﻿               SEQUENCE LISTING

<110> Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.

<120> Bacterial Glutaminyl Cyclases and Inhibitors Thereof for Use in the Treatment of Periodontitis

<130> 201 886

<150> EP16201913
<151> 2016-12-02

<160> 14

<170> BiSSAP 1.3.6

<210> 1
<211> 333
<212> PRT
<213> Porphyromonas gingivalis


<220> 
<223> PgQC

<400> 1
Met Lys Arg Leu Ile Thr Thr Gly Ala Ala Phe Leu Leu Ala Ala Thr 
1               5                   10                  15      
Leu Ser Ala Cys Asn Gly Asn Asn Thr Ser Glu Thr Gln Gly Asp Arg 
            20                  25                  30          
Thr Glu Gln Ala Glu Thr Val Gln Ala Asp Leu Phe Ser Ala Asp Ser 
        35                  40                  45              
Ala Tyr Thr Phe Val Gln Arg Gln Val Asn Phe Gly Pro Arg Ile Pro 
    50                  55                  60                  
Gly Thr Ala Pro His Arg Ala Cys Gly Asp Trp Leu Val Ala Thr Leu 
65                  70                  75                  80  
Arg Ser Phe Gly Ala Ala Val Gln Glu Gln Thr Ala Glu Ile Lys Ala 
                85                  90                  95      
His Asp Gly Thr Met Leu Pro Met Arg Asn Ile Ile Ala Ser Tyr Arg 
            100                 105                 110         
Pro Glu Ala Thr Gly Arg Met Leu Leu Met Ala His Trp Asp Thr Arg 
        115                 120                 125             
Pro Val Cys Asp Gln Asp Ala Asn Pro Ala Met His Thr Glu Thr Phe 
    130                 135                 140                 
Asp Gly Ala Asp Asp Gly Gly Ser Gly Val Gly Val Leu Leu Glu Ile 
145                 150                 155                 160 
Ala Arg Tyr Leu Gly Gln Gln Lys Asp Leu Gly Met Gly Ile Asp Ile 
                165                 170                 175     
Val Phe Phe Asp Thr Glu Asp Tyr Gly Ser Tyr Gly Asp Asp Glu Ser 
            180                 185                 190         
Trp Cys Leu Gly Ser Gln Tyr Trp Ser Arg Asn Pro His Val Ala Gly 
        195                 200                 205             
Tyr Lys Ala Glu Ala Gly Ile Leu Leu Asp Met Val Gly Ala Lys Gly 
    210                 215                 220                 
Ala Thr Phe Tyr Trp Glu Tyr Phe Ser Lys Ser Tyr Ala Pro Gly Leu 
225                 230                 235                 240 
Ile Ser Ala Val Trp Gln Thr Ala Ala Ala Leu Gly Tyr Gly Asn Tyr 
                245                 250                 255     
Phe Ile Gln Ala Asp Gly Gly Ala Leu Thr Asp Asp His Val Pro Val 
            260                 265                 270         
Ile Lys Asn Leu Gly Ile Pro Cys Ile Asp Ile Ile Asn Tyr Ser Ser 
        275                 280                 285             
Lys Asn Glu His Gly Phe Gly Asp His Trp His Thr Gln Arg Asp Asn 
    290                 295                 300                 
Met Gln Ile Ile Asp Lys Asn Val Leu Asp Ala Val Gly Glu Thr Val 
305                 310                 315                 320 
Ile Arg Tyr Leu Asp Glu Gln Val Lys Ala Ala Ser His 
                325                 330             

<210> 2
<211> 363
<212> PRT
<213> Prevotella intermedia


<220> 
<223> PiQC

<400> 2
Met Gly Arg Gln Leu Ala Ala Arg Tyr Gly Thr Asp Thr Gly Cys Gln 
1               5                   10                  15      
Thr Lys Ile Lys Arg Thr Thr Met Asn Gly Lys Ile Lys Phe Leu Cys 
            20                  25                  30          
Ser Gly Met Ala Val Leu Leu Leu Ala Ala Phe Ala Phe Ser Cys Lys 
        35                  40                  45              
Gly Lys Ser Ser Asn Asn Ser Thr Glu Asp Gly Asp Thr Val Ala Thr 
    50                  55                  60                  
Ala Lys Pro Val Gly Pro Thr Phe Asn Pro Asp Ser Ala Phe Ala Tyr 
65                  70                  75                  80  
Thr Ala Ala Gln Cys Asp Phe Gly Pro Arg Thr Met Asn Ser Ser Ala 
                85                  90                  95      
His Asp Lys Cys Glu Gln Trp Ile Ile Ser Lys Phe Lys Gln Tyr Gly 
            100                 105                 110         
Cys Glu Val Gln Thr Gln Lys Ala Asp Leu Lys Ala Tyr Asp Gly Thr 
        115                 120                 125             
Ile Leu Lys Ser Thr Asn Ile Ile Ala Arg Thr Asn Pro Asn Ala Gln 
    130                 135                 140                 
Arg Arg Ile Leu Leu Cys Ala His Trp Asp Ser Arg Pro Trp Ala Asp 
145                 150                 155                 160 
Asn Asp Pro Asp Ser Thr Asn His Lys Lys Pro Val Met Ala Ala Asn 
                165                 170                 175     
Asp Gly Ala Ser Gly Val Gly Val Met Ile Glu Leu Ala Arg Gln Leu 
            180                 185                 190         
Gln Ala Asp Ser Thr Leu Asn Val Gly Val Asp Phe Val Cys Phe Asp 
        195                 200                 205             
Ala Glu Asp Trp Gly Val Pro Gln Trp Glu Thr Asn Tyr Gln Glu Gln 
    210                 215                 220                 
Ser Gly Asp Ser Trp Ala Leu Gly Ser Asn Tyr Phe Ala Lys Asn Leu 
225                 230                 235                 240 
Pro Leu Thr Val Arg Pro Glu Phe Gly Ile Leu Leu Asp Met Val Gly 
                245                 250                 255     
Gly Glu Gly Ala Gln Phe Tyr Lys Glu Gly Ile Ser Leu Gln Tyr Ala 
            260                 265                 270         
Pro Asp Ile Val Asp Arg Val Trp Glu Ala Ala Lys Ser Ala Gly Phe 
        275                 280                 285             
Glu Ala Tyr Phe Pro Thr Thr Arg Gly Gly Met Val Thr Asp Asp His 
    290                 295                 300                 
Tyr Pro Leu Asn Lys Ile Ala Ala Ile Pro Thr Ile Asp Ile Ile Pro 
305                 310                 315                 320 
His Tyr Pro Asp Cys Ala Gln Ser Thr Phe Gly Pro Thr Trp His Thr 
                325                 330                 335     
Val Asn Asp Thr Met Glu His Ile Asp Arg Thr Thr Leu Gln Ala Val 
            340                 345                 350         
Gly Gln Thr Leu Ile Gln Val Leu Tyr Ser Met 
        355                 360             

<210> 3
<211> 333
<212> PRT
<213> Tannerella forsythia


<220> 
<223> TfQC

<400> 3
Met Asp Arg Met Ile Asn Lys Tyr Ala Gly Val Leu Leu Gly Ser Leu 
1               5                   10                  15      
Ile Leu Ser Cys Cys Gly Gln Lys Asn Thr Thr Lys Glu Glu Thr Thr 
            20                  25                  30          
Glu Pro Ala Asp Thr Asp Lys Arg Ile Glu Ala Pro Thr Phe Asn Ala 
        35                  40                  45              
Asp Ser Ala Tyr Ala Tyr Ile Glu Arg Gln Val Ala Phe Gly Pro Arg 
    50                  55                  60                  
Val Pro Asn Thr Glu Ala His Gln Arg Cys Ala Asp Tyr Leu Ala Gly 
65                  70                  75                  80  
Glu Leu Asp Arg His Gly Ala Lys Val Tyr Val Gln Glu Ala Val Leu 
                85                  90                  95      
Thr Ala Tyr Asn Gly Glu Lys Leu Lys Ala Gln Asn Ile Val Gly Ala 
            100                 105                 110         
Phe Gln Pro Glu Lys Ser Arg Arg Val Leu Leu Phe Ala His Trp Asp 
        115                 120                 125             
Ser Arg Pro Tyr Ala Asp His Asp Thr Asp Glu Ala Asn His Arg Lys 
    130                 135                 140                 
Pro Ile Asp Gly Ala Asp Asp Gly Gly Ser Gly Val Gly Ile Leu Leu 
145                 150                 155                 160 
Glu Ile Ala Arg Gln Ile Gln Ala Lys Ala Pro Ala Ile Gly Ile Asp 
                165                 170                 175     
Ile Val Phe Phe Asp Ala Glu Asp Tyr Gly Thr Pro Glu Phe Val Asp 
            180                 185                 190         
Glu Tyr Lys Pro Asp Thr Trp Cys Leu Gly Ser Gln Phe Trp Ala Lys 
        195                 200                 205             
Asn Pro His Val Pro Asn Tyr Lys Ala Glu Phe Gly Ile Leu Leu Asp 
    210                 215                 220                 
Met Val Gly Ser Arg Gly Ala Thr Phe Tyr Lys Glu Ser Thr Ser Val 
225                 230                 235                 240 
Gln Tyr Ala Ala Arg Tyr Val Glu Lys Val Trp Thr Ala Ala Arg Glu 
                245                 250                 255     
Leu Gly Tyr Gly Lys Tyr Phe Ile Asn Ala Gln Gly Gly Ala Ile Val 
            260                 265                 270         
Asp Asp His Gln Tyr Val Ile Gln Gly Leu Arg Thr Pro Cys Leu Asp 
        275                 280                 285             
Ile Ile Asn Tyr Asp Pro Asp Thr Gln Ser Gly Phe Gly Pro Tyr Trp 
    290                 295                 300                 
His Thr Gln Asn Asp Thr Met Glu Asn Ile Asp Arg Glu Thr Leu Lys 
305                 310                 315                 320 
Ala Val Gly Glu Thr Ile Leu Asn Val Ile Tyr Asn His 
                325                 330             

<210> 4
<211> 361
<212> PRT
<213> Homo sapiens


<220> 
<223> hQC

<400> 4
Met Ala Gly Gly Arg His Arg Arg Val Val Gly Thr Leu His Leu Leu 
1               5                   10                  15      
Leu Leu Val Ala Ala Leu Pro Trp Ala Ser Arg Gly Val Ser Pro Ser 
            20                  25                  30          
Ala Ser Ala Trp Pro Glu Glu Lys Asn Tyr His Gln Pro Ala Ile Leu 
        35                  40                  45              
Asn Ser Ser Ala Leu Arg Gln Ile Ala Glu Gly Thr Ser Ile Ser Glu 
    50                  55                  60                  
Met Trp Gln Asn Asp Leu Gln Pro Leu Leu Ile Glu Arg Tyr Pro Gly 
65                  70                  75                  80  
Ser Pro Gly Ser Tyr Ala Ala Arg Gln His Ile Met Gln Arg Ile Gln 
                85                  90                  95      
Arg Leu Gln Ala Asp Trp Val Leu Glu Ile Asp Thr Phe Leu Ser Gln 
            100                 105                 110         
Thr Pro Tyr Gly Tyr Arg Ser Phe Ser Asn Ile Ile Ser Thr Leu Asn 
        115                 120                 125             
Pro Thr Ala Lys Arg His Leu Val Leu Ala Cys His Tyr Asp Ser Lys 
    130                 135                 140                 
Tyr Phe Ser His Trp Asn Asn Arg Val Phe Val Gly Ala Thr Asp Ser 
145                 150                 155                 160 
Ala Val Pro Cys Ala Met Met Leu Glu Leu Ala Arg Ala Leu Asp Lys 
                165                 170                 175     
Lys Leu Leu Ser Leu Lys Thr Val Ser Asp Ser Lys Pro Asp Leu Ser 
            180                 185                 190         
Leu Gln Leu Ile Phe Phe Asp Gly Glu Glu Ala Phe Leu His Trp Ser 
        195                 200                 205             
Pro Gln Asp Ser Leu Tyr Gly Ser Arg His Leu Ala Ala Lys Met Ala 
    210                 215                 220                 
Ser Thr Pro His Pro Pro Gly Ala Arg Gly Thr Ser Gln Leu His Gly 
225                 230                 235                 240 
Met Asp Leu Leu Val Leu Leu Asp Leu Ile Gly Ala Pro Asn Pro Thr 
                245                 250                 255     
Phe Pro Asn Phe Phe Pro Asn Ser Ala Arg Trp Phe Glu Arg Leu Gln 
            260                 265                 270         
Ala Ile Glu His Glu Leu His Glu Leu Gly Leu Leu Lys Asp His Ser 
        275                 280                 285             
Leu Glu Gly Arg Tyr Phe Gln Asn Tyr Ser Tyr Gly Gly Val Ile Gln 
    290                 295                 300                 
Asp Asp His Ile Pro Phe Leu Arg Arg Gly Val Pro Val Leu His Leu 
305                 310                 315                 320 
Ile Pro Ser Pro Phe Pro Glu Val Trp His Thr Met Asp Asp Asn Glu 
                325                 330                 335     
Glu Asn Leu Asp Glu Ser Thr Ile Asp Asn Leu Asn Lys Ile Leu Gln 
            340                 345                 350         
Val Phe Val Leu Glu Tyr Leu His Leu 
        355                 360     

<210> 5
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> pgQC NdeI forward

<400> 5
aaacatatga acggcaataa cacaagtgaa                                     30


<210> 6
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> pgQC NheI reverse

<400> 6
tttgctagct cagtgtgaag cggcttt                                        27


<210> 7
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> piQC NdeI forward

<400> 7
tttcatatga aaggaaaatc gtctaac                                        27


<210> 8
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> piQC NheI reverse

<400> 8
atgctagctt acatgctgta aagcac                                         26


<210> 9
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> tfQC NdeI forward

<400> 9
tcacatatgg gtcagaaaaa tacgaca                                        27


<210> 10
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> tfQC NheI reverse

<400> 10
atgctagctt atttctcatt ataaatcac                                      29


<210> 11
<211> 90
<212> DNA
<213> Artificial Sequence


<220> 
<223> seq pgQC NdeI forward

<400> 11
aaacatatga aaagactgat aacaacagga gcagcctttc tactggctgc tacactctct     60

gcctgcaacg gcaataacac aagtgaaacg                                      90


<210> 12
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> seq pgQC XhoI reverse

<400> 12
tttctcgagg tgtgaagcgg ctttcac                                        27


<210> 13
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> seq pgQC RBS NotI forward

<400> 13
tggcggccgc taagaaggag a                                              21


<210> 14
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> seq pgQC XbaI reverse

<400> 14
ttttctagag tgtgaagcgg ctttcac                                        27


