﻿                         序列表

<110>  广东溢多利生物科技股份有限公司

<120>  活性提高的α-淀粉酶AmyL突变体及其编码基因和应用

<160>  3

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  486
<212>  PRT
<213>  盐地咸海鲜芽胞杆菌

<400>  1
Ser Thr Pro Asn Asn Gly Thr Leu Met Gln Tyr Phe Glu Trp Tyr Leu 
1               5                   10                  15      
Pro Asn Asp Gly Ala His Trp Gln Arg Leu His Asn Asp Ala Gly Asn 
            20                  25                  30          
Leu Ala Asn Lys Gly Ile Ser Ala Val Trp Ile Pro Pro Ala Tyr Lys 
        35                  40                  45              
Gly Thr Ser Gln Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr Asp 
    50                  55                  60                  
Leu Gly Glu Phe Asn Gln Lys Gly Thr Ile Arg Thr Lys Tyr Gly Thr 
65                  70                  75                  80  
Lys Ala Gln Leu Lys Ser Ala Ile Ser Ala Leu Gln Ser Gln Asn Ile 
                85                  90                  95      
Asn Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp Phe 
            100                 105                 110         
Thr Gln Pro Val Thr Val Val Gln Val Asp Pro Asn Asn Arg Asn Asn 
        115                 120                 125             
Glu Ile Ser Gly Asp Tyr Gln Ile Asp Ala Trp Thr Gly Phe Asn Phe 
    130                 135                 140                 
Pro Gly Arg Gly Asn Thr Tyr Ser Asn Phe Lys Trp Ser Trp Tyr His 
145                 150                 155                 160 
Phe Asp Gly Thr Asp Trp Asp Glu Arg Arg Asp Gln Ser Gly Val Tyr 
                165                 170                 175     
Lys Phe Arg Gly Thr Gly Lys Ala Trp Asp Trp Glu Val Ser Asn Glu 
            180                 185                 190         
Arg Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Leu Asp Phe Ser His 
        195                 200                 205             
Pro Asp Val Ala Asn Glu Met Lys Asn Trp Gly Thr Trp Tyr Ala Asn 
    210                 215                 220                 
Glu Leu Asn Leu Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile Lys 
225                 230                 235                 240 
His Asp Tyr Leu Gly Glu Trp Val Ser His Val Arg Ser Gln Thr Gly 
                245                 250                 255     
Lys Asn Leu Phe Thr Val Ala Glu Tyr Trp Gln Asn Asp Ile Asn Ala 
            260                 265                 270         
Leu Asn Asn Tyr Leu Ala Lys Thr Asn Tyr Asn His Ser Ile Phe Asp 
        275                 280                 285             
Ala Pro Leu His Tyr Asn Phe His Tyr Ala Ser Asn Ser Asn Gly Asn 
    290                 295                 300                 
Tyr Asp Met Arg Asn Ile Leu Asn Gly Thr Val Val Lys Gln His Pro 
305                 310                 315                 320 
Ser Leu Ala Val Thr Leu Val Glu Asn His Asp Ser Gln Pro Gly Gln 
                325                 330                 335     
Ala Leu Glu Ser Val Val Gly Thr Trp Phe Lys Pro Leu Ala Tyr Ala 
            340                 345                 350         
Phe Ile Leu Thr Arg Gln Gln Gly Tyr Pro Ser Val Phe Tyr Gly Asp 
        355                 360                 365             
Tyr Tyr Gly Thr Asn Gly Asp Ser Ser Tyr Glu Ile Pro Ser Leu Lys 
    370                 375                 380                 
Asn Lys Ile Asp Pro Leu Leu Thr Ala Arg Lys Asn Phe Ala Tyr Gly 
385                 390                 395                 400 
Val Gln His Asp Tyr Leu Asp His Trp Asp Ile Ile Gly Trp Thr Arg 
                405                 410                 415     
Glu Gly Glu Gly Thr Arg Gln Lys Ser Gly Leu Ala Thr Leu Ile Thr 
            420                 425                 430         
Asp Gly Pro Gly Gly Ser Lys Trp Met Tyr Val Gly Arg Gln Asn Ala 
        435                 440                 445             
Gly Glu Val Trp Tyr Asp Met Thr Gly Asn Arg Ser Thr Thr Val Thr 
    450                 455                 460                 
Ile Asn Asn Asp Gly Trp Gly Gln Phe Tyr Val Asn Gly Gly Ser Val 
465                 470                 475                 480 
Ser Val Tyr Val Gln Lys 
                485     

<210>  2
<211>  486
<212>  PRT
<213>  人工序列

<400>  2
Ser Thr Pro Asn Asn Gly Thr Leu Met Gln Tyr Phe Glu Trp Tyr Leu 
1               5                   10                  15      
Pro Asp Asp Gly Ala His Trp Gln Arg Leu His Asn Asp Ala Gly Asn 
            20                  25                  30          
Leu Ala Asn Lys Gly Ile Asn Ala Val Trp Ile Pro Pro Ala Tyr Lys 
        35                  40                  45              
Gly Thr Ser Gln Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr Asp 
    50                  55                  60                  
Leu Gly Glu Phe Asn Gln Lys Gly Thr Ile Arg Thr Lys Tyr Gly Thr 
65                  70                  75                  80  
Lys Ala Gln Leu Lys Ser Ala Ile Ser Ala Leu Gln Ser Gln Asn Ile 
                85                  90                  95      
Asn Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp Phe 
            100                 105                 110         
Thr Gln Pro Val Thr Val Val Gln Val Asp Pro Asn Asn Arg Asn Asn 
        115                 120                 125             
Glu Ile Ser Gly Asp Tyr Gln Ile Asp Ala Trp Thr Gly Phe Asn Phe 
    130                 135                 140                 
Pro Gly Arg Gly Asn Thr Tyr Ser Asn Phe Lys Trp Ser Trp Asp His 
145                 150                 155                 160 
Phe Asp Gly Thr Asp Trp Asp Glu Arg Arg Asp Gln Ser Gly Val Tyr 
                165                 170                 175     
Lys Phe Arg Gly Thr Gly Lys Ala Trp Asp Trp Glu Val Ser Asn Glu 
            180                 185                 190         
Arg Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Leu Asp Phe Ser His 
        195                 200                 205             
Pro Asp Val Ala Asn Glu Met Lys Asn Trp Gly Lys Trp Tyr Ala Asn 
    210                 215                 220                 
Glu Leu Asn Leu Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile Lys 
225                 230                 235                 240 
His Asp Tyr Leu Gly Glu Trp Val Ser His Val Arg Ser Gln Thr Gly 
                245                 250                 255     
Lys Asn Leu Phe Thr Val Ala Glu Tyr Trp Gln Asn Asp Ile Asn Ala 
            260                 265                 270         
Leu Asn Asn Tyr Leu Ala Lys Thr Asp Tyr Asn His Ser Ile Phe Asp 
        275                 280                 285             
Ala Pro Leu His Tyr Asn Phe His Tyr Ala Ser Asn Ser Asn Gly Asn 
    290                 295                 300                 
Tyr Asp Met Arg Asn Ile Leu Asn Gly Thr Val Val Lys Gln His Pro 
305                 310                 315                 320 
Ser Leu Ala Val Thr Leu Val Glu Asn His Asp Ser Gln Pro Gly Gln 
                325                 330                 335     
Ala Leu Glu Ser Val Val Gly Thr Trp Phe Lys Pro Leu Ala Tyr Ala 
            340                 345                 350         
Phe Ile Leu Thr Arg Gln Gln Gly Tyr Pro Cys Val Phe Tyr Gly Asp 
        355                 360                 365             
Tyr Tyr Gly Thr Asn Gly Asp Ser Ser Tyr Glu Ile Pro Ser Leu Lys 
    370                 375                 380                 
Asn Lys Ile Asp Pro Leu Leu Thr Ala Arg Lys Asn Phe Ala Tyr Gly 
385                 390                 395                 400 
Val Gln His Asp Tyr Leu Asp His Trp Asp Ile Ile Gly Trp Thr Arg 
                405                 410                 415     
Glu Gly Glu Gly Thr Arg Gln Lys Ser Gly Leu Ala Thr Leu Ile Thr 
            420                 425                 430         
Asp Gly Pro Gly Gly Ser Lys Trp Met Tyr Val Gly Arg Gln Asn Ala 
        435                 440                 445             
Gly Glu Val Trp Tyr Asp Met Thr Gly Asn Arg Ser Thr Thr Val Thr 
    450                 455                 460                 
Ile Asn Asn Asp Gly Trp Gly Gln Phe Lys Val Asn Gly Gly Ser Val 
465                 470                 475                 480 
Ser Val Tyr Val Gln Lys 
                485     

<210>  3
<211>  1548
<212>  DNA
<213>  盐地咸海鲜芽胞杆菌

<400>  3
atgagacagg ttagaattgc ttttgtttta tgtttatcga ttatgctgtt tctgtctggt        60
tttggtactg tagacagagc aggagcttca acaccgaaca atggaacgtt aatgcagtat       120
ttcgaatggt atcttcctaa tgatggagct cattggcaac gcttacataa tgatgctgga       180
aatctagcaa ataaggggat ttcagctgta tggattccac cagcatacaa aggcacttca       240
caaaatgatg taggttatgg tgcttacgac ttatatgatc ttggtgagtt taatcaaaaa       300
ggaacaattc ggactaaata tggaacgaaa gcacaactaa agtcagccat ttctgccttg       360
caaagtcaaa atataaatgt atacggcgat gttgtaatga accataaagg tggtgctgat       420
tttactcagc ctgtaactgt cgtacaagtt gatccaaata accgtaataa tgaaatttct       480
ggagattatc agattgatgc ttggactggt tttaattttc ctggtagagg taatacatac       540
tctaacttca aatggtcttg gtatcatttc gatgggacag actgggatga aagaagagat       600
cagagtggag tatataaatt cagaggtact ggtaaggcat gggattggga agtgtctaac       660
gagcgaggta attatgatta cctgatgtat gctgatttag actttagcca tcctgatgtt       720
gctaatgaaa tgaaaaattg gggtacttgg tatgccaatg aactgaatct tgatggattt       780
cgtttagatg ctgttaaaca tattaagcat gattacctag gtgagtgggt tagccacgtc       840
aggtctcaga ctgggaaaaa tctgttcact gttgctgaat attggcaaaa tgatataaat       900
gcattaaata actacttggc aaaaacaaat tataaccatt cgatatttga tgcaccactt       960
cattataatt ttcattatgc atctaacagt aatggtaact atgatatgag aaacatattg      1020
aatggaacgg tcgtaaaaca gcatccatca cttgctgtaa cacttgtgga aaaccacgat      1080
tcacaaccag gtcaggctct tgagtcggtt gtaggtacat ggttcaagcc attagcgtat      1140
gcatttattt taacgcgtca acaaggttat ccttccgttt tctatggtga ctattacgga      1200
acgaatgggg acagcagcta tgaaattcca tcactaaaaa ataaaattga ccccctcctt      1260
actgcacgaa aaaactttgc atatggtgtc caacatgatt atctggatca ctgggatatt      1320
atcggctgga caagagaggg tgaaggaaca cgacagaagt ctggacttgc taccctaatc      1380
actgacggtc cgggtgggtc taagtggatg tatgtaggaa gacaaaatgc tggcgaggtc      1440
tggtatgata tgacgggtaa ccgttccact actgtcacaa taaacaatga cggctggggc      1500
cagttttatg taaatggcgg atcagtttca gtttatgtac aaaaataa                   1548

