                         SEQUENCE LISTING

<110>  The Pennsylvania State University
 
<120>  DEVICES AND METHODS FOR GENERATING ELECTRICAL CURRENT FROM 
       METHANE

<130>  36PST86452WO

<150>  62/425,421
<151>  2016-11-22

<150>   62/445,871
<151>  2017-01-13

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  579
<212>  PRT
<213>  Anaerobic methanotrophic archaeal population 1 (ANME-1) from Black Sea mat

<400>  1

Met Pro Tyr Asn Asp Ile Gln His Asn Phe Leu Lys Ala Met Ser Asp 
1               5                   10                  15      


Lys Phe Ala Glu Lys Pro Glu Ser Thr Ala Thr Glu Phe Tyr Thr Tyr 
            20                  25                  30          


Gly Gly Ile Ala Gln Lys Gly Gly Met Arg Lys Arg Glu Phe Ile Ala 
        35                  40                  45              


Glu Ala Ser Lys Ile Val Asp Ser Arg Val Asn Ser Thr Pro Ala Tyr 
    50                  55                  60                  


Asn Pro Asp Ala Gly Met Pro Gln Gly Gln Arg Tyr Leu Met Pro Tyr 
65                  70                  75                  80  


Met Met Asn His Thr Asp Ile Met Val Asn Ala Asp Asp Leu His Trp 
                85                  90                  95      


Ile Asn Asn Ala Ala Met Gln Gln Ala Trp Asp Asp Met Lys Arg Gly 
            100                 105                 110         


Ile Val Leu Gly Leu Asp Asp Ala His Gly Leu Leu Glu Ala Arg Leu 
        115                 120                 125             


Gly Lys Glu Val Thr Pro Asp Thr Ile Ser Asn Tyr Met Glu Val Leu 
    130                 135                 140                 


Asn His Ala Leu Pro Gly Gly Ala Val Ile Gln Glu His Met Val Glu 
145                 150                 155                 160 


Thr Lys Pro Met Leu Val Asn Asp Ser Tyr Ala Lys Ile Phe Ser Gly 
                165                 170                 175     


Asp Asp Asp Leu Val Asp Ser Val Asp Arg Arg Phe Ile Leu Asp Ile 
            180                 185                 190         


Asn Lys Glu Phe Ala Ala Gly Tyr Asp Lys Pro Gly Glu Gln Ala Asp 
        195                 200                 205             


Gln Leu Lys Asp Ala Ile Gly Lys Lys Ile Trp Gln Ile Leu Trp Met 
    210                 215                 220                 


Pro Thr Val Val Ala Arg Gln Thr Asp Gly Gly Thr Met Phe Arg Trp 
225                 230                 235                 240 


Val Gly Met Gln Val Gly Met Thr Met Ile Asn Ala Tyr Lys Leu Cys 
                245                 250                 255     


Ala Gly Glu Ser Val Thr Gly Glu Phe Ala Tyr Tyr Ala Lys His Ala 
            260                 265                 270         


Ala Val Val Gln Leu Ser Asn Tyr Met Pro Val Lys Arg Ala Arg Ser 
        275                 280                 285             


His Asn Glu Pro Gly Gly Met Pro Leu Gly Ile Asn Ala Asp Ser Thr 
    290                 295                 300                 


Arg Ser Pro Ala Leu Phe Pro Asn Asp Pro Ile Arg Ala Glu Leu Glu 
305                 310                 315                 320 


Ser Ile Ala Val Ala Ala Met Val Tyr Asp Gln Leu Trp Phe Gly Thr 
                325                 330                 335     


Tyr Met Ser Gly Gly Val Gly Phe Thr Gln Tyr Ala Ser Ala Thr Tyr 
            340                 345                 350         


Thr Asp Asn Ile Leu Glu Asp Phe Cys Tyr Lys Gly Cys Glu Ile Gly 
        355                 360                 365             


Leu Asp Tyr Ala Gly Gly Lys Met Ala Ser Ile Lys Gly Asp Lys Leu 
    370                 375                 380                 


Asn Met Asp Ile Leu Glu Glu Ile Ile Arg Ala Glu Asn Asp Tyr Ala 
385                 390                 395                 400 


Leu Thr Gln Tyr Glu Ala Tyr Pro Thr Val Ala Glu Ser His Phe Gly 
                405                 410                 415     


Gly Ser Val Arg Ala Cys Cys Ala Ala Ala Gly Cys Gly Ser Ala Val 
            420                 425                 430         


Ala Cys Ala Thr Gly Leu Ala Gln Pro Ala Leu Ser Ala Trp Ser Leu 
        435                 440                 445             


Ser Met Leu Gly His Tyr Glu Arg Val Gly Arg Leu Gly Phe Phe Gly 
    450                 455                 460                 


Tyr Asp Leu Gln Asp Gln Cys Thr Ala Cys Gly Ser Tyr Ser Tyr Gln 
465                 470                 475                 480 


Ser Asp Glu Gly Met Pro Phe Glu Met Arg Gly Val Asn Tyr Pro Asn 
                485                 490                 495     


Tyr Ala Met Asn Val Gly His Gln Ser Ala Tyr Ala Gly Leu Val Ala 
            500                 505                 510         


Gly Ala His Ser Ala Asn His Asp Ala Trp Val Leu Ser Pro Leu Trp 
        515                 520                 525             


Lys Val Ala Phe Ser Asp Arg Asp Leu Pro Phe Asp Arg Gly Tyr Val 
    530                 535                 540                 


Thr Arg Glu Tyr Gly Leu Gly Ala Asn Arg Glu Tyr Thr Lys Val Ala 
545                 550                 555                 560 


Gly Glu Arg Asp Leu Ile Ile Ala Gly His Tyr Gly Arg Glu Pro Gly 
                565                 570                 575     


Ala Lys Leu 
            


<210>  2
<211>  1740
<212>  DNA
<213>  Anaerobic methanotrophic archaeal population 1 (ANME-1) from Black Sea mat

<400>  2
atgccatata atgatataca gcacaatttc ctgaaagcga tgtcagataa gttcgcggag       60

aagccagaga gtacagcgac cgagttctat acgtacggcg gtatagcgca gaaaggagga      120

atgagaaaga gggagttcat agcggaagcg tcgaagatag tagatagccg agtcaactca      180

acgccagcat acaatccgga tgcgggtatg ccacaagggc agagatacct gatgccatac      240

atgatgaacc acacggacat catggtaaac gcagatgacc tgcactggat caacaatgcc      300

gcgatgcagc aggcctggga tgatatgaag agaggaattg tcctgggact ggacgatgca      360

cacggactgt tagaagcgag attgggcaag gaagttacgc cagatacaat aagcaactac      420

atggaagtgt tgaaccacgc gctgccgggt ggtgcggtaa ttcaggagca catggtagag      480

acgaagccga tgctggtgaa tgacagctat gccaagatat tctccggaga tgacgacctt      540

gtagattctg ttgacaggag attcattctg gacataaaca aggaattcgc agcaggctat      600

gataaaccgg gcgagcaggc cgaccagttg aaagatgcaa ttggcaagaa gatctggcag      660

atcctctgga tgccgactgt tgtagcacga cagacagatg gaggtacgat gttcagatgg      720

gtaggtatgc aggtcggaat gacaatgata aacgcataca agctgtgtgc aggagaatca      780

gtcacgggtg agttcgcata ctacgcgaag cacgctgctg tggttcagtt gtcgaactac      840

atgccagtaa agagagcaag gtcacacaat gaacctggtg gtatgccatt aggtataaac      900

gcagacagta cacggtcacc agcgttgttc ccgaacgacc cgataagggc tgagctggag      960

agtatcgcag ttgcggctat ggtctatgac cagttgtggt tcggaacgta catgtcaggt     1020

ggtgtaggat tcacgcagta cgcaagtgcg acctacacgg acaacatcct ggaggacttc     1080

tgctacaagg gctgcgaaat cggactggat tacgcaggcg gcaagatggc ttcgataaag     1140

ggcgacaagc tcaacatgga catcctggag gagataataa gagcagagaa cgattatgca     1200

ctgacgcagt atgaagcata tccaacagta gcggaatctc acttcggtgg atctgttaga     1260

gcgtgctgtg cagcagcggg atgtggtagt gcagttgcat gcgcaacagg acttgcacaa     1320

cctgcattga gtgcgtggtc actgtctatg ctgggacact atgagcgtgt tggaagactc     1380

ggattcttcg ggtacgactt gcaagatcag tgtacggcat gcggctcgta ttcataccag     1440

agtgatgagg gaatgccatt tgagatgcgc ggtgtgaact atccgaacta cgcgatgaac     1500

gtcgggcacc agagtgcgta cgcaggtcta gttgctggag cacactctgc aaatcatgac     1560

gcatgggtgc tgtcaccgtt gtggaaagta gcgttctcgg accgtgacct accgttcgac     1620

cgtggttacg tgaccagaga gtacggactg ggtgcaaaca gagaatacac caaggtcgcc     1680

ggagagagag atctgattat agcaggtcac tacggcagag aacccggtgc aaaactgtaa     1740


<210>  3
<211>  433
<212>  PRT
<213>  Anaerobic methanotrophic archaeal population 1 (ANME-1) from Black Sea mat

<400>  3

Met Ala Asp Glu Ile Asp Leu Tyr Asp Asp Lys Gly Lys Lys Leu Ala 
1               5                   10                  15      


Ala Gly Val Pro Leu Gln Asn Ile Ser Pro Leu Lys Asn Ala Ala Ile 
            20                  25                  30          


Lys Lys Ile Val Asn Leu Thr Ile Arg Thr Gly Ala Val Asp Leu Ala 
        35                  40                  45              


Gly Leu Glu Lys Lys Phe Ala Thr Gly Ala Ile Ala Gly Arg Gly Met 
    50                  55                  60                  


Val Ile Arg Gly Val Asn Arg Asn Leu Pro Ile Val Asp Lys Ala Lys 
65                  70                  75                  80  


Glu Ile Ala Lys Ala Val Glu Asp Met Leu Arg Val Glu Ser Gly Asp 
                85                  90                  95      


Asp Thr Asn Val Glu Leu Ile Ala Gly Gly Lys Arg Met Met Val Gln 
            100                 105                 110         


Pro Pro Thr Ala Arg Ile Leu Ser Asp Tyr Ser Val Gly Leu Thr Ala 
        115                 120                 125             


Ser Met Gly Ala Leu Thr His Ala Ile Ile Asp Val Cys Asn Val Ser 
    130                 135                 140                 


Met Trp Asp Ala Pro Tyr Val His Ala Gly Val Trp Gly Met Tyr Pro 
145                 150                 155                 160 


Gln Asn Pro Asp Pro Gly Asp Gly Ala Val Lys Met Leu Val Asp Ile 
                165                 170                 175     


Pro Met Lys Asn Glu Gly Pro Gly Phe Thr Leu Arg Asn Ile Pro Val 
            180                 185                 190         


Asn His Leu Ala Ala Thr Val Arg Lys Arg Ala Met Gln Gly Ala Gly 
        195                 200                 205             


Leu Thr Met Ile Leu Glu Glu Ala Ala Gln Phe Glu Met Gly Asn Cys 
    210                 215                 220                 


Met Gly Pro His Glu Arg Gly His Leu Leu Asp Leu Ala Tyr Glu Gly 
225                 230                 235                 240 


Leu Asn Ala Asn Asn Leu Leu Tyr Ser Leu Ile Lys Asp Asn Gly Gln 
                245                 250                 255     


Asp Gly Ser Leu Gly Asp Val Ile Tyr Ala Ala Val Glu Lys Ala Lys 
            260                 265                 270         


Ala Asp Gly Val Ile Lys Ser Leu Lys Lys Met Pro Ser Gly Phe Thr 
        275                 280                 285             


Val Tyr Asp Ala Asp Asp Met Gln Leu Trp Asn Ala Tyr Ala Cys Thr 
    290                 295                 300                 


Ala Met Leu Ala Gly Val Cys Val Asn Cys Ala Ser Met Arg Ala Gly 
305                 310                 315                 320 


Gln Pro Val Pro Gly Asn Ile Met Gln Ala Cys Cys Leu Ile Glu Arg 
                325                 330                 335     


Glu Thr Gly Leu Pro Gly Pro Asp Phe Gly Met Ala Gln Gly Ala Ser 
            340                 345                 350         


Val Ser Ser Ser Phe Phe Ser His Ser Ile Tyr Gly Gly Gly Gly Pro 
        355                 360                 365             


Gly Val Phe Tyr Gly Asn His Ile Val Thr Arg His Ala Lys Gly Gln 
    370                 375                 380                 


Phe Ile Pro Cys Phe Cys Ala Ala Met Cys Ile Asp Ala Asp Thr Met 
385                 390                 395                 400 


Tyr Phe Ser Pro Ala Arg Thr Ser Ala Leu Tyr Gly Glu Val Leu Gly 
                405                 410                 415     


Ala Ile Pro Glu Phe Ala Glu Pro Met Arg Ala Val Ala Glu Ala Ala 
            420                 425                 430         


Lys 
    


<210>  4
<211>  1302
<212>  DNA
<213>  Anaerobic methanotrophic archaeal population 1 (ANME-1) from Black Sea mat

<400>  4
atggcagatg aaatagattt atatgacgac aaaggcaaga agttagcggc tggtgtacca       60

ttacagaaca tcagtccgct gaagaatgca gcgataaaga agatagttaa cctgaccatc      120

aggacaggtg ctgttgactt agcaggtctg gagaagaagt tcgcaaccgg cgcaattgcg      180

ggtagaggta tggtaatccg gggcgtaaat aggaacttac cgatagtgga caaagcgaaa      240

gagatagcga aagcagtaga ggacatgttg cgcgttgagt caggagacga cacaaacgta      300

gaattaatcg caggtggaaa gcgaatgatg gtgcagccac caaccgcgag aatactatcc      360

gattattcgg ttggtctgac cgcgtcaatg ggtgcgctaa cgcatgcgat tattgacgtc      420

tgcaacgtga gtatgtggga tgcaccgtat gtgcatgctg gtgtgtgggg tatgtaccca      480

caaaatccgg atcccggtga tggggcagta aagatgctcg tggatatacc gatgaagaat      540

gagggaccgg gtttcacgct gaggaacata cctgtgaacc acttagcagc gacagtgagg      600

aagagagcga tgcaaggtgc gggcctgacc atgattctgg aagaggcagc gcagttcgag      660

atgggtaact gtatgggacc gcatgagcgt ggtcaccttc tggatcttgc gtatgaagga      720

ctgaatgcga acaacctgtt gtatagcctg ataaaagaca acgggcagga cgggtcactt      780

ggcgatgtga tatacgctgc ggtggagaaa gcgaaagcag acggtgtgat aaagtcactg      840

aagaagatgc catccggatt cacagtgtat gacgcggatg acatgcagtt gtggaacgca      900

tacgcatgca cggctatgct tgcgggtgta tgtgtgaact gcgcatcaat gcgtgcaggt      960

cagccagtac cgggtaacat tatgcaagcc tgctgtctga tagagagaga gacaggactg     1020

cctggaccag actttggaat ggctcagggt gcgtcagtat cgagctcgtt cttctcgcac     1080

tccatatatg gaggcggtgg accgggtgtg ttctatggga accacatcgt gacgaggcat     1140

gcgaagggtc agttcatacc ctgtttctgt gctgcgatgt gcatagatgc ggacacgatg     1200

tatttctcac cagcgaggac gtcagcattg tatggggaag tattgggtgc aataccagaa     1260

tttgcagaac caatgagggc agttgcggag gctgccaagt aa                        1302


<210>  5
<211>  279
<212>  PRT
<213>  Anaerobic methanotrophic archaeal population 1 (ANME-1) from Black Sea mat

<400>  5

Met Pro Gln Phe Thr Ala Gly Asn Ser His Val Ala Gln Asn Arg Arg 
1               5                   10                  15      


Asn Tyr Met Asp Pro Ser Tyr Lys Leu Glu Lys Leu Arg Asp Ile Pro 
            20                  25                  30          


Glu Glu Asp Ile Val Arg Leu Leu Ala His Arg Ala Pro Gly Glu Glu 
        35                  40                  45              


Tyr Lys Ser Ile His Pro Pro Leu Glu Glu Met Glu Glu Pro Asp Cys 
    50                  55                  60                  


Ala Val Arg Gln Ile Val Lys Pro Thr Glu Gly Ala Ala Ala Gly Asp 
65                  70                  75                  80  


Arg Ile Arg Tyr Val Gln Tyr Thr Asp Ser Met Phe Phe Ser Pro Ile 
                85                  90                  95      


Thr Pro Tyr Gln Arg Ala Trp Glu Ala Leu Asn Arg Tyr Lys Gly Val 
            100                 105                 110         


Asp Pro Gly Val Leu Ser Gly Arg Thr Ile Ile Glu Ala Arg Glu Arg 
        115                 120                 125             


Asp Ile Glu Lys Ile Ala Lys Ile Glu Val Asp Cys Glu Leu Tyr Asp 
    130                 135                 140                 


Thr Ala Arg Thr Gly Leu Arg Gly Arg Thr Val His Gly His Ala Val 
145                 150                 155                 160 


Arg Leu Asp Lys Asp Gly Met Met Phe Asp Ala Leu Arg Arg Trp Ser 
                165                 170                 175     


Arg Gly Ala Asp Gly Thr Val Thr Tyr Val Lys Asp Met Ile Gly Gly 
            180                 185                 190         


Ala Met Asp Lys Glu Val Thr Leu Gly Lys Pro Leu Ser Asp Ala Glu 
        195                 200                 205             


Leu Leu Lys Lys Thr Thr Met Tyr Arg Asn Ala Gln Gly Gly Val Trp 
    210                 215                 220                 


Gln Glu Ala Asp Asp Pro Glu Ser Met Asp Val Thr Ala Gln Ile His 
225                 230                 235                 240 


Trp Lys Arg Ser Val Gly Gly Phe Gln Pro Trp Ala Lys Met Lys Asp 
                245                 250                 255     


Ile Lys Gly Gly Lys Lys Asp Val Gly Val Lys Asn Leu Lys Leu Phe 
            260                 265                 270         


Thr Pro Arg Gly Gly Val Glu 
        275                 


<210>  6
<211>  840
<212>  DNA
<213>  Anaerobic methanotrophic archaeal population 1 (ANME-1) from Black Sea mat

<400>  6
atgccacaat ttacagcggg aaatagccat gtagcacaaa acagaagaaa ctacatggac       60

ccaagctaca aattagagaa actgagagac ataccagaag aagacattgt gagattgctt      120

gcgcaccgtg caccgggaga ggagtacaag agcatccatc caccattgga agagatggaa      180

gagccggact gcgcggttcg tcagatagtg aagccaacag aaggcgcagc agcaggtgac      240

aggatacgat acgtgcagta caccgattcg atgttcttct caccgataac gccttaccag      300

cgtgcatggg aagcattgaa ccgatataag ggtgtggacc caggagtgct gtcaggaagg      360

acgatcatag aagcacgaga gcgagatata gagaagatag cgaaaatcga ggttgattgc      420

gagctatacg atacagcaag aaccggcctg agaggaagga cagtgcacgg gcacgcagtc      480

cgactggaca aggacggtat gatgttcgat gcactacgaa gatggtcaag aggtgcagat      540

ggaacagtaa cctacgtgaa ggatatgatc ggcggtgcaa tggacaagga agtaacgtta      600

ggaaagccac tgtctgatgc agagctgctg aagaaaacga ctatgtacag gaacgcacag      660

ggcggagtct ggcaagaagc ggatgatccc gagtcaatgg acgtaactgc tcagatacac      720

tggaaaaggt cagtaggtgg attccaacca tgggcaaaga tgaaggacat aaaaggtgga      780

aagaaggacg taggcgtgaa gaacttgaaa ttatttacac caagaggagg agtggaataa      840


<210>  7
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer for amplifying v3-v4 hypervariable region of 16S 
       rDNA of prokaryotes


<220>
<221>  misc_feature
<222>  (42)..(42)
<223>  n is a, c, g, or t

<400>  7
tcgtcggcag cgtcagatgt gtataagaga cagcctacgg gnbgcascag                  50


<210>  8
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' adapter

<400>  8
tcgtcggcag cgtcagatgt gtataagaga cag                                    33


<210>  9
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer for amplifying v3-v4 hypervariable region of 16S 
       rDNA of prokaryotes


<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  n is a, c, g, or t

<400>  9
gtctcgtggg ctcggagatg tgtataagag acaggactac nvgggtatct aatcc            55


<210>  10
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' adapter

<400>  10
gtctcgtggg ctcggagatg tgtataagag acag                                   34


