                         SEQUENCE LISTING

<110>  Agency for Science, Technology and Research
 
<120>  ALPHAVIRUS NSP  MUTANTS AS VACCINES

<130>  9869sg4137

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  2474
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide sequence of wild-type CHIKV LR2006 OPY1 
       non-structural polyprotein.

<400>  1

Met Asp Pro Val Tyr Val Asp Ile Asp Ala Asp Ser Ala Phe Leu Lys 
1               5                   10                  15      


Ala Leu Gln Arg Ala Tyr Pro Met Phe Glu Val Glu Pro Arg Gln Val 
            20                  25                  30          


Thr Pro Asn Asp His Ala Asn Ala Arg Ala Phe Ser His Leu Ala Ile 
        35                  40                  45              


Lys Leu Ile Glu Gln Glu Ile Asp Pro Asp Ser Thr Ile Leu Asp Ile 
    50                  55                  60                  


Gly Ser Ala Pro Ala Arg Arg Met Met Ser Asp Arg Lys Tyr His Cys 
65                  70                  75                  80  


Val Cys Pro Met Arg Ser Ala Glu Asp Pro Glu Arg Leu Ala Asn Tyr 
                85                  90                  95      


Ala Arg Lys Leu Ala Ser Ala Ala Gly Lys Val Leu Asp Arg Asn Ile 
            100                 105                 110         


Ser Gly Lys Ile Gly Asp Leu Gln Ala Val Met Ala Val Pro Asp Thr 
        115                 120                 125             


Glu Thr Pro Thr Phe Cys Leu His Thr Asp Val Ser Cys Arg Gln Arg 
    130                 135                 140                 


Ala Asp Val Ala Ile Tyr Gln Asp Val Tyr Ala Val His Ala Pro Thr 
145                 150                 155                 160 


Ser Leu Tyr His Gln Ala Ile Lys Gly Val Arg Val Ala Tyr Trp Val 
                165                 170                 175     


Gly Phe Asp Thr Thr Pro Phe Met Tyr Asn Ala Met Ala Gly Ala Tyr 
            180                 185                 190         


Pro Ser Tyr Ser Thr Asn Trp Ala Asp Glu Gln Val Leu Lys Ala Lys 
        195                 200                 205             


Asn Ile Gly Leu Cys Ser Thr Asp Leu Thr Glu Gly Arg Arg Gly Lys 
    210                 215                 220                 


Leu Ser Ile Met Arg Gly Lys Lys Leu Lys Pro Cys Asp Arg Val Leu 
225                 230                 235                 240 


Phe Ser Val Gly Ser Thr Leu Tyr Pro Glu Ser Arg Lys Leu Leu Lys 
                245                 250                 255     


Ser Trp His Leu Pro Ser Val Phe His Leu Lys Gly Lys Leu Ser Phe 
            260                 265                 270         


Thr Cys Arg Cys Asp Thr Val Val Ser Cys Glu Gly Tyr Val Val Lys 
        275                 280                 285             


Arg Ile Thr Met Ser Pro Gly Leu Tyr Gly Lys Thr Thr Gly Tyr Ala 
    290                 295                 300                 


Val Thr His His Ala Asp Gly Phe Leu Met Cys Lys Thr Thr Asp Thr 
305                 310                 315                 320 


Val Asp Gly Glu Arg Met Ser Phe Ser Val Cys Thr Tyr Val Pro Ala 
                325                 330                 335     


Thr Ile Cys Asp Gln Met Thr Gly Ile Leu Ala Thr Glu Val Thr Pro 
            340                 345                 350         


Glu Asp Ala Gln Lys Leu Leu Val Gly Leu Asn Gln Arg Ile Val Val 
        355                 360                 365             


Asn Gly Arg Thr Gln Arg Asn Thr Asn Thr Met Lys Asn Tyr Leu Leu 
    370                 375                 380                 


Pro Val Val Ala Gln Ala Phe Ser Lys Trp Ala Lys Glu Cys Arg Lys 
385                 390                 395                 400 


Asp Met Glu Asp Glu Lys Leu Leu Gly Val Arg Glu Arg Thr Leu Thr 
                405                 410                 415     


Cys Cys Cys Leu Trp Ala Phe Lys Lys Gln Lys Thr His Thr Val Tyr 
            420                 425                 430         


Lys Arg Pro Asp Thr Gln Ser Ile Gln Lys Val Gln Ala Glu Phe Asp 
        435                 440                 445             


Ser Phe Val Val Pro Ser Leu Trp Ser Ser Gly Leu Ser Ile Pro Leu 
    450                 455                 460                 


Arg Thr Arg Ile Lys Trp Leu Leu Ser Lys Val Pro Lys Thr Asp Leu 
465                 470                 475                 480 


Ile Pro Tyr Ser Gly Asp Ala Arg Glu Ala Arg Asp Ala Glu Lys Glu 
                485                 490                 495     


Ala Glu Glu Glu Arg Glu Ala Glu Leu Thr Arg Glu Ala Leu Pro Pro 
            500                 505                 510         


Leu Gln Ala Ala Gln Glu Asp Val Gln Val Glu Ile Asp Val Glu Gln 
        515                 520                 525             


Leu Glu Asp Arg Ala Gly Ala Gly Ile Ile Glu Thr Pro Arg Gly Ala 
    530                 535                 540                 


Ile Lys Val Thr Ala Gln Pro Thr Asp His Val Val Gly Glu Tyr Leu 
545                 550                 555                 560 


Val Leu Ser Pro Gln Thr Val Leu Arg Ser Gln Lys Leu Ser Leu Ile 
                565                 570                 575     


His Ala Leu Ala Glu Gln Val Lys Thr Cys Thr His Asn Gly Arg Ala 
            580                 585                 590         


Gly Arg Tyr Ala Val Glu Ala Tyr Asp Gly Arg Val Leu Val Pro Ser 
        595                 600                 605             


Gly Tyr Ala Ile Ser Pro Glu Asp Phe Gln Ser Leu Ser Glu Ser Ala 
    610                 615                 620                 


Thr Met Val Tyr Asn Glu Arg Glu Phe Val Asn Arg Lys Leu His His 
625                 630                 635                 640 


Ile Ala Met His Gly Pro Ala Leu Asn Thr Asp Glu Glu Ser Tyr Glu 
                645                 650                 655     


Leu Val Arg Ala Glu Arg Thr Glu His Glu Tyr Val Tyr Asp Val Asp 
            660                 665                 670         


Gln Arg Arg Cys Cys Lys Lys Glu Glu Ala Ala Gly Leu Val Leu Val 
        675                 680                 685             


Gly Asp Leu Thr Asn Pro Pro Tyr His Glu Phe Ala Tyr Glu Gly Leu 
    690                 695                 700                 


Lys Ile Arg Pro Ala Cys Pro Tyr Lys Ile Ala Val Ile Gly Val Phe 
705                 710                 715                 720 


Gly Val Pro Gly Ser Gly Lys Ser Ala Ile Ile Lys Asn Leu Val Thr 
                725                 730                 735     


Arg Gln Asp Leu Val Thr Ser Gly Lys Lys Glu Asn Cys Gln Glu Ile 
            740                 745                 750         


Thr Thr Asp Val Met Arg Gln Arg Gly Leu Glu Ile Ser Ala Arg Thr 
        755                 760                 765             


Val Asp Ser Leu Leu Leu Asn Gly Cys Asn Arg Pro Val Asp Val Leu 
    770                 775                 780                 


Tyr Val Asp Glu Ala Phe Ala Cys His Ser Gly Thr Leu Leu Ala Leu 
785                 790                 795                 800 


Ile Ala Leu Val Arg Pro Arg Gln Lys Val Val Leu Cys Gly Asp Pro 
                805                 810                 815     


Lys Gln Cys Gly Phe Phe Asn Met Met Gln Met Lys Val Asn Tyr Asn 
            820                 825                 830         


His Asn Ile Cys Thr Gln Val Tyr His Lys Ser Ile Ser Arg Arg Cys 
        835                 840                 845             


Thr Leu Pro Val Thr Ala Ile Val Ser Ser Leu His Tyr Glu Gly Lys 
    850                 855                 860                 


Met Arg Thr Thr Asn Glu Tyr Asn Lys Pro Ile Val Val Asp Thr Thr 
865                 870                 875                 880 


Gly Ser Thr Lys Pro Asp Pro Gly Asp Leu Val Leu Thr Cys Phe Arg 
                885                 890                 895     


Gly Trp Val Lys Gln Leu Gln Ile Asp Tyr Arg Gly Tyr Glu Val Met 
            900                 905                 910         


Thr Ala Ala Ala Ser Gln Gly Leu Thr Arg Lys Gly Val Tyr Ala Val 
        915                 920                 925             


Arg Gln Lys Val Asn Glu Asn Pro Leu Tyr Ala Ser Thr Ser Glu His 
    930                 935                 940                 


Val Asn Val Leu Leu Thr Arg Thr Glu Gly Lys Leu Val Trp Lys Thr 
945                 950                 955                 960 


Leu Ser Gly Asp Pro Trp Ile Lys Thr Leu Gln Asn Pro Pro Lys Gly 
                965                 970                 975     


Asn Phe Lys Ala Thr Ile Lys Glu Trp Glu Val Glu His Ala Ser Ile 
            980                 985                 990         


Met Ala Gly Ile Cys Ser His Gln  Met Thr Phe Asp Thr  Phe Gln Asn 
        995                 1000                 1005             


Lys Ala  Asn Val Cys Trp Ala  Lys Ser Leu Val Pro  Ile Leu Glu 
    1010                 1015                 1020             


Thr Ala  Gly Ile Lys Leu Asn  Asp Arg Gln Trp Ser  Gln Ile Ile 
    1025                 1030                 1035             


Gln Ala  Phe Lys Glu Asp Lys  Ala Tyr Ser Pro Glu  Val Ala Leu 
    1040                 1045                 1050             


Asn Glu  Ile Cys Thr Arg Met  Tyr Gly Val Asp Leu  Asp Ser Gly 
    1055                 1060                 1065             


Leu Phe  Ser Lys Pro Leu Val  Ser Val Tyr Tyr Ala  Asp Asn His 
    1070                 1075                 1080             


Trp Asp  Asn Arg Pro Gly Gly  Lys Met Phe Gly Phe  Asn Pro Glu 
    1085                 1090                 1095             


Ala Ala  Ser Ile Leu Glu Arg  Lys Tyr Pro Phe Thr  Lys Gly Lys 
    1100                 1105                 1110             


Trp Asn  Ile Asn Lys Gln Ile  Cys Val Thr Thr Arg  Arg Ile Glu 
    1115                 1120                 1125             


Asp Phe  Asn Pro Thr Thr Asn  Ile Ile Pro Ala Asn  Arg Arg Leu 
    1130                 1135                 1140             


Pro His  Ser Leu Val Ala Glu  His Arg Pro Val Lys  Gly Glu Arg 
    1145                 1150                 1155             


Met Glu  Trp Leu Val Asn Lys  Ile Asn Gly His His  Val Leu Leu 
    1160                 1165                 1170             


Val Ser  Gly Tyr Asn Leu Ala  Leu Pro Thr Lys Arg  Val Thr Trp 
    1175                 1180                 1185             


Val Ala  Pro Leu Gly Val Arg  Gly Ala Asp Tyr Thr  Tyr Asn Leu 
    1190                 1195                 1200             


Glu Leu  Gly Leu Pro Ala Thr  Leu Gly Arg Tyr Asp  Leu Val Val 
    1205                 1210                 1215             


Ile Asn  Ile His Thr Pro Phe  Arg Ile His His Tyr  Gln Gln Cys 
    1220                 1225                 1230             


Val Asp  His Ala Met Lys Leu  Gln Met Leu Gly Gly  Asp Ser Leu 
    1235                 1240                 1245             


Arg Leu  Leu Lys Pro Gly Gly  Ser Leu Leu Ile Arg  Ala Tyr Gly 
    1250                 1255                 1260             


Tyr Ala  Asp Arg Thr Ser Glu  Arg Val Ile Cys Val  Leu Gly Arg 
    1265                 1270                 1275             


Lys Phe  Arg Ser Ser Arg Ala  Leu Lys Pro Pro Cys  Val Thr Ser 
    1280                 1285                 1290             


Asn Thr  Glu Met Phe Phe Leu  Phe Ser Asn Phe Asp  Asn Gly Arg 
    1295                 1300                 1305             


Arg Asn  Phe Thr Thr His Val  Met Asn Asn Gln Leu  Asn Ala Ala 
    1310                 1315                 1320             


Phe Val  Gly Gln Val Thr Arg  Ala Gly Cys Ala Pro  Ser Tyr Arg 
    1325                 1330                 1335             


Val Lys  Arg Met Asp Ile Ala  Lys Asn Asp Glu Glu  Cys Val Val 
    1340                 1345                 1350             


Asn Ala  Ala Asn Pro Arg Gly  Leu Pro Gly Gly Gly  Val Cys Lys 
    1355                 1360                 1365             


Ala Val  Tyr Lys Lys Trp Pro  Glu Ser Phe Lys Asn  Ser Ala Thr 
    1370                 1375                 1380             


Pro Val  Gly Thr Ala Lys Thr  Val Met Cys Gly Thr  Tyr Pro Val 
    1385                 1390                 1395             


Ile His  Ala Val Gly Pro Asn  Phe Ser Asn Tyr Ser  Glu Ser Glu 
    1400                 1405                 1410             


Gly Asp  Arg Glu Leu Ala Ala  Ala Tyr Arg Glu Val  Ala Lys Glu 
    1415                 1420                 1425             


Val Thr  Arg Leu Gly Val Asn  Ser Val Ala Ile Pro  Leu Leu Ser 
    1430                 1435                 1440             


Thr Gly  Val Tyr Ser Gly Gly  Lys Asp Arg Leu Thr  Gln Ser Leu 
    1445                 1450                 1455             


Asn His  Leu Phe Thr Ala Met  Asp Ser Thr Asp Ala  Asp Val Val 
    1460                 1465                 1470             


Ile Tyr  Cys Arg Asp Lys Glu  Trp Glu Lys Lys Ile  Ser Glu Ala 
    1475                 1480                 1485             


Ile Gln  Met Arg Thr Gln Val  Glu Leu Leu Asp Glu  His Ile Ser 
    1490                 1495                 1500             


Ile Asp  Cys Asp Ile Val Arg  Val His Pro Asp Ser  Ser Leu Ala 
    1505                 1510                 1515             


Gly Arg  Lys Gly Tyr Ser Thr  Thr Glu Gly Ala Leu  Tyr Ser Tyr 
    1520                 1525                 1530             


Leu Glu  Gly Thr Arg Phe His  Gln Thr Ala Val Asp  Met Ala Glu 
    1535                 1540                 1545             


Ile His  Thr Met Trp Pro Lys  Gln Thr Glu Ala Asn  Glu Gln Val 
    1550                 1555                 1560             


Cys Leu  Tyr Ala Leu Gly Glu  Ser Ile Glu Ser Ile  Arg Gln Lys 
    1565                 1570                 1575             


Cys Pro  Val Asp Asp Ala Asp  Ala Ser Ser Pro Pro  Lys Thr Val 
    1580                 1585                 1590             


Pro Cys  Leu Cys Arg Tyr Ala  Met Thr Pro Glu Arg  Val Thr Arg 
    1595                 1600                 1605             


Leu Arg  Met Asn His Val Thr  Ser Ile Ile Val Cys  Ser Ser Phe 
    1610                 1615                 1620             


Pro Leu  Pro Lys Tyr Lys Ile  Glu Gly Val Gln Lys  Val Lys Cys 
    1625                 1630                 1635             


Ser Lys  Val Met Leu Phe Asp  His Asn Val Pro Ser  Arg Val Ser 
    1640                 1645                 1650             


Pro Arg  Glu Tyr Arg Ser Ser  Gln Glu Ser Ala Gln  Glu Ala Ser 
    1655                 1660                 1665             


Thr Ile  Thr Ser Leu Thr His  Ser Gln Phe Asp Leu  Ser Val Asp 
    1670                 1675                 1680             


Gly Glu  Ile Leu Pro Val Pro  Ser Asp Leu Asp Ala  Asp Ala Pro 
    1685                 1690                 1695             


Ala Leu  Glu Pro Ala Leu Asp  Asp Gly Ala Thr His  Thr Leu Pro 
    1700                 1705                 1710             


Ser Thr  Thr Gly Asn Leu Ala  Ala Val Ser Asp Trp  Val Met Ser 
    1715                 1720                 1725             


Thr Val  Pro Val Ala Pro Pro  Arg Arg Arg Arg Gly  Arg Asn Leu 
    1730                 1735                 1740             


Thr Val  Thr Cys Asp Glu Arg  Glu Gly Asn Ile Thr  Pro Met Ala 
    1745                 1750                 1755             


Ser Val  Arg Phe Phe Arg Ala  Glu Leu Cys Pro Val  Val Gln Glu 
    1760                 1765                 1770             


Thr Ala  Glu Thr Arg Asp Thr  Ala Met Ser Leu Gln  Ala Pro Pro 
    1775                 1780                 1785             


Ser Thr  Ala Thr Glu Pro Asn  His Pro Pro Ile Ser  Phe Gly Ala 
    1790                 1795                 1800             


Ser Ser  Glu Thr Phe Pro Ile  Thr Phe Gly Asp Phe  Asn Glu Gly 
    1805                 1810                 1815             


Glu Ile  Glu Ser Leu Ser Ser  Glu Leu Leu Thr Phe  Gly Asp Phe 
    1820                 1825                 1830             


Leu Pro  Gly Glu Val Asp Asp  Leu Thr Asp Ser Asp  Trp Ser Thr 
    1835                 1840                 1845             


Cys Ser  Asp Thr Asp Asp Glu  Leu Arg Leu Asp Arg  Ala Gly Gly 
    1850                 1855                 1860             


Tyr Ile  Phe Ser Ser Asp Thr  Gly Pro Gly His Leu  Gln Gln Lys 
    1865                 1870                 1875             


Ser Val  Arg Gln Ser Val Leu  Pro Val Asn Thr Leu  Glu Glu Val 
    1880                 1885                 1890             


His Glu  Glu Lys Cys Tyr Pro  Pro Lys Leu Asp Glu  Ala Lys Glu 
    1895                 1900                 1905             


Gln Leu  Leu Leu Lys Lys Leu  Gln Glu Ser Ala Ser  Met Ala Asn 
    1910                 1915                 1920             


Arg Ser  Arg Tyr Gln Ser Arg  Lys Val Glu Asn Met  Lys Ala Ala 
    1925                 1930                 1935             


Ile Ile  Gln Arg Leu Lys Arg  Gly Cys Arg Leu Tyr  Leu Met Ser 
    1940                 1945                 1950             


Glu Thr  Pro Lys Val Pro Thr  Tyr Arg Thr Thr Tyr  Pro Ala Pro 
    1955                 1960                 1965             


Val Tyr  Ser Pro Pro Ile Asn  Val Arg Leu Ser Asn  Pro Glu Ser 
    1970                 1975                 1980             


Ala Val  Ala Ala Cys Asn Glu  Phe Leu Ala Arg Asn  Tyr Pro Thr 
    1985                 1990                 1995             


Val Ser  Ser Tyr Gln Ile Thr  Asp Glu Tyr Asp Ala  Tyr Leu Asp 
    2000                 2005                 2010             


Met Val  Asp Gly Ser Glu Ser  Cys Leu Asp Arg Ala  Thr Phe Asn 
    2015                 2020                 2025             


Pro Ser  Lys Leu Arg Ser Tyr  Pro Lys Gln His Ala  Tyr His Ala 
    2030                 2035                 2040             


Pro Ser  Ile Arg Ser Ala Val  Pro Ser Pro Phe Gln  Asn Thr Leu 
    2045                 2050                 2055             


Gln Asn  Val Leu Ala Ala Ala  Thr Lys Arg Asn Cys  Asn Val Thr 
    2060                 2065                 2070             


Gln Met  Arg Glu Leu Pro Thr  Leu Asp Ser Ala Val  Phe Asn Val 
    2075                 2080                 2085             


Glu Cys  Phe Lys Lys Phe Ala  Cys Asn Gln Glu Tyr  Trp Glu Glu 
    2090                 2095                 2100             


Phe Ala  Ala Ser Pro Ile Arg  Ile Thr Thr Glu Asn  Leu Ala Thr 
    2105                 2110                 2115             


Tyr Val  Thr Lys Leu Lys Gly  Pro Lys Ala Ala Ala  Leu Phe Ala 
    2120                 2125                 2130             


Lys Thr  His Asn Leu Leu Pro  Leu Gln Glu Val Pro  Met Asp Arg 
    2135                 2140                 2145             


Phe Thr  Val Asp Met Lys Arg  Asp Val Lys Val Thr  Pro Gly Thr 
    2150                 2155                 2160             


Lys His  Thr Glu Glu Arg Pro  Lys Val Gln Val Ile  Gln Ala Ala 
    2165                 2170                 2175             


Glu Pro  Leu Ala Thr Ala Tyr  Leu Cys Gly Ile His  Arg Glu Leu 
    2180                 2185                 2190             


Val Arg  Arg Leu Asn Ala Val  Leu Leu Pro Asn Val  His Thr Leu 
    2195                 2200                 2205             


Phe Asp  Met Ser Ala Glu Asp  Phe Asp Ala Ile Ile  Ala Ala His 
    2210                 2215                 2220             


Phe Lys  Pro Gly Asp Thr Val  Leu Glu Thr Asp Ile  Ala Ser Phe 
    2225                 2230                 2235             


Asp Lys  Ser Gln Asp Asp Ser  Leu Ala Leu Thr Ala  Leu Met Leu 
    2240                 2245                 2250             


Leu Glu  Asp Leu Gly Val Asp  His Ser Leu Leu Asp  Leu Ile Glu 
    2255                 2260                 2265             


Ala Ala  Phe Gly Glu Ile Ser  Ser Cys His Leu Pro  Thr Gly Thr 
    2270                 2275                 2280             


Arg Phe  Lys Phe Gly Ala Met  Met Lys Ser Gly Met  Phe Leu Thr 
    2285                 2290                 2295             


Leu Phe  Val Asn Thr Leu Leu  Asn Ile Thr Ile Ala  Ser Arg Val 
    2300                 2305                 2310             


Leu Glu  Asp Arg Leu Thr Lys  Ser Ala Cys Ala Ala  Phe Ile Gly 
    2315                 2320                 2325             


Asp Asp  Asn Ile Ile His Gly  Val Val Ser Asp Glu  Leu Met Ala 
    2330                 2335                 2340             


Ala Arg  Cys Ala Thr Trp Met  Asn Met Glu Val Lys  Ile Ile Asp 
    2345                 2350                 2355             


Ala Val  Val Ser Leu Lys Ala  Pro Tyr Phe Cys Gly  Gly Phe Ile 
    2360                 2365                 2370             


Leu His  Asp Thr Val Thr Gly  Thr Ala Cys Arg Val  Ala Asp Pro 
    2375                 2380                 2385             


Leu Lys  Arg Leu Phe Lys Leu  Gly Lys Pro Leu Ala  Ala Gly Asp 
    2390                 2395                 2400             


Glu Gln  Asp Glu Asp Arg Arg  Arg Ala Leu Ala Asp  Glu Val Ile 
    2405                 2410                 2415             


Arg Trp  Gln Arg Thr Gly Leu  Ile Asp Glu Leu Glu  Lys Ala Val 
    2420                 2425                 2430             


Tyr Ser  Arg Tyr Glu Val Gln  Gly Ile Ser Val Val  Val Met Ser 
    2435                 2440                 2445             


Met Ala  Thr Phe Ala Ser Ser  Arg Ser Asn Phe Glu  Lys Leu Arg 
    2450                 2455                 2460             


Gly Pro  Val Ile Thr Leu Tyr  Gly Gly Pro Lys 
    2465                 2470                 


<210>  2
<211>  2474
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide sequence of CHIKV LR2006 OPY1 non-structural 
       polyprotein with mutation at position 532 from arginine to 
       histidine.

<400>  2

Met Asp Pro Val Tyr Val Asp Ile Asp Ala Asp Ser Ala Phe Leu Lys 
1               5                   10                  15      


Ala Leu Gln Arg Ala Tyr Pro Met Phe Glu Val Glu Pro Arg Gln Val 
            20                  25                  30          


Thr Pro Asn Asp His Ala Asn Ala Arg Ala Phe Ser His Leu Ala Ile 
        35                  40                  45              


Lys Leu Ile Glu Gln Glu Ile Asp Pro Asp Ser Thr Ile Leu Asp Ile 
    50                  55                  60                  


Gly Ser Ala Pro Ala Arg Arg Met Met Ser Asp Arg Lys Tyr His Cys 
65                  70                  75                  80  


Val Cys Pro Met Arg Ser Ala Glu Asp Pro Glu Arg Leu Ala Asn Tyr 
                85                  90                  95      


Ala Arg Lys Leu Ala Ser Ala Ala Gly Lys Val Leu Asp Arg Asn Ile 
            100                 105                 110         


Ser Gly Lys Ile Gly Asp Leu Gln Ala Val Met Ala Val Pro Asp Thr 
        115                 120                 125             


Glu Thr Pro Thr Phe Cys Leu His Thr Asp Val Ser Cys Arg Gln Arg 
    130                 135                 140                 


Ala Asp Val Ala Ile Tyr Gln Asp Val Tyr Ala Val His Ala Pro Thr 
145                 150                 155                 160 


Ser Leu Tyr His Gln Ala Ile Lys Gly Val Arg Val Ala Tyr Trp Val 
                165                 170                 175     


Gly Phe Asp Thr Thr Pro Phe Met Tyr Asn Ala Met Ala Gly Ala Tyr 
            180                 185                 190         


Pro Ser Tyr Ser Thr Asn Trp Ala Asp Glu Gln Val Leu Lys Ala Lys 
        195                 200                 205             


Asn Ile Gly Leu Cys Ser Thr Asp Leu Thr Glu Gly Arg Arg Gly Lys 
    210                 215                 220                 


Leu Ser Ile Met Arg Gly Lys Lys Leu Lys Pro Cys Asp Arg Val Leu 
225                 230                 235                 240 


Phe Ser Val Gly Ser Thr Leu Tyr Pro Glu Ser Arg Lys Leu Leu Lys 
                245                 250                 255     


Ser Trp His Leu Pro Ser Val Phe His Leu Lys Gly Lys Leu Ser Phe 
            260                 265                 270         


Thr Cys Arg Cys Asp Thr Val Val Ser Cys Glu Gly Tyr Val Val Lys 
        275                 280                 285             


Arg Ile Thr Met Ser Pro Gly Leu Tyr Gly Lys Thr Thr Gly Tyr Ala 
    290                 295                 300                 


Val Thr His His Ala Asp Gly Phe Leu Met Cys Lys Thr Thr Asp Thr 
305                 310                 315                 320 


Val Asp Gly Glu Arg Met Ser Phe Ser Val Cys Thr Tyr Val Pro Ala 
                325                 330                 335     


Thr Ile Cys Asp Gln Met Thr Gly Ile Leu Ala Thr Glu Val Thr Pro 
            340                 345                 350         


Glu Asp Ala Gln Lys Leu Leu Val Gly Leu Asn Gln Arg Ile Val Val 
        355                 360                 365             


Asn Gly Arg Thr Gln Arg Asn Thr Asn Thr Met Lys Asn Tyr Leu Leu 
    370                 375                 380                 


Pro Val Val Ala Gln Ala Phe Ser Lys Trp Ala Lys Glu Cys Arg Lys 
385                 390                 395                 400 


Asp Met Glu Asp Glu Lys Leu Leu Gly Val Arg Glu Arg Thr Leu Thr 
                405                 410                 415     


Cys Cys Cys Leu Trp Ala Phe Lys Lys Gln Lys Thr His Thr Val Tyr 
            420                 425                 430         


Lys Arg Pro Asp Thr Gln Ser Ile Gln Lys Val Gln Ala Glu Phe Asp 
        435                 440                 445             


Ser Phe Val Val Pro Ser Leu Trp Ser Ser Gly Leu Ser Ile Pro Leu 
    450                 455                 460                 


Arg Thr Arg Ile Lys Trp Leu Leu Ser Lys Val Pro Lys Thr Asp Leu 
465                 470                 475                 480 


Ile Pro Tyr Ser Gly Asp Ala Arg Glu Ala Arg Asp Ala Glu Lys Glu 
                485                 490                 495     


Ala Glu Glu Glu Arg Glu Ala Glu Leu Thr Arg Glu Ala Leu Pro Pro 
            500                 505                 510         


Leu Gln Ala Ala Gln Glu Asp Val Gln Val Glu Ile Asp Val Glu Gln 
        515                 520                 525             


Leu Glu Asp His Ala Gly Ala Gly Ile Ile Glu Thr Pro Arg Gly Ala 
    530                 535                 540                 


Ile Lys Val Thr Ala Gln Pro Thr Asp His Val Val Gly Glu Tyr Leu 
545                 550                 555                 560 


Val Leu Ser Pro Gln Thr Val Leu Arg Ser Gln Lys Leu Ser Leu Ile 
                565                 570                 575     


His Ala Leu Ala Glu Gln Val Lys Thr Cys Thr His Asn Gly Arg Ala 
            580                 585                 590         


Gly Arg Tyr Ala Val Glu Ala Tyr Asp Gly Arg Val Leu Val Pro Ser 
        595                 600                 605             


Gly Tyr Ala Ile Ser Pro Glu Asp Phe Gln Ser Leu Ser Glu Ser Ala 
    610                 615                 620                 


Thr Met Val Tyr Asn Glu Arg Glu Phe Val Asn Arg Lys Leu His His 
625                 630                 635                 640 


Ile Ala Met His Gly Pro Ala Leu Asn Thr Asp Glu Glu Ser Tyr Glu 
                645                 650                 655     


Leu Val Arg Ala Glu Arg Thr Glu His Glu Tyr Val Tyr Asp Val Asp 
            660                 665                 670         


Gln Arg Arg Cys Cys Lys Lys Glu Glu Ala Ala Gly Leu Val Leu Val 
        675                 680                 685             


Gly Asp Leu Thr Asn Pro Pro Tyr His Glu Phe Ala Tyr Glu Gly Leu 
    690                 695                 700                 


Lys Ile Arg Pro Ala Cys Pro Tyr Lys Ile Ala Val Ile Gly Val Phe 
705                 710                 715                 720 


Gly Val Pro Gly Ser Gly Lys Ser Ala Ile Ile Lys Asn Leu Val Thr 
                725                 730                 735     


Arg Gln Asp Leu Val Thr Ser Gly Lys Lys Glu Asn Cys Gln Glu Ile 
            740                 745                 750         


Thr Thr Asp Val Met Arg Gln Arg Gly Leu Glu Ile Ser Ala Arg Thr 
        755                 760                 765             


Val Asp Ser Leu Leu Leu Asn Gly Cys Asn Arg Pro Val Asp Val Leu 
    770                 775                 780                 


Tyr Val Asp Glu Ala Phe Ala Cys His Ser Gly Thr Leu Leu Ala Leu 
785                 790                 795                 800 


Ile Ala Leu Val Arg Pro Arg Gln Lys Val Val Leu Cys Gly Asp Pro 
                805                 810                 815     


Lys Gln Cys Gly Phe Phe Asn Met Met Gln Met Lys Val Asn Tyr Asn 
            820                 825                 830         


His Asn Ile Cys Thr Gln Val Tyr His Lys Ser Ile Ser Arg Arg Cys 
        835                 840                 845             


Thr Leu Pro Val Thr Ala Ile Val Ser Ser Leu His Tyr Glu Gly Lys 
    850                 855                 860                 


Met Arg Thr Thr Asn Glu Tyr Asn Lys Pro Ile Val Val Asp Thr Thr 
865                 870                 875                 880 


Gly Ser Thr Lys Pro Asp Pro Gly Asp Leu Val Leu Thr Cys Phe Arg 
                885                 890                 895     


Gly Trp Val Lys Gln Leu Gln Ile Asp Tyr Arg Gly Tyr Glu Val Met 
            900                 905                 910         


Thr Ala Ala Ala Ser Gln Gly Leu Thr Arg Lys Gly Val Tyr Ala Val 
        915                 920                 925             


Arg Gln Lys Val Asn Glu Asn Pro Leu Tyr Ala Ser Thr Ser Glu His 
    930                 935                 940                 


Val Asn Val Leu Leu Thr Arg Thr Glu Gly Lys Leu Val Trp Lys Thr 
945                 950                 955                 960 


Leu Ser Gly Asp Pro Trp Ile Lys Thr Leu Gln Asn Pro Pro Lys Gly 
                965                 970                 975     


Asn Phe Lys Ala Thr Ile Lys Glu Trp Glu Val Glu His Ala Ser Ile 
            980                 985                 990         


Met Ala Gly Ile Cys Ser His Gln  Met Thr Phe Asp Thr  Phe Gln Asn 
        995                 1000                 1005             


Lys Ala  Asn Val Cys Trp Ala  Lys Ser Leu Val Pro  Ile Leu Glu 
    1010                 1015                 1020             


Thr Ala  Gly Ile Lys Leu Asn  Asp Arg Gln Trp Ser  Gln Ile Ile 
    1025                 1030                 1035             


Gln Ala  Phe Lys Glu Asp Lys  Ala Tyr Ser Pro Glu  Val Ala Leu 
    1040                 1045                 1050             


Asn Glu  Ile Cys Thr Arg Met  Tyr Gly Val Asp Leu  Asp Ser Gly 
    1055                 1060                 1065             


Leu Phe  Ser Lys Pro Leu Val  Ser Val Tyr Tyr Ala  Asp Asn His 
    1070                 1075                 1080             


Trp Asp  Asn Arg Pro Gly Gly  Lys Met Phe Gly Phe  Asn Pro Glu 
    1085                 1090                 1095             


Ala Ala  Ser Ile Leu Glu Arg  Lys Tyr Pro Phe Thr  Lys Gly Lys 
    1100                 1105                 1110             


Trp Asn  Ile Asn Lys Gln Ile  Cys Val Thr Thr Arg  Arg Ile Glu 
    1115                 1120                 1125             


Asp Phe  Asn Pro Thr Thr Asn  Ile Ile Pro Ala Asn  Arg Arg Leu 
    1130                 1135                 1140             


Pro His  Ser Leu Val Ala Glu  His Arg Pro Val Lys  Gly Glu Arg 
    1145                 1150                 1155             


Met Glu  Trp Leu Val Asn Lys  Ile Asn Gly His His  Val Leu Leu 
    1160                 1165                 1170             


Val Ser  Gly Tyr Asn Leu Ala  Leu Pro Thr Lys Arg  Val Thr Trp 
    1175                 1180                 1185             


Val Ala  Pro Leu Gly Val Arg  Gly Ala Asp Tyr Thr  Tyr Asn Leu 
    1190                 1195                 1200             


Glu Leu  Gly Leu Pro Ala Thr  Leu Gly Arg Tyr Asp  Leu Val Val 
    1205                 1210                 1215             


Ile Asn  Ile His Thr Pro Phe  Arg Ile His His Tyr  Gln Gln Cys 
    1220                 1225                 1230             


Val Asp  His Ala Met Lys Leu  Gln Met Leu Gly Gly  Asp Ser Leu 
    1235                 1240                 1245             


Arg Leu  Leu Lys Pro Gly Gly  Ser Leu Leu Ile Arg  Ala Tyr Gly 
    1250                 1255                 1260             


Tyr Ala  Asp Arg Thr Ser Glu  Arg Val Ile Cys Val  Leu Gly Arg 
    1265                 1270                 1275             


Lys Phe  Arg Ser Ser Arg Ala  Leu Lys Pro Pro Cys  Val Thr Ser 
    1280                 1285                 1290             


Asn Thr  Glu Met Phe Phe Leu  Phe Ser Asn Phe Asp  Asn Gly Arg 
    1295                 1300                 1305             


Arg Asn  Phe Thr Thr His Val  Met Asn Asn Gln Leu  Asn Ala Ala 
    1310                 1315                 1320             


Phe Val  Gly Gln Val Thr Arg  Ala Gly Cys Ala Pro  Ser Tyr Arg 
    1325                 1330                 1335             


Val Lys  Arg Met Asp Ile Ala  Lys Asn Asp Glu Glu  Cys Val Val 
    1340                 1345                 1350             


Asn Ala  Ala Asn Pro Arg Gly  Leu Pro Gly Gly Gly  Val Cys Lys 
    1355                 1360                 1365             


Ala Val  Tyr Lys Lys Trp Pro  Glu Ser Phe Lys Asn  Ser Ala Thr 
    1370                 1375                 1380             


Pro Val  Gly Thr Ala Lys Thr  Val Met Cys Gly Thr  Tyr Pro Val 
    1385                 1390                 1395             


Ile His  Ala Val Gly Pro Asn  Phe Ser Asn Tyr Ser  Glu Ser Glu 
    1400                 1405                 1410             


Gly Asp  Arg Glu Leu Ala Ala  Ala Tyr Arg Glu Val  Ala Lys Glu 
    1415                 1420                 1425             


Val Thr  Arg Leu Gly Val Asn  Ser Val Ala Ile Pro  Leu Leu Ser 
    1430                 1435                 1440             


Thr Gly  Val Tyr Ser Gly Gly  Lys Asp Arg Leu Thr  Gln Ser Leu 
    1445                 1450                 1455             


Asn His  Leu Phe Thr Ala Met  Asp Ser Thr Asp Ala  Asp Val Val 
    1460                 1465                 1470             


Ile Tyr  Cys Arg Asp Lys Glu  Trp Glu Lys Lys Ile  Ser Glu Ala 
    1475                 1480                 1485             


Ile Gln  Met Arg Thr Gln Val  Glu Leu Leu Asp Glu  His Ile Ser 
    1490                 1495                 1500             


Ile Asp  Cys Asp Ile Val Arg  Val His Pro Asp Ser  Ser Leu Ala 
    1505                 1510                 1515             


Gly Arg  Lys Gly Tyr Ser Thr  Thr Glu Gly Ala Leu  Tyr Ser Tyr 
    1520                 1525                 1530             


Leu Glu  Gly Thr Arg Phe His  Gln Thr Ala Val Asp  Met Ala Glu 
    1535                 1540                 1545             


Ile His  Thr Met Trp Pro Lys  Gln Thr Glu Ala Asn  Glu Gln Val 
    1550                 1555                 1560             


Cys Leu  Tyr Ala Leu Gly Glu  Ser Ile Glu Ser Ile  Arg Gln Lys 
    1565                 1570                 1575             


Cys Pro  Val Asp Asp Ala Asp  Ala Ser Ser Pro Pro  Lys Thr Val 
    1580                 1585                 1590             


Pro Cys  Leu Cys Arg Tyr Ala  Met Thr Pro Glu Arg  Val Thr Arg 
    1595                 1600                 1605             


Leu Arg  Met Asn His Val Thr  Ser Ile Ile Val Cys  Ser Ser Phe 
    1610                 1615                 1620             


Pro Leu  Pro Lys Tyr Lys Ile  Glu Gly Val Gln Lys  Val Lys Cys 
    1625                 1630                 1635             


Ser Lys  Val Met Leu Phe Asp  His Asn Val Pro Ser  Arg Val Ser 
    1640                 1645                 1650             


Pro Arg  Glu Tyr Arg Ser Ser  Gln Glu Ser Ala Gln  Glu Ala Ser 
    1655                 1660                 1665             


Thr Ile  Thr Ser Leu Thr His  Ser Gln Phe Asp Leu  Ser Val Asp 
    1670                 1675                 1680             


Gly Glu  Ile Leu Pro Val Pro  Ser Asp Leu Asp Ala  Asp Ala Pro 
    1685                 1690                 1695             


Ala Leu  Glu Pro Ala Leu Asp  Asp Gly Ala Thr His  Thr Leu Pro 
    1700                 1705                 1710             


Ser Thr  Thr Gly Asn Leu Ala  Ala Val Ser Asp Trp  Val Met Ser 
    1715                 1720                 1725             


Thr Val  Pro Val Ala Pro Pro  Arg Arg Arg Arg Gly  Arg Asn Leu 
    1730                 1735                 1740             


Thr Val  Thr Cys Asp Glu Arg  Glu Gly Asn Ile Thr  Pro Met Ala 
    1745                 1750                 1755             


Ser Val  Arg Phe Phe Arg Ala  Glu Leu Cys Pro Val  Val Gln Glu 
    1760                 1765                 1770             


Thr Ala  Glu Thr Arg Asp Thr  Ala Met Ser Leu Gln  Ala Pro Pro 
    1775                 1780                 1785             


Ser Thr  Ala Thr Glu Pro Asn  His Pro Pro Ile Ser  Phe Gly Ala 
    1790                 1795                 1800             


Ser Ser  Glu Thr Phe Pro Ile  Thr Phe Gly Asp Phe  Asn Glu Gly 
    1805                 1810                 1815             


Glu Ile  Glu Ser Leu Ser Ser  Glu Leu Leu Thr Phe  Gly Asp Phe 
    1820                 1825                 1830             


Leu Pro  Gly Glu Val Asp Asp  Leu Thr Asp Ser Asp  Trp Ser Thr 
    1835                 1840                 1845             


Cys Ser  Asp Thr Asp Asp Glu  Leu Arg Leu Asp Arg  Ala Gly Gly 
    1850                 1855                 1860             


Tyr Ile  Phe Ser Ser Asp Thr  Gly Pro Gly His Leu  Gln Gln Lys 
    1865                 1870                 1875             


Ser Val  Arg Gln Ser Val Leu  Pro Val Asn Thr Leu  Glu Glu Val 
    1880                 1885                 1890             


His Glu  Glu Lys Cys Tyr Pro  Pro Lys Leu Asp Glu  Ala Lys Glu 
    1895                 1900                 1905             


Gln Leu  Leu Leu Lys Lys Leu  Gln Glu Ser Ala Ser  Met Ala Asn 
    1910                 1915                 1920             


Arg Ser  Arg Tyr Gln Ser Arg  Lys Val Glu Asn Met  Lys Ala Ala 
    1925                 1930                 1935             


Ile Ile  Gln Arg Leu Lys Arg  Gly Cys Arg Leu Tyr  Leu Met Ser 
    1940                 1945                 1950             


Glu Thr  Pro Lys Val Pro Thr  Tyr Arg Thr Thr Tyr  Pro Ala Pro 
    1955                 1960                 1965             


Val Tyr  Ser Pro Pro Ile Asn  Val Arg Leu Ser Asn  Pro Glu Ser 
    1970                 1975                 1980             


Ala Val  Ala Ala Cys Asn Glu  Phe Leu Ala Arg Asn  Tyr Pro Thr 
    1985                 1990                 1995             


Val Ser  Ser Tyr Gln Ile Thr  Asp Glu Tyr Asp Ala  Tyr Leu Asp 
    2000                 2005                 2010             


Met Val  Asp Gly Ser Glu Ser  Cys Leu Asp Arg Ala  Thr Phe Asn 
    2015                 2020                 2025             


Pro Ser  Lys Leu Arg Ser Tyr  Pro Lys Gln His Ala  Tyr His Ala 
    2030                 2035                 2040             


Pro Ser  Ile Arg Ser Ala Val  Pro Ser Pro Phe Gln  Asn Thr Leu 
    2045                 2050                 2055             


Gln Asn  Val Leu Ala Ala Ala  Thr Lys Arg Asn Cys  Asn Val Thr 
    2060                 2065                 2070             


Gln Met  Arg Glu Leu Pro Thr  Leu Asp Ser Ala Val  Phe Asn Val 
    2075                 2080                 2085             


Glu Cys  Phe Lys Lys Phe Ala  Cys Asn Gln Glu Tyr  Trp Glu Glu 
    2090                 2095                 2100             


Phe Ala  Ala Ser Pro Ile Arg  Ile Thr Thr Glu Asn  Leu Ala Thr 
    2105                 2110                 2115             


Tyr Val  Thr Lys Leu Lys Gly  Pro Lys Ala Ala Ala  Leu Phe Ala 
    2120                 2125                 2130             


Lys Thr  His Asn Leu Leu Pro  Leu Gln Glu Val Pro  Met Asp Arg 
    2135                 2140                 2145             


Phe Thr  Val Asp Met Lys Arg  Asp Val Lys Val Thr  Pro Gly Thr 
    2150                 2155                 2160             


Lys His  Thr Glu Glu Arg Pro  Lys Val Gln Val Ile  Gln Ala Ala 
    2165                 2170                 2175             


Glu Pro  Leu Ala Thr Ala Tyr  Leu Cys Gly Ile His  Arg Glu Leu 
    2180                 2185                 2190             


Val Arg  Arg Leu Asn Ala Val  Leu Leu Pro Asn Val  His Thr Leu 
    2195                 2200                 2205             


Phe Asp  Met Ser Ala Glu Asp  Phe Asp Ala Ile Ile  Ala Ala His 
    2210                 2215                 2220             


Phe Lys  Pro Gly Asp Thr Val  Leu Glu Thr Asp Ile  Ala Ser Phe 
    2225                 2230                 2235             


Asp Lys  Ser Gln Asp Asp Ser  Leu Ala Leu Thr Ala  Leu Met Leu 
    2240                 2245                 2250             


Leu Glu  Asp Leu Gly Val Asp  His Ser Leu Leu Asp  Leu Ile Glu 
    2255                 2260                 2265             


Ala Ala  Phe Gly Glu Ile Ser  Ser Cys His Leu Pro  Thr Gly Thr 
    2270                 2275                 2280             


Arg Phe  Lys Phe Gly Ala Met  Met Lys Ser Gly Met  Phe Leu Thr 
    2285                 2290                 2295             


Leu Phe  Val Asn Thr Leu Leu  Asn Ile Thr Ile Ala  Ser Arg Val 
    2300                 2305                 2310             


Leu Glu  Asp Arg Leu Thr Lys  Ser Ala Cys Ala Ala  Phe Ile Gly 
    2315                 2320                 2325             


Asp Asp  Asn Ile Ile His Gly  Val Val Ser Asp Glu  Leu Met Ala 
    2330                 2335                 2340             


Ala Arg  Cys Ala Thr Trp Met  Asn Met Glu Val Lys  Ile Ile Asp 
    2345                 2350                 2355             


Ala Val  Val Ser Leu Lys Ala  Pro Tyr Phe Cys Gly  Gly Phe Ile 
    2360                 2365                 2370             


Leu His  Asp Thr Val Thr Gly  Thr Ala Cys Arg Val  Ala Asp Pro 
    2375                 2380                 2385             


Leu Lys  Arg Leu Phe Lys Leu  Gly Lys Pro Leu Ala  Ala Gly Asp 
    2390                 2395                 2400             


Glu Gln  Asp Glu Asp Arg Arg  Arg Ala Leu Ala Asp  Glu Val Ile 
    2405                 2410                 2415             


Arg Trp  Gln Arg Thr Gly Leu  Ile Asp Glu Leu Glu  Lys Ala Val 
    2420                 2425                 2430             


Tyr Ser  Arg Tyr Glu Val Gln  Gly Ile Ser Val Val  Val Met Ser 
    2435                 2440                 2445             


Met Ala  Thr Phe Ala Ser Ser  Arg Ser Asn Phe Glu  Lys Leu Arg 
    2450                 2455                 2460             


Gly Pro  Val Ile Thr Leu Tyr  Gly Gly Pro Lys 
    2465                 2470                 


<210>  3
<211>  2474
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide sequence of CHIKV LR2006 OPY1 non-structural 
       polyprotein with mutation at position 1050 from glutamic acid to 
       valine.

<400>  3

Met Asp Pro Val Tyr Val Asp Ile Asp Ala Asp Ser Ala Phe Leu Lys 
1               5                   10                  15      


Ala Leu Gln Arg Ala Tyr Pro Met Phe Glu Val Glu Pro Arg Gln Val 
            20                  25                  30          


Thr Pro Asn Asp His Ala Asn Ala Arg Ala Phe Ser His Leu Ala Ile 
        35                  40                  45              


Lys Leu Ile Glu Gln Glu Ile Asp Pro Asp Ser Thr Ile Leu Asp Ile 
    50                  55                  60                  


Gly Ser Ala Pro Ala Arg Arg Met Met Ser Asp Arg Lys Tyr His Cys 
65                  70                  75                  80  


Val Cys Pro Met Arg Ser Ala Glu Asp Pro Glu Arg Leu Ala Asn Tyr 
                85                  90                  95      


Ala Arg Lys Leu Ala Ser Ala Ala Gly Lys Val Leu Asp Arg Asn Ile 
            100                 105                 110         


Ser Gly Lys Ile Gly Asp Leu Gln Ala Val Met Ala Val Pro Asp Thr 
        115                 120                 125             


Glu Thr Pro Thr Phe Cys Leu His Thr Asp Val Ser Cys Arg Gln Arg 
    130                 135                 140                 


Ala Asp Val Ala Ile Tyr Gln Asp Val Tyr Ala Val His Ala Pro Thr 
145                 150                 155                 160 


Ser Leu Tyr His Gln Ala Ile Lys Gly Val Arg Val Ala Tyr Trp Val 
                165                 170                 175     


Gly Phe Asp Thr Thr Pro Phe Met Tyr Asn Ala Met Ala Gly Ala Tyr 
            180                 185                 190         


Pro Ser Tyr Ser Thr Asn Trp Ala Asp Glu Gln Val Leu Lys Ala Lys 
        195                 200                 205             


Asn Ile Gly Leu Cys Ser Thr Asp Leu Thr Glu Gly Arg Arg Gly Lys 
    210                 215                 220                 


Leu Ser Ile Met Arg Gly Lys Lys Leu Lys Pro Cys Asp Arg Val Leu 
225                 230                 235                 240 


Phe Ser Val Gly Ser Thr Leu Tyr Pro Glu Ser Arg Lys Leu Leu Lys 
                245                 250                 255     


Ser Trp His Leu Pro Ser Val Phe His Leu Lys Gly Lys Leu Ser Phe 
            260                 265                 270         


Thr Cys Arg Cys Asp Thr Val Val Ser Cys Glu Gly Tyr Val Val Lys 
        275                 280                 285             


Arg Ile Thr Met Ser Pro Gly Leu Tyr Gly Lys Thr Thr Gly Tyr Ala 
    290                 295                 300                 


Val Thr His His Ala Asp Gly Phe Leu Met Cys Lys Thr Thr Asp Thr 
305                 310                 315                 320 


Val Asp Gly Glu Arg Met Ser Phe Ser Val Cys Thr Tyr Val Pro Ala 
                325                 330                 335     


Thr Ile Cys Asp Gln Met Thr Gly Ile Leu Ala Thr Glu Val Thr Pro 
            340                 345                 350         


Glu Asp Ala Gln Lys Leu Leu Val Gly Leu Asn Gln Arg Ile Val Val 
        355                 360                 365             


Asn Gly Arg Thr Gln Arg Asn Thr Asn Thr Met Lys Asn Tyr Leu Leu 
    370                 375                 380                 


Pro Val Val Ala Gln Ala Phe Ser Lys Trp Ala Lys Glu Cys Arg Lys 
385                 390                 395                 400 


Asp Met Glu Asp Glu Lys Leu Leu Gly Val Arg Glu Arg Thr Leu Thr 
                405                 410                 415     


Cys Cys Cys Leu Trp Ala Phe Lys Lys Gln Lys Thr His Thr Val Tyr 
            420                 425                 430         


Lys Arg Pro Asp Thr Gln Ser Ile Gln Lys Val Gln Ala Glu Phe Asp 
        435                 440                 445             


Ser Phe Val Val Pro Ser Leu Trp Ser Ser Gly Leu Ser Ile Pro Leu 
    450                 455                 460                 


Arg Thr Arg Ile Lys Trp Leu Leu Ser Lys Val Pro Lys Thr Asp Leu 
465                 470                 475                 480 


Ile Pro Tyr Ser Gly Asp Ala Arg Glu Ala Arg Asp Ala Glu Lys Glu 
                485                 490                 495     


Ala Glu Glu Glu Arg Glu Ala Glu Leu Thr Arg Glu Ala Leu Pro Pro 
            500                 505                 510         


Leu Gln Ala Ala Gln Glu Asp Val Gln Val Glu Ile Asp Val Glu Gln 
        515                 520                 525             


Leu Glu Asp Arg Ala Gly Ala Gly Ile Ile Glu Thr Pro Arg Gly Ala 
    530                 535                 540                 


Ile Lys Val Thr Ala Gln Pro Thr Asp His Val Val Gly Glu Tyr Leu 
545                 550                 555                 560 


Val Leu Ser Pro Gln Thr Val Leu Arg Ser Gln Lys Leu Ser Leu Ile 
                565                 570                 575     


His Ala Leu Ala Glu Gln Val Lys Thr Cys Thr His Asn Gly Arg Ala 
            580                 585                 590         


Gly Arg Tyr Ala Val Glu Ala Tyr Asp Gly Arg Val Leu Val Pro Ser 
        595                 600                 605             


Gly Tyr Ala Ile Ser Pro Glu Asp Phe Gln Ser Leu Ser Glu Ser Ala 
    610                 615                 620                 


Thr Met Val Tyr Asn Glu Arg Glu Phe Val Asn Arg Lys Leu His His 
625                 630                 635                 640 


Ile Ala Met His Gly Pro Ala Leu Asn Thr Asp Glu Glu Ser Tyr Glu 
                645                 650                 655     


Leu Val Arg Ala Glu Arg Thr Glu His Glu Tyr Val Tyr Asp Val Asp 
            660                 665                 670         


Gln Arg Arg Cys Cys Lys Lys Glu Glu Ala Ala Gly Leu Val Leu Val 
        675                 680                 685             


Gly Asp Leu Thr Asn Pro Pro Tyr His Glu Phe Ala Tyr Glu Gly Leu 
    690                 695                 700                 


Lys Ile Arg Pro Ala Cys Pro Tyr Lys Ile Ala Val Ile Gly Val Phe 
705                 710                 715                 720 


Gly Val Pro Gly Ser Gly Lys Ser Ala Ile Ile Lys Asn Leu Val Thr 
                725                 730                 735     


Arg Gln Asp Leu Val Thr Ser Gly Lys Lys Glu Asn Cys Gln Glu Ile 
            740                 745                 750         


Thr Thr Asp Val Met Arg Gln Arg Gly Leu Glu Ile Ser Ala Arg Thr 
        755                 760                 765             


Val Asp Ser Leu Leu Leu Asn Gly Cys Asn Arg Pro Val Asp Val Leu 
    770                 775                 780                 


Tyr Val Asp Glu Ala Phe Ala Cys His Ser Gly Thr Leu Leu Ala Leu 
785                 790                 795                 800 


Ile Ala Leu Val Arg Pro Arg Gln Lys Val Val Leu Cys Gly Asp Pro 
                805                 810                 815     


Lys Gln Cys Gly Phe Phe Asn Met Met Gln Met Lys Val Asn Tyr Asn 
            820                 825                 830         


His Asn Ile Cys Thr Gln Val Tyr His Lys Ser Ile Ser Arg Arg Cys 
        835                 840                 845             


Thr Leu Pro Val Thr Ala Ile Val Ser Ser Leu His Tyr Glu Gly Lys 
    850                 855                 860                 


Met Arg Thr Thr Asn Glu Tyr Asn Lys Pro Ile Val Val Asp Thr Thr 
865                 870                 875                 880 


Gly Ser Thr Lys Pro Asp Pro Gly Asp Leu Val Leu Thr Cys Phe Arg 
                885                 890                 895     


Gly Trp Val Lys Gln Leu Gln Ile Asp Tyr Arg Gly Tyr Glu Val Met 
            900                 905                 910         


Thr Ala Ala Ala Ser Gln Gly Leu Thr Arg Lys Gly Val Tyr Ala Val 
        915                 920                 925             


Arg Gln Lys Val Asn Glu Asn Pro Leu Tyr Ala Ser Thr Ser Glu His 
    930                 935                 940                 


Val Asn Val Leu Leu Thr Arg Thr Glu Gly Lys Leu Val Trp Lys Thr 
945                 950                 955                 960 


Leu Ser Gly Asp Pro Trp Ile Lys Thr Leu Gln Asn Pro Pro Lys Gly 
                965                 970                 975     


Asn Phe Lys Ala Thr Ile Lys Glu Trp Glu Val Glu His Ala Ser Ile 
            980                 985                 990         


Met Ala Gly Ile Cys Ser His Gln  Met Thr Phe Asp Thr  Phe Gln Asn 
        995                 1000                 1005             


Lys Ala  Asn Val Cys Trp Ala  Lys Ser Leu Val Pro  Ile Leu Glu 
    1010                 1015                 1020             


Thr Ala  Gly Ile Lys Leu Asn  Asp Arg Gln Trp Ser  Gln Ile Ile 
    1025                 1030                 1035             


Gln Ala  Phe Lys Glu Asp Lys  Ala Tyr Ser Pro Val  Val Ala Leu 
    1040                 1045                 1050             


Asn Glu  Ile Cys Thr Arg Met  Tyr Gly Val Asp Leu  Asp Ser Gly 
    1055                 1060                 1065             


Leu Phe  Ser Lys Pro Leu Val  Ser Val Tyr Tyr Ala  Asp Asn His 
    1070                 1075                 1080             


Trp Asp  Asn Arg Pro Gly Gly  Lys Met Phe Gly Phe  Asn Pro Glu 
    1085                 1090                 1095             


Ala Ala  Ser Ile Leu Glu Arg  Lys Tyr Pro Phe Thr  Lys Gly Lys 
    1100                 1105                 1110             


Trp Asn  Ile Asn Lys Gln Ile  Cys Val Thr Thr Arg  Arg Ile Glu 
    1115                 1120                 1125             


Asp Phe  Asn Pro Thr Thr Asn  Ile Ile Pro Ala Asn  Arg Arg Leu 
    1130                 1135                 1140             


Pro His  Ser Leu Val Ala Glu  His Arg Pro Val Lys  Gly Glu Arg 
    1145                 1150                 1155             


Met Glu  Trp Leu Val Asn Lys  Ile Asn Gly His His  Val Leu Leu 
    1160                 1165                 1170             


Val Ser  Gly Tyr Asn Leu Ala  Leu Pro Thr Lys Arg  Val Thr Trp 
    1175                 1180                 1185             


Val Ala  Pro Leu Gly Val Arg  Gly Ala Asp Tyr Thr  Tyr Asn Leu 
    1190                 1195                 1200             


Glu Leu  Gly Leu Pro Ala Thr  Leu Gly Arg Tyr Asp  Leu Val Val 
    1205                 1210                 1215             


Ile Asn  Ile His Thr Pro Phe  Arg Ile His His Tyr  Gln Gln Cys 
    1220                 1225                 1230             


Val Asp  His Ala Met Lys Leu  Gln Met Leu Gly Gly  Asp Ser Leu 
    1235                 1240                 1245             


Arg Leu  Leu Lys Pro Gly Gly  Ser Leu Leu Ile Arg  Ala Tyr Gly 
    1250                 1255                 1260             


Tyr Ala  Asp Arg Thr Ser Glu  Arg Val Ile Cys Val  Leu Gly Arg 
    1265                 1270                 1275             


Lys Phe  Arg Ser Ser Arg Ala  Leu Lys Pro Pro Cys  Val Thr Ser 
    1280                 1285                 1290             


Asn Thr  Glu Met Phe Phe Leu  Phe Ser Asn Phe Asp  Asn Gly Arg 
    1295                 1300                 1305             


Arg Asn  Phe Thr Thr His Val  Met Asn Asn Gln Leu  Asn Ala Ala 
    1310                 1315                 1320             


Phe Val  Gly Gln Val Thr Arg  Ala Gly Cys Ala Pro  Ser Tyr Arg 
    1325                 1330                 1335             


Val Lys  Arg Met Asp Ile Ala  Lys Asn Asp Glu Glu  Cys Val Val 
    1340                 1345                 1350             


Asn Ala  Ala Asn Pro Arg Gly  Leu Pro Gly Gly Gly  Val Cys Lys 
    1355                 1360                 1365             


Ala Val  Tyr Lys Lys Trp Pro  Glu Ser Phe Lys Asn  Ser Ala Thr 
    1370                 1375                 1380             


Pro Val  Gly Thr Ala Lys Thr  Val Met Cys Gly Thr  Tyr Pro Val 
    1385                 1390                 1395             


Ile His  Ala Val Gly Pro Asn  Phe Ser Asn Tyr Ser  Glu Ser Glu 
    1400                 1405                 1410             


Gly Asp  Arg Glu Leu Ala Ala  Ala Tyr Arg Glu Val  Ala Lys Glu 
    1415                 1420                 1425             


Val Thr  Arg Leu Gly Val Asn  Ser Val Ala Ile Pro  Leu Leu Ser 
    1430                 1435                 1440             


Thr Gly  Val Tyr Ser Gly Gly  Lys Asp Arg Leu Thr  Gln Ser Leu 
    1445                 1450                 1455             


Asn His  Leu Phe Thr Ala Met  Asp Ser Thr Asp Ala  Asp Val Val 
    1460                 1465                 1470             


Ile Tyr  Cys Arg Asp Lys Glu  Trp Glu Lys Lys Ile  Ser Glu Ala 
    1475                 1480                 1485             


Ile Gln  Met Arg Thr Gln Val  Glu Leu Leu Asp Glu  His Ile Ser 
    1490                 1495                 1500             


Ile Asp  Cys Asp Ile Val Arg  Val His Pro Asp Ser  Ser Leu Ala 
    1505                 1510                 1515             


Gly Arg  Lys Gly Tyr Ser Thr  Thr Glu Gly Ala Leu  Tyr Ser Tyr 
    1520                 1525                 1530             


Leu Glu  Gly Thr Arg Phe His  Gln Thr Ala Val Asp  Met Ala Glu 
    1535                 1540                 1545             


Ile His  Thr Met Trp Pro Lys  Gln Thr Glu Ala Asn  Glu Gln Val 
    1550                 1555                 1560             


Cys Leu  Tyr Ala Leu Gly Glu  Ser Ile Glu Ser Ile  Arg Gln Lys 
    1565                 1570                 1575             


Cys Pro  Val Asp Asp Ala Asp  Ala Ser Ser Pro Pro  Lys Thr Val 
    1580                 1585                 1590             


Pro Cys  Leu Cys Arg Tyr Ala  Met Thr Pro Glu Arg  Val Thr Arg 
    1595                 1600                 1605             


Leu Arg  Met Asn His Val Thr  Ser Ile Ile Val Cys  Ser Ser Phe 
    1610                 1615                 1620             


Pro Leu  Pro Lys Tyr Lys Ile  Glu Gly Val Gln Lys  Val Lys Cys 
    1625                 1630                 1635             


Ser Lys  Val Met Leu Phe Asp  His Asn Val Pro Ser  Arg Val Ser 
    1640                 1645                 1650             


Pro Arg  Glu Tyr Arg Ser Ser  Gln Glu Ser Ala Gln  Glu Ala Ser 
    1655                 1660                 1665             


Thr Ile  Thr Ser Leu Thr His  Ser Gln Phe Asp Leu  Ser Val Asp 
    1670                 1675                 1680             


Gly Glu  Ile Leu Pro Val Pro  Ser Asp Leu Asp Ala  Asp Ala Pro 
    1685                 1690                 1695             


Ala Leu  Glu Pro Ala Leu Asp  Asp Gly Ala Thr His  Thr Leu Pro 
    1700                 1705                 1710             


Ser Thr  Thr Gly Asn Leu Ala  Ala Val Ser Asp Trp  Val Met Ser 
    1715                 1720                 1725             


Thr Val  Pro Val Ala Pro Pro  Arg Arg Arg Arg Gly  Arg Asn Leu 
    1730                 1735                 1740             


Thr Val  Thr Cys Asp Glu Arg  Glu Gly Asn Ile Thr  Pro Met Ala 
    1745                 1750                 1755             


Ser Val  Arg Phe Phe Arg Ala  Glu Leu Cys Pro Val  Val Gln Glu 
    1760                 1765                 1770             


Thr Ala  Glu Thr Arg Asp Thr  Ala Met Ser Leu Gln  Ala Pro Pro 
    1775                 1780                 1785             


Ser Thr  Ala Thr Glu Pro Asn  His Pro Pro Ile Ser  Phe Gly Ala 
    1790                 1795                 1800             


Ser Ser  Glu Thr Phe Pro Ile  Thr Phe Gly Asp Phe  Asn Glu Gly 
    1805                 1810                 1815             


Glu Ile  Glu Ser Leu Ser Ser  Glu Leu Leu Thr Phe  Gly Asp Phe 
    1820                 1825                 1830             


Leu Pro  Gly Glu Val Asp Asp  Leu Thr Asp Ser Asp  Trp Ser Thr 
    1835                 1840                 1845             


Cys Ser  Asp Thr Asp Asp Glu  Leu Arg Leu Asp Arg  Ala Gly Gly 
    1850                 1855                 1860             


Tyr Ile  Phe Ser Ser Asp Thr  Gly Pro Gly His Leu  Gln Gln Lys 
    1865                 1870                 1875             


Ser Val  Arg Gln Ser Val Leu  Pro Val Asn Thr Leu  Glu Glu Val 
    1880                 1885                 1890             


His Glu  Glu Lys Cys Tyr Pro  Pro Lys Leu Asp Glu  Ala Lys Glu 
    1895                 1900                 1905             


Gln Leu  Leu Leu Lys Lys Leu  Gln Glu Ser Ala Ser  Met Ala Asn 
    1910                 1915                 1920             


Arg Ser  Arg Tyr Gln Ser Arg  Lys Val Glu Asn Met  Lys Ala Ala 
    1925                 1930                 1935             


Ile Ile  Gln Arg Leu Lys Arg  Gly Cys Arg Leu Tyr  Leu Met Ser 
    1940                 1945                 1950             


Glu Thr  Pro Lys Val Pro Thr  Tyr Arg Thr Thr Tyr  Pro Ala Pro 
    1955                 1960                 1965             


Val Tyr  Ser Pro Pro Ile Asn  Val Arg Leu Ser Asn  Pro Glu Ser 
    1970                 1975                 1980             


Ala Val  Ala Ala Cys Asn Glu  Phe Leu Ala Arg Asn  Tyr Pro Thr 
    1985                 1990                 1995             


Val Ser  Ser Tyr Gln Ile Thr  Asp Glu Tyr Asp Ala  Tyr Leu Asp 
    2000                 2005                 2010             


Met Val  Asp Gly Ser Glu Ser  Cys Leu Asp Arg Ala  Thr Phe Asn 
    2015                 2020                 2025             


Pro Ser  Lys Leu Arg Ser Tyr  Pro Lys Gln His Ala  Tyr His Ala 
    2030                 2035                 2040             


Pro Ser  Ile Arg Ser Ala Val  Pro Ser Pro Phe Gln  Asn Thr Leu 
    2045                 2050                 2055             


Gln Asn  Val Leu Ala Ala Ala  Thr Lys Arg Asn Cys  Asn Val Thr 
    2060                 2065                 2070             


Gln Met  Arg Glu Leu Pro Thr  Leu Asp Ser Ala Val  Phe Asn Val 
    2075                 2080                 2085             


Glu Cys  Phe Lys Lys Phe Ala  Cys Asn Gln Glu Tyr  Trp Glu Glu 
    2090                 2095                 2100             


Phe Ala  Ala Ser Pro Ile Arg  Ile Thr Thr Glu Asn  Leu Ala Thr 
    2105                 2110                 2115             


Tyr Val  Thr Lys Leu Lys Gly  Pro Lys Ala Ala Ala  Leu Phe Ala 
    2120                 2125                 2130             


Lys Thr  His Asn Leu Leu Pro  Leu Gln Glu Val Pro  Met Asp Arg 
    2135                 2140                 2145             


Phe Thr  Val Asp Met Lys Arg  Asp Val Lys Val Thr  Pro Gly Thr 
    2150                 2155                 2160             


Lys His  Thr Glu Glu Arg Pro  Lys Val Gln Val Ile  Gln Ala Ala 
    2165                 2170                 2175             


Glu Pro  Leu Ala Thr Ala Tyr  Leu Cys Gly Ile His  Arg Glu Leu 
    2180                 2185                 2190             


Val Arg  Arg Leu Asn Ala Val  Leu Leu Pro Asn Val  His Thr Leu 
    2195                 2200                 2205             


Phe Asp  Met Ser Ala Glu Asp  Phe Asp Ala Ile Ile  Ala Ala His 
    2210                 2215                 2220             


Phe Lys  Pro Gly Asp Thr Val  Leu Glu Thr Asp Ile  Ala Ser Phe 
    2225                 2230                 2235             


Asp Lys  Ser Gln Asp Asp Ser  Leu Ala Leu Thr Ala  Leu Met Leu 
    2240                 2245                 2250             


Leu Glu  Asp Leu Gly Val Asp  His Ser Leu Leu Asp  Leu Ile Glu 
    2255                 2260                 2265             


Ala Ala  Phe Gly Glu Ile Ser  Ser Cys His Leu Pro  Thr Gly Thr 
    2270                 2275                 2280             


Arg Phe  Lys Phe Gly Ala Met  Met Lys Ser Gly Met  Phe Leu Thr 
    2285                 2290                 2295             


Leu Phe  Val Asn Thr Leu Leu  Asn Ile Thr Ile Ala  Ser Arg Val 
    2300                 2305                 2310             


Leu Glu  Asp Arg Leu Thr Lys  Ser Ala Cys Ala Ala  Phe Ile Gly 
    2315                 2320                 2325             


Asp Asp  Asn Ile Ile His Gly  Val Val Ser Asp Glu  Leu Met Ala 
    2330                 2335                 2340             


Ala Arg  Cys Ala Thr Trp Met  Asn Met Glu Val Lys  Ile Ile Asp 
    2345                 2350                 2355             


Ala Val  Val Ser Leu Lys Ala  Pro Tyr Phe Cys Gly  Gly Phe Ile 
    2360                 2365                 2370             


Leu His  Asp Thr Val Thr Gly  Thr Ala Cys Arg Val  Ala Asp Pro 
    2375                 2380                 2385             


Leu Lys  Arg Leu Phe Lys Leu  Gly Lys Pro Leu Ala  Ala Gly Asp 
    2390                 2395                 2400             


Glu Gln  Asp Glu Asp Arg Arg  Arg Ala Leu Ala Asp  Glu Val Ile 
    2405                 2410                 2415             


Arg Trp  Gln Arg Thr Gly Leu  Ile Asp Glu Leu Glu  Lys Ala Val 
    2420                 2425                 2430             


Tyr Ser  Arg Tyr Glu Val Gln  Gly Ile Ser Val Val  Val Met Ser 
    2435                 2440                 2445             


Met Ala  Thr Phe Ala Ser Ser  Arg Ser Asn Phe Glu  Lys Leu Arg 
    2450                 2455                 2460             


Gly Pro  Val Ile Thr Leu Tyr  Gly Gly Pro Lys 
    2465                 2470                 


<210>  4
<211>  2474
<212>  PRT
<213>  artificial sequence

<220>
<223>  Polypeptide sequence of CHIKV LR2006 OPY1 non-structural 
       polyprotein with mutations at 532 from arginine to histidine and 
       at 1050 from glutamic acid to valine.

<400>  4

Met Asp Pro Val Tyr Val Asp Ile Asp Ala Asp Ser Ala Phe Leu Lys 
1               5                   10                  15      


Ala Leu Gln Arg Ala Tyr Pro Met Phe Glu Val Glu Pro Arg Gln Val 
            20                  25                  30          


Thr Pro Asn Asp His Ala Asn Ala Arg Ala Phe Ser His Leu Ala Ile 
        35                  40                  45              


Lys Leu Ile Glu Gln Glu Ile Asp Pro Asp Ser Thr Ile Leu Asp Ile 
    50                  55                  60                  


Gly Ser Ala Pro Ala Arg Arg Met Met Ser Asp Arg Lys Tyr His Cys 
65                  70                  75                  80  


Val Cys Pro Met Arg Ser Ala Glu Asp Pro Glu Arg Leu Ala Asn Tyr 
                85                  90                  95      


Ala Arg Lys Leu Ala Ser Ala Ala Gly Lys Val Leu Asp Arg Asn Ile 
            100                 105                 110         


Ser Gly Lys Ile Gly Asp Leu Gln Ala Val Met Ala Val Pro Asp Thr 
        115                 120                 125             


Glu Thr Pro Thr Phe Cys Leu His Thr Asp Val Ser Cys Arg Gln Arg 
    130                 135                 140                 


Ala Asp Val Ala Ile Tyr Gln Asp Val Tyr Ala Val His Ala Pro Thr 
145                 150                 155                 160 


Ser Leu Tyr His Gln Ala Ile Lys Gly Val Arg Val Ala Tyr Trp Val 
                165                 170                 175     


Gly Phe Asp Thr Thr Pro Phe Met Tyr Asn Ala Met Ala Gly Ala Tyr 
            180                 185                 190         


Pro Ser Tyr Ser Thr Asn Trp Ala Asp Glu Gln Val Leu Lys Ala Lys 
        195                 200                 205             


Asn Ile Gly Leu Cys Ser Thr Asp Leu Thr Glu Gly Arg Arg Gly Lys 
    210                 215                 220                 


Leu Ser Ile Met Arg Gly Lys Lys Leu Lys Pro Cys Asp Arg Val Leu 
225                 230                 235                 240 


Phe Ser Val Gly Ser Thr Leu Tyr Pro Glu Ser Arg Lys Leu Leu Lys 
                245                 250                 255     


Ser Trp His Leu Pro Ser Val Phe His Leu Lys Gly Lys Leu Ser Phe 
            260                 265                 270         


Thr Cys Arg Cys Asp Thr Val Val Ser Cys Glu Gly Tyr Val Val Lys 
        275                 280                 285             


Arg Ile Thr Met Ser Pro Gly Leu Tyr Gly Lys Thr Thr Gly Tyr Ala 
    290                 295                 300                 


Val Thr His His Ala Asp Gly Phe Leu Met Cys Lys Thr Thr Asp Thr 
305                 310                 315                 320 


Val Asp Gly Glu Arg Met Ser Phe Ser Val Cys Thr Tyr Val Pro Ala 
                325                 330                 335     


Thr Ile Cys Asp Gln Met Thr Gly Ile Leu Ala Thr Glu Val Thr Pro 
            340                 345                 350         


Glu Asp Ala Gln Lys Leu Leu Val Gly Leu Asn Gln Arg Ile Val Val 
        355                 360                 365             


Asn Gly Arg Thr Gln Arg Asn Thr Asn Thr Met Lys Asn Tyr Leu Leu 
    370                 375                 380                 


Pro Val Val Ala Gln Ala Phe Ser Lys Trp Ala Lys Glu Cys Arg Lys 
385                 390                 395                 400 


Asp Met Glu Asp Glu Lys Leu Leu Gly Val Arg Glu Arg Thr Leu Thr 
                405                 410                 415     


Cys Cys Cys Leu Trp Ala Phe Lys Lys Gln Lys Thr His Thr Val Tyr 
            420                 425                 430         


Lys Arg Pro Asp Thr Gln Ser Ile Gln Lys Val Gln Ala Glu Phe Asp 
        435                 440                 445             


Ser Phe Val Val Pro Ser Leu Trp Ser Ser Gly Leu Ser Ile Pro Leu 
    450                 455                 460                 


Arg Thr Arg Ile Lys Trp Leu Leu Ser Lys Val Pro Lys Thr Asp Leu 
465                 470                 475                 480 


Ile Pro Tyr Ser Gly Asp Ala Arg Glu Ala Arg Asp Ala Glu Lys Glu 
                485                 490                 495     


Ala Glu Glu Glu Arg Glu Ala Glu Leu Thr Arg Glu Ala Leu Pro Pro 
            500                 505                 510         


Leu Gln Ala Ala Gln Glu Asp Val Gln Val Glu Ile Asp Val Glu Gln 
        515                 520                 525             


Leu Glu Asp His Ala Gly Ala Gly Ile Ile Glu Thr Pro Arg Gly Ala 
    530                 535                 540                 


Ile Lys Val Thr Ala Gln Pro Thr Asp His Val Val Gly Glu Tyr Leu 
545                 550                 555                 560 


Val Leu Ser Pro Gln Thr Val Leu Arg Ser Gln Lys Leu Ser Leu Ile 
                565                 570                 575     


His Ala Leu Ala Glu Gln Val Lys Thr Cys Thr His Asn Gly Arg Ala 
            580                 585                 590         


Gly Arg Tyr Ala Val Glu Ala Tyr Asp Gly Arg Val Leu Val Pro Ser 
        595                 600                 605             


Gly Tyr Ala Ile Ser Pro Glu Asp Phe Gln Ser Leu Ser Glu Ser Ala 
    610                 615                 620                 


Thr Met Val Tyr Asn Glu Arg Glu Phe Val Asn Arg Lys Leu His His 
625                 630                 635                 640 


Ile Ala Met His Gly Pro Ala Leu Asn Thr Asp Glu Glu Ser Tyr Glu 
                645                 650                 655     


Leu Val Arg Ala Glu Arg Thr Glu His Glu Tyr Val Tyr Asp Val Asp 
            660                 665                 670         


Gln Arg Arg Cys Cys Lys Lys Glu Glu Ala Ala Gly Leu Val Leu Val 
        675                 680                 685             


Gly Asp Leu Thr Asn Pro Pro Tyr His Glu Phe Ala Tyr Glu Gly Leu 
    690                 695                 700                 


Lys Ile Arg Pro Ala Cys Pro Tyr Lys Ile Ala Val Ile Gly Val Phe 
705                 710                 715                 720 


Gly Val Pro Gly Ser Gly Lys Ser Ala Ile Ile Lys Asn Leu Val Thr 
                725                 730                 735     


Arg Gln Asp Leu Val Thr Ser Gly Lys Lys Glu Asn Cys Gln Glu Ile 
            740                 745                 750         


Thr Thr Asp Val Met Arg Gln Arg Gly Leu Glu Ile Ser Ala Arg Thr 
        755                 760                 765             


Val Asp Ser Leu Leu Leu Asn Gly Cys Asn Arg Pro Val Asp Val Leu 
    770                 775                 780                 


Tyr Val Asp Glu Ala Phe Ala Cys His Ser Gly Thr Leu Leu Ala Leu 
785                 790                 795                 800 


Ile Ala Leu Val Arg Pro Arg Gln Lys Val Val Leu Cys Gly Asp Pro 
                805                 810                 815     


Lys Gln Cys Gly Phe Phe Asn Met Met Gln Met Lys Val Asn Tyr Asn 
            820                 825                 830         


His Asn Ile Cys Thr Gln Val Tyr His Lys Ser Ile Ser Arg Arg Cys 
        835                 840                 845             


Thr Leu Pro Val Thr Ala Ile Val Ser Ser Leu His Tyr Glu Gly Lys 
    850                 855                 860                 


Met Arg Thr Thr Asn Glu Tyr Asn Lys Pro Ile Val Val Asp Thr Thr 
865                 870                 875                 880 


Gly Ser Thr Lys Pro Asp Pro Gly Asp Leu Val Leu Thr Cys Phe Arg 
                885                 890                 895     


Gly Trp Val Lys Gln Leu Gln Ile Asp Tyr Arg Gly Tyr Glu Val Met 
            900                 905                 910         


Thr Ala Ala Ala Ser Gln Gly Leu Thr Arg Lys Gly Val Tyr Ala Val 
        915                 920                 925             


Arg Gln Lys Val Asn Glu Asn Pro Leu Tyr Ala Ser Thr Ser Glu His 
    930                 935                 940                 


Val Asn Val Leu Leu Thr Arg Thr Glu Gly Lys Leu Val Trp Lys Thr 
945                 950                 955                 960 


Leu Ser Gly Asp Pro Trp Ile Lys Thr Leu Gln Asn Pro Pro Lys Gly 
                965                 970                 975     


Asn Phe Lys Ala Thr Ile Lys Glu Trp Glu Val Glu His Ala Ser Ile 
            980                 985                 990         


Met Ala Gly Ile Cys Ser His Gln  Met Thr Phe Asp Thr  Phe Gln Asn 
        995                 1000                 1005             


Lys Ala  Asn Val Cys Trp Ala  Lys Ser Leu Val Pro  Ile Leu Glu 
    1010                 1015                 1020             


Thr Ala  Gly Ile Lys Leu Asn  Asp Arg Gln Trp Ser  Gln Ile Ile 
    1025                 1030                 1035             


Gln Ala  Phe Lys Glu Asp Lys  Ala Tyr Ser Pro Val  Val Ala Leu 
    1040                 1045                 1050             


Asn Glu  Ile Cys Thr Arg Met  Tyr Gly Val Asp Leu  Asp Ser Gly 
    1055                 1060                 1065             


Leu Phe  Ser Lys Pro Leu Val  Ser Val Tyr Tyr Ala  Asp Asn His 
    1070                 1075                 1080             


Trp Asp  Asn Arg Pro Gly Gly  Lys Met Phe Gly Phe  Asn Pro Glu 
    1085                 1090                 1095             


Ala Ala  Ser Ile Leu Glu Arg  Lys Tyr Pro Phe Thr  Lys Gly Lys 
    1100                 1105                 1110             


Trp Asn  Ile Asn Lys Gln Ile  Cys Val Thr Thr Arg  Arg Ile Glu 
    1115                 1120                 1125             


Asp Phe  Asn Pro Thr Thr Asn  Ile Ile Pro Ala Asn  Arg Arg Leu 
    1130                 1135                 1140             


Pro His  Ser Leu Val Ala Glu  His Arg Pro Val Lys  Gly Glu Arg 
    1145                 1150                 1155             


Met Glu  Trp Leu Val Asn Lys  Ile Asn Gly His His  Val Leu Leu 
    1160                 1165                 1170             


Val Ser  Gly Tyr Asn Leu Ala  Leu Pro Thr Lys Arg  Val Thr Trp 
    1175                 1180                 1185             


Val Ala  Pro Leu Gly Val Arg  Gly Ala Asp Tyr Thr  Tyr Asn Leu 
    1190                 1195                 1200             


Glu Leu  Gly Leu Pro Ala Thr  Leu Gly Arg Tyr Asp  Leu Val Val 
    1205                 1210                 1215             


Ile Asn  Ile His Thr Pro Phe  Arg Ile His His Tyr  Gln Gln Cys 
    1220                 1225                 1230             


Val Asp  His Ala Met Lys Leu  Gln Met Leu Gly Gly  Asp Ser Leu 
    1235                 1240                 1245             


Arg Leu  Leu Lys Pro Gly Gly  Ser Leu Leu Ile Arg  Ala Tyr Gly 
    1250                 1255                 1260             


Tyr Ala  Asp Arg Thr Ser Glu  Arg Val Ile Cys Val  Leu Gly Arg 
    1265                 1270                 1275             


Lys Phe  Arg Ser Ser Arg Ala  Leu Lys Pro Pro Cys  Val Thr Ser 
    1280                 1285                 1290             


Asn Thr  Glu Met Phe Phe Leu  Phe Ser Asn Phe Asp  Asn Gly Arg 
    1295                 1300                 1305             


Arg Asn  Phe Thr Thr His Val  Met Asn Asn Gln Leu  Asn Ala Ala 
    1310                 1315                 1320             


Phe Val  Gly Gln Val Thr Arg  Ala Gly Cys Ala Pro  Ser Tyr Arg 
    1325                 1330                 1335             


Val Lys  Arg Met Asp Ile Ala  Lys Asn Asp Glu Glu  Cys Val Val 
    1340                 1345                 1350             


Asn Ala  Ala Asn Pro Arg Gly  Leu Pro Gly Gly Gly  Val Cys Lys 
    1355                 1360                 1365             


Ala Val  Tyr Lys Lys Trp Pro  Glu Ser Phe Lys Asn  Ser Ala Thr 
    1370                 1375                 1380             


Pro Val  Gly Thr Ala Lys Thr  Val Met Cys Gly Thr  Tyr Pro Val 
    1385                 1390                 1395             


Ile His  Ala Val Gly Pro Asn  Phe Ser Asn Tyr Ser  Glu Ser Glu 
    1400                 1405                 1410             


Gly Asp  Arg Glu Leu Ala Ala  Ala Tyr Arg Glu Val  Ala Lys Glu 
    1415                 1420                 1425             


Val Thr  Arg Leu Gly Val Asn  Ser Val Ala Ile Pro  Leu Leu Ser 
    1430                 1435                 1440             


Thr Gly  Val Tyr Ser Gly Gly  Lys Asp Arg Leu Thr  Gln Ser Leu 
    1445                 1450                 1455             


Asn His  Leu Phe Thr Ala Met  Asp Ser Thr Asp Ala  Asp Val Val 
    1460                 1465                 1470             


Ile Tyr  Cys Arg Asp Lys Glu  Trp Glu Lys Lys Ile  Ser Glu Ala 
    1475                 1480                 1485             


Ile Gln  Met Arg Thr Gln Val  Glu Leu Leu Asp Glu  His Ile Ser 
    1490                 1495                 1500             


Ile Asp  Cys Asp Ile Val Arg  Val His Pro Asp Ser  Ser Leu Ala 
    1505                 1510                 1515             


Gly Arg  Lys Gly Tyr Ser Thr  Thr Glu Gly Ala Leu  Tyr Ser Tyr 
    1520                 1525                 1530             


Leu Glu  Gly Thr Arg Phe His  Gln Thr Ala Val Asp  Met Ala Glu 
    1535                 1540                 1545             


Ile His  Thr Met Trp Pro Lys  Gln Thr Glu Ala Asn  Glu Gln Val 
    1550                 1555                 1560             


Cys Leu  Tyr Ala Leu Gly Glu  Ser Ile Glu Ser Ile  Arg Gln Lys 
    1565                 1570                 1575             


Cys Pro  Val Asp Asp Ala Asp  Ala Ser Ser Pro Pro  Lys Thr Val 
    1580                 1585                 1590             


Pro Cys  Leu Cys Arg Tyr Ala  Met Thr Pro Glu Arg  Val Thr Arg 
    1595                 1600                 1605             


Leu Arg  Met Asn His Val Thr  Ser Ile Ile Val Cys  Ser Ser Phe 
    1610                 1615                 1620             


Pro Leu  Pro Lys Tyr Lys Ile  Glu Gly Val Gln Lys  Val Lys Cys 
    1625                 1630                 1635             


Ser Lys  Val Met Leu Phe Asp  His Asn Val Pro Ser  Arg Val Ser 
    1640                 1645                 1650             


Pro Arg  Glu Tyr Arg Ser Ser  Gln Glu Ser Ala Gln  Glu Ala Ser 
    1655                 1660                 1665             


Thr Ile  Thr Ser Leu Thr His  Ser Gln Phe Asp Leu  Ser Val Asp 
    1670                 1675                 1680             


Gly Glu  Ile Leu Pro Val Pro  Ser Asp Leu Asp Ala  Asp Ala Pro 
    1685                 1690                 1695             


Ala Leu  Glu Pro Ala Leu Asp  Asp Gly Ala Thr His  Thr Leu Pro 
    1700                 1705                 1710             


Ser Thr  Thr Gly Asn Leu Ala  Ala Val Ser Asp Trp  Val Met Ser 
    1715                 1720                 1725             


Thr Val  Pro Val Ala Pro Pro  Arg Arg Arg Arg Gly  Arg Asn Leu 
    1730                 1735                 1740             


Thr Val  Thr Cys Asp Glu Arg  Glu Gly Asn Ile Thr  Pro Met Ala 
    1745                 1750                 1755             


Ser Val  Arg Phe Phe Arg Ala  Glu Leu Cys Pro Val  Val Gln Glu 
    1760                 1765                 1770             


Thr Ala  Glu Thr Arg Asp Thr  Ala Met Ser Leu Gln  Ala Pro Pro 
    1775                 1780                 1785             


Ser Thr  Ala Thr Glu Pro Asn  His Pro Pro Ile Ser  Phe Gly Ala 
    1790                 1795                 1800             


Ser Ser  Glu Thr Phe Pro Ile  Thr Phe Gly Asp Phe  Asn Glu Gly 
    1805                 1810                 1815             


Glu Ile  Glu Ser Leu Ser Ser  Glu Leu Leu Thr Phe  Gly Asp Phe 
    1820                 1825                 1830             


Leu Pro  Gly Glu Val Asp Asp  Leu Thr Asp Ser Asp  Trp Ser Thr 
    1835                 1840                 1845             


Cys Ser  Asp Thr Asp Asp Glu  Leu Arg Leu Asp Arg  Ala Gly Gly 
    1850                 1855                 1860             


Tyr Ile  Phe Ser Ser Asp Thr  Gly Pro Gly His Leu  Gln Gln Lys 
    1865                 1870                 1875             


Ser Val  Arg Gln Ser Val Leu  Pro Val Asn Thr Leu  Glu Glu Val 
    1880                 1885                 1890             


His Glu  Glu Lys Cys Tyr Pro  Pro Lys Leu Asp Glu  Ala Lys Glu 
    1895                 1900                 1905             


Gln Leu  Leu Leu Lys Lys Leu  Gln Glu Ser Ala Ser  Met Ala Asn 
    1910                 1915                 1920             


Arg Ser  Arg Tyr Gln Ser Arg  Lys Val Glu Asn Met  Lys Ala Ala 
    1925                 1930                 1935             


Ile Ile  Gln Arg Leu Lys Arg  Gly Cys Arg Leu Tyr  Leu Met Ser 
    1940                 1945                 1950             


Glu Thr  Pro Lys Val Pro Thr  Tyr Arg Thr Thr Tyr  Pro Ala Pro 
    1955                 1960                 1965             


Val Tyr  Ser Pro Pro Ile Asn  Val Arg Leu Ser Asn  Pro Glu Ser 
    1970                 1975                 1980             


Ala Val  Ala Ala Cys Asn Glu  Phe Leu Ala Arg Asn  Tyr Pro Thr 
    1985                 1990                 1995             


Val Ser  Ser Tyr Gln Ile Thr  Asp Glu Tyr Asp Ala  Tyr Leu Asp 
    2000                 2005                 2010             


Met Val  Asp Gly Ser Glu Ser  Cys Leu Asp Arg Ala  Thr Phe Asn 
    2015                 2020                 2025             


Pro Ser  Lys Leu Arg Ser Tyr  Pro Lys Gln His Ala  Tyr His Ala 
    2030                 2035                 2040             


Pro Ser  Ile Arg Ser Ala Val  Pro Ser Pro Phe Gln  Asn Thr Leu 
    2045                 2050                 2055             


Gln Asn  Val Leu Ala Ala Ala  Thr Lys Arg Asn Cys  Asn Val Thr 
    2060                 2065                 2070             


Gln Met  Arg Glu Leu Pro Thr  Leu Asp Ser Ala Val  Phe Asn Val 
    2075                 2080                 2085             


Glu Cys  Phe Lys Lys Phe Ala  Cys Asn Gln Glu Tyr  Trp Glu Glu 
    2090                 2095                 2100             


Phe Ala  Ala Ser Pro Ile Arg  Ile Thr Thr Glu Asn  Leu Ala Thr 
    2105                 2110                 2115             


Tyr Val  Thr Lys Leu Lys Gly  Pro Lys Ala Ala Ala  Leu Phe Ala 
    2120                 2125                 2130             


Lys Thr  His Asn Leu Leu Pro  Leu Gln Glu Val Pro  Met Asp Arg 
    2135                 2140                 2145             


Phe Thr  Val Asp Met Lys Arg  Asp Val Lys Val Thr  Pro Gly Thr 
    2150                 2155                 2160             


Lys His  Thr Glu Glu Arg Pro  Lys Val Gln Val Ile  Gln Ala Ala 
    2165                 2170                 2175             


Glu Pro  Leu Ala Thr Ala Tyr  Leu Cys Gly Ile His  Arg Glu Leu 
    2180                 2185                 2190             


Val Arg  Arg Leu Asn Ala Val  Leu Leu Pro Asn Val  His Thr Leu 
    2195                 2200                 2205             


Phe Asp  Met Ser Ala Glu Asp  Phe Asp Ala Ile Ile  Ala Ala His 
    2210                 2215                 2220             


Phe Lys  Pro Gly Asp Thr Val  Leu Glu Thr Asp Ile  Ala Ser Phe 
    2225                 2230                 2235             


Asp Lys  Ser Gln Asp Asp Ser  Leu Ala Leu Thr Ala  Leu Met Leu 
    2240                 2245                 2250             


Leu Glu  Asp Leu Gly Val Asp  His Ser Leu Leu Asp  Leu Ile Glu 
    2255                 2260                 2265             


Ala Ala  Phe Gly Glu Ile Ser  Ser Cys His Leu Pro  Thr Gly Thr 
    2270                 2275                 2280             


Arg Phe  Lys Phe Gly Ala Met  Met Lys Ser Gly Met  Phe Leu Thr 
    2285                 2290                 2295             


Leu Phe  Val Asn Thr Leu Leu  Asn Ile Thr Ile Ala  Ser Arg Val 
    2300                 2305                 2310             


Leu Glu  Asp Arg Leu Thr Lys  Ser Ala Cys Ala Ala  Phe Ile Gly 
    2315                 2320                 2325             


Asp Asp  Asn Ile Ile His Gly  Val Val Ser Asp Glu  Leu Met Ala 
    2330                 2335                 2340             


Ala Arg  Cys Ala Thr Trp Met  Asn Met Glu Val Lys  Ile Ile Asp 
    2345                 2350                 2355             


Ala Val  Val Ser Leu Lys Ala  Pro Tyr Phe Cys Gly  Gly Phe Ile 
    2360                 2365                 2370             


Leu His  Asp Thr Val Thr Gly  Thr Ala Cys Arg Val  Ala Asp Pro 
    2375                 2380                 2385             


Leu Lys  Arg Leu Phe Lys Leu  Gly Lys Pro Leu Ala  Ala Gly Asp 
    2390                 2395                 2400             


Glu Gln  Asp Glu Asp Arg Arg  Arg Ala Leu Ala Asp  Glu Val Ile 
    2405                 2410                 2415             


Arg Trp  Gln Arg Thr Gly Leu  Ile Asp Glu Leu Glu  Lys Ala Val 
    2420                 2425                 2430             


Tyr Ser  Arg Tyr Glu Val Gln  Gly Ile Ser Val Val  Val Met Ser 
    2435                 2440                 2445             


Met Ala  Thr Phe Ala Ser Ser  Arg Ser Asn Phe Glu  Lys Leu Arg 
    2450                 2455                 2460             


Gly Pro  Val Ile Thr Leu Tyr  Gly Gly Pro Lys 
    2465                 2470                 


<210>  5
<211>  11835
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Polynucleotide sequence of CHIKV LR2006 OPY1 non-structural 
       polyprotein with mutation at position 532 (CHIKV-R532H).

<400>  5
atggctgcgt gagacacacg tagcctacca gtttcttact gctctactct gcaaagcaag       60

agattaataa cccatcatgg atcctgtgta cgtggacata gacgctgaca gcgccttttt      120

gaaggccctg caacgtgcgt accccatgtt tgaggtggaa ccaaggcagg tcacaccgaa      180

tgaccatgct aatgctagag cgttctcgca tctagctata aaactaatag agcaggaaat      240

tgaccccgac tcaaccatcc tggatatcgg cagtgcgcca gcaaggagga tgatgtcgga      300

caggaagtac cactgcgtct gcccgatgcg cagtgcggaa gatcccgaga gactcgccaa      360

ttatgcgaga aagctagcat ctgccgcagg aaaagtcctg gacagaaaca tctctggaaa      420

gatcggggac ttacaagcag taatggccgt gccagacacg gagacgccaa cattctgctt      480

acacacagac gtctcatgta gacagagagc agacgtcgct atataccaag acgtctatgc      540

tgtacacgca cccacgtcgc tataccacca ggcgattaaa ggggtccgag tggcgtactg      600

ggttgggttc gacacaaccc cgttcatgta caatgccatg gcgggtgcct acccctcata      660

ctcgacaaac tgggcagatg agcaggtact gaaggctaag aacataggat tatgttcaac      720

agacctgacg gaaggtagac gaggcaagtt gtctattatg agagggaaaa agctaaaacc      780

gtgcgaccgt gtgctgttct cagtagggtc aacgctctac ccggaaagcc gcaagctact      840

taagagctgg cacctgccat cggtgttcca tttaaagggc aaactcagct tcacatgccg      900

ctgtgataca gtggtttcgt gtgagggcta cgtcgttaag agaataacga tgagcccagg      960

cctttatgga aaaaccacag ggtatgcggt aacccaccac gcagacggat tcctgatgtg     1020

caagactacc gacacggttg acggcgaaag aatgtcattc tcggtgtgca catacgtgcc     1080

ggcgaccatt tgtgatcaaa tgaccggcat ccttgctaca gaagtcacgc cggaggatgc     1140

acagaagctg ttggtggggc tgaaccagag aatagtggtt aacggcagaa cgcaacggaa     1200

tacgaacacc atgaaaaatt atctgcttcc cgtggtcgcc caagccttca gtaagtgggc     1260

aaaggagtgc cggaaagaca tggaagatga aaaactcctg ggggtcagag aaagaacact     1320

gacctgctgc tgtctatggg cattcaagaa gcagaaaaca cacacggtct acaagaggcc     1380

tgatacccag tcaattcaga aggttcaggc cgagtttgac agctttgtgg taccgagtct     1440

gtggtcgtcc gggttgtcaa tccctttgag gactagaatc aaatggttgt taagcaaggt     1500

gccaaaaacc gacctgatcc catacagcgg agacgcccga gaagcccggg acgcagaaaa     1560

agaagcagag gaagaacgag aagcagaact gactcgcgaa gccctaccac ctctacaggc     1620

agcacaggaa gatgttcagg tcgaaatcga cgtggaacag cttgaggacc acgcgggcgc     1680

aggaataata gagactccga gaggagctat caaagttact gcccaaccaa cagaccacgt     1740

cgtgggagag tacctggtac tctccccgca gaccgtacta cgtagccaga agctcagtct     1800

gattcacgct ttggcggagc aagtgaagac gtgcacgcac aacggacgag cagggaggta     1860

tgcggtcgaa gcgtacgacg gccgagtcct agtgccctca ggctatgcaa tctcgcctga     1920

agacttccag agtctaagcg aaagcgcaac gatggtgtat aacgaaagag agttcgtaaa     1980

cagaaagcta caccatattg cgatgcacgg accagccctg aacaccgacg aagagtcgta     2040

tgagctggtg agggcagaga ggacagaaca cgagtacgtc tacgacgtgg atcagagaag     2100

atgctgtaag aaggaagaag ccgcaggact ggtactggtg ggcgacttga ctaatccgcc     2160

ctaccacgaa ttcgcatatg aagggctaaa aatccgccct gcctgcccat acaaaattgc     2220

agtcatagga gtcttcggag taccgggatc tggcaagtca gctattatca agaacctagt     2280

taccaggcag gacctggtga ctagcggaaa gaaagaaaac tgccaagaaa tcaccaccga     2340

cgtgatgaga cagagaggtc tagagatatc tgcacgtacg gttgactcgc tgctcttgaa     2400

tggatgcaac agaccagtcg acgtgttgta cgtagacgag gcgtttgcgt gccactctgg     2460

aacgctactt gctttgatcg ccttggtgag accaaggcag aaagttgtac tttgtggtga     2520

cccgaagcag tgcggcttct tcaatatgat gcagatgaaa gtcaactata atcacaacat     2580

ctgcacccaa gtgtaccaca aaagtatctc caggcggtgt acactgcctg tgaccgccat     2640

tgtgtcatcg ttgcattacg aaggcaaaat gcgcactacg aatgagtaca acaagccgat     2700

tgtagtggac actacaggct caacaaaacc tgaccctgga gacctcgtgt taacgtgctt     2760

cagagggtgg gttaaacaac tgcaaattga ctatcgtgga tacgaggtca tgacagcagc     2820

cgcatcccaa gggttaacca gaaaaggagt ttacgcagtt agacaaaaag ttaatgaaaa     2880

cccgctctat gcatcaacgt cagagcacgt caacgtactc ctaacgcgta cggaaggtaa     2940

actggtatgg aagacacttt ccggcgaccc gtggataaag acgctgcaga acccaccgaa     3000

aggaaacttc aaagcaacta ttaaggagtg ggaggtggag catgcatcaa taatggcggg     3060

catctgcagt caccaaatga ccttcgatac attccaaaat aaagccaacg tttgttgggc     3120

taagagcttg gtccctatcc tcgaaacagc ggggataaaa ctaaatgata ggcagtggtc     3180

tcagataatt caagccttca aagaagacaa agcatactca cctgaagtag ccctgaatga     3240

aatatgtacg cgcatgtatg gggtggatct agacagcggg ctattttcta aaccgttggt     3300

gtctgtgtat tacgcggata accactggga taataggcct ggagggaaaa tgttcggatt     3360

taaccccgag gcagcatcca ttctagaaag aaagtatcca ttcacaaaag ggaagtggaa     3420

catcaacaag cagatctgcg tgactaccag gaggatagaa gactttaacc ctaccaccaa     3480

catcataccg gccaacagga gactaccaca ctcattagtg gccgaacacc gcccagtaaa     3540

aggggaaaga atggaatggc tggttaacaa gataaacggc caccacgtgc tcctggtcag     3600

tggctataac cttgcactgc ctactaagag agtcacttgg gtagcgccgt taggtgtccg     3660

cggagcggac tacacataca acctagagtt gggtctgcca gcaacgcttg gtaggtatga     3720

cctagtggtc ataaacatcc acacaccttt tcgcatacac cattaccaac agtgcgtcga     3780

ccacgcaatg aaactgcaaa tgctcggggg tgactcattg agactgctca aaccgggcgg     3840

ctctctattg atcagagcat atggttacgc agatagaacc agtgaacgag tcatctgcgt     3900

attgggacgc aagtttagat cgtctagagc gttgaaacca ccatgtgtca ccagcaacac     3960

tgagatgttt ttcctattca gcaactttga caatggcaga aggaatttca caactcatgt     4020

catgaacaat caactgaatg cagccttcgt aggacaggtc acccgagcag gatgtgcacc     4080

gtcgtaccgg gtaaaacgca tggacatcgc gaagaacgat gaagagtgcg tagtcaacgc     4140

cgctaaccct cgcgggttac cgggtggcgg tgtttgcaag gcagtataca aaaaatggcc     4200

ggagtccttt aagaacagtg caacaccagt gggaaccgca aaaacagtta tgtgcggtac     4260

gtatccagta atccacgctg ttggaccaaa cttctctaat tattcggagt ctgaagggga     4320

ccgggaattg gcagctgcct atcgagaagt cgcaaaggaa gtaactaggc tgggagtaaa     4380

tagtgtagct atacctctcc tctccacagg tgtatactca ggagggaaag acaggctgac     4440

ccagtcactg aaccacctct ttacagccat ggactcgacg gatgcagacg tggtcatcta     4500

ctgccgcgac aaagaatggg agaagaaaat atctgaggcc atacagatgc ggacccaagt     4560

agagctgctg gatgagcaca tctccataga ctgcgatatt gttcgcgtgc accctgacag     4620

cagcttggca ggcagaaaag gatacagcac cacggaaggc gcactgtact catatctaga     4680

agggacccgt tttcatcaga cggctgtgga tatggcggag atacatacta tgtggccaaa     4740

gcaaacagag gccaatgagc aagtctgcct atatgccctg ggggaaagta ttgaatcgat     4800

caggcagaaa tgcccggtgg atgatgcaga cgcatcatct ccccccaaaa ctgtcccgtg     4860

cctttgccgt tacgctatga ctccagaacg cgtcacccgg cttcgcatga accacgtcac     4920

aagcataatt gtgtgttctt cgtttcccct cccaaagtac aaaatagaag gagtgcaaaa     4980

agtcaaatgc tctaaggtaa tgctatttga ccacaacgtg ccatcgcgcg taagtccaag     5040

ggaatataga tcttcccagg agtctgcaca ggaggcgagt acaatcacgt cactgacgca     5100

tagtcaattc gacctaagcg ttgatggcga gatactgccc gtcccgtcag acctggatgc     5160

tgacgcccca gccctagaac cagcactaga cgacggggcg acacacacgc tgccatccac     5220

aaccggaaac cttgcggccg tgtctgattg ggtaatgagc accgtacctg tcgcgccgcc     5280

cagaagaagg cgagggagaa acctgactgt gacatgtgac gagagagaag ggaatataac     5340

acccatggct agcgtccgat tctttagggc agagctgtgt ccggtcgtac aagaaacagc     5400

ggagacgcgt gacacagcaa tgtctcttca ggcaccaccg agtaccgcca cggaaccgaa     5460

tcatccgccg atctccttcg gagcatcaag cgagacgttc cccattacat ttggggactt     5520

caacgaagga gaaatcgaaa gcttgtcttc tgagctacta actttcggag acttcttacc     5580

aggagaagtg gatgacttga cagacagcga ctggtccacg tgctcagaca cggacgacga     5640

gttaagacta gacagggcag gtgggtatat attctcgtcg gacaccggtc caggtcattt     5700

acaacagaag tcagtacgcc agtcagtgct gccggtgaac accctggagg aagtccacga     5760

ggagaagtgt tacccaccta agctggatga agcaaaggag caactattac ttaagaaact     5820

ccaggagagt gcatccatgg ccaacagaag caggtatcag tcgcgcaaag tagaaaacat     5880

gaaagcagca atcatccaga gactaaagag aggctgtaga ctatacttaa tgtcagagac     5940

cccaaaagtc cctacttacc ggactacata tccggcgcct gtgtactcgc ctccgatcaa     6000

cgtccgattg tccaatcccg agtccgcagt ggcagcatgc aatgagttct tagctagaaa     6060

ctatccaact gtctcatcat accaaattac cgacgagtat gatgcatatc tagacatggt     6120

ggacgggtcg gagagttgcc tggaccgagc gacattcaat ccgtcaaaac tcaggagcta     6180

cccgaaacag cacgcttacc acgcgccctc catcagaagc gctgtaccgt ccccattcca     6240

gaacacacta cagaatgtac tggcagcagc cacgaaaaga aactgcaacg tcacacagat     6300

gagggaatta cccactttgg actcagcagt attcaacgtg gagtgtttca aaaaattcgc     6360

atgcaaccaa gaatactggg aagaatttgc tgccagccct attaggataa caactgagaa     6420

tttagcaacc tatgttacta aactaaaagg gccaaaagca gcagcgctat tcgcaaaaac     6480

ccataatcta ctgccactac aggaagtacc aatggatagg ttcacagtag atatgaaaag     6540

ggacgtaaag gtgactcctg gtacaaagca tacagaggaa agacctaagg tgcaggttat     6600

acaggcggct gaacccttgg cgacagcata cctatgtggg attcacagag agctggttag     6660

gaggctgaac gccgtcctcc tacccaatgt acatacacta tttgacatgt ctgccgagga     6720

tttcgatgcc atcatagccg cacactttaa gccaggagac actgttttgg aaacggacat     6780

agcctccttt gataagagcc aagatgattc acttgcgctt actgctttga tgctgttaga     6840

ggatttaggg gtggatcact ccctgctgga cttgatagag gctgctttcg gagagatttc     6900

cagctgtcac ctaccgacag gtacgcgctt caagttcggc gccatgatga aatcaggtat     6960

gttcctaact ctgttcgtca acacattgtt aaacatcacc atcgccagcc gagtgctgga     7020

agatcgtctg acaaaatccg cgtgcgcggc cttcatcggc gacgacaaca taatacatgg     7080

agtcgtctcc gatgaattga tggcagccag atgtgccact tggatgaaca tggaagtgaa     7140

gatcatagat gcagttgtat ccttgaaagc cccttacttt tgtggagggt ttatactgca     7200

cgatactgtg acaggaacag cttgcagagt ggcagacccg ctaaaaaggc tttttaaact     7260

gggcaaaccg ctagcggcag gtgacgaaca agatgaagat agaagacgag cgctggctga     7320

cgaagtgatc agatggcaac gaacagggct aattgatgag ctggagaaag cggtatactc     7380

taggtacgaa gtgcagggta tatcagttgt ggtaatgtcc atggccacct ttgcaagctc     7440

cagatccaac ttcgagaagc tcagaggacc cgtcataact ttgtacggcg gtcctaaata     7500

ggtacgcact acagctacct attttgcaga agccgacagc aagtatctaa acactaatca     7560

gctacaatgg agttcatccc aacccaaact ttttacaata ggaggtacca gcctcgaccc     7620

tggactccgc gccctactat ccaagtcatc aggcccagac cgcgccctca gaggcaagct     7680

gggcaacttg cccagctgat ctcagcagtt aataaactga caatgcgcgc ggtaccacaa     7740

cagaagccac gcaggaatcg gaagaataag aagcaaaagc aaaaacaaca ggcgccacaa     7800

aacaacacaa atcaaaagaa gcagccacct aaaaagaaac cggctcaaaa gaaaaagaag     7860

ccgggccgca gagagaggat gtgcatgaaa atcgaaaatg attgtatttt cgaagtcaag     7920

cacgaaggta aggtaacagg ttacgcgtgc ctggtggggg acaaagtaat gaaaccagca     7980

cacgtaaagg ggaccatcga taacgcggac ctggccaaac tggcctttaa gcggtcatct     8040

aagtatgacc ttgaatgcgc gcagataccc gtgcacatga agtccgacgc ttcgaagttc     8100

acccatgaga aaccggaggg gtactacaac tggcaccacg gagcagtaca gtactcagga     8160

ggccggttca ccatccctac aggtgctggc aaaccagggg acagcggcag accgatcttc     8220

gacaacaagg gacgcgtggt ggccatagtc ttaggaggag ctaatgaagg agcccgtaca     8280

gccctctcgg tggtgacctg gaataaagac attgtcacta aaatcacccc cgagggggcc     8340

gaagagtgga gtcttgccat cccagttatg tgcctgttgg caaacaccac gttcccctgc     8400

tcccagcccc cttgcacgcc ctgctgctac gaaaaggaac cggaggaaac cctacgcatg     8460

cttgaggaca acgtcatgag acctgggtac tatcagctgc tacaagcatc cttaacatgt     8520

tctccccacc gccagcgacg cagcaccaag gacaacttca atgtctataa agccacaaga     8580

ccatacttag ctcactgtcc cgactgtgga gaagggcact cgtgccatag tcccgtagca     8640

ctagaacgca tcagaaatga agcgacagac gggacgctga aaatccaggt ctccttgcaa     8700

atcggaataa agacggatga cagccacgat tggaccaagc tgcgttatat ggacaaccac     8760

atgccagcag acgcagagag ggcggggcta tttgtaagaa catcagcacc gtgtacgatt     8820

actggaacaa tgggacactt catcctggcc cgatgtccaa aaggggaaac tctgacggtg     8880

ggattcactg acagtaggaa gattagtcac tcatgtacgc acccatttca ccacgaccct     8940

cctgtgatag gtcgggaaaa attccattcc cgaccgcagc acggtaaaga gctaccttgc     9000

agcacgtacg tgcagagcac cgccgcaact accgaggaga tagaggtaca catgccccca     9060

gacacccctg atcgcacatt aatgtcacaa cagtccggca acgtaaagat cacagtcaat     9120

ggccagacgg tgcggtacaa gtgtaattgc ggtggctcaa atgaaggact aacaactaca     9180

gacaaagtga ttaataactg caaggttgat caatgtcatg ccgcggtcac caatcacaaa     9240

aagtggcagt ataactcccc tctggtcccg cgtaatgctg aacttgggga ccgaaaagga     9300

aaaattcaca tcccgtttcc gctggcaaat gtaacatgca gggtgcctaa agcaaggaac     9360

cccaccgtga cgtacgggaa aaaccaagtc atcatgctac tgtatcctga ccacccaaca     9420

ctcctgtcct accggaatat gggagaagaa ccaaactatc aagaagagtg ggtgatgcat     9480

aagaaggaag tcgtgctaac cgtgccgact gaagggctcg aggtcacgtg gggcaacaac     9540

gagccgtata agtattggcc gcagttatct acaaacggta cagcccatgg ccacccgcat     9600

gagataattc tgtattatta tgagctgtac cccactatga ctgtagtagt tgtgtcagtg     9660

gccacgttca tactcctgtc gatggtgggt atggcagcgg ggatgtgcat gtgtgcacga     9720

cgcagatgca tcacaccgta tgaactgaca ccaggagcta ccgtcccttt cctgcttagc     9780

ctaatatgct gcatcagaac agctaaagcg gccacatacc aagaggctgc gatatacctg     9840

tggaacgagc agcaaccttt gttttggcta caagccctta ttccgctggc agccctgatt     9900

gttctatgca actgtctgag actcttacca tgctgctgta aaacgttggc ttttttagcc     9960

gtaatgagcg tcggtgccca cactgtgagc gcgtacgaac acgtaacagt gatcccgaac    10020

acggtgggag taccgtataa gactctagtc aatagacctg gctacagccc catggtattg    10080

gagatggaac tactgtcagt cactttggag ccaacactat cgcttgatta catcacgtgc    10140

gagtacaaaa ccgtcatccc gtctccgtac gtgaagtgct gcggtacagc agagtgcaag    10200

gacaaaaacc tacctgacta cagctgtaag gtcttcaccg gcgtctaccc atttatgtgg    10260

ggcggcgcct actgcttctg cgacgctgaa aacacgcagt tgagcgaagc acacgtggag    10320

aagtccgaat catgcaaaac agaatttgca tcagcataca gggctcatac cgcatctgca    10380

tcagctaagc tccgcgtcct ttaccaagga aataacatca ctgtaactgc ctatgcaaac    10440

ggcgaccatg ccgtcacagt taaggacgcc aaattcattg tggggccaat gtcttcagcc    10500

tggacacctt tcgacaacaa aattgtggtg tacaaaggtg acgtctataa catggactac    10560

ccgccctttg gcgcaggaag accaggacaa tttggcgata tccaaagtcg cacacctgag    10620

agtaaagacg tctatgctaa tacacaactg gtactgcaga gaccggctgt gggtacggta    10680

cacgtgccat actctcaggc accatctggc tttaagtatt ggctaaaaga acgcggggcg    10740

tcgctgcagc acacagcacc atttggctgc caaatagcaa caaacccggt aagagcggtg    10800

aactgcgccg tagggaacat gcccatctcc atcgacatac cggaagcggc cttcactagg    10860

gtcgtcgacg cgccctcttt aacggacatg tcgtgcgagg taccagcctg cacccattcc    10920

tcagactttg ggggcgtcgc cattattaaa tatgcagcca gcaagaaagg caagtgtgcg    10980

gtgcattcga tgactaacgc cgtcactatt cgggaagctg agatagaagt tgaagggaat    11040

tctcagctgc aaatctcttt ctcgacggcc ttagccagcg ccgaattccg cgtacaagtc    11100

tgttctacac aagtacactg tgcagccgag tgccaccccc cgaaggacca catagtcaac    11160

tacccggcgt cacataccac cctcggggtc caggacatct ccgctacggc gatgtcatgg    11220

gtgcagaaga tcacgggagg tgtgggactg gttgttgctg ttgccgcact gattctaatc    11280

gtggtgctat gcgtgtcgtt cagcaggcac taacttgaca attaagtatg aaggtatatg    11340

tgtcccctaa gagacacact gtacatagca aataatctat agatcaaagg gctacgcaac    11400

ccctgaatag taacaaaata caaaatcact aaaaattata aaaacagaaa aatacataaa    11460

taggtatacg tgtcccctaa gagacacatt gtatgtaggt gataagtata gatcaaaggg    11520

ccgaataacc cctgaatagt aacaaaatat gaaaatcaat aaaaatcata aaatagaaaa    11580

accataaaca gaagtagttc aaagggctat aaaacccctg aatagtaaca aaacataaaa    11640

ttaataaaaa tcaaatgaat accataattg gcaaacggaa gagatgtagg tacttaagct    11700

tcctaaaagc agccgaactc actttgagaa gtaggcatag cataccgaac tcttccacga    11760

ttctccgaac ccacagggac gtaggagatg ttattttgtt tttaatattt caaaaaaaaa    11820

aaaaaaaaaa aaaaa                                                     11835


<210>  6
<211>  11835
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Polynucleotide sequence of CHIKV LR2006 OPY1 non-structural 
       polyprotein with mutation at position 1050 (CHIKV-E1050V).

<400>  6
atggctgcgt gagacacacg tagcctacca gtttcttact gctctactct gcaaagcaag       60

agattaataa cccatcatgg atcctgtgta cgtggacata gacgctgaca gcgccttttt      120

gaaggccctg caacgtgcgt accccatgtt tgaggtggaa ccaaggcagg tcacaccgaa      180

tgaccatgct aatgctagag cgttctcgca tctagctata aaactaatag agcaggaaat      240

tgaccccgac tcaaccatcc tggatatcgg cagtgcgcca gcaaggagga tgatgtcgga      300

caggaagtac cactgcgtct gcccgatgcg cagtgcggaa gatcccgaga gactcgccaa      360

ttatgcgaga aagctagcat ctgccgcagg aaaagtcctg gacagaaaca tctctggaaa      420

gatcggggac ttacaagcag taatggccgt gccagacacg gagacgccaa cattctgctt      480

acacacagac gtctcatgta gacagagagc agacgtcgct atataccaag acgtctatgc      540

tgtacacgca cccacgtcgc tataccacca ggcgattaaa ggggtccgag tggcgtactg      600

ggttgggttc gacacaaccc cgttcatgta caatgccatg gcgggtgcct acccctcata      660

ctcgacaaac tgggcagatg agcaggtact gaaggctaag aacataggat tatgttcaac      720

agacctgacg gaaggtagac gaggcaagtt gtctattatg agagggaaaa agctaaaacc      780

gtgcgaccgt gtgctgttct cagtagggtc aacgctctac ccggaaagcc gcaagctact      840

taagagctgg cacctgccat cggtgttcca tttaaagggc aaactcagct tcacatgccg      900

ctgtgataca gtggtttcgt gtgagggcta cgtcgttaag agaataacga tgagcccagg      960

cctttatgga aaaaccacag ggtatgcggt aacccaccac gcagacggat tcctgatgtg     1020

caagactacc gacacggttg acggcgaaag aatgtcattc tcggtgtgca catacgtgcc     1080

ggcgaccatt tgtgatcaaa tgaccggcat ccttgctaca gaagtcacgc cggaggatgc     1140

acagaagctg ttggtggggc tgaaccagag aatagtggtt aacggcagaa cgcaacggaa     1200

tacgaacacc atgaaaaatt atctgcttcc cgtggtcgcc caagccttca gtaagtgggc     1260

aaaggagtgc cggaaagaca tggaagatga aaaactcctg ggggtcagag aaagaacact     1320

gacctgctgc tgtctatggg cattcaagaa gcagaaaaca cacacggtct acaagaggcc     1380

tgatacccag tcaattcaga aggttcaggc cgagtttgac agctttgtgg taccgagtct     1440

gtggtcgtcc gggttgtcaa tccctttgag gactagaatc aaatggttgt taagcaaggt     1500

gccaaaaacc gacctgatcc catacagcgg agacgcccga gaagcccggg acgcagaaaa     1560

agaagcagag gaagaacgag aagcagaact gactcgcgaa gccctaccac ctctacaggc     1620

agcacaggaa gatgttcagg tcgaaatcga cgtggaacag cttgaggaca gagcgggcgc     1680

aggaataata gagactccga gaggagctat caaagttact gcccaaccaa cagaccacgt     1740

cgtgggagag tacctggtac tctccccgca gaccgtacta cgtagccaga agctcagtct     1800

gattcacgct ttggcggagc aagtgaagac gtgcacgcac aacggacgag cagggaggta     1860

tgcggtcgaa gcgtacgacg gccgagtcct agtgccctca ggctatgcaa tctcgcctga     1920

agacttccag agtctaagcg aaagcgcaac gatggtgtat aacgaaagag agttcgtaaa     1980

cagaaagcta caccatattg cgatgcacgg accagccctg aacaccgacg aagagtcgta     2040

tgagctggtg agggcagaga ggacagaaca cgagtacgtc tacgacgtgg atcagagaag     2100

atgctgtaag aaggaagaag ccgcaggact ggtactggtg ggcgacttga ctaatccgcc     2160

ctaccacgaa ttcgcatatg aagggctaaa aatccgccct gcctgcccat acaaaattgc     2220

agtcatagga gtcttcggag taccgggatc tggcaagtca gctattatca agaacctagt     2280

taccaggcag gacctggtga ctagcggaaa gaaagaaaac tgccaagaaa tcaccaccga     2340

cgtgatgaga cagagaggtc tagagatatc tgcacgtacg gttgactcgc tgctcttgaa     2400

tggatgcaac agaccagtcg acgtgttgta cgtagacgag gcgtttgcgt gccactctgg     2460

aacgctactt gctttgatcg ccttggtgag accaaggcag aaagttgtac tttgtggtga     2520

cccgaagcag tgcggcttct tcaatatgat gcagatgaaa gtcaactata atcacaacat     2580

ctgcacccaa gtgtaccaca aaagtatctc caggcggtgt acactgcctg tgaccgccat     2640

tgtgtcatcg ttgcattacg aaggcaaaat gcgcactacg aatgagtaca acaagccgat     2700

tgtagtggac actacaggct caacaaaacc tgaccctgga gacctcgtgt taacgtgctt     2760

cagagggtgg gttaaacaac tgcaaattga ctatcgtgga tacgaggtca tgacagcagc     2820

cgcatcccaa gggttaacca gaaaaggagt ttacgcagtt agacaaaaag ttaatgaaaa     2880

cccgctctat gcatcaacgt cagagcacgt caacgtactc ctaacgcgta cggaaggtaa     2940

actggtatgg aagacacttt ccggcgaccc gtggataaag acgctgcaga acccaccgaa     3000

aggaaacttc aaagcaacta ttaaggagtg ggaggtggag catgcatcaa taatggcggg     3060

catctgcagt caccaaatga ccttcgatac attccaaaat aaagccaacg tttgttgggc     3120

taagagcttg gtccctatcc tcgaaacagc ggggataaaa ctaaatgata ggcagtggtc     3180

tcagataatt caagccttca aagaagacaa agcatactca cctgtggtag ccctgaatga     3240

aatatgtacg cgcatgtatg gggtggatct agacagcggg ctattttcta aaccgttggt     3300

gtctgtgtat tacgcggata accactggga taataggcct ggagggaaaa tgttcggatt     3360

taaccccgag gcagcatcca ttctagaaag aaagtatcca ttcacaaaag ggaagtggaa     3420

catcaacaag cagatctgcg tgactaccag gaggatagaa gactttaacc ctaccaccaa     3480

catcataccg gccaacagga gactaccaca ctcattagtg gccgaacacc gcccagtaaa     3540

aggggaaaga atggaatggc tggttaacaa gataaacggc caccacgtgc tcctggtcag     3600

tggctataac cttgcactgc ctactaagag agtcacttgg gtagcgccgt taggtgtccg     3660

cggagcggac tacacataca acctagagtt gggtctgcca gcaacgcttg gtaggtatga     3720

cctagtggtc ataaacatcc acacaccttt tcgcatacac cattaccaac agtgcgtcga     3780

ccacgcaatg aaactgcaaa tgctcggggg tgactcattg agactgctca aaccgggcgg     3840

ctctctattg atcagagcat atggttacgc agatagaacc agtgaacgag tcatctgcgt     3900

attgggacgc aagtttagat cgtctagagc gttgaaacca ccatgtgtca ccagcaacac     3960

tgagatgttt ttcctattca gcaactttga caatggcaga aggaatttca caactcatgt     4020

catgaacaat caactgaatg cagccttcgt aggacaggtc acccgagcag gatgtgcacc     4080

gtcgtaccgg gtaaaacgca tggacatcgc gaagaacgat gaagagtgcg tagtcaacgc     4140

cgctaaccct cgcgggttac cgggtggcgg tgtttgcaag gcagtataca aaaaatggcc     4200

ggagtccttt aagaacagtg caacaccagt gggaaccgca aaaacagtta tgtgcggtac     4260

gtatccagta atccacgctg ttggaccaaa cttctctaat tattcggagt ctgaagggga     4320

ccgggaattg gcagctgcct atcgagaagt cgcaaaggaa gtaactaggc tgggagtaaa     4380

tagtgtagct atacctctcc tctccacagg tgtatactca ggagggaaag acaggctgac     4440

ccagtcactg aaccacctct ttacagccat ggactcgacg gatgcagacg tggtcatcta     4500

ctgccgcgac aaagaatggg agaagaaaat atctgaggcc atacagatgc ggacccaagt     4560

agagctgctg gatgagcaca tctccataga ctgcgatatt gttcgcgtgc accctgacag     4620

cagcttggca ggcagaaaag gatacagcac cacggaaggc gcactgtact catatctaga     4680

agggacccgt tttcatcaga cggctgtgga tatggcggag atacatacta tgtggccaaa     4740

gcaaacagag gccaatgagc aagtctgcct atatgccctg ggggaaagta ttgaatcgat     4800

caggcagaaa tgcccggtgg atgatgcaga cgcatcatct ccccccaaaa ctgtcccgtg     4860

cctttgccgt tacgctatga ctccagaacg cgtcacccgg cttcgcatga accacgtcac     4920

aagcataatt gtgtgttctt cgtttcccct cccaaagtac aaaatagaag gagtgcaaaa     4980

agtcaaatgc tctaaggtaa tgctatttga ccacaacgtg ccatcgcgcg taagtccaag     5040

ggaatataga tcttcccagg agtctgcaca ggaggcgagt acaatcacgt cactgacgca     5100

tagtcaattc gacctaagcg ttgatggcga gatactgccc gtcccgtcag acctggatgc     5160

tgacgcccca gccctagaac cagcactaga cgacggggcg acacacacgc tgccatccac     5220

aaccggaaac cttgcggccg tgtctgattg ggtaatgagc accgtacctg tcgcgccgcc     5280

cagaagaagg cgagggagaa acctgactgt gacatgtgac gagagagaag ggaatataac     5340

acccatggct agcgtccgat tctttagggc agagctgtgt ccggtcgtac aagaaacagc     5400

ggagacgcgt gacacagcaa tgtctcttca ggcaccaccg agtaccgcca cggaaccgaa     5460

tcatccgccg atctccttcg gagcatcaag cgagacgttc cccattacat ttggggactt     5520

caacgaagga gaaatcgaaa gcttgtcttc tgagctacta actttcggag acttcttacc     5580

aggagaagtg gatgacttga cagacagcga ctggtccacg tgctcagaca cggacgacga     5640

gttaagacta gacagggcag gtgggtatat attctcgtcg gacaccggtc caggtcattt     5700

acaacagaag tcagtacgcc agtcagtgct gccggtgaac accctggagg aagtccacga     5760

ggagaagtgt tacccaccta agctggatga agcaaaggag caactattac ttaagaaact     5820

ccaggagagt gcatccatgg ccaacagaag caggtatcag tcgcgcaaag tagaaaacat     5880

gaaagcagca atcatccaga gactaaagag aggctgtaga ctatacttaa tgtcagagac     5940

cccaaaagtc cctacttacc ggactacata tccggcgcct gtgtactcgc ctccgatcaa     6000

cgtccgattg tccaatcccg agtccgcagt ggcagcatgc aatgagttct tagctagaaa     6060

ctatccaact gtctcatcat accaaattac cgacgagtat gatgcatatc tagacatggt     6120

ggacgggtcg gagagttgcc tggaccgagc gacattcaat ccgtcaaaac tcaggagcta     6180

cccgaaacag cacgcttacc acgcgccctc catcagaagc gctgtaccgt ccccattcca     6240

gaacacacta cagaatgtac tggcagcagc cacgaaaaga aactgcaacg tcacacagat     6300

gagggaatta cccactttgg actcagcagt attcaacgtg gagtgtttca aaaaattcgc     6360

atgcaaccaa gaatactggg aagaatttgc tgccagccct attaggataa caactgagaa     6420

tttagcaacc tatgttacta aactaaaagg gccaaaagca gcagcgctat tcgcaaaaac     6480

ccataatcta ctgccactac aggaagtacc aatggatagg ttcacagtag atatgaaaag     6540

ggacgtaaag gtgactcctg gtacaaagca tacagaggaa agacctaagg tgcaggttat     6600

acaggcggct gaacccttgg cgacagcata cctatgtggg attcacagag agctggttag     6660

gaggctgaac gccgtcctcc tacccaatgt acatacacta tttgacatgt ctgccgagga     6720

tttcgatgcc atcatagccg cacactttaa gccaggagac actgttttgg aaacggacat     6780

agcctccttt gataagagcc aagatgattc acttgcgctt actgctttga tgctgttaga     6840

ggatttaggg gtggatcact ccctgctgga cttgatagag gctgctttcg gagagatttc     6900

cagctgtcac ctaccgacag gtacgcgctt caagttcggc gccatgatga aatcaggtat     6960

gttcctaact ctgttcgtca acacattgtt aaacatcacc atcgccagcc gagtgctgga     7020

agatcgtctg acaaaatccg cgtgcgcggc cttcatcggc gacgacaaca taatacatgg     7080

agtcgtctcc gatgaattga tggcagccag atgtgccact tggatgaaca tggaagtgaa     7140

gatcatagat gcagttgtat ccttgaaagc cccttacttt tgtggagggt ttatactgca     7200

cgatactgtg acaggaacag cttgcagagt ggcagacccg ctaaaaaggc tttttaaact     7260

gggcaaaccg ctagcggcag gtgacgaaca agatgaagat agaagacgag cgctggctga     7320

cgaagtgatc agatggcaac gaacagggct aattgatgag ctggagaaag cggtatactc     7380

taggtacgaa gtgcagggta tatcagttgt ggtaatgtcc atggccacct ttgcaagctc     7440

cagatccaac ttcgagaagc tcagaggacc cgtcataact ttgtacggcg gtcctaaata     7500

ggtacgcact acagctacct attttgcaga agccgacagc aagtatctaa acactaatca     7560

gctacaatgg agttcatccc aacccaaact ttttacaata ggaggtacca gcctcgaccc     7620

tggactccgc gccctactat ccaagtcatc aggcccagac cgcgccctca gaggcaagct     7680

gggcaacttg cccagctgat ctcagcagtt aataaactga caatgcgcgc ggtaccacaa     7740

cagaagccac gcaggaatcg gaagaataag aagcaaaagc aaaaacaaca ggcgccacaa     7800

aacaacacaa atcaaaagaa gcagccacct aaaaagaaac cggctcaaaa gaaaaagaag     7860

ccgggccgca gagagaggat gtgcatgaaa atcgaaaatg attgtatttt cgaagtcaag     7920

cacgaaggta aggtaacagg ttacgcgtgc ctggtggggg acaaagtaat gaaaccagca     7980

cacgtaaagg ggaccatcga taacgcggac ctggccaaac tggcctttaa gcggtcatct     8040

aagtatgacc ttgaatgcgc gcagataccc gtgcacatga agtccgacgc ttcgaagttc     8100

acccatgaga aaccggaggg gtactacaac tggcaccacg gagcagtaca gtactcagga     8160

ggccggttca ccatccctac aggtgctggc aaaccagggg acagcggcag accgatcttc     8220

gacaacaagg gacgcgtggt ggccatagtc ttaggaggag ctaatgaagg agcccgtaca     8280

gccctctcgg tggtgacctg gaataaagac attgtcacta aaatcacccc cgagggggcc     8340

gaagagtgga gtcttgccat cccagttatg tgcctgttgg caaacaccac gttcccctgc     8400

tcccagcccc cttgcacgcc ctgctgctac gaaaaggaac cggaggaaac cctacgcatg     8460

cttgaggaca acgtcatgag acctgggtac tatcagctgc tacaagcatc cttaacatgt     8520

tctccccacc gccagcgacg cagcaccaag gacaacttca atgtctataa agccacaaga     8580

ccatacttag ctcactgtcc cgactgtgga gaagggcact cgtgccatag tcccgtagca     8640

ctagaacgca tcagaaatga agcgacagac gggacgctga aaatccaggt ctccttgcaa     8700

atcggaataa agacggatga cagccacgat tggaccaagc tgcgttatat ggacaaccac     8760

atgccagcag acgcagagag ggcggggcta tttgtaagaa catcagcacc gtgtacgatt     8820

actggaacaa tgggacactt catcctggcc cgatgtccaa aaggggaaac tctgacggtg     8880

ggattcactg acagtaggaa gattagtcac tcatgtacgc acccatttca ccacgaccct     8940

cctgtgatag gtcgggaaaa attccattcc cgaccgcagc acggtaaaga gctaccttgc     9000

agcacgtacg tgcagagcac cgccgcaact accgaggaga tagaggtaca catgccccca     9060

gacacccctg atcgcacatt aatgtcacaa cagtccggca acgtaaagat cacagtcaat     9120

ggccagacgg tgcggtacaa gtgtaattgc ggtggctcaa atgaaggact aacaactaca     9180

gacaaagtga ttaataactg caaggttgat caatgtcatg ccgcggtcac caatcacaaa     9240

aagtggcagt ataactcccc tctggtcccg cgtaatgctg aacttgggga ccgaaaagga     9300

aaaattcaca tcccgtttcc gctggcaaat gtaacatgca gggtgcctaa agcaaggaac     9360

cccaccgtga cgtacgggaa aaaccaagtc atcatgctac tgtatcctga ccacccaaca     9420

ctcctgtcct accggaatat gggagaagaa ccaaactatc aagaagagtg ggtgatgcat     9480

aagaaggaag tcgtgctaac cgtgccgact gaagggctcg aggtcacgtg gggcaacaac     9540

gagccgtata agtattggcc gcagttatct acaaacggta cagcccatgg ccacccgcat     9600

gagataattc tgtattatta tgagctgtac cccactatga ctgtagtagt tgtgtcagtg     9660

gccacgttca tactcctgtc gatggtgggt atggcagcgg ggatgtgcat gtgtgcacga     9720

cgcagatgca tcacaccgta tgaactgaca ccaggagcta ccgtcccttt cctgcttagc     9780

ctaatatgct gcatcagaac agctaaagcg gccacatacc aagaggctgc gatatacctg     9840

tggaacgagc agcaaccttt gttttggcta caagccctta ttccgctggc agccctgatt     9900

gttctatgca actgtctgag actcttacca tgctgctgta aaacgttggc ttttttagcc     9960

gtaatgagcg tcggtgccca cactgtgagc gcgtacgaac acgtaacagt gatcccgaac    10020

acggtgggag taccgtataa gactctagtc aatagacctg gctacagccc catggtattg    10080

gagatggaac tactgtcagt cactttggag ccaacactat cgcttgatta catcacgtgc    10140

gagtacaaaa ccgtcatccc gtctccgtac gtgaagtgct gcggtacagc agagtgcaag    10200

gacaaaaacc tacctgacta cagctgtaag gtcttcaccg gcgtctaccc atttatgtgg    10260

ggcggcgcct actgcttctg cgacgctgaa aacacgcagt tgagcgaagc acacgtggag    10320

aagtccgaat catgcaaaac agaatttgca tcagcataca gggctcatac cgcatctgca    10380

tcagctaagc tccgcgtcct ttaccaagga aataacatca ctgtaactgc ctatgcaaac    10440

ggcgaccatg ccgtcacagt taaggacgcc aaattcattg tggggccaat gtcttcagcc    10500

tggacacctt tcgacaacaa aattgtggtg tacaaaggtg acgtctataa catggactac    10560

ccgccctttg gcgcaggaag accaggacaa tttggcgata tccaaagtcg cacacctgag    10620

agtaaagacg tctatgctaa tacacaactg gtactgcaga gaccggctgt gggtacggta    10680

cacgtgccat actctcaggc accatctggc tttaagtatt ggctaaaaga acgcggggcg    10740

tcgctgcagc acacagcacc atttggctgc caaatagcaa caaacccggt aagagcggtg    10800

aactgcgccg tagggaacat gcccatctcc atcgacatac cggaagcggc cttcactagg    10860

gtcgtcgacg cgccctcttt aacggacatg tcgtgcgagg taccagcctg cacccattcc    10920

tcagactttg ggggcgtcgc cattattaaa tatgcagcca gcaagaaagg caagtgtgcg    10980

gtgcattcga tgactaacgc cgtcactatt cgggaagctg agatagaagt tgaagggaat    11040

tctcagctgc aaatctcttt ctcgacggcc ttagccagcg ccgaattccg cgtacaagtc    11100

tgttctacac aagtacactg tgcagccgag tgccaccccc cgaaggacca catagtcaac    11160

tacccggcgt cacataccac cctcggggtc caggacatct ccgctacggc gatgtcatgg    11220

gtgcagaaga tcacgggagg tgtgggactg gttgttgctg ttgccgcact gattctaatc    11280

gtggtgctat gcgtgtcgtt cagcaggcac taacttgaca attaagtatg aaggtatatg    11340

tgtcccctaa gagacacact gtacatagca aataatctat agatcaaagg gctacgcaac    11400

ccctgaatag taacaaaata caaaatcact aaaaattata aaaacagaaa aatacataaa    11460

taggtatacg tgtcccctaa gagacacatt gtatgtaggt gataagtata gatcaaaggg    11520

ccgaataacc cctgaatagt aacaaaatat gaaaatcaat aaaaatcata aaatagaaaa    11580

accataaaca gaagtagttc aaagggctat aaaacccctg aatagtaaca aaacataaaa    11640

ttaataaaaa tcaaatgaat accataattg gcaaacggaa gagatgtagg tacttaagct    11700

tcctaaaagc agccgaactc actttgagaa gtaggcatag cataccgaac tcttccacga    11760

ttctccgaac ccacagggac gtaggagatg ttattttgtt tttaatattt caaaaaaaaa    11820

aaaaaaaaaa aaaaa                                                     11835


<210>  7
<211>  11835
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Polynucleotide sequence of CHIKV LR2006 OPY1 non-structural 
       polyprotein with mutations at 532 and 1050 (CHIKV-R532H+E1050V).

<400>  7
atggctgcgt gagacacacg tagcctacca gtttcttact gctctactct gcaaagcaag       60

agattaataa cccatcatgg atcctgtgta cgtggacata gacgctgaca gcgccttttt      120

gaaggccctg caacgtgcgt accccatgtt tgaggtggaa ccaaggcagg tcacaccgaa      180

tgaccatgct aatgctagag cgttctcgca tctagctata aaactaatag agcaggaaat      240

tgaccccgac tcaaccatcc tggatatcgg cagtgcgcca gcaaggagga tgatgtcgga      300

caggaagtac cactgcgtct gcccgatgcg cagtgcggaa gatcccgaga gactcgccaa      360

ttatgcgaga aagctagcat ctgccgcagg aaaagtcctg gacagaaaca tctctggaaa      420

gatcggggac ttacaagcag taatggccgt gccagacacg gagacgccaa cattctgctt      480

acacacagac gtctcatgta gacagagagc agacgtcgct atataccaag acgtctatgc      540

tgtacacgca cccacgtcgc tataccacca ggcgattaaa ggggtccgag tggcgtactg      600

ggttgggttc gacacaaccc cgttcatgta caatgccatg gcgggtgcct acccctcata      660

ctcgacaaac tgggcagatg agcaggtact gaaggctaag aacataggat tatgttcaac      720

agacctgacg gaaggtagac gaggcaagtt gtctattatg agagggaaaa agctaaaacc      780

gtgcgaccgt gtgctgttct cagtagggtc aacgctctac ccggaaagcc gcaagctact      840

taagagctgg cacctgccat cggtgttcca tttaaagggc aaactcagct tcacatgccg      900

ctgtgataca gtggtttcgt gtgagggcta cgtcgttaag agaataacga tgagcccagg      960

cctttatgga aaaaccacag ggtatgcggt aacccaccac gcagacggat tcctgatgtg     1020

caagactacc gacacggttg acggcgaaag aatgtcattc tcggtgtgca catacgtgcc     1080

ggcgaccatt tgtgatcaaa tgaccggcat ccttgctaca gaagtcacgc cggaggatgc     1140

acagaagctg ttggtggggc tgaaccagag aatagtggtt aacggcagaa cgcaacggaa     1200

tacgaacacc atgaaaaatt atctgcttcc cgtggtcgcc caagccttca gtaagtgggc     1260

aaaggagtgc cggaaagaca tggaagatga aaaactcctg ggggtcagag aaagaacact     1320

gacctgctgc tgtctatggg cattcaagaa gcagaaaaca cacacggtct acaagaggcc     1380

tgatacccag tcaattcaga aggttcaggc cgagtttgac agctttgtgg taccgagtct     1440

gtggtcgtcc gggttgtcaa tccctttgag gactagaatc aaatggttgt taagcaaggt     1500

gccaaaaacc gacctgatcc catacagcgg agacgcccga gaagcccggg acgcagaaaa     1560

agaagcagag gaagaacgag aagcagaact gactcgcgaa gccctaccac ctctacaggc     1620

agcacaggaa gatgttcagg tcgaaatcga cgtggaacag cttgaggacc acgcgggcgc     1680

aggaataata gagactccga gaggagctat caaagttact gcccaaccaa cagaccacgt     1740

cgtgggagag tacctggtac tctccccgca gaccgtacta cgtagccaga agctcagtct     1800

gattcacgct ttggcggagc aagtgaagac gtgcacgcac aacggacgag cagggaggta     1860

tgcggtcgaa gcgtacgacg gccgagtcct agtgccctca ggctatgcaa tctcgcctga     1920

agacttccag agtctaagcg aaagcgcaac gatggtgtat aacgaaagag agttcgtaaa     1980

cagaaagcta caccatattg cgatgcacgg accagccctg aacaccgacg aagagtcgta     2040

tgagctggtg agggcagaga ggacagaaca cgagtacgtc tacgacgtgg atcagagaag     2100

atgctgtaag aaggaagaag ccgcaggact ggtactggtg ggcgacttga ctaatccgcc     2160

ctaccacgaa ttcgcatatg aagggctaaa aatccgccct gcctgcccat acaaaattgc     2220

agtcatagga gtcttcggag taccgggatc tggcaagtca gctattatca agaacctagt     2280

taccaggcag gacctggtga ctagcggaaa gaaagaaaac tgccaagaaa tcaccaccga     2340

cgtgatgaga cagagaggtc tagagatatc tgcacgtacg gttgactcgc tgctcttgaa     2400

tggatgcaac agaccagtcg acgtgttgta cgtagacgag gcgtttgcgt gccactctgg     2460

aacgctactt gctttgatcg ccttggtgag accaaggcag aaagttgtac tttgtggtga     2520

cccgaagcag tgcggcttct tcaatatgat gcagatgaaa gtcaactata atcacaacat     2580

ctgcacccaa gtgtaccaca aaagtatctc caggcggtgt acactgcctg tgaccgccat     2640

tgtgtcatcg ttgcattacg aaggcaaaat gcgcactacg aatgagtaca acaagccgat     2700

tgtagtggac actacaggct caacaaaacc tgaccctgga gacctcgtgt taacgtgctt     2760

cagagggtgg gttaaacaac tgcaaattga ctatcgtgga tacgaggtca tgacagcagc     2820

cgcatcccaa gggttaacca gaaaaggagt ttacgcagtt agacaaaaag ttaatgaaaa     2880

cccgctctat gcatcaacgt cagagcacgt caacgtactc ctaacgcgta cggaaggtaa     2940

actggtatgg aagacacttt ccggcgaccc gtggataaag acgctgcaga acccaccgaa     3000

aggaaacttc aaagcaacta ttaaggagtg ggaggtggag catgcatcaa taatggcggg     3060

catctgcagt caccaaatga ccttcgatac attccaaaat aaagccaacg tttgttgggc     3120

taagagcttg gtccctatcc tcgaaacagc ggggataaaa ctaaatgata ggcagtggtc     3180

tcagataatt caagccttca aagaagacaa agcatactca cctgtggtag ccctgaatga     3240

aatatgtacg cgcatgtatg gggtggatct agacagcggg ctattttcta aaccgttggt     3300

gtctgtgtat tacgcggata accactggga taataggcct ggagggaaaa tgttcggatt     3360

taaccccgag gcagcatcca ttctagaaag aaagtatcca ttcacaaaag ggaagtggaa     3420

catcaacaag cagatctgcg tgactaccag gaggatagaa gactttaacc ctaccaccaa     3480

catcataccg gccaacagga gactaccaca ctcattagtg gccgaacacc gcccagtaaa     3540

aggggaaaga atggaatggc tggttaacaa gataaacggc caccacgtgc tcctggtcag     3600

tggctataac cttgcactgc ctactaagag agtcacttgg gtagcgccgt taggtgtccg     3660

cggagcggac tacacataca acctagagtt gggtctgcca gcaacgcttg gtaggtatga     3720

cctagtggtc ataaacatcc acacaccttt tcgcatacac cattaccaac agtgcgtcga     3780

ccacgcaatg aaactgcaaa tgctcggggg tgactcattg agactgctca aaccgggcgg     3840

ctctctattg atcagagcat atggttacgc agatagaacc agtgaacgag tcatctgcgt     3900

attgggacgc aagtttagat cgtctagagc gttgaaacca ccatgtgtca ccagcaacac     3960

tgagatgttt ttcctattca gcaactttga caatggcaga aggaatttca caactcatgt     4020

catgaacaat caactgaatg cagccttcgt aggacaggtc acccgagcag gatgtgcacc     4080

gtcgtaccgg gtaaaacgca tggacatcgc gaagaacgat gaagagtgcg tagtcaacgc     4140

cgctaaccct cgcgggttac cgggtggcgg tgtttgcaag gcagtataca aaaaatggcc     4200

ggagtccttt aagaacagtg caacaccagt gggaaccgca aaaacagtta tgtgcggtac     4260

gtatccagta atccacgctg ttggaccaaa cttctctaat tattcggagt ctgaagggga     4320

ccgggaattg gcagctgcct atcgagaagt cgcaaaggaa gtaactaggc tgggagtaaa     4380

tagtgtagct atacctctcc tctccacagg tgtatactca ggagggaaag acaggctgac     4440

ccagtcactg aaccacctct ttacagccat ggactcgacg gatgcagacg tggtcatcta     4500

ctgccgcgac aaagaatggg agaagaaaat atctgaggcc atacagatgc ggacccaagt     4560

agagctgctg gatgagcaca tctccataga ctgcgatatt gttcgcgtgc accctgacag     4620

cagcttggca ggcagaaaag gatacagcac cacggaaggc gcactgtact catatctaga     4680

agggacccgt tttcatcaga cggctgtgga tatggcggag atacatacta tgtggccaaa     4740

gcaaacagag gccaatgagc aagtctgcct atatgccctg ggggaaagta ttgaatcgat     4800

caggcagaaa tgcccggtgg atgatgcaga cgcatcatct ccccccaaaa ctgtcccgtg     4860

cctttgccgt tacgctatga ctccagaacg cgtcacccgg cttcgcatga accacgtcac     4920

aagcataatt gtgtgttctt cgtttcccct cccaaagtac aaaatagaag gagtgcaaaa     4980

agtcaaatgc tctaaggtaa tgctatttga ccacaacgtg ccatcgcgcg taagtccaag     5040

ggaatataga tcttcccagg agtctgcaca ggaggcgagt acaatcacgt cactgacgca     5100

tagtcaattc gacctaagcg ttgatggcga gatactgccc gtcccgtcag acctggatgc     5160

tgacgcccca gccctagaac cagcactaga cgacggggcg acacacacgc tgccatccac     5220

aaccggaaac cttgcggccg tgtctgattg ggtaatgagc accgtacctg tcgcgccgcc     5280

cagaagaagg cgagggagaa acctgactgt gacatgtgac gagagagaag ggaatataac     5340

acccatggct agcgtccgat tctttagggc agagctgtgt ccggtcgtac aagaaacagc     5400

ggagacgcgt gacacagcaa tgtctcttca ggcaccaccg agtaccgcca cggaaccgaa     5460

tcatccgccg atctccttcg gagcatcaag cgagacgttc cccattacat ttggggactt     5520

caacgaagga gaaatcgaaa gcttgtcttc tgagctacta actttcggag acttcttacc     5580

aggagaagtg gatgacttga cagacagcga ctggtccacg tgctcagaca cggacgacga     5640

gttaagacta gacagggcag gtgggtatat attctcgtcg gacaccggtc caggtcattt     5700

acaacagaag tcagtacgcc agtcagtgct gccggtgaac accctggagg aagtccacga     5760

ggagaagtgt tacccaccta agctggatga agcaaaggag caactattac ttaagaaact     5820

ccaggagagt gcatccatgg ccaacagaag caggtatcag tcgcgcaaag tagaaaacat     5880

gaaagcagca atcatccaga gactaaagag aggctgtaga ctatacttaa tgtcagagac     5940

cccaaaagtc cctacttacc ggactacata tccggcgcct gtgtactcgc ctccgatcaa     6000

cgtccgattg tccaatcccg agtccgcagt ggcagcatgc aatgagttct tagctagaaa     6060

ctatccaact gtctcatcat accaaattac cgacgagtat gatgcatatc tagacatggt     6120

ggacgggtcg gagagttgcc tggaccgagc gacattcaat ccgtcaaaac tcaggagcta     6180

cccgaaacag cacgcttacc acgcgccctc catcagaagc gctgtaccgt ccccattcca     6240

gaacacacta cagaatgtac tggcagcagc cacgaaaaga aactgcaacg tcacacagat     6300

gagggaatta cccactttgg actcagcagt attcaacgtg gagtgtttca aaaaattcgc     6360

atgcaaccaa gaatactggg aagaatttgc tgccagccct attaggataa caactgagaa     6420

tttagcaacc tatgttacta aactaaaagg gccaaaagca gcagcgctat tcgcaaaaac     6480

ccataatcta ctgccactac aggaagtacc aatggatagg ttcacagtag atatgaaaag     6540

ggacgtaaag gtgactcctg gtacaaagca tacagaggaa agacctaagg tgcaggttat     6600

acaggcggct gaacccttgg cgacagcata cctatgtggg attcacagag agctggttag     6660

gaggctgaac gccgtcctcc tacccaatgt acatacacta tttgacatgt ctgccgagga     6720

tttcgatgcc atcatagccg cacactttaa gccaggagac actgttttgg aaacggacat     6780

agcctccttt gataagagcc aagatgattc acttgcgctt actgctttga tgctgttaga     6840

ggatttaggg gtggatcact ccctgctgga cttgatagag gctgctttcg gagagatttc     6900

cagctgtcac ctaccgacag gtacgcgctt caagttcggc gccatgatga aatcaggtat     6960

gttcctaact ctgttcgtca acacattgtt aaacatcacc atcgccagcc gagtgctgga     7020

agatcgtctg acaaaatccg cgtgcgcggc cttcatcggc gacgacaaca taatacatgg     7080

agtcgtctcc gatgaattga tggcagccag atgtgccact tggatgaaca tggaagtgaa     7140

gatcatagat gcagttgtat ccttgaaagc cccttacttt tgtggagggt ttatactgca     7200

cgatactgtg acaggaacag cttgcagagt ggcagacccg ctaaaaaggc tttttaaact     7260

gggcaaaccg ctagcggcag gtgacgaaca agatgaagat agaagacgag cgctggctga     7320

cgaagtgatc agatggcaac gaacagggct aattgatgag ctggagaaag cggtatactc     7380

taggtacgaa gtgcagggta tatcagttgt ggtaatgtcc atggccacct ttgcaagctc     7440

cagatccaac ttcgagaagc tcagaggacc cgtcataact ttgtacggcg gtcctaaata     7500

ggtacgcact acagctacct attttgcaga agccgacagc aagtatctaa acactaatca     7560

gctacaatgg agttcatccc aacccaaact ttttacaata ggaggtacca gcctcgaccc     7620

tggactccgc gccctactat ccaagtcatc aggcccagac cgcgccctca gaggcaagct     7680

gggcaacttg cccagctgat ctcagcagtt aataaactga caatgcgcgc ggtaccacaa     7740

cagaagccac gcaggaatcg gaagaataag aagcaaaagc aaaaacaaca ggcgccacaa     7800

aacaacacaa atcaaaagaa gcagccacct aaaaagaaac cggctcaaaa gaaaaagaag     7860

ccgggccgca gagagaggat gtgcatgaaa atcgaaaatg attgtatttt cgaagtcaag     7920

cacgaaggta aggtaacagg ttacgcgtgc ctggtggggg acaaagtaat gaaaccagca     7980

cacgtaaagg ggaccatcga taacgcggac ctggccaaac tggcctttaa gcggtcatct     8040

aagtatgacc ttgaatgcgc gcagataccc gtgcacatga agtccgacgc ttcgaagttc     8100

acccatgaga aaccggaggg gtactacaac tggcaccacg gagcagtaca gtactcagga     8160

ggccggttca ccatccctac aggtgctggc aaaccagggg acagcggcag accgatcttc     8220

gacaacaagg gacgcgtggt ggccatagtc ttaggaggag ctaatgaagg agcccgtaca     8280

gccctctcgg tggtgacctg gaataaagac attgtcacta aaatcacccc cgagggggcc     8340

gaagagtgga gtcttgccat cccagttatg tgcctgttgg caaacaccac gttcccctgc     8400

tcccagcccc cttgcacgcc ctgctgctac gaaaaggaac cggaggaaac cctacgcatg     8460

cttgaggaca acgtcatgag acctgggtac tatcagctgc tacaagcatc cttaacatgt     8520

tctccccacc gccagcgacg cagcaccaag gacaacttca atgtctataa agccacaaga     8580

ccatacttag ctcactgtcc cgactgtgga gaagggcact cgtgccatag tcccgtagca     8640

ctagaacgca tcagaaatga agcgacagac gggacgctga aaatccaggt ctccttgcaa     8700

atcggaataa agacggatga cagccacgat tggaccaagc tgcgttatat ggacaaccac     8760

atgccagcag acgcagagag ggcggggcta tttgtaagaa catcagcacc gtgtacgatt     8820

actggaacaa tgggacactt catcctggcc cgatgtccaa aaggggaaac tctgacggtg     8880

ggattcactg acagtaggaa gattagtcac tcatgtacgc acccatttca ccacgaccct     8940

cctgtgatag gtcgggaaaa attccattcc cgaccgcagc acggtaaaga gctaccttgc     9000

agcacgtacg tgcagagcac cgccgcaact accgaggaga tagaggtaca catgccccca     9060

gacacccctg atcgcacatt aatgtcacaa cagtccggca acgtaaagat cacagtcaat     9120

ggccagacgg tgcggtacaa gtgtaattgc ggtggctcaa atgaaggact aacaactaca     9180

gacaaagtga ttaataactg caaggttgat caatgtcatg ccgcggtcac caatcacaaa     9240

aagtggcagt ataactcccc tctggtcccg cgtaatgctg aacttgggga ccgaaaagga     9300

aaaattcaca tcccgtttcc gctggcaaat gtaacatgca gggtgcctaa agcaaggaac     9360

cccaccgtga cgtacgggaa aaaccaagtc atcatgctac tgtatcctga ccacccaaca     9420

ctcctgtcct accggaatat gggagaagaa ccaaactatc aagaagagtg ggtgatgcat     9480

aagaaggaag tcgtgctaac cgtgccgact gaagggctcg aggtcacgtg gggcaacaac     9540

gagccgtata agtattggcc gcagttatct acaaacggta cagcccatgg ccacccgcat     9600

gagataattc tgtattatta tgagctgtac cccactatga ctgtagtagt tgtgtcagtg     9660

gccacgttca tactcctgtc gatggtgggt atggcagcgg ggatgtgcat gtgtgcacga     9720

cgcagatgca tcacaccgta tgaactgaca ccaggagcta ccgtcccttt cctgcttagc     9780

ctaatatgct gcatcagaac agctaaagcg gccacatacc aagaggctgc gatatacctg     9840

tggaacgagc agcaaccttt gttttggcta caagccctta ttccgctggc agccctgatt     9900

gttctatgca actgtctgag actcttacca tgctgctgta aaacgttggc ttttttagcc     9960

gtaatgagcg tcggtgccca cactgtgagc gcgtacgaac acgtaacagt gatcccgaac    10020

acggtgggag taccgtataa gactctagtc aatagacctg gctacagccc catggtattg    10080

gagatggaac tactgtcagt cactttggag ccaacactat cgcttgatta catcacgtgc    10140

gagtacaaaa ccgtcatccc gtctccgtac gtgaagtgct gcggtacagc agagtgcaag    10200

gacaaaaacc tacctgacta cagctgtaag gtcttcaccg gcgtctaccc atttatgtgg    10260

ggcggcgcct actgcttctg cgacgctgaa aacacgcagt tgagcgaagc acacgtggag    10320

aagtccgaat catgcaaaac agaatttgca tcagcataca gggctcatac cgcatctgca    10380

tcagctaagc tccgcgtcct ttaccaagga aataacatca ctgtaactgc ctatgcaaac    10440

ggcgaccatg ccgtcacagt taaggacgcc aaattcattg tggggccaat gtcttcagcc    10500

tggacacctt tcgacaacaa aattgtggtg tacaaaggtg acgtctataa catggactac    10560

ccgccctttg gcgcaggaag accaggacaa tttggcgata tccaaagtcg cacacctgag    10620

agtaaagacg tctatgctaa tacacaactg gtactgcaga gaccggctgt gggtacggta    10680

cacgtgccat actctcaggc accatctggc tttaagtatt ggctaaaaga acgcggggcg    10740

tcgctgcagc acacagcacc atttggctgc caaatagcaa caaacccggt aagagcggtg    10800

aactgcgccg tagggaacat gcccatctcc atcgacatac cggaagcggc cttcactagg    10860

gtcgtcgacg cgccctcttt aacggacatg tcgtgcgagg taccagcctg cacccattcc    10920

tcagactttg ggggcgtcgc cattattaaa tatgcagcca gcaagaaagg caagtgtgcg    10980

gtgcattcga tgactaacgc cgtcactatt cgggaagctg agatagaagt tgaagggaat    11040

tctcagctgc aaatctcttt ctcgacggcc ttagccagcg ccgaattccg cgtacaagtc    11100

tgttctacac aagtacactg tgcagccgag tgccaccccc cgaaggacca catagtcaac    11160

tacccggcgt cacataccac cctcggggtc caggacatct ccgctacggc gatgtcatgg    11220

gtgcagaaga tcacgggagg tgtgggactg gttgttgctg ttgccgcact gattctaatc    11280

gtggtgctat gcgtgtcgtt cagcaggcac taacttgaca attaagtatg aaggtatatg    11340

tgtcccctaa gagacacact gtacatagca aataatctat agatcaaagg gctacgcaac    11400

ccctgaatag taacaaaata caaaatcact aaaaattata aaaacagaaa aatacataaa    11460

taggtatacg tgtcccctaa gagacacatt gtatgtaggt gataagtata gatcaaaggg    11520

ccgaataacc cctgaatagt aacaaaatat gaaaatcaat aaaaatcata aaatagaaaa    11580

accataaaca gaagtagttc aaagggctat aaaacccctg aatagtaaca aaacataaaa    11640

ttaataaaaa tcaaatgaat accataattg gcaaacggaa gagatgtagg tacttaagct    11700

tcctaaaagc agccgaactc actttgagaa gtaggcatag cataccgaac tcttccacga    11760

ttctccgaac ccacagggac gtaggagatg ttattttgtt tttaatattt caaaaaaaaa    11820

aaaaaaaaaa aaaaa                                                     11835


