                         SEQUENCE LISTING

<110>  KWS SAAT SE
 
<120>  Conferring Resistance To Geminiviruses In Plants In Alternative 
       Manner To Gene Drive, Using Crispr/Cas Systems

<130>  KWS0248PCT

<150>  US 62/436,792
<151>  2016-12-20

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  13
<212>  DNA
<213>  Tomato golden mosaic virus

<400>  1
ggtagtaagg tag                                                          13


<210>  2
<211>  16
<212>  DNA
<213>  bean yellow dwarf virus

<400>  2
tggaggcatg gaggca                                                       16


<210>  3
<211>  12
<212>  DNA
<213>  bean yellow dwarf virus

<400>  3
ttccttacct at                                                           12


<210>  4
<211>  18
<212>  DNA
<213>  bean yellow dwarf virus

<400>  4
cactatcatg ctcttctc                                                     18


<210>  5
<211>  21
<212>  DNA
<213>  bean yellow dwarf virus

<400>  5
gtccttgatt acatatcaaa g                                                 21


<210>  6
<211>  21
<212>  DNA
<213>  Tomato golden mosaic virus

<400>  6
tttcttacat atcctcagtg c                                                 21


<210>  7
<211>  21
<212>  DNA
<213>  Tomato golden mosaic virus

<400>  7
cacctccacg tgcttattca g                                                 21


<210>  8
<211>  21
<212>  DNA
<213>  Tomato golden mosaic virus

<400>  8
gtcaagacgt acatcgacaa a                                                 21


<210>  9
<211>  23
<212>  DNA
<213>  bean yellow dwarf virus

<400>  9
gcgacaaggg ggggcccacg ccg                                               23


<210>  10
<211>  26
<212>  DNA
<213>  cabbage leaf curl virus

<400>  10
atgtatccta caaagtttag gcgtgg                                            26


<210>  11
<211>  24
<212>  DNA
<213>  cabbage leaf curl virus

<400>  11
ctatgtaatt aaacgcattt ggag                                              24


<210>  12
<211>  13
<212>  DNA
<213>  Tomato golden mosaic virus

<400>  12
ggtagtaagg tag                                                          13


<210>  13
<211>  16
<212>  DNA
<213>  bean yellow dwarf virus

<400>  13
tggaggcatg gaggca                                                       16


<210>  14
<211>  12
<212>  DNA
<213>  bean yellow dwarf virus

<400>  14
ataggtaagg aa                                                           12


<210>  15
<211>  21
<212>  DNA
<213>  bean yellow dwarf virus

<400>  15
ctggagaaga gcatgatagt g                                                 21


<210>  16
<211>  21
<212>  DNA
<213>  bean yellow dwarf virus

<400>  16
ctttgatatg taatcaagga c                                                 21


<210>  17
<211>  15
<212>  DNA
<213>  bean yellow dwarf virus

<400>  17
ttcgtggcag cgaag                                                        15


<210>  18
<211>  23
<212>  DNA
<213>  bean yellow dwarf virus

<400>  18
gcgacaaggg ggggcccacg ccg                                               23


<210>  19
<211>  26
<212>  DNA
<213>  cabbage leaf curl virus

<400>  19
atgtatccta caaagtttag gcgtgg                                            26


<210>  20
<211>  20
<212>  DNA
<213>  cabbage leaf curl virus

<400>  20
ctatgtaatt aaacgcattt                                                   20


<210>  21
<211>  13
<212>  DNA
<213>  Tomato golden mosaic virus

<400>  21
ctaccttact acc                                                          13


<210>  22
<211>  16
<212>  DNA
<213>  bean yellow dwarf virus

<400>  22
tgcctccatg cctcca                                                       16


<210>  23
<211>  12
<212>  DNA
<213>  bean yellow dwarf virus

<400>  23
ttccttacct at                                                           12


<210>  24
<211>  21
<212>  DNA
<213>  bean yellow dwarf virus

<400>  24
cactatcatg ctcttctcca g                                                 21


<210>  25
<211>  21
<212>  DNA
<213>  bean yellow dwarf virus

<400>  25
gtccttgatt acatatcaaa g                                                 21


<210>  26
<211>  15
<212>  DNA
<213>  bean yellow dwarf virus

<400>  26
cttcgctgcc acgaa                                                        15


<210>  27
<211>  23
<212>  DNA
<213>  bean yellow dwarf virus

<400>  27
cggcgtgggc ccccccttgt cgc                                               23


<210>  28
<211>  26
<212>  DNA
<213>  cabbage leaf curl virus

<400>  28
ccacgcctaa actttgtagg atacat                                            26


<210>  29
<211>  20
<212>  DNA
<213>  cabbage leaf curl virus

<400>  29
aaatgcgttt aattacatag                                                   20


<210>  30
<211>  1366
<212>  DNA
<213>  cotton leaf curl virus

<400>  30
accgtgggcg agcggtgtct ttggcgttcc atgtgggtcc cacaatatcc aaaagaagaa       60

taatggactg ggtcaatgca attgggcctt aaatgaaatg ggcttggacc agtagattcg      120

agactgggcc aatagaataa aacaacaaat ggactcataa tcaaaacaaa gtgtttattc      180

atgtcaaata cattacacac tcacacacac acagtcgtac acacatcata ttcatcccct      240

atacgtatat caactaatgg ggcctcatgc atcatcatta tatcaatagc ctctaccatg      300

tcctcctggc gaaagtcccg aacatgacaa tccctataca tgatctttaa tatattatgt      360

atcccttcct ccaaattgtt gaagtcgaaa ggcggtatga tcccatcatg gccgtatggg      420

atcatgaagg tcttctttgc cagggcaggt gatcttgttg agcacaattc aatccgcaca      480

aggattgaat tgtcctcgtg gatcttcacg tcgacggtaa ataccatccc cttctcgtta      540

gtatacttga ttgtcatttg catttaatta tgaacaacac atgagatgaa tcttcttaaa      600

tagcgtccat atatttggat atatggacat aatgcatata cgtggtgcaa taattatcat      660

atgaatatga gtggagacat atatgattga tatctacagg actatcaatc atcgtcaaga      720

aaaaagggat aaaagaaacc ttattcatga aaggattagg tagggaaaaa taaaagaagg      780

aaattaagga aaagaaaacc cacaatgaaa taattaagaa aaaaaaaaga aaaaaagaac      840

acaagaaagg aaaacagaaa catgagcaca aacagaaacc tcttgagaaa atattggagc      900

gcagcgggaa aaccaagaaa aacaaaccaa ggaagacact tgagcaaaaa gggaaaacac      960

aaactaaact aggagggtcc aacatgaaat tatgaaaagt ccgtacacag taattaatca     1020

ttaattactg cgcagtaaat gtgaataaaa ttaacccgaa gggttaattt tcgagacccc     1080

gataggtaat tgagtcccca atatatcggg gactcaatcg gggtcatgag agagaaataa     1140

atcccggata ccgaaactac cctcaaagct gtgtctggaa ggcgcgtggg agtgcgctga     1200

aaaaggtgac cttctctctc caaaaactca ccggaacggc caaactggct gattccggca     1260

tctaatcacg acacgcgcgg cggtgtgtac ccctgggagg gtagaccact acgctacgca     1320

gcagccttag ctacgccgga gcttagctcg cccacgttct aatatt                    1366


<210>  31
<211>  4156
<212>  DNA
<213>  Streptococcus pyogenes

<400>  31
atggataaga aatactcaat aggcttagat atcggcacaa atagcgtcgg atgggcggtg       60

atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc      120

cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa      180

gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt      240

tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga      300

cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga      360

aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa      420

aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat      480

atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat      540

gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct      600

attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga      660

cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat      720

ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa      780

gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg      840

caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt      900

ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca      960

atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga     1020

caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca     1080

ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta     1140

gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc     1200

aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat     1260

gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt     1320

gaaaaaatct tgacttttcg aattccttat tatgttggtc cattggcgcg tggcaatagt     1380

cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa     1440

gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa     1500

aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt     1560

tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt     1620

tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc     1680

gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt     1740

tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt     1800

attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt     1860

ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct     1920

cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga     1980

cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta     2040

gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat     2100

agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta     2160

catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact     2220

gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt     2280

attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt     2340

atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct     2400

gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga     2460

gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatcac     2520

attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct     2580

gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa     2640

aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta     2700

acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa     2760

ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat     2820

actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct     2880

aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat     2940

taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa     3000

tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa     3060

atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct     3120

aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc     3180

cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt     3240

gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagta     3300

cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt     3360

gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct     3420

tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt     3480

aaagagttac tagggatcac aattatggaa agaagttcct ttgaaaaaaa tccgattgac     3540

tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa     3600

tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta     3660

caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt     3720

cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag     3780

cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt     3840

attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa     3900

ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct     3960

cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa     4020

gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt     4080

gatttgagtc agctaggagg tgacggttct cccaagaaga agaggaaagt ctcgagcggt     4140

ggagctgcag gatgag                                                     4156


<210>  32
<211>  105
<212>  DNA
<213>  bean yellow dwarf virus

<400>  32
atatgtaatc aaggacttgt ttagagtttc tagctggctg gatattaggg tgatttcctt       60

caaaatcgaa aaaagaagga tccctaatac aaggtttttt atcaa                      105


<210>  33
<211>  102
<212>  DNA
<213>  bean yellow dwarf virus

<400>  33
ggtagtgcca tcttgatgaa gctcagaagc aacaccaagg aagaaaataa gaaaaggtgt       60

gagtttctcc cagagaaact gaataaatca tctctttgag at                         102


<210>  34
<211>  4363
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct including the Cas9 coding sequence flanked by both the 
       left and right homology arms, operatively linked to the RNA 
       polymerase II promoter

<400>  34
atatgtaatc aaggacttgt ttagagtttc tagctggctg gatattaggg tgatttcctt       60

caaaatcgaa aaaagaagga tccctaatac aaggtttttt atcaaatgga taagaaatac      120

tcaataggct tagatatcgg cacaaatagc gtcggatggg cggtgatcac tgatgaatat      180

aaggttccgt ctaaaaagtt caaggttctg ggaaatacag accgccacag tatcaaaaaa      240

aatcttatag gggctctttt atttgacagt ggagagacag cggaagcgac tcgtctcaaa      300

cggacagctc gtagaaggta tacacgtcgg aagaatcgta tttgttatct acaggagatt      360

ttttcaaatg agatggcgaa agtagatgat agtttctttc atcgacttga agagtctttt      420

ttggtggaag aagacaagaa gcatgaacgt catcctattt ttggaaatat agtagatgaa      480

gttgcttatc atgagaaata tccaactatc tatcatctgc gaaaaaaatt ggtagattct      540

actgataaag cggatttgcg cttaatctat ttggccttag cgcatatgat taagtttcgt      600

ggtcattttt tgattgaggg agatttaaat cctgataata gtgatgtgga caaactattt      660

atccagttgg tacaaaccta caatcaatta tttgaagaaa accctattaa cgcaagtgga      720

gtagatgcta aagcgattct ttctgcacga ttgagtaaat caagacgatt agaaaatctc      780

attgctcagc tccccggtga gaagaaaaat ggcttatttg ggaatctcat tgctttgtca      840

ttgggtttga cccctaattt taaatcaaat tttgatttgg cagaagatgc taaattacag      900

ctttcaaaag atacttacga tgatgattta gataatttat tggcgcaaat tggagatcaa      960

tatgctgatt tgtttttggc agctaagaat ttatcagatg ctattttact ttcagatatc     1020

ctaagagtaa atactgaaat aactaaggct cccctatcag cttcaatgat taaacgctac     1080

gatgaacatc atcaagactt gactctttta aaagctttag ttcgacaaca acttccagaa     1140

aagtataaag aaatcttttt tgatcaatca aaaaacggat atgcaggtta tattgatggg     1200

ggagctagcc aagaagaatt ttataaattt atcaaaccaa ttttagaaaa aatggatggt     1260

actgaggaat tattggtgaa actaaatcgt gaagatttgc tgcgcaagca acggaccttt     1320

gacaacggct ctattcccca tcaaattcac ttgggtgagc tgcatgctat tttgagaaga     1380

caagaagact tttatccatt tttaaaagac aatcgtgaga agattgaaaa aatcttgact     1440

tttcgaattc cttattatgt tggtccattg gcgcgtggca atagtcgttt tgcatggatg     1500

actcggaagt ctgaagaaac aattacccca tggaattttg aagaagttgt cgataaaggt     1560

gcttcagctc aatcatttat tgaacgcatg acaaactttg ataaaaatct tccaaatgaa     1620

aaagtactac caaaacatag tttgctttat gagtatttta cggtttataa cgaattgaca     1680

aaggtcaaat atgttactga aggaatgcga aaaccagcat ttctttcagg tgaacagaag     1740

aaagccattg ttgatttact cttcaaaaca aatcgaaaag taaccgttaa gcaattaaaa     1800

gaagattatt tcaaaaaaat agaatgtttt gatagtgttg aaatttcagg agttgaagat     1860

agatttaatg cttcattagg tacctaccat gatttgctaa aaattattaa agataaagat     1920

tttttggata atgaagaaaa tgaagatatc ttagaggata ttgttttaac attgacctta     1980

tttgaagata gggagatgat tgaggaaaga cttaaaacat atgctcacct ctttgatgat     2040

aaggtgatga aacagcttaa acgtcgccgt tatactggtt ggggacgttt gtctcgaaaa     2100

ttgattaatg gtattaggga taagcaatct ggcaaaacaa tattagattt tttgaaatca     2160

gatggttttg ccaatcgcaa ttttatgcag ctgatccatg atgatagttt gacatttaaa     2220

gaagacattc aaaaagcaca agtgtctgga caaggcgata gtttacatga acatattgca     2280

aatttagctg gtagccctgc tattaaaaaa ggtattttac agactgtaaa agttgttgat     2340

gaattggtca aagtaatggg gcggcataag ccagaaaata tcgttattga aatggcacgt     2400

gaaaatcaga caactcaaaa gggccagaaa aattcgcgag agcgtatgaa acgaatcgaa     2460

gaaggtatca aagaattagg aagtcagatt cttaaagagc atcctgttga aaatactcaa     2520

ttgcaaaatg aaaagctcta tctctattat ctccaaaatg gaagagacat gtatgtggac     2580

caagaattag atattaatcg tttaagtgat tatgatgtcg atcacattgt tccacaaagt     2640

ttccttaaag acgattcaat agacaataag gtcttaacgc gttctgataa aaatcgtggt     2700

aaatcggata acgttccaag tgaagaagta gtcaaaaaga tgaaaaacta ttggagacaa     2760

cttctaaacg ccaagttaat cactcaacgt aagtttgata atttaacgaa agctgaacgt     2820

ggaggtttga gtgaacttga taaagctggt tttatcaaac gccaattggt tgaaactcgc     2880

caaatcacta agcatgtggc acaaattttg gatagtcgca tgaatactaa atacgatgaa     2940

aatgataaac ttattcgaga ggttaaagtg attaccttaa aatctaaatt agtttctgac     3000

ttccgaaaag atttccaatt ctataaagta cgtgagatta acaattacca tcatgcccat     3060

gatgcgtatc taaatgccgt cgttggaact gctttgatta agaaatatcc aaaacttgaa     3120

tcggagtttg tctatggtga ttataaagtt tatgatgttc gtaaaatgat tgctaagtct     3180

gagcaagaaa taggcaaagc aaccgcaaaa tatttctttt actctaatat catgaacttc     3240

ttcaaaacag aaattacact tgcaaatgga gagattcgca aacgccctct aatcgaaact     3300

aatggggaaa ctggagaaat tgtctgggat aaagggcgag attttgccac agtgcgcaaa     3360

gtattgtcca tgccccaagt caatattgtc aagaaaacag aagtacagac aggcggattc     3420

tccaaggagt caattttacc aaaaagaaat tcggacaagc ttattgctcg taaaaaagac     3480

tgggatccaa aaaaatatgg tggttttgat agtccaacgg tagcttattc agtcctagtg     3540

gttgctaagg tggaaaaagg gaaatcgaag aagttaaaat ccgttaaaga gttactaggg     3600

atcacaatta tggaaagaag ttcctttgaa aaaaatccga ttgacttttt agaagctaaa     3660

ggatataagg aagttaaaaa agacttaatc attaaactac ctaaatatag tctttttgag     3720

ttagaaaacg gtcgtaaacg gatgctggct agtgccggag aattacaaaa aggaaatgag     3780

ctggctctgc caagcaaata tgtgaatttt ttatatttag ctagtcatta tgaaaagttg     3840

aagggtagtc cagaagataa cgaacaaaaa caattgtttg tggagcagca taagcattat     3900

ttagatgaga ttattgagca aatcagtgaa ttttctaagc gtgttatttt agcagatgcc     3960

aatttagata aagttcttag tgcatataac aaacatagag acaaaccaat acgtgaacaa     4020

gcagaaaata ttattcattt atttacgttg acgaatcttg gagctcccgc tgcttttaaa     4080

tattttgata caacaattga tcgtaaacga tatacgtcta caaaagaagt tttagatgcc     4140

actcttatcc atcaatccat cactggtctt tatgaaacac gcattgattt gagtcagcta     4200

ggaggtgacg gttctcccaa gaagaagagg aaagtctcga gcggtggagc tgcaggatga     4260

gggtagtgcc atcttgatga agctcagaag caacaccaag gaagaaaata agaaaaggtg     4320

tgagtttctc ccagagaaac tgaataaatc atctctttga gat                       4363


<210>  35
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA comprising SEQ ID NO: 15 as target sequence

<400>  35
aatttctact cttgtagatc tggagaagag catgatagtg                             40


<210>  36
<211>  87
<212>  DNA
<213>  Streptococcus pyogenes

<400>  36
gttggaacca ttcaaaacag catagcaagt taaaataagg ctagtccgtt atcaacttga       60

aaaagtggca ccgagtcggt gcttttt                                           87


