﻿               SEQUENCE LISTING

<110> Deutsches Primatenzentrum GmbH (DPZ) - Leibniz-Institut für Primatenforschung

<120> Retargeting of Viruses or VLPs

<130> Z2186 PCT

<150> EP 17 15 4380.4
<151> 2017-02-02

<160> 32

<170> BiSSAP 1.3.6

<210> 1
<211> 1065
<212> DNA
<213> JC polyomavirus


<220> 
<223> VP1 capsid protein coding sequence

<400> 1
atggccccaa caaaaagaaa aggagaaagg aaggaccccg tgcaagttcc aaaacttctt      60

ataagaggag gagtagaagt tctagaagtt aaaactgggg ttgactcaat tacagaggta     120

gaatgctttt taactccaga aatgggtgac ccagatgagc atcttagggg ttttagtaag     180

tcaatatcta tatcagatac atttgaaagt gactccccaa atagggacat gcttccttgt     240

tacagtgtgg ccagaattcc actacccaat ctaaatgagg atctaacctg tggaaatata     300

ctcatgtggg aggctgtgac cttaaaaact gaggttatag gggtgacaag tttgatgaat     360

gtgcactcta atgggcaagc aactcatgac aatggtgcag ggaagccagt gcagggcacc     420

agctttcatt ttttttctgt tgggggggag gctttagaat tacagggggt gctttttaat     480

tacagaacaa agtacccaga tggaacaatt tttccaaaga atgccacagt gcaatctcaa     540

gtcatgaaca cagagcacaa ggcgtaccta gataagaaca aagcatatcc tgttgaatgt     600

tgggttcctg atcccaccag aaatgaaaac acaagatatt ttgggacact aacaggagga     660

gaaaatgttc ctccagttct tcatataaca aacactgcca caacagtgtt gcttgatgaa     720

tttggtgttg ggccactttg caaaggtgac aacttatact tgtcagctgt tgatgtctgt     780

ggcatgttta caaacaggtc tggttcccag cagtggagag gactctccag atattttaag     840

gtgcagctaa ggaaaaggag ggttaaaaac ccctacccaa tttctttcct tcttactgat     900

ttaattaaca gaaggactcc tagagttgat gggcagccta tgtatggcat ggatgctcaa     960

gtagaggagg ttagagtttt tgagggaaca gaggagcttc caggggaccc agacatgatg    1020

agatacgttg acaaatatgg acagttgcag acaaaaatgc tgtaa                    1065


<210> 2
<211> 354
<212> PRT
<213> JC polyomavirus


<220> 
<223> Major capsid protein VP1

<400> 2
Met Ala Pro Thr Lys Arg Lys Gly Glu Arg Lys Asp Pro Val Gln Val 
1               5                   10                  15      
Pro Lys Leu Leu Ile Arg Gly Gly Val Glu Val Leu Glu Val Lys Thr 
            20                  25                  30          
Gly Val Asp Ser Ile Thr Glu Val Glu Cys Phe Leu Thr Pro Glu Met 
        35                  40                  45              
Gly Asp Pro Asp Glu His Leu Arg Gly Phe Ser Lys Ser Ile Ser Ile 
    50                  55                  60                  
Ser Asp Thr Phe Glu Ser Asp Ser Pro Asn Arg Asp Met Leu Pro Cys 
65                  70                  75                  80  
Tyr Ser Val Ala Arg Ile Pro Leu Pro Asn Leu Asn Glu Asp Leu Thr 
                85                  90                  95      
Cys Gly Asn Ile Leu Met Trp Glu Ala Val Thr Leu Lys Thr Glu Val 
            100                 105                 110         
Ile Gly Val Thr Ser Leu Met Asn Val His Ser Asn Gly Gln Ala Thr 
        115                 120                 125             
His Asp Asn Gly Ala Gly Lys Pro Val Gln Gly Thr Ser Phe His Phe 
    130                 135                 140                 
Phe Ser Val Gly Gly Glu Ala Leu Glu Leu Gln Gly Val Leu Phe Asn 
145                 150                 155                 160 
Tyr Arg Thr Lys Tyr Pro Asp Gly Thr Ile Phe Pro Lys Asn Ala Thr 
                165                 170                 175     
Val Gln Ser Gln Val Met Asn Thr Glu His Lys Ala Tyr Leu Asp Lys 
            180                 185                 190         
Asn Lys Ala Tyr Pro Val Glu Cys Trp Val Pro Asp Pro Thr Arg Asn 
        195                 200                 205             
Glu Asn Thr Arg Tyr Phe Gly Thr Leu Thr Gly Gly Glu Asn Val Pro 
    210                 215                 220                 
Pro Val Leu His Ile Thr Asn Thr Ala Thr Thr Val Leu Leu Asp Glu 
225                 230                 235                 240 
Phe Gly Val Gly Pro Leu Cys Lys Gly Asp Asn Leu Tyr Leu Ser Ala 
                245                 250                 255     
Val Asp Val Cys Gly Met Phe Thr Asn Arg Ser Gly Ser Gln Gln Trp 
            260                 265                 270         
Arg Gly Leu Ser Arg Tyr Phe Lys Val Gln Leu Arg Lys Arg Arg Val 
        275                 280                 285             
Lys Asn Pro Tyr Pro Ile Ser Phe Leu Leu Thr Asp Leu Ile Asn Arg 
    290                 295                 300                 
Arg Thr Pro Arg Val Asp Gly Gln Pro Met Tyr Gly Met Asp Ala Gln 
305                 310                 315                 320 
Val Glu Glu Val Arg Val Phe Glu Gly Thr Glu Glu Leu Pro Gly Asp 
                325                 330                 335     
Pro Asp Met Met Arg Tyr Val Asp Lys Tyr Gly Gln Leu Gln Thr Lys 
            340                 345                 350         
Met Leu 
        

<210> 3
<211> 1089
<212> DNA
<213> Artificial Sequence


<220> 
<223> Polyoma VP1-Mut2

<400> 3
atggccccaa caaaaagaaa aggagaatgt ccaggggcag ctcccaaaaa accaaaggac      60

cccgtgcaag ttccaaaact tcttataaga ggaggagtag aagttctaga agttaaaact     120

ggggttgact caattacaga ggtagaatgc tttttaactc cagaaatggg tgacccagat     180

gagcatctta ggggttttag taagtcaatt tctatatcag atacatttga aagtgactcc     240

ccaaataagg acatgcttcc ttgttacagt gtggccagaa ttccactacc caatctaaat     300

gaggatctaa cctgtggaaa tatactaatg tgggaggctg tgaccttaaa aactgaggtt     360

ttaggggtga caactttgat gaatgtgcac tctaatggtc aagcaactca tgacaatggt     420

gcaggaaagc cagtgcaggg caccagcttt catttttttt ctgttggggg ggaggcttta     480

gaattacagg gggtggtttt taactacaga acaaagtacc cagatggaac aatttttcca     540

aagaatgcaa cagtgcaatc tcaagtaatg aacacagagc acaaggcgta cctagataag     600

aacaaagcat atcctgttga atgttgggtt cctgatccca ccagaaatga aaacacaaga     660

tattttggga cactaacagg aggagaaaat gttcctccag ttcttcatat aacaaacact     720

gccacaacag tgctgcttga tgaatttggt gttgggccac tttgcaaagg tgacaacttg     780

tatttgtcag ctgttgatgt ttgtggaatg tttactaaca gatctggtac ccagcagtgg     840

agaggactgt ccagatattt taaggttcag ctgagaaaaa ggagggttaa aaacccctac     900

ccaatttctt tccttcttac tgatttgatt aacagaagga cccctagagt tgatgggcag     960

cctatgtatg gtatggatgc tcaggtagag gaggttagag tttttgaggg gacagaggaa    1020

cttccagggg acccagacat gatgagatat gttgacagat atggacagtt gcaaacaaag    1080

atgctgtaa                                                            1089


<210> 4
<211> 362
<212> PRT
<213> Artificial Sequence


<220> 
<223> VP1-Mut2

<400> 4
Met Ala Pro Thr Lys Arg Lys Gly Glu Cys Pro Gly Ala Ala Pro Lys 
1               5                   10                  15      
Lys Pro Lys Asp Pro Val Gln Val Pro Lys Leu Leu Ile Arg Gly Gly 
            20                  25                  30          
Val Glu Val Leu Glu Val Lys Thr Gly Val Asp Ser Ile Thr Glu Val 
        35                  40                  45              
Glu Cys Phe Leu Thr Pro Glu Met Gly Asp Pro Asp Glu His Leu Arg 
    50                  55                  60                  
Gly Phe Ser Lys Ser Ile Ser Ile Ser Asp Thr Phe Glu Ser Asp Ser 
65                  70                  75                  80  
Pro Asn Lys Asp Met Leu Pro Cys Tyr Ser Val Ala Arg Ile Pro Leu 
                85                  90                  95      
Pro Asn Leu Asn Glu Asp Leu Thr Cys Gly Asn Ile Leu Met Trp Glu 
            100                 105                 110         
Ala Val Thr Leu Lys Thr Glu Val Leu Gly Val Thr Thr Leu Met Asn 
        115                 120                 125             
Val His Ser Asn Gly Gln Ala Thr His Asp Asn Gly Ala Gly Lys Pro 
    130                 135                 140                 
Val Gln Gly Thr Ser Phe His Phe Phe Ser Val Gly Gly Glu Ala Leu 
145                 150                 155                 160 
Glu Leu Gln Gly Val Val Phe Asn Tyr Arg Thr Lys Tyr Pro Asp Gly 
                165                 170                 175     
Thr Ile Phe Pro Lys Asn Ala Thr Val Gln Ser Gln Val Met Asn Thr 
            180                 185                 190         
Glu His Lys Ala Tyr Leu Asp Lys Asn Lys Ala Tyr Pro Val Glu Cys 
        195                 200                 205             
Trp Val Pro Asp Pro Thr Arg Asn Glu Asn Thr Arg Tyr Phe Gly Thr 
    210                 215                 220                 
Leu Thr Gly Gly Glu Asn Val Pro Pro Val Leu His Ile Thr Asn Thr 
225                 230                 235                 240 
Ala Thr Thr Val Leu Leu Asp Glu Phe Gly Val Gly Pro Leu Cys Lys 
                245                 250                 255     
Gly Asp Asn Leu Tyr Leu Ser Ala Val Asp Val Cys Gly Met Phe Thr 
            260                 265                 270         
Asn Arg Ser Gly Thr Gln Gln Trp Arg Gly Leu Ser Arg Tyr Phe Lys 
        275                 280                 285             
Val Gln Leu Arg Lys Arg Arg Val Lys Asn Pro Tyr Pro Ile Ser Phe 
    290                 295                 300                 
Leu Leu Thr Asp Leu Ile Asn Arg Arg Thr Pro Arg Val Asp Gly Gln 
305                 310                 315                 320 
Pro Met Tyr Gly Met Asp Ala Gln Val Glu Glu Val Arg Val Phe Glu 
                325                 330                 335     
Gly Thr Glu Glu Leu Pro Gly Asp Pro Asp Met Met Arg Tyr Val Asp 
            340                 345                 350         
Arg Tyr Gly Gln Leu Gln Thr Lys Met Leu 
        355                 360         

<210> 5
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Nuclear localization signal

<400> 5
Cys Pro Gly Ala Ala Pro 
1               5       

<210> 6
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> substance-P

<400> 6
Arg Pro Lys Pro Gln Gln Phe Phe Gly Leu Met 
1               5                   10      

<210> 7
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptide

<400> 7
Gln Glu Ile Asn Ser Ser Tyr 
1               5           

<210> 8
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptide

<400> 8
Ser His Pro Arg Leu Ser Ala 
1               5           

<210> 9
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptide

<400> 9
Ser Met Pro Asn Pro Met Val 
1               5           

<210> 10
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptide

<400> 10
Gly Leu Gln Gln Val Leu Leu 
1               5           

<210> 11
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptide

<400> 11
His Glu Leu Ser Val Leu Leu 
1               5           

<210> 12
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptide

<400> 12
Tyr Ala Pro Gln Arg Leu Pro 
1               5           

<210> 13
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptide

<400> 13
Thr Pro Arg Thr Leu Pro Thr 
1               5           

<210> 14
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptide

<400> 14
Ala Pro Val His Ser Ser Ile 
1               5           

<210> 15
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptide

<400> 15
Ala Pro Pro His Ala Leu Ser 
1               5           

<210> 16
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptide

<400> 16
Thr Phe Ser Asn Arg Phe Ile 
1               5           

<210> 17
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptide

<400> 17
Val Val Pro Thr Pro Pro Tyr 
1               5           

<210> 18
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptide

<400> 18
Glu Leu Ala Pro Asp Ser Pro 
1               5           

<210> 19
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward primer for CAG-GFP expression construct

<400> 19
gatcgtacca ttgacgtcaa taatg                                          25


<210> 20
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer for CAG-GFP expression construct

<400> 20
tctccccctg aacctgaaac                                                20


<210> 21
<211> 17
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward primer for woodchuck hepatitis virus posttranslational
      regulatory element (WPRE)

<400> 21
tcgataccgt cgacccg                                                   17


<210> 22
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer for woodchuck hepatitis virus posttranslational
      regulatory element (WPRE)

<400> 22
ttatcgataa gcttgatatc gaattc                                         26


<210> 23
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> b2m forward primer

<400> 23
tgtgctcgcg ctactctctc t                                              21


<210> 24
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> b2m reverse primer

<400> 24
cggatggatg aaacccagac a                                              21


<210> 25
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Ht2a forward primer

<400> 25
aactccagaa ctaaggcatt t                                              21


<210> 26
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Ht2a reverse primer

<400> 26
cttaaagacc ttcgaatcgt c                                              21


<210> 27
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Ht2b forward primer

<400> 27
cacgggctac agcattcatc a                                              21


<210> 28
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Ht2b reverse primer

<400> 28
ccaaaacgtt cctttgtcag c                                              21


<210> 29
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Ht2c forward primer

<400> 29
ccgagtccgt ttctcgtcta g                                              21


<210> 30
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Ht2c reverse primer

<400> 30
gatggcgtca gttggcctat g                                              21


<210> 31
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Her2/neu forward primer

<400> 31
cctctgacgt ccatcgtctc                                                20


<210> 32
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Her2/neu reverse primer

<400> 32
cggatcttct gctgccgtcg                                                20


