Arg Ser 
        675                 680                 685             


Ala Ile Glu Asp Ile Leu Phe Ser Lys Leu Val Thr Ser Gly Leu Gly 
    690                 695                 700                 


Thr Val Asp Ala Asp Tyr Lys Lys Cys Thr Lys Gly Leu Ser Ile Ala 
705                 710                 715                 720 


Asp Leu Ala Cys Ala Gln Tyr Tyr Asn Gly Ile Met Val Leu Pro Gly 
                725                 730                 735     


Val Ala Asp Ala Glu Arg Met Ala Met Tyr Thr Gly Ser Leu Ile Gly 
            740                 745                 750         


Gly Ile Ala Leu Gly Gly Leu Thr Ser Ala Ala Ser Ile Pro Phe Ser 
        755                 760                 765             


Leu Ala Ile Gln Ser Arg Leu Asn Tyr Val Ala Leu Gln Thr Asp Val 
    770                 775                 780                 


Leu Gln Glu Asn Gln Arg Ile Leu Ala Ala Ser Phe Asn Lys Ala Met 
785                 790                 795                 800 


Thr Asn Ile Val Asp Ala Phe Thr Gly Val Asn Asp Ala Ile Thr Gln 
                805                 810                 815     


Thr Ser Gln Ala Leu Gln Thr Val Ala Thr Ala Leu Asn Lys Ile Gln 
            820                 825                 830         


Asp Val Val Asn Gln Gln Gly Asn Ser Leu Asn His Leu Thr Ser Gln 
        835                 840                 845             


Leu Arg Gln Asn Phe Gln Ala Ile Ser Ser Ser Ile Gln Ala Ile Tyr 
    850                 855                 860                 


Asp Arg Leu Asp Ile Ile Gln Ala Asp Gln Gln Val Asp Arg Leu Ile 
865                 870                 875                 880 


Thr Gly Arg Leu Ala Ala Leu Asn Val Phe Val Ser His Thr Leu Thr 
                885                 890                 895     


Lys Tyr Thr Glu Val Arg Ala Ser Arg Gln Leu Ala Gln Gln Lys Val 
            900                 905                 910         


Asn Glu Cys Val Lys Ser Gln Ser Lys Arg Tyr Gly Phe Cys Gly Asn 
        915                 920                 925             


Gly Thr His Ile Phe Ser Leu Val Asn Ala Ala Pro Glu Gly Leu Val 
    930                 935                 940                 


Phe Leu His Thr Val Leu Leu Pro Thr Gln Tyr Lys Asp Val Glu Ala 
945                 950                 955                 960 


Trp Ser Gly Leu Cys Val Asp Gly Ile Asn Gly Tyr Val Leu Arg Gln 
                965                 970                 975     


Pro Asn Leu Ala Leu Tyr Lys Glu Gly Asn Tyr Tyr Arg Ile Thr Ser 
            980                 985                 990         


Arg Ile Met Phe Glu Pro Arg Ile  Pro Thr Ile Ala Asp  Phe Val Gln 
        995                 1000                 1005             


Ile Glu  Asn Cys Asn Val Thr  Phe Val Asn Ile Ser  Arg Ser Glu 
    1010                 1015                 1020             


Leu Gln  Thr Ile Val Pro Glu  Tyr Ile Asp Val Asn  Lys Thr Leu 
    1025                 1030                 1035             


Gln Glu  Leu Ser Tyr Lys Leu  Pro Asn Tyr Thr Val  Pro Asp Leu 
    1040                 1045                 1050             


Val Val  Glu Gln Tyr Asn Gln  Thr Ile Leu Asn Leu  Thr Ser Glu 
    1055                 1060                 1065             


Ile Ser  Thr Leu Glu Asn Lys  Ser Ala Glu Leu Asn  Tyr Thr Val 
    1070                 1075                 1080             


Gln Lys  Leu Gln Thr Leu Ile  Asp Asn Ile Asn Ser  Thr Leu Val 
    1085                 1090                 1095             


Asp Leu  Lys Trp Leu Asn Arg  Val Glu Thr Tyr Ile  Lys Trp Pro 
    1100                 1105                 1110             


Trp Trp  Val Trp Leu Cys Ile  Ser Val Val Leu Ile  Phe Val Val 
    1115                 1120                 1125             


Ser Met  Leu Leu Leu Cys Cys  Cys Ser Thr Gly Cys  Cys Gly Phe 
    1130                 1135                 1140             


Phe Ser  Cys Phe Ala Ser Ser  Ile Arg Gly Cys Cys  Glu Ser Thr 
    1145                 1150                 1155             


Lys Leu  Pro Tyr Tyr Asp Val  Glu Lys Ile His Ile  Gln 
    1160                 1165                 1170     


<210>  21
<211>  1108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  21

Met Phe Val Leu Leu Val Ala Tyr Ala Leu Leu His Ile Ala Gly Cys 
1               5                   10                  15      


Gln Thr Thr Asn Gly Thr Asn Thr Ser His Ser Val Cys Asn Gly Cys 
            20                  25                  30          


Val Gly His Ser Glu Asn Val Phe Ala Val Glu Ser Gly Gly Tyr Ile 
        35                  40                  45              


Pro Ser Asn Phe Ala Phe Asn Asn Trp Phe Leu Leu Thr Asn Thr Ser 
    50                  55                  60                  


Ser Val Val Asp Gly Val Val Arg Ser Phe Gln Pro Leu Leu Leu Asn 
65                  70                  75                  80  


Cys Leu Trp Ser Val Ser Gly Ser Gln Phe Thr Thr Gly Phe Val Tyr 
                85                  90                  95      


Phe Asn Gly Thr Gly Arg Gly Ala Cys Lys Gly Phe Tyr Ser Asn Ala 
            100                 105                 110         


Ser Ser Asp Val Ile Arg Tyr Asn Ile Asn Phe Glu Glu Asn Leu Arg 
        115                 120                 125             


Arg Gly Thr Ile Leu Phe Lys Thr Ser Tyr Gly Ala Val Val Phe Tyr 
    130                 135                 140                 


Cys Thr Asn Asn Thr Leu Val Ser Gly Asp Ala His Ile Pro Ser Gly 
145                 150                 155                 160 


Thr Val Leu Gly Asn Phe Tyr Cys Phe Val Asn Thr Thr Ile Gly Asn 
                165                 170                 175     


Glu Thr Thr Ser Ala Phe Val Gly Ala Leu Pro Lys Thr Val Arg Glu 
            180                 185                 190         


Phe Val Ile Ser Arg Thr Gly His Phe Tyr Ile Asn Gly Tyr Arg Tyr 
        195                 200                 205             


Phe Ser Leu Gly Asp Val Glu Ala Val Asn Phe Asn Val Thr Asn Ala 
    210                 215                 220                 


Ala Thr Thr Val Cys Thr Val Ala Leu Ala Ser Tyr Ala Asp Val Leu 
225                 230                 235                 240 


Val Asn Val Ser Gln Thr Ala Ile Ala Asn Ile Ile Tyr Cys Asn Ser 
                245                 250                 255     


Val Ile Asn Arg Leu Arg Cys Asp Gln Leu Ser Phe Asp Val Pro Asp 
            260                 265                 270         


Gly Phe Tyr Ser Thr Ser Pro Ile Gln Pro Val Glu Leu Pro Val Ser 
        275                 280                 285             


Ile Val Ser Leu Pro Val Tyr His Lys His Thr Phe Ile Val Leu Tyr 
    290                 295                 300                 


Val Asn Phe Glu His Arg Arg Gly Pro Gly Lys Cys Tyr Asn Cys Arg 
305                 310                 315                 320 


Pro Ala Val Ile Asn Ile Thr Leu Ala Asn Phe Asn Glu Thr Lys Gly 
                325                 330                 335     


Pro Leu Cys Val Asp Thr Ser His Phe Thr Thr Gln Phe Val Asp Asn 
            340                 345                 350         


Val Lys Leu Ala Arg Trp Ser Ala Ser Ile Asn Thr Gly Asn Cys Pro 
        355                 360                 365             


Phe Ser Phe Gly Lys Val Asn Asn Phe Val Lys Phe Gly Ser Val Cys 
    370                 375                 380                 


Phe Ser Leu Lys Asp Ile Pro Gly Gly Cys Ala Met Pro Ile Met Ala 
385                 390                 395                 400 


Asn Leu Val Asn Ser Lys Ser His Asn Ile Gly Ser Leu Tyr Val Ser 
                405                 410                 415     


Trp Ser Asp Gly Asp Val Ile Thr Gly Val Pro Lys Pro Val Glu Gly 
            420                 425                 430         


Val Ser Ser Phe Met Asn Val Thr Leu Asn Lys Cys Thr Lys Tyr Asn 
        435                 440                 445             


Ile Tyr Asp Val Ser Gly Val Gly Val Ile Arg Ile Ser Asn Asp Thr 
    450                 455                 460                 


Phe Leu Asn Gly Ile Thr Tyr Thr Ser Thr Ser Gly Asn Leu Leu Gly 
465                 470                 475                 480 


Phe Lys Asp Val Thr Asn Gly Thr Ile Tyr Ser Ile Thr Pro Cys Asn 
                485                 490                 495     


Pro Pro Asp Gln Leu Val Val Tyr Gln Gln Ala Val Val Gly Ala Met 
            500                 505                 510         


Leu Ser Glu Asn Phe Thr Ser Tyr Gly Phe Ser Asn Val Val Glu Met 
        515                 520                 525             


Pro Lys Phe Phe Tyr Ala Ser Asn Gly Thr Tyr Asn Cys Thr Asp Ala 
    530                 535                 540                 


Val Leu Thr Tyr Ser Ser Phe Gly Val Cys Ala Asp Gly Ser Ile Ile 
545                 550                 555                 560 


Ala Val Gln Pro Arg Asn Val Ser Tyr Asp Ser Val Ser Ala Ile Val 
                565                 570                 575     


Thr Ala Asn Leu Ser Ile Pro Phe Asn Trp Thr Thr Ser Val Gln Val 
            580                 585                 590         


Glu Tyr Leu Gln Ile Thr Ser Thr Pro Ile Val Val Asp Cys Ser Thr 
        595                 600                 605             


Tyr Val Cys Asn Gly Asn Val Arg Cys Val Glu Leu Leu Lys Gln Tyr 
    610                 615                 620                 


Thr Ser Ala Cys Lys Thr Ile Glu Asp Ala Leu Arg Asn Ser Ala Met 
625                 630                 635                 640 


Leu Glu Ser Ala Asp Val Ser Glu Met Leu Thr Phe Asp Lys Lys Ala 
                645                 650                 655     


Phe Thr Leu Ala Asn Val Ser Ser Phe Gly Asp Tyr Asn Leu Ser Ser 
            660                 665                 670         


Val Ile Pro Ser Leu Pro Arg Ser Gly Ser Arg Val Ala Gly Arg Ser 
        675                 680                 685             


Ala Ile Glu Asp Ile Leu Phe Ser Lys Leu Val Thr Ser Gly Leu Gly 
    690                 695                 700                 


Thr Val Asp Ala Asp Tyr Lys Lys Cys Thr Lys Gly Leu Ser Ile Ala 
705                 710                 715                 720 


Asp Leu Ala Cys Ala Gln Tyr Tyr Asn Gly Ile Met Val Leu Pro Gly 
                725                 730                 735     


Val Ala Asp Ala Glu Arg Met Ala Met Tyr Thr Gly Ser Leu Ile Gly 
            740                 745                 750         


Gly Ile Ala Leu Gly Gly Leu Thr Ser Ala Ala Ser Ile Pro Phe Ser 
        755                 760                 765             


Leu Ala Ile Gln Ser Arg Leu Asn Tyr Val Ala Leu Gln Thr Asp Val 
    770                 775                 780                 


Leu Gln Glu Asn Gln Arg Ile Leu Ala Ala Ser Phe Asn Lys Ala Met 
785                 790                 795                 800 


Thr Asn Ile Val Asp Ala Phe Thr Gly Val Asn Asp Ala Ile Thr Gln 
                805                 810                 815     


Thr Ser Gln Ala Leu Gln Thr Val Ala Thr Ala Leu Asn Lys Ile Gln 
            820                 825                 830         


Asp Val Val Asn Gln Gln Gly Asn Ser Leu Asn His Leu Thr Ser Gln 
        835                 840                 845             


Leu Arg Gln Asn Phe Gln Ala Ile Ser Ser Ser Ile Gln Ala Ile Tyr 
    850                 855                 860                 


Asp Arg Leu Asp Pro Pro Gln Ala Asp Gln Gln Val Asp Arg Leu Ile 
865                 870                 875                 880 


Thr Gly Arg Leu Ala Ala Leu Asn Val Phe Val Ser His Thr Leu Thr 
                885                 890                 895     


Lys Tyr Thr Glu Val Arg Ala Ser Arg Gln Leu Ala Gln Gln Lys Val 
            900                 905                 910         


Asn Glu Cys Val Lys Ser Gln Ser Lys Arg Tyr Gly Phe Cys Gly Asn 
        915                 920                 925             


Gly Thr His Ile Phe Ser Leu Val Asn Ala Ala Pro Glu Gly Leu Val 
    930                 935                 940                 


Phe Leu His Thr Val Leu Leu Pro Thr Gln Tyr Lys Asp Val Glu Ala 
945                 950                 955                 960 


Trp Ser Gly Leu Cys Val Asp Gly Ile Asn Gly Tyr Val Leu Arg Gln 
                965                 970                 975     


Pro Asn Leu Ala Leu Tyr Lys Glu Gly Asn Tyr Tyr Arg Ile Thr Ser 
            980                 985                 990         


Arg Ile Met Phe Glu Pro Arg Ile  Pro Thr Ile Ala Asp  Phe Val Gln 
        995                 1000                 1005             


Ile Glu  Asn Cys Asn Val Thr  Phe Val Asn Ile Ser  Arg Ser Glu 
    1010                 1015                 1020             


Leu Gln  Thr Ile Val Pro Glu  Tyr Ile Asp Val Asn  Lys Thr Leu 
    1025                 1030                 1035             


Gln Glu  Leu Ser Tyr Lys Leu  Pro Asn Tyr Thr Val  Pro Asp Leu 
    1040                 1045                 1050             


Val Val  Glu Gln Tyr Asn Gln  Thr Ile Leu Asn Leu  Thr Ser Glu 
    1055                 1060                 1065             


Ile Ser  Thr Leu Glu Asn Lys  Ser Ala Glu Leu Asn  Tyr Thr Val 
    1070                 1075                 1080             


Gln Lys  Leu Gln Thr Leu Ile  Asp Asn Ile Asn Ser  Thr Leu Val 
    1085                 1090                 1095             


Asp Leu  Lys Trp Leu Asn Arg  Val Glu Thr 
    1100                 1105             


<210>  22
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GCN4 trimerization domain

<400>  22

Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys 
1               5                   10                  15      


Asp Gly Glu Trp Val Leu Leu Ser Thr Phe 
            20                  25      


<210>  23
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  self-assembling protein nanoparticle sequence

<400>  23

Glu Ser Gln Val Arg Gln Gln Phe Ser Lys Asp Ile Glu Lys Leu Leu 
1               5                   10                  15      


Asn Glu Gln Val Asn Lys Glu Met Gln Ser Ser Asn Leu Tyr Met Ser 
            20                  25                  30          


Met Ser Ser Trp Cys Tyr Thr His Ser Leu Asp Gly Ala Gly Leu Phe 
        35                  40                  45              


Leu Phe Asp His Ala Ala Glu Glu Tyr Glu His Ala Lys Lys Leu Ile 
    50                  55                  60                  


Ile Phe Leu Asn Glu Asn Asn Val Pro Val Gln Leu Thr Ser Ile Ser 
65                  70                  75                  80  


Ala Pro Glu His Lys Phe Glu Gly Leu Thr Gln Ile Phe Gln Lys Ala 
                85                  90                  95      


Tyr Glu His Glu Gln His Ile Ser Glu Ser Ile Asn Asn Ile Val Asp 
            100                 105                 110         


His Ala Ile Lys Ser Lys Asp His Ala Thr Phe Asn Phe Leu Gln Trp 
        115                 120                 125             


Tyr Val Ala Glu Gln His Glu Glu Glu Val Leu Phe Lys Asp Ile Leu 
    130                 135                 140                 


Asp Lys Ile Glu Leu Ile Gly Asn Glu Asn His Gly Leu Tyr Leu Ala 
145                 150                 155                 160 


Asp Gln Tyr Val Lys Gly Ile Ala Lys Ser Arg Lys Ser 
                165                 170             


<210>  24
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  self-assembling protein nanoparticle sequence

<400>  24

Met Gln Ile Tyr Glu Gly Lys Leu Thr Ala Glu Gly Leu Arg Phe Gly 
1               5                   10                  15      


Ile Val Ala Ser Arg Phe Asn His Ala Leu Val Asp Arg Leu Val Glu 
            20                  25                  30          


Gly Ala Ile Asp Ala Ile Val Arg His Gly Gly Arg Glu Glu Asp Ile 
        35                  40                  45              


Thr Leu Val Arg Val Pro Gly Ser Trp Glu Ile Pro Val Ala Ala Gly 
    50                  55                  60                  


Glu Leu Ala Arg Lys Glu Asp Ile Asp Ala Val Ile Ala Ile Gly Val 
65                  70                  75                  80  


Leu Ile Arg Gly Ala Thr Pro His Phe Asp Tyr Ile Ala Ser Glu Val 
                85                  90                  95      


Ser Lys Gly Leu Ala Asp Leu Ser Leu Glu Leu Arg Lys Pro Ile Thr 
            100                 105                 110         


Phe Gly Val Ile Thr Ala Asp Thr Leu Glu Gln Ala Ile Glu Arg Ala 
        115                 120                 125             


Gly Thr Lys His Gly Asn Lys Gly Trp Glu Ala Ala Leu Ser Ala Ile 
    130                 135                 140                 


Glu Met Ala Asn Leu Phe Lys Ser Leu Arg 
145                 150                 


<210>  25
<211>  265
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  self-assembling protein nanoparticle sequence

<400>  25

Met Glu Phe Leu Lys Arg Ser Phe Ala Pro Leu Thr Glu Lys Gln Trp 
1               5                   10                  15      


Gln Glu Ile Asp Asn Arg Ala Arg Glu Ile Phe Lys Thr Gln Leu Tyr 
            20                  25                  30          


Gly Arg Lys Phe Val Asp Val Glu Gly Pro Tyr Gly Trp Glu Tyr Ala 
        35                  40                  45              


Ala His Pro Leu Gly Glu Val Glu Val Leu Ser Asp Glu Asn Glu Val 
    50                  55                  60                  


Val Lys Trp Gly Leu Arg Lys Ser Leu Pro Leu Ile Glu Leu Arg Ala 
65                  70                  75                  80  


Thr Phe Thr Leu Asp Leu Trp Glu Leu Asp Asn Leu Glu Arg Gly Lys 
                85                  90                  95      


Pro Asn Val Asp Leu Ser Ser Leu Glu Glu Thr Val Arg Lys Val Ala 
            100                 105                 110         


Glu Phe Glu Asp Glu Val Ile Phe Arg Gly Cys Glu Lys Ser Gly Val 
        115                 120                 125             


Lys Gly Leu Leu Ser Phe Glu Glu Arg Lys Ile Glu Cys Gly Ser Thr 
    130                 135                 140                 


Pro Lys Asp Leu Leu Glu Ala Ile Val Arg Ala Leu Ser Ile Phe Ser 
145                 150                 155                 160 


Lys Asp Gly Ile Glu Gly Pro Tyr Thr Leu Val Ile Asn Thr Asp Arg 
                165                 170                 175     


Trp Ile Asn Phe Leu Lys Glu Glu Ala Gly His Tyr Pro Leu Glu Lys 
            180                 185                 190         


Arg Val Glu Glu Cys Leu Arg Gly Gly Lys Ile Ile Thr Thr Pro Arg 
        195                 200                 205             


Ile Glu Asp Ala Leu Val Val Ser Glu Arg Gly Gly Asp Phe Lys Leu 
    210                 215                 220                 


Ile Leu Gly Gln Asp Leu Ser Ile Gly Tyr Glu Asp Arg Glu Lys Asp 
225                 230                 235                 240 


Ala Val Arg Leu Phe Ile Thr Glu Thr Phe Thr Phe Gln Val Val Asn 
                245                 250                 255     


Pro Glu Ala Leu Ile Leu Leu Lys Phe 
            260                 265 


<210>  26
<211>  265
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  self-assembling protein nanoparticle sequence

<400>  26

Met Glu Phe Leu Lys Arg Ser Phe Ala Pro Leu Thr Glu Lys Gln Trp 
1               5                   10                  15      


Gln Glu Ile Asp Asn Arg Ala Arg Glu Ile Phe Lys Thr Gln Leu Tyr 
            20                  25                  30          


Gly Arg Lys Phe Val Asp Val Glu Gly Pro Tyr Gly Trp Glu Tyr Ala 
        35                  40                  45              


Ala His Pro Leu Gly Glu Val Glu Val Leu Ser Asp Glu Asn Glu Val 
    50                  55                  60                  


Val Lys Trp Gly Leu Arg Lys Ser Leu Pro Leu Ile Glu Leu Arg Ala 
65                  70                  75                  80  


Thr Phe Thr Leu Asp Leu Trp Glu Leu Asp Asn Leu Glu Arg Gly Lys 
                85                  90                  95      


Pro Asn Val Asp Leu Ser Ser Leu Glu Glu Thr Val Arg Lys Val Ala 
            100                 105                 110         


Glu Phe Glu Asp Glu Val Ile Phe Arg Gly Cys Glu Lys Ser Gly Val 
        115                 120                 125             


Lys Gly Leu Leu Ser Phe Glu Glu Arg Lys Ile Glu Cys Gly Ser Thr 
    130                 135                 140                 


Pro Lys Asp Leu Leu Glu Ala Ile Val Arg Ala Leu Ser Ile Phe Ser 
145                 150                 155                 160 


Lys Asp Gly Ile Glu Gly Pro Tyr Thr Leu Val Ile Asn Thr Asp Arg 
                165                 170                 175     


Trp Ile Asn Phe Leu Lys Glu Glu Ala Gly His Tyr Pro Leu Glu Lys 
            180                 185                 190         


Arg Val Glu Glu Cys Leu Arg Gly Gly Lys Ile Ile Thr Thr Pro Arg 
        195                 200                 205             


Ile Glu Asp Ala Leu Val Val Ser Glu Arg Gly Gly Asp Phe Lys Leu 
    210                 215                 220                 


Ile Leu Gly Gln Asp Leu Ser Ile Gly Tyr Glu Asp Arg Glu Lys Asp 
225                 230                 235                 240 


Ala Val Arg Leu Phe Ile Thr Glu Thr Phe Thr Phe Gln Val Val Asn 
                245                 250                 255     


Pro Glu Ala Leu Ile Leu Leu Lys Phe 
            260                 265 


<210>  27
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GCN4 trimerization domain

<400>  27

Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys 
1               5                   10                  15      


Asp Gly Glu Trp Val Leu Leu Ser Thr Phe 
            20                  25      


<210>  28
<211>  1318
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  28

Met Ile His Ser Val Phe Leu Leu Met Phe Leu Leu Thr Pro Thr Glu 
1               5                   10                  15      


Ser Tyr Val Asp Val Gly Pro Asp Ser Val Lys Ser Ala Cys Ile Glu 
            20                  25                  30          


Val Asp Ile Gln Gln Thr Phe Phe Asp Lys Thr Trp Pro Arg Pro Ile 
        35                  40                  45              


Asp Val Ser Lys Ala Asp Gly Ile Ile Tyr Pro Gln Gly Arg Thr Tyr 
    50                  55                  60                  


Ser Asn Ile Thr Ile Thr Tyr Gln Gly Leu Phe Pro Tyr Gln Gly Asp 
65                  70                  75                  80  


His Gly Asp Met Tyr Val Tyr Ser Ala Gly His Ala Thr Gly Thr Thr 
                85                  90                  95      


Pro Gln Lys Leu Phe Val Ala Asn Tyr Ser Gln Asp Val Lys Gln Phe 
            100                 105                 110         


Ala Asn Gly Phe Val Val Arg Ile Gly Ala Ala Ala Asn Ser Thr Gly 
        115                 120                 125             


Thr Val Ile Ile Ser Pro Ser Thr Ser Ala Thr Ile Arg Lys Ile Tyr 
    130                 135                 140                 


Pro Ala Phe Met Leu Gly Ser Ser Val Gly Asn Phe Ser Asp Gly Lys 
145                 150                 155                 160 


Met Gly Arg Phe Phe Asn His Thr Leu Val Leu Leu Pro Asp Gly Cys 
                165                 170                 175     


Gly Thr Leu Leu Arg Ala Phe Tyr Cys Ile Leu Glu Pro Arg Ser Gly 
            180                 185                 190         


Asn His Cys Pro Ala Gly Asn Ser Tyr Thr Ser Phe Ala Thr Tyr His 
        195                 200                 205             


Thr Pro Ala Thr Asp Cys Ser Asp Gly Asn Tyr Asn Arg Asn Ala Ser 
    210                 215                 220                 


Leu Asn Ser Phe Lys Glu Tyr Phe Asn Leu Arg Asn Cys Thr Phe Met 
225                 230                 235                 240 


Tyr Thr Tyr Asn Ile Thr Glu Asp Glu Ile Leu Glu Trp Phe Gly Ile 
                245                 250                 255     


Thr Gln Thr Ala Gln Gly Val His Leu Phe Ser Ser Arg Tyr Val Asp 
            260                 265                 270         


Leu Tyr Gly Gly Asn Met Phe Gln Phe Ala Thr Leu Pro Val Tyr Asp 
        275                 280                 285             


Thr Ile Lys Tyr Tyr Ser Ile Ile Pro His Ser Ile Arg Ser Ile Gln 
    290                 295                 300                 


Ser Asp Arg Lys Ala Trp Ala Ala Phe Tyr Val Tyr Lys Leu Gln Pro 
305                 310                 315                 320 


Leu Thr Phe Leu Leu Asp Phe Ser Val Asp Gly Tyr Ile Arg Arg Ala 
                325                 330                 335     


Ile Asp Cys Gly Phe Asn Asp Leu Ser Gln Leu His Cys Ser Tyr Glu 
            340                 345                 350         


Ser Phe Asp Val Glu Ser Gly Val Tyr Ser Val Ser Ser Phe Glu Ala 
        355                 360                 365             


Lys Pro Ser Gly Ser Val Val Glu Gln Ala Glu Gly Val Glu Cys Asp 
    370                 375                 380                 


Phe Ser Pro Leu Leu Ser Gly Thr Pro Pro Gln Val Tyr Asn Phe Lys 
385                 390                 395                 400 


Arg Leu Val Phe Thr Asn Cys Asn Tyr Asn Leu Thr Lys Leu Leu Ser 
                405                 410                 415     


Leu Phe Ser Val Asn Asp Phe Thr Cys Ser Gln Ile Ser Pro Ala Ala 
            420                 425                 430         


Ile Ala Ser Asn Cys Tyr Ser Ser Leu Ile Leu Asp Tyr Phe Ser Tyr 
        435                 440                 445             


Pro Leu Ser Met Lys Ser Asp Leu Ser Val Ser Ser Ala Gly Pro Ile 
    450                 455                 460                 


Ser Gln Phe Asn Tyr Lys Gln Ser Phe Ser Asn Pro Thr Cys Leu Ile 
465                 470                 475                 480 


Leu Ala Thr Val Pro His Asn Leu Thr Thr Ile Thr Lys Pro Leu Lys 
                485                 490                 495     


Tyr Ser Tyr Ile Asn Lys Cys Ser Arg Phe Leu Ser Asp Asp Arg Thr 
            500                 505                 510         


Glu Val Pro Gln Leu Val Asn Ala Asn Gln Tyr Ser Pro Cys Val Ser 
        515                 520                 525             


Ile Val Pro Ser Thr Val Trp Glu Asp Gly Asp Tyr Tyr Arg Lys Gln 
    530                 535                 540                 


Leu Ser Pro Leu Glu Gly Gly Gly Trp Leu Val Ala Ser Gly Ser Thr 
545                 550                 555                 560 


Val Ala Met Thr Glu Gln Leu Gln Met Gly Phe Gly Ile Thr Val Gln 
                565                 570                 575     


Tyr Gly Thr Asp Thr Asn Ser Val Cys Pro Lys Leu Glu Phe Ala Asn 
            580                 585                 590         


Asp Thr Lys Ile Ala Ser Gln Leu Gly Asn Cys Val Glu Tyr Ser Leu 
        595                 600                 605             


Tyr Gly Val Ser Gly Arg Gly Val Phe Gln Asn Cys Thr Ala Val Gly 
    610                 615                 620                 


Val Arg Gln Gln Arg Phe Val Tyr Asp Ala Tyr Gln Asn Leu Val Gly 
625                 630                 635                 640 


Tyr Tyr Ser Asp Asp Gly Asn Tyr Tyr Cys Leu Arg Ala Cys Val Ser 
                645                 650                 655     


Val Pro Val Ser Val Ile Tyr Asp Lys Glu Thr Lys Thr His Ala Thr 
            660                 665                 670         


Leu Phe Gly Ser Val Ala Cys Glu His Ile Ser Ser Thr Met Ser Gln 
        675                 680                 685             


Tyr Ser Arg Ser Thr Arg Ser Met Leu Lys Arg Arg Asp Ser Thr Tyr 
    690                 695                 700                 


Gly Pro Leu Gln Thr Pro Val Gly Cys Val Leu Gly Leu Val Asn Ser 
705                 710                 715                 720 


Ser Leu Phe Val Glu Asp Cys Lys Leu Pro Leu Gly Gln Ser Leu Cys 
                725                 730                 735     


Ala Leu Pro Asp Thr Pro Ser Thr Leu Thr Pro Ala Ser Val Gly Ser 
            740                 745                 750         


Val Pro Gly Glu Met Arg Leu Ala Ser Ile Ala Phe Asn His Pro Ile 
        755                 760                 765             


Gln Val Asp Gln Leu Asn Ser Ser Tyr Phe Lys Leu Ser Ile Pro Thr 
    770                 775                 780                 


Asn Phe Ser Phe Gly Val Thr Gln Glu Tyr Ile Gln Thr Thr Ile Gln 
785                 790                 795                 800 


Lys Val Thr Val Asp Cys Lys Gln Tyr Val Cys Asn Gly Phe Gln Lys 
                805                 810                 815     


Cys Glu Gln Leu Leu Arg Glu Tyr Gly Gln Phe Cys Ser Lys Ile Asn 
            820                 825                 830         


Gln Ala Leu His Gly Ala Asn Leu Arg Gln Asp Asp Ser Val Arg Asn 
        835                 840                 845             


Leu Phe Ala Ser Val Lys Ser Ser Gln Ser Ser Pro Ile Ile Pro Gly 
    850                 855                 860                 


Phe Gly Gly Asp Phe Asn Leu Thr Leu Leu Glu Pro Val Ser Ile Ser 
865                 870                 875                 880 


Thr Gly Ser Arg Ser Ala Arg Ser Ala Ile Glu Asp Leu Leu Phe Asp 
                885                 890                 895     


Lys Val Thr Ile Ala Asp Pro Gly Tyr Met Gln Gly Tyr Asp Asp Cys 
            900                 905                 910         


Met Gln Gln Gly Pro Ala Ser Ala Arg Asp Leu Ile Cys Ala Gln Tyr 
        915                 920                 925             


Val Ala Gly Tyr Lys Val Leu Pro Pro Leu Met Asp Val Asn Met Glu 
    930                 935                 940                 


Ala Ala Tyr Thr Ser Ser Leu Leu Gly Ser Ile Ala Gly Val Gly Trp 
945                 950                 955                 960 


Thr Ala Gly Leu Ser Ser Phe Ala Ala Ile Pro Phe Ala Gln Ser Ile 
                965                 970                 975     


Phe Tyr Arg Leu Asn Gly Val Gly Ile Thr Gln Gln Val Leu Ser Glu 
            980                 985                 990         


Asn Gln Lys Leu Ile Ala Asn Lys  Phe Asn Gln Ala Leu  Gly Ala Met 
        995                 1000                 1005             


Gln Thr  Gly Phe Thr Thr Thr  Asn Glu Ala Phe His  Lys Val Gln 
    1010                 1015                 1020             


Asp Ala  Val Asn Asn Asn Ala  Gln Ala Leu Ser Lys  Leu Ala Ser 
    1025                 1030                 1035             


Glu Leu  Ser Asn Thr Phe Gly  Ala Ile Ser Ala Ser  Ile Gly Asp 
    1040                 1045                 1050             


Ile Ile  Gln Arg Leu Asp Pro  Pro Glu Gln Asp Ala  Gln Ile Asp 
    1055                 1060                 1065             


Arg Leu  Ile Asn Gly Arg Leu  Thr Thr Leu Asn Ala  Phe Val Ala 
    1070                 1075                 1080             


Gln Gln  Leu Val Arg Ser Glu  Ser Ala Ala Leu Ser  Ala Gln Leu 
    1085                 1090                 1095             


Ala Lys  Asp Lys Val Asn Glu  Cys Val Lys Ala Gln  Ser Lys Arg 
    1100                 1105                 1110             


Ser Gly  Phe Cys Gly Gln Gly  Thr His Ile Val Ser  Phe Val Val 
    1115                 1120                 1125             


Asn Ala  Pro Asn Gly Leu Tyr  Phe Met His Val Gly  Tyr Tyr Pro 
    1130                 1135                 1140             


Ser Asn  His Ile Glu Val Val  Ser Ala Tyr Gly Leu  Cys Asp Ala 
    1145                 1150                 1155             


Ala Asn  Pro Thr Asn Cys Ile  Ala Pro Val Asn Gly  Tyr Phe Ile 
    1160                 1165                 1170             


Lys Thr  Asn Asn Thr Arg Ile  Val Asp Glu Trp Ser  Tyr Thr Gly 
    1175                 1180                 1185             


Ser Ser  Phe Tyr Ala Pro Glu  Pro Ile Thr Ser Leu  Asn Thr Lys 
    1190                 1195                 1200             


Tyr Val  Ala Pro Gln Val Thr  Tyr Gln Asn Ile Ser  Thr Asn Leu 
    1205                 1210                 1215             


Pro Pro  Pro Leu Leu Gly Asn  Ser Thr Gly Ile Asp  Phe Gln Asp 
    1220                 1225                 1230             


Glu Leu  Asp Glu Phe Phe Lys  Asn Val Ser Thr Ser  Ile Pro Asn 
    1235                 1240                 1245             


Phe Gly  Ser Leu Thr Gln Ile  Asn Thr Thr Leu Leu  Asp Leu Thr 
    1250                 1255                 1260             


Tyr Glu  Met Leu Ser Leu Gln  Gln Val Val Lys Ala  Leu Asn Glu 
    1265                 1270                 1275             


Ser Tyr  Ile Asp Leu Lys Glu  Leu Gly Asn Tyr Thr  Tyr Gly Gly 
    1280                 1285                 1290             


Tyr Ile  Pro Glu Ala Pro Arg  Asp Gly Gln Ala Tyr  Val Arg Lys 
    1295                 1300                 1305             


Asp Gly  Glu Trp Val Leu Leu  Ser Thr Phe 
    1310                 1315             


<210>  29
<211>  1318
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  29

Met Ile His Ser Val Phe Leu Leu Met Phe Leu Leu Thr Pro Thr Glu 
1               5                   10                  15      


Ser Tyr Val Asp Val Gly Pro Asp Ser Val Lys Ser Ala Cys Ile Glu 
            20                  25                  30          


Val Asp Ile Gln Gln Thr Phe Phe Asp Lys Thr Trp Pro Arg Pro Ile 
        35                  40                  45              


Asp Val Ser Lys Ala Asp Gly Ile Ile Tyr Pro Gln Gly Arg Thr Tyr 
    50                  55                  60                  


Ser Asn Ile Thr Ile Thr Tyr Gln Gly Leu Phe Pro Tyr Gln Gly Asp 
65                  70                  75                  80  


His Gly Asp Met Tyr Val Tyr Ser Ala Gly His Ala Thr Gly Thr Thr 
                85                  90                  95      


Pro Gln Lys Leu Phe Val Ala Asn Tyr Ser Gln Asp Val Lys Gln Phe 
            100                 105                 110         


Ala Asn Gly Phe Val Val Arg Ile Gly Ala Ala Ala Asn Ser Thr Gly 
        115                 120                 125             


Thr Val Ile Ile Ser Pro Ser Thr Ser Ala Thr Ile Arg Lys Ile Tyr 
    130                 135                 140                 


Pro Ala Phe Met Leu Gly Ser Ser Val Gly Asn Phe Ser Asp Gly Lys 
145                 150                 155                 160 


Met Gly Arg Phe Phe Asn His Thr Leu Val Leu Leu Pro Asp Gly Cys 
                165                 170                 175     


Gly Thr Leu Leu Arg Ala Phe Tyr Cys Ile Leu Glu Pro Arg Ser Gly 
            180                 185                 190         


Asn His Cys Pro Ala Gly Asn Ser Tyr Thr Ser Phe Ala Thr Tyr His 
        195                 200                 205             


Thr Pro Ala Thr Asp Cys Ser Asp Gly Asn Tyr Asn Arg Asn Ala Ser 
    210                 215                 220                 


Leu Asn Ser Phe Lys Glu Tyr Phe Asn Leu Arg Asn Cys Thr Phe Met 
225                 230                 235                 240 


Tyr Thr Tyr Asn Ile Thr Glu Asp Glu Ile Leu Glu Trp Phe Gly Ile 
                245                 250                 255     


Thr Gln Thr Ala Gln Gly Val His Leu Phe Ser Ser Arg Tyr Val Asp 
            260                 265                 270         


Leu Tyr Gly Gly Asn Met Phe Gln Phe Ala Thr Leu Pro Val Tyr Asp 
        275                 280                 285             


Thr Ile Lys Tyr Tyr Ser Ile Ile Pro His Ser Ile Arg Ser Ile Gln 
    290                 295                 300                 


Ser Asp Arg Lys Ala Trp Ala Ala Phe Tyr Val Tyr Lys Leu Gln Pro 
305                 310                 315                 320 


Leu Thr Phe Leu Leu Asp Phe Ser Val Asp Gly Tyr Ile Arg Arg Ala 
                325                 330                 335     


Ile Asp Cys Gly Phe Asn Asp Leu Ser Gln Leu His Cys Ser Tyr Glu 
            340                 345                 350         


Ser Phe Asp Val Glu Ser Gly Val Tyr Ser Val Ser Ser Phe Glu Ala 
        355                 360                 365             


Lys Pro Ser Gly Ser Val Val Glu Gln Ala Glu Gly Val Glu Cys Asp 
    370                 375                 380                 


Phe Ser Pro Leu Leu Ser Gly Thr Pro Pro Gln Val Tyr Asn Phe Lys 
385                 390                 395                 400 


Arg Leu Val Phe Thr Asn Cys Asn Tyr Asn Leu Thr Lys Leu Leu Ser 
                405                 410                 415     


Leu Phe Ser Val Asn Asp Phe Thr Cys Ser Gln Ile Ser Pro Ala Ala 
            420                 425                 430         


Ile Ala Ser Asn Cys Tyr Ser Ser Leu Ile Leu Asp Tyr Phe Ser Tyr 
        435                 440                 445             


Pro Leu Ser Met Lys Ser Asp Leu Ser Val Ser Ser Ala Gly Pro Ile 
    450                 455                 460                 


Ser Gln Phe Asn Tyr Lys Gln Ser Phe Ser Asn Pro Thr Cys Leu Ile 
465                 470                 475                 480 


Leu Ala Thr Val Pro His Asn Leu Thr Thr Ile Thr Lys Pro Leu Lys 
                485                 490                 495     


Tyr Ser Tyr Ile Asn Lys Cys Ser Arg Phe Leu Ser Asp Asp Arg Thr 
            500                 505                 510         


Glu Val Pro Gln Leu Val Asn Ala Asn Gln Tyr Ser Pro Cys Val Ser 
        515                 520                 525             


Ile Val Pro Ser Thr Val Trp Glu Asp Gly Asp Tyr Tyr Arg Lys Gln 
    530                 535                 540                 


Leu Ser Pro Leu Glu Gly Gly Gly Trp Leu Val Ala Ser Gly Ser Thr 
545                 550                 555                 560 


Val Ala Met Thr Glu Gln Leu Gln Met Gly Phe Gly Ile Thr Val Gln 
                565                 570                 575     


Tyr Gly Thr Asp Thr Asn Ser Val Cys Pro Lys Leu Glu Phe Ala Asn 
            580                 585                 590         


Asp Thr Lys Ile Ala Ser Gln Leu Gly Asn Cys Val Glu Tyr Ser Leu 
        595                 600                 605             


Tyr Gly Val Ser Gly Arg Gly Val Phe Gln Asn Cys Thr Ala Val Gly 
    610                 615                 620                 


Val Arg Gln Gln Arg Phe Val Tyr Asp Ala Tyr Gln Asn Leu Val Gly 
625                 630                 635                 640 


Tyr Tyr Ser Asp Asp Gly Asn Tyr Tyr Cys Leu Arg Ala Cys Val Ser 
                645                 650                 655     


Val Pro Val Ser Val Ile Tyr Asp Lys Glu Thr Lys Thr His Ala Thr 
            660                 665                 670         


Leu Phe Gly Ser Val Ala Cys Glu His Ile Ser Ser Thr Met Ser Gln 
        675                 680                 685             


Tyr Ser Arg Ser Thr Arg Ser Met Leu Lys Arg Arg Asp Ser Thr Tyr 
    690                 695                 700                 


Gly Pro Leu Gln Thr Pro Val Gly Cys Val Leu Gly Leu Val Asn Ser 
705                 710                 715                 720 


Ser Leu Phe Val Glu Asp Cys Lys Leu Pro Leu Gly Gln Ser Leu Cys 
                725                 730                 735     


Ala Leu Pro Asp Thr Pro Ser Thr Leu Thr Pro Ala Ser Val Gly Ser 
            740                 745                 750         


Val Pro Gly Glu Met Arg Leu Ala Ser Ile Ala Phe Asn His Pro Ile 
        755                 760                 765             


Gln Val Asp Gln Leu Asn Ser Ser Tyr Phe Lys Leu Ser Ile Pro Thr 
    770                 775                 780                 


Asn Phe Ser Phe Gly Val Thr Gln Glu Tyr Ile Gln Thr Thr Ile Gln 
785                 790                 795                 800 


Lys Val Thr Val Asp Cys Lys Gln Tyr Val Cys Asn Gly Phe Gln Lys 
                805                 810                 815     


Cys Glu Gln Leu Leu Arg Glu Tyr Gly Gln Phe Cys Ser Lys Ile Asn 
            820                 825                 830         


Gln Ala Leu His Gly Ala Asn Leu Arg Gln Asp Asp Ser Val Arg Asn 
        835                 840                 845             


Leu Phe Ala Ser Val Lys Ser Ser Gln Ser Ser Pro Ile Ile Pro Gly 
    850                 855                 860                 


Phe Gly Gly Asp Phe Asn Leu Thr Leu Leu Glu Pro Val Ser Ile Ser 
865                 870                 875                 880 


Thr Gly Ser Gly Ser Ala Gly Ser Ala Ile Glu Asp Leu Leu Phe Asp 
                885                 890                 895     


Lys Val Thr Ile Ala Asp Pro Gly Tyr Met Gln Gly Tyr Asp Asp Cys 
            900                 905                 910         


Met Gln Gln Gly Pro Ala Ser Ala Arg Asp Leu Ile Cys Ala Gln Tyr 
        915                 920                 925             


Val Ala Gly Tyr Lys Val Leu Pro Pro Leu Met Asp Val Asn Met Glu 
    930                 935                 940                 


Ala Ala Tyr Thr Ser Ser Leu Leu Gly Ser Ile Ala Gly Val Gly Trp 
945                 950                 955                 960 


Thr Ala Gly Leu Ser Ser Phe Ala Ala Ile Pro Phe Ala Gln Ser Ile 
                965                 970                 975     


Phe Tyr Arg Leu Asn Gly Val Gly Ile Thr Gln Gln Val Leu Ser Glu 
            980                 985                 990         


Asn Gln Lys Leu Ile Ala Asn Lys  Phe Asn Gln Ala Leu  Gly Ala Met 
        995                 1000                 1005             


Gln Thr  Gly Phe Thr Thr Thr  Asn Glu Ala Phe His  Lys Val Gln 
    1010                 1015                 1020             


Asp Ala  Val Asn Asn Asn Ala  Gln Ala Leu Ser Lys  Leu Ala Ser 
    1025                 1030                 1035             


Glu Leu  Ser Asn Thr Phe Gly  Ala Ile Ser Ala Ser  Ile Gly Asp 
    1040                 1045                 1050             


Ile Ile  Gln Arg Leu Asp Pro  Pro Glu Gln Asp Ala  Gln Ile Asp 
    1055                 1060                 1065             


Arg Leu  Ile Asn Gly Arg Leu  Thr Thr Leu Asn Ala  Phe Val Ala 
    1070                 1075                 1080             


Gln Gln  Leu Val Arg Ser Glu  Ser Ala Ala Leu Ser  Ala Gln Leu 
    1085                 1090                 1095             


Ala Lys  Asp Lys Val Asn Glu  Cys Val Lys Ala Gln  Ser Lys Arg 
    1100                 1105                 1110             


Ser Gly  Phe Cys Gly Gln Gly  Thr His Ile Val Ser  Phe Val Val 
    1115                 1120                 1125             


Asn Ala  Pro Asn Gly Leu Tyr  Phe Met His Val Gly  Tyr Tyr Pro 
    1130                 1135                 1140             


Ser Asn  His Ile Glu Val Val  Ser Ala Tyr Gly Leu  Cys Asp Ala 
    1145                 1150                 1155             


Ala Asn  Pro Thr Asn Cys Ile  Ala Pro Val Asn Gly  Tyr Phe Ile 
    1160                 1165                 1170             


Lys Thr  Asn Asn Thr Arg Ile  Val Asp Glu Trp Ser  Tyr Thr Gly 
    1175                 1180                 1185             


Ser Ser  Phe Tyr Ala Pro Glu  Pro Ile Thr Ser Leu  Asn Thr Lys 
    1190                 1195                 1200             


Tyr Val  Ala Pro Gln Val Thr  Tyr Gln Asn Ile Ser  Thr Asn Leu 
    1205                 1210                 1215             


Pro Pro  Pro Leu Leu Gly Asn  Ser Thr Gly Ile Asp  Phe Gln Asp 
    1220                 1225                 1230             


Glu Leu  Asp Glu Phe Phe Lys  Asn Val Ser Thr Ser  Ile Pro Asn 
    1235                 1240                 1245             


Phe Gly  Ser Leu Thr Gln Ile  Asn Thr Thr Leu Leu  Asp Leu Thr 
    1250                 1255                 1260             


Tyr Glu  Met Leu Ser Leu Gln  Gln Val Val Lys Ala  Leu Asn Glu 
    1265                 1270                 1275             


Ser Tyr  Ile Asp Leu Lys Glu  Leu Gly Asn Tyr Thr  Tyr Gly Gly 
    1280                 1285                 1290             


Tyr Ile  Pro Glu Ala Pro Arg  Asp Gly Gln Ala Tyr  Val Arg Lys 
    1295                 1300                 1305             


Asp Gly  Glu Trp Val Leu Leu  Ser Thr Phe 
    1310                 1315             


<210>  30
<211>  1217
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  30

Met Phe Ile Phe Leu Leu Phe Leu Thr Leu Thr Ser Gly Ser Asp Leu 
1               5                   10                  15      


Asp Arg Cys Thr Thr Phe Asp Asp Val Gln Ala Pro Asn Tyr Thr Gln 
            20                  25                  30          


His Thr Ser Ser Met Arg Gly Val Tyr Tyr Pro Asp Glu Ile Phe Arg 
        35                  40                  45              


Ser Asp Thr Leu Tyr Leu Thr Gln Asp Leu Phe Leu Pro Phe Tyr Ser 
    50                  55                  60                  


Asn Val Thr Gly Phe His Thr Ile Asn His Thr Phe Gly Asn Pro Val 
65                  70                  75                  80  


Ile Pro Phe Lys Asp Gly Ile Tyr Phe Ala Ala Thr Glu Lys Ser Asn 
                85                  90                  95      


Val Val Arg Gly Trp Val Phe Gly Ser Thr Met Asn Asn Lys Ser Gln 
            100                 105                 110         


Ser Val Ile Ile Ile Asn Asn Ser Thr Asn Val Val Ile Arg Ala Cys 
        115                 120                 125             


Asn Phe Glu Leu Cys Asp Asn Pro Phe Phe Ala Val Ser Lys Pro Met 
    130                 135                 140                 


Gly Thr Gln Thr His Thr Met Ile Phe Asp Asn Ala Phe Asn Cys Thr 
145                 150                 155                 160 


Phe Glu Tyr Ile Ser Asp Ala Phe Ser Leu Asp Val Ser Glu Lys Ser 
                165                 170                 175     


Gly Asn Phe Lys His Leu Arg Glu Phe Val Phe Lys Asn Lys Asp Gly 
            180                 185                 190         


Phe Leu Tyr Val Tyr Lys Gly Tyr Gln Pro Ile Asp Val Val Arg Asp 
        195                 200                 205             


Leu Pro Ser Gly Phe Asn Thr Leu Lys Pro Ile Phe Lys Leu Pro Leu 
    210                 215                 220                 


Gly Ile Asn Ile Thr Asn Phe Arg Ala Ile Leu Thr Ala Phe Ser Pro 
225                 230                 235                 240 


Ala Gln Asp Ile Trp Gly Thr Ser Ala Ala Ala Tyr Phe Val Gly Tyr 
                245                 250                 255     


Leu Lys Pro Thr Thr Phe Met Leu Lys Tyr Asp Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ser Gln Asn Pro Leu Ala Glu Leu Lys Cys 
        275                 280                 285             


Ser Val Lys Ser Phe Glu Ile Asp Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Val Pro Ser Gly Asp Val Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Pro Ser 
                325                 330                 335     


Val Tyr Ala Trp Glu Arg Lys Lys Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Thr Phe Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Met Gly Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr Ser 
            420                 425                 430         


Thr Gly Asn Tyr Asn Tyr Lys Tyr Arg Tyr Leu Arg His Gly Lys Leu 
        435                 440                 445             


Arg Pro Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp Gly 
    450                 455                 460                 


Lys Pro Cys Thr Pro Pro Ala Leu Asn Cys Tyr Trp Pro Leu Asn Asp 
465                 470                 475                 480 


Tyr Gly Phe Tyr Thr Thr Thr Gly Ile Gly Tyr Gln Pro Tyr Arg Val 
                485                 490                 495     


Val Val Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys Gly 
            500                 505                 510         


Pro Lys Leu Ser Thr Asp Leu Ile Lys Asn Gln Cys Val Asn Phe Asn 
        515                 520                 525             


Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Pro Ser Ser Lys Arg 
    530                 535                 540                 


Phe Gln Pro Phe Gln Gln Phe Gly Arg Asp Val Ser Asp Phe Thr Asp 
545                 550                 555                 560 


Ser Val Arg Asp Pro Lys Thr Ser Glu Ile Leu Asp Ile Ser Pro Cys 
                565                 570                 575     


Ala Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Ala Ser Ser 
            580                 585                 590         


Glu Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Asp Val Ser Thr 
        595                 600                 605             


Ala Ile His Ala Asp Gln Leu Thr Pro Ala Trp Arg Ile Tyr Ser Thr 
    610                 615                 620                 


Gly Asn Asn Val Phe Gln Thr Gln Ala Gly Cys Leu Ile Gly Ala Glu 
625                 630                 635                 640 


His Val Asp Thr Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile 
                645                 650                 655     


Cys Ala Ser Tyr His Thr Val Ser Leu Leu Arg Ser Thr Ser Gln Lys 
            660                 665                 670         


Ser Ile Val Ala Tyr Thr Met Ser Leu Gly Ala Asp Ser Ser Ile Ala 
        675                 680                 685             


Tyr Ser Asn Asn Thr Ile Ala Ile Pro Thr Asn Phe Ser Ile Ser Ile 
    690                 695                 700                 


Thr Thr Glu Val Met Pro Val Ser Met Ala Lys Thr Ser Val Asp Cys 
705                 710                 715                 720 


Asn Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ala Asn Leu Leu Leu 
                725                 730                 735     


Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Ser Gly Ile 
            740                 745                 750         


Ala Ala Glu Gln Asp Arg Asn Thr Arg Glu Val Phe Ala Gln Val Lys 
        755                 760                 765             


Gln Met Tyr Lys Thr Pro Thr Leu Lys Tyr Phe Gly Gly Phe Asn Phe 
    770                 775                 780                 


Ser Gln Ile Leu Pro Asp Pro Leu Lys Pro Thr Lys Arg Ser Phe Ile 
785                 790                 795                 800 


Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Met 
                805                 810                 815     


Lys Gln Tyr Gly Glu Cys Leu Gly Asp Ile Asn Ala Arg Asp Leu Ile 
            820                 825                 830         


Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr 
        835                 840                 845             


Asp Asp Met Ile Ala Ala Tyr Thr Ala Ala Leu Val Ser Gly Thr Ala 
    850                 855                 860                 


Thr Ala Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe 
865                 870                 875                 880 


Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn 
                885                 890                 895     


Val Leu Tyr Glu Asn Gln Lys Gln Ile Ala Asn Gln Phe Asn Lys Ala 
            900                 905                 910         


Ile Ser Gln Ile Gln Glu Ser Leu Thr Thr Thr Ser Thr Ala Leu Gly 
        915                 920                 925             


Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu 
    930                 935                 940                 


Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn 
945                 950                 955                 960 


Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp 
                965                 970                 975     


Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln 
            980                 985                 990         


Gln Leu Ile Arg Ala Ala Glu Ile  Arg Ala Ser Ala Asn  Leu Ala Ala 
        995                 1000                 1005             


Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp 
    1010                 1015                 1020             


Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser Phe Pro  Gln Ala Ala 
    1025                 1030                 1035             


Pro His  Gly Val Val Phe Leu  His Val Thr Tyr Val  Pro Ser Gln 
    1040                 1045                 1050             


Glu Arg  Asn Phe Thr Thr Ala  Pro Ala Ile Cys His  Glu Gly Lys 
    1055                 1060                 1065             


Ala Tyr  Phe Pro Arg Glu Gly  Val Phe Val Phe Asn  Gly Thr Ser 
    1070                 1075                 1080             


Trp Phe  Ile Thr Gln Arg Asn  Phe Phe Ser Pro Gln  Ile Ile Thr 
    1085                 1090                 1095             


Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys Asp Val  Val Ile Gly 
    1100                 1105                 1110             


Ile Ile  Asn Asn Thr Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp 
    1115                 1120                 1125             


Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser 
    1130                 1135                 1140             


Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val 
    1145                 1150                 1155             


Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys 
    1160                 1165                 1170             


Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr 
    1175                 1180                 1185             


Glu Gln  Gly Gly Tyr Ile Pro  Glu Ala Pro Arg Asp  Gly Gln Ala 
    1190                 1195                 1200             


Tyr Val  Arg Lys Asp Gly Glu  Trp Val Leu Leu Ser  Thr Phe 
    1205                 1210                 1215         


<210>  31
<211>  1303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  31

Met Phe Leu Ile Ile Phe Ile Leu Pro Thr Thr Leu Ala Val Ile Gly 
1               5                   10                  15      


Asp Phe Asn Cys Thr Asn Ser Phe Ile Asn Asp Tyr Asn Lys Thr Ile 
            20                  25                  30          


Pro Arg Ile Ser Glu Asp Val Val Asp Val Ser Leu Gly Leu Gly Thr 
        35                  40                  45              


Tyr Tyr Val Leu Asn Arg Val Tyr Leu Asn Thr Thr Leu Leu Phe Thr 
    50                  55                  60                  


Gly Tyr Phe Pro Lys Ser Gly Ala Asn Phe Arg Asp Leu Ala Leu Lys 
65                  70                  75                  80  


Gly Ser Ile Tyr Leu Ser Thr Leu Trp Tyr Lys Pro Pro Phe Leu Ser 
                85                  90                  95      


Asp Phe Asn Asn Gly Ile Phe Ser Lys Val Lys Asn Thr Lys Leu Tyr 
            100                 105                 110         


Val Asn Asn Thr Leu Tyr Ser Glu Phe Ser Thr Ile Val Ile Gly Ser 
        115                 120                 125             


Val Phe Val Asn Thr Ser Tyr Thr Ile Val Val Gln Pro His Asn Gly 
    130                 135                 140                 


Ile Leu Glu Ile Thr Ala Cys Gln Tyr Thr Met Cys Glu Tyr Pro His 
145                 150                 155                 160 


Thr Val Cys Lys Ser Lys Gly Ser Ile Arg Asn Glu Ser Trp His Ile 
                165                 170                 175     


Asp Ser Ser Glu Pro Leu Cys Leu Phe Lys Lys Asn Phe Thr Tyr Asn 
            180                 185                 190         


Val Ser Ala Asp Trp Leu Tyr Phe His Phe Tyr Gln Glu Arg Gly Val 
        195                 200                 205             


Phe Tyr Ala Tyr Tyr Ala Asp Val Gly Met Pro Thr Thr Phe Leu Phe 
    210                 215                 220                 


Ser Leu Tyr Leu Gly Thr Ile Leu Ser His Tyr Tyr Val Met Pro Leu 
225                 230                 235                 240 


Thr Cys Asn Ala Ile Ser Ser Asn Thr Asp Asn Glu Thr Leu Glu Tyr 
                245                 250                 255     


Trp Val Thr Pro Leu Ser Arg Arg Gln Tyr Leu Leu Asn Phe Asp Glu 
            260                 265                 270         


His Gly Val Ile Thr Asn Ala Val Asp Cys Ser Ser Ser Phe Leu Ser 
        275                 280                 285             


Glu Ile Gln Cys Lys Thr Gln Ser Phe Ala Pro Asn Thr Gly Val Tyr 
    290                 295                 300                 


Asp Leu Ser Gly Phe Thr Val Lys Pro Val Ala Thr Val Tyr Arg Arg 
305                 310                 315                 320 


Ile Pro Asn Leu Pro Asp Cys Asp Ile Asp Asn Trp Leu Asn Asn Val 
                325                 330                 335     


Ser Val Pro Ser Pro Leu Asn Trp Glu Arg Arg Ile Phe Ser Asn Cys 
            340                 345                 350         


Asn Phe Asn Leu Ser Thr Leu Leu Arg Leu Val His Val Asp Ser Phe 
        355                 360                 365             


Ser Cys Asn Asn Leu Asp Lys Ser Lys Ile Phe Gly Ser Cys Phe Asn 
    370                 375                 380                 


Ser Ile Thr Val Asp Lys Phe Ala Ile Pro Asn Arg Arg Arg Asp Asp 
385                 390                 395                 400 


Leu Gln Leu Gly Ser Ser Gly Phe Leu Gln Ser Ser Asn Tyr Lys Ile 
                405                 410                 415     


Asp Ile Ser Ser Ser Ser Cys Gln Leu Tyr Tyr Ser Leu Pro Leu Val 
            420                 425                 430         


Asn Val Thr Ile Asn Asn Phe Asn Pro Ser Ser Trp Asn Arg Arg Tyr 
        435                 440                 445             


Gly Phe Gly Ser Phe Asn Leu Ser Ser Tyr Asp Val Val Tyr Ser Asp 
    450                 455                 460                 


His Cys Phe Ser Val Asn Ser Asp Phe Cys Pro Cys Ala Asp Pro Ser 
465                 470                 475                 480 


Val Val Asn Ser Cys Ala Lys Ser Lys Pro Pro Ser Ala Ile Cys Pro 
                485                 490                 495     


Ala Gly Thr Lys Tyr Arg His Cys Asp Leu Asp Thr Thr Leu Tyr Val 
            500                 505                 510         


Lys Asn Trp Cys Arg Cys Ser Cys Leu Pro Asp Pro Ile Ser Thr Tyr 
        515                 520                 525             


Ser Pro Asn Thr Cys Pro Gln Lys Lys Val Val Val Gly Ile Gly Glu 
    530                 535                 540                 


His Cys Pro Gly Leu Gly Ile Asn Glu Glu Lys Cys Gly Thr Gln Leu 
545                 550                 555                 560 


Asn His Ser Ser Cys Phe Cys Ser Pro Asp Ala Phe Leu Gly Trp Ser 
                565                 570                 575     


Phe Asp Ser Cys Ile Ser Asn Asn Arg Cys Asn Ile Phe Ser Asn Phe 
            580                 585                 590         


Ile Phe Asn Gly Ile Asn Ser Gly Thr Thr Cys Ser Asn Asp Leu Leu 
        595                 600                 605             


Tyr Ser Asn Thr Glu Ile Ser Thr Gly Val Cys Val Asn Tyr Asp Leu 
    610                 615                 620                 


Tyr Gly Ile Thr Gly Gln Gly Ile Phe Lys Glu Val Ser Ala Ala Tyr 
625                 630                 635                 640 


Tyr Asn Asn Trp Gln Asn Leu Leu Tyr Asp Ser Asn Gly Asn Ile Ile 
                645                 650                 655     


Gly Phe Lys Asp Phe Leu Thr Asn Lys Thr Tyr Thr Ile Leu Pro Cys 
            660                 665                 670         


Tyr Ser Gly Arg Val Ser Ala Ala Phe Tyr Gln Asn Ser Ser Ser Pro 
        675                 680                 685             


Ala Leu Leu Tyr Arg Asn Leu Lys Cys Ser Tyr Val Leu Asn Asn Ile 
    690                 695                 700                 


Ser Phe Ile Ser Gln Pro Phe Tyr Phe Asp Ser Tyr Leu Gly Cys Val 
705                 710                 715                 720 


Leu Asn Ala Val Asn Leu Thr Ser Tyr Ser Val Ser Ser Cys Asp Leu 
                725                 730                 735     


Arg Met Gly Ser Gly Phe Cys Ile Asp Tyr Ala Leu Pro Ser Ser Gly 
            740                 745                 750         


Gly Ser Gly Ser Gly Ile Ser Ser Pro Tyr Arg Phe Val Thr Phe Glu 
        755                 760                 765             


Pro Phe Asn Val Ser Phe Val Asn Asp Ser Val Glu Thr Val Gly Gly 
    770                 775                 780                 


Leu Phe Glu Ile Gln Ile Pro Thr Asn Phe Thr Ile Ala Gly His Glu 
785                 790                 795                 800 


Glu Phe Ile Gln Thr Ser Ser Pro Lys Val Thr Ile Asp Cys Ser Ala 
                805                 810                 815     


Phe Val Cys Ser Asn Tyr Ala Ala Cys His Asp Leu Leu Ser Glu Tyr 
            820                 825                 830         


Gly Thr Phe Cys Asp Asn Ile Asn Ser Ile Leu Asn Glu Val Asn Asp 
        835                 840                 845             


Leu Leu Asp Ile Thr Gln Leu Gln Val Ala Asn Ala Leu Met Gln Gly 
    850                 855                 860                 


Val Thr Leu Ser Ser Asn Leu Asn Thr Asn Leu His Ser Asp Val Asp 
865                 870                 875                 880 


Asn Ile Asp Phe Lys Ser Leu Leu Gly Cys Leu Gly Ser Gln Cys Gly 
                885                 890                 895     


Ser Ser Ser Arg Ser Leu Leu Glu Asp Leu Leu Phe Asn Lys Val Lys 
            900                 905                 910         


Leu Ser Asp Val Gly Phe Val Glu Ala Tyr Asn Asn Cys Thr Gly Gly 
        915                 920                 925             


Ser Glu Ile Arg Asp Leu Leu Cys Val Gln Ser Phe Asn Gly Ile Lys 
    930                 935                 940                 


Val Leu Pro Pro Ile Leu Ser Glu Thr Gln Ile Ser Gly Tyr Thr Thr 
945                 950                 955                 960 


Ala Ala Thr Val Ala Ala Met Phe Pro Pro Trp Ser Ala Ala Ala Gly 
                965                 970                 975     


Val Pro Phe Ser Leu Asn Val Gln Tyr Arg Ile Asn Gly Leu Gly Val 
            980                 985                 990         


Thr Met Asp Val Leu Asn Lys Asn  Gln Lys Leu Ile Ala  Asn Ala Phe 
        995                 1000                 1005             


Asn Lys  Ala Leu Leu Ser Ile  Gln Asn Gly Phe Thr  Ala Thr Asn 
    1010                 1015                 1020             


Ser Ala  Leu Ala Lys Ile Gln  Ser Val Val Asn Ala  Asn Ala Gln 
    1025                 1030                 1035             


Ala Leu  Asn Ser Leu Leu Gln  Gln Leu Phe Asn Lys  Phe Gly Ala 
    1040                 1045                 1050             


Ile Ser  Ser Ser Leu Gln Glu  Ile Leu Ser Arg Leu  Asp Pro Pro 
    1055                 1060                 1065             


Glu Ala  Gln Val Gln Ile Asp  Arg Leu Ile Asn Gly  Arg Leu Thr 
    1070                 1075                 1080             


Ala Leu  Asn Ala Tyr Val Ser  Gln Gln Leu Ser Asp  Ile Thr Leu 
    1085                 1090                 1095             


Ile Lys  Ala Gly Ala Ser Arg  Ala Ile Glu Lys Val  Asn Glu Cys 
    1100                 1105                 1110             


Val Lys  Ser Gln Ser Pro Arg  Ile Asn Phe Cys Gly  Asn Gly Asn 
    1115                 1120                 1125             


His Ile  Leu Ser Leu Val Gln  Asn Ala Pro Tyr Gly  Leu Leu Phe 
    1130                 1135                 1140             


Ile His  Phe Ser Tyr Lys Pro  Thr Ser Phe Lys Thr  Val Leu Val 
    1145                 1150                 1155             


Ser Pro  Gly Leu Cys Leu Ser  Gly Asp Arg Gly Ile  Ala Pro Lys 
    1160                 1165                 1170             


Gln Gly  Tyr Phe Ile Lys Gln  Asn Asp Ser Trp Met  Phe Thr Gly 
    1175                 1180                 1185             


Ser Ser  Tyr Tyr Tyr Pro Glu  Pro Ile Ser Asp Lys  Asn Val Val 
    1190                 1195                 1200             


Phe Met  Asn Ser Cys Ser Val  Asn Phe Thr Lys Ala  Pro Phe Ile 
    1205                 1210                 1215             


Tyr Leu  Asn Asn Ser Ile Pro  Asn Leu Ser Asp Phe  Glu Ala Glu 
    1220                 1225                 1230             


Leu Ser  Leu Trp Phe Lys Asn  His Thr Ser Ile Ala  Pro Asn Leu 
    1235                 1240                 1245             


Thr Phe  Asn Ser His Ile Asn  Ala Thr Phe Leu Asp  Leu Tyr Tyr 
    1250                 1255                 1260             


Glu Met  Asn Val Ile Gln Glu  Ser Ile Lys Ser Leu  Asn Gly Gly 
    1265                 1270                 1275             


Tyr Ile  Pro Glu Ala Pro Arg  Asp Gly Gln Ala Tyr  Val Arg Lys 
    1280                 1285                 1290             


Asp Gly  Glu Trp Val Leu Leu  Ser Thr Phe 
    1295                 1300             


<210>  32
<211>  1234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  32

Met Leu Leu Ile Leu Val Leu Gly Val Ser Leu Ala Ala Ala Ser Arg 
1               5                   10                  15      


Pro Glu Cys Phe Asn Pro Arg Phe Thr Leu Thr Pro Leu Asn His Thr 
            20                  25                  30          


Leu Asn Tyr Thr Ser Ile Lys Ala Lys Val Ser Asn Val Leu Leu Pro 
        35                  40                  45              


Asp Pro Tyr Ile Ala Tyr Ser Gly Gln Thr Leu Arg Gln Asn Leu Phe 
    50                  55                  60                  


Met Ala Asp Met Ser Asn Thr Ile Leu Tyr Pro Val Thr Pro Pro Ala 
65                  70                  75                  80  


Asn Gly Ala Asn Gly Gly Phe Ile Tyr Asn Thr Ser Ile Ile Pro Val 
                85                  90                  95      


Ser Ala Gly Leu Phe Val Asn Thr Trp Met Tyr Arg Gln Pro Ala Ser 
            100                 105                 110         


Ser Arg Ala Tyr Cys Gln Glu Pro Phe Gly Val Ala Phe Gly Asp Thr 
        115                 120                 125             


Phe Glu Asn Asp Arg Ile Ala Ile Leu Ile Met Ala Pro Asp Asn Leu 
    130                 135                 140                 


Gly Ser Trp Ser Ala Val Ala Pro Arg Asn Gln Thr Asn Ile Tyr Leu 
145                 150                 155                 160 


Leu Val Cys Ser Asn Ala Thr Leu Cys Ile Asn Pro Gly Phe Asn Arg 
                165                 170                 175     


Trp Gly Pro Ala Gly Ser Phe Ile Ala Pro Asp Ala Leu Val Asp His 
            180                 185                 190         


Ser Asn Ser Cys Phe Val Asn Asn Thr Phe Ser Val Asn Ile Ser Thr 
        195                 200                 205             


Ser Arg Ile Ser Leu Ala Phe Leu Phe Lys Asp Gly Asp Leu Leu Ile 
    210                 215                 220                 


Tyr His Ser Gly Trp Leu Pro Thr Ser Asn Phe Glu His Gly Phe Ser 
225                 230                 235                 240 


Arg Gly Ser His Pro Met Thr Tyr Phe Met Ser Leu Pro Val Gly Gly 
                245                 250                 255     


Asn Leu Pro Arg Ala Gln Phe Phe Gln Ser Ile Val Arg Ser Asn Ala 
            260                 265                 270         


Ile Asp Lys Gly Asp Gly Met Cys Thr Asn Phe Asp Val Asn Leu His 
        275                 280                 285             


Val Ala His Leu Ile Asn Arg Asp Leu Leu Val Ser Tyr Phe Asn Asn 
    290                 295                 300                 


Gly Ser Val Ala Asn Ala Ala Asp Cys Ala Asp Ser Ala Ala Glu Glu 
305                 310                 315                 320 


Leu Tyr Cys Val Thr Gly Ser Phe Asp Pro Pro Thr Gly Val Tyr Pro 
                325                 330                 335     


Leu Ser Arg Tyr Arg Ala Gln Val Ala Gly Phe Val Arg Val Thr Gln 
            340                 345                 350         


Arg Gly Ser Tyr Cys Thr Pro Pro Tyr Ser Val Leu Gln Asp Pro Pro 
        355                 360                 365             


Gln Pro Val Val Trp Arg Arg Tyr Met Leu Tyr Asp Cys Val Phe Asp 
    370                 375                 380                 


Phe Thr Val Val Val Asp Ser Leu Pro Thr His Gln Leu Gln Cys Tyr 
385                 390                 395                 400 


Gly Val Ser Pro Arg Arg Leu Ala Ser Met Cys Tyr Gly Ser Val Thr 
                405                 410                 415     


Leu Asp Val Met Arg Ile Asn Glu Thr His Leu Asn Asn Leu Phe Asn 
            420                 425                 430         


Arg Val Pro Asp Thr Phe Ser Leu Tyr Asn Tyr Ala Leu Pro Asp Asn 
        435                 440                 445             


Phe Tyr Gly Cys Leu His Ala Phe Tyr Leu Asn Ser Thr Ala Pro Tyr 
    450                 455                 460                 


Ala Val Ala Asn Arg Phe Pro Ile Lys Pro Gly Gly Arg Gln Ser Asn 
465                 470                 475                 480 


Ser Ala Phe Ile Asp Thr Val Ile Asn Ala Ala His Tyr Ser Pro Phe 
                485                 490                 495     


Ser Tyr Val Tyr Gly Leu Ala Val Ile Thr Leu Lys Pro Ala Ala Gly 
            500                 505                 510         


Ser Lys Leu Val Cys Pro Val Ala Asn Asp Thr Val Val Ile Thr Asp 
        515                 520                 525             


Arg Cys Val Gln Tyr Asn Leu Tyr Gly Tyr Thr Gly Thr Gly Val Leu 
    530                 535                 540                 


Ser Lys Asn Thr Ser Leu Val Ile Pro Asp Gly Lys Val Phe Thr Ala 
545                 550                 555                 560 


Ser Ser Thr Gly Thr Ile Ile Gly Val Ser Ile Asn Ser Thr Thr Tyr 
                565                 570                 575     


Ser Ile Met Pro Cys Val Thr Val Pro Val Ser Val Gly Tyr His Pro 
            580                 585                 590         


Asn Phe Glu Arg Ala Leu Leu Phe Asn Gly Leu Ser Cys Ser Gln Arg 
        595                 600                 605             


Ser Arg Ala Val Thr Glu Pro Val Ser Val Leu Trp Ser Ala Ser Ala 
    610                 615                 620                 


Thr Ala Gln Asp Ala Phe Asp Thr Pro Ser Gly Cys Val Val Asn Val 
625                 630                 635                 640 


Glu Leu Arg Asn Thr Thr Ile Val Asn Thr Cys Ala Met Pro Ile Gly 
                645                 650                 655     


Asn Ser Leu Cys Phe Ile Asn Gly Ser Ile Ala Thr Ala Asn Ala Asp 
            660                 665                 670         


Ser Leu Pro Arg Leu Gln Leu Val Asn Tyr Asp Pro Leu Tyr Asp Asn 
        675                 680                 685             


Ser Thr Ala Thr Pro Met Thr Pro Val Tyr Trp Val Lys Val Pro Thr 
    690                 695                 700                 


Asn Phe Thr Leu Ser Ala Thr Glu Glu Tyr Ile Gln Thr Thr Ala Pro 
705                 710                 715                 720 


Lys Ile Thr Ile Asp Cys Ala Arg Tyr Leu Cys Gly Asp Ser Ser Arg 
                725                 730                 735     


Cys Leu Asn Val Leu Leu His Tyr Gly Thr Phe Cys Asn Asp Ile Asn 
            740                 745                 750         


Lys Ala Leu Ser Arg Val Ser Thr Ile Leu Asp Ser Ala Leu Leu Ser 
        755                 760                 765             


Leu Val Lys Glu Leu Ser Ile Asn Thr Arg Asp Glu Val Thr Thr Phe 
    770                 775                 780                 


Ser Phe Asp Gly Asp Tyr Asn Phe Thr Gly Leu Met Gly Cys Leu Gly 
785                 790                 795                 800 


Pro Asn Cys Gly Ala Thr Thr Tyr Arg Ser Ala Phe Ser Asp Leu Leu 
                805                 810                 815     


Tyr Asp Lys Val Arg Ile Thr Asp Pro Gly Phe Met Gln Ser Tyr Gln 
            820                 825                 830         


Lys Cys Ile Asp Ser Gln Trp Gly Gly Ser Ile Arg Asp Leu Leu Cys 
        835                 840                 845             


Thr Gln Thr Tyr Asn Gly Ile Ala Val Leu Pro Pro Ile Val Ser Pro 
    850                 855                 860                 


Ala Met Gln Ala Leu Tyr Thr Ser Leu Leu Val Gly Ala Val Ala Ser 
865                 870                 875                 880 


Ser Gly Tyr Thr Phe Gly Ile Thr Ser Ala Gly Val Ile Pro Phe Ala 
                885                 890                 895     


Thr Gln Leu Gln Phe Arg Leu Asn Gly Ile Gly Val Thr Thr Gln Val 
            900                 905                 910         


Leu Val Glu Asn Gln Lys Leu Ile Ala Ser Ser Phe Asn Asn Ala Leu 
        915                 920                 925             


Val Asn Ile Gln Lys Gly Phe Thr Glu Thr Ser Ile Ala Leu Ser Lys 
    930                 935                 940                 


Met Gln Asp Val Ile Asn Gln His Ala Ala Gln Leu His Thr Leu Val 
945                 950                 955                 960 


Val Gln Leu Gly Asn Ser Phe Gly Ala Ile Ser Ser Ser Ile Asn Glu 
                965                 970                 975     


Ile Phe Ser Arg Leu Glu Pro Pro Ala Ala Asn Ala Glu Val Asp Arg 
            980                 985                 990         


Leu Ile Asn Gly Arg Met Met Val  Leu Asn Thr Tyr Val  Thr Gln Leu 
        995                 1000                 1005             


Leu Ile  Gln Ala Ser Glu Ala  Lys Ala Gln Asn Ala  Leu Ala Ala 
    1010                 1015                 1020             


Gln Lys  Ile Ser Glu Cys Val  Lys Ala Gln Ser Leu  Arg Asn Asp 
    1025                 1030                 1035             


Phe Cys  Gly Asn Gly Thr His  Val Leu Ser Ile Pro  Gln Leu Ala 
    1040                 1045                 1050             


Pro Asn  Gly Val Leu Phe Ile  His Tyr Ala Tyr Thr  Pro Thr Glu 
    1055                 1060                 1065             


Tyr Ala  Phe Val Gln Thr Ser  Ala Gly Leu Cys His  Asn Gly Thr 
    1070                 1075                 1080             


Gly Tyr  Ala Pro Arg Gln Gly  Met Phe Val Leu Pro  Asn Asn Thr 
    1085                 1090                 1095             


Asn Met  Trp His Phe Thr Thr  Met Gln Phe Tyr Asn  Pro Val Asn 
    1100                 1105                 1110             


Ile Ser  Ala Ser Asn Thr Gln  Val Leu Thr Ser Cys  Ser Val Asn 
    1115                 1120                 1125             


Tyr Thr  Ser Val Asn Tyr Thr  Val Leu Glu Pro Ser  Val Pro Gly 
    1130                 1135                 1140             


Asp Tyr  Asp Phe Gln Lys Glu  Phe Asp Lys Phe Tyr  Lys Asn Leu 
    1145                 1150                 1155             


Ser Thr  Ile Phe Asn Asn Thr  Phe Asn Pro Asn Asp  Phe Asn Phe 
    1160                 1165                 1170             


Ser Thr  Val Asp Val Thr Ala  Gln Ile Lys Ser Leu  His Asp Val 
    1175                 1180                 1185             


Val Asn  Gln Leu Asn Gln Ser  Phe Ile Asp Leu Lys  Lys Leu Asn 
    1190                 1195                 1200             


Val Tyr  Glu Lys Gly Gly Tyr  Ile Pro Glu Ala Pro  Arg Asp Gly 
    1205                 1210                 1215             


Gln Ala  Tyr Val Arg Lys Asp  Gly Glu Trp Val Leu  Leu Ser Thr 
    1220                 1225                 1230             


Phe 
    


<210>  33
<211>  1314
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  33

Met Phe Leu Ile Leu Leu Ile Ser Leu Pro Thr Ala Phe Ala Val Ile 
1               5                   10                  15      


Gly Asp Leu Lys Cys Pro Leu Asp Ser Arg Thr Gly Ser Leu Asn Asn 
            20                  25                  30          


Ile Asp Thr Gly Pro Pro Ser Ile Ser Thr Ala Thr Val Asp Val Thr 
        35                  40                  45              


Asn Gly Leu Gly Thr Tyr Tyr Val Leu Asp Arg Val Tyr Leu Asn Thr 
    50                  55                  60                  


Thr Leu Phe Leu Asn Gly Tyr Tyr Pro Thr Ser Gly Ser Thr Tyr Arg 
65                  70                  75                  80  


Asn Met Ala Leu Lys Gly Thr Asp Lys Leu Ser Thr Leu Trp Phe Lys 
                85                  90                  95      


Pro Pro Phe Leu Ser Asp Phe Ile Asn Gly Ile Phe Ala Lys Val Lys 
            100                 105                 110         


Asn Thr Lys Val Phe Lys Asp Gly Val Met Tyr Ser Glu Phe Pro Ala 
        115                 120                 125             


Ile Thr Ile Gly Ser Thr Phe Val Asn Thr Ser Tyr Ser Val Val Val 
    130                 135                 140                 


Gln Pro Arg Thr Ile Asn Ser Thr Gln Asp Gly Val Asn Lys Leu Gln 
145                 150                 155                 160 


Gly Leu Leu Glu Val Ser Val Cys Gln Tyr Asn Met Cys Glu Tyr Pro 
                165                 170                 175     


His Thr Ile Cys His Pro Lys Leu Gly Asn His Phe Lys Glu Leu Trp 
            180                 185                 190         


His Met Asp Thr Gly Val Val Ser Cys Leu Tyr Lys Arg Asn Phe Thr 
        195                 200                 205             


Tyr Asp Val Asn Ala Thr Tyr Leu Tyr Phe His Phe Tyr Gln Glu Gly 
    210                 215                 220                 


Gly Thr Phe Tyr Ala Tyr Phe Thr Asp Thr Gly Val Val Thr Lys Phe 
225                 230                 235                 240 


Leu Phe Asn Val Tyr Leu Gly Met Ala Leu Ser His Tyr Tyr Val Met 
                245                 250                 255     


Pro Leu Thr Cys Ile Ser Arg Arg Asp Ile Gly Phe Thr Leu Glu Tyr 
            260                 265                 270         


Trp Val Thr Pro Leu Thr Ser Arg Gln Tyr Leu Leu Ala Phe Asn Gln 
        275                 280                 285             


Asp Gly Ile Ile Phe Asn Ala Val Asp Cys Met Ser Asp Phe Met Ser 
    290                 295                 300                 


Glu Ile Lys Cys Lys Thr Gln Ser Ile Ala Pro Pro Thr Gly Val Tyr 
305                 310                 315                 320 


Glu Leu Asn Gly Tyr Thr Val Gln Pro Ile Ala Asp Val Tyr Arg Arg 
                325                 330                 335     


Lys Pro Asp Leu Pro Asn Cys Asn Ile Glu Ala Trp Leu Asn Asp Lys 
            340                 345                 350         


Ser Val Pro Ser Pro Leu Asn Trp Glu Arg Lys Thr Phe Ser Asn Cys 
        355                 360                 365             


Asn Phe Asn Met Ser Ser Leu Met Ser Phe Ile Gln Ala Asp Ser Phe 
    370                 375                 380                 


Thr Cys Asn Asn Ile Asp Ala Ala Lys Ile Tyr Gly Met Cys Phe Ser 
385                 390                 395                 400 


Ser Ile Thr Ile Asp Lys Phe Ala Ile Pro Asn Gly Arg Lys Val Asp 
                405                 410                 415     


Leu Gln Leu Gly Asn Leu Gly Tyr Leu Gln Ser Phe Asn Tyr Arg Ile 
            420                 425                 430         


Asp Thr Thr Ala Thr Ser Cys Gln Leu Tyr Tyr Asn Leu Pro Ala Ala 
        435                 440                 445             


Asn Val Ser Val Ser Arg Phe Asn Pro Ser Thr Trp Asn Lys Arg Phe 
    450                 455                 460                 


Gly Phe Ile Glu Asn Ser Val Phe Lys Pro Gln Pro Ala Gly Val Leu 
465                 470                 475                 480 


Thr Asn His Asp Val Val Tyr Ala Gln His Cys Phe Lys Ala Pro Lys 
                485                 490                 495     


Asn Phe Cys Pro Cys Lys Leu Asn Ser Ser Leu Cys Val Gly Ser Gly 
            500                 505                 510         


Pro Gly Lys Asn Asn Gly Ile Gly Thr Cys Pro Ala Gly Thr Asn Tyr 
        515                 520                 525             


Leu Thr Cys His Asn Leu Cys Asn Pro Asp Pro Ile Thr Phe Thr Gly 
    530                 535                 540                 


Pro Tyr Lys Cys Pro Gln Thr Lys Ser Leu Val Gly Ile Gly Glu His 
545                 550                 555                 560 


Cys Ser Gly Leu Ala Val Lys Ser Asp Tyr Cys Gly Gly Asn Pro Cys 
                565                 570                 575     


Thr Cys Gln Pro Gln Ala Phe Leu Gly Trp Ser Ala Asp Ser Cys Leu 
            580                 585                 590         


Gln Gly Asp Lys Cys Asn Ile Phe Ala Asn Leu Ile Leu His Asp Val 
        595                 600                 605             


Asn Ser Gly Leu Thr Cys Ser Thr Asp Leu Gln Lys Ala Asn Thr Asp 
    610                 615                 620                 


Ile Lys Leu Gly Val Cys Val Asn Tyr Asp Leu Tyr Gly Ile Ser Gly 
625                 630                 635                 640 


Gln Gly Ile Phe Val Glu Val Asn Ala Thr Tyr Tyr Asn Ser Trp Gln 
                645                 650                 655     


Asn Leu Leu Tyr Asp Ser Asn Gly Asn Leu Tyr Gly Phe Arg Asp Tyr 
            660                 665                 670         


Ile Thr Asn Arg Thr Phe Met Ile Arg Ser Cys Tyr Ser Gly Arg Val 
        675                 680                 685             


Ser Ala Ala Phe His Ala Asn Ser Ser Glu Pro Ala Leu Leu Phe Arg 
    690                 695                 700                 


Asn Ile Lys Cys Asn Tyr Val Phe Asn Asn Ser Leu Ile Arg Gln Leu 
705                 710                 715                 720 


Gln Pro Ile Asn Tyr Phe Asp Ser Tyr Leu Gly Cys Val Val Asn Ala 
                725                 730                 735     


Tyr Asn Ser Thr Ala Ile Ser Val Gln Thr Cys Asp Leu Thr Val Gly 
            740                 745                 750         


Ser Gly Tyr Cys Val Asp Tyr Ser Lys Asn Gly Gly Ser Gly Ser Ala 
        755                 760                 765             


Ile Thr Thr Gly Tyr Arg Phe Thr Asn Phe Glu Pro Phe Thr Val Asn 
    770                 775                 780                 


Ser Val Asn Asp Ser Leu Glu Pro Val Gly Gly Leu Tyr Glu Ile Gln 
785                 790                 795                 800 


Ile Pro Ser Glu Phe Thr Ile Gly Asn Met Glu Glu Phe Ile Gln Thr 
                805                 810                 815     


Ser Ser Pro Lys Val Thr Ile Asp Cys Ala Ala Phe Val Cys Gly Asp 
            820                 825                 830         


Tyr Ala Ala Cys Lys Ser Gln Leu Val Glu Tyr Gly Ser Phe Cys Asp 
        835                 840                 845             


Asn Ile Asn Ala Ile Leu Thr Glu Val Asn Glu Leu Leu Asp Thr Thr 
    850                 855                 860                 


Gln Leu Gln Val Ala Asn Ser Leu Met Asn Gly Val Thr Leu Ser Thr 
865                 870                 875                 880 


Lys Leu Lys Asp Gly Val Asn Phe Asn Val Asp Asp Ile Asn Phe Ser 
                885                 890                 895     


Ser Val Leu Gly Cys Leu Gly Ser Glu Cys Ser Lys Ala Ser Ser Arg 
            900                 905                 910         


Ser Ala Ile Glu Asp Leu Leu Phe Asp Lys Val Lys Leu Ser Asp Val 
        915                 920                 925             


Gly Phe Val Ala Ala Tyr Asn Asn Cys Thr Gly Gly Ala Glu Ile Arg 
    930                 935                 940                 


Asp Leu Ile Cys Val Gln Ser Tyr Lys Gly Ile Lys Val Leu Pro Pro 
945                 950                 955                 960 


Leu Leu Ser Glu Asn Gln Ile Ser Gly Tyr Thr Leu Ala Ala Thr Ser 
                965                 970                 975     


Ala Ser Leu Phe Pro Pro Trp Thr Ala Ala Ala Gly Val Pro Phe Tyr 
            980                 985                 990         


Leu Asn Val Gln Tyr Arg Ile Asn  Gly Leu Gly Val Thr  Met Asp Val 
        995                 1000                 1005             


Leu Ser  Gln Asn Gln Lys Leu  Ile Ala Asn Ala Phe  Asn Asn Ala 
    1010                 1015                 1020             


Leu Asp  Ala Ile Gln Glu Gly  Phe Asp Ala Thr Asn  Ser Ala Leu 
    1025                 1030                 1035             


Val Lys  Ile Gln Ala Val Val  Asn Ala Asn Ala Glu  Ala Leu Asn 
    1040                 1045                 1050             


Asn Leu  Leu Gln Gln Leu Ser  Asn Arg Phe Gly Ala  Ile Ser Ser 
    1055                 1060                 1065             


Ser Leu  Gln Glu Ile Leu Ser  Arg Leu Asp Pro Pro  Glu Ala Glu 
    1070                 1075                 1080             


Ala Gln  Ile Asp Arg Leu Ile  Asn Gly Arg Leu Thr  Ala Leu Asn 
    1085                 1090                 1095             


Ala Tyr  Val Ser Gln Gln Leu  Ser Asp Ser Thr Leu  Val Lys Phe 
    1100                 1105                 1110             


Ser Ala  Ala Gln Ala Met Glu  Lys Val Asn Glu Cys  Val Lys Ser 
    1115                 1120                 1125             


Gln Ser  Ser Arg Ile Asn Phe  Cys Gly Asn Gly Asn  His Ile Ile 
    1130                 1135                 1140             


Ser Leu  Val Gln Asn Ala Pro  Tyr Gly Leu Tyr Phe  Ile His Phe 
    1145                 1150                 1155             


Ser Tyr  Val Pro Thr Lys Tyr  Val Thr Ala Lys Val  Ser Pro Gly 
    1160                 1165                 1170             


Leu Cys  Ile Ala Gly Asp Arg  Gly Ile Ala Pro Lys  Ser Gly Tyr 
    1175                 1180                 1185             


Phe Val  Asn Val Asn Asn Thr  Trp Met Tyr Thr Gly  Ser Gly Tyr 
    1190                 1195                 1200             


Tyr Tyr  Pro Glu Pro Ile Thr  Glu Asn Asn Val Val  Val Met Ser 
    1205                 1210                 1215             


Thr Cys  Ala Val Asn Tyr Thr  Lys Ala Pro Tyr Val  Met Leu Asn 
    1220                 1225                 1230             


Thr Ser  Thr Pro Asn Leu Pro  Asp Phe Arg Glu Glu  Leu Asp Gln 
    1235                 1240                 1245             


Trp Phe  Lys Asn Gln Thr Ser  Val Ala Pro Asp Leu  Ser Leu Asp 
    1250                 1255                 1260             


Tyr Ile  Asn Val Thr Phe Leu  Asp Leu Gln Val Glu  Met Asn Arg 
    1265                 1270                 1275             


Leu Gln  Glu Ala Ile Lys Val  Leu Asn Gly Gly Tyr  Ile Pro Glu 
    1280                 1285                 1290             


Ala Pro  Arg Asp Gly Gln Ala  Tyr Val Arg Lys Asp  Gly Glu Trp 
    1295                 1300                 1305             


Val Leu  Leu Ser Thr Phe 
    1310                 


<210>  34
<211>  1218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  34

Met Lys Leu Leu Val Leu Val Phe Ala Thr Leu Val Ser Ser Tyr Thr 
1               5                   10                  15      


Ile Glu Lys Cys Leu Asp Phe Asp Asp Arg Thr Pro Pro Ala Asn Thr 
            20                  25                  30          


Gln Phe Leu Ser Ser His Arg Gly Val Tyr Tyr Pro Asp Asp Ile Phe 
        35                  40                  45              


Arg Ser Asn Val Leu His Leu Val Gln Asp His Phe Leu Pro Phe Asp 
    50                  55                  60                  


Ser Asn Val Thr Arg Phe Ile Thr Phe Gly Leu Asn Phe Asp Asn Pro 
65                  70                  75                  80  


Ile Ile Pro Phe Lys Asp Gly Ile Tyr Phe Ala Ala Thr Glu Lys Ser 
                85                  90                  95      


Asn Val Ile Arg Gly Trp Val Phe Gly Ser Thr Met Asn Asn Lys Ser 
            100                 105                 110         


Gln Ser Val Ile Ile Met Asn Asn Ser Thr Asn Leu Val Ile Arg Ala 
        115                 120                 125             


Cys Asn Phe Glu Leu Cys Asp Asn Pro Phe Phe Val Val Leu Lys Ser 
    130                 135                 140                 


Asn Asn Thr Gln Ile Pro Ser Tyr Ile Phe Asn Asn Ala Phe Asn Cys 
145                 150                 155                 160 


Thr Phe Glu Tyr Val Ser Lys Asp Phe Asn Leu Asp Leu Gly Glu Lys 
                165                 170                 175     


Pro Gly Asn Phe Lys Asp Leu Arg Glu Phe Val Phe Arg Asn Lys Asp 
            180                 185                 190         


Gly Phe Leu His Val Tyr Ser Gly Tyr Gln Pro Ile Ser Ala Ala Ser 
        195                 200                 205             


Gly Leu Pro Thr Gly Phe Asn Ala Leu Lys Pro Ile Phe Lys Leu Pro 
    210                 215                 220                 


Leu Gly Ile Asn Ile Thr Asn Phe Arg Thr Leu Leu Thr Ala Phe Pro 
225                 230                 235                 240 


Pro Arg Pro Asp Tyr Trp Gly Thr Ser Ala Ala Ala Tyr Phe Val Gly 
                245                 250                 255     


Tyr Leu Lys Pro Thr Thr Phe Met Leu Lys Tyr Asp Glu Asn Gly Thr 
            260                 265                 270         


Ile Thr Asp Ala Val Asp Cys Ser Gln Asn Pro Leu Ala Glu Leu Lys 
        275                 280                 285             


Cys Ser Val Lys Ser Phe Glu Ile Asp Lys Gly Ile Tyr Gln Thr Ser 
    290                 295                 300                 


Asn Phe Arg Val Ala Pro Ser Lys Glu Val Val Arg Phe Pro Asn Ile 
305                 310                 315                 320 


Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Thr Phe Pro 
                325                 330                 335     


Ser Val Tyr Ala Trp Glu Arg Lys Arg Ile Ser Asn Cys Val Ala Asp 
            340                 345                 350         


Tyr Ser Val Leu Tyr Asn Ser Thr Ser Phe Ser Thr Phe Lys Cys Tyr 
        355                 360                 365             


Gly Val Ser Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr 
    370                 375                 380                 


Ala Asp Ser Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro 
385                 390                 395                 400 


Gly Gln Thr Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp 
                405                 410                 415     


Phe Thr Gly Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr 
            420                 425                 430         


Gln Thr Gly Asn Tyr Asn Tyr Lys Tyr Arg Ser Leu Arg His Gly Lys 
        435                 440                 445             


Leu Arg Pro Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp 
    450                 455                 460                 


Gly Lys Pro Cys Thr Pro Pro Ala Phe Asn Cys Tyr Trp Pro Leu Asn 
465                 470                 475                 480 


Asp Tyr Gly Phe Tyr Ile Thr Asn Gly Ile Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Leu Ser Thr Asp Leu Ile Lys Asn Gln Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Pro Ser Ser Lys 
    530                 535                 540                 


Arg Phe Gln Pro Phe Gln Gln Phe Gly Arg Asp Val Ser Asp Phe Thr 
545                 550                 555                 560 


Asp Ser Val Arg Asp Pro Lys Thr Ser Glu Ile Leu Asp Ile Ser Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Ser Glu Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Asp Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Ser Trp Arg Val His Ser 
    610                 615                 620                 


Thr Gly Asn Asn Val Phe Gln Thr Gln Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asp Thr Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr His Thr Val Ser Ser Leu Arg Ser Thr Ser Gln 
            660                 665                 670         


Lys Ser Ile Val Ala Tyr Thr Met Ser Leu Gly Ala Asp Ser Ser Ile 
        675                 680                 685             


Ala Tyr Ser Asn Asn Thr Ile Ala Ile Pro Thr Asn Phe Ser Ile Ser 
    690                 695                 700                 


Ile Thr Thr Glu Val Met Pro Val Ser Met Ala Lys Thr Ser Val Asp 
705                 710                 715                 720 


Cys Asn Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ala Asn Leu Leu 
                725                 730                 735     


Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Ser Gly 
            740                 745                 750         


Ile Ala Val Glu Gln Asp Arg Asn Thr Arg Glu Val Phe Ala Gln Val 
        755                 760                 765             


Lys Gln Met Tyr Lys Thr Pro Thr Leu Lys Asp Phe Gly Gly Phe Asn 
    770                 775                 780                 


Phe Ser Gln Ile Leu Pro Asp Pro Leu Lys Pro Thr Lys Arg Ser Phe 
785                 790                 795                 800 


Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe 
                805                 810                 815     


Met Lys Gln Tyr Gly Glu Cys Leu Gly Asp Ile Asn Ala Arg Asp Leu 
            820                 825                 830         


Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu 
        835                 840                 845             


Thr Asp Asp Met Ile Ala Ala Tyr Thr Ala Ala Leu Val Ser Gly Thr 
    850                 855                 860                 


Ala Thr Ala Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro 
865                 870                 875                 880 


Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln 
                885                 890                 895     


Asn Val Leu Tyr Glu Asn Gln Lys Gln Ile Ala Asn Gln Phe Asn Lys 
            900                 905                 910         


Ala Ile Ser Gln Ile Gln Glu Ser Leu Thr Thr Thr Ser Thr Ala Leu 
        915                 920                 925             


Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr 
    930                 935                 940                 


Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu 
945                 950                 955                 960 


Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile 
                965                 970                 975     


Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr 
            980                 985                 990         


Gln Gln Leu Ile Arg Ala Ala Glu  Ile Arg Ala Ser Ala  Asn Leu Ala 
        995                 1000                 1005             


Ala Thr  Lys Met Ser Glu Cys  Val Leu Gly Gln Ser  Lys Arg Val 
    1010                 1015                 1020             


Asp Phe  Cys Gly Lys Gly Tyr  His Leu Met Ser Phe  Pro Gln Ala 
    1025                 1030                 1035             


Ala Pro  His Gly Val Val Phe  Leu His Val Thr Tyr  Val Pro Ser 
    1040                 1045                 1050             


Gln Glu  Arg Asn Phe Thr Thr  Ala Pro Ala Ile Cys  His Glu Gly 
    1055                 1060                 1065             


Lys Ala  Tyr Phe Pro Arg Glu  Gly Val Phe Val Phe  Asn Gly Thr 
    1070                 1075                 1080             


Ser Trp  Phe Ile Thr Gln Arg  Asn Phe Phe Ser Pro  Gln Ile Ile 
    1085                 1090                 1095             


Thr Thr  Asp Asn Thr Phe Val  Ser Gly Ser Cys Asp  Val Val Ile 
    1100                 1105                 1110             


Gly Ile  Ile Asn Asn Thr Val  Tyr Asp Pro Leu Gln  Pro Glu Leu 
    1115                 1120                 1125             


Asp Ser  Phe Lys Glu Glu Leu  Asp Lys Tyr Phe Lys  Asn His Thr 
    1130                 1135                 1140             


Ser Pro  Asp Val Asp Leu Gly  Asp Ile Ser Gly Ile  Asn Ala Ser 
    1145                 1150                 1155             


Val Val  Asn Ile Gln Lys Glu  Ile Asp Arg Leu Asn  Glu Val Ala 
    1160                 1165                 1170             


Lys Asn  Leu Asn Glu Ser Leu  Ile Asp Leu Gln Glu  Leu Gly Lys 
    1175                 1180                 1185             


Tyr Glu  Gln Gly Gly Tyr Ile  Pro Glu Ala Pro Arg  Asp Gly Gln 
    1190                 1195                 1200             


Ala Tyr  Val Arg Lys Asp Gly  Glu Trp Val Leu Leu  Ser Thr Phe 
    1205                 1210                 1215             


<210>  35
<211>  1324
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  35

Met Leu Ser Val Phe Ile Leu Phe Leu Pro Ser Cys Leu Gly Tyr Ile 
1               5                   10                  15      


Gly Asp Phe Arg Cys Ile Asn Leu Val Asn Thr Asp Thr Ser Asn Ala 
            20                  25                  30          


Ser Ala Pro Ser Val Ser Thr Glu Val Val Asp Val Ser Lys Gly Ile 
        35                  40                  45              


Gly Thr Tyr Tyr Val Leu Asp Arg Val Tyr Leu Asn Ala Thr Leu Leu 
    50                  55                  60                  


Leu Thr Gly Tyr Tyr Pro Val Asp Gly Ser Asn Tyr Arg Asn Leu Ala 
65                  70                  75                  80  


Leu Thr Gly Thr Asn Thr Leu Ser Leu Asn Trp Tyr Lys Pro Pro Phe 
                85                  90                  95      


Leu Ser Glu Phe Asn Asp Gly Ile Phe Ala Lys Val Lys Asn Leu Lys 
            100                 105                 110         


Ala Ser Leu Pro Lys Asp Ser Thr Ser Tyr Phe Pro Thr Ile Val Ile 
        115                 120                 125             


Gly Ser Asn Phe Val Thr Thr Ser Tyr Thr Val Val Leu Glu Pro Tyr 
    130                 135                 140                 


Asn Gly Ile Ile Met Ala Ser Ile Cys Gln Tyr Thr Ile Cys Leu Leu 
145                 150                 155                 160 


Pro Tyr Thr Asp Cys Lys Pro Asn Thr Gly Gly Asn Lys Leu Ile Gly 
                165                 170                 175     


Phe Trp His Ile Asp Leu Lys Ser Pro Val Cys Ile Leu Lys Arg Asn 
            180                 185                 190         


Phe Thr Phe Asn Val Asn Ala Asp Trp Leu Tyr Phe His Phe Tyr Gln 
        195                 200                 205             


Gln Gly Gly Thr Phe Tyr Ala Tyr Tyr Ala Asp Ala Gly Ser Ala Thr 
    210                 215                 220                 


Thr Phe Leu Phe Ser Ser Tyr Ile Gly Asp Val Leu Thr Gln Tyr Phe 
225                 230                 235                 240 


Val Leu Pro Phe Val Cys Thr Pro Thr Thr Thr Gly Val Phe Ser Pro 
                245                 250                 255     


Gln Tyr Trp Val Thr Pro Leu Val Lys Arg Gln Tyr Leu Phe Asn Phe 
            260                 265                 270         


Asn Gln Lys Gly Thr Ile Thr Ser Ala Val Asp Cys Ala Ser Ser Tyr 
        275                 280                 285             


Thr Ser Glu Ile Lys Cys Lys Thr Gln Ser Met Asn Pro Asn Thr Gly 
    290                 295                 300                 


Val Tyr Asp Leu Ser Gly Tyr Thr Val Gln Pro Val Gly Leu Val Tyr 
305                 310                 315                 320 


Arg Arg Val Arg Asn Leu Pro Asp Cys Arg Ile Glu Asp Trp Leu Ala 
                325                 330                 335     


Ala Lys Thr Val Pro Ser Pro Leu Asn Trp Glu Arg Lys Thr Phe Gln 
            340                 345                 350         


Asn Cys Asn Phe Asn Leu Ser Ser Leu Leu Arg Leu Val Gln Ala Gly 
        355                 360                 365             


Ser Leu Ser Cys Ser Asn Ile Asp Ala Ala Lys Val Tyr Gly Met Cys 
    370                 375                 380                 


Phe Gly Ser Met Ser Ile Asp Lys Phe Ala Ile Pro Asn Ser Arg Arg 
385                 390                 395                 400 


Val Asp Leu Gln Leu Gly Asn Ser Gly Phe Leu Gln Ser Phe Asn Tyr 
                405                 410                 415     


Lys Ile Asp Thr Arg Ala Thr Ser Cys Gln Leu Tyr Tyr Ser Leu Ala 
            420                 425                 430         


Gln Ser Asn Val Thr Val Asn Asn His Asn Pro Ser Ser Trp Asn Arg 
        435                 440                 445             


Arg Tyr Gly Phe Asn Asp Val Ala Thr Phe Gly Arg Gly Lys His Asp 
    450                 455                 460                 


Val Ala Tyr Ala Glu Ala Cys Phe Thr Val Gly Ala Ser Tyr Cys Pro 
465                 470                 475                 480 


Cys Ala Asn Pro Ser Ile Val Ser Pro Cys Thr Thr Gly Lys Pro Lys 
                485                 490                 495     


Phe Ala Asn Cys Pro Thr Gly Thr Thr Asn Arg Glu Cys Asn Val Leu 
            500                 505                 510         


Ala Leu Gly Ser Asn Leu Phe Lys Cys Asp Cys Thr Cys Asn Pro Ser 
        515                 520                 525             


Pro Leu Thr Thr Tyr Asp Leu Arg Cys Leu Gln Gly Arg Ser Met Leu 
    530                 535                 540                 


Gly Val Gly Asp His Cys Glu Gly Leu Gly Val Leu Glu Asp Lys Cys 
545                 550                 555                 560 


Gly Gly Ser Asn Thr Cys Asn Cys Ser Ala Asp Ala Phe Val Gly Trp 
                565                 570                 575     


Ala Lys Asp Ser Cys Leu Ser Asn Gly Arg Cys His Ile Phe Ser Asn 
            580                 585                 590         


Leu Met Leu Asn Gly Ile Asn Ser Gly Thr Thr Cys Ser Thr Asp Leu 
        595                 600                 605             


Gln Leu Pro Asn Thr Glu Val Val Thr Gly Ile Cys Val Lys Tyr Asp 
    610                 615                 620                 


Leu Tyr Gly Ile Thr Gly Gln Gly Val Phe Lys Glu Val Lys Ala Asp 
625                 630                 635                 640 


Tyr Tyr Asn Ser Trp Gln Asn Leu Leu Tyr Asp Val Asn Gly Asn Leu 
                645                 650                 655     


Asn Gly Phe Arg Asp Ile Val Thr Asn Lys Thr Tyr Leu Thr Arg Ser 
            660                 665                 670         


Cys Tyr Ser Gly Arg Val Ser Ala Ala Tyr His Gln Asp Ala Pro Glu 
        675                 680                 685             


Pro Ala Leu Leu Tyr Arg Asn Leu Lys Cys Asp Tyr Val Phe Asn Asn 
    690                 695                 700                 


Asn Ile Phe Arg Glu Glu Thr Pro Leu Asn Tyr Phe Asp Ser Tyr Leu 
705                 710                 715                 720 


Gly Cys Val Val Asn Ala Asp Asn Ser Thr Glu Gln Ala Val Asp Ala 
                725                 730                 735     


Cys Asp Leu Arg Met Gly Ser Gly Leu Cys Val Asn Tyr Ser Thr Ala 
            740                 745                 750         


His Arg Ala Arg Thr Ser Val Ser Thr Gly Tyr Lys Leu Thr Thr Phe 
        755                 760                 765             


Glu Pro Phe Thr Val Ser Ile Val Asn Asp Ser Val Glu Ser Val Gly 
    770                 775                 780                 


Gly Leu Tyr Glu Met Gln Ile Pro Thr Asn Phe Thr Ile Ala Ser His 
785                 790                 795                 800 


Gln Glu Phe Ile Gln Thr Arg Ala Pro Lys Val Thr Ile Asp Cys Ala 
                805                 810                 815     


Ala Phe Val Cys Gly Asp Tyr Thr Thr Cys Arg Gln Gln Leu Val Glu 
            820                 825                 830         


Tyr Gly Ser Phe Cys Asp Asn Ile Asn Ala Ile Leu Gly Glu Val Asn 
        835                 840                 845             


Asn Leu Ile Asp Thr Met Gln Leu Gln Val Ala Ser Ala Leu Ile Gln 
    850                 855                 860                 


Gly Val Thr Leu Ser Ser Arg Leu Ala Asp Gly Ile Ser Gly Gln Ile 
865                 870                 875                 880 


Asp Asp Ile Asn Phe Ser Pro Leu Leu Gly Cys Leu Gly Ser Gln Cys 
                885                 890                 895     


Ser Glu Gly Thr Met Ala Ala Gln Gly Arg Ser Thr Val Glu Asp Leu 
            900                 905                 910         


Leu Phe Asp Lys Val Lys Leu Ser Asp Val Gly Phe Val Glu Ala Tyr 
        915                 920                 925             


Asn Asn Cys Thr Gly Gly Gln Glu Val Arg Asp Leu Leu Cys Val Gln 
    930                 935                 940                 


Ser Phe Asn Gly Ile Lys Val Leu Pro Pro Val Leu Ser Glu Asn Gln 
945                 950                 955                 960 


Val Ser Gly Tyr Thr Ala Gly Ala Thr Ala Ser Ser Met Phe Pro Pro 
                965                 970                 975     


Trp Ser Ala Ala Ala Gly Val Pro Phe Ser Leu Ser Val Gln Tyr Arg 
            980                 985                 990         


Ile Asn Gly Leu Gly Val Thr Met  Asn Val Leu Ser Glu  Asn Gln Lys 
        995                 1000                 1005             


Met Ile  Ala Ser Ala Phe Asn  Asn Ala Ile Gly Ala  Ile Gln Glu 
    1010                 1015                 1020             


Gly Phe  Asp Ala Thr Asn Ser  Ala Leu Ala Lys Ile  Gln Ser Val 
    1025                 1030                 1035             


Val Asn  Ala Asn Ala Glu Ala  Leu Asn Asn Leu Leu  Asn Gln Leu 
    1040                 1045                 1050             


Ser Asn  Arg Phe Gly Ala Ile  Ser Ala Ser Leu Gln  Glu Ile Leu 
    1055                 1060                 1065             


Ser Arg  Leu Asp Pro Pro Glu  Ala Gln Ala Gln Ile  Asp Arg Leu 
    1070                 1075                 1080             


Ile Asn  Gly Arg Leu Thr Ala  Leu Asn Ala Tyr Val  Ser Lys Gln 
    1085                 1090                 1095             


Leu Ser  Asp Met Thr Leu Ile  Lys Val Ser Ala Ala  Gln Ala Ile 
    1100                 1105                 1110             


Glu Lys  Val Asn Glu Cys Val  Lys Ser Gln Ser Pro  Arg Ile Asn 
    1115                 1120                 1125             


Phe Cys  Gly Asn Gly Asn His  Ile Leu Ser Leu Val  Gln Asn Ala 
    1130                 1135                 1140             


Pro Tyr  Gly Leu Tyr Phe Leu  His Phe Ser Tyr Val  Pro Thr Ser 
    1145                 1150                 1155             


Phe Thr  Thr Ala Asn Val Ser  Pro Gly Leu Cys Ile  Ser Gly Asp 
    1160                 1165                 1170             


Arg Gly  Leu Ala Pro Lys Ala  Gly Tyr Phe Val Gln  Asp Asp Gly 
    1175                 1180                 1185             


Glu Trp  Lys Phe Thr Gly Ser  Asn Tyr Tyr Tyr Pro  Glu Pro Ile 
    1190                 1195                 1200             


Thr Asp  Lys Asn Ser Val Val  Met Ser Ser Cys Ala  Val Asn Tyr 
    1205                 1210                 1215             


Thr Lys  Ala Pro Glu Val Phe  Leu Asn Thr Ser Ile  Ser Asn Leu 
    1220                 1225                 1230             


Pro Asp  Phe Lys Glu Glu Leu  Asp Lys Trp Phe Lys  Asn Gln Thr 
    1235                 1240                 1245             


Ser Val  Ala Pro Asp Leu Ser  Leu Asp Phe Glu Lys  Leu Asn Val 
    1250                 1255                 1260             


Thr Phe  Leu Asp Leu Ser Asp  Glu Met Asn Arg Ile  Gln Glu Ala 
    1265                 1270                 1275             


Ile Lys  Lys Leu Asn Glu Ser  Tyr Ile Asn Leu Lys  Glu Ile Gly 
    1280                 1285                 1290             


Thr Tyr  Glu Met Gly Gly Tyr  Ile Pro Glu Ala Pro  Arg Asp Gly 
    1295                 1300                 1305             


Gln Ala  Tyr Val Arg Lys Asp  Gly Glu Trp Val Leu  Leu Ser Thr 
    1310                 1315                 1320             


Phe 
    


<210>  36
<211>  1318
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  36

Met Lys Leu Phe Leu Ile Leu Leu Val Leu Pro Leu Ala Ser Cys Phe 
1               5                   10                  15      


Phe Thr Cys Asn Ser Asn Ala Asn Leu Ser Met Leu Gln Leu Gly Val 
            20                  25                  30          


Pro Asp Asn Ser Ser Thr Ile Val Thr Gly Leu Leu Pro Thr His Trp 
        35                  40                  45              


Phe Cys Ala Asn Gln Ser Thr Ser Val Tyr Ser Ala Asn Gly Phe Phe 
    50                  55                  60                  


Tyr Ile Asp Val Gly Asn His Arg Ser Ala Phe Ala Leu His Thr Gly 
65                  70                  75                  80  


Tyr Tyr Asp Ala Asn Gln Tyr Tyr Ile Tyr Val Thr Asn Glu Ile Gly 
                85                  90                  95      


Leu Asn Ala Ser Val Thr Leu Lys Ile Cys Lys Phe Ser Arg Asn Thr 
            100                 105                 110         


Thr Phe Asp Phe Leu Ser Asn Ala Ser Ser Ser Phe Asp Cys Ile Val 
        115                 120                 125             


Asn Leu Leu Phe Thr Glu Gln Leu Gly Ala Pro Leu Gly Ile Thr Ile 
    130                 135                 140                 


Ser Gly Glu Thr Val Arg Leu His Leu Tyr Asn Val Thr Arg Thr Phe 
145                 150                 155                 160 


Tyr Val Pro Ala Ala Tyr Lys Leu Thr Lys Leu Ser Val Lys Cys Tyr 
                165                 170                 175     


Phe Asn Tyr Ser Cys Val Phe Ser Val Val Asn Ala Thr Val Thr Val 
            180                 185                 190         


Asn Val Thr Thr His Asn Gly Arg Val Val Asn Tyr Thr Val Cys Asp 
        195                 200                 205             


Asp Cys Asn Gly Tyr Thr Asp Asn Ile Phe Ser Val Gln Gln Asp Gly 
    210                 215                 220                 


Arg Ile Pro Asn Gly Phe Pro Phe Asn Asn Trp Phe Leu Leu Thr Asn 
225                 230                 235                 240 


Gly Ser Thr Leu Val Asp Gly Val Ser Arg Leu Tyr Gln Pro Leu Arg 
                245                 250                 255     


Leu Thr Cys Leu Trp Pro Val Pro Gly Leu Lys Ser Ser Thr Gly Phe 
            260                 265                 270         


Val Tyr Phe Asn Ala Thr Gly Ser Asp Val Asn Cys Asn Gly Tyr Gln 
        275                 280                 285             


His Asn Ser Val Val Asp Val Met Arg Tyr Asn Leu Asn Phe Ser Ala 
    290                 295                 300                 


Asn Ser Leu Asp Asn Leu Lys Ser Gly Val Ile Val Phe Lys Thr Leu 
305                 310                 315                 320 


Gln Tyr Asp Val Leu Phe Tyr Cys Ser Asn Ser Ser Ser Gly Val Leu 
                325                 330                 335     


Asp Thr Thr Ile Pro Phe Gly Pro Ser Ser Gln Pro Tyr Tyr Cys Phe 
            340                 345                 350         


Ile Asn Ser Thr Ile Asn Thr Thr His Val Ser Thr Phe Val Gly Ile 
        355                 360                 365             


Leu Pro Pro Thr Val Arg Glu Ile Val Val Ala Arg Thr Gly Gln Phe 
    370                 375                 380                 


Tyr Ile Asn Gly Phe Lys Tyr Phe Asp Leu Gly Phe Ile Glu Ala Val 
385                 390                 395                 400 


Asn Phe Asn Val Thr Thr Ala Ser Ala Thr Asp Phe Trp Thr Val Ala 
                405                 410                 415     


Phe Ala Thr Phe Val Asp Val Leu Val Asn Val Ser Ala Thr Asn Ile 
            420                 425                 430         


Gln Asn Leu Leu Tyr Cys Asp Ser Pro Phe Glu Lys Leu Gln Cys Glu 
        435                 440                 445             


His Leu Gln Phe Gly Leu Gln Asp Gly Phe Tyr Ser Ala Asn Phe Leu 
    450                 455                 460                 


Asp Asp Asn Val Leu Pro Glu Thr Tyr Val Ala Leu Pro Ile Tyr Tyr 
465                 470                 475                 480 


Gln His Thr Asp Ile Asn Phe Thr Ala Thr Ala Ser Phe Gly Gly Ser 
                485                 490                 495     


Cys Tyr Val Cys Lys Pro His Gln Val Asn Ile Ser Leu Asn Gly Asn 
            500                 505                 510         


Thr Ser Val Cys Val Arg Thr Ser His Phe Ser Ile Arg Tyr Ile Tyr 
        515                 520                 525             


Asn Arg Val Lys Ser Gly Ser Pro Gly Asp Ser Ser Trp His Ile Tyr 
    530                 535                 540                 


Leu Lys Ser Gly Thr Cys Pro Phe Ser Phe Ser Lys Leu Asn Asn Phe 
545                 550                 555                 560 


Gln Lys Phe Lys Thr Ile Cys Phe Ser Thr Val Glu Val Pro Gly Ser 
                565                 570                 575     


Cys Asn Phe Pro Leu Glu Ala Thr Trp His Tyr Thr Ser Tyr Thr Ile 
            580                 585                 590         


Val Gly Ala Leu Tyr Val Thr Trp Ser Glu Gly Asn Ser Ile Thr Gly 
        595                 600                 605             


Val Pro Tyr Pro Val Ser Gly Ile Arg Glu Phe Ser Asn Leu Val Leu 
    610                 615                 620                 


Asn Asn Cys Thr Lys Tyr Asn Ile Tyr Asp Tyr Val Gly Thr Gly Ile 
625                 630                 635                 640 


Ile Arg Ser Ser Asn Gln Ser Leu Ala Gly Gly Ile Thr Tyr Val Ser 
                645                 650                 655     


Asn Ser Gly Asn Leu Leu Gly Phe Lys Asn Val Ser Thr Gly Asn Ile 
            660                 665                 670         


Phe Ile Val Thr Pro Cys Asn Gln Pro Asp Gln Val Ala Val Tyr Gln 
        675                 680                 685             


Gln Ser Ile Ile Gly Ala Met Thr Ala Val Asn Glu Ser Arg Tyr Gly 
    690                 695                 700                 


Leu Gln Asn Leu Leu Gln Leu Pro Asn Phe Tyr Tyr Val Ser Asn Gly 
705                 710                 715                 720 


Gly Asn Asn Cys Thr Thr Ala Val Met Thr Tyr Ser Asn Phe Gly Ile 
                725                 730                 735     


Cys Ala Asp Gly Ser Leu Ile Pro Val Arg Pro Arg Asn Ser Ser Asp 
            740                 745                 750         


Asn Gly Ile Ser Ala Ile Ile Thr Ala Asn Leu Ser Ile Pro Ser Asn 
        755                 760                 765             


Trp Thr Thr Ser Val Gln Val Glu Tyr Leu Gln Ile Thr Ser Thr Pro 
    770                 775                 780                 


Ile Val Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn Pro Arg Cys 
785                 790                 795                 800 


Lys Asn Leu Leu Lys Gln Tyr Thr Ser Ala Cys Lys Thr Ile Glu Asp 
                805                 810                 815     


Ala Leu Arg Leu Ser Ala His Leu Glu Thr Asn Asp Val Ser Ser Met 
            820                 825                 830         


Leu Thr Phe Asp Ser Asn Ala Phe Ser Leu Ala Asn Val Thr Ser Phe 
        835                 840                 845             


Gly Asp Tyr Asn Leu Ser Ser Val Leu Pro Gln Arg Asn Ile Arg Ser 
    850                 855                 860                 


Ser Arg Ile Ala Gly Arg Ser Ala Leu Glu Asp Leu Leu Phe Ser Lys 
865                 870                 875                 880 


Val Val Thr Ser Gly Leu Gly Thr Val Asp Val Asp Tyr Lys Ser Cys 
                885                 890                 895     


Thr Lys Gly Leu Ser Ile Ala Asp Leu Ala Cys Ala Gln Tyr Tyr Asn 
            900                 905                 910         


Gly Ile Met Val Leu Pro Gly Val Ala Asp Ala Glu Arg Met Ala Met 
        915                 920                 925             


Tyr Thr Gly Ser Leu Ile Gly Gly Met Val Leu Gly Gly Leu Thr Ser 
    930                 935                 940                 


Ala Ala Ala Ile Pro Phe Ser Leu Ala Leu Gln Ala Arg Leu Asn Tyr 
945                 950                 955                 960 


Val Ala Leu Gln Thr Asp Val Leu Gln Glu Asn Gln Lys Ile Leu Ala 
                965                 970                 975     


Ala Ser Phe Asn Lys Ala Ile Asn Asn Ile Val Ala Ser Phe Ser Ser 
            980                 985                 990         


Val Asn Asp Ala Ile Thr Gln Thr  Ala Glu Ala Ile His  Thr Val Thr 
        995                 1000                 1005             


Ile Ala  Leu Asn Lys Ile Gln  Asp Val Val Asn Gln  Gln Gly Ser 
    1010                 1015                 1020             


Ala Leu  Asn His Leu Thr Ser  Gln Leu Arg His Asn  Phe Gln Ala 
    1025                 1030                 1035             


Ile Ser  Asn Ser Ile Gln Ala  Ile Tyr Asp Arg Leu  Asp Pro Pro 
    1040                 1045                 1050             


Gln Ala  Asp Gln Gln Val Asp  Arg Leu Ile Thr Gly  Arg Leu Ala 
    1055                 1060                 1065             


Ala Leu  Asn Ala Phe Val Ser  Gln Val Leu Asn Lys  Tyr Thr Glu 
    1070                 1075                 1080             


Val Arg  Gly Ser Arg Arg Leu  Ala Gln Gln Lys Ile  Asn Glu Cys 
    1085                 1090                 1095             


Val Lys  Ser Gln Ser Asn Arg  Tyr Gly Phe Cys Gly  Asn Gly Thr 
    1100                 1105                 1110             


His Ile  Phe Ser Ile Val Asn  Ser Ala Pro Asp Gly  Leu Leu Phe 
    1115                 1120                 1125             


Leu His  Thr Val Leu Leu Pro  Thr Asp Tyr Lys Asn  Val Lys Ala 
    1130                 1135                 1140             


Trp Ser  Gly Ile Cys Val Asp  Gly Ile Tyr Gly Tyr  Val Leu Arg 
    1145                 1150                 1155             


Gln Pro  Asn Leu Val Leu Tyr  Ser Asp Asn Gly Val  Phe Arg Val 
    1160                 1165                 1170             


Thr Ser  Arg Ile Met Phe Gln  Pro Arg Leu Pro Val  Leu Ser Asp 
    1175                 1180                 1185             


Phe Val  Gln Ile Tyr Asn Cys  Asn Val Thr Phe Val  Asn Ile Ser 
    1190                 1195                 1200             


Arg Val  Glu Leu His Thr Val  Ile Pro Asp Tyr Val  Asp Val Asn 
    1205                 1210                 1215             


Lys Thr  Leu Gln Glu Phe Ala  Gln Asn Leu Pro Lys  Tyr Val Lys 
    1220                 1225                 1230             


Pro Asn  Phe Asp Leu Thr Pro  Phe Asn Leu Thr Tyr  Leu Asn Leu 
    1235                 1240                 1245             


Ser Ser  Glu Leu Lys Gln Leu  Glu Ala Lys Thr Ala  Ser Leu Phe 
    1250                 1255                 1260             


Gln Thr  Thr Val Glu Leu Gln  Gly Leu Ile Asp Gln  Ile Asn Ser 
    1265                 1270                 1275             


Thr Tyr  Val Asp Leu Lys Leu  Leu Asn Arg Phe Glu  Asn Gly Gly 
    1280                 1285                 1290             


Tyr Ile  Pro Glu Ala Pro Arg  Asp Gly Gln Ala Tyr  Val Arg Lys 
    1295                 1300                 1305             


Asp Gly  Glu Trp Val Leu Leu  Ser Thr Phe 
    1310                 1315             


<210>  37
<211>  1135
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  37

Met Phe Val Leu Leu Val Ala Tyr Ala Leu Leu His Ile Ala Gly Cys 
1               5                   10                  15      


Gln Thr Thr Asn Gly Thr Asn Thr Ser His Ser Val Cys Asn Gly Cys 
            20                  25                  30          


Val Gly His Ser Glu Asn Val Phe Ala Val Glu Ser Gly Gly Tyr Ile 
        35                  40                  45              


Pro Ser Asn Phe Ala Phe Asn Asn Trp Phe Leu Leu Thr Asn Thr Ser 
    50                  55                  60                  


Ser Val Val Asp Gly Val Val Arg Ser Phe Gln Pro Leu Leu Leu Asn 
65                  70                  75                  80  


Cys Leu Trp Ser Val Ser Gly Ser Gln Phe Thr Thr Gly Phe Val Tyr 
                85                  90                  95      


Phe Asn Gly Thr Gly Arg Gly Ala Cys Lys Gly Phe Tyr Ser Asn Ala 
            100                 105                 110         


Ser Ser Asp Val Ile Arg Tyr Asn Ile Asn Phe Glu Glu Asn Leu Arg 
        115                 120                 125             


Arg Gly Thr Ile Leu Phe Lys Thr Ser Tyr Gly Ala Val Val Phe Tyr 
    130                 135                 140                 


Cys Thr Asn Asn Thr Leu Val Ser Gly Asp Ala His Ile Pro Ser Gly 
145                 150                 155                 160 


Thr Val Leu Gly Asn Phe Tyr Cys Phe Val Asn Thr Thr Ile Gly Asn 
                165                 170                 175     


Glu Thr Thr Ser Ala Phe Val Gly Ala Leu Pro Lys Thr Val Arg Glu 
            180                 185                 190         


Phe Val Ile Ser Arg Thr Gly His Phe Tyr Ile Asn Gly Tyr Arg Tyr 
        195                 200                 205             


Phe Ser Leu Gly Asp Val Glu Ala Val Asn Phe Asn Val Thr Asn Ala 
    210                 215                 220                 


Ala Thr Thr Val Cys Thr Val Ala Leu Ala Ser Tyr Ala Asp Val Leu 
225                 230                 235                 240 


Val Asn Val Ser Gln Thr Ala Ile Ala Asn Ile Ile Tyr Cys Asn Ser 
                245                 250                 255     


Val Ile Asn Arg Leu Arg Cys Asp Gln Leu Ser Phe Asp Val Pro Asp 
            260                 265                 270         


Gly Phe Tyr Ser Thr Ser Pro Ile Gln Pro Val Glu Leu Pro Val Ser 
        275                 280                 285             


Ile Val Ser Leu Pro Val Tyr His Lys His Thr Phe Ile Val Leu Tyr 
    290                 295                 300                 


Val Asn Phe Glu His Arg Arg Gly Pro Gly Lys Cys Tyr Asn Cys Arg 
305                 310                 315                 320 


Pro Ala Val Ile Asn Ile Thr Leu Ala Asn Phe Asn Glu Thr Lys Gly 
                325                 330                 335     


Pro Leu Cys Val Asp Thr Ser His Phe Thr Thr Gln Phe Val Asp Asn 
            340                 345                 350         


Val Lys Leu Ala Arg Trp Ser Ala Ser Ile Asn Thr Gly Asn Cys Pro 
        355                 360                 365             


Phe Ser Phe Gly Lys Val Asn Asn Phe Val Lys Phe Gly Ser Val Cys 
    370                 375                 380                 


Phe Ser Leu Lys Asp Ile Pro Gly Gly Cys Ala Met Pro Ile Met Ala 
385                 390                 395                 400 


Asn Leu Val Asn Ser Lys Ser His Asn Ile Gly Ser Leu Tyr Val Ser 
                405                 410                 415     


Trp Ser Asp Gly Asp Val Ile Thr Gly Val Pro Lys Pro Val Glu Gly 
            420                 425                 430         


Val Ser Ser Phe Met Asn Val Thr Leu Asn Lys Cys Thr Lys Tyr Asn 
        435                 440                 445             


Ile Tyr Asp Val Ser Gly Val Gly Val Ile Arg Ile Ser Asn Asp Thr 
    450                 455                 460                 


Phe Leu Asn Gly Ile Thr Tyr Thr Ser Thr Ser Gly Asn Leu Leu Gly 
465                 470                 475                 480 


Phe Lys Asp Val Thr Asn Gly Thr Ile Tyr Ser Ile Thr Pro Cys Asn 
                485                 490                 495     


Pro Pro Asp Gln Leu Val Val Tyr Gln Gln Ala Val Val Gly Ala Met 
            500                 505                 510         


Leu Ser Glu Asn Phe Thr Ser Tyr Gly Phe Ser Asn Val Val Glu Met 
        515                 520                 525             


Pro Lys Phe Phe Tyr Ala Ser Asn Gly Thr Tyr Asn Cys Thr Asp Ala 
    530                 535                 540                 


Val Leu Thr Tyr Ser Ser Phe Gly Val Cys Ala Asp Gly Ser Ile Ile 
545                 550                 555                 560 


Ala Val Gln Pro Arg Asn Val Ser Tyr Asp Ser Val Ser Ala Ile Val 
                565                 570                 575     


Thr Ala Asn Leu Ser Ile Pro Phe Asn Trp Thr Thr Ser Val Gln Val 
            580                 585                 590         


Glu Tyr Leu Gln Ile Thr Ser Thr Pro Ile Val Val Asp Cys Ser Thr 
        595                 600                 605             


Tyr Val Cys Asn Gly Asn Val Arg Cys Val Glu Leu Leu Lys Gln Tyr 
    610                 615                 620                 


Thr Ser Ala Cys Lys Thr Ile Glu Asp Ala Leu Arg Asn Ser Ala Met 
625                 630                 635                 640 


Leu Glu Ser Ala Asp Val Ser Glu Met Leu Thr Phe Asp Lys Lys Ala 
                645                 650                 655     


Phe Thr Leu Ala Asn Val Ser Ser Phe Gly Asp Tyr Asn Leu Ser Ser 
            660                 665                 670         


Val Ile Pro Ser Leu Pro Arg Ser Gly Ser Arg Val Ala Gly Arg Ser 
        675                 680                 685             


Ala Ile Glu Asp Ile Leu Phe Ser Lys Leu Val Thr Ser Gly Leu Gly 
    690                 695                 700                 


Thr Val Asp Ala Asp Tyr Lys Lys Cys Thr Lys Gly Leu Ser Ile Ala 
705                 710                 715                 720 


Asp Leu Ala Cys Ala Gln Tyr Tyr Asn Gly Ile Met Val Leu Pro Gly 
                725                 730                 735     


Val Ala Asp Ala Glu Arg Met Ala Met Tyr Thr Gly Ser Leu Ile Gly 
            740                 745                 750         


Gly Ile Ala Leu Gly Gly Leu Thr Ser Ala Ala Ser Ile Pro Phe Ser 
        755                 760                 765             


Leu Ala Ile Gln Ser Arg Leu Asn Tyr Val Ala Leu Gln Thr Asp Val 
    770                 775                 780                 


Leu Gln Glu Asn Gln Arg Ile Leu Ala Ala Ser Phe Asn Lys Ala Met 
785                 790                 795                 800 


Thr Asn Ile Val Asp Ala Phe Thr Gly Val Asn Asp Ala Ile Thr Gln 
                805                 810                 815     


Thr Ser Gln Ala Leu Gln Thr Val Ala Thr Ala Leu Asn Lys Ile Gln 
            820                 825                 830         


Asp Val Val Asn Gln Gln Gly Asn Ser Leu Asn His Leu Thr Ser Gln 
        835                 840                 845             


Leu Arg Gln Asn Phe Gln Ala Ile Ser Ser Ser Ile Gln Ala Ile Tyr 
    850                 855                 860                 


Asp Arg Leu Asp Pro Pro Gln Ala Asp Gln Gln Val Asp Arg Leu Ile 
865                 870                 875                 880 


Thr Gly Arg Leu Ala Ala Leu Asn Val Phe Val Ser His Thr Leu Thr 
                885                 890                 895     


Lys Tyr Thr Glu Val Arg Ala Ser Arg Gln Leu Ala Gln Gln Lys Val 
            900                 905                 910         


Asn Glu Cys Val Lys Ser Gln Ser Lys Arg Tyr Gly Phe Cys Gly Asn 
        915                 920                 925             


Gly Thr His Ile Phe Ser Leu Val Asn Ala Ala Pro Glu Gly Leu Val 
    930                 935                 940                 


Phe Leu His Thr Val Leu Leu Pro Thr Gln Tyr Lys Asp Val Glu Ala 
945                 950                 955                 960 


Trp Ser Gly Leu Cys Val Asp Gly Ile Asn Gly Tyr Val Leu Arg Gln 
                965                 970                 975     


Pro Asn Leu Ala Leu Tyr Lys Glu Gly Asn Tyr Tyr Arg Ile Thr Ser 
            980                 985                 990         


Arg Ile Met Phe Glu Pro Arg Ile  Pro Thr Ile Ala Asp  Phe Val Gln 
        995                 1000                 1005             


Ile Glu  Asn Cys Asn Val Thr  Phe Val Asn Ile Ser  Arg Ser Glu 
    1010                 1015                 1020             


Leu Gln  Thr Ile Val Pro Glu  Tyr Ile Asp Val Asn  Lys Thr Leu 
    1025                 1030                 1035             


Gln Glu  Leu Ser Tyr Lys Leu  Pro Asn Tyr Thr Val  Pro Asp Leu 
    1040                 1045                 1050             


Val Val  Glu Gln Tyr Asn Gln  Thr Ile Leu Asn Leu  Thr Ser Glu 
    1055                 1060                 1065             


Ile Ser  Thr Leu Glu Asn Lys  Ser Ala Glu Leu Asn  Tyr Thr Val 
    1070                 1075                 1080             


Gln Lys  Leu Gln Thr Leu Ile  Asp Asn Ile Asn Ser  Thr Leu Val 
    1085                 1090                 1095             


Asp Leu  Lys Trp Leu Asn Arg  Val Glu Thr Gly Gly  Tyr Ile Pro 
    1100                 1105                 1110             


Glu Ala  Pro Arg Asp Gly Gln  Ala Tyr Val Arg Lys  Asp Gly Glu 
    1115                 1120                 1125             


Trp Val  Leu Leu Ser Thr Phe  
    1130                 1135 


<210>  38
<211>  1386
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  38

Met Lys Ser Leu Thr Tyr Phe Trp Leu Phe Leu Pro Val Leu Ser Thr 
1               5                   10                  15      


Leu Ser Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe 
            20                  25                  30          


Arg Arg Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val 
        35                  40                  45              


Leu Gly Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr 
    50                  55                  60                  


Trp Tyr Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile 
65                  70                  75                  80  


Phe Val Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser 
                85                  90                  95      


Gln Glu Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala 
            100                 105                 110         


Thr Asn Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe 
        115                 120                 125             


Pro Ser Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr 
    130                 135                 140                 


Gly Arg Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Glu 
145                 150                 155                 160 


His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe 
                165                 170                 175     


Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val 
            180                 185                 190         


Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr 
        195                 200                 205             


Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly 
    210                 215                 220                 


Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn 
225                 230                 235                 240 


Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe 
                245                 250                 255     


Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Lys 
            260                 265                 270         


Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro 
        275                 280                 285             


Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp 
    290                 295                 300                 


Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Leu Arg 
305                 310                 315                 320 


Phe Asn Ile Asn Asp Ile Ser Val Ile Leu Ala Glu Gly Ser Ile Val 
                325                 330                 335     


Leu His Thr Ala Leu Gly Thr Asn Phe Ser Phe Val Cys Ser Asn Ser 
            340                 345                 350         


Ser Asn Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Thr Gln 
        355                 360                 365             


Val Pro Tyr Tyr Cys Phe Phe Lys Val Asp Thr Tyr Asn Ser Thr Val 
    370                 375                 380                 


Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile 
385                 390                 395                 400 


Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu 
                405                 410                 415     


Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp 
            420                 425                 430         


Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp 
        435                 440                 445             


Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys 
    450                 455                 460                 


Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu 
465                 470                 475                 480 


Asp Asp Gly Phe Tyr Thr Ile Ser Ser Arg Asn Leu Leu Ser His Glu 
                485                 490                 495     


Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe 
            500                 505                 510         


Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn 
        515                 520                 525             


Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val 
    530                 535                 540                 


Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser 
545                 550                 555                 560 


Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu 
                565                 570                 575     


Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr 
            580                 585                 590         


Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu 
        595                 600                 605             


Phe Gly Ser Gly Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys 
    610                 615                 620                 


Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp 
625                 630                 635                 640 


Val Ser Phe Met Thr Leu Asp Val Cys Thr Lys Tyr Thr Ile Tyr Gly 
                645                 650                 655     


Phe Lys Gly Glu Gly Ile Ile Thr Leu Thr Asn Ser Ser Phe Leu Ala 
            660                 665                 670         


Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn 
        675                 680                 685             


Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu 
    690                 695                 700                 


Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu 
705                 710                 715                 720 


Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr 
                725                 730                 735     


His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser 
            740                 745                 750         


Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln 
        755                 760                 765             


Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile 
    770                 775                 780                 


Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr 
785                 790                 795                 800 


Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn 
                805                 810                 815     


Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr 
            820                 825                 830         


Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val 
        835                 840                 845             


Asn Ser Met Leu Thr Ile Ser Asp Glu Ala Leu Gln Leu Ala Thr Ile 
    850                 855                 860                 


Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val 
865                 870                 875                 880 


Ser Val Tyr Asp Pro Ala Ser Arg Arg Val Val Gln Lys Arg Ser Phe 
                885                 890                 895     


Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr 
            900                 905                 910         


Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp 
        915                 920                 925             


Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val 
    930                 935                 940                 


Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly 
945                 950                 955                 960 


Met Val Leu Gly Gly Phe Thr Ser Ala Ala Ala Leu Pro Phe Ser Tyr 
                965                 970                 975     


Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu 
            980                 985                 990         


Gln Arg Asn Gln Gln Leu Leu Ala  Glu Ser Phe Asn Ser  Ala Ile Gly 
        995                 1000                 1005             


Asn Ile  Thr Ser Ala Phe Glu  Ser Val Lys Glu Ala  Ile Ser Gln 
    1010                 1015                 1020             


Thr Ser  Lys Gly Leu Asn Thr  Val Ala His Ala Leu  Thr Lys Val 
    1025                 1030                 1035             


Gln Glu  Val Val Asn Ser Gln  Gly Ala Ala Leu Thr  Gln Leu Thr 
    1040                 1045                 1050             


Val Gln  Leu Gln His Asn Phe  Gln Ala Ile Ser Ser  Ser Ile Asp 
    1055                 1060                 1065             


Asp Ile  Tyr Ser Arg Leu Asp  Ile Leu Ser Ala Asp  Ala Gln Val 
    1070                 1075                 1080             


Asp Arg  Leu Ile Thr Gly Arg  Leu Ser Ala Leu Asn  Ala Phe Val 
    1085                 1090                 1095             


Ala Gln  Thr Leu Thr Lys Tyr  Thr Glu Val Gln Ala  Ser Arg Lys 
    1100                 1105                 1110             


Leu Ala  Gln Gln Lys Val Asn  Glu Cys Val Lys Ser  Gln Ser Gln 
    1115                 1120                 1125             


Arg Tyr  Gly Phe Cys Gly Gly  Asp Gly Glu His Ile  Phe Ser Leu 
    1130                 1135                 1140             


Val Gln  Ala Ala Pro Gln Gly  Leu Leu Phe Leu His  Thr Val Leu 
    1145                 1150                 1155             


Val Pro  Ser Asp Phe Val Asp  Val Ile Ala Ile Ala  Gly Leu Cys 
    1160                 1165                 1170             


Val Asn  Asp Glu Ile Ala Leu  Thr Leu Arg Glu Pro  Gly Leu Val 
    1175                 1180                 1185             


Leu Phe  Thr His Glu Leu Gln  Asn His Thr Ala Thr  Glu Tyr Phe 
    1190                 1195                 1200             


Val Ser  Ser Arg Arg Met Phe  Glu Pro Arg Lys Pro  Thr Val Ser 
    1205                 1210                 1215             


Asp Phe  Val Gln Ile Glu Ser  Cys Val Val Thr Tyr  Val Asn Leu 
    1220                 1225                 1230             


Thr Arg  Asp Gln Leu Pro Asp  Val Ile Pro Asp Tyr  Ile Asp Val 
    1235                 1240                 1245             


Asn Lys  Thr Leu Tyr Glu Ile  Leu Ala Ser Leu Pro  Asn Arg Thr 
    1250                 1255                 1260             


Gly Pro  Ser Leu Pro Leu Asp  Val Phe Asn Ala Thr  Tyr Leu Asn 
    1265                 1270                 1275             


Leu Thr  Gly Glu Ile Ala Asp  Leu Glu Gln Arg Ser  Glu Ser Leu 
    1280                 1285                 1290             


Arg Asn  Thr Thr Glu Glu Leu  Gln Ser Leu Ile Tyr  Asn Ile Asn 
    1295                 1300                 1305             


Asn Thr  Leu Val Asp Leu Glu  Trp Leu Asn Arg Val  Glu Thr Tyr 
    1310                 1315                 1320             


Ile Lys  Trp Pro Trp Trp Val  Trp Leu Ile Ile Phe  Ile Val Leu 
    1325                 1330                 1335             


Ile Phe  Val Val Ser Leu Leu  Val Phe Cys Cys Ile  Ser Thr Gly 
    1340                 1345                 1350             


Cys Cys  Gly Cys Cys Gly Cys  Cys Cys Ala Cys Phe  Ser Gly Cys 
    1355                 1360                 1365             


Cys Arg  Gly Pro Arg Leu Gln  Pro Tyr Glu Val Phe  Glu Lys Val 
    1370                 1375                 1380             


His Val  Gln 
    1385     


<210>  39
<211>  1322
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  39

Met Lys Ser Leu Thr Tyr Phe Trp Leu Phe Leu Pro Val Leu Ser Thr 
1               5                   10                  15      


Leu Ser Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe 
            20                  25                  30          


Arg Arg Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val 
        35                  40                  45              


Leu Gly Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr 
    50                  55                  60                  


Trp Tyr Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile 
65                  70                  75                  80  


Phe Val Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser 
                85                  90                  95      


Gln Glu Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala 
            100                 105                 110         


Thr Asn Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe 
        115                 120                 125             


Pro Ser Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr 
    130                 135                 140                 


Gly Arg Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Glu 
145                 150                 155                 160 


His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe 
                165                 170                 175     


Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val 
            180                 185                 190         


Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr 
        195                 200                 205             


Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly 
    210                 215                 220                 


Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn 
225                 230                 235                 240 


Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe 
                245                 250                 255     


Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Lys 
            260                 265                 270         


Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro 
        275                 280                 285             


Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp 
    290                 295                 300                 


Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Leu Arg 
305                 310                 315                 320 


Phe Asn Ile Asn Asp Ile Ser Val Ile Leu Ala Glu Gly Ser Ile Val 
                325                 330                 335     


Leu His Thr Ala Leu Gly Thr Asn Phe Ser Phe Val Cys Ser Asn Ser 
            340                 345                 350         


Ser Asn Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Thr Gln 
        355                 360                 365             


Val Pro Tyr Tyr Cys Phe Phe Lys Val Asp Thr Tyr Asn Ser Thr Val 
    370                 375                 380                 


Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile 
385                 390                 395                 400 


Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu 
                405                 410                 415     


Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp 
            420                 425                 430         


Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp 
        435                 440                 445             


Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys 
    450                 455                 460                 


Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu 
465                 470                 475                 480 


Asp Asp Gly Phe Tyr Thr Ile Ser Ser Arg Asn Leu Leu Ser His Glu 
                485                 490                 495     


Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe 
            500                 505                 510         


Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn 
        515                 520                 525             


Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val 
    530                 535                 540                 


Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser 
545                 550                 555                 560 


Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu 
                565                 570                 575     


Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr 
            580                 585                 590         


Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu 
        595                 600                 605             


Phe Gly Ser Gly Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys 
    610                 615                 620                 


Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp 
625                 630                 635                 640 


Val Ser Phe Met Thr Leu Asp Val Cys Thr Lys Tyr Thr Ile Tyr Gly 
                645                 650                 655     


Phe Lys Gly Glu Gly Ile Ile Thr Leu Thr Asn Ser Ser Phe Leu Ala 
            660                 665                 670         


Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn 
        675                 680                 685             


Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu 
    690                 695                 700                 


Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu 
705                 710                 715                 720 


Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr 
                725                 730                 735     


His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser 
            740                 745                 750         


Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln 
        755                 760                 765             


Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile 
    770                 775                 780                 


Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr 
785                 790                 795                 800 


Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn 
                805                 810                 815     


Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr 
            820                 825                 830         


Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val 
        835                 840                 845             


Asn Ser Met Leu Thr Ile Ser Asp Glu Ala Leu Gln Leu Ala Thr Ile 
    850                 855                 860                 


Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val 
865                 870                 875                 880 


Ser Val Tyr Asp Pro Ala Ser Arg Arg Val Val Gln Lys Arg Ser Phe 
                885                 890                 895     


Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr 
            900                 905                 910         


Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp 
        915                 920                 925             


Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val 
    930                 935                 940                 


Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly 
945                 950                 955                 960 


Met Val Leu Gly Gly Phe Thr Ser Ala Ala Ala Leu Pro Phe Ser Tyr 
                965                 970                 975     


Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu 
            980                 985                 990         


Gln Arg Asn Gln Gln Leu Leu Ala  Glu Ser Phe Asn Ser  Ala Ile Gly 
        995                 1000                 1005             


Asn Ile  Thr Ser Ala Phe Glu  Ser Val Lys Glu Ala  Ile Ser Gln 
    1010                 1015                 1020             


Thr Ser  Lys Gly Leu Asn Thr  Val Ala His Ala Leu  Thr Lys Val 
    1025                 1030                 1035             


Gln Glu  Val Val Asn Ser Gln  Gly Ala Ala Leu Thr  Gln Leu Thr 
    1040                 1045                 1050             


Val Gln  Leu Gln His Asn Phe  Gln Ala Ile Ser Ser  Ser Ile Asp 
    1055                 1060                 1065             


Asp Ile  Tyr Ser Arg Leu Asp  Pro Pro Ser Ala Asp  Ala Gln Val 
    1070                 1075                 1080             


Asp Arg  Leu Ile Thr Gly Arg  Leu Ser Ala Leu Asn  Ala Phe Val 
    1085                 1090                 1095             


Ala Gln  Thr Leu Thr Lys Tyr  Thr Glu Val Gln Ala  Ser Arg Lys 
    1100                 1105                 1110             


Leu Ala  Gln Gln Lys Val Asn  Glu Cys Val Lys Ser  Gln Ser Gln 
    1115                 1120                 1125             


Arg Tyr  Gly Phe Cys Gly Gly  Asp Gly Glu His Ile  Phe Ser Leu 
    1130                 1135                 1140             


Val Gln  Ala Ala Pro Gln Gly  Leu Leu Phe Leu His  Thr Val Leu 
    1145                 1150                 1155             


Val Pro  Ser Asp Phe Val Asp  Val Ile Ala Ile Ala  Gly Leu Cys 
    1160                 1165                 1170             


Val Asn  Asp Glu Ile Ala Leu  Thr Leu Arg Glu Pro  Gly Leu Val 
    1175                 1180                 1185             


Leu Phe  Thr His Glu Leu Gln  Asn His Thr Ala Thr  Glu Tyr Phe 
    1190                 1195                 1200             


Val Ser  Ser Arg Arg Met Phe  Glu Pro Arg Lys Pro  Thr Val Ser 
    1205                 1210                 1215             


Asp Phe  Val Gln Ile Glu Ser  Cys Val Val Thr Tyr  Val Asn Leu 
    1220                 1225                 1230             


Thr Arg  Asp Gln Leu Pro Asp  Val Ile Pro Asp Tyr  Ile Asp Val 
    1235                 1240                 1245             


Asn Lys  Thr Leu Tyr Glu Ile  Leu Ala Ser Leu Pro  Asn Arg Thr 
    1250                 1255                 1260             


Gly Pro  Ser Leu Pro Leu Asp  Val Phe Asn Ala Thr  Tyr Leu Asn 
    1265                 1270                 1275             


Leu Thr  Gly Glu Ile Ala Asp  Leu Glu Gln Arg Ser  Glu Ser Leu 
    1280                 1285                 1290             


Arg Asn  Thr Thr Glu Glu Leu  Gln Ser Leu Ile Tyr  Asn Ile Asn 
    1295                 1300                 1305             


Asn Thr  Leu Val Asp Leu Glu  Trp Leu Asn Arg Val  Glu Thr 
    1310                 1315                 1320         


<210>  40
<211>  1349
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus spike protein

<400>  40

Met Lys Ser Leu Thr Tyr Phe Trp Leu Phe Leu Pro Val Leu Ser Thr 
1               5                   10                  15      


Leu Ser Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe 
            20                  25                  30          


Arg Arg Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val 
        35                  40                  45              


Leu Gly Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr 
    50                  55                  60                  


Trp Tyr Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile 
65                  70                  75                  80  


Phe Val Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser 
                85                  90                  95      


Gln Glu Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala 
            100                 105                 110         


Thr Asn Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe 
        115                 120                 125             


Pro Ser Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr 
    130                 135                 140                 


Gly Arg Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Glu 
145                 150                 155                 160 


His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe 
                165                 170                 175     


Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val 
            180                 185                 190         


Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr 
        195                 200                 205             


Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly 
    210                 215                 220                 


Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn 
225                 230                 235                 240 


Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe 
                245                 250                 255     


Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Lys 
            260                 265                 270         


Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro 
        275                 280                 285             


Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp 
    290                 295                 300                 


Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Leu Arg 
305                 310                 315                 320 


Phe Asn Ile Asn Asp Ile Ser Val Ile Leu Ala Glu Gly Ser Ile Val 
                325                 330                 335     


Leu His Thr Ala Leu Gly Thr Asn Phe Ser Phe Val Cys Ser Asn Ser 
            340                 345                 350         


Ser Asn Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Thr Gln 
        355                 360                 365             


Val Pro Tyr Tyr Cys Phe Phe Lys Val Asp Thr Tyr Asn Ser Thr Val 
    370                 375                 380                 


Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile 
385                 390                 395                 400 


Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu 
                405                 410                 415     


Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp 
            420                 425                 430         


Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp 
        435                 440                 445             


Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys 
    450                 455                 460                 


Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu 
465                 470                 475                 480 


Asp Asp Gly Phe Tyr Thr Ile Ser Ser Arg Asn Leu Leu Ser His Glu 
                485                 490                 495     


Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe 
            500                 505                 510         


Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn 
        515                 520                 525             


Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val 
    530                 535                 540                 


Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser 
545                 550                 555                 560 


Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu 
                565                 570                 575     


Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr 
            580                 585                 590         


Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu 
        595                 600                 605             


Phe Gly Ser Gly Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys 
    610                 615                 620                 


Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp 
625                 630                 635                 640 


Val Ser Phe Met Thr Leu Asp Val Cys Thr Lys Tyr Thr Ile Tyr Gly 
                645                 650                 655     


Phe Lys Gly Glu Gly Ile Ile Thr Leu Thr Asn Ser Ser Phe Leu Ala 
            660                 665                 670         


Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn 
        675                 680                 685             


Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu 
    690                 695                 700                 


Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu 
705                 710                 715                 720 


Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr 
                725                 730                 735     


His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser 
            740                 745                 750         


Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln 
        755                 760                 765             


Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile 
    770                 775                 780                 


Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr 
785                 790                 795                 800 


Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn 
                805                 810                 815     


Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr 
            820                 825                 830         


Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val 
        835                 840                 845             


Asn Ser Met Leu Thr Ile Ser Asp Glu Ala Leu Gln Leu Ala Thr Ile 
    850                 855                 860                 


Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val 
865                 870                 875                 880 


Ser Val Tyr Asp Pro Ala Ser Arg Arg Val Val Gln Lys Arg Ser Phe 
                885                 890                 895     


Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr 
            900                 905                 910         


Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp 
        915                 920                 925             


Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val 
    930                 935                 940                 


Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly 
945                 950                 955                 960 


Met Val Leu Gly Gly Phe Thr Ser Ala Ala Ala Leu Pro Phe Ser Tyr 
                965                 970                 975     


Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu 
            980                 985                 990         


Gln Arg Asn Gln Gln Leu Leu Ala  Glu Ser Phe Asn Ser  Ala Ile Gly 
        995                 1000                 1005             


Asn Ile  Thr Ser Ala Phe Glu  Ser Val Lys Glu Ala  Ile Ser Gln 
    1010                 1015                 1020             


Thr Ser  Lys Gly Leu Asn Thr  Val Ala His Ala Leu  Thr Lys Val 
    1025                 1030                 1035             


Gln Glu  Val Val Asn Ser Gln  Gly Ala Ala Leu Thr  Gln Leu Thr 
    1040                 1045                 1050             


Val Gln  Leu Gln His Asn Phe  Gln Ala Ile Ser Ser  Ser Ile Asp 
    1055                 1060                 1065             


Asp Ile  Tyr Ser Arg Leu Asp  Pro Pro Ser Ala Asp  Ala Gln Val 
    1070                 1075                 1080             


Asp Arg  Leu Ile Thr Gly Arg  Leu Ser Ala Leu Asn  Ala Phe Val 
    1085                 1090                 1095             


Ala Gln  Thr Leu Thr Lys Tyr  Thr Glu Val Gln Ala  Ser Arg Lys 
    1100                 1105                 1110             


Leu Ala  Gln Gln Lys Val Asn  Glu Cys Val Lys Ser  Gln Ser Gln 
    1115                 1120                 1125             


Arg Tyr  Gly Phe Cys Gly Gly  Asp Gly Glu His Ile  Phe Ser Leu 
    1130                 1135                 1140             


Val Gln  Ala Ala Pro Gln Gly  Leu Leu Phe Leu His  Thr Val Leu 
    1145                 1150                 1155             


Val Pro  Ser Asp Phe Val Asp  Val Ile Ala Ile Ala  Gly Leu Cys 
    1160                 1165                 1170             


Val Asn  Asp Glu Ile Ala Leu  Thr Leu Arg Glu Pro  Gly Leu Val 
    1175                 1180                 1185             


Leu Phe  Thr His Glu Leu Gln  Asn His Thr Ala Thr  Glu Tyr Phe 
    1190                 1195                 1200             


Val Ser  Ser Arg Arg Met Phe  Glu Pro Arg Lys Pro  Thr Val Ser 
    1205                 1210                 1215             


Asp Phe  Val Gln Ile Glu Ser  Cys Val Val Thr Tyr  Val Asn Leu 
    1220                 1225                 1230             


Thr Arg  Asp Gln Leu Pro Asp  Val Ile Pro Asp Tyr  Ile Asp Val 
    1235                 1240                 1245             


Asn Lys  Thr Leu Tyr Glu Ile  Leu Ala Ser Leu Pro  Asn Arg Thr 
    1250                 1255                 1260             


Gly Pro  Ser Leu Pro Leu Asp  Val Phe Asn Ala Thr  Tyr Leu Asn 
    1265                 1270                 1275             


Leu Thr  Gly Glu Ile Ala Asp  Leu Glu Gln Arg Ser  Glu Ser Leu 
    1280                 1285                 1290             


Arg Asn  Thr Thr Glu Glu Leu  Gln Ser Leu Ile Tyr  Asn Ile Asn 
    1295                 1300                 1305             


Asn Thr  Leu Val Asp Leu Glu  Trp Leu Asn Arg Val  Glu Thr Gly 
    1310                 1315                 1320             


Gly Tyr  Ile Pro Glu Ala Pro  Arg Asp Gly Gln Ala  Tyr Val Arg 
    1325                 1330                 1335             


Lys Asp  Gly Glu Trp Val Leu  Leu Ser Thr Phe 
    1340                 1345                 


<210>  41
<211>  1160
<212>  PRT
<213>  Swine deltacoronovirus

<400>  41

Met Gln Arg Ala Leu Leu Ile Met Thr Leu Leu Cys Leu Ala Arg Ala 
1               5                   10                  15      


Lys Phe Ala Asp Asp Leu Leu Asp Leu Leu Thr Phe Pro Gly Ala His 
            20                  25                  30          


Arg Phe Leu His Lys Pro Thr Arg Asn Asp Ser Ile Leu Tyr Ser Arg 
        35                  40                  45              


Ala Asn Asn Asn Phe Asp Val Gly Val Leu Pro Gly Tyr Pro Thr Lys 
    50                  55                  60                  


Asn Val Asn Leu Phe Ser Pro Leu Thr Asn Ser Thr Leu Pro Ile Asn 
65                  70                  75                  80  


Gly Leu His Arg Ser Tyr Gln Pro Leu Met Leu Asn Cys Leu Thr Lys 
                85                  90                  95      


Ile Thr Asn Gln Thr Leu Ser Met Tyr Leu Gln Pro Ser Glu Ile Gln 
            100                 105                 110         


Thr Tyr Ser Cys Gly Gly Ala Met Val Lys Tyr Gln Thr His Asp Ala 
        115                 120                 125             


Val Arg Ile Ile Leu Asp Leu Ile Ala Thr Asp Arg Ile Ser Val Glu 
    130                 135                 140                 


Val Val Gly Gln Ala Gly Glu Asn Tyr Val Phe Val Cys Ser Asp Gln 
145                 150                 155                 160 


Phe Asn Tyr Thr Thr Ala Leu His Asn Ser Thr Phe Phe Ser Leu Asn 
                165                 170                 175     


Ser Gln Leu Tyr Cys Phe Thr Asn Asn Thr Tyr Leu Gly Ile Leu Pro 
            180                 185                 190         


Pro Asp Leu Thr Asp Phe Thr Val Tyr Arg Thr Gly Gln Phe Tyr Ala 
        195                 200                 205             


Asn Gly Tyr Leu Leu Gly Thr Leu Pro Ile Thr Val Asn Tyr Val Arg 
    210                 215                 220                 


Leu Tyr Arg Gly Gln Leu Ser Ala Asn Ser Ala His Phe Ala Leu Ala 
225                 230                 235                 240 


Asn Leu Thr Asp Thr Leu Ile Thr Leu Thr Asn Thr Thr Ile Ser Gln 
                245                 250                 255     


Ile Thr Tyr Cys Asp Lys Ser Val Val Asp Ser Ile Ala Cys Gln Arg 
            260                 265                 270         


Ser Ser His Gln Val Glu Asp Gly Phe Tyr Ser Asp Pro Lys Ser Ala 
        275                 280                 285             


Val Arg Ala Arg Gln Arg Thr Ile Val Thr Leu Pro Lys Leu Pro Glu 
    290                 295                 300                 


Leu Glu Val Val Gln Leu Asn Ile Ser Ala His Met Asp Phe Gly Glu 
305                 310                 315                 320 


Ala Arg Leu Asp Ser Val Thr Ile Asn Gly Asn Thr Ser Tyr Cys Val 
                325                 330                 335     


Thr Lys Pro Tyr Phe Arg Leu Glu Thr Asn Phe Leu Cys Arg Gly Cys 
            340                 345                 350         


Thr Met Asn Leu Arg Thr Asp Thr Cys Ser Phe Asp Leu Ser Ala Val 
        355                 360                 365             


Asn Asn Gly Met Ser Phe Ser Gln Phe Cys Leu Ser Thr Glu Ser Gly 
    370                 375                 380                 


Ala Cys Glu Met Lys Ile Ile Val Thr Tyr Val Trp Asn Tyr Leu Leu 
385                 390                 395                 400 


Arg Gln Arg Leu Tyr Val Thr Ala Val Glu Gly Gln Thr His Thr Gly 
                405                 410                 415     


Thr Thr Ser Val His Ala Thr Asp Thr Ser Ser Val Ile Thr Asp Val 
            420                 425                 430         


Cys Thr Asp Tyr Thr Ile Tyr Gly Val Ser Gly Thr Gly Ile Ile Lys 
        435                 440                 445             


Pro Ser Asp Leu Leu Leu His Asn Gly Ile Ala Phe Thr Ser Pro Thr 
    450                 455                 460                 


Gly Glu Leu Tyr Ala Phe Lys Asn Ile Thr Thr Gly Lys Thr Leu Gln 
465                 470                 475                 480 


Val Leu Pro Cys Glu Thr Pro Ser Gln Leu Ile Val Ile Asn Asn Thr 
                485                 490                 495     


Val Val Gly Ala Ile Thr Ser Ser Asn Ser Thr Glu Asn Asn Arg Phe 
            500                 505                 510         


Thr Thr Thr Ile Val Thr Pro Thr Phe Phe Tyr Ser Thr Asn Ala Thr 
        515                 520                 525             


Thr Leu Asn Cys Thr Lys Pro Val Leu Ser Tyr Gly Pro Ile Ser Val 
    530                 535                 540                 


Cys Ser Asp Gly Ala Ile Ala Gly Thr Ser Thr Leu Gln Asn Thr Arg 
545                 550                 555                 560 


Pro Ser Ile Val Ser Leu Tyr Asp Gly Glu Ile Glu Ile Pro Ser Ala 
                565                 570                 575     


Phe Ser Leu Ser Val Gln Thr Glu Tyr Leu Gln Val Gln Ala Glu Gln 
            580                 585                 590         


Val Ile Val Asp Cys Pro Gln Tyr Val Cys Asn Gly Asn Ser Arg Cys 
        595                 600                 605             


Leu Gln Leu Leu Ala Gln Tyr Thr Ser Ala Cys Ser Asn Ile Glu Val 
    610                 615                 620                 


Ala Leu His Ser Ser Ala Gln Leu Asp Ser Arg Glu Ile Ile Ser Met 
625                 630                 635                 640 


Phe Lys Thr Ser Thr Gln Ser Leu Gln Leu Ala Asn Ile Thr Asn Phe 
                645                 650                 655     


Lys Gly Asp Tyr Asn Phe Ser Ser Ile Leu Thr Ser Arg Val Gly Gly 
            660                 665                 670         


Arg Ser Ala Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Ser Gly 
        675                 680                 685             


Leu Gly Thr Val Asp Gln Asp Tyr Lys Ser Cys Ser Arg Asn Met Ala 
    690                 695                 700                 


Ile Ala Asp Leu Val Cys Ser Gln Tyr Tyr Asn Gly Ile Met Val Leu 
705                 710                 715                 720 


Pro Gly Val Val Asp Ala Glu Lys Met Ala Met Tyr Thr Gly Ser Leu 
                725                 730                 735     


Thr Gly Ala Met Val Phe Gly Gly Leu Thr Ala Ala Ala Ala Ile Pro 
            740                 745                 750         


Phe Ala Thr Ala Val Gln Ala Arg Leu Asn Tyr Val Ala Leu Gln Thr 
        755                 760                 765             


Asn Val Leu Gln Glu Asn Gln Lys Ile Leu Ala Glu Ser Phe Asn Gln 
    770                 775                 780                 


Ala Val Gly Asn Ile Ser Leu Ala Leu Ser Ser Val Asn Asp Ala Ile 
785                 790                 795                 800 


Gln Gln Thr Ser Glu Ala Leu Asn Thr Val Ala Ile Ala Ile Lys Lys 
                805                 810                 815     


Ile Gln Thr Val Val Asn Gln Gln Gly Glu Ala Leu Ser His Leu Thr 
            820                 825                 830         


Ala Gln Leu Ser Asn Asn Phe Gln Ala Ile Ser Thr Ser Ile Gln Asp 
        835                 840                 845             


Ile Tyr Asn Arg Leu Glu Glu Val Glu Ala Asn Gln Gln Val Asp Arg 
    850                 855                 860                 


Leu Ile Asn Gly Arg Leu Ala Ala Leu Asn Ala Tyr Val Thr Gln Leu 
865                 870                 875                 880 


Leu Asn Gln Met Ser Gln Ile Arg Gln Ser Arg Leu Leu Ala Gln Gln 
                885                 890                 895     


Lys Ile Asn Glu Cys Val Lys Ser Gln Ser Pro Arg Tyr Gly Phe Cys 
            900                 905                 910         


Gly Asn Gly Thr His Ile Phe Ser Leu Thr Gln Thr Ala Pro Asn Gly 
        915                 920                 925             


Ile Phe Phe Met His Ala Val Leu Val Pro Asn Lys Phe Thr Arg Val 
    930                 935                 940                 


Asn Ala Ser Ala Gly Ile Cys Val Asp Asn Thr Arg Gly Tyr Ser Leu 
945                 950                 955                 960 


Gln Pro Gln Leu Ile Leu Tyr Gln Phe Asn Asn Ser Trp Arg Val Thr 
                965                 970                 975     


Pro Arg Asn Met Tyr Glu Pro Arg Leu Pro Arg Gln Ala Asp Phe Ile 
            980                 985                 990         


Gln Leu Thr Asp Cys Ser Val Thr  Phe Tyr Asn Thr Thr  Ala Ala Asn 
        995                 1000                 1005             


Leu Pro  Asn Ile Ile Pro Asp  Val Ile Asp Val Asn  Gln Thr Val 
    1010                 1015                 1020             


Ser Asp  Ile Ile Asp Asn Leu  Pro Thr Ala Thr Pro  Pro Gln Trp 
    1025                 1030                 1035             


Asp Val  Gly Ile Tyr Asn Asn  Thr Ile Leu Asn Leu  Thr Val Glu 
    1040                 1045                 1050             


Ile Asn  Asp Leu Gln Glu Arg  Ser Lys Asn Leu Ser  Gln Ile Ala 
    1055                 1060                 1065             


Asp Arg  Leu Gln Asn Tyr Ile  Asp Asn Leu Asn Asn  Thr Leu Val 
    1070                 1075                 1080             


Asp Leu  Glu Trp Leu Asn Arg  Val Glu Thr Tyr Leu  Lys Trp Pro 
    1085                 1090                 1095             


Trp Tyr  Ile Trp Leu Ala Ile  Ala Leu Ala Leu Ile  Ala Phe Val 
    1100                 1105                 1110             


Thr Ile  Leu Ile Thr Ile Phe  Leu Cys Thr Gly Cys  Cys Gly Gly 
    1115                 1120                 1125             


Cys Phe  Gly Cys Cys Gly Gly  Cys Phe Gly Leu Phe  Ser Lys Lys 
    1130                 1135                 1140             


Lys Arg  Tyr Thr Asp Asp Gln  Pro Thr Pro Ser Phe  Lys Phe Lys 
    1145                 1150                 1155             


Glu Trp  
    1160 


<210>  42
<211>  1093
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus S protein

<400>  42

Met Gln Arg Ala Leu Leu Ile Met Thr Leu Leu Cys Leu Ala Arg Ala 
1               5                   10                  15      


Lys Phe Ala Asp Asp Leu Leu Asp Leu Leu Thr Phe Pro Gly Ala His 
            20                  25                  30          


Arg Phe Leu His Lys Pro Thr Arg Asn Asp Ser Ile Leu Tyr Ser Arg 
        35                  40                  45              


Ala Asn Asn Asn Phe Asp Val Gly Val Leu Pro Gly Tyr Pro Thr Lys 
    50                  55                  60                  


Asn Val Asn Leu Phe Ser Pro Leu Thr Asn Ser Thr Leu Pro Ile Asn 
65                  70                  75                  80  


Gly Leu His Arg Ser Tyr Gln Pro Leu Met Leu Asn Cys Leu Thr Lys 
                85                  90                  95      


Ile Thr Asn Gln Thr Leu Ser Met Tyr Leu Gln Pro Ser Glu Ile Gln 
            100                 105                 110         


Thr Tyr Ser Cys Gly Gly Ala Met Val Lys Tyr Gln Thr His Asp Ala 
        115                 120                 125             


Val Arg Ile Ile Leu Asp Leu Ile Ala Thr Asp Arg Ile Ser Val Glu 
    130                 135                 140                 


Val Val Gly Gln Ala Gly Glu Asn Tyr Val Phe Val Cys Ser Asp Gln 
145                 150                 155                 160 


Phe Asn Tyr Thr Thr Ala Leu His Asn Ser Thr Phe Phe Ser Leu Asn 
                165                 170                 175     


Ser Gln Leu Tyr Cys Phe Thr Asn Asn Thr Tyr Leu Gly Ile Leu Pro 
            180                 185                 190         


Pro Asp Leu Thr Asp Phe Thr Val Tyr Arg Thr Gly Gln Phe Tyr Ala 
        195                 200                 205             


Asn Gly Tyr Leu Leu Gly Thr Leu Pro Ile Thr Val Asn Tyr Val Arg 
    210                 215                 220                 


Leu Tyr Arg Gly Gln Leu Ser Ala Asn Ser Ala His Phe Ala Leu Ala 
225                 230                 235                 240 


Asn Leu Thr Asp Thr Leu Ile Thr Leu Thr Asn Thr Thr Ile Ser Gln 
                245                 250                 255     


Ile Thr Tyr Cys Asp Lys Ser Val Val Asp Ser Ile Ala Cys Gln Arg 
            260                 265                 270         


Ser Ser His Gln Val Glu Asp Gly Phe Tyr Ser Asp Pro Lys Ser Ala 
        275                 280                 285             


Val Arg Ala Arg Gln Arg Thr Ile Val Thr Leu Pro Lys Leu Pro Glu 
    290                 295                 300                 


Leu Glu Val Val Gln Leu Asn Ile Ser Ala His Met Asp Phe Gly Glu 
305                 310                 315                 320 


Ala Arg Leu Asp Ser Val Thr Ile Asn Gly Asn Thr Ser Tyr Cys Val 
                325                 330                 335     


Thr Lys Pro Tyr Phe Arg Leu Glu Thr Asn Phe Leu Cys Arg Gly Cys 
            340                 345                 350         


Thr Met Asn Leu Arg Thr Asp Thr Cys Ser Phe Asp Leu Ser Ala Val 
        355                 360                 365             


Asn Asn Gly Met Ser Phe Ser Gln Phe Cys Leu Ser Thr Glu Ser Gly 
    370                 375                 380                 


Ala Cys Glu Met Lys Ile Ile Val Thr Tyr Val Trp Asn Tyr Leu Leu 
385                 390                 395                 400 


Arg Gln Arg Leu Tyr Val Thr Ala Val Glu Gly Gln Thr His Thr Gly 
                405                 410                 415     


Thr Thr Ser Val His Ala Thr Asp Thr Ser Ser Val Ile Thr Asp Val 
            420                 425                 430         


Cys Thr Asp Tyr Thr Ile Tyr Gly Val Ser Gly Thr Gly Ile Ile Lys 
        435                 440                 445             


Pro Ser Asp Leu Leu Leu His Asn Gly Ile Ala Phe Thr Ser Pro Thr 
    450                 455                 460                 


Gly Glu Leu Tyr Ala Phe Lys Asn Ile Thr Thr Gly Lys Thr Leu Gln 
465                 470                 475                 480 


Val Leu Pro Cys Glu Thr Pro Ser Gln Leu Ile Val Ile Asn Asn Thr 
                485                 490                 495     


Val Val Gly Ala Ile Thr Ser Ser Asn Ser Thr Glu Asn Asn Arg Phe 
            500                 505                 510         


Thr Thr Thr Ile Val Thr Pro Thr Phe Phe Tyr Ser Thr Asn Ala Thr 
        515                 520                 525             


Thr Leu Asn Cys Thr Lys Pro Val Leu Ser Tyr Gly Pro Ile Ser Val 
    530                 535                 540                 


Cys Ser Asp Gly Ala Ile Ala Gly Thr Ser Thr Leu Gln Asn Thr Arg 
545                 550                 555                 560 


Pro Ser Ile Val Ser Leu Tyr Asp Gly Glu Ile Glu Ile Pro Ser Ala 
                565                 570                 575     


Phe Ser Leu Ser Val Gln Thr Glu Tyr Leu Gln Val Gln Ala Glu Gln 
            580                 585                 590         


Val Ile Val Asp Cys Pro Gln Tyr Val Cys Asn Gly Asn Ser Arg Cys 
        595                 600                 605             


Leu Gln Leu Leu Ala Gln Tyr Thr Ser Ala Cys Ser Asn Ile Glu Val 
    610                 615                 620                 


Ala Leu His Ser Ser Ala Gln Leu Asp Ser Arg Glu Ile Ile Ser Met 
625                 630                 635                 640 


Phe Lys Thr Ser Thr Gln Ser Leu Gln Leu Ala Asn Ile Thr Asn Phe 
                645                 650                 655     


Lys Gly Asp Tyr Asn Phe Ser Ser Ile Leu Thr Ser Arg Val Gly Gly 
            660                 665                 670         


Arg Ser Ala Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Ser Gly 
        675                 680                 685             


Leu Gly Thr Val Asp Gln Asp Tyr Lys Ser Cys Ser Arg Asn Met Ala 
    690                 695                 700                 


Ile Ala Asp Leu Val Cys Ser Gln Tyr Tyr Asn Gly Ile Met Val Leu 
705                 710                 715                 720 


Pro Gly Val Val Asp Ala Glu Lys Met Ala Met Tyr Thr Gly Ser Leu 
                725                 730                 735     


Thr Gly Ala Met Val Phe Gly Gly Leu Thr Ala Ala Ala Ala Ile Pro 
            740                 745                 750         


Phe Ala Thr Ala Val Gln Ala Arg Leu Asn Tyr Val Ala Leu Gln Thr 
        755                 760                 765             


Asn Val Leu Gln Glu Asn Gln Lys Ile Leu Ala Glu Ser Phe Asn Gln 
    770                 775                 780                 


Ala Val Gly Asn Ile Ser Leu Ala Leu Ser Ser Val Asn Asp Ala Ile 
785                 790                 795                 800 


Gln Gln Thr Ser Glu Ala Leu Asn Thr Val Ala Ile Ala Ile Lys Lys 
                805                 810                 815     


Ile Gln Thr Val Val Asn Gln Gln Gly Glu Ala Leu Ser His Leu Thr 
            820                 825                 830         


Ala Gln Leu Ser Asn Asn Phe Gln Ala Ile Ser Thr Ser Ile Gln Asp 
        835                 840                 845             


Ile Tyr Asn Arg Leu Glu Pro Pro Glu Ala Asn Gln Gln Val Asp Arg 
    850                 855                 860                 


Leu Ile Asn Gly Arg Leu Ala Ala Leu Asn Ala Tyr Val Thr Gln Leu 
865                 870                 875                 880 


Leu Asn Gln Met Ser Gln Ile Arg Gln Ser Arg Leu Leu Ala Gln Gln 
                885                 890                 895     


Lys Ile Asn Glu Cys Val Lys Ser Gln Ser Pro Arg Tyr Gly Phe Cys 
            900                 905                 910         


Gly Asn Gly Thr His Ile Phe Ser Leu Thr Gln Thr Ala Pro Asn Gly 
        915                 920                 925             


Ile Phe Phe Met His Ala Val Leu Val Pro Asn Lys Phe Thr Arg Val 
    930                 935                 940                 


Asn Ala Ser Ala Gly Ile Cys Val Asp Asn Thr Arg Gly Tyr Ser Leu 
945                 950                 955                 960 


Gln Pro Gln Leu Ile Leu Tyr Gln Phe Asn Asn Ser Trp Arg Val Thr 
                965                 970                 975     


Pro Arg Asn Met Tyr Glu Pro Arg Leu Pro Arg Gln Ala Asp Phe Ile 
            980                 985                 990         


Gln Leu Thr Asp Cys Ser Val Thr  Phe Tyr Asn Thr Thr  Ala Ala Asn 
        995                 1000                 1005             


Leu Pro  Asn Ile Ile Pro Asp  Val Ile Asp Val Asn  Gln Thr Val 
    1010                 1015                 1020             


Ser Asp  Ile Ile Asp Asn Leu  Pro Thr Ala Thr Pro  Pro Gln Trp 
    1025                 1030                 1035             


Asp Val  Gly Ile Tyr Asn Asn  Thr Ile Leu Asn Leu  Thr Val Glu 
    1040                 1045                 1050             


Ile Asn  Asp Leu Gln Glu Arg  Ser Lys Asn Leu Ser  Gln Ile Ala 
    1055                 1060                 1065             


Asp Arg  Leu Gln Asn Tyr Ile  Asp Asn Leu Asn Asn  Thr Leu Val 
    1070                 1075                 1080             


Asp Leu  Glu Trp Leu Asn Arg  Val Glu Thr 
    1085                 1090             


<210>  43
<211>  1120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant coronavirus S protein

<400>  43

Met Gln Arg Ala Leu Leu Ile Met Thr Leu Leu Cys Leu Ala Arg Ala 
1               5                   10                  15      


Lys Phe Ala Asp Asp Leu Leu Asp Leu Leu Thr Phe Pro Gly Ala His 
            20                  25                  30          


Arg Phe Leu His Lys Pro Thr Arg Asn Asp Ser Ile Leu Tyr Ser Arg 
        35                  40                  45              


Ala Asn Asn Asn Phe Asp Val Gly Val Leu Pro Gly Tyr Pro Thr Lys 
    50                  55                  60                  


Asn Val Asn Leu Phe Ser Pro Leu Thr Asn Ser Thr Leu Pro Ile Asn 
65                  70                  75                  80  


Gly Leu His Arg Ser Tyr Gln Pro Leu Met Leu Asn Cys Leu Thr Lys 
                85                  90                  95      


Ile Thr Asn Gln Thr Leu Ser Met Tyr Leu Gln Pro Ser Glu Ile Gln 
            100                 105                 110         


Thr Tyr Ser Cys Gly Gly Ala Met Val Lys Tyr Gln Thr His Asp Ala 
        115                 120                 125             


Val Arg Ile Ile Leu Asp Leu Ile Ala Thr Asp Arg Ile Ser Val Glu 
    130                 135                 140                 


Val Val Gly Gln Ala Gly Glu Asn Tyr Val Phe Val Cys Ser Asp Gln 
145                 150                 155                 160 


Phe Asn Tyr Thr Thr Ala Leu His Asn Ser Thr Phe Phe Ser Leu Asn 
                165                 170                 175     


Ser Gln Leu Tyr Cys Phe Thr Asn Asn Thr Tyr Leu Gly Ile Leu Pro 
            180                 185                 190         


Pro Asp Leu Thr Asp Phe Thr Val Tyr Arg Thr Gly Gln Phe Tyr Ala 
        195                 200                 205             


Asn Gly Tyr Leu Leu Gly Thr Leu Pro Ile Thr Val Asn Tyr Val Arg 
    210                 215                 220                 


Leu Tyr Arg Gly Gln Leu Ser Ala Asn Ser Ala His Phe Ala Leu Ala 
225                 230                 235                 240 


Asn Leu Thr Asp Thr Leu Ile Thr Leu Thr Asn Thr Thr Ile Ser Gln 
                245                 250                 255     


Ile Thr Tyr Cys Asp Lys Ser Val Val Asp Ser Ile Ala Cys Gln Arg 
            260                 265                 270         


Ser Ser His Gln Val Glu Asp Gly Phe Tyr Ser Asp Pro Lys Ser Ala 
        275                 280                 285             


Val Arg Ala Arg Gln Arg Thr Ile Val Thr Leu Pro Lys Leu Pro Glu 
    290                 295                 300                 


Leu Glu Val Val Gln Leu Asn Ile Ser Ala His Met Asp Phe Gly Glu 
305                 310                 315                 320 


Ala Arg Leu Asp Ser Val Thr Ile Asn Gly Asn Thr Ser Tyr Cys Val 
                325                 330                 335     


Thr Lys Pro Tyr Phe Arg Leu Glu Thr Asn Phe Leu Cys Arg Gly Cys 
            340                 345                 350         


Thr Met Asn Leu Arg Thr Asp Thr Cys Ser Phe Asp Leu Ser Ala Val 
        355                 360                 365             


Asn Asn Gly Met Ser Phe Ser Gln Phe Cys Leu Ser Thr Glu Ser Gly 
    370                 375                 380                 


Ala Cys Glu Met Lys Ile Ile Val Thr Tyr Val Trp Asn Tyr Leu Leu 
385                 390                 395                 400 


Arg Gln Arg Leu Tyr Val Thr Ala Val Glu Gly Gln Thr His Thr Gly 
                405                 410                 415     


Thr Thr Ser Val His Ala Thr Asp Thr Ser Ser Val Ile Thr Asp Val 
            420                 425                 430         


Cys Thr Asp Tyr Thr Ile Tyr Gly Val Ser Gly Thr Gly Ile Ile Lys 
        435                 440                 445             


Pro Ser Asp Leu Leu Leu His Asn Gly Ile Ala Phe Thr Ser Pro Thr 
    450                 455                 460                 


Gly Glu Leu Tyr Ala Phe Lys Asn Ile Thr Thr Gly Lys Thr Leu Gln 
465                 470                 475                 480 


Val Leu Pro Cys Glu Thr Pro Ser Gln Leu Ile Val Ile Asn Asn Thr 
                485                 490                 495     


Val Val Gly Ala Ile Thr Ser Ser Asn Ser Thr Glu Asn Asn Arg Phe 
            500                 505                 510         


Thr Thr Thr Ile Val Thr Pro Thr Phe Phe Tyr Ser Thr Asn Ala Thr 
        515                 520                 525             


Thr Leu Asn Cys Thr Lys Pro Val Leu Ser Tyr Gly Pro Ile Ser Val 
    530                 535                 540                 


Cys Ser Asp Gly Ala Ile Ala Gly Thr Ser Thr Leu Gln Asn Thr Arg 
545                 550                 555                 560 


Pro Ser Ile Val Ser Leu Tyr Asp Gly Glu Ile Glu Ile Pro Ser Ala 
                565                 570                 575     


Phe Ser Leu Ser Val Gln Thr Glu Tyr Leu Gln Val Gln Ala Glu Gln 
            580                 585                 590         


Val Ile Val Asp Cys Pro Gln Tyr Val Cys Asn Gly Asn Ser Arg Cys 
        595                 600                 605             


Leu Gln Leu Leu Ala Gln Tyr Thr Ser Ala Cys Ser Asn Ile Glu Val 
    610                 615                 620                 


Ala Leu His Ser Ser Ala Gln Leu Asp Ser Arg Glu Ile Ile Ser Met 
625                 630                 635                 640 


Phe Lys Thr Ser Thr Gln Ser Leu Gln Leu Ala Asn Ile Thr Asn Phe 
                645                 650                 655     


Lys Gly Asp Tyr Asn Phe Ser Ser Ile Leu Thr Ser Arg Val Gly Gly 
            660                 665                 670         


Arg Ser Ala Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Ser Gly 
        675                 680                 685             


Leu Gly Thr Val Asp Gln Asp Tyr Lys Ser Cys Ser Arg Asn Met Ala 
    690                 695                 700                 


Ile Ala Asp Leu Val Cys Ser Gln Tyr Tyr Asn Gly Ile Met Val Leu 
705                 710                 715                 720 


Pro Gly Val Val Asp Ala Glu Lys Met Ala Met Tyr Thr Gly Ser Leu 
                725                 730                 735     


Thr Gly Ala Met Val Phe Gly Gly Leu Thr Ala Ala Ala Ala Ile Pro 
            740                 745                 750         


Phe Ala Thr Ala Val Gln Ala Arg Leu Asn Tyr Val Ala Leu Gln Thr 
        755                 760                 765             


Asn Val Leu Gln Glu Asn Gln Lys Ile Leu Ala Glu Ser Phe Asn Gln 
    770                 775                 780                 


Ala Val Gly Asn Ile Ser Leu Ala Leu Ser Ser Val Asn Asp Ala Ile 
785                 790                 795                 800 


Gln Gln Thr Ser Glu Ala Leu Asn Thr Val Ala Ile Ala Ile Lys Lys 
                805                 810                 815     


Ile Gln Thr Val Val Asn Gln Gln Gly Glu Ala Leu Ser His Leu Thr 
            820                 825                 830         


Ala Gln Leu Ser Asn Asn Phe Gln Ala Ile Ser Thr Ser Ile Gln Asp 
        835                 840                 845             


Ile Tyr Asn Arg Leu Glu Pro Pro Glu Ala Asn Gln Gln Val Asp Arg 
    850                 855                 860                 


Leu Ile Asn Gly Arg Leu Ala Ala Leu Asn Ala Tyr Val Thr Gln Leu 
865                 870                 875                 880 


Leu Asn Gln Met Ser Gln Ile Arg Gln Ser Arg Leu Leu Ala Gln Gln 
                885                 890                 895     


Lys Ile Asn Glu Cys Val Lys Ser Gln Ser Pro Arg Tyr Gly Phe Cys 
            900                 905                 910         


Gly Asn Gly Thr His Ile Phe Ser Leu Thr Gln Thr Ala Pro Asn Gly 
        915                 920                 925             


Ile Phe Phe Met His Ala Val Leu Val Pro Asn Lys Phe Thr Arg Val 
    930                 935                 940                 


Asn Ala Ser Ala Gly Ile Cys Val Asp Asn Thr Arg Gly Tyr Ser Leu 
945                 950                 955                 960 


Gln Pro Gln Leu Ile Leu Tyr Gln Phe Asn Asn Ser Trp Arg Val Thr 
                965                 970                 975     


Pro Arg Asn Met Tyr Glu Pro Arg Leu Pro Arg Gln Ala Asp Phe Ile 
            980                 985                 990         


Gln Leu Thr Asp Cys Ser Val Thr  Phe Tyr Asn Thr Thr  Ala Ala Asn 
        995                 1000                 1005             


Leu Pro  Asn Ile Ile Pro Asp  Val Ile Asp Val Asn  Gln Thr Val 
    1010                 1015                 1020             


Ser Asp  Ile Ile Asp Asn Leu  Pro Thr Ala Thr Pro  Pro Gln Trp 
    1025                 1030                 1035             


Asp Val  Gly Ile Tyr Asn Asn  Thr Ile Leu Asn Leu  Thr Val Glu 
    1040                 1045                 1050             


Ile Asn  Asp Leu Gln Glu Arg  Ser Lys Asn Leu Ser  Gln Ile Ala 
    1055                 1060                 1065             


Asp Arg  Leu Gln Asn Tyr Ile  Asp Asn Leu Asn Asn  Thr Leu Val 
    1070                 1075                 1080             


Asp Leu  Glu Trp Leu Asn Arg  Val Glu Thr Gly Gly  Tyr Ile Pro 
    1085                 1090                 1095             


Glu Ala  Pro Arg Asp Gly Gln  Ala Tyr Val Arg Lys  Asp Gly Glu 
    1100                 1105                 1110             


Trp Val  Leu Leu Ser Thr Phe  
    1115                 1120 


