                         SEQUENCE LISTING

<110>  TriBiotica LLC
       Dunn, Ian
       Lawler, Matthew
 
<120>  Methods For Directed Folding Assembly Or Dimerization Of Proteins
        By Templated Assembly Reactions

<130>  189156.00602

<150>  62424642
<151>  2016-11-21

<160>  54    

<170>  PatentIn version 3.5

<210>  1
<211>  47
<212>  PRT
<213>  artificial sequence

<220>
<223>  N-terminal conjugates

<400>  1

Cys Ser Gly Gly Ala Ser Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys 
1               5                   10                  15      

Val Lys Thr Leu Lys Ala Gln Asn Ser Glu Leu Ala Ser Thr Ala Asn 
            20                  25                  30          

Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln Lys Gly Ala Pro 
        35                  40                  45          


<210>  2
<211>  49
<212>  PRT
<213>  artificial sequence

<220>
<223>  C-terminal conjugate

<400>  2

Ser Gly Ala Ser Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys Val Lys 
1               5                   10                  15      

Thr Leu Lys Ala Gln Asn Ser Glu Leu Ala Ser Thr Ala Asn Met Leu 
            20                  25                  30          

Arg Glu Gln Val Ala Gln Leu Lys Gln Lys Gly Ala Pro Ser Gly Gly 
        35                  40                  45              

Cys 
 

<210>  3
<211>  41
<212>  PRT
<213>  artificial sequence

<220>
<223>  fos zipper

<400>  3

Ala Ser Arg Glu Leu Thr Asp Thr Leu Gln Ala Glu Thr Asp Gln Leu 
1               5                   10                  15      

Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala Asn Leu Leu Lys 
            20                  25                  30          

Glu Lys Glu Lys Leu Glu Gly Ala Pro 
        35                  40      


<210>  4
<211>  47
<212>  PRT
<213>  artificial sequence

<220>
<223>  mutant of c-Jun

<400>  4

Cys Ser Gly Gly Ala Ser Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys 
1               5                   10                  15      

Val Lys Ser Phe Lys Ala Gln Asn Ser Glu Asn Ala Ser Thr Ala Asn 
            20                  25                  30          

Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln Lys Gly Ala Pro 
        35                  40                  45          


<210>  5
<211>  49
<212>  PRT
<213>  artificial sequence

<220>
<223>  modified sequence with C-terminal cysteine residue

<400>  5

Ser Gly Ala Ser Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys Val Lys 
1               5                   10                  15      

Ser Phe Lys Ala Gln Asn Ser Glu Asn Ala Ser Thr Ala Asn Met Leu 
            20                  25                  30          

Arg Glu Gln Val Ala Gln Leu Lys Gln Lys Gly Ala Pro Ser Gly Gly 
        35                  40                  45              

Cys 
    

<210>  6
<211>  60
<212>  DNA
<213>  artificial sequence

<220>
<223>  bottle haplomer

<400>  6
actcgagacg tctccttgtc tttgcttttc ttcaggacac agtggcgaga cgtctcgagt  60


<210>  7
<211>  51
<212>  DNA
<213>  artificial sequence

<220>
<223>  bottle haplomer

<400>  7
actcgagacg tctccttcct gcccctcctc ctgctccgag acgtctcgag t           51


<210>  8
<211>  11
<212>  DNA
<213>  artificial sequence

<220>
<223>  second haplomer-ligand

<400>  8
agctctcgag t                                                       11


<210>  9
<211>  12
<212>  DNA
<213>  artificial sequence

<220>
<223>  second haplomer-ligand

<400>  9
gacgtctcga gt                                                      12


<210>  10
<211>  29
<212>  PRT
<213>  artificial sequence

<220>
<223>  NZ domain

<400>  10

Ala Leu Lys Lys Glu Leu Gln Ala Asn Lys Lys Glu Leu Ala Gln Leu 
1               5                   10                  15      

Lys Trp Glu Leu Gln Ala Leu Lys Lys Glu Leu Ala Gln 
            20                  25                  


<210>  11
<211>  30
<212>  PRT
<213>  artificial sequence

<220>
<223>  CZ domain

<400>  11

Glu Gln Leu Glu Lys Lys Leu Gln Ala Leu Glu Lys Lys Leu Ala Gln 
1               5                   10                  15      

Leu Glu Trp Lys Asn Gln Ala Leu Glu Lys Lys Leu Ala Gln 
            20                  25                  30  


<210>  12
<211>  46
<212>  PRT
<213>  artificial sequence

<220>
<223>  c-jun domain

<400>  12

Cys Ser Gly Ala Ser Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys Val 
1               5                   10                  15      

Lys Ser Phe Lys Ala Gln Asn Ser Glu Asn Ala Ser Thr Ala Asn Met 
            20                  25                  30          

Leu Arg Glu Gln Val Ala Gln Leu Lys Gln Lys Gly Ala Pro 
        35                  40                  45      


<210>  13
<211>  43
<212>  PRT
<213>  artificial sequence

<220>
<223>  c-Fos domain

<400>  13

Ser Gly Ala Ser Arg Glu Leu Thr Asp Thr Leu Gln Ala Glu Thr Asp 
1               5                   10                  15      

Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala Asn Leu 
            20                  25                  30          

Leu Lys Glu Lys Glu Lys Leu Glu Gly Ala Pro 
        35                  40              


<210>  14
<211>  107
<212>  PRT
<213>  artificial sequence

<220>
<223>  F36V FKBP mutant domain

<400>  14

Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
1               5                   10                  15      

Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
            20                  25                  30          

Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
        35                  40                  45              

Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
    50                  55                  60                  

Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
65                  70                  75                  80  

Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
                85                  90                  95      

Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
            100                 105         


<210>  15
<211>  108
<212>  PRT
<213>  artificial sequence

<220>
<223>  F36V FKBP mutant domain

<400>  15

Met Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe 
1               5                   10                  15      

Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu 
            20                  25                  30          

Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys 
        35                  40                  45              

Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val 
    50                  55                  60                  

Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp 
65                  70                  75                  80  

Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala 
                85                  90                  95      

Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
            100                 105             


<210>  16
<211>  43
<212>  PRT
<213>  artificial sequence

<220>
<223>  c-jun domain

<400>  16

Ala Ser Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Ser Phe 
1               5                   10                  15      

Lys Ala Gln Asn Ser Glu Asn Ala Ser Thr Ala Asn Met Leu Arg Glu 
            20                  25                  30          

Gln Val Ala Gln Leu Lys Gln Lys Gly Ala Pro 
        35                  40              


<210>  17
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  linker

<400>  17

Ala Gly Ser Ser Ala Ala Gly Ser Gly Ser 
1               5                   10  


<210>  18
<211>  18
<212>  PRT
<213>  artificial sequence

<220>
<223>  linker

<400>  18

Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly 
1               5                   10                  15      

Gly Gly 
        

<210>  19
<211>  22
<212>  PRT
<213>  artificial sequence

<220>
<223>  linker

<400>  19

Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly 
1               5                   10                  15      

Gly Gly Ser Gly Gly Gly 
            20          


<210>  20
<211>  26
<212>  PRT
<213>  artificial sequence

<220>
<223>  linker

<400>  20

Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly 
1               5                   10                  15      

Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
            20                  25      


<210>  21
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  linker

<400>  21

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10                  15  


<210>  22
<211>  20
<212>  PRT
<213>  artificial sequence

<220>
<223>  Linker

<400>  22

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15      

Gly Gly Gly Gly 
            20  


<210>  23
<211>  25
<212>  PRT
<213>  artificial sequence

<220>
<223>  Linker

<400>  23

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15      

Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            20                  25  


<210>  24
<211>  5
<212>  PRT
<213>  artificial sequence

<220>
<223>  Linker

<400>  24

Ser Gly Gly Gly Ser 
1               5   


<210>  25
<211>  4
<212>  PRT
<213>  artificial sequence

<220>
<223>  Linker

<400>  25

Ser Gly Ser Gly 
1               


<210>  26
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  Linker

<400>  26

Ser Gly Gly Gly Gly Ser 
1               5       


<210>  27
<211>  5
<212>  PRT
<213>  artificial sequence

<220>
<223>  Linker

<400>  27

Ser Gly Ser Gly Gly 
1               5   


<210>  28
<211>  14
<212>  PRT
<213>  artificial sequence

<220>
<223>  pro-apoptotic peptide

<400>  28

Lys Leu Ala Lys Leu Ala Lys Lys Leu Ala Lys Leu Ala Lys 
1               5                   10                  


<210>  29
<211>  157
<212>  PRT
<213>  artificial sequence

<220>
<223>  fragment of superfolder GFP (sfGFP)

<400>  29

Met Arg Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      

Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly Glu 
            20                  25                  30          

Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              

Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  

Thr Tyr Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  

His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      

Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         

Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             

Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 

Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln 
145                 150                 155         


<210>  30
<211>  81
<212>  PRT
<213>  artificial sequence

<220>
<223>  fragment of superfolder GFP (sfGFP)

<400>  30

Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp 
1               5                   10                  15      

Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly 
            20                  25                  30          

Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser 
        35                  40                  45              

Val Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu 
    50                  55                  60                  

Glu Phe Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr 
65                  70                  75                  80  

Lys 
    

<210>  31
<211>  229
<212>  PRT
<213>  artificial sequence

<220>
<223>  Renilla luciferase

<400>  31

Met Ala Ser Lys Val Tyr Asp Pro Glu Gln Arg Lys Arg Met Ile Thr 
1               5                   10                  15      

Gly Pro Gln Trp Trp Ala Arg Cys Lys Gln Met Asn Val Leu Asp Ser 
            20                  25                  30          

Phe Ile Asn Tyr Tyr Asp Ser Glu Lys His Ala Glu Asn Ala Val Ile 
        35                  40                  45              

Phe Leu His Gly Asn Ala Ala Ser Ser Tyr Leu Trp Arg His Val Val 
    50                  55                  60                  

Pro His Ile Glu Pro Val Ala Arg Cys Ile Ile Pro Asp Leu Ile Gly 
65                  70                  75                  80  

Met Gly Lys Ser Gly Lys Ser Gly Asn Gly Ser Tyr Arg Leu Leu Asp 
                85                  90                  95      

His Tyr Lys Tyr Leu Thr Ala Trp Phe Glu Leu Leu Asn Leu Pro Lys 
            100                 105                 110         

Lys Ile Ile Phe Val Gly His Asp Trp Gly Ala Cys Leu Ala Phe His 
        115                 120                 125             

Tyr Ser Tyr Glu His Gln Asp Lys Ile Lys Ala Ile Val His Ala Glu 
    130                 135                 140                 

Ser Val Val Asp Val Ile Glu Ser Trp Asp Glu Trp Pro Asp Ile Glu 
145                 150                 155                 160 

Glu Asp Ile Ala Leu Ile Lys Ser Glu Glu Gly Glu Lys Met Val Leu 
                165                 170                 175     

Glu Asn Asn Phe Phe Val Glu Thr Met Leu Pro Ser Lys Ile Met Arg 
            180                 185                 190         

Lys Leu Glu Pro Glu Glu Phe Ala Ala Tyr Leu Glu Pro Phe Lys Glu 
        195                 200                 205             

Lys Gly Glu Val Arg Arg Pro Thr Leu Ser Trp Pro Arg Glu Ile Pro 
    210                 215                 220                 

Leu Val Lys Gly Gly 
225                 


<210>  32
<211>  82
<212>  PRT
<213>  artificial sequence

<220>
<223>  Renilla luciferase

<400>  32

Lys Pro Asp Val Val Gln Ile Val Arg Asn Tyr Asn Ala Tyr Leu Arg 
1               5                   10                  15      

Ala Ser Asp Asp Leu Pro Lys Met Phe Ile Glu Ser Asp Pro Gly Phe 
            20                  25                  30          

Phe Ser Asn Ala Ile Val Glu Gly Ala Lys Lys Phe Pro Asn Thr Glu 
        35                  40                  45              

Phe Val Lys Val Lys Gly Leu His Phe Ser Gln Glu Asp Ala Pro Asp 
    50                  55                  60                  

Glu Met Gly Lys Tyr Ile Lys Ser Phe Val Glu Arg Val Leu Lys Asn 
65                  70                  75                  80  

Glu Gln 
        

<210>  33
<211>  34
<212>  PRT
<213>  S. cerevisiae

<400>  33

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      

Val Glu Ser Ser Asp Thr Ile Asp Asn Val Lys Ser Lys Ile Gln Asp 
            20                  25                  30          

Lys Glu 
        

<210>  34
<211>  42
<212>  PRT
<213>  S. cerevisiae

<400>  34

Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln Leu 
1               5                   10                  15      

Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser Thr 
            20                  25                  30          

Leu His Leu Val Leu Arg Leu Arg Gly Gly 
        35                  40          


<210>  35
<211>  5
<212>  PRT
<213>  artificial sequence

<220>
<223>  enterokinase recognition signal

<400>  35

Asp Asp Asp Asp Lys 
1               5   


<210>  36
<211>  216
<212>  PRT
<213>  artificial sequence

<220>
<223>  N-terminal sfGFP/C-terminal Fos

<400>  36

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      

Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly Glu 
            20                  25                  30          

Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              

Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  

Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg 
65                  70                  75                  80  

His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      

Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Val Val 
            100                 105                 110         

Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Thr 
        115                 120                 125             

Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 

Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Gly Gly Ser 
145                 150                 155                 160 

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ala 
                165                 170                 175     

Ser Arg Glu Leu Thr Asp Thr Leu Gln Ala Glu Thr Asp Gln Leu Glu 
            180                 185                 190         

Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala Asn Leu Leu Lys Glu 
        195                 200                 205             

Lys Glu Lys Leu Glu Gly Ala Pro 
    210                 215     


<210>  37
<211>  142
<212>  PRT
<213>  artificial sequence

<220>
<223>  N-terminal Fos/C-terminal sfGFP

<400>  37

Ser Gly Ala Ser Arg Glu Leu Thr Asp Thr Leu Gln Ala Glu Thr Asp 
1               5                   10                  15      

Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala Asn Leu 
            20                  25                  30          

Leu Lys Glu Lys Glu Lys Leu Glu Gly Ala Pro Gly Gly Ser Gly Gly 
        35                  40                  45              

Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Lys Asn Gly 
    50                  55                  60                  

Ile Lys Ala Asn Phe Thr Val Arg His Asn Val Glu Asp Gly Ser Val 
65                  70                  75                  80  

Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
                85                  90                  95      

Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Thr Val Leu Ser 
            100                 105                 110         

Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu His Glu Tyr Val 
        115                 120                 125             

Asn Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
    130                 135                 140         


<210>  38
<211>  288
<212>  PRT
<213>  artificial sequence

<220>
<223>  N-terminal Renilla/C-terminal Fos

<400>  38

Met Ala Ser Lys Val Tyr Asp Pro Glu Gln Arg Lys Arg Met Ile Thr 
1               5                   10                  15      

Gly Pro Gln Trp Trp Ala Arg Cys Lys Gln Met Asn Val Leu Asp Ser 
            20                  25                  30          

Phe Ile Asn Tyr Tyr Asp Ser Glu Lys His Ala Glu Asn Ala Val Ile 
        35                  40                  45              

Phe Leu His Gly Asn Ala Ala Ser Ser Tyr Leu Trp Arg His Val Val 
    50                  55                  60                  

Pro His Ile Glu Pro Val Ala Arg Cys Ile Ile Pro Asp Leu Ile Gly 
65                  70                  75                  80  

Met Gly Lys Ser Gly Lys Ser Gly Asn Gly Ser Tyr Arg Leu Leu Asp 
                85                  90                  95      

His Tyr Lys Tyr Leu Thr Ala Trp Phe Glu Leu Leu Asn Leu Pro Lys 
            100                 105                 110         

Lys Ile Ile Phe Val Gly His Asp Trp Gly Ala Cys Leu Ala Phe His 
        115                 120                 125             

Tyr Ser Tyr Glu His Gln Asp Lys Ile Lys Ala Ile Val His Ala Glu 
    130                 135                 140                 

Ser Val Val Asp Val Ile Glu Ser Trp Asp Glu Trp Pro Asp Ile Glu 
145                 150                 155                 160 

Glu Asp Ile Ala Leu Ile Lys Ser Glu Glu Gly Glu Lys Met Val Leu 
                165                 170                 175     

Glu Asn Asn Phe Phe Val Glu Thr Met Leu Pro Ser Lys Ile Met Arg 
            180                 185                 190         

Lys Leu Glu Pro Glu Glu Phe Ala Ala Tyr Leu Glu Pro Phe Lys Glu 
        195                 200                 205             

Lys Gly Glu Val Arg Arg Pro Thr Leu Ser Trp Pro Arg Glu Ile Pro 
    210                 215                 220                 

Leu Val Lys Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
225                 230                 235                 240 

Gly Gly Gly Ser Gly Gly Gly Ala Ser Arg Glu Leu Thr Asp Thr Leu 
                245                 250                 255     

Gln Ala Glu Thr Asp Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr 
            260                 265                 270         

Glu Ile Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Gly Ala Pro 
        275                 280                 285             


<210>  39
<211>  143
<212>  PRT
<213>  artificial sequence

<220>
<223>  N-terminal Fos/C-terminal Renilla

<400>  39

Ser Gly Ala Ser Arg Glu Leu Thr Asp Thr Leu Gln Ala Glu Thr Asp 
1               5                   10                  15      

Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala Asn Leu 
            20                  25                  30          

Leu Lys Glu Lys Glu Lys Leu Glu Gly Ala Pro Gly Gly Ser Gly Gly 
        35                  40                  45              

Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Lys Pro Asp 
    50                  55                  60                  

Val Val Gln Ile Val Arg Asn Tyr Asn Ala Tyr Leu Arg Ala Ser Asp 
65                  70                  75                  80  

Asp Leu Pro Lys Met Phe Ile Glu Ser Asp Pro Gly Phe Phe Ser Asn 
                85                  90                  95      

Ala Ile Val Glu Gly Ala Lys Lys Phe Pro Asn Thr Glu Phe Val Lys 
            100                 105                 110         

Val Lys Gly Leu His Phe Ser Gln Glu Asp Ala Pro Asp Glu Met Gly 
        115                 120                 125             

Lys Tyr Ile Lys Ser Phe Val Glu Arg Val Leu Lys Asn Glu Gln 
    130                 135                 140             


<210>  40
<211>  282
<212>  PRT
<213>  artificial sequence

<220>
<223>  N-terminal sfGFP/C-terminal mFKBP

<400>  40

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      

Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly Glu 
            20                  25                  30          

Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              

Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  

Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg 
65                  70                  75                  80  

His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      

Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Val Val 
            100                 105                 110         

Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Thr 
        115                 120                 125             

Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 

Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Gly Gly Ser 
145                 150                 155                 160 

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly 
                165                 170                 175     

Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys 
            180                 185                 190         

Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly 
        195                 200                 205             

Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met 
    210                 215                 220                 

Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln 
225                 230                 235                 240 

Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala 
                245                 250                 255     

Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr Leu 
            260                 265                 270         

Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
        275                 280         


<210>  41
<211>  207
<212>  PRT
<213>  artificial sequence

<220>
<223>  N-terminal mFKBP/C-terminal sfGFP

<400>  41

Met Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe 
1               5                   10                  15      

Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu 
            20                  25                  30          

Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys 
        35                  40                  45              

Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val 
    50                  55                  60                  

Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp 
65                  70                  75                  80  

Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala 
                85                  90                  95      

Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly Gly Ser Gly 
            100                 105                 110         

Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Lys Asn 
        115                 120                 125             

Gly Ile Lys Ala Asn Phe Thr Val Arg His Asn Val Glu Asp Gly Ser 
    130                 135                 140                 

Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
145                 150                 155                 160 

Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Thr Val Leu 
                165                 170                 175     

Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu His Glu Tyr 
            180                 185                 190         

Val Asn Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
        195                 200                 205         


<210>  42
<211>  354
<212>  PRT
<213>  artificial sequence

<220>
<223>  N-terminal Renilla/C-terminal mFKBP

<400>  42

Met Ala Ser Lys Val Tyr Asp Pro Glu Gln Arg Lys Arg Met Ile Thr 
1               5                   10                  15      

Gly Pro Gln Trp Trp Ala Arg Cys Lys Gln Met Asn Val Leu Asp Ser 
            20                  25                  30          

Phe Ile Asn Tyr Tyr Asp Ser Glu Lys His Ala Glu Asn Ala Val Ile 
        35                  40                  45              

Phe Leu His Gly Asn Ala Ala Ser Ser Tyr Leu Trp Arg His Val Val 
    50                  55                  60                  

Pro His Ile Glu Pro Val Ala Arg Cys Ile Ile Pro Asp Leu Ile Gly 
65                  70                  75                  80  

Met Gly Lys Ser Gly Lys Ser Gly Asn Gly Ser Tyr Arg Leu Leu Asp 
                85                  90                  95      

His Tyr Lys Tyr Leu Thr Ala Trp Phe Glu Leu Leu Asn Leu Pro Lys 
            100                 105                 110         

Lys Ile Ile Phe Val Gly His Asp Trp Gly Ala Cys Leu Ala Phe His 
        115                 120                 125             

Tyr Ser Tyr Glu His Gln Asp Lys Ile Lys Ala Ile Val His Ala Glu 
    130                 135                 140                 

Ser Val Val Asp Val Ile Glu Ser Trp Asp Glu Trp Pro Asp Ile Glu 
145                 150                 155                 160 

Glu Asp Ile Ala Leu Ile Lys Ser Glu Glu Gly Glu Lys Met Val Leu 
                165                 170                 175     

Glu Asn Asn Phe Phe Val Glu Thr Met Leu Pro Ser Lys Ile Met Arg 
            180                 185                 190         

Lys Leu Glu Pro Glu Glu Phe Ala Ala Tyr Leu Glu Pro Phe Lys Glu 
        195                 200                 205             

Lys Gly Glu Val Arg Arg Pro Thr Leu Ser Trp Pro Arg Glu Ile Pro 
    210                 215                 220                 

Leu Val Lys Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
225                 230                 235                 240 

Gly Gly Gly Ser Gly Gly Gly Gly Val Gln Val Glu Thr Ile Ser Pro 
                245                 250                 255     

Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His 
            260                 265                 270         

Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp 
        275                 280                 285             

Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg 
    290                 295                 300                 

Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys 
305                 310                 315                 320 

Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly 
                325                 330                 335     

Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys 
            340                 345                 350         

Leu Glu 
        

<210>  43
<211>  208
<212>  PRT
<213>  artificial sequence

<220>
<223>  N-terminal mFKBP/C-terminal Renilla

<400>  43

Met Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe 
1               5                   10                  15      

Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu 
            20                  25                  30          

Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys 
        35                  40                  45              

Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val 
    50                  55                  60                  

Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp 
65                  70                  75                  80  

Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala 
                85                  90                  95      

Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly Gly Ser Gly 
            100                 105                 110         

Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Lys Pro 
        115                 120                 125             

Asp Val Val Gln Ile Val Arg Asn Tyr Asn Ala Tyr Leu Arg Ala Ser 
    130                 135                 140                 

Asp Asp Leu Pro Lys Met Phe Ile Glu Ser Asp Pro Gly Phe Phe Ser 
145                 150                 155                 160 

Asn Ala Ile Val Glu Gly Ala Lys Lys Phe Pro Asn Thr Glu Phe Val 
                165                 170                 175     

Lys Val Lys Gly Leu His Phe Ser Gln Glu Asp Ala Pro Asp Glu Met 
            180                 185                 190         

Gly Lys Tyr Ile Lys Ser Phe Val Glu Arg Val Leu Lys Asn Glu Gln 
        195                 200                 205             


<210>  44
<211>  16
<212>  DNA
<213>  artificial sequence

<220>
<223>  conjugate

<400>  44
gtccagatgt ctttgc                                                  16


<210>  45
<211>  17
<212>  DNA
<213>  artificial sequence

<220>
<223>  conjugate

<400>  45
gctgtgtcct gaagaaa                                                 17


<210>  46
<211>  17
<212>  DNA
<213>  artificial sequence

<220>
<223>  conjugate

<400>  46
tttcttcagg acacagc                                                 17


<210>  47
<211>  221
<212>  PRT
<213>  artificial sequence

<220>
<223>  N-terminal Gaussia split-protein fragment-FKBP single F36V mutant
       fusion protein

<400>  47

Met Lys Pro Thr Glu Asn Asn Glu Asp Phe Asn Ile Val Ala Val Ala 
1               5                   10                  15      

Ser Asn Phe Ala Thr Thr Asp Leu Asp Ala Asp Arg Gly Lys Leu Pro 
            20                  25                  30          

Gly Lys Lys Leu Pro Leu Glu Val Leu Lys Glu Met Glu Ala Asn Ala 
        35                  40                  45              

Arg Lys Ala Gly Cys Thr Arg Gly Cys Leu Ile Cys Leu Ser His Ile 
    50                  55                  60                  

Lys Cys Thr Pro Lys Met Lys Lys Phe Ile Pro Gly Arg Cys His Thr 
65                  70                  75                  80  

Tyr Glu Gly Asp Lys Glu Ser Ala Gln Gly Gly Ile Gly Gly Ser Gly 
                85                  90                  95      

Gly Gly Gly Ser Ser Gly Gly Gly Gly Val Gln Val Glu Thr Ile Ser 
            100                 105                 110         

Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val 
        115                 120                 125             

His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg 
    130                 135                 140                 

Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile 
145                 150                 155                 160 

Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala 
                165                 170                 175     

Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro 
            180                 185                 190         

Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu 
        195                 200                 205             

Lys Leu Glu Gly Gly Ser Gly His His His His His His 
    210                 215                 220     


<210>  48
<211>  221
<212>  PRT
<213>  artificial sequence

<220>
<223>  N-terminal Gaussia split-protein fragment-FKBP C22S / F36V double
       mutant fusion protein

<400>  48

Met Lys Pro Thr Glu Asn Asn Glu Asp Phe Asn Ile Val Ala Val Ala 
1               5                   10                  15      

Ser Asn Phe Ala Thr Thr Asp Leu Asp Ala Asp Arg Gly Lys Leu Pro 
            20                  25                  30          

Gly Lys Lys Leu Pro Leu Glu Val Leu Lys Glu Met Glu Ala Asn Ala 
        35                  40                  45              

Arg Lys Ala Gly Cys Thr Arg Gly Cys Leu Ile Cys Leu Ser His Ile 
    50                  55                  60                  

Lys Cys Thr Pro Lys Met Lys Lys Phe Ile Pro Gly Arg Cys His Thr 
65                  70                  75                  80  

Tyr Glu Gly Asp Lys Glu Ser Ala Gln Gly Gly Ile Gly Gly Ser Gly 
                85                  90                  95      

Gly Gly Gly Ser Ser Gly Gly Gly Gly Val Gln Val Glu Thr Ile Ser 
            100                 105                 110         

Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Ser Val Val 
        115                 120                 125             

His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg 
    130                 135                 140                 

Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile 
145                 150                 155                 160 

Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala 
                165                 170                 175     

Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro 
            180                 185                 190         

Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu 
        195                 200                 205             

Lys Leu Glu Gly Gly Ser Gly His His His His His His 
    210                 215                 220     


<210>  49
<211>  205
<212>  PRT
<213>  artificial sequence

<220>
<223>  FKBP-C-terminal Gaussia split-protein fragment single FKBP F36V 
       mutant fusion protein

<400>  49

Met His His His His His His Gly Gly Ser Gly Gly Val Gln Val Glu 
1               5                   10                  15      

Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr 
            20                  25                  30          

Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp 
        35                  40                  45              

Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln 
    50                  55                  60                  

Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly 
65                  70                  75                  80  

Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr 
                85                  90                  95      

Gly His Pro Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val 
            100                 105                 110         

Glu Leu Leu Lys Leu Glu Gly Ser Gly Gly Gly Gly Ser Ser Gly Gly 
        115                 120                 125             

Gly Glu Ala Ile Val Asp Ile Pro Glu Ile Pro Gly Phe Lys Asp Leu 
    130                 135                 140                 

Glu Pro Met Glu Gln Phe Ile Ala Gln Val Asp Leu Cys Val Asp Cys 
145                 150                 155                 160 

Thr Thr Gly Cys Leu Lys Gly Leu Ala Asn Val Gln Cys Ser Asp Leu 
                165                 170                 175     

Leu Lys Lys Trp Leu Pro Gln Arg Cys Ala Thr Phe Ala Ser Lys Ile 
            180                 185                 190         

Gln Gly Gln Val Asp Lys Ile Lys Gly Ala Gly Gly Asp 
        195                 200                 205 


<210>  50
<211>  205
<212>  PRT
<213>  artificial sequence

<220>
<223>  sequence of the FKBP-C-terminal Gaussia split-protein fragment 
       double FKBP C22S / F36V mutant fusion protein

<400>  50

Met His His His His His His Gly Gly Ser Gly Gly Val Gln Val Glu 
1               5                   10                  15      

Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr 
            20                  25                  30          

Ser Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp 
        35                  40                  45              

Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln 
    50                  55                  60                  

Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly 
65                  70                  75                  80  

Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr 
                85                  90                  95      

Gly His Pro Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val 
            100                 105                 110         

Glu Leu Leu Lys Leu Glu Gly Ser Gly Gly Gly Gly Ser Ser Gly Gly 
        115                 120                 125             

Gly Glu Ala Ile Val Asp Ile Pro Glu Ile Pro Gly Phe Lys Asp Leu 
    130                 135                 140                 

Glu Pro Met Glu Gln Phe Ile Ala Gln Val Asp Leu Cys Val Asp Cys 
145                 150                 155                 160 

Thr Thr Gly Cys Leu Lys Gly Leu Ala Asn Val Gln Cys Ser Asp Leu 
                165                 170                 175     

Leu Lys Lys Trp Leu Pro Gln Arg Cys Ala Thr Phe Ala Ser Lys Ile 
            180                 185                 190         

Gln Gly Gln Val Asp Lys Ile Lys Gly Ala Gly Gly Asp 
        195                 200                 205 


<210>  51
<211>  188
<212>  PRT
<213>  artificial sequence

<220>
<223>  sequence of the wild-type FRB-C-terminal Gaussia split-protein 
       fragment mutant C61S mutant fusion protein

<400>  51

Met His His His His His His Gly Gly Ser Gly Glu Met Trp His Glu 
1               5                   10                  15      

Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys 
            20                  25                  30          

Gly Met Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly 
        35                  40                  45              

Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp 
    50                  55                  60                  

Leu Met Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn 
65                  70                  75                  80  

Val Lys Asp Leu Thr Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg 
                85                  90                  95      

Arg Ile Ser Lys Gln Gly Ser Gly Gly Gly Gly Ser Ser Gly Gly Gly 
            100                 105                 110         

Glu Ala Ile Val Asp Ile Pro Glu Ile Pro Gly Phe Lys Asp Leu Glu 
        115                 120                 125             

Pro Met Glu Gln Phe Ile Ala Gln Val Asp Leu Cys Val Asp Cys Thr 
    130                 135                 140                 

Thr Gly Cys Leu Lys Gly Leu Ala Asn Val Gln Cys Ser Asp Leu Leu 
145                 150                 155                 160 

Lys Lys Trp Leu Pro Gln Arg Cys Ala Thr Phe Ala Ser Lys Ile Gln 
                165                 170                 175     

Gly Gln Val Asp Lys Ile Lys Gly Ala Gly Gly Asp 
            180                 185             


<210>  52
<211>  188
<212>  PRT
<213>  artificial sequence

<220>
<223>  sequence of the C61S mutant FRB-C-terminal Gaussia split-protein 
       fragment fusion protein

<400>  52

Met His His His His His His Gly Gly Ser Gly Glu Met Trp His Glu 
1               5                   10                  15      

Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys 
            20                  25                  30          

Gly Met Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly 
        35                  40                  45              

Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp 
    50                  55                  60                  

Leu Met Glu Ala Gln Glu Trp Ser Arg Lys Tyr Met Lys Ser Gly Asn 
65                  70                  75                  80  

Val Lys Asp Leu Thr Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg 
                85                  90                  95      

Arg Ile Ser Lys Gln Gly Ser Gly Gly Gly Gly Ser Ser Gly Gly Gly 
            100                 105                 110         

Glu Ala Ile Val Asp Ile Pro Glu Ile Pro Gly Phe Lys Asp Leu Glu 
        115                 120                 125             

Pro Met Glu Gln Phe Ile Ala Gln Val Asp Leu Cys Val Asp Cys Thr 
    130                 135                 140                 

Thr Gly Cys Leu Lys Gly Leu Ala Asn Val Gln Cys Ser Asp Leu Leu 
145                 150                 155                 160 

Lys Lys Trp Leu Pro Gln Arg Cys Ala Thr Phe Ala Ser Lys Ile Gln 
                165                 170                 175     

Gly Gln Val Asp Lys Ile Lys Gly Ala Gly Gly Asp 
            180                 185             


<210>  53
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  control oligonucleotide

<400>  53
agctgtgtcc tgaagaaagc aaagacatct ggaca                             35


<210>  54
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  scrambled control oligonucleotide

<400>  54
gactagacgg ccagggagac gaatacatat tcaat                             35
