                         SEQUENCE LISTING

<110>  INSTITUT PASTEUR
 
<120>  METHOD FOR THE IDENTIFICATION OF MUTATED PROTEINS HAVING MODIFIED
        THERMAL STABILITY

<130>  B12327A/AD/HLQ

<140>  PCT/FR2018/xxxxx
<141>  2018-02-15

<150>  US62/459721
<151>  2017-02-16

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  542
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EAAT1T1

<400>  1

Met Thr Lys Ser Asn Gly Glu Glu Pro Lys Met Gly Gly Arg Met Glu 
1               5                   10                  15      


Arg Phe Gln Gln Gly Val Ser Lys Arg Thr Leu Leu Ala Lys Lys Lys 
            20                  25                  30          


Val Gln Asn Ile Thr Lys Glu Asp Val Lys Ser Phe Leu Arg Arg Asn 
        35                  40                  45              


Ala Leu Leu Leu Leu Thr Val Leu Ala Val Ile Leu Gly Val Val Leu 
    50                  55                  60                  


Gly Phe Leu Leu Arg Pro Tyr Pro Leu Ser Pro Arg Glu Val Lys Tyr 
65                  70                  75                  80  


Phe Ala Phe Pro Gly Glu Leu Leu Met Arg Met Leu Lys Met Leu Ile 
                85                  90                  95      


Leu Pro Leu Ile Val Ser Ser Leu Ile Thr Gly Leu Ala Ser Leu Asp 
            100                 105                 110         


Ala Lys Ala Ser Gly Arg Leu Gly Met Arg Ala Val Val Tyr Tyr Met 
        115                 120                 125             


Ser Thr Thr Ile Ile Ala Val Val Leu Gly Ile Ile Leu Val Leu Ile 
    130                 135                 140                 


Ile His Pro Gly Lys Gly Thr Lys Glu Asn Met His Arg Glu Gly Lys 
145                 150                 155                 160 


Ile Val Arg Val Thr Ala Ala Asp Ala Phe Leu Asp Leu Ile Arg Asn 
                165                 170                 175     


Met Phe Pro Glu Asn Leu Val Glu Ala Cys Phe Gln Gln Tyr Lys Thr 
            180                 185                 190         


Val Tyr Glu Lys Arg Ser Phe Lys Val Pro Ile Gln Ala Asn Glu Thr 
        195                 200                 205             


Leu Val Gly Ala Val Ile Asn Asn Val Ser Glu Ala Met Glu Thr Leu 
    210                 215                 220                 


Thr Arg Ile Thr Glu Glu Leu Val Pro Val Pro Gly Ser Val Asp Gly 
225                 230                 235                 240 


Met Asn Val Leu Gly Leu Val Val Phe Ser Ile Val Phe Gly Ile Ala 
                245                 250                 255     


Leu Gly Lys Met Gly Glu Gln Gly Gln Leu Leu Val Asp Phe Phe Asn 
            260                 265                 270         


Ser Leu Asn Glu Ala Thr Met Lys Leu Val Ala Ile Ile Met Trp Tyr 
        275                 280                 285             


Ala Pro Leu Gly Ile Leu Phe Leu Ile Ala Gly Lys Ile Val Glu Met 
    290                 295                 300                 


Glu Asp Leu Glu Val Leu Gly Gly Gln Leu Gly Met Tyr Met Val Thr 
305                 310                 315                 320 


Val Ile Val Gly Leu Val Ile His Gly Leu Ile Val Leu Pro Leu Ile 
                325                 330                 335     


Tyr Phe Leu Ile Thr Arg Lys Asn Pro Phe Val Phe Ile Ala Gly Ile 
            340                 345                 350         


Leu Gln Ala Leu Ile Thr Ala Leu Gly Thr Ser Ser Ser Ser Ala Thr 
        355                 360                 365             


Leu Pro Ile Thr Phe Lys Cys Leu Glu Glu Asn Asn Gly Val Asp Lys 
    370                 375                 380                 


Arg Ile Thr Arg Phe Val Leu Pro Val Gly Ala Thr Ile Asn Met Asp 
385                 390                 395                 400 


Gly Thr Ala Leu Tyr Glu Ala Val Ala Ala Ile Phe Ile Ala Gln Val 
                405                 410                 415     


Asn Asn Tyr Glu Leu Asp Phe Gly Gln Ile Ile Thr Ile Ser Ile Thr 
            420                 425                 430         


Ala Thr Ala Ala Ser Ile Gly Ala Ala Gly Ile Pro Gln Ala Gly Leu 
        435                 440                 445             


Val Thr Met Val Ile Val Leu Thr Ala Val Gly Leu Pro Thr Asp Asp 
    450                 455                 460                 


Ile Thr Leu Ile Ile Ala Val Asp Trp Leu Leu Asp Arg Phe Arg Thr 
465                 470                 475                 480 


Met Val Asn Val Leu Gly Asp Ala Leu Gly Ala Gly Ile Val Glu His 
                485                 490                 495     


Leu Ser Arg Lys Glu Leu Glu Lys Gln Asp Ala Glu Leu Gly Asn Ser 
            500                 505                 510         


Val Ile Glu Glu Asn Glu Met Lys Lys Pro Tyr Gln Leu Ile Ala Gln 
        515                 520                 525             


Asp Asn Glu Thr Glu Lys Pro Ile Asp Ser Glu Thr Lys Met 
    530                 535                 540         


<210>  2
<211>  542
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EAAT1T2

<400>  2

Met Thr Lys Ser Asn Gly Glu Glu Pro Lys Met Gly Gly Arg Met Glu 
1               5                   10                  15      


Arg Phe Gln Gln Gly Val Ser Lys Arg Thr Leu Leu Ala Lys Lys Lys 
            20                  25                  30          


Val Gln Asn Ile Thr Lys Glu Asp Val Lys Ser Tyr Leu Phe Arg Asn 
        35                  40                  45              


Ala Phe Val Leu Leu Thr Val Thr Ala Val Ile Leu Gly Thr Ile Leu 
    50                  55                  60                  


Gly Phe Thr Leu Arg Pro Tyr Arg Met Ser Tyr Arg Glu Val Lys Tyr 
65                  70                  75                  80  


Phe Ser Phe Pro Gly Glu Leu Leu Met Arg Met Leu Lys Met Leu Val 
                85                  90                  95      


Leu Pro Leu Ile Val Ser Ser Leu Ile Thr Gly Leu Ala Ala Leu Asp 
            100                 105                 110         


Ser Lys Ala Ser Gly Lys Met Gly Met Arg Ala Val Val Tyr Tyr Met 
        115                 120                 125             


Thr Thr Thr Ile Ile Ala Val Val Leu Gly Ile Ile Leu Val Leu Ile 
    130                 135                 140                 


Ile His Pro Gly Lys Ala Thr Lys Glu Asn Met His Arg Glu Gly Lys 
145                 150                 155                 160 


Ile Val Arg Val Thr Ala Ala Asp Ala Phe Leu Asp Leu Ile Arg Asn 
                165                 170                 175     


Met Phe Pro Glu Asn Leu Val Glu Ala Cys Phe Lys Gln Phe Lys Thr 
            180                 185                 190         


Asn Tyr Glu Lys Arg Ser Phe Lys Val Pro Ile Gln Ala Asn Glu Thr 
        195                 200                 205             


Leu Val Gly Ala Val Ile Asn Asn Val Ser Glu Ala Met Glu Thr Leu 
    210                 215                 220                 


Thr Arg Ile Thr Glu Glu Leu Val Pro Val Pro Gly Ser Val Asn Gly 
225                 230                 235                 240 


Val Asn Val Leu Gly Leu Val Val Phe Ser Ile Val Phe Gly Ile Ala 
                245                 250                 255     


Leu Gly Lys Met Gly Glu Gln Gly Gln Ala Leu Arg Glu Phe Phe Asp 
            260                 265                 270         


Ser Leu Asn Glu Ala Ile Met Lys Leu Val Ala Val Ile Met Trp Tyr 
        275                 280                 285             


Ala Pro Val Gly Ile Leu Phe Leu Ile Ala Gly Lys Ile Val Glu Met 
    290                 295                 300                 


Glu Asp Met Gly Val Ile Gly Gly Gln Leu Ala Met Tyr Met Val Thr 
305                 310                 315                 320 


Val Ile Val Gly Leu Leu Ile His Ala Val Ile Val Leu Pro Leu Ile 
                325                 330                 335     


Tyr Phe Leu Val Thr Arg Lys Asn Pro Trp Val Phe Ile Gly Gly Ile 
            340                 345                 350         


Leu Gln Ala Leu Ile Thr Ala Leu Gly Thr Ser Ser Ser Ser Ala Thr 
        355                 360                 365             


Leu Pro Ile Thr Phe Lys Cys Leu Glu Glu Asn Asn Gly Val Asp Lys 
    370                 375                 380                 


Arg Ile Thr Arg Phe Val Leu Pro Val Gly Ala Thr Ile Asn Met Asp 
385                 390                 395                 400 


Gly Thr Ala Leu Tyr Glu Ala Val Ala Ala Ile Phe Ile Ala Gln Val 
                405                 410                 415     


Asn Asn Phe Glu Leu Asn Phe Gly Gln Ile Ile Thr Ile Ser Ile Thr 
            420                 425                 430         


Ala Thr Ala Ala Ser Ile Gly Ala Ala Gly Ile Pro Gln Ala Gly Leu 
        435                 440                 445             


Val Thr Met Val Ile Val Leu Thr Ala Val Gly Leu Pro Thr Asp Asp 
    450                 455                 460                 


Ile Thr Leu Ile Ile Ala Val Asp Trp Phe Leu Asp Arg Phe Arg Thr 
465                 470                 475                 480 


Met Val Asn Val Leu Gly Asp Ala Leu Gly Ala Gly Ile Val Glu His 
                485                 490                 495     


Leu Ser Arg His Glu Leu Lys Asn Arg Asp Val Glu Met Gly Asn Ser 
            500                 505                 510         


Val Ile Glu Glu Asn Glu Met Lys Lys Pro Tyr Gln Leu Ile Ala Gln 
        515                 520                 525             


Asp Asn Glu Thr Glu Lys Pro Ile Asp Ser Glu Thr Lys Met 
    530                 535                 540         


<210>  3
<211>  522
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EAAT1cryst

<400>  3

Met Thr Lys Ser Asn Gly Glu Glu Pro Lys Met Gly Gly Arg Met Glu 
1               5                   10                  15      


Arg Phe Gln Gln Gly Val Ser Lys Arg Thr Leu Leu Ala Lys Lys Lys 
            20                  25                  30          


Val Gln Asn Ile Thr Lys Glu Asp Val Lys Ser Phe Leu Arg Arg Asn 
        35                  40                  45              


Ala Leu Leu Leu Leu Thr Val Leu Ala Val Ile Leu Gly Val Val Leu 
    50                  55                  60                  


Gly Phe Leu Leu Arg Pro Tyr Pro Leu Ser Pro Arg Glu Val Lys Tyr 
65                  70                  75                  80  


Phe Ala Phe Pro Gly Glu Leu Leu Met Arg Met Leu Lys Met Leu Ile 
                85                  90                  95      


Leu Pro Leu Ile Val Ser Ser Leu Ile Thr Gly Leu Ala Ser Leu Asp 
            100                 105                 110         


Ala Lys Ala Ser Gly Arg Leu Gly Met Arg Ala Val Val Tyr Tyr Met 
        115                 120                 125             


Ser Thr Thr Ile Ile Ala Val Val Leu Gly Ile Ile Leu Val Leu Ile 
    130                 135                 140                 


Ile His Pro Gly Ala Ala Ser Ala Ala Ile Thr Ala Ser Val Gly Ala 
145                 150                 155                 160 


Ala Gly Ser Ala Glu Asn Ala Pro Ser Lys Glu Val Leu Asp Ser Phe 
                165                 170                 175     


Leu Asp Leu Ala Arg Asn Ile Phe Pro Ser Asn Leu Val Ser Ala Ala 
            180                 185                 190         


Phe Arg Ser Tyr Ser Thr Thr Tyr Glu Glu Arg Thr Ile Thr Gly Thr 
        195                 200                 205             


Arg Val Lys Val Pro Val Gly Gln Glu Val Glu Gly Met Asn Ile Leu 
    210                 215                 220                 


Gly Leu Val Val Phe Ser Met Val Phe Gly Phe Ala Leu Gly Lys Met 
225                 230                 235                 240 


Gly Glu Gln Gly Gln Leu Leu Val Asp Phe Phe Asn Ser Leu Asn Glu 
                245                 250                 255     


Ala Thr Met Lys Leu Val Ala Ile Ile Met Trp Tyr Ala Pro Leu Gly 
            260                 265                 270         


Ile Leu Phe Leu Ile Ala Gly Lys Ile Val Glu Met Glu Asp Leu Glu 
        275                 280                 285             


Val Leu Gly Gly Gln Leu Gly Met Tyr Met Val Thr Val Ile Val Gly 
    290                 295                 300                 


Leu Val Ile His Gly Leu Ile Val Leu Pro Leu Ile Tyr Phe Leu Ile 
305                 310                 315                 320 


Thr Arg Lys Asn Pro Phe Val Phe Ile Ala Gly Ile Leu Gln Ala Leu 
                325                 330                 335     


Ile Thr Ala Leu Gly Thr Ser Ser Ser Ser Ala Thr Leu Pro Ile Thr 
            340                 345                 350         


Phe Lys Cys Leu Glu Glu Asn Asn Gly Val Asp Lys Arg Ile Thr Arg 
        355                 360                 365             


Phe Val Leu Pro Val Gly Ala Thr Ile Asn Met Asp Gly Thr Ala Leu 
    370                 375                 380                 


Tyr Glu Ala Val Ala Ala Ile Phe Ile Ala Gln Val Asn Asn Tyr Glu 
385                 390                 395                 400 


Leu Asp Phe Gly Gln Ile Ile Thr Ile Ser Ile Thr Ala Thr Ala Ala 
                405                 410                 415     


Ser Ile Gly Ala Ala Gly Ile Pro Gln Ala Gly Leu Val Thr Met Val 
            420                 425                 430         


Ile Val Leu Thr Ala Val Gly Leu Pro Thr Asp Asp Ile Thr Leu Ile 
        435                 440                 445             


Ile Ala Val Asp Trp Leu Leu Asp Arg Phe Arg Thr Met Val Asn Val 
    450                 455                 460                 


Leu Gly Asp Ala Leu Gly Ala Gly Ile Val Glu His Leu Ser Arg Lys 
465                 470                 475                 480 


Glu Leu Glu Lys Gln Asp Ala Glu Leu Gly Asn Ser Val Ile Glu Glu 
                485                 490                 495     


Asn Glu Met Lys Lys Pro Tyr Gln Leu Ile Ala Gln Asp Asn Glu Thr 
            500                 505                 510         


Glu Lys Pro Ile Asp Ser Glu Thr Lys Met 
        515                 520         


<210>  4
<211>  522
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EAAT1cryst-II

<400>  4

Met Thr Lys Ser Asn Gly Glu Glu Pro Lys Met Gly Gly Arg Met Glu 
1               5                   10                  15      


Arg Phe Gln Gln Gly Val Ser Lys Arg Thr Leu Leu Ala Lys Lys Lys 
            20                  25                  30          


Val Gln Asn Ile Thr Lys Glu Asp Val Lys Ser Phe Leu Arg Arg Asn 
        35                  40                  45              


Ala Leu Leu Leu Leu Thr Val Leu Ala Val Ile Leu Gly Val Val Leu 
    50                  55                  60                  


Gly Phe Leu Leu Arg Pro Tyr Pro Leu Ser Pro Arg Glu Val Lys Tyr 
65                  70                  75                  80  


Phe Ala Phe Pro Gly Glu Leu Leu Met Arg Met Leu Lys Met Leu Ile 
                85                  90                  95      


Leu Pro Leu Ile Val Ser Ser Leu Ile Thr Gly Leu Ala Ser Leu Asp 
            100                 105                 110         


Ala Lys Ala Ser Gly Arg Leu Gly Met Arg Ala Val Val Tyr Tyr Met 
        115                 120                 125             


Ser Thr Thr Ile Ile Ala Val Val Leu Gly Ile Ile Leu Val Leu Ile 
    130                 135                 140                 


Ile His Pro Gly Ala Ala Ser Ala Ala Ile Thr Ala Ser Val Gly Ala 
145                 150                 155                 160 


Ala Gly Ser Ala Glu Asn Ala Pro Ser Lys Glu Val Leu Asp Ser Phe 
                165                 170                 175     


Leu Asp Leu Ala Arg Asn Ile Phe Pro Ser Asn Leu Val Ser Ala Ala 
            180                 185                 190         


Phe Arg Ser Tyr Ser Thr Thr Tyr Glu Glu Arg Thr Ile Thr Gly Thr 
        195                 200                 205             


Arg Val Lys Val Pro Val Gly Gln Glu Val Glu Gly Met Asn Ile Leu 
    210                 215                 220                 


Gly Leu Val Val Phe Ser Ile Val Phe Gly Ile Ala Leu Gly Lys Met 
225                 230                 235                 240 


Gly Glu Gln Gly Gln Leu Leu Val Asp Phe Phe Asn Ser Leu Asn Glu 
                245                 250                 255     


Ala Thr Met Lys Leu Val Ala Ile Ile Met Trp Tyr Ala Pro Leu Gly 
            260                 265                 270         


Ile Leu Phe Leu Ile Ala Gly Lys Ile Val Glu Met Glu Asp Leu Glu 
        275                 280                 285             


Val Leu Gly Gly Gln Leu Gly Met Tyr Met Val Thr Val Ile Val Gly 
    290                 295                 300                 


Leu Val Ile His Gly Leu Ile Val Leu Pro Leu Ile Tyr Phe Leu Ile 
305                 310                 315                 320 


Thr Arg Lys Asn Pro Phe Val Phe Ile Ala Gly Ile Leu Gln Ala Leu 
                325                 330                 335     


Ile Thr Ala Leu Gly Thr Ser Ser Ser Ser Ala Thr Leu Pro Ile Thr 
            340                 345                 350         


Phe Lys Cys Leu Glu Glu Asn Asn Gly Val Asp Lys Arg Ile Thr Arg 
        355                 360                 365             


Phe Val Leu Pro Val Gly Ala Thr Ile Asn Met Asp Gly Thr Ala Leu 
    370                 375                 380                 


Tyr Glu Ala Val Ala Ala Ile Phe Ile Ala Gln Val Asn Asn Tyr Glu 
385                 390                 395                 400 


Leu Asp Phe Gly Gln Ile Ile Thr Ile Ser Ile Thr Ala Thr Ala Ala 
                405                 410                 415     


Ser Ile Gly Ala Ala Gly Ile Pro Gln Ala Gly Leu Val Thr Met Val 
            420                 425                 430         


Ile Val Leu Thr Ala Val Gly Leu Pro Thr Asp Asp Ile Thr Leu Ile 
        435                 440                 445             


Ile Ala Val Asp Trp Leu Leu Asp Arg Phe Arg Thr Met Val Asn Val 
    450                 455                 460                 


Leu Gly Asp Ala Leu Gly Ala Gly Ile Val Glu His Leu Ser Arg Lys 
465                 470                 475                 480 


Glu Leu Glu Lys Gln Asp Ala Glu Leu Gly Asn Ser Val Ile Glu Glu 
                485                 490                 495     


Asn Glu Met Lys Lys Pro Tyr Gln Leu Ile Ala Gln Asp Asn Glu Thr 
            500                 505                 510         


Glu Lys Pro Ile Asp Ser Glu Thr Lys Met 
        515                 520         


<210>  5
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EAAT2

<400>  5

Met Ala Ser Thr Glu Gly Ala Asn Asn Met Pro Lys Gln Val Glu Val 
1               5                   10                  15      


Arg Met His Asp Ser His Leu Gly Ser Glu Glu Pro Lys His Arg His 
            20                  25                  30          


Leu Gly Leu Arg Leu Cys Asp Lys Leu Gly Lys Asn Leu Leu Leu Thr 
        35                  40                  45              


Leu Thr Val Phe Gly Val Ile Leu Gly Ala Val Cys Gly Gly Leu Leu 
    50                  55                  60                  


Arg Leu Ala Ser Pro Ile His Pro Asp Val Val Met Leu Ile Ala Phe 
65                  70                  75                  80  


Pro Gly Asp Ile Leu Met Arg Met Leu Lys Met Leu Ile Leu Pro Leu 
                85                  90                  95      


Ile Ile Ser Ser Leu Ile Thr Gly Leu Ser Gly Leu Asp Ala Lys Ala 
            100                 105                 110         


Ser Gly Arg Leu Gly Thr Arg Ala Met Val Tyr Tyr Met Ser Thr Thr 
        115                 120                 125             


Ile Ile Ala Ala Val Leu Gly Val Ile Leu Val Leu Ala Ile His Pro 
    130                 135                 140                 


Gly Asn Pro Lys Leu Lys Lys Gln Leu Gly Pro Gly Lys Lys Asn Asp 
145                 150                 155                 160 


Glu Val Ser Ser Leu Asp Ala Phe Leu Asp Leu Ile Arg Asn Leu Phe 
                165                 170                 175     


Pro Glu Asn Leu Val Gln Ala Cys Phe Gln Gln Ile Gln Thr Val Thr 
            180                 185                 190         


Lys Lys Val Leu Val Ala Pro Pro Pro Asp Glu Glu Ala Asn Ala Thr 
        195                 200                 205             


Ser Ala Val Val Ser Leu Leu Asn Glu Thr Val Thr Glu Val Pro Glu 
    210                 215                 220                 


Glu Thr Lys Met Val Ile Lys Lys Gly Leu Glu Phe Lys Asp Gly Met 
225                 230                 235                 240 


Asn Val Leu Gly Leu Ile Gly Phe Phe Ile Ala Phe Gly Ile Ala Met 
                245                 250                 255     


Gly Lys Met Gly Asp Gln Ala Lys Leu Met Val Asp Phe Phe Asn Ile 
            260                 265                 270         


Leu Asn Glu Ile Val Met Lys Leu Val Ile Met Ile Met Trp Tyr Ser 
        275                 280                 285             


Pro Leu Gly Ile Ala Cys Leu Ile Cys Gly Lys Ile Ile Ala Ile Lys 
    290                 295                 300                 


Asp Leu Glu Val Val Ala Arg Gln Leu Gly Met Tyr Met Val Thr Val 
305                 310                 315                 320 


Ile Ile Gly Leu Ile Ile His Gly Gly Ile Phe Leu Pro Leu Ile Tyr 
                325                 330                 335     


Phe Val Val Thr Arg Lys Asn Pro Phe Ser Phe Phe Ala Gly Ile Phe 
            340                 345                 350         


Gln Ala Trp Ile Thr Ala Leu Gly Thr Ala Ser Ser Ala Gly Thr Leu 
        355                 360                 365             


Pro Val Thr Phe Arg Cys Leu Glu Glu Asn Leu Gly Ile Asp Lys Arg 
    370                 375                 380                 


Val Thr Arg Phe Val Leu Pro Val Gly Ala Thr Ile Asn Met Asp Gly 
385                 390                 395                 400 


Thr Ala Leu Tyr Glu Ala Val Ala Ala Ile Phe Ile Ala Gln Met Asn 
                405                 410                 415     


Gly Val Val Leu Asp Gly Gly Gln Ile Val Thr Val Ser Leu Thr Ala 
            420                 425                 430         


Thr Leu Ala Ser Val Gly Ala Ala Ser Ile Pro Ser Ala Gly Leu Val 
        435                 440                 445             


Thr Met Leu Leu Ile Leu Thr Ala Val Gly Leu Pro Thr Glu Asp Ile 
    450                 455                 460                 


Ser Leu Leu Val Ala Val Asp Trp Leu Leu Asp Arg Met Arg Thr Ser 
465                 470                 475                 480 


Val Asn Val Val Gly Asp Ser Phe Gly Ala Gly Ile Val Tyr His Leu 
                485                 490                 495     


Ser Lys Ser Glu Leu Asp Thr Ile Asp Ser Gln His Arg Val His Glu 
            500                 505                 510         


Asp Ile Glu Met Thr Lys Thr Gln Ser Ile Tyr Asp Asp Met Lys Asn 
        515                 520                 525             


His Arg Glu Ser Asn Ser Asn Gln Cys Val Tyr Ala Ala His Asn Ser 
    530                 535                 540                 


Val Ile Val Asp Glu Cys Lys Val Thr Leu Ala Ala Asn Gly Lys Ser 
545                 550                 555                 560 


Ala Asp Cys Ser Val Glu Glu Glu Pro Trp Lys Arg Glu Lys 
                565                 570                 


<210>  6
<211>  524
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EAAT3

<400>  6

Met Gly Lys Pro Ala Arg Lys Gly Cys Glu Trp Lys Arg Phe Leu Lys 
1               5                   10                  15      


Asn Asn Trp Val Leu Leu Ser Thr Val Ala Ala Val Val Leu Gly Ile 
            20                  25                  30          


Thr Thr Gly Val Leu Val Arg Glu His Ser Asn Leu Ser Thr Leu Glu 
        35                  40                  45              


Lys Phe Tyr Phe Ala Phe Pro Gly Glu Ile Leu Met Arg Met Leu Lys 
    50                  55                  60                  


Leu Ile Ile Leu Pro Leu Ile Ile Ser Ser Met Ile Thr Gly Val Ala 
65                  70                  75                  80  


Ala Leu Asp Ser Asn Val Ser Gly Lys Ile Gly Leu Arg Ala Val Val 
                85                  90                  95      


Tyr Tyr Phe Cys Thr Thr Leu Ile Ala Val Ile Leu Gly Ile Val Leu 
            100                 105                 110         


Val Val Ser Ile Lys Pro Gly Val Thr Gln Lys Val Gly Glu Ile Ala 
        115                 120                 125             


Arg Thr Gly Ser Thr Pro Glu Val Ser Thr Val Asp Ala Met Leu Asp 
    130                 135                 140                 


Leu Ile Arg Asn Met Phe Pro Glu Asn Leu Val Gln Ala Cys Phe Gln 
145                 150                 155                 160 


Gln Tyr Lys Thr Lys Arg Glu Glu Val Lys Pro Pro Ser Asp Pro Glu 
                165                 170                 175     


Met Asn Met Thr Glu Glu Ser Phe Thr Ala Val Met Thr Thr Ala Ile 
            180                 185                 190         


Ser Lys Asn Lys Thr Lys Glu Tyr Lys Ile Val Gly Met Tyr Ser Asp 
        195                 200                 205             


Gly Ile Asn Val Leu Gly Leu Ile Val Phe Cys Leu Val Phe Gly Leu 
    210                 215                 220                 


Val Ile Gly Lys Met Gly Glu Lys Gly Gln Ile Leu Val Asp Phe Phe 
225                 230                 235                 240 


Asn Ala Leu Ser Asp Ala Thr Met Lys Ile Val Gln Ile Ile Met Cys 
                245                 250                 255     


Tyr Met Pro Leu Gly Ile Leu Phe Leu Ile Ala Gly Lys Ile Ile Glu 
            260                 265                 270         


Val Glu Asp Trp Glu Ile Phe Arg Lys Leu Gly Leu Tyr Met Ala Thr 
        275                 280                 285             


Val Leu Thr Gly Leu Ala Ile His Ser Ile Val Ile Leu Pro Leu Ile 
    290                 295                 300                 


Tyr Phe Ile Val Val Arg Lys Asn Pro Phe Arg Phe Ala Met Gly Met 
305                 310                 315                 320 


Ala Gln Ala Leu Leu Thr Ala Leu Met Ile Ser Ser Ser Ser Ala Thr 
                325                 330                 335     


Leu Pro Val Thr Phe Arg Cys Ala Glu Glu Asn Asn Gln Val Asp Lys 
            340                 345                 350         


Arg Ile Thr Arg Phe Val Leu Pro Val Gly Ala Thr Ile Asn Met Asp 
        355                 360                 365             


Gly Thr Ala Leu Tyr Glu Ala Val Ala Ala Val Phe Ile Ala Gln Leu 
    370                 375                 380                 


Asn Asp Leu Asp Leu Gly Ile Gly Gln Ile Ile Thr Ile Ser Ile Thr 
385                 390                 395                 400 


Ala Thr Ser Ala Ser Ile Gly Ala Ala Gly Val Pro Gln Ala Gly Leu 
                405                 410                 415     


Val Thr Met Val Ile Val Leu Ser Ala Val Gly Leu Pro Ala Glu Asp 
            420                 425                 430         


Val Thr Leu Ile Ile Ala Val Asp Trp Leu Leu Asp Arg Phe Arg Thr 
        435                 440                 445             


Met Val Asn Val Leu Gly Asp Ala Phe Gly Thr Gly Ile Val Glu Lys 
    450                 455                 460                 


Leu Ser Lys Lys Glu Leu Glu Gln Met Asp Val Ser Ser Glu Val Asn 
465                 470                 475                 480 


Ile Val Asn Pro Phe Ala Leu Glu Ser Thr Ile Leu Asp Asn Glu Asp 
                485                 490                 495     


Ser Asp Thr Lys Lys Ser Tyr Val Asn Gly Gly Phe Ala Val Asp Lys 
            500                 505                 510         


Ser Asp Thr Ile Ser Phe Thr Gln Thr Ser Gln Phe 
        515                 520                 


<210>  7
<211>  564
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EAAT4

<400>  7

Met Ser Ser His Gly Asn Ser Leu Phe Leu Arg Glu Ser Gly Gln Arg 
1               5                   10                  15      


Leu Gly Arg Val Gly Trp Leu Gln Arg Leu Gln Glu Ser Leu Gln Gln 
            20                  25                  30          


Arg Ala Leu Arg Thr Arg Leu Arg Leu Gln Thr Met Thr Leu Glu His 
        35                  40                  45              


Val Leu Arg Phe Leu Arg Arg Asn Ala Phe Ile Leu Leu Thr Val Ser 
    50                  55                  60                  


Ala Val Val Ile Gly Val Ser Leu Ala Phe Ala Leu Arg Pro Tyr Gln 
65                  70                  75                  80  


Leu Thr Tyr Arg Gln Ile Lys Tyr Phe Ser Phe Pro Gly Glu Leu Leu 
                85                  90                  95      


Met Arg Met Leu Gln Met Leu Val Leu Pro Leu Ile Val Ser Ser Leu 
            100                 105                 110         


Val Thr Gly Met Ala Ser Leu Asp Asn Lys Ala Thr Gly Arg Met Gly 
        115                 120                 125             


Met Arg Ala Ala Val Tyr Tyr Met Val Thr Thr Ile Ile Ala Val Phe 
    130                 135                 140                 


Ile Gly Ile Leu Met Val Thr Ile Ile His Pro Gly Lys Gly Ser Lys 
145                 150                 155                 160 


Glu Gly Leu His Arg Glu Gly Arg Ile Glu Thr Ile Pro Thr Ala Asp 
                165                 170                 175     


Ala Phe Met Asp Leu Ile Arg Asn Met Phe Pro Pro Asn Leu Val Glu 
            180                 185                 190         


Ala Cys Phe Lys Gln Phe Lys Thr Gln Tyr Ser Thr Arg Val Val Thr 
        195                 200                 205             


Arg Thr Met Val Arg Thr Glu Asn Gly Ser Glu Pro Gly Ala Ser Met 
    210                 215                 220                 


Pro Pro Pro Phe Ser Val Glu Asn Gly Thr Ser Phe Leu Glu Asn Val 
225                 230                 235                 240 


Thr Arg Ala Leu Gly Thr Leu Gln Glu Met Leu Ser Phe Glu Glu Thr 
                245                 250                 255     


Val Pro Val Pro Gly Ser Ala Asn Gly Ile Asn Ala Leu Gly Leu Val 
            260                 265                 270         


Val Phe Ser Val Ala Phe Gly Leu Val Ile Gly Gly Met Lys His Lys 
        275                 280                 285             


Gly Arg Val Leu Arg Asp Phe Phe Asp Ser Leu Asn Glu Ala Ile Met 
    290                 295                 300                 


Arg Leu Val Gly Ile Ile Ile Trp Tyr Ala Pro Val Gly Ile Leu Phe 
305                 310                 315                 320 


Leu Ile Ala Gly Lys Ile Leu Glu Met Glu Asp Met Ala Val Leu Gly 
                325                 330                 335     


Gly Gln Leu Gly Met Tyr Thr Leu Thr Val Ile Val Gly Leu Phe Leu 
            340                 345                 350         


His Ala Gly Ile Val Leu Pro Leu Ile Tyr Phe Leu Val Thr His Arg 
        355                 360                 365             


Asn Pro Phe Pro Phe Ile Gly Gly Met Leu Gln Ala Leu Ile Thr Ala 
    370                 375                 380                 


Met Gly Thr Ser Ser Ser Ser Ala Thr Leu Pro Ile Thr Phe Arg Cys 
385                 390                 395                 400 


Leu Glu Glu Gly Leu Gly Val Asp Arg Arg Ile Thr Arg Phe Val Leu 
                405                 410                 415     


Pro Val Gly Ala Thr Val Asn Met Asp Gly Thr Ala Leu Tyr Glu Ala 
            420                 425                 430         


Leu Ala Ala Ile Phe Ile Ala Gln Val Asn Asn Tyr Glu Leu Asn Leu 
        435                 440                 445             


Gly Gln Ile Thr Thr Ile Ser Ile Thr Ala Thr Ala Ala Ser Val Gly 
    450                 455                 460                 


Ala Ala Gly Ile Pro Gln Ala Gly Leu Val Thr Met Val Ile Val Leu 
465                 470                 475                 480 


Thr Ser Val Gly Leu Pro Thr Glu Asp Ile Thr Leu Ile Ile Ala Val 
                485                 490                 495     


Asp Trp Phe Leu Asp Arg Leu Arg Thr Met Thr Asn Val Leu Gly Asp 
            500                 505                 510         


Ser Ile Gly Ala Ala Val Ile Glu His Leu Ser Gln Arg Glu Leu Glu 
        515                 520                 525             


Leu Gln Glu Ala Glu Leu Thr Leu Pro Ser Leu Gly Lys Pro Tyr Lys 
    530                 535                 540                 


Ser Leu Met Ala Gln Glu Lys Gly Ala Ser Arg Gly Arg Gly Gly Asn 
545                 550                 555                 560 


Glu Ser Ala Met 
                


<210>  8
<211>  560
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EAAT5

<400>  8

Met Val Pro His Ala Ile Leu Ala Arg Gly Arg Asp Val Cys Arg Arg 
1               5                   10                  15      


Asn Gly Leu Leu Ile Leu Ser Val Leu Ser Val Ile Val Gly Cys Leu 
            20                  25                  30          


Leu Gly Phe Phe Leu Arg Thr Arg Arg Leu Ser Pro Gln Glu Ile Ser 
        35                  40                  45              


Tyr Phe Gln Phe Pro Gly Glu Leu Leu Met Arg Met Leu Lys Met Met 
    50                  55                  60                  


Ile Leu Pro Leu Val Val Ser Ser Leu Met Ser Gly Leu Ala Ser Leu 
65                  70                  75                  80  


Asp Ala Lys Thr Ser Ser Arg Leu Gly Val Leu Thr Val Ala Tyr Tyr 
                85                  90                  95      


Leu Trp Thr Thr Phe Met Ala Val Ile Val Gly Ile Phe Met Val Ser 
            100                 105                 110         


Ile Ile His Pro Gly Ser Ala Ala Gln Lys Glu Thr Thr Glu Gln Ser 
        115                 120                 125             


Gly Lys Pro Ile Met Ser Ser Ala Asp Ala Leu Leu Asp Leu Ile Arg 
    130                 135                 140                 


Asn Met Phe Pro Ala Asn Leu Val Glu Ala Thr Phe Lys Gln Tyr Arg 
145                 150                 155                 160 


Thr Lys Thr Thr Pro Val Val Lys Ser Pro Lys Val Ala Pro Glu Glu 
                165                 170                 175     


Ala Pro Pro Arg Arg Ile Leu Ile Tyr Gly Val Gln Glu Glu Asn Gly 
            180                 185                 190         


Ser His Val Gln Asn Phe Ala Leu Asp Leu Thr Pro Pro Pro Glu Val 
        195                 200                 205             


Val Tyr Lys Ser Glu Pro Gly Thr Ser Asp Gly Met Asn Val Leu Gly 
    210                 215                 220                 


Ile Val Phe Phe Ser Ala Thr Met Gly Ile Met Leu Gly Arg Met Gly 
225                 230                 235                 240 


Asp Ser Gly Ala Pro Leu Val Ser Phe Cys Gln Cys Leu Asn Glu Ser 
                245                 250                 255     


Val Met Lys Ile Val Ala Val Ala Val Trp Tyr Phe Pro Phe Gly Ile 
            260                 265                 270         


Val Phe Leu Ile Ala Gly Lys Ile Leu Glu Met Asp Asp Pro Arg Ala 
        275                 280                 285             


Val Gly Lys Lys Leu Gly Phe Tyr Ser Val Thr Val Val Cys Gly Leu 
    290                 295                 300                 


Val Leu His Gly Leu Phe Ile Leu Pro Leu Leu Tyr Phe Phe Ile Thr 
305                 310                 315                 320 


Lys Lys Asn Pro Ile Val Phe Ile Arg Gly Ile Leu Gln Ala Leu Leu 
                325                 330                 335     


Ile Ala Leu Ala Thr Ser Ser Ser Ser Ala Thr Leu Pro Ile Thr Phe 
            340                 345                 350         


Lys Cys Leu Leu Glu Asn Asn His Ile Asp Arg Arg Ile Ala Arg Phe 
        355                 360                 365             


Val Leu Pro Val Gly Ala Thr Ile Asn Met Asp Gly Thr Ala Leu Tyr 
    370                 375                 380                 


Glu Ala Val Ala Ala Ile Phe Ile Ala Gln Val Asn Asn Tyr Glu Leu 
385                 390                 395                 400 


Asp Phe Gly Gln Ile Ile Thr Ile Ser Ile Thr Ala Thr Ala Ala Ser 
                405                 410                 415     


Ile Gly Ala Ala Gly Ile Pro Gln Ala Gly Leu Val Thr Met Val Ile 
            420                 425                 430         


Val Leu Thr Ser Val Gly Leu Pro Thr Asp Asp Ile Thr Leu Ile Ile 
        435                 440                 445             


Ala Val Asp Trp Ala Leu Asp Arg Phe Arg Thr Met Ile Asn Val Leu 
    450                 455                 460                 


Gly Asp Ala Leu Ala Ala Gly Ile Met Ala His Ile Cys Arg Lys Asp 
465                 470                 475                 480 


Phe Ala Arg Asp Thr Gly Thr Glu Lys Leu Leu Pro Cys Glu Thr Lys 
                485                 490                 495     


Pro Val Ser Leu Gln Glu Ile Val Ala Ala Gln Gln Asn Gly Cys Val 
            500                 505                 510         


Lys Ser Val Ala Glu Ala Ser Glu Leu Thr Leu Gly Pro Thr Cys Pro 
        515                 520                 525             


His His Val Pro Val Gln Val Glu Arg Asp Glu Glu Leu Pro Ala Ala 
    530                 535                 540                 


Ser Leu Asn His Cys Thr Ile Gln Ile Ser Glu Leu Glu Thr Asn Val 
545                 550                 555                 560 


<210>  9
<211>  532
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ASCT1

<400>  9

Met Glu Lys Ser Asn Glu Thr Asn Gly Tyr Leu Asp Ser Ala Gln Ala 
1               5                   10                  15      


Gly Pro Ala Ala Gly Pro Gly Ala Pro Gly Thr Ala Ala Gly Arg Ala 
            20                  25                  30          


Arg Arg Cys Ala Gly Phe Leu Arg Arg Gln Ala Leu Val Leu Leu Thr 
        35                  40                  45              


Val Ser Gly Val Leu Ala Gly Ala Gly Leu Gly Ala Ala Leu Arg Gly 
    50                  55                  60                  


Leu Ser Leu Ser Arg Thr Gln Val Thr Tyr Leu Ala Phe Pro Gly Glu 
65                  70                  75                  80  


Met Leu Leu Arg Met Leu Arg Met Ile Ile Leu Pro Leu Val Val Cys 
                85                  90                  95      


Ser Leu Val Ser Gly Ala Ala Ser Leu Asp Ala Ser Cys Leu Gly Arg 
            100                 105                 110         


Leu Gly Gly Ile Ala Val Ala Tyr Phe Gly Leu Thr Thr Leu Ser Ala 
        115                 120                 125             


Ser Ala Leu Ala Val Ala Leu Ala Phe Ile Ile Lys Pro Gly Ser Gly 
    130                 135                 140                 


Ala Gln Thr Leu Gln Ser Ser Asp Leu Gly Leu Glu Asp Ser Gly Pro 
145                 150                 155                 160 


Pro Pro Val Pro Lys Glu Thr Val Asp Ser Phe Leu Asp Leu Ala Arg 
                165                 170                 175     


Asn Leu Phe Pro Ser Asn Leu Val Val Ala Ala Phe Arg Thr Tyr Ala 
            180                 185                 190         


Thr Asp Tyr Lys Val Val Thr Gln Asn Ser Ser Ser Gly Asn Val Thr 
        195                 200                 205             


His Glu Lys Ile Pro Ile Gly Thr Glu Ile Glu Gly Met Asn Ile Leu 
    210                 215                 220                 


Gly Leu Val Leu Phe Ala Leu Val Leu Gly Val Ala Leu Lys Lys Leu 
225                 230                 235                 240 


Gly Ser Glu Gly Glu Asp Leu Ile Arg Phe Phe Asn Ser Leu Asn Glu 
                245                 250                 255     


Ala Thr Met Val Leu Val Ser Trp Ile Met Trp Tyr Val Pro Val Gly 
            260                 265                 270         


Ile Met Phe Leu Val Gly Ser Lys Ile Val Glu Met Lys Asp Ile Ile 
        275                 280                 285             


Val Leu Val Thr Ser Leu Gly Lys Tyr Ile Phe Ala Ser Ile Leu Gly 
    290                 295                 300                 


His Val Ile His Gly Gly Ile Val Leu Pro Leu Ile Tyr Phe Val Phe 
305                 310                 315                 320 


Thr Arg Lys Asn Pro Phe Arg Phe Leu Leu Gly Leu Leu Ala Pro Phe 
                325                 330                 335     


Ala Thr Ala Phe Ala Thr Cys Ser Ser Ser Ala Thr Leu Pro Ser Met 
            340                 345                 350         


Met Lys Cys Ile Glu Glu Asn Asn Gly Val Asp Lys Arg Ile Ser Arg 
        355                 360                 365             


Phe Ile Leu Pro Ile Gly Ala Thr Val Asn Met Asp Gly Ala Ala Ile 
    370                 375                 380                 


Phe Gln Cys Val Ala Ala Val Phe Ile Ala Gln Leu Asn Asn Val Glu 
385                 390                 395                 400 


Leu Asn Ala Gly Gln Ile Phe Thr Ile Leu Val Thr Ala Thr Ala Ser 
                405                 410                 415     


Ser Val Gly Ala Ala Gly Val Pro Ala Gly Gly Val Leu Thr Ile Ala 
            420                 425                 430         


Ile Ile Leu Glu Ala Ile Gly Leu Pro Thr His Asp Leu Pro Leu Ile 
        435                 440                 445             


Leu Ala Val Asp Trp Ile Val Asp Arg Thr Thr Thr Val Val Asn Val 
    450                 455                 460                 


Glu Gly Asp Ala Leu Gly Ala Gly Ile Leu His His Leu Asn Gln Lys 
465                 470                 475                 480 


Ala Thr Lys Lys Gly Glu Gln Glu Leu Ala Glu Val Lys Val Glu Ala 
                485                 490                 495     


Ile Pro Asn Cys Lys Ser Glu Glu Glu Thr Ser Pro Leu Val Thr His 
            500                 505                 510         


Gln Asn Pro Ala Gly Pro Val Ala Ser Ala Pro Glu Leu Glu Ser Lys 
        515                 520                 525             


Glu Ser Val Leu 
    530         


<210>  10
<211>  541
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ASCT2

<400>  10

Met Val Ala Asp Pro Pro Arg Asp Ser Lys Gly Leu Ala Ala Ala Glu 
1               5                   10                  15      


Pro Thr Ala Asn Gly Gly Leu Ala Leu Ala Ser Ile Glu Asp Gln Gly 
            20                  25                  30          


Ala Ala Ala Gly Gly Tyr Cys Gly Ser Arg Asp Gln Val Arg Arg Cys 
        35                  40                  45              


Leu Arg Ala Asn Leu Leu Val Leu Leu Thr Val Val Ala Val Val Ala 
    50                  55                  60                  


Gly Val Ala Leu Gly Leu Gly Val Ser Gly Ala Gly Gly Ala Leu Ala 
65                  70                  75                  80  


Leu Gly Pro Glu Arg Leu Ser Ala Phe Val Phe Pro Gly Glu Leu Leu 
                85                  90                  95      


Leu Arg Leu Leu Arg Met Ile Ile Leu Pro Leu Val Val Cys Ser Leu 
            100                 105                 110         


Ile Gly Gly Ala Ala Ser Leu Asp Pro Gly Ala Leu Gly Arg Leu Gly 
        115                 120                 125             


Ala Trp Ala Leu Leu Phe Phe Leu Val Thr Thr Leu Leu Ala Ser Ala 
    130                 135                 140                 


Leu Gly Val Gly Leu Ala Leu Ala Leu Gln Pro Gly Ala Ala Ser Ala 
145                 150                 155                 160 


Ala Ile Asn Ala Ser Val Gly Ala Ala Gly Ser Ala Glu Asn Ala Pro 
                165                 170                 175     


Ser Lys Glu Val Leu Asp Ser Phe Leu Asp Leu Ala Arg Asn Ile Phe 
            180                 185                 190         


Pro Ser Asn Leu Val Ser Ala Ala Phe Arg Ser Tyr Ser Thr Thr Tyr 
        195                 200                 205             


Glu Glu Arg Asn Ile Thr Gly Thr Arg Val Lys Val Pro Val Gly Gln 
    210                 215                 220                 


Glu Val Glu Gly Met Asn Ile Leu Gly Leu Val Val Phe Ala Ile Val 
225                 230                 235                 240 


Phe Gly Val Ala Leu Arg Lys Leu Gly Pro Glu Gly Glu Leu Leu Ile 
                245                 250                 255     


Arg Phe Phe Asn Ser Phe Asn Glu Ala Thr Met Val Leu Val Ser Trp 
            260                 265                 270         


Ile Met Trp Tyr Ala Pro Val Gly Ile Met Phe Leu Val Ala Gly Lys 
        275                 280                 285             


Ile Val Glu Met Glu Asp Val Gly Leu Leu Phe Ala Arg Leu Gly Lys 
    290                 295                 300                 


Tyr Ile Leu Cys Cys Leu Leu Gly His Ala Ile His Gly Leu Leu Val 
305                 310                 315                 320 


Leu Pro Leu Ile Tyr Phe Leu Phe Thr Arg Lys Asn Pro Tyr Arg Phe 
                325                 330                 335     


Leu Trp Gly Ile Val Thr Pro Leu Ala Thr Ala Phe Gly Thr Ser Ser 
            340                 345                 350         


Ser Ser Ala Thr Leu Pro Leu Met Met Lys Cys Val Glu Glu Asn Asn 
        355                 360                 365             


Gly Val Ala Lys His Ile Ser Arg Phe Ile Leu Pro Ile Gly Ala Thr 
    370                 375                 380                 


Val Asn Met Asp Gly Ala Ala Leu Phe Gln Cys Val Ala Ala Val Phe 
385                 390                 395                 400 


Ile Ala Gln Leu Ser Gln Gln Ser Leu Asp Phe Val Lys Ile Ile Thr 
                405                 410                 415     


Ile Leu Val Thr Ala Thr Ala Ser Ser Val Gly Ala Ala Gly Ile Pro 
            420                 425                 430         


Ala Gly Gly Val Leu Thr Leu Ala Ile Ile Leu Glu Ala Val Asn Leu 
        435                 440                 445             


Pro Val Asp His Ile Ser Leu Ile Leu Ala Val Asp Trp Leu Val Asp 
    450                 455                 460                 


Arg Ser Cys Thr Val Leu Asn Val Glu Gly Asp Ala Leu Gly Ala Gly 
465                 470                 475                 480 


Leu Leu Gln Asn Tyr Val Asp Arg Thr Glu Ser Arg Ser Thr Glu Pro 
                485                 490                 495     


Glu Leu Ile Gln Val Lys Ser Glu Leu Pro Leu Asp Pro Leu Pro Val 
            500                 505                 510         


Pro Thr Glu Glu Gly Asn Pro Leu Leu Lys His Tyr Arg Gly Pro Ala 
        515                 520                 525             


Gly Asp Ala Thr Val Ala Ser Glu Lys Glu Ser Val Met 
    530                 535                 540     


<210>  11
<211>  542
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EAAT1WT

<400>  11

Met Thr Lys Ser Asn Gly Glu Glu Pro Lys Met Gly Gly Arg Met Glu 
1               5                   10                  15      


Arg Phe Gln Gln Gly Val Arg Lys Arg Thr Leu Leu Ala Lys Lys Lys 
            20                  25                  30          


Val Gln Asn Ile Thr Lys Glu Asp Val Lys Ser Tyr Leu Phe Arg Asn 
        35                  40                  45              


Ala Phe Val Leu Leu Thr Val Thr Ala Val Ile Val Gly Thr Ile Leu 
    50                  55                  60                  


Gly Phe Thr Leu Arg Pro Tyr Arg Met Ser Tyr Arg Glu Val Lys Tyr 
65                  70                  75                  80  


Phe Ser Phe Pro Gly Glu Leu Leu Met Arg Met Leu Gln Met Leu Val 
                85                  90                  95      


Leu Pro Leu Ile Ile Ser Ser Leu Val Thr Gly Met Ala Ala Leu Asp 
            100                 105                 110         


Ser Lys Ala Ser Gly Lys Met Gly Met Arg Ala Val Val Tyr Tyr Met 
        115                 120                 125             


Thr Thr Thr Ile Ile Ala Val Val Ile Gly Ile Ile Ile Val Ile Ile 
    130                 135                 140                 


Ile His Pro Gly Lys Gly Thr Lys Glu Asn Met His Arg Glu Gly Lys 
145                 150                 155                 160 


Ile Val Arg Val Thr Ala Ala Asp Ala Phe Leu Asp Leu Ile Arg Asn 
                165                 170                 175     


Met Phe Pro Pro Asn Leu Val Glu Ala Cys Phe Lys Gln Phe Lys Thr 
            180                 185                 190         


Asn Tyr Glu Lys Arg Ser Phe Lys Val Pro Ile Gln Ala Asn Glu Thr 
        195                 200                 205             


Leu Val Gly Ala Val Ile Asn Asn Val Ser Glu Ala Met Glu Thr Leu 
    210                 215                 220                 


Thr Arg Ile Thr Glu Glu Leu Val Pro Val Pro Gly Ser Val Asn Gly 
225                 230                 235                 240 


Val Asn Ala Leu Gly Leu Val Val Phe Ser Met Cys Phe Gly Phe Val 
                245                 250                 255     


Ile Gly Asn Met Lys Glu Gln Gly Gln Ala Leu Arg Glu Phe Phe Asp 
            260                 265                 270         


Ser Leu Asn Glu Ala Ile Met Arg Leu Val Ala Val Ile Met Trp Tyr 
        275                 280                 285             


Ala Pro Val Gly Ile Leu Phe Leu Ile Ala Gly Lys Ile Val Glu Met 
    290                 295                 300                 


Glu Asp Met Gly Val Ile Gly Gly Gln Leu Ala Met Tyr Thr Val Thr 
305                 310                 315                 320 


Val Ile Val Gly Leu Leu Ile His Ala Val Ile Val Leu Pro Leu Leu 
                325                 330                 335     


Tyr Phe Leu Val Thr Arg Lys Asn Pro Trp Val Phe Ile Gly Gly Leu 
            340                 345                 350         


Leu Gln Ala Leu Ile Thr Ala Leu Gly Thr Ser Ser Ser Ser Ala Thr 
        355                 360                 365             


Leu Pro Ile Thr Phe Lys Cys Leu Glu Glu Asn Asn Gly Val Asp Lys 
    370                 375                 380                 


Arg Val Thr Arg Phe Val Leu Pro Val Gly Ala Thr Ile Asn Met Asp 
385                 390                 395                 400 


Gly Thr Ala Leu Tyr Glu Ala Leu Ala Ala Ile Phe Ile Ala Gln Val 
                405                 410                 415     


Asn Asn Phe Glu Leu Asn Phe Gly Gln Ile Ile Thr Ile Ser Ile Thr 
            420                 425                 430         


Ala Thr Ala Ala Ser Ile Gly Ala Ala Gly Ile Pro Gln Ala Gly Leu 
        435                 440                 445             


Val Thr Met Val Ile Val Leu Thr Ser Val Gly Leu Pro Thr Asp Asp 
    450                 455                 460                 


Ile Thr Leu Ile Ile Ala Val Asp Trp Phe Leu Asp Arg Leu Arg Thr 
465                 470                 475                 480 


Thr Thr Asn Val Leu Gly Asp Ser Leu Gly Ala Gly Ile Val Glu His 
                485                 490                 495     


Leu Ser Arg His Glu Leu Lys Asn Arg Asp Val Glu Met Gly Asn Ser 
            500                 505                 510         


Val Ile Glu Glu Asn Glu Met Lys Lys Pro Tyr Gln Leu Ile Ala Gln 
        515                 520                 525             


Asp Asn Glu Thr Glu Lys Pro Ile Asp Ser Glu Thr Lys Met 
    530                 535                 540         


<210>  12
<211>  1359
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NPC1L1WT

<400>  12

Met Ala Glu Ala Gly Leu Arg Gly Trp Leu Leu Trp Ala Leu Leu Leu 
1               5                   10                  15      


Arg Leu Ala Gln Ser Glu Pro Tyr Thr Thr Ile His Gln Pro Gly Tyr 
            20                  25                  30          


Cys Ala Phe Tyr Asp Glu Cys Gly Lys Asn Pro Glu Leu Ser Gly Ser 
        35                  40                  45              


Leu Met Thr Leu Ser Asn Val Ser Cys Leu Ser Asn Thr Pro Ala Arg 
    50                  55                  60                  


Lys Ile Thr Gly Asp His Leu Ile Leu Leu Gln Lys Ile Cys Pro Arg 
65                  70                  75                  80  


Leu Tyr Thr Gly Pro Asn Thr Gln Ala Cys Cys Ser Ala Lys Gln Leu 
                85                  90                  95      


Val Ser Leu Glu Ala Ser Leu Ser Ile Thr Lys Ala Leu Leu Thr Arg 
            100                 105                 110         


Cys Pro Ala Cys Ser Asp Asn Phe Val Asn Leu His Cys His Asn Thr 
        115                 120                 125             


Cys Ser Pro Asn Gln Ser Leu Phe Ile Asn Val Thr Arg Val Ala Gln 
    130                 135                 140                 


Leu Gly Ala Gly Gln Leu Pro Ala Val Val Ala Tyr Glu Ala Phe Tyr 
145                 150                 155                 160 


Gln His Ser Phe Ala Glu Gln Ser Tyr Asp Ser Cys Ser Arg Val Arg 
                165                 170                 175     


Val Pro Ala Ala Ala Thr Leu Ala Val Gly Thr Met Cys Gly Val Tyr 
            180                 185                 190         


Gly Ser Ala Leu Cys Asn Ala Gln Arg Trp Leu Asn Phe Gln Gly Asp 
        195                 200                 205             


Thr Gly Asn Gly Leu Ala Pro Leu Asp Ile Thr Phe His Leu Leu Glu 
    210                 215                 220                 


Pro Gly Gln Ala Val Gly Ser Gly Ile Gln Pro Leu Asn Glu Gly Val 
225                 230                 235                 240 


Ala Arg Cys Asn Glu Ser Gln Gly Asp Asp Val Ala Thr Cys Ser Cys 
                245                 250                 255     


Gln Asp Cys Ala Ala Ser Cys Pro Ala Ile Ala Arg Pro Gln Ala Leu 
            260                 265                 270         


Asp Ser Thr Phe Tyr Leu Gly Gln Met Pro Gly Ser Leu Val Leu Ile 
        275                 280                 285             


Ile Ile Leu Cys Ser Val Phe Ala Val Val Thr Ile Leu Leu Val Gly 
    290                 295                 300                 


Phe Arg Val Ala Pro Ala Arg Asp Lys Ser Lys Met Val Asp Pro Lys 
305                 310                 315                 320 


Lys Gly Thr Ser Leu Ser Asp Lys Leu Ser Phe Ser Thr His Thr Leu 
                325                 330                 335     


Leu Gly Gln Phe Phe Gln Gly Trp Gly Thr Trp Val Ala Ser Trp Pro 
            340                 345                 350         


Leu Thr Ile Leu Val Leu Ser Val Ile Pro Val Val Ala Leu Ala Ala 
        355                 360                 365             


Gly Leu Val Phe Thr Glu Leu Thr Thr Asp Pro Val Glu Leu Trp Ser 
    370                 375                 380                 


Ala Pro Asn Ser Gln Ala Arg Ser Glu Lys Ala Phe His Asp Gln His 
385                 390                 395                 400 


Phe Gly Pro Phe Phe Arg Thr Asn Gln Val Ile Leu Thr Ala Pro Asn 
                405                 410                 415     


Arg Ser Ser Tyr Arg Tyr Asp Ser Leu Leu Leu Gly Pro Lys Asn Phe 
            420                 425                 430         


Ser Gly Ile Leu Asp Leu Asp Leu Leu Leu Glu Leu Leu Glu Leu Gln 
        435                 440                 445             


Glu Arg Leu Arg His Leu Gln Val Trp Ser Pro Glu Ala Gln Arg Asn 
    450                 455                 460                 


Ile Ser Leu Gln Asp Ile Cys Tyr Ala Pro Leu Asn Pro Asp Asn Thr 
465                 470                 475                 480 


Ser Leu Tyr Asp Cys Cys Ile Asn Ser Leu Leu Gln Tyr Phe Gln Asn 
                485                 490                 495     


Asn Arg Thr Leu Leu Leu Leu Thr Ala Asn Gln Thr Leu Met Gly Gln 
            500                 505                 510         


Thr Ser Gln Val Asp Trp Lys Asp His Phe Leu Tyr Cys Ala Asn Ala 
        515                 520                 525             


Pro Leu Thr Phe Lys Asp Gly Thr Ala Leu Ala Leu Ser Cys Met Ala 
    530                 535                 540                 


Asp Tyr Gly Ala Pro Val Phe Pro Phe Leu Ala Ile Gly Gly Tyr Lys 
545                 550                 555                 560 


Gly Lys Asp Tyr Ser Glu Ala Glu Ala Leu Ile Met Thr Phe Ser Leu 
                565                 570                 575     


Asn Asn Tyr Pro Ala Gly Asp Pro Arg Leu Ala Gln Ala Lys Leu Trp 
            580                 585                 590         


Glu Glu Ala Phe Leu Glu Glu Met Arg Ala Phe Gln Arg Arg Met Ala 
        595                 600                 605             


Gly Met Phe Gln Val Thr Phe Met Ala Glu Arg Ser Leu Glu Asp Glu 
    610                 615                 620                 


Ile Asn Arg Thr Thr Ala Glu Asp Leu Pro Ile Phe Ala Thr Ser Tyr 
625                 630                 635                 640 


Ile Val Ile Phe Leu Tyr Ile Ser Leu Ala Leu Gly Ser Tyr Ser Ser 
                645                 650                 655     


Trp Ser Arg Val Met Val Asp Ser Lys Ala Thr Leu Gly Leu Gly Gly 
            660                 665                 670         


Val Ala Val Val Leu Gly Ala Val Met Ala Ala Met Gly Phe Phe Ser 
        675                 680                 685             


Tyr Leu Gly Ile Arg Ser Ser Leu Val Ile Leu Gln Val Val Pro Phe 
    690                 695                 700                 


Leu Val Leu Ser Val Gly Ala Asp Asn Ile Phe Ile Phe Val Leu Glu 
705                 710                 715                 720 


Tyr Gln Arg Leu Pro Arg Arg Pro Gly Glu Pro Arg Glu Val His Ile 
                725                 730                 735     


Gly Arg Ala Leu Gly Arg Val Ala Pro Ser Met Leu Leu Cys Ser Leu 
            740                 745                 750         


Ser Glu Ala Ile Cys Phe Phe Leu Gly Ala Leu Thr Pro Met Pro Ala 
        755                 760                 765             


Val Arg Thr Phe Ala Leu Thr Ser Gly Leu Ala Val Ile Leu Asp Phe 
    770                 775                 780                 


Leu Leu Gln Met Ser Ala Phe Val Ala Leu Leu Ser Leu Asp Ser Lys 
785                 790                 795                 800 


Arg Gln Glu Ala Ser Arg Leu Asp Val Cys Cys Cys Val Lys Pro Gln 
                805                 810                 815     


Glu Leu Pro Pro Pro Gly Gln Gly Glu Gly Leu Leu Leu Gly Phe Phe 
            820                 825                 830         


Gln Lys Ala Tyr Ala Pro Phe Leu Leu His Trp Ile Thr Arg Gly Val 
        835                 840                 845             


Val Leu Leu Leu Phe Leu Ala Leu Phe Gly Val Ser Leu Tyr Ser Met 
    850                 855                 860                 


Cys His Ile Ser Val Gly Leu Asp Gln Glu Leu Ala Leu Pro Lys Asp 
865                 870                 875                 880 


Ser Tyr Leu Leu Asp Tyr Phe Leu Phe Leu Asn Arg Tyr Phe Glu Val 
                885                 890                 895     


Gly Ala Pro Val Tyr Phe Val Thr Thr Leu Gly Tyr Asn Phe Ser Ser 
            900                 905                 910         


Glu Ala Gly Met Asn Ala Ile Cys Ser Ser Ala Gly Cys Asn Asn Phe 
        915                 920                 925             


Ser Phe Thr Gln Lys Ile Gln Tyr Ala Thr Glu Phe Pro Glu Gln Ser 
    930                 935                 940                 


Tyr Leu Ala Ile Pro Ala Ser Ser Trp Val Asp Asp Phe Ile Asp Trp 
945                 950                 955                 960 


Leu Thr Pro Ser Ser Cys Cys Arg Leu Tyr Ile Ser Gly Pro Asn Lys 
                965                 970                 975     


Asp Lys Phe Cys Pro Ser Thr Val Asn Ser Leu Asn Cys Leu Lys Asn 
            980                 985                 990         


Cys Met Ser Ile Thr Met Gly Ser  Val Arg Pro Ser Val  Glu Gln Phe 
        995                 1000                 1005             


His Lys  Tyr Leu Pro Trp Phe  Leu Asn Asp Arg Pro  Asn Ile Lys 
    1010                 1015                 1020             


Cys Pro  Lys Gly Gly Leu Ala  Ala Tyr Ser Thr Ser  Val Asn Leu 
    1025                 1030                 1035             


Thr Ser  Asp Gly Gln Val Leu  Asp Thr Val Ala Ile  Leu Ser Pro 
    1040                 1045                 1050             


Arg Leu  Glu Tyr Ser Gly Thr  Ile Ser Ala His Cys  Asn Leu Tyr 
    1055                 1060                 1065             


Leu Leu  Asp Ser Thr Ser Arg  Phe Met Ala Tyr His  Lys Pro Leu 
    1070                 1075                 1080             


Lys Asn  Ser Gln Asp Tyr Thr  Glu Ala Leu Arg Ala  Ala Arg Glu 
    1085                 1090                 1095             


Leu Ala  Ala Asn Ile Thr Ala  Asp Leu Arg Lys Val  Pro Gly Thr 
    1100                 1105                 1110             


Asp Pro  Ala Phe Glu Val Phe  Pro Tyr Thr Ile Thr  Asn Val Phe 
    1115                 1120                 1125             


Tyr Glu  Gln Tyr Leu Thr Ile  Leu Pro Glu Gly Leu  Phe Met Leu 
    1130                 1135                 1140             


Ser Leu  Cys Leu Val Pro Thr  Phe Ala Val Ser Cys  Leu Leu Leu 
    1145                 1150                 1155             


Gly Leu  Asp Leu Arg Ser Gly  Leu Leu Asn Leu Leu  Ser Ile Val 
    1160                 1165                 1170             


Met Ile  Leu Val Asp Thr Val  Gly Phe Met Ala Leu  Trp Gly Ile 
    1175                 1180                 1185             


Ser Tyr  Asn Ala Val Ser Leu  Ile Asn Leu Val Ser  Ala Val Gly 
    1190                 1195                 1200             


Met Ser  Val Glu Phe Val Ser  His Ile Thr Arg Ser  Phe Ala Ile 
    1205                 1210                 1215             


Ser Thr  Lys Pro Thr Trp Leu  Glu Arg Ala Lys Glu  Ala Thr Ile 
    1220                 1225                 1230             


Ser Met  Gly Ser Ala Val Phe  Ala Gly Val Ala Met  Thr Asn Leu 
    1235                 1240                 1245             


Pro Gly  Ile Leu Val Leu Gly  Leu Ala Lys Ala Gln  Leu Ile Gln 
    1250                 1255                 1260             


Ile Phe  Phe Phe Arg Leu Asn  Leu Leu Ile Thr Leu  Leu Gly Leu 
    1265                 1270                 1275             


Leu His  Gly Leu Val Phe Leu  Pro Val Ile Leu Ser  Tyr Val Gly 
    1280                 1285                 1290             


Pro Asp  Val Asn Pro Ala Leu  Ala Leu Glu Gln Lys  Arg Ala Glu 
    1295                 1300                 1305             


Glu Ala  Val Ala Ala Val Met  Val Ala Ser Cys Pro  Asn His Pro 
    1310                 1315                 1320             


Ser Arg  Val Ser Thr Ala Asp  Asn Ile Tyr Val Asn  His Ser Phe 
    1325                 1330                 1335             


Glu Gly  Ser Ile Lys Gly Ala  Gly Ala Ile Ser Asn  Phe Leu Pro 
    1340                 1345                 1350             


Asn Asn  Gly Arg Gln Phe 
    1355                 


<210>  13
<211>  1359
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NPC1L1T1

<400>  13

Met Ala Glu Ala Gly Leu Arg Gly Trp Leu Leu Trp Ala Leu Leu Leu 
1               5                   10                  15      


His Leu Ala Gln Ser Glu Pro Tyr Thr Pro Ile His Gln Ala Gly Tyr 
            20                  25                  30          


Cys Ala Phe Tyr Asp Glu Cys Gly Lys Asn Pro Glu Leu Ser Gly Ser 
        35                  40                  45              


Leu Ile Pro Leu Ser Asn Val Ser Cys Leu Ser Asn Thr Pro Ala Arg 
    50                  55                  60                  


Lys Val Thr Gly Asp His Leu Ile Leu Leu Gln Arg Ile Cys Pro Arg 
65                  70                  75                  80  


Leu Tyr Thr Gly Pro Thr Thr Tyr Ala Cys Cys Ser Leu Lys Gln Leu 
                85                  90                  95      


Val Ser Leu Glu Leu Ser Leu Ser Leu Ser Lys Ala Leu Leu Thr Arg 
            100                 105                 110         


Cys Pro Ala Cys Ala Glu Asn Phe Ala Asn Leu His Cys His Asn Thr 
        115                 120                 125             


Cys Ser Pro Asn Gln Ser Leu Phe Ile Asn Val Thr Arg Val Ala Gln 
    130                 135                 140                 


Leu Gly Ala Gly Gln Leu Pro Ala Val Val Ala Tyr Glu Ala Phe Tyr 
145                 150                 155                 160 


Gln Arg Ser Phe Ala Glu Gln Ala Tyr Asp Ser Cys Ser Arg Val Arg 
                165                 170                 175     


Ile Pro Ala Ala Ala Thr Leu Ala Val Gly Thr Met Cys Gly Val Tyr 
            180                 185                 190         


Gly Ser Ala Leu Cys Asn Ala Gln Arg Trp Leu Asn Phe Gln Gly Asp 
        195                 200                 205             


Thr Ser Asn Gly Leu Ala Pro Leu Asp Ile Thr Phe His Leu Leu Glu 
    210                 215                 220                 


Pro Gly Gln Ala Leu Gly Ser Gly Ile Gln Pro Leu Asn Gly Glu Val 
225                 230                 235                 240 


Trp Arg Cys Asn Glu Ser Gln Gly Asp Gly Ser Ala Ala Cys Ser Cys 
                245                 250                 255     


Gln Asp Cys Ala Ala Ser Cys Pro Val Ile Ala Gln Pro Pro Ala Leu 
            260                 265                 270         


Asp Ser Thr Phe Arg Leu Gly Gln Met Pro Gly Ser Leu Val Leu Ile 
        275                 280                 285             


Ile Ile Leu Cys Ser Val Phe Val Leu Leu Phe Ala Phe Leu Val Gly 
    290                 295                 300                 


Ser Arg Val Ala Ser Cys Arg Gly Lys Asp Lys Ala Lys Asp Pro Lys 
305                 310                 315                 320 


Lys Gly Thr Ser Cys Ser Asp Lys Leu Ser Leu Ser Thr His Thr Leu 
                325                 330                 335     


Leu Gly Arg Leu Phe Gln Ser Trp Gly Thr Trp Val Ala Ser Trp Pro 
            340                 345                 350         


Leu Thr Val Leu Ala Val Ser Val Ile Val Val Val Ala Leu Ala Gly 
        355                 360                 365             


Gly Leu Ala Phe Ile Glu Leu Thr Thr Asp Pro Val Glu Leu Trp Ser 
    370                 375                 380                 


Ala Pro Asn Ser Gln Ala Arg Gln Glu Lys Ala Phe His Asp Gln His 
385                 390                 395                 400 


Phe Gly Pro Phe Phe Arg Thr Asn Gln Val Ile Leu Thr Ala Pro Asn 
                405                 410                 415     


Arg Pro Ser Tyr Arg Tyr Asp Ser Leu Leu Leu Gly Pro Lys Asn Phe 
            420                 425                 430         


Ser Gly Ile Leu Ser Leu Asp Leu Leu Leu Glu Leu Leu Glu Leu Gln 
        435                 440                 445             


Glu Arg Leu Arg His Leu Gln Val Trp Ser Pro Glu Glu Gln Arg Asn 
    450                 455                 460                 


Ile Ser Leu Gln Asp Ile Cys Tyr Ala Pro Leu Asn Pro His Asn Ala 
465                 470                 475                 480 


Ser Leu Ser Asp Cys Cys Val Asn Ser Leu Leu Gln Tyr Phe Gln Asn 
                485                 490                 495     


Asn Arg Thr Asn Leu Leu Leu Thr Ala Asn Gln Thr Leu Met Gly Gln 
            500                 505                 510         


Thr Ser Gln Val Asp Trp Arg Asp His Phe Leu Tyr Cys Ala Asn Ser 
        515                 520                 525             


Pro Leu Thr Phe Lys Asp Gly Thr Ala Leu Ala Leu Ser Cys Met Ala 
    530                 535                 540                 


Asp Tyr Gly Ala Pro Val Phe Pro Phe Leu Ala Val Gly Gly Tyr Lys 
545                 550                 555                 560 


Gly Lys Asp Tyr Ser Glu Ala Glu Ala Leu Ile Met Thr Phe Ser Leu 
                565                 570                 575     


Asn Asn Tyr Pro Ala Gly Asp Pro Arg Leu Ala Gln Ala Lys Leu Trp 
            580                 585                 590         


Glu Glu Ala Phe Leu Glu Glu Met Arg Ala Phe Gln Arg Arg Thr Ala 
        595                 600                 605             


Gly Asn Phe Gln Val Thr Phe Met Ala Glu Arg Ser Leu Glu Asp Glu 
    610                 615                 620                 


Ile Asn Arg Thr Thr Ala Glu Asp Leu Pro Ile Phe Ala Ile Ser Tyr 
625                 630                 635                 640 


Leu Val Ile Phe Leu Tyr Ile Ser Leu Ala Leu Gly Ser Tyr Ser Ser 
                645                 650                 655     


Trp Ser Arg Val Leu Val Asp Ser Lys Ala Thr Leu Gly Leu Gly Gly 
            660                 665                 670         


Val Ala Val Val Leu Gly Ala Val Leu Ala Ser Met Gly Phe Phe Ser 
        675                 680                 685             


Tyr Leu Gly Val Pro Ser Ser Leu Val Ile Leu Gln Val Val Pro Phe 
    690                 695                 700                 


Leu Val Leu Ala Val Gly Ala Asp Asn Ile Phe Ile Phe Val Leu Glu 
705                 710                 715                 720 


Tyr Gln Arg Leu Pro Arg Arg Pro Gly Glu Pro Arg Glu Val His Ile 
                725                 730                 735     


Gly Arg Ala Leu Gly Arg Val Ala Pro Ser Met Leu Leu Cys Ser Leu 
            740                 745                 750         


Ser Glu Ala Ile Cys Phe Phe Leu Gly Ala Leu Thr Pro Met Pro Ala 
        755                 760                 765             


Val Arg Thr Phe Ala Leu Thr Ala Gly Leu Ala Val Ile Leu Asp Phe 
    770                 775                 780                 


Leu Leu Gln Met Ser Ala Phe Val Ala Leu Leu Ser Leu Asp Ser Arg 
785                 790                 795                 800 


Arg Gln Glu Ala Ser Arg Leu Asp Val Cys Cys Cys Val Lys Ala Gln 
                805                 810                 815     


Lys Leu Pro Pro Pro Lys Gln Gly Glu Gly Leu Leu Leu Arg Phe Phe 
            820                 825                 830         


Arg Lys Phe Tyr Ala Pro Phe Leu Leu His Arg Val Thr Arg Gly Val 
        835                 840                 845             


Val Leu Leu Leu Phe Leu Ala Leu Phe Gly Val Ser Leu Tyr Phe Met 
    850                 855                 860                 


Cys His Ile Ser Val Gly Leu Asp Gln Glu Leu Ala Leu Pro Lys Asp 
865                 870                 875                 880 


Ser Tyr Leu Leu Asp Tyr Phe Leu Phe Leu Asn Arg Tyr Phe Glu Val 
                885                 890                 895     


Gly Ala Pro Val Tyr Phe Val Thr Thr Gly Gly Tyr Asn Phe Ser Ser 
            900                 905                 910         


Glu Ala Gly Met Asn Ala Ile Cys Ser Ser Ala Gly Cys Asp Asn Phe 
        915                 920                 925             


Ser Leu Thr Gln Lys Ile Gln Tyr Ala Thr Glu Phe Pro Glu Gln Ser 
    930                 935                 940                 


Tyr Leu Ala Ile Pro Ala Ser Ser Trp Val Asp Asp Phe Ile Asp Trp 
945                 950                 955                 960 


Leu Thr Pro Ser Ser Cys Cys Arg Leu Tyr Ala Phe Gly Pro Asn Lys 
                965                 970                 975     


Asp Glu Phe Cys Pro Ser Thr Val Asn Ser Leu Asn Cys Leu Lys Lys 
            980                 985                 990         


Cys Met Ser Ile Thr Leu Gly Pro  Val Arg Pro Ser Val  Glu Gln Phe 
        995                 1000                 1005             


His Lys  Tyr Leu Pro Trp Phe  Leu Asn Asp Arg Pro  Asn Ile Lys 
    1010                 1015                 1020             


Cys Pro  Lys Gly Gly Leu Ala  Ala Tyr Asp Thr Ser  Val Asn Leu 
    1025                 1030                 1035             


Ser Ser  Asp Gly Gln Ile Leu  Asp Thr Val Ala Ile  Leu Ser Pro 
    1040                 1045                 1050             


Arg Leu  Glu Tyr Ser Gly Thr  Ile Ser Ala His Cys  Asn Leu Tyr 
    1055                 1060                 1065             


Leu Leu  Asp Ser Ala Ser Arg  Phe Met Ala Tyr His  Lys Pro Leu 
    1070                 1075                 1080             


Lys Asn  Ser Gln Asp Tyr Thr  Glu Ala Leu Arg Ala  Ala Arg Glu 
    1085                 1090                 1095             


Leu Ala  Ala Asn Ile Thr Ala  Asp Leu Arg Lys Val  Pro Gly Thr 
    1100                 1105                 1110             


Asp Pro  Ala Phe Glu Val Phe  Pro Tyr Thr Ile Thr  Asn Val Phe 
    1115                 1120                 1125             


Tyr Glu  Gln Tyr Leu Thr Ile  Val Pro Glu Gly Leu  Phe Met Leu 
    1130                 1135                 1140             


Ala Leu  Cys Leu Val Pro Thr  Phe Ala Val Cys Cys  Leu Leu Leu 
    1145                 1150                 1155             


Gly Met  Asp Leu Arg Ser Gly  Leu Leu Asn Leu Phe  Ser Ile Ile 
    1160                 1165                 1170             


Met Ile  Leu Val Asp Thr Val  Gly Phe Met Ala Leu  Trp Gly Ile 
    1175                 1180                 1185             


Ser Tyr  Asn Ala Val Ser Leu  Ile Asn Leu Val Thr  Ala Val Gly 
    1190                 1195                 1200             


Ile Ser  Val Glu Phe Val Ser  His Ile Thr Arg Ser  Phe Ala Ile 
    1205                 1210                 1215             


Ser Thr  Lys Pro Thr Arg Leu  Glu Arg Ala Lys Glu  Ala Thr Ile 
    1220                 1225                 1230             


Ser Met  Gly Ser Ala Val Phe  Ala Gly Val Ala Met  Thr Asn Leu 
    1235                 1240                 1245             


Pro Gly  Ile Leu Val Leu Gly  Leu Ala Lys Ala Gln  Leu Ile Gln 
    1250                 1255                 1260             


Ile Phe  Phe Phe Arg Leu Asn  Leu Leu Ile Thr Leu  Leu Gly Leu 
    1265                 1270                 1275             


Leu His  Gly Leu Val Phe Leu  Pro Val Ile Leu Ser  Tyr Leu Gly 
    1280                 1285                 1290             


Pro Asp  Val Asn Gln Ala Leu  Val Leu Glu Gln Lys  Arg Ala Glu 
    1295                 1300                 1305             


Glu Ala  Val Ala Ala Val Met  Glu Ala Ser Cys Pro  Asn His Pro 
    1310                 1315                 1320             


Ser Arg  Val Ser Thr Ala Asp  Asn Ile Tyr Val Asn  His Ser Phe 
    1325                 1330                 1335             


Glu His  Pro Ala Lys Gly Ala  Gly Ala Ile Ser Ser  Ser Leu Pro 
    1340                 1345                 1350             


Asn Asn  Gly Arg Gln Phe 
    1355                 


<210>  14
<211>  1325
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NPC1L1

<400>  14

Met Ala Asp Thr Gly Leu Arg Gly Trp Leu Leu Trp Ala Leu Leu Leu 
1               5                   10                  15      


His Val Ala Gln Ser Glu Leu Tyr Thr Pro Ile His Gln Pro Gly Tyr 
            20                  25                  30          


Cys Ala Phe Tyr Asp Glu Cys Gly Lys Asn Pro Glu Leu Ser Gly Gly 
        35                  40                  45              


Leu Ala Pro Leu Ser Asn Val Ser Cys Leu Ser Asn Thr Pro Ala Leu 
    50                  55                  60                  


Arg Val Thr Gly Glu His Leu Thr Leu Leu Gln Arg Ile Cys Pro Arg 
65                  70                  75                  80  


Leu Tyr Thr Gly Thr Thr Thr Tyr Ala Cys Cys Ser Pro Lys Gln Leu 
                85                  90                  95      


Leu Ser Leu Glu Thr Ser Leu Ala Val Thr Lys Ala Leu Leu Thr Arg 
            100                 105                 110         


Cys Pro Thr Cys Ser Asp Asn Phe Val Asn Leu His Cys Gln Asn Thr 
        115                 120                 125             


Cys Ser Pro Asn Gln Ser Leu Phe Ile Asn Val Thr Arg Val Ala Gly 
    130                 135                 140                 


Gly Gly Gly Gly Arg Pro Gln Ala Val Val Ala Tyr Glu Ala Phe Tyr 
145                 150                 155                 160 


Gln Asp Thr Phe Ala Gln Gln Thr Tyr Asp Ser Cys Ser Arg Val Arg 
                165                 170                 175     


Ile Pro Ala Ala Ala Thr Leu Ala Val Gly Thr Met Cys Gly Val Tyr 
            180                 185                 190         


Gly Ser Thr Leu Cys Asn Ala Gln Arg Trp Leu Asn Phe Gln Gly Asp 
        195                 200                 205             


Thr Ser Asn Gly Leu Ala Pro Leu Asp Ile Thr Phe His Leu Met Glu 
    210                 215                 220                 


Pro Gly Gln Ala Leu Gly Ser Gly Met Gln Ala Leu Thr Gly Glu Ile 
225                 230                 235                 240 


Arg Pro Cys Asn Glu Ser Gln Gly Asn Gly Thr Val Ala Cys Ser Cys 
                245                 250                 255     


Gln Asp Cys Ala Ala Ser Cys Pro Thr Ile Pro Gln Pro Gln Ala Leu 
            260                 265                 270         


Asp Ser Thr Phe Tyr Leu Gly Gly Leu Glu Gly Gly Leu Ala Leu Val 
        275                 280                 285             


Ile Ile Leu Cys Ser Ala Phe Ala Leu Leu Thr Thr Phe Leu Val Gly 
    290                 295                 300                 


Thr Arg Leu Ala Ser Ser Cys Gly Lys Asp Lys Thr Pro Asp Pro Lys 
305                 310                 315                 320 


Ala Gly Met Ser Leu Ser Asp Lys Leu Ser Leu Ser Thr Asn Val Ile 
                325                 330                 335     


Leu Ser Gln Cys Phe Gln Asn Trp Gly Thr Trp Val Ala Ser Trp Pro 
            340                 345                 350         


Leu Thr Ile Leu Leu Val Ser Ile Ala Val Val Leu Ala Leu Ser Gly 
        355                 360                 365             


Gly Leu Ala Phe Val Glu Leu Thr Thr Asp Pro Val Glu Leu Trp Ser 
    370                 375                 380                 


Ala Pro Ser Ser Gln Ala Arg Ser Glu Lys Ala Phe His Asp Gln His 
385                 390                 395                 400 


Phe Gly Pro Phe Leu Arg Thr Asn Gln Val Ile Leu Thr Ala Pro Asn 
                405                 410                 415     


Arg Pro Ser Tyr His Tyr Asp Ser Leu Leu Leu Gly Pro Lys Asn Phe 
            420                 425                 430         


Ser Gly Val Leu Ala Ser Asp Leu Leu Leu Glu Leu Leu Glu Leu Gln 
        435                 440                 445             


Glu Thr Leu Arg His Leu Gln Val Trp Ser Pro Glu Glu Gln Arg His 
    450                 455                 460                 


Ile Ser Leu Gln Asp Ile Cys Phe Ala Pro Leu Asn Pro His Asn Ala 
465                 470                 475                 480 


Ser Leu Ser Asp Cys Cys Ile Asn Ser Leu Leu Gln Tyr Phe Gln Ser 
                485                 490                 495     


Asn Arg Thr His Leu Leu Leu Thr Ala Asn Gln Thr Leu Thr Gly Gln 
            500                 505                 510         


Thr Ser Gln Val Asp Trp Arg Asp His Phe Leu Tyr Cys Ala Asn Ala 
        515                 520                 525             


Pro Leu Thr Phe Lys Asp Gly Thr Ala Leu Ala Leu Ser Cys Met Ala 
    530                 535                 540                 


Asp Tyr Gly Gly Pro Val Phe Pro Phe Leu Ala Val Gly Gly Tyr Lys 
545                 550                 555                 560 


Gly Lys Asp Tyr Ser Glu Ala Glu Ala Leu Ile Met Thr Phe Ser Leu 
                565                 570                 575     


Asn Asn Tyr Ala Pro Gly Asp Pro Arg Leu Ala Gln Ala Lys Leu Trp 
            580                 585                 590         


Glu Ala Ala Phe Leu Glu Glu Met Lys Ala Phe Gln Arg Arg Thr Ala 
        595                 600                 605             


Gly Thr Phe Gln Val Thr Phe Met Ala Glu Arg Ser Leu Glu Asp Glu 
    610                 615                 620                 


Ile Asn Arg Thr Thr Ala Glu Asp Leu Pro Ile Phe Gly Val Ser Tyr 
625                 630                 635                 640 


Ile Ile Ile Phe Leu Tyr Ile Ser Leu Ala Leu Gly Ser Tyr Ser Ser 
                645                 650                 655     


Trp Arg Arg Val Pro Val Asp Ser Lys Val Thr Leu Gly Leu Gly Gly 
            660                 665                 670         


Val Ala Val Val Leu Gly Ala Val Thr Ala Ala Met Gly Phe Phe Ser 
        675                 680                 685             


Tyr Leu Gly Val Pro Ser Ser Leu Val Ile Leu Gln Val Val Pro Phe 
    690                 695                 700                 


Leu Val Leu Ala Val Gly Ala Asp Asn Ile Phe Ile Phe Val Leu Glu 
705                 710                 715                 720 


Tyr Gln Arg Leu Pro Arg Arg Pro Gly Glu Pro Arg Glu Ala His Ile 
                725                 730                 735     


Gly Arg Ala Leu Gly Ser Val Ala Pro Ser Met Leu Leu Cys Ser Leu 
            740                 745                 750         


Ser Glu Ala Ile Cys Phe Phe Leu Gly Ala Leu Thr Pro Met Pro Ala 
        755                 760                 765             


Val Lys Thr Phe Ala Leu Ile Ser Gly Phe Ala Ile Val Leu Asp Phe 
    770                 775                 780                 


Leu Leu Gln Val Ser Ala Phe Val Ala Leu Leu Ser Leu Asp Ser Arg 
785                 790                 795                 800 


Arg Gln Glu Ala Ser Arg Leu Asp Val Cys Cys Cys Val Ser Ala Pro 
                805                 810                 815     


Lys Leu Pro Ala Pro Gly Gln Ser Glu Gly Leu Leu Leu Arg Val Phe 
            820                 825                 830         


Arg Lys Phe Tyr Val Pro Val Leu Leu His Arg Val Thr Arg Ala Val 
        835                 840                 845             


Val Leu Leu Leu Phe Thr Gly Leu Phe Gly Val Gly Leu Tyr Phe Met 
    850                 855                 860                 


Cys His Ile Arg Val Gly Leu Asp Gln Glu Leu Ala Leu Pro Lys Asp 
865                 870                 875                 880 


Ser Tyr Leu Leu Asp Tyr Phe Phe Phe Leu Asn Arg Tyr Phe Glu Val 
                885                 890                 895     


Gly Ala Pro Val Tyr Phe Val Thr Thr Gly Gly Tyr Asn Phe Ser Ser 
            900                 905                 910         


Glu Ala Gly Met Asn Ala Val Cys Ser Ser Ala Gly Cys Asp Ser Tyr 
        915                 920                 925             


Ser Leu Thr Gln Lys Ile Gln Tyr Ala Thr Glu Phe Pro Glu Glu Ser 
    930                 935                 940                 


Tyr Leu Ala Ile Pro Ala Ser Ser Trp Val Asp Asp Phe Ile Asp Trp 
945                 950                 955                 960 


Leu Thr Pro Ser Ser Cys Cys Arg Leu Tyr Ala Phe Gly Ala Asn Lys 
                965                 970                 975     


Asp Lys Phe Cys Pro Ser Thr Val Asn Ser Leu Ala Cys Leu Lys Asn 
            980                 985                 990         


Cys Val Asn Phe Thr Leu Gly Pro  Val Arg Pro Ser Val  Asp Gln Phe 
        995                 1000                 1005             


His Lys  Tyr Leu Pro Trp Phe  Leu Ser Asp Pro Pro  Asn Ile Lys 
    1010                 1015                 1020             


Cys Pro  Lys Gly Gly Leu Ala  Ala Tyr Asn Thr Ser  Val His Leu 
    1025                 1030                 1035             


Gly Ser  Asp Gly Gln Val Leu  Ala Ser Arg Phe Met  Ala Tyr His 
    1040                 1045                 1050             


Lys Pro  Leu Arg Asn Ser Glu  Asp Tyr Thr Glu Ala  Leu Arg Val 
    1055                 1060                 1065             


Ser Arg  Ala Leu Ala Ala Asn  Ile Thr Ala Gln Leu  Arg Gln Val 
    1070                 1075                 1080             


Pro Gly  Thr Asp Pro Ala Phe  Glu Val Phe Pro Tyr  Thr Ile Thr 
    1085                 1090                 1095             


Asn Val  Phe Tyr Glu Gln Tyr  Leu Ser Val Val Pro  Glu Gly Leu 
    1100                 1105                 1110             


Phe Met  Leu Ala Ile Cys Leu  Leu Pro Thr Phe Val  Val Cys Cys 
    1115                 1120                 1125             


Leu Leu  Leu Gly Met Asp Leu  Arg Ser Gly Leu Leu  Asn Leu Phe 
    1130                 1135                 1140             


Ser Ile  Val Met Ile Leu Val  Asp Thr Val Gly Phe  Met Ala Leu 
    1145                 1150                 1155             


Trp Gly  Ile Ser Tyr Asn Ala  Val Ser Leu Ile Asn  Leu Val Thr 
    1160                 1165                 1170             


Ala Val  Gly Ile Ser Val Glu  Phe Val Ser His Ile  Thr Arg Ser 
    1175                 1180                 1185             


Phe Ala  Val Ser Thr Arg Pro  Thr Arg Leu Glu Arg  Ala Lys Glu 
    1190                 1195                 1200             


Ala Thr  Ile Ser Met Gly Ser  Ala Val Phe Ala Gly  Val Ala Met 
    1205                 1210                 1215             


Thr Asn  Leu Pro Gly Ile Leu  Val Leu Gly Leu Ala  Lys Ala Gln 
    1220                 1225                 1230             


Leu Ile  Gln Ile Phe Phe Phe  Arg Leu Asn Leu Leu  Ile Thr Val 
    1235                 1240                 1245             


Leu Gly  Leu Leu His Gly Leu  Val Phe Leu Pro Val  Val Leu Ser 
    1250                 1255                 1260             


Tyr Leu  Gly Pro Asp Ile Asn  Ala Ala Leu Val Leu  Asp Gln Lys 
    1265                 1270                 1275             


Lys Thr  Glu Glu Ala Ile Gly  Ala Pro Ala His Leu  Val Pro Thr 
    1280                 1285                 1290             


Ser Thr  Ala Ser Ser Thr Tyr  Val Asn Tyr Gly Phe  Gln His Pro 
    1295                 1300                 1305             


Ala Asn  Gly Val Val Gly Asp  Ser Ser Leu Pro Arg  Ser Gly Pro 
    1310                 1315                 1320             


Asp Leu  
    1325 


<210>  15
<211>  1333
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NPC1L1

<400>  15

Met Val Glu Thr Gly Leu Lys Gly Trp Leu Leu Trp Ala Leu Leu Leu 
1               5                   10                  15      


His Ser Ala Gln Ser Glu Val Tyr Thr Pro Ile His Gln Pro Gly Tyr 
            20                  25                  30          


Cys Ala Phe Tyr Asp Glu Cys Gly Lys Asn Pro Glu Leu Ser Gly Ser 
        35                  40                  45              


Leu Ala Ser Leu Ser Asn Val Ser Cys Leu Asp Asn Ser Pro Ala Arg 
    50                  55                  60                  


His Ile Thr Gly Asp His Leu Ala Leu Leu Gln Ser Ile Cys Pro Arg 
65                  70                  75                  80  


Leu Tyr Thr Gly Ala Ser Thr Thr Tyr Ala Cys Cys Ser Ser Lys Gln 
                85                  90                  95      


Leu Val Ala Leu Asp Met Ser Leu Arg Ile Thr Lys Ala Leu Leu Thr 
            100                 105                 110         


Arg Cys Pro Ala Cys Ser Asp Asn Phe Val Ser Leu His Cys His Asn 
        115                 120                 125             


Thr Cys Ser Pro Asn Gln Ser Leu Phe Ile Asn Val Thr Arg Val Val 
    130                 135                 140                 


Thr Gln Gly Asp Ser Gln Ser Gln Ala Val Val Ala Tyr Glu Ala Phe 
145                 150                 155                 160 


Tyr Gln Arg Ser Phe Ala Glu Gln Thr Tyr Asn Ser Cys Ser Arg Val 
                165                 170                 175     


Arg Ile Pro Ala Ala Ala Thr Leu Ala Val Gly Ser Met Cys Gly Val 
            180                 185                 190         


Tyr Gly Ser Ala Leu Cys Asn Ala Gln Arg Trp Leu Asn Phe Gln Gly 
        195                 200                 205             


Asp Thr Ser Asn Gly Leu Ala Pro Leu Asp Ile Thr Phe His Leu Trp 
    210                 215                 220                 


Glu Pro Ser Gln Ala Glu Gly Ser Thr Ile Gln Pro Leu Asn Asp Glu 
225                 230                 235                 240 


Val Val Pro Cys Asn Gln Ser Gln Gly Asp Gly Ala Ala Ala Cys Ser 
                245                 250                 255     


Cys Gln Asp Cys Ala Ala Ser Cys Pro Val Ile Ala Gln Pro Arg Ala 
            260                 265                 270         


Leu Asp Pro Thr Phe Arg Leu Gly Arg Met Glu Gly Ser Leu Val Leu 
        275                 280                 285             


Ile Ile Ile Leu Cys Ser Leu Phe Val Leu Leu Thr Ala Phe Leu Leu 
    290                 295                 300                 


Arg Ser Arg Leu Ala Glu Trp Cys Arg Gly Lys Arg Lys Thr Pro Lys 
305                 310                 315                 320 


Pro Lys Ala Ser Ile Asn Leu Ala His Arg Leu Ser Leu Ser Thr His 
                325                 330                 335     


Thr Leu Leu Ser Arg Cys Phe Gln Cys Trp Gly Thr Trp Val Ala Ser 
            340                 345                 350         


Trp Pro Leu Thr Ile Leu Ala Val Ser Val Ile Val Val Val Ala Leu 
        355                 360                 365             


Ala Gly Gly Leu Ala Phe Ile Glu Leu Thr Thr Asp Pro Val Glu Leu 
    370                 375                 380                 


Trp Ser Ala Pro Asn Ser Leu Ala Arg Arg Glu Lys Ala Phe His Asp 
385                 390                 395                 400 


Lys Tyr Phe Gly Pro Phe Phe Arg Thr Ser Gln Val Phe Met Thr Ala 
                405                 410                 415     


Pro His Arg Pro Ser Tyr Arg Tyr Asp Ser Leu Leu Leu Gly Pro Lys 
            420                 425                 430         


Asn Phe Ser Gly Ile Leu Ser Ser Asp Leu Leu Leu Glu Val Leu Glu 
        435                 440                 445             


Leu Gln Glu Arg Leu Arg His Leu Gln Val Trp Ser Pro Glu Glu Gln 
    450                 455                 460                 


Arg Asn Val Ser Leu Arg Asp Thr Cys Tyr Ala Pro Leu Asn Pro His 
465                 470                 475                 480 


Asn Ala Ser Leu Ser Asp Cys Cys Val Asn Ser Leu Leu Gln Tyr Phe 
                485                 490                 495     


Gln Asn Asn Arg Thr Gln Leu Leu Leu Thr Ala Asn Gln Thr Leu Ser 
            500                 505                 510         


Gly Gln Thr Ala Gln Val Asp Trp Arg Asp His Phe Leu Tyr Cys Ala 
        515                 520                 525             


Asn Ala Pro Leu Thr Tyr Lys Asp Gly Thr Ala Leu Ala Leu Ser Cys 
    530                 535                 540                 


Met Ala Asp Tyr Gly Ala Pro Ile Phe Pro Phe Leu Ala Val Gly Gly 
545                 550                 555                 560 


Tyr Lys Gly Lys Asp Tyr Ser Glu Ala Glu Ala Leu Ile Met Thr Phe 
                565                 570                 575     


Ser Leu Asn Asn Tyr Pro Pro Gly Asp Pro Lys Leu Ala Gln Ala Lys 
            580                 585                 590         


Leu Trp Glu Ala Ala Phe Leu Glu Glu Met Arg Ala Phe Gln Arg Arg 
        595                 600                 605             


Thr Ala Gly Val Phe Gln Val Thr Phe Met Ala Glu Arg Ser Leu Glu 
    610                 615                 620                 


Asp Glu Ile Asn Ser Thr Thr Ala Glu Asp Leu Pro Val Phe Ala Val 
625                 630                 635                 640 


Ser Tyr Leu Val Ile Phe Leu Tyr Ile Ser Leu Ala Leu Gly Ser Tyr 
                645                 650                 655     


Ser Ser Trp Arg Arg Val Pro Val Asp Ser Lys Ala Thr Leu Gly Leu 
            660                 665                 670         


Gly Gly Val Ala Val Val Leu Gly Ala Val Val Ala Ser Met Gly Phe 
        675                 680                 685             


Phe Ser Tyr Leu Gly Val Pro Ser Ser Leu Val Ile Leu Gln Val Val 
    690                 695                 700                 


Pro Phe Leu Val Leu Ala Val Gly Ala Asp Asn Ile Phe Ile Phe Val 
705                 710                 715                 720 


Leu Glu Tyr Gln Arg Leu Pro Arg Arg Pro Gly Glu Glu Arg Glu Ala 
                725                 730                 735     


His Ile Gly Arg Ala Leu Gly Arg Val Ala Pro Ser Met Leu Leu Cys 
            740                 745                 750         


Ser Leu Ser Glu Ala Ile Cys Phe Phe Leu Gly Ala Leu Thr Pro Met 
        755                 760                 765             


Pro Ala Val Arg Thr Phe Ala Leu Thr Ser Gly Phe Ala Val Leu Leu 
    770                 775                 780                 


Asp Phe Leu Leu Gln Met Ser Ala Phe Val Ala Leu Leu Ser Leu Asp 
785                 790                 795                 800 


Ser Arg Arg Gln Glu Ala Ser Arg Met Asp Ile Cys Cys Cys Lys Thr 
                805                 810                 815     


Ala Gln Lys Leu Pro Pro Pro Ser Gln Asp Glu Gly Leu Leu Leu Arg 
            820                 825                 830         


Phe Phe Arg Lys Phe Tyr Val Pro Phe Leu Leu His Trp Leu Thr Arg 
        835                 840                 845             


Val Val Val Val Leu Leu Phe Leu Ala Leu Phe Ala Ala Ser Leu Tyr 
    850                 855                 860                 


Phe Met Cys Tyr Ile Asn Val Gly Leu Asp Gln Gln Leu Ala Leu Pro 
865                 870                 875                 880 


Lys Asp Ser Tyr Leu Ile Asp Tyr Phe Leu Phe Met Asn Arg Tyr Phe 
                885                 890                 895     


Glu Val Gly Ala Pro Val Tyr Phe Val Thr Thr Gly Gly Tyr Asn Phe 
            900                 905                 910         


Ser Ser Glu Glu Gly Met Asn Ala Ile Cys Ser Ser Ala Gly Cys Asn 
        915                 920                 925             


Asn Phe Ser Leu Thr Gln Lys Ile Gln Tyr Ala Thr Asp Phe Pro Asp 
    930                 935                 940                 


Met Ser Tyr Leu Ala Ile Pro Ala Ser Ser Trp Val Asp Asp Phe Ile 
945                 950                 955                 960 


Asp Trp Leu Thr Ser Ser Ser Cys Cys Arg Leu Tyr Ile Ser Gly Pro 
                965                 970                 975     


Asn Lys Asp Glu Phe Cys Pro Ser Thr Val Asn Ser Leu Ala Cys Leu 
            980                 985                 990         


Lys Thr Cys Val Ser Pro Thr Ala  Gly Ser Ala Arg Pro  Ser Val Glu 
        995                 1000                 1005             


Gln Phe  His Lys Tyr Leu Pro  Trp Phe Leu Ser Asp  Glu Pro Asn 
    1010                 1015                 1020             


Ile Lys  Cys Pro Lys Gly Gly  Leu Ala Ala Tyr Ser  Thr Ser Val 
    1025                 1030                 1035             


Asn Met  Ser Ser Asp Gly Gln  Ile Leu Ala Ser Arg  Phe Met Ala 
    1040                 1045                 1050             


Tyr Asn  Lys Pro Leu Lys Asn  Ser Arg Asp Phe Thr  Glu Ala Leu 
    1055                 1060                 1065             


Arg Ala  Thr Arg Ala Leu Ala  Ala Asn Ile Thr Ala  Asp Leu Arg 
    1070                 1075                 1080             


Lys Val  Pro Gly Thr Asp Pro  Asp Phe Glu Val Phe  Pro Tyr Ser 
    1085                 1090                 1095             


Val Thr  Asn Val Phe Tyr Glu  Gln Tyr Leu Thr Ile  Val Pro Glu 
    1100                 1105                 1110             


Gly Leu  Phe Met Leu Thr Ile  Cys Leu Val Pro Thr  Phe Val Val 
    1115                 1120                 1125             


Cys Cys  Phe Leu Leu Gly Met  Asp Val Arg Ser Gly  Leu Leu Asn 
    1130                 1135                 1140             


Leu Phe  Ser Ile Ile Met Ile  Leu Val Asp Thr Val  Gly Phe Met 
    1145                 1150                 1155             


Met Leu  Trp Asp Ile Ser Tyr  Asn Ala Val Ser Leu  Ile Asn Leu 
    1160                 1165                 1170             


Val Thr  Ala Val Gly Ile Ser  Val Glu Phe Val Ser  His Ile Thr 
    1175                 1180                 1185             


Arg Ser  Phe Ala Ile Ser Thr  Lys Pro Thr Arg Leu  Glu Arg Ala 
    1190                 1195                 1200             


Lys Glu  Ala Thr Ile Ser Met  Gly Ser Ala Val Phe  Ala Gly Val 
    1205                 1210                 1215             


Ala Met  Thr Asn Leu Pro Gly  Ile Leu Val Leu Gly  Leu Ala Lys 
    1220                 1225                 1230             


Ala Gln  Leu Ile Gln Ile Phe  Phe Phe Arg Leu Asn  Leu Leu Ile 
    1235                 1240                 1245             


Thr Val  Leu Gly Leu Leu His  Gly Leu Val Phe Leu  Pro Val Ile 
    1250                 1255                 1260             


Leu Ser  Tyr Leu Gly Pro Asp  Val Asn Pro Ala Leu  Val Gln Gln 
    1265                 1270                 1275             


Gln Lys  Gln Gln Glu Glu Ala  Ala Ala Ala Lys Glu  Thr Ser Cys 
    1280                 1285                 1290             


Ser Lys  His Pro Ala Gln Met  Ser Thr Asp Tyr Gly  Val Tyr Val 
    1295                 1300                 1305             


Asn Cys  Ser Phe Glu His Pro  Ala Lys Ser Val Gly  Gly Leu Glu 
    1310                 1315                 1320             


Ser Ser  Pro Ser Glu Asn Arg  Gln Lys Phe 
    1325                 1330             


<210>  16
<211>  1343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NPC1L1

<400>  16

Met Ala Glu Ala Gly Leu Arg Cys Trp Leu Leu Tyr Val Leu Leu Leu 
1               5                   10                  15      


His Leu Ala Gln Ala Gln Pro Tyr Thr Pro Ile His Gln Ala Gly Tyr 
            20                  25                  30          


Cys Ala Leu Tyr Glu Glu Cys Gly Lys Asn Pro Glu Leu Pro Gly Ser 
        35                  40                  45              


Leu Ala Glu Leu Ser Asn Val Pro Cys Leu Ser Asn Thr Pro Ala Arg 
    50                  55                  60                  


His Val Ala Gly Gln His Leu Ile Leu Leu Arg Arg Ile Cys Pro Arg 
65                  70                  75                  80  


Leu Tyr Asn Gly Leu Asn Thr Thr Tyr Ala Cys Cys Ser Ser Lys Gln 
                85                  90                  95      


Leu Glu Ser Leu Glu Leu Ser Leu Ala Ile Thr Lys Ala Leu Leu Thr 
            100                 105                 110         


Arg Cys Pro Ala Cys Ser Asp Asn Phe Val Ser Leu His Cys Gln Asn 
        115                 120                 125             


Thr Cys Ser Pro Asn Gln Ser Leu Phe Ile Asn Val Thr Arg Val Phe 
    130                 135                 140                 


Gln Leu Ala Thr Gly Gln Pro Ala Ala Val Leu Ala Tyr Gln Ala Phe 
145                 150                 155                 160 


Tyr Gln Arg Ser Phe Ala Glu His Ala Tyr Asp Ser Cys Ser Leu Val 
                165                 170                 175     


Arg Ile Pro Ala Ala Ala Ser Leu Ala Val Gly Thr Met Cys Gly Val 
            180                 185                 190         


Tyr Gly Ser Ala Leu Cys Asn Ala Gln Arg Trp Leu Asn Phe Gln Gly 
        195                 200                 205             


Asp Thr Ser Asn Gly Leu Ala Pro Leu Glu Ile Thr Phe His Leu Leu 
    210                 215                 220                 


Asp Pro Ser Gln Ala Pro Gly Asn Gly Ile Gln Leu Leu Asn Ser Glu 
225                 230                 235                 240 


Ile Val Ser Cys Asn Glu Ser Gln Gly Ile Gly Ser Pro Ala Cys Ser 
                245                 250                 255     


Cys Gln Asp Cys Ala Thr Ser Cys Pro Asp Ile Ala His Pro Gln Ala 
            260                 265                 270         


Leu Asp Ala Thr Phe Arg Phe Gly Gln Met Ala Gly Ala Leu Ala Leu 
        275                 280                 285             


Val Ile Ile Leu Cys Ser Ala Leu Val Val Leu Val Ala Phe Leu Val 
    290                 295                 300                 


Gln Pro Arg Leu Ala His Cys Arg Asp Lys Asp Lys Asp Lys Asp Lys 
305                 310                 315                 320 


Ala Val Asp Pro Gly Val His Thr Ser Phe Phe Asp Arg Leu Ser Phe 
                325                 330                 335     


Ala Thr His Lys Leu Leu Ser Arg Phe Phe Gln Ala Trp Gly Thr Trp 
            340                 345                 350         


Val Ala Ser Trp Pro Leu Thr Ile Leu Gly Val Ser Ile Ala Val Val 
        355                 360                 365             


Leu Gly Leu Ala Gly Gly Leu Ala Phe Val Glu Leu Thr Thr Asp Pro 
    370                 375                 380                 


Val Glu Leu Trp Ser Ala Pro Asn Ser Gln Ala Arg Trp Glu Lys Glu 
385                 390                 395                 400 


Phe His Asp Gln His Phe Gly Pro Phe Phe Arg Thr Asn Gln Val Ile 
                405                 410                 415     


Leu Thr Ala Pro Gly Arg Ala Ser Tyr Leu Tyr Asp Ser Leu Leu Leu 
            420                 425                 430         


Gly Pro Lys Asn Phe Ser Gly Val Leu Ser Pro Asp Leu Leu Leu Glu 
        435                 440                 445             


Leu Leu Glu Leu Gln Glu Arg Leu Arg Gln Leu Gln Val Trp Ser Pro 
    450                 455                 460                 


Lys Glu Gln Arg Asn Val Ser Leu Gly Asp Ile Cys Tyr Ala Pro Leu 
465                 470                 475                 480 


Asn Pro His Asn Ala Thr Leu Ser Asp Cys Cys Val Asn Ser Leu Leu 
                485                 490                 495     


Gln Tyr Phe Gln Asn Asn Arg Ser His Leu Leu Leu Thr Ala Asn Gln 
            500                 505                 510         


Thr Leu Met Gly Gln Thr Ser Gln Val Asp Trp Arg Asp His Phe Leu 
        515                 520                 525             


Tyr Cys Val Asn Ala Pro Leu Thr Phe Lys Asp Gly Thr Ala Leu Gly 
    530                 535                 540                 


Leu Ser Cys Met Ala Asp Tyr Gly Ala Pro Val Phe Pro Phe Leu Ala 
545                 550                 555                 560 


Val Gly Gly Tyr Lys Gly Lys Asp Tyr Ser Glu Ala Glu Ala Leu Ile 
                565                 570                 575     


Met Thr Phe Ser Leu Asn Asn Tyr Leu Pro Gly Asp Pro Arg Leu Ala 
            580                 585                 590         


Gln Val Lys Leu Trp Glu Leu Gly Phe Leu Glu Glu Val Arg Ala Phe 
        595                 600                 605             


Gln Arg Arg Met Ala Gly Thr Phe Gln Val Thr Phe Met Val Glu Arg 
    610                 615                 620                 


Ser Leu Glu Asp Glu Ile Asn Arg Thr Thr Trp Glu Asp Leu Pro Ile 
625                 630                 635                 640 


Phe Ala Thr Ser Tyr Ile Val Ile Phe Leu Tyr Ile Ala Leu Ala Leu 
                645                 650                 655     


Gly Ser Tyr Ser Ser Arg Ser Arg Arg Leu Val Asp Ser Lys Gly Thr 
            660                 665                 670         


Leu Gly Leu Gly Gly Val Ala Val Val Leu Gly Ala Val Met Ala Ala 
        675                 680                 685             


Ile Gly Phe Phe Ser Tyr Leu Gly Val Pro Ser Ser Leu Ile Ile Leu 
    690                 695                 700                 


Gln Val Val Pro Phe Leu Val Leu Ala Val Gly Ala Asp Asn Ile Phe 
705                 710                 715                 720 


Ile Phe Val Leu Glu Tyr Gln Arg Leu Pro Arg Lys Pro Gly Glu Arg 
                725                 730                 735     


Arg Glu Gln His Ile Gly Arg Thr Leu Gly His Val Gly Pro Ser Met 
            740                 745                 750         


Leu Leu Cys Ser Ile Ser Glu Ala Ile Cys Phe Phe Leu Gly Met Leu 
        755                 760                 765             


Thr Pro Met Pro Ala Val Arg Thr Phe Ala Leu Thr Cys Gly Phe Ala 
    770                 775                 780                 


Val Val Leu Asp Phe Leu Leu Gln Met Leu Ala Phe Val Ala Leu Leu 
785                 790                 795                 800 


Ser Leu Asp Ser Lys Arg Gln Glu Leu Pro Ala Leu Asp Val Cys Cys 
                805                 810                 815     


Cys Arg Arg Ala Gln Gln Pro Pro Gln Pro Ser Gln Gly Glu Gly Leu 
            820                 825                 830         


Leu Leu Arg Phe Phe Arg His Val Tyr Val Pro Val Leu Leu His Lys 
        835                 840                 845             


Val Thr Arg Ala Val Val Val Val Leu Phe Leu Ala Leu Phe Gly Val 
    850                 855                 860                 


Gly Leu Tyr Tyr Met Phe Tyr Ile Thr Val Gly Leu Asp Gln Glu Leu 
865                 870                 875                 880 


Thr Leu Pro Lys Asp Ser Tyr Leu Leu Asp Tyr Phe Leu Ser Leu Asn 
                885                 890                 895     


Arg Tyr Phe Glu Val Gly Ala Pro Ile Tyr Phe Val Thr Thr Ala Gly 
            900                 905                 910         


Tyr Asn Phe Ser Ser Thr Ala Gly Met Asn Gly Ile Cys Ser Ser Ala 
        915                 920                 925             


Gly Cys Asp Ser Tyr Ser Leu Thr Gln Lys Ile Gln Tyr Ala Thr Glu 
    930                 935                 940                 


Phe Pro Glu Leu Ser Tyr Leu Ala Met Pro Ala Ser Ser Trp Val Asp 
945                 950                 955                 960 


Asp Phe Ile Asp Trp Leu Thr Pro Ser Ser Cys Cys Arg Leu Tyr Ala 
                965                 970                 975     


Phe Gly Ser Asn Lys Asp Glu Phe Cys Pro Ser Thr Ser Ser Ser Leu 
            980                 985                 990         


Ser Cys Ser Lys Ser Cys Ile Ala  Phe Thr Leu Gly Pro  Val Arg Pro 
        995                 1000                 1005             


Ser Val  Glu Gln Phe His Lys  Tyr Leu Pro Leu Phe  Leu Asn Asp 
    1010                 1015                 1020             


Pro Pro  Asn Ile Asn Cys Pro  Lys Gly Gly Leu Ala  Ala Tyr Gly 
    1025                 1030                 1035             


Thr Ser  Val Asn Met Ser Leu  Asp Gly Gln Ile Leu  Ala Ser Arg 
    1040                 1045                 1050             


Phe Met  Ala Tyr His Thr Pro  Leu Arg Asn Ser Gln  Asp Tyr Thr 
    1055                 1060                 1065             


Glu Ala  Leu Arg Val Ser Arg  Ala Leu Ala Ala Asn  Ile Thr Ala 
    1070                 1075                 1080             


Ser Leu  Arg Lys Val Pro Gly  Thr Asp Pro Ala Phe  Glu Val Phe 
    1085                 1090                 1095             


Pro Tyr  Thr Ile Ser Asn Val  Phe Tyr Glu Gln Tyr  Leu Met Val 
    1100                 1105                 1110             


Val Pro  Glu Gly Leu Phe Met  Leu Gly Leu Cys Leu  Val Pro Thr 
    1115                 1120                 1125             


Phe Ala  Val Cys Cys Leu Leu  Leu Gly Met Asp Leu  Arg Ser Gly 
    1130                 1135                 1140             


Leu Ile  Asn Leu Phe Ser Ile  Val Met Ile Leu Val  Asp Thr Val 
    1145                 1150                 1155             


Gly Phe  Met Ala Leu Trp Gly  Ile Ser Tyr Asn Thr  Val Ser Leu 
    1160                 1165                 1170             


Ile Asn  Leu Val Ser Ala Val  Gly Ile Ser Val Glu  Phe Val Ser 
    1175                 1180                 1185             


His Ile  Thr Arg Ser Phe Ala  Ile Ser Ile Lys Pro  Thr Arg Leu 
    1190                 1195                 1200             


Glu Arg  Ala Lys Glu Ala Thr  Ile Thr Met Gly Ser  Ala Val Phe 
    1205                 1210                 1215             


Ala Gly  Val Ala Met Thr Asn  Leu Pro Gly Ile Leu  Val Leu Gly 
    1220                 1225                 1230             


Leu Ala  Lys Ala Gln Leu Ile  Gln Ile Phe Phe Phe  His Leu Asn 
    1235                 1240                 1245             


Leu Leu  Ile Thr Val Leu Gly  Leu Leu His Gly Leu  Val Phe Leu 
    1250                 1255                 1260             


Pro Val  Val Leu Ser Tyr Leu  Gly Pro Asp Val Asn  Arg Ala Leu 
    1265                 1270                 1275             


Val Leu  Glu Gln Arg Gln Ala  Glu Val Ala Ala Thr  Ala Lys Glu 
    1280                 1285                 1290             


Ala Thr  Ala Arg Glu Asn Pro  Cys Leu Thr Tyr Pro  Ser Val Thr 
    1295                 1300                 1305             


His Met  Thr Asn Asn Val Tyr  Ile Asn Glu Asp Phe  Glu His Asp 
    1310                 1315                 1320             


Val Lys  Ser Ala Asn Asp Val  Asp Ser Ser Cys Pro  Ile Gly Leu 
    1325                 1330                 1335             


Arg Val  Leu Cys Pro 
    1340             


<210>  17
<211>  1333
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NPC1L1

<400>  17

Met Ala Ala Ala Trp Gln Gly Trp Leu Leu Trp Ala Leu Leu Leu Asn 
1               5                   10                  15      


Ser Ala Gln Gly Glu Leu Tyr Thr Pro Thr His Lys Ala Gly Phe Cys 
            20                  25                  30          


Thr Phe Tyr Glu Glu Cys Gly Lys Asn Pro Glu Leu Ser Gly Gly Leu 
        35                  40                  45              


Thr Ser Leu Ser Asn Ile Ser Cys Leu Ser Asn Thr Pro Ala Arg His 
    50                  55                  60                  


Val Thr Gly Asp His Leu Ala Leu Leu Gln Arg Val Cys Pro Arg Leu 
65                  70                  75                  80  


Tyr Asn Gly Pro Asn Asp Thr Tyr Ala Cys Cys Ser Thr Lys Gln Leu 
                85                  90                  95      


Val Ser Leu Asp Ser Ser Leu Ser Ile Thr Lys Ala Leu Leu Thr Arg 
            100                 105                 110         


Cys Pro Ala Cys Ser Glu Asn Phe Val Ser Ile His Cys His Asn Thr 
        115                 120                 125             


Cys Ser Pro Asp Gln Ser Leu Phe Ile Asn Val Thr Arg Val Val Gln 
    130                 135                 140                 


Arg Asp Pro Gly Gln Leu Pro Ala Val Val Ala Tyr Glu Ala Phe Tyr 
145                 150                 155                 160 


Gln Arg Ser Phe Ala Glu Lys Ala Tyr Glu Ser Cys Ser Arg Val Arg 
                165                 170                 175     


Ile Pro Ala Ala Ala Ser Leu Ala Val Gly Ser Met Cys Gly Val Tyr 
            180                 185                 190         


Gly Ser Ala Leu Cys Asn Ala Gln Arg Trp Leu Asn Phe Gln Gly Asp 
        195                 200                 205             


Thr Gly Asn Gly Leu Ala Pro Leu Asp Ile Thr Phe His Leu Leu Glu 
    210                 215                 220                 


Pro Gly Gln Ala Leu Ala Asp Gly Met Lys Pro Leu Asp Gly Lys Ile 
225                 230                 235                 240 


Thr Pro Cys Asn Glu Ser Gln Gly Glu Asp Ser Ala Ala Cys Ser Cys 
                245                 250                 255     


Gln Asp Cys Ala Ala Ser Cys Pro Val Ile Pro Pro Pro Pro Ala Leu 
            260                 265                 270         


Arg Pro Ser Phe Tyr Met Gly Arg Met Pro Gly Trp Leu Ala Leu Ile 
        275                 280                 285             


Ile Ile Phe Thr Ala Val Phe Val Leu Leu Ser Val Val Leu Val Tyr 
    290                 295                 300                 


Leu Arg Val Ala Ser Asn Arg Asn Lys Asn Lys Thr Ala Gly Ser Gln 
305                 310                 315                 320 


Glu Ala Pro Asn Leu Pro Arg Lys Arg Arg Phe Ser Pro His Thr Val 
                325                 330                 335     


Leu Gly Arg Phe Phe Glu Ser Trp Gly Thr Arg Val Ala Ser Trp Pro 
            340                 345                 350         


Leu Thr Val Leu Ala Leu Ser Phe Ile Val Val Ile Ala Leu Ser Val 
        355                 360                 365             


Gly Leu Thr Phe Ile Glu Leu Thr Thr Asp Pro Val Glu Leu Trp Ser 
    370                 375                 380                 


Ala Pro Lys Ser Gln Ala Arg Lys Glu Lys Ala Phe His Asp Glu His 
385                 390                 395                 400 


Phe Gly Pro Phe Phe Arg Thr Asn Gln Ile Phe Val Thr Ala Lys Asn 
                405                 410                 415     


Arg Ser Ser Tyr Lys Tyr Asp Ser Leu Leu Leu Gly Pro Lys Asn Phe 
            420                 425                 430         


Ser Gly Ile Leu Ser Leu Asp Leu Leu Gln Glu Leu Leu Glu Leu Gln 
        435                 440                 445             


Glu Arg Leu Arg His Leu Gln Val Trp Ser His Glu Ala Gln Arg Asn 
    450                 455                 460                 


Ile Ser Leu Gln Asp Ile Cys Tyr Ala Pro Leu Asn Pro His Asn Thr 
465                 470                 475                 480 


Ser Leu Thr Asp Cys Cys Val Asn Ser Leu Leu Gln Tyr Phe Gln Asn 
                485                 490                 495     


Asn His Thr Leu Leu Leu Leu Thr Ala Asn Gln Thr Leu Asn Gly Gln 
            500                 505                 510         


Thr Ser Leu Val Asp Trp Lys Asp His Phe Leu Tyr Cys Ala Asn Ala 
        515                 520                 525             


Pro Leu Thr Tyr Lys Asp Gly Thr Ala Leu Ala Leu Ser Cys Ile Ala 
    530                 535                 540                 


Asp Tyr Gly Ala Pro Val Phe Pro Phe Leu Ala Val Gly Gly Tyr Gln 
545                 550                 555                 560 


Gly Thr Asp Tyr Ser Glu Ala Glu Ala Leu Ile Ile Thr Phe Ser Ile 
                565                 570                 575     


Asn Asn Tyr Pro Ala Asp Asp Pro Arg Met Ala His Ala Lys Leu Trp 
            580                 585                 590         


Glu Glu Ala Phe Leu Lys Glu Met Gln Ser Phe Gln Arg Ser Thr Ala 
        595                 600                 605             


Asp Lys Phe Gln Ile Ala Phe Ser Ala Glu Arg Ser Leu Glu Asp Glu 
    610                 615                 620                 


Ile Asn Arg Thr Thr Ile Gln Asp Leu Pro Val Phe Ala Ile Ser Tyr 
625                 630                 635                 640 


Leu Ile Val Phe Leu Tyr Ile Ser Leu Ala Leu Gly Ser Tyr Ser Arg 
                645                 650                 655     


Trp Ser Arg Val Ala Val Asp Ser Lys Ala Thr Leu Gly Leu Gly Gly 
            660                 665                 670         


Val Ala Val Val Leu Gly Ala Val Val Ala Ala Met Gly Phe Tyr Ser 
        675                 680                 685             


Tyr Leu Gly Val Pro Ser Ser Leu Val Ile Ile Gln Val Val Pro Phe 
    690                 695                 700                 


Leu Val Leu Ala Val Gly Ala Asp Asn Ile Phe Ile Phe Val Leu Glu 
705                 710                 715                 720 


Tyr Gln Arg Leu Pro Arg Met Pro Gly Glu Gln Arg Glu Ala His Ile 
                725                 730                 735     


Gly Arg Thr Leu Gly Ser Val Ala Pro Ser Met Leu Leu Cys Ser Leu 
            740                 745                 750         


Ser Glu Ala Ile Cys Phe Phe Leu Gly Ala Leu Thr Ser Met Pro Ala 
        755                 760                 765             


Val Arg Thr Phe Ala Leu Thr Ser Gly Leu Ala Ile Ile Phe Asp Phe 
    770                 775                 780                 


Leu Leu Gln Met Thr Ala Phe Val Ala Leu Leu Ser Leu Asp Ser Lys 
785                 790                 795                 800 


Arg Gln Glu Ala Ser Arg Pro Asp Val Val Cys Cys Phe Ser Ser Arg 
                805                 810                 815     


Asn Leu Pro Pro Pro Lys Gln Lys Glu Gly Leu Leu Leu Cys Phe Phe 
            820                 825                 830         


Arg Lys Ile Tyr Thr Pro Phe Leu Leu His Arg Phe Ile Arg Pro Val 
        835                 840                 845             


Val Leu Leu Leu Phe Leu Val Leu Phe Gly Ala Asn Leu Tyr Leu Met 
    850                 855                 860                 


Cys Asn Ile Ser Val Gly Leu Asp Gln Asp Leu Ala Leu Pro Lys Asp 
865                 870                 875                 880 


Ser Tyr Leu Ile Asp Tyr Phe Leu Phe Leu Asn Arg Tyr Leu Glu Val 
                885                 890                 895     


Gly Pro Pro Val Tyr Phe Asp Thr Thr Ser Gly Tyr Asn Phe Ser Thr 
            900                 905                 910         


Glu Ala Gly Met Asn Ala Ile Cys Ser Ser Ala Gly Cys Glu Ser Phe 
        915                 920                 925             


Ser Leu Thr Gln Lys Ile Gln Tyr Ala Ser Glu Phe Pro Asn Gln Ser 
    930                 935                 940                 


Tyr Val Ala Ile Ala Ala Ser Ser Trp Val Asp Asp Phe Ile Asp Trp 
945                 950                 955                 960 


Leu Thr Pro Ser Ser Ser Cys Cys Arg Ile Tyr Thr Arg Gly Pro His 
                965                 970                 975     


Lys Asp Glu Phe Cys Pro Ser Thr Asp Thr Ser Phe Asn Cys Leu Lys 
            980                 985                 990         


Asn Cys Met Asn Arg Thr Leu Gly  Pro Val Arg Pro Thr  Thr Glu Gln 
        995                 1000                 1005             


Phe His  Lys Tyr Leu Pro Trp  Phe Leu Asn Asp Thr  Pro Asn Ile 
    1010                 1015                 1020             


Arg Cys  Pro Lys Gly Gly Leu  Ala Ala Tyr Arg Thr  Ser Val Asn 
    1025                 1030                 1035             


Leu Ser  Ser Asp Gly Gln Ile  Ile Ala Ser Gln Phe  Met Ala Tyr 
    1040                 1045                 1050             


His Lys  Pro Leu Arg Asn Ser  Gln Asp Phe Thr Glu  Ala Leu Arg 
    1055                 1060                 1065             


Ala Ser  Arg Leu Leu Ala Ala  Asn Ile Thr Ala Glu  Leu Arg Lys 
    1070                 1075                 1080             


Val Pro  Gly Thr Asp Pro Asn  Phe Glu Val Phe Pro  Tyr Thr Ile 
    1085                 1090                 1095             


Ser Asn  Val Phe Tyr Gln Gln  Tyr Leu Thr Val Leu  Pro Glu Gly 
    1100                 1105                 1110             


Ile Phe  Thr Leu Ala Leu Cys  Phe Val Pro Thr Phe  Val Val Cys 
    1115                 1120                 1125             


Tyr Leu  Leu Leu Gly Leu Asp  Ile Arg Ser Gly Ile  Leu Asn Leu 
    1130                 1135                 1140             


Leu Ser  Ile Ile Met Ile Leu  Val Asp Thr Ile Gly  Leu Met Ala 
    1145                 1150                 1155             


Val Trp  Gly Ile Ser Tyr Asn  Ala Val Ser Leu Ile  Asn Leu Val 
    1160                 1165                 1170             


Thr Ala  Val Gly Met Ser Val  Glu Phe Val Ser His  Ile Thr Arg 
    1175                 1180                 1185             


Ser Phe  Ala Val Ser Thr Lys  Pro Thr Arg Leu Glu  Arg Ala Lys 
    1190                 1195                 1200             


Asp Ala  Thr Ile Phe Met Gly  Ser Ala Val Phe Ala  Gly Val Ala 
    1205                 1210                 1215             


Met Thr  Asn Phe Pro Gly Ile  Leu Ile Leu Gly Phe  Ala Gln Ala 
    1220                 1225                 1230             


Gln Leu  Ile Gln Ile Phe Phe  Phe Arg Leu Asn Leu  Leu Ile Thr 
    1235                 1240                 1245             


Leu Leu  Gly Leu Leu His Gly  Leu Val Phe Leu Pro  Val Val Leu 
    1250                 1255                 1260             


Ser Tyr  Leu Gly Pro Asp Val  Asn Gln Ala Leu Val  Leu Glu Glu 
    1265                 1270                 1275             


Lys Leu  Ala Thr Glu Ala Ala  Met Val Ser Glu Pro  Ser Cys Pro 
    1280                 1285                 1290             


Gln Tyr  Pro Phe Pro Ala Asp  Ala Asn Thr Ser Asp  Tyr Val Asn 
    1295                 1300                 1305             


Tyr Gly  Phe Asn Pro Glu Phe  Ile Pro Glu Ile Asn  Ala Ala Ser 
    1310                 1315                 1320             


Ser Ser  Leu Pro Lys Ser Asp  Gln Lys Phe 
    1325                 1330             


<210>  18
<211>  1315
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NPC1L1

<400>  18

Met Val Glu Pro Gly Leu Arg Gly Val Leu Leu Leu Val Val Leu Leu 
1               5                   10                  15      


His Leu Val His Gly Glu Ser Tyr Thr Pro Ile His Glu Ala Gly Arg 
            20                  25                  30          


Cys Ala Phe Tyr Asp Asp Cys Gly Lys Asn Pro Glu Leu Ser Gly Ser 
        35                  40                  45              


Leu Ile Pro Leu Ala Asn Val Ser Cys Leu Ser Asn Thr Pro Ala Phe 
    50                  55                  60                  


Lys Leu Ser Gly Asp His Leu Glu Leu Leu Asn Lys Ile Cys Pro Glu 
65                  70                  75                  80  


Leu Tyr Gln Gly Pro Asp Ser Thr Tyr Ala Cys Cys Ser Ile Gln Gln 
                85                  90                  95      


Leu Leu Asp Leu Gln Gly Ser Leu Ser Ile Ser Lys Thr Leu Leu Thr 
            100                 105                 110         


Arg Cys Pro Ala Cys Ala Glu Asn Phe Ala Ala Phe Tyr Cys His Asn 
        115                 120                 125             


Thr Cys Ser Pro Asn Gln Ser Val Phe Ile Asn Val Thr Arg Ile Ala 
    130                 135                 140                 


Asn Ile Ser Gly Thr Asn Ser Ser Lys Val Leu Ala Tyr Glu Thr Phe 
145                 150                 155                 160 


Tyr Thr Arg Asp Phe Ala Glu Arg Ala Tyr Asn Ser Cys Asn Lys Val 
                165                 170                 175     


Gln Ile Pro Ala Thr Gly Gly Leu Ala Val Gly Ala Met Cys Gly Val 
            180                 185                 190         


Tyr Gly Ala Lys Leu Cys Asn Ala Gln Arg Trp Leu Asn Phe Gln Gly 
        195                 200                 205             


Asp Thr Gly Asn Gly Leu Ala Pro Leu Glu Val Thr Phe Asn Leu Val 
    210                 215                 220                 


Asp Pro Gly Gln Val Leu Gly Asn Gly Leu Glu Pro Leu Asp Val Glu 
225                 230                 235                 240 


Thr Trp Gly Cys Asn Glu Thr Arg Gly Asn Gly Ser Asn Ala Cys Ser 
                245                 250                 255     


Cys Gln Asp Cys Ala Ser Ser Cys Pro Val Ile Ala Gln Pro Ala Met 
            260                 265                 270         


Gln Asp Ser Thr Phe Arg Leu Gly Arg Met His Gly Gly Leu Ala Leu 
        275                 280                 285             


Ala Ile Leu Leu Ser Ala Leu Phe Val Ser Phe Phe Thr Phe Leu Val 
    290                 295                 300                 


Val Arg Ser Cys Gln Lys Thr Lys Ala Lys Lys Pro Lys Gln Gly Val 
305                 310                 315                 320 


Arg Cys Ser Asp Arg Phe Ser Tyr Phe Ala His Val Ile Leu Gly Asn 
                325                 330                 335     


Leu Phe Gln Ser Trp Gly Thr Trp Val Ala Ser Arg Pro Lys Ile Val 
            340                 345                 350         


Leu Cys Leu Ser Thr Ile Leu Val Val Ala Leu Ala Ser Gly Leu Val 
        355                 360                 365             


Phe Leu Glu Leu Thr Thr Asn Pro Val Glu Leu Trp Ser Ala Pro Asn 
    370                 375                 380                 


Ser Gln Ala Arg Lys Glu Lys Glu Phe His Asp Arg Tyr Phe Gly Pro 
385                 390                 395                 400 


Phe Phe Arg Thr Asn Gln Val Ile Leu Thr Ala Pro Asn Arg Pro Ser 
                405                 410                 415     


Tyr Thr Tyr Asn Ser Leu Leu Leu Gly Pro Lys Asn Phe Ser Gly Ile 
            420                 425                 430         


Leu Ser Glu Asp Leu Leu Leu Gln Leu Leu Glu Leu Gln Glu Glu Leu 
        435                 440                 445             


Arg His Met Glu Val Trp Ser Glu Glu Glu Arg Arg Asn Ile Ser Leu 
    450                 455                 460                 


Arg Asp Val Cys Tyr Ala Pro Leu Asn Pro Arg Asn Pro Ser Leu Glu 
465                 470                 475                 480 


Asp Cys Cys Ile Asn Ser Phe Leu Gln Tyr Phe Gln Ser Asn Arg Thr 
                485                 490                 495     


Asn Phe Leu Leu Thr Ala Asn Gln Thr Leu Met Gly His Thr Ala Gln 
            500                 505                 510         


Val Asp Trp Arg Asp His Tyr Leu Tyr Cys Val Asn Ser Pro Leu Thr 
        515                 520                 525             


Phe Lys Asp Gly Thr Thr Leu Ala Leu Ser Cys Met Ala Asp Tyr Gly 
    530                 535                 540                 


Ala Pro Val Phe Pro Phe Leu Ala Val Gly Gly Tyr Thr Gly Gln Asp 
545                 550                 555                 560 


Phe Ser Ala Ala Glu Ala Leu Leu Leu Thr Phe Ser Leu Asn Asn Tyr 
                565                 570                 575     


Pro Ala Gly Asp Pro Arg Leu Ala Gln Thr Leu Leu Trp Glu Ser Arg 
            580                 585                 590         


Phe Leu Glu Val Met Arg Asp Phe Gln Lys Arg Thr Ala Gly Thr Phe 
        595                 600                 605             


Asp Val Thr Phe Met Ala Glu Arg Ser Leu Glu Asp Glu Ile Asn Arg 
    610                 615                 620                 


Thr Thr Ala Glu Asp Leu Pro Val Phe Ala Ile Ser Tyr Ala Ile Val 
625                 630                 635                 640 


Phe Leu Tyr Ile Ser Leu Ala Leu Gly Arg Tyr Ser Ser Arg Ser Arg 
                645                 650                 655     


Leu Leu Val Asp Ser Lys Ile Thr Leu Gly Leu Gly Gly Val Leu Ile 
            660                 665                 670         


Val Leu Gly Ser Val Leu Ser Ser Met Gly Phe Phe Ser Tyr Leu Gly 
        675                 680                 685             


Val Pro Ser Ser Met Val Ile Ile Gln Val Val Pro Phe Leu Val Leu 
    690                 695                 700                 


Ala Val Gly Ala Asp Asn Ile Phe Ile Phe Val Leu Glu Tyr Gln Arg 
705                 710                 715                 720 


Leu Pro Arg His Ser Glu Glu Ser Arg Glu Val His Ile Gly Arg Val 
                725                 730                 735     


Leu Gly Thr Val Ala Pro Ser Met Leu Leu Cys Ser Leu Ser Glu Ala 
            740                 745                 750         


Ile Cys Phe Phe Leu Gly Ala Leu Thr Ser Met Pro Ala Val Arg Thr 
        755                 760                 765             


Phe Ala Leu Thr Ala Gly Leu Ala Ile Ile Phe Asp Phe Leu Leu Gln 
    770                 775                 780                 


Met Ser Ala Phe Val Ala Leu Leu Ser Leu Asp Ser Lys Arg Gln Glu 
785                 790                 795                 800 


Ala Ser Arg Leu Asp Ile Cys Cys Cys Val Asn Pro Arg Lys Leu Pro 
                805                 810                 815     


Pro Pro Gln Glu Asp Glu Ser Leu Leu Leu Cys Phe Phe Arg Lys Phe 
            820                 825                 830         


Tyr Ala Pro Phe Leu Leu His Gly Phe Thr Arg Ile Ile Val Leu Gly 
        835                 840                 845             


Leu Phe Met Ala Leu Phe Gly Gly Ser Leu Tyr Leu Met Cys His Ile 
    850                 855                 860                 


Asn Val Gly Leu Asp Gln Glu Leu Ala Leu Pro Glu Asp Ser Tyr Leu 
865                 870                 875                 880 


Leu Asp Tyr Phe His Phe Leu Asn Arg Tyr Phe Glu Val Gly Val Pro 
                885                 890                 895     


Thr Tyr Phe Val Thr Thr Ser Gly Phe Asn Phe Ser Ser Leu Asp Gly 
            900                 905                 910         


Met Asn Ser Ile Cys Ser Ser Ser Gly Cys Asp Thr Tyr Ser Leu Thr 
        915                 920                 925             


Gln Lys Ile Gln Tyr Ala Thr Glu Phe Pro Lys Glu Ser Tyr Leu Ala 
    930                 935                 940                 


Ile Pro Ala Ser Ser Trp Val Asp Asp Phe Ile Asp Trp Leu Thr Pro 
945                 950                 955                 960 


Thr Ser Asp Cys Cys Arg Leu Tyr Arg Asn Asn Thr Phe Cys Ser Ser 
                965                 970                 975     


Thr Glu Gly Thr Val Leu Thr Cys Phe Arg Lys Cys Ile Pro Tyr Thr 
            980                 985                 990         


Thr Ser Thr Val Arg Pro Thr Val  Glu Gln Phe His Lys  Tyr Leu Pro 
        995                 1000                 1005             


Trp Phe  Leu Glu Asp Lys Ala  Asn Ile Lys Cys Pro  Lys Gly Gly 
    1010                 1015                 1020             


Leu Gly  Ala Tyr Asp Gly Ala  Val Asn Ile Ser Ala  Asp Gly Glu 
    1025                 1030                 1035             


Ile Leu  Ala Thr Arg Phe Met  Ala Tyr His Thr Pro  Leu Lys Asn 
    1040                 1045                 1050             


Ser Gln  Glu Tyr Thr Ala Ala  Leu Arg Ala Ala Arg  Glu Leu Ala 
    1055                 1060                 1065             


Ala Asn  Ile Thr Ala Asp Leu  Arg Arg Val Pro Gly  Thr Asp Pro 
    1070                 1075                 1080             


Lys Phe  Glu Val Phe Pro Tyr  Thr Val Thr Tyr Val  Phe Tyr Glu 
    1085                 1090                 1095             


Gln Tyr  Leu Thr Ile Val Pro  Glu Gly Leu Phe Ile  Leu Gly Leu 
    1100                 1105                 1110             


Cys Leu  Ile Pro Thr Phe Ala  Val Ser Cys Leu Leu  Leu Gly Met 
    1115                 1120                 1125             


Asp Leu  Arg Ser Gly Leu Leu  Asn Leu Phe Ser Ile  Ile Met Ile 
    1130                 1135                 1140             


Leu Val  Asp Thr Val Gly Phe  Met Thr Leu Trp Gly  Ile Ser Tyr 
    1145                 1150                 1155             


Asn Ala  Val Ser Leu Ile Asn  Leu Val Thr Ala Val  Gly Ile Ser 
    1160                 1165                 1170             


Val Glu  Phe Val Ser His Ile  Thr Arg Ser Phe Ala  Ile Ser Thr 
    1175                 1180                 1185             


Lys Pro  Ser Arg Leu Glu Arg  Ala Lys Glu Thr Thr  Ile Ile Met 
    1190                 1195                 1200             


Gly Ser  Ala Val Phe Ala Gly  Val Ala Met Thr Asn  Leu Pro Gly 
    1205                 1210                 1215             


Ile Ile  Ile Leu Ala Ala Ala  Lys Ala Gln Leu Ile  Gln Ile Phe 
    1220                 1225                 1230             


Phe Phe  Arg Leu Asn Leu Leu  Ile Thr Ile Phe Gly  Met Leu His 
    1235                 1240                 1245             


Gly Leu  Val Phe Leu Pro Val  Ile Leu Ser Tyr Phe  Gly Pro Asp 
    1250                 1255                 1260             


Val Lys  Gln Val Leu Val Leu  Glu Gln Gln Pro Ile  Glu Lys Asn 
    1265                 1270                 1275             


Thr Met  Ser Gly Lys Ala Glu  Val Ala Lys Ala Ala  Ser Cys Ser 
    1280                 1285                 1290             


Thr His  Pro Ser Pro Leu Tyr  Pro Asp Leu Asn Ser  Tyr Thr Asn 
    1295                 1300                 1305             


Leu Asn  Phe Glu Ala Asp Val  
    1310                 1315 


<210>  19
<211>  1227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NPC1L1


<220>
<221>  misc_feature
<222>  (577)..(577)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (590)..(590)
<223>  Xaa can be any naturally occurring amino acid

<400>  19

Met Ala Glu Ala Ser Leu Arg Gly Trp Leu Leu Trp Ala Leu Leu Val 
1               5                   10                  15      


Leu Ser Thr Gln Gly Gln Pro Tyr Thr Pro Ile His Arg Ala Gly Thr 
            20                  25                  30          


Cys Ala Phe Tyr Asp Glu Cys Gly Lys Asn Pro Glu Leu Ser Gly Gly 
        35                  40                  45              


Leu Ala Pro Leu Ser Asn Val Ser Cys Leu Ser Asn Thr Pro Ala Arg 
    50                  55                  60                  


Leu Val Thr Gly Gln His Leu Val Leu Leu Gln Arg Ile Cys Pro Ser 
65                  70                  75                  80  


Leu Tyr Ser Gly Pro Ser Thr Thr Tyr Ala Cys Cys Ser Pro Glu Gln 
                85                  90                  95      


Leu Val Ser Leu Glu Leu Ser Leu Thr Leu Ser Lys Ala Leu Leu Thr 
            100                 105                 110         


Arg Cys Pro Ala Cys Ala Asp Asn Phe Gly Ser Leu His Cys His Asn 
        115                 120                 125             


Thr Cys Ser Pro Asn Gln Ser Leu Phe Val Asn Val Thr Arg Val Ala 
    130                 135                 140                 


Glu Arg Gly Pro Gly Glu Leu Pro Ala Val Val Ala Tyr Glu Ala Tyr 
145                 150                 155                 160 


Tyr Arg Arg Ser Phe Ala Glu Arg Thr Tyr Asp Ser Cys Ser Arg Val 
                165                 170                 175     


Arg Ile Pro Ala Ala Ala Thr Leu Ala Val Gly Ala Met Cys Gly Val 
            180                 185                 190         


Tyr Gly Ser Ala Leu Cys Asn Ala Gln Arg Trp Leu Asn Phe Gln Gly 
        195                 200                 205             


Asp Thr Ser Asn Gly Leu Ala Pro Leu Asp Leu Thr Phe His Leu Trp 
    210                 215                 220                 


Asp Ala Asp Gln Ser Pro Gly Asn Gly Ile Gln Pro Leu Asp Glu Glu 
225                 230                 235                 240 


Val Val Ser Cys Asn Arg Ser Gln Glu Gly Gly Gly Val Ala Cys Ser 
                245                 250                 255     


Cys Gln Asp Cys Thr Ala Ala Cys Pro Ala Ile Ala Gln Pro Leu Pro 
            260                 265                 270         


Leu Asp Ala Thr Phe Arg Met Gly His Met Ala Gly Gly Leu Ala Leu 
        275                 280                 285             


Val Ile Ile Leu Val Ser Val Ser Val Val Leu Ala Ala Phe Leu Val 
    290                 295                 300                 


Arg Pro Arg Val Ala Ala Cys Arg Gly Lys Ala Ser Pro Ala Ala Pro 
305                 310                 315                 320 


Gly Pro His Thr Ser Phe Leu Asp Arg Leu Ser Leu Ser Thr His Thr 
                325                 330                 335     


Phe Leu Gly Gly Leu Phe Gln Gly Trp Gly Thr Arg Val Ala Ser Trp 
            340                 345                 350         


Pro Leu Thr Val Leu Ala Val Ser Val Ala Val Val Ala Ala Leu Ala 
        355                 360                 365             


Gly Gly Leu Ala Phe Thr Glu Leu Thr Thr Asp Pro Val Glu Leu Trp 
    370                 375                 380                 


Ser Ala Pro Lys Ser Gln Ala Arg Leu Glu Lys Glu Phe His Asp Arg 
385                 390                 395                 400 


His Phe Gly Pro Phe Phe Arg Thr Asn Gln Ala Ile Leu Thr Ala Pro 
                405                 410                 415     


Ser Arg Ala Ala Tyr Arg Tyr Asp Ser Leu Leu Leu Gly Pro Lys Asn 
            420                 425                 430         


Phe Ser Gly Ile Leu Ser Leu Asp Leu Leu Leu Glu Leu Leu Glu Leu 
        435                 440                 445             


Gln Glu Lys Leu Arg His Leu Gln Val Trp Ser Pro Glu Glu Gln Arg 
    450                 455                 460                 


Asn Ile Ser Leu Gln Asp Ile Cys Tyr Ala Pro Leu Ser Pro His Asn 
465                 470                 475                 480 


Ala Ser Leu Ser Asp Cys Cys Val Asn Ser Leu Val Gln Tyr Phe Gln 
                485                 490                 495     


Asn Asn Arg Thr Asn Leu Leu Leu Thr Ala Asn Gln Thr Leu Met Gly 
            500                 505                 510         


Gln Thr Ala Gln Ile Asp Trp Arg Asp His Phe Leu Tyr Cys Val Lys 
        515                 520                 525             


Arg Trp Leu Asp Ser Asp Ser Trp Phe Cys Val Pro Ser Val Gly Thr 
    530                 535                 540                 


Cys Trp Leu Lys Trp Lys Asp Tyr Ser Glu Ala Glu Ala Leu Ile Met 
545                 550                 555                 560 


Thr Phe Ser Leu Asn Asn Tyr Pro Ala Gly Asp Arg Arg Leu Ala Arg 
                565                 570                 575     


Xaa Lys Leu Trp Glu Arg Gly Phe Leu Asp Glu Met Gln Xaa Phe Gln 
            580                 585                 590         


Leu Arg Thr Ala Gly Lys Phe Gln Val Thr Phe Met Ala Glu Arg Ser 
        595                 600                 605             


Leu Glu Asp Glu Ile Asn Arg Thr Thr Ala Glu Asp Leu Pro Ile Phe 
    610                 615                 620                 


Ala Ile Ser Tyr Ile Ile Ile Phe Leu Tyr Ile Ser Leu Ala Leu Gly 
625                 630                 635                 640 


Ser Tyr Ser Ser Arg His Arg Val Leu Val Asp Ser Lys Ala Thr Leu 
                645                 650                 655     


Gly Leu Gly Gly Val Ala Val Val Leu Gly Ala Val Val Ala Ala Met 
            660                 665                 670         


Gly Phe Phe Ser Tyr Leu Gly Val Pro Ser Ser Leu Val Ile Ile Gln 
        675                 680                 685             


Val Val Pro Phe Leu Val Leu Ala Val Gly Ala Asp Asn Val Phe Ile 
    690                 695                 700                 


Phe Val Leu Glu Tyr Gln Arg Leu Pro Arg Gln Pro Gly Glu Gln Pro 
705                 710                 715                 720 


Glu Ala His Ile Gly Arg Ala Leu Gly Arg Val Ala Pro Ser Met Leu 
                725                 730                 735     


Leu Cys Ser Leu Ser Glu Ala Val Cys Phe Phe Leu Gly Ala Leu Thr 
            740                 745                 750         


Pro Met Pro Ala Val Arg Thr Phe Ala Leu Thr Thr Gly Leu Ala Val 
        755                 760                 765             


Val Phe Asp Phe Leu Leu Gln Val Ser Ala Phe Val Ala Leu Leu Ser 
    770                 775                 780                 


Leu Asp Cys Arg Arg Gln Glu Asp Ser Arg Leu Asp Val Cys Cys Cys 
785                 790                 795                 800 


Val Arg Ala Arg Lys Leu Pro Pro Pro Asp Pro Gly Glu Gly Leu Leu 
                805                 810                 815     


Leu Arg Phe Phe Arg Lys Thr Tyr Ala Pro Phe Leu Leu His Arg Val 
            820                 825                 830         


Thr Arg Gly Val Val Leu Ala Ile Phe Val Ala Leu Phe Gly Val Ser 
        835                 840                 845             


Leu Tyr Phe Met Cys His Leu Ser Val Gly Leu Asp Gln Glu Leu Ala 
    850                 855                 860                 


Leu Pro Gln Asp Ser Tyr Leu Leu Asp Tyr Phe Leu Phe Leu Asn Arg 
865                 870                 875                 880 


Tyr Phe Glu Val Gly Ala Pro Val Tyr Phe Val Thr Thr Gly Gly Tyr 
                885                 890                 895     


Asn Phe Ser Ser Met Ala Gly Met Asn Gly Ile Cys Ser Ser Ala Gly 
            900                 905                 910         


Cys Asp Asn Phe Ser Leu Thr Gln Lys Ile Gln Tyr Ala Thr Asp Phe 
        915                 920                 925             


Pro Glu Glu Ser Tyr Leu Ala Ile Pro Ala Ser Ser Trp Val Asp Asp 
    930                 935                 940                 


Phe Ile Asp Trp Leu Thr Thr Ser Ser Cys Cys Arg Leu Tyr Ala Phe 
945                 950                 955                 960 


Gly Pro Asn Lys Asp Glu Phe Cys Pro Ser Thr Ile Ser Ser Leu Ala 
                965                 970                 975     


Cys Ile Lys Lys Cys Val Ser Val Thr Leu Gly Pro Leu Arg Pro Ser 
            980                 985                 990         


Val Glu Gln Phe His Arg Tyr Leu  Pro Trp Phe Leu Ser  Asp Pro Pro 
        995                 1000                 1005             


Asn Ile  Lys Cys Pro Lys Gly  Gly Leu Ala Ala Tyr  Asn Thr Ile 
    1010                 1015                 1020             


Leu Ala  Thr Arg Phe Met Ala  Tyr Ser Lys Pro Leu  Lys Asn Ser 
    1025                 1030                 1035             


Arg Asp  Tyr Thr Glu Ala Leu  Arg Ala Thr Arg Ala  Leu Ala Ala 
    1040                 1045                 1050             


Asn Ile  Thr Ala Ser Leu Arg  Ala Val Pro Gly Thr  Asp Pro Ala 
    1055                 1060                 1065             


Phe Glu  Val Phe Pro Tyr Thr  Asp Ile Ser Tyr Asn  Ala Val Ser 
    1070                 1075                 1080             


Leu Ile  Asn Leu Val Thr Ala  Val Gly Ile Ser Val  Glu Phe Val 
    1085                 1090                 1095             


Ser His  Ile Thr Arg Ser Phe  Ala Ile Ser Thr Lys  Pro Thr Arg 
    1100                 1105                 1110             


Leu Glu  Arg Ala Glu Glu Ala  Thr Val Thr Met Gly  Ser Ala Val 
    1115                 1120                 1125             


Phe Ala  Gly Val Ala Met Thr  Asn Leu Pro Gly Ile  Leu Val Leu 
    1130                 1135                 1140             


Gly Leu  Ala Lys Ala Gln Leu  Val Gln Ile Phe Phe  Phe Arg Leu 
    1145                 1150                 1155             


Asn Leu  Leu Val Thr Leu Leu  Gly Leu Ala His Gly  Leu Val Phe 
    1160                 1165                 1170             


Leu Pro  Val Val Leu Ser Tyr  Val Gly Pro Asp Val  Asn His Ala 
    1175                 1180                 1185             


Leu Val  Leu Gln Gln Lys Arg  Glu Glu Glu Ala Val  Ala Ala Arg 
    1190                 1195                 1200             


Glu Ala  Pro Cys Pro Lys His  Pro Ser Glu Thr Cys  Thr Asp Asn 
    1205                 1210                 1215             


Ser Ile  Tyr Val Asn Gln Gly  Phe Glu 
    1220                 1225         


<210>  20
<211>  1270
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NPC1L1

<400>  20

Ile Leu Trp Ala Val Cys His Leu Lys Val Asp Gly Val Thr Thr Ile 
1               5                   10                  15      


His Gln Lys Gly Tyr Cys Ser Met Tyr Glu Glu Cys Gly Leu Asn Pro 
            20                  25                  30          


Glu Val Ser Gly Ser Leu Ile Lys Pro Leu Ile Pro Cys Glu Ser Asn 
        35                  40                  45              


Thr Glu Ala Lys Lys Val Asn Glu Val His Met Thr Phe Leu Lys Asp 
    50                  55                  60                  


Val Cys Pro Gln Met Val Thr Gly Glu Asp Ser Thr Tyr Val Cys Cys 
65                  70                  75                  80  


Ser Ile Lys Gln Leu Gln Ser Ile Lys Ala Ser Phe Ala Leu Ser Lys 
                85                  90                  95      


Val Ile Leu Ser Arg Cys Pro Ser Cys Ala Glu Asn Phe Ala Asn Ile 
            100                 105                 110         


Tyr Cys Gln Asn Ile Cys Ser Pro Asp Gln Ser Thr Phe Thr Asn Val 
        115                 120                 125             


Thr Arg Thr His Thr Met Asn Val Ser Gly Asn Ile Glu Gln Gly Val 
    130                 135                 140                 


Leu Glu Tyr Asp Cys Tyr Phe Arg Arg Gln Phe Ala Asp Glu Ser Phe 
145                 150                 155                 160 


Asn Ser Cys Lys Asn Val Arg Leu Pro Ala Thr Gly Gly Tyr Ala Ile 
                165                 170                 175     


Ala Ala Met Cys Gly Lys Tyr Gly Ala Glu Leu Cys Thr Ser Gln Arg 
            180                 185                 190         


Trp Leu Asp Phe Gln Gly Asp Thr Ser Asn Gly Leu Ala Pro Leu Asp 
        195                 200                 205             


Ile Asn Phe Asn Leu Met Asp Ser Asn Thr Glu Ile Gly His Gly Ile 
    210                 215                 220                 


Val Pro His Tyr Ser Arg Val Trp Ser Cys Ser Glu Gly Pro Gly Asn 
225                 230                 235                 240 


Ser Thr Asp Asn Val Cys Thr Cys Gln Asp Cys Lys Glu Ser Cys Pro 
                245                 250                 255     


Lys Ile Pro Ala Pro Glu Pro Leu Pro Asp Pro Phe Gln Ile Gly His 
            260                 265                 270         


Leu Asp Gly Phe Leu Val Val Ser Leu Ala Val Phe Ala Ala Leu Leu 
        275                 280                 285             


Leu Leu Phe Leu Leu Phe Phe Val Ile Ser Cys Cys Ile Asn Ala Lys 
    290                 295                 300                 


Lys Glu Lys Lys Glu Pro Lys Glu Ile Pro Arg Val Thr Arg Ala Asp 
305                 310                 315                 320 


Leu Asn Cys Ser Glu Lys Leu Ser Leu Lys Thr Asn Lys Ala Leu Ser 
                325                 330                 335     


Arg Ser Phe Arg Trp Trp Gly Thr Leu Val Ala Ser Tyr Pro Lys Thr 
            340                 345                 350         


Val Ile Gly Val Ser Leu Ile Val Val Ile Ile Leu Ser Ala Gly Ile 
        355                 360                 365             


Ile Leu Ile Arg Leu Thr Thr Asp Pro Val Glu Leu Trp Ser Ala Val 
    370                 375                 380                 


Asn Ser Gln Ala Arg Gln Glu Lys Asp Phe His Asp Ala Asn Phe Gly 
385                 390                 395                 400 


Pro Phe Phe Arg Thr Asn Gln Leu Ile Ile Thr Ala Lys Asn Arg Val 
                405                 410                 415     


Ser Tyr Pro Tyr Asp Ser Leu Phe Phe Gly Lys Leu Asn Phe Ser Gly 
            420                 425                 430         


Ile Leu Ser Pro Asp Thr Ile Met Glu Met Leu Asp Leu Gln Glu Lys 
        435                 440                 445             


Val Gln Asn Ile Val Val Trp Ser Glu Lys His Gln Lys Asn Val Thr 
    450                 455                 460                 


Leu Lys Asp Ile Cys Tyr Ala Pro Leu Asn Pro Ser Asn Pro Thr Pro 
465                 470                 475                 480 


Thr Asp Cys Cys Val Asn Ser Leu Met Gln Tyr Phe Gln Asn Asp Arg 
                485                 490                 495     


Ala Lys Phe Asp Leu Glu Val Thr Gln Thr Met Ser Gly Gln Thr Gly 
            500                 505                 510         


Thr Val Gly Trp Arg Asp His Phe Met Tyr Cys Val Asn Ser Pro Leu 
        515                 520                 525             


Ser Phe Lys Asp Ile Thr Asp Leu Glu Leu Ser Cys Met Ala Asp Tyr 
    530                 535                 540                 


Gly Ala Pro Val Phe Pro Phe Leu Ala Val Gly Gly Tyr Lys Asp Gly 
545                 550                 555                 560 


Gln Phe Ser Glu Ala Glu Ala Leu Ile Leu Thr Phe Ser Leu Asn Asn 
                565                 570                 575     


Tyr Pro Arg Thr Asp Pro Arg Phe Asp Phe Val Leu Leu Trp Glu Lys 
            580                 585                 590         


Arg Tyr Leu Glu Ile Ile Lys Glu Tyr Lys Glu Asn Pro Lys Ser Asn 
        595                 600                 605             


Leu Thr Phe Gly Tyr Met Ala Glu Arg Ser Leu Glu Asp Glu Ile Asn 
    610                 615                 620                 


Arg Thr Thr Thr Glu Asp Ile Pro Ile Phe Thr Ile Ser Tyr Leu Val 
625                 630                 635                 640 


Ile Phe Ile Tyr Ile Ala Leu Ala Leu Gly Glu Tyr Ser Ser Cys Arg 
                645                 650                 655     


Arg Val Leu Val Asp Ser Lys Val Thr Leu Gly Leu Gly Gly Ile Leu 
            660                 665                 670         


Val Val Leu Gly Ala Val Leu Ser Ser Ile Gly Phe Tyr Ser Tyr Ile 
        675                 680                 685             


Gly Ile Pro Ser Thr Leu Ile Ile Val Glu Val Val Pro Phe Leu Val 
    690                 695                 700                 


Leu Ala Val Gly Ala Asp Asn Ile Phe Ile Phe Val Leu Glu Leu Gln 
705                 710                 715                 720 


Arg Asp Asp Arg Arg Pro Gly Glu Arg Arg Glu Glu Gln Ile Gly Arg 
                725                 730                 735     


Val Leu Gly Asn Val Ala Pro Ser Met Leu Leu Cys Ser Val Ser Glu 
            740                 745                 750         


Ser Leu Cys Phe Phe Leu Gly Ala Leu Thr Lys Met Pro Ala Val Arg 
        755                 760                 765             


Thr Phe Ala Leu Asn Ala Ala Leu Ala Ile Leu Leu Asp Phe Val Leu 
    770                 775                 780                 


Gln Ile Ser Met Phe Val Ala Leu Val Ser Leu Asp Ser Lys Arg Gln 
785                 790                 795                 800 


Glu Ala Ser Arg Tyr Asp Ile Cys Cys Cys Ile Lys Thr Lys Thr Glu 
                805                 810                 815     


Lys Pro Lys Lys Lys Ser Lys Gly Leu Leu Val Pro Phe Met Lys Lys 
            820                 825                 830         


Val Tyr Ser Pro Ile Leu Leu Asn Pro Val Ser Arg Phe Ile Val Met 
        835                 840                 845             


Val Val Phe Ile Phe Met Phe Cys Ala Gly Leu Tyr Phe Met Met His 
    850                 855                 860                 


Thr Arg Val Gly Leu Asn Gln Glu Leu Ser Val Pro Leu Asp Ser Tyr 
865                 870                 875                 880 


Val Leu Asp Tyr Phe Asp Tyr Leu Asn Arg Tyr Leu Glu Val Gly Val 
                885                 890                 895     


Pro Thr Tyr Phe Val Thr Thr Thr Gly Tyr Asn Phe Ser Ser Leu Asp 
            900                 905                 910         


Gly Ile Asn Gly Ile Cys Ser Ser Val Gly Cys Asp Gly Asp Ser Leu 
        915                 920                 925             


Thr Gln Lys Ile Gln Tyr Ala Thr Glu Tyr Pro Asp Arg Ser Tyr Leu 
    930                 935                 940                 


Ala Ile Pro Ala Ser Ser Trp Val Asp Asp Phe Ile Asp Trp Leu Asn 
945                 950                 955                 960 


Pro Thr Ser Asp Cys Cys Arg Leu Leu Leu Asn Gly Thr Phe Cys Ser 
                965                 970                 975     


Ser Thr Ser Ala Leu Pro Gly Cys Leu Arg Lys Cys Met Pro Ser Thr 
            980                 985                 990         


Thr Gly Ala Leu Arg Pro Asn Leu  Glu Gln Phe Asn Thr  Tyr Leu Pro 
        995                 1000                 1005             


Val Phe  Leu Asn Asp Tyr Pro  Asn Leu Lys Cys Pro  Lys Gly Gly 
    1010                 1015                 1020             


Leu Gly  Ala Tyr Asp Asn Ser  Val Lys Phe Asn Asp  Gln Gly Glu 
    1025                 1030                 1035             


Ile Ala  Ala Thr Arg Phe Met  Ala Phe His Thr Pro  Leu Lys Asn 
    1040                 1045                 1050             


Ser Gln  Glu Tyr Thr Ala Ala  Leu Lys Thr Ala Arg  Glu Leu Ala 
    1055                 1060                 1065             


Ala Asn  Ile Thr Glu Ser Leu  Arg Lys Val Pro Gly  Thr Asp Pro 
    1070                 1075                 1080             


Asn Phe  Arg Val Phe Pro Tyr  Thr Ile Thr Tyr Val  Phe Tyr Glu 
    1085                 1090                 1095             


Gln Tyr  Met Thr Ile Val Gln  Glu Gly Leu Phe Thr  Leu Ala Leu 
    1100                 1105                 1110             


Cys Leu  Ile Pro Thr Phe Ala  Val Cys Cys Ile Leu  Leu Gly Met 
    1115                 1120                 1125             


Asp Leu  Arg Ser Gly Phe Ile  Asn Leu Ile Thr Ile  Ile Met Ile 
    1130                 1135                 1140             


Leu Ile  Asp Thr Met Gly Val  Met Thr Leu Trp Gly  Ile Asp Tyr 
    1145                 1150                 1155             


Asn Ala  Ile Ser Leu Ile Asn  Leu Val Thr Ala Val  Gly Ile Ser 
    1160                 1165                 1170             


Val Glu  Phe Val Ser His Leu  Thr Arg Ser Phe Ala  Ile Ser Ser 
    1175                 1180                 1185             


Lys Pro  Asn Lys Val Glu Arg  Ala Lys Glu Ala Thr  Ile Asn Met 
    1190                 1195                 1200             


Gly Ser  Ala Val Phe Ala Gly  Val Ala Met Thr Asn  Leu Pro Gly 
    1205                 1210                 1215             


Ile Ile  Val Leu Ala Phe Ala  Lys Ala Gln Leu Ile  Gln Ile Phe 
    1220                 1225                 1230             


Phe Phe  Arg Leu Asn Leu Thr  Ile Thr Leu Leu Gly  Leu Val His 
    1235                 1240                 1245             


Gly Leu  Ile Phe Leu Pro Val  Val Leu Ser Tyr Phe  Gly Pro Gly 
    1250                 1255                 1260             


Ile Asn  Arg Ala Val Leu Leu  
    1265                 1270 


<210>  21
<211>  1278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NPC1L1

<400>  21

His Gln Pro Gly Phe Cys Ala Phe Tyr Asp Glu Cys Gly Arg Asn Pro 
1               5                   10                  15      


Ser Leu Gly Asp Thr Leu Ile Pro Pro Ile Val Pro Cys Leu Asn Tyr 
            20                  25                  30          


Ser Pro Ala Arg His Leu Thr Gly Glu His Tyr Arg Arg Phe Lys Glu 
        35                  40                  45              


Val Cys Pro Met Leu Asp Gln Gly Glu Glu Asn Thr Tyr Gly Cys Cys 
    50                  55                  60                  


Ser Leu Gly Gln Leu Thr Ser Leu Glu Arg Ser Leu Ser Leu Ser Lys 
65                  70                  75                  80  


Gly Val Leu Asn Arg Cys Pro Ser Cys Ala Glu Asn Phe Ala His Met 
                85                  90                  95      


His Cys Met Ala Thr Cys Ser Pro Asn Gln Thr Val Ala Val Lys Val 
            100                 105                 110         


Thr Lys Val Met Asn Val Thr Tyr Leu Gly Gln Pro Lys Asp Ala Val 
        115                 120                 125             


Val Ala Tyr Ser Ala Tyr Ile Asn Asn Gln Phe Ala Glu Thr Ala Phe 
    130                 135                 140                 


Gln Ser Cys Lys Asn Val Arg Leu Pro Ala Thr Gly Gly Phe Ala Ile 
145                 150                 155                 160 


Ser Thr Met Cys Gly Arg Tyr Gly Ala Thr Leu Cys Thr Pro Gln Arg 
                165                 170                 175     


Trp Tyr Asp Phe Gln Gly Asp Ser Ser Asn Gly Leu Ala Pro Leu Asp 
            180                 185                 190         


Ile Asp Phe His Leu Ile Lys Gly His Asp Thr Thr Gly Leu Pro Glu 
        195                 200                 205             


Gly Val Ile Pro Tyr Asn Gly Gln Ala Leu Lys Cys Asn Glu Val Thr 
    210                 215                 220                 


Pro Ser Gly Ser Glu Pro Cys Ser Cys Gln Asp Cys Lys Asp Ser Cys 
225                 230                 235                 240 


Pro Ser Leu Pro Pro Pro Pro Pro Pro Pro Ala Pro Phe Arg Leu Ala 
                245                 250                 255     


Gly Met Asp Gly Phe Leu Leu Ile Ser Ile Ile Leu Ser Cys Val Leu 
            260                 265                 270         


Ile Cys Ala Phe Leu Leu Tyr Val Phe Val Ser Phe Arg Met Ser Ser 
        275                 280                 285             


Lys Lys Lys Lys Asp Gln Gly Gly Gly Lys Arg Lys Asp Lys Thr Lys 
    290                 295                 300                 


Asp Ile Phe Gln Arg Lys Ile His Pro Ser Glu Val Thr Cys Ala Asn 
305                 310                 315                 320 


Lys Thr Ser Leu Val Ala Gln Asp Phe Leu Ser Ser Leu Phe Gln Arg 
                325                 330                 335     


Trp Gly Thr Leu Met Ala Ser Tyr Pro Leu Thr Val Leu Leu Phe Ser 
            340                 345                 350         


Ala Val Val Val Ala Val Leu Ser Val Gly Leu Lys Ser Ile Glu Leu 
        355                 360                 365             


Thr Thr Asp Pro Val Glu Leu Trp Ser Ala Pro Asn Ser Gln Ala Arg 
    370                 375                 380                 


Gln Glu Lys Asp Phe His Asp Lys His Phe Gly Pro Phe Phe Arg Thr 
385                 390                 395                 400 


Asn Gln Leu Ile Leu Thr Ala Pro Asp Lys Lys Gly Ser Ile Tyr Asp 
                405                 410                 415     


Ser Leu Leu Phe Gly Lys Gln Asn Phe Ser Gly Ile Val Ser Lys Asp 
            420                 425                 430         


Leu Ile Ile Gln Leu Leu Glu Leu Gln Gln Lys Ile Gln Gln Ile Glu 
        435                 440                 445             


Phe Trp Ser Glu Asp Leu Asn Arg Thr Ala Ser Leu Lys Asp Val Cys 
    450                 455                 460                 


Tyr Ala Pro Leu Asn Pro Ser Asn Pro Ser Leu Thr Asp Cys Ala Val 
465                 470                 475                 480 


Asn Ser Leu Pro Gln Tyr Phe Gln Asn Ser Leu Glu Asn Ile Asn Ala 
                485                 490                 495     


Lys Ala Asn Met Thr Glu Leu Gly Val Thr Lys Glu Val Asp Trp Arg 
            500                 505                 510         


Asp His Leu Ile Tyr Cys Leu Asn Ser Pro Leu Ser Phe Lys Asp Ile 
        515                 520                 525             


Thr Ala Leu Gly Met Ser Cys Met Ala Asp Tyr Gly Ala Pro Val Phe 
    530                 535                 540                 


Thr Phe Leu Ala Val Gly Gly Tyr Glu Asp Asp Asp Tyr Thr Asn Ala 
545                 550                 555                 560 


Glu Ala Leu Ile Leu Thr Phe Ser Leu Asn Asn Phe Pro Arg Glu Asp 
                565                 570                 575     


Pro Lys Phe Lys Val Ala Leu Gln Trp Glu Lys Glu Phe Leu Lys Ile 
            580                 585                 590         


Val Gln Asp Tyr Gln Lys Asn Pro Ala Thr Asn Phe Thr Phe Ala Tyr 
        595                 600                 605             


Met Ala Glu Arg Ser Leu Glu Asp Glu Ile Asn Arg Thr Thr Ala Glu 
    610                 615                 620                 


Asp Ile Pro Ile Phe Met Ile Ser Tyr Leu Val Ile Phe Val Tyr Ile 
625                 630                 635                 640 


Ala Val Ala Leu Gly Glu Tyr Ser Ser Cys Lys Arg Leu Leu Val Asp 
                645                 650                 655     


Ser Lys Phe Leu Val Gly Leu Gly Gly Ile Leu Val Val Gly Cys Ser 
            660                 665                 670         


Val Leu Ala Ser Met Gly Phe Tyr Ser Trp Ile Gly Ile Pro Ser Ser 
        675                 680                 685             


Leu Val Ile Leu Gln Val Val Pro Phe Leu Val Leu Ala Val Gly Ala 
    690                 695                 700                 


Asp Asn Ile Phe Ile Phe Val Leu Glu Tyr Gln Arg Asp Val Arg Arg 
705                 710                 715                 720 


Ala Gly Glu Thr Arg Glu Gln His Ile Gly Arg Val Leu Gly His Val 
                725                 730                 735     


Ala Pro Ser Met Leu Leu Cys Ser Leu Ser Glu Ser Val Cys Phe Phe 
            740                 745                 750         


Leu Gly Ala Leu Ser Thr Met Pro Ala Val Arg Ser Phe Ala Leu Tyr 
        755                 760                 765             


Ala Ala Leu Ala Val Leu Met Asp Phe Ala Leu Gln Met Thr Ala Phe 
    770                 775                 780                 


Val Ala Leu Leu Ser Leu Asp Ala Arg Arg Gln Val Lys Asn Arg Cys 
785                 790                 795                 800 


Glu Leu Phe Cys Cys Val Ser Val Ser Ala Lys His Ser Asn Lys Pro 
                805                 810                 815     


Asn Glu Gly Phe Leu Leu Pro Leu Met Arg Lys Tyr Tyr Ala Pro Ala 
            820                 825                 830         


Leu Leu Asn Arg Tyr Thr Arg Ile Phe Val Met Val Val Phe Ile Leu 
        835                 840                 845             


Met Phe Cys Ala Ser Ile Phe Leu Met Leu His Val Lys Val Gly Leu 
    850                 855                 860                 


Asp Gln Glu Leu Ala Met Pro Thr Asp Ser Tyr Met Leu Arg Tyr Phe 
865                 870                 875                 880 


Ser Tyr Leu His Lys Tyr Phe Glu Val Gly Val Pro Val Tyr Phe Val 
                885                 890                 895     


Thr Lys Arg Gly Phe Asn Phe Thr Ser Glu Glu Gly Met Asn Ala Val 
            900                 905                 910         


Cys Ser Ser Val Gly Cys Asp Gln Phe Ser Leu Thr Gln Lys Ile Gln 
        915                 920                 925             


Tyr Ala Thr Asn Phe Pro Asp Leu Ser Tyr Ile Ala Ile Pro Ala Asn 
    930                 935                 940                 


Ser Trp Val Asp Asp Phe Ile Asp Trp Leu Asn Pro Gly Ser Arg Cys 
945                 950                 955                 960 


Cys Arg Leu Tyr Thr Leu Gly Pro Asn Gln Gly Glu Phe Cys Pro Ala 
                965                 970                 975     


Asn Ile Ser Ser Leu Leu Cys Gly Arg Lys Cys Met Asn Ala Ser Ala 
            980                 985                 990         


Thr Ser Ala Ile Arg Pro Ser Val  Glu Leu Phe Asn Arg  Phe Leu Pro 
        995                 1000                 1005             


Asp Phe  Leu Gly Asn Arg Pro  Asp Leu Gln Cys Ser  Lys Gly Gly 
    1010                 1015                 1020             


Leu Gly  Ala Tyr Asp Thr Ser  Val Val Ile Asp Gly  Gly Gly Glu 
    1025                 1030                 1035             


Ile Ile  Ala Ser Arg Phe Met  Ala Tyr His Thr Thr  Leu Thr Asn 
    1040                 1045                 1050             


Ser Gln  Glu Tyr Thr Ala Ala  Leu Gln Lys Gly Arg  Glu Leu Ala 
    1055                 1060                 1065             


Ser Ser  Ile Thr Glu Ala Met  Arg Lys Val Pro Gly  Thr Ser Pro 
    1070                 1075                 1080             


Asp Phe  Glu Val Phe Thr Tyr  Thr Leu Thr Asn Val  Phe Tyr Glu 
    1085                 1090                 1095             


Gln Tyr  Leu Thr Ile Val Pro  Glu Gly Leu Phe Asn  Ile Ser Met 
    1100                 1105                 1110             


Cys Leu  Leu Pro Thr Phe Val  Val Cys Cys Leu Leu  Leu Gly Leu 
    1115                 1120                 1125             


Asp Leu  Leu Ser Gly Leu Leu  Asn Leu Ile Thr Ile  Ile Met Ile 
    1130                 1135                 1140             


Val Val  Asp Thr Val Gly Val  Met Thr Leu Trp Ser  Ile Asp Tyr 
    1145                 1150                 1155             


Asn Ala  Val Ser Leu Ile Asn  Leu Val Thr Ala Val  Gly Ile Ser 
    1160                 1165                 1170             


Val Glu  Phe Val Ser His Met  Thr Arg Ser Phe Ala  Leu Ser Thr 
    1175                 1180                 1185             


Lys Pro  Thr Arg Val Glu Arg  Ala Ala Glu Ala Thr  Ala Asn Met 
    1190                 1195                 1200             


Gly Ser  Ala Val Phe Ala Gly  Val Ala Met Thr Asn  Leu Pro Gly 
    1205                 1210                 1215             


Ile Leu  Val Leu Ala Phe Ala  Lys Ala Gln Leu Ile  Gln Ile Phe 
    1220                 1225                 1230             


Phe Phe  Arg Leu Asn Leu Val  Ile Thr Leu Leu Gly  Leu Ala His 
    1235                 1240                 1245             


Gly Leu  Ile Phe Leu Pro Val  Leu Leu Thr Tyr Phe  Gly Pro Gly 
    1250                 1255                 1260             


Leu Asn  Lys Ala Ile Leu Leu  Gln Ala Gln Arg Ala  Asn Gln Ser 
    1265                 1270                 1275             


<210>  22
<211>  1032
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NPC1L1

<400>  22

His Glu Pro Gly Phe Cys Ala Met Tyr Glu Asp Cys Gly Leu Asn Pro 
1               5                   10                  15      


Ala Val Glu Gly Ala Leu Ile Pro Pro Arg Val Pro Cys Lys Asp Tyr 
            20                  25                  30          


Arg Arg Ala Val Asn Val Thr Gly Asp His Tyr Glu Leu Leu Lys Ser 
        35                  40                  45              


Val Cys Pro Met Leu Ala Asn Gly Asp Gly Gln Thr Leu Ala Cys Cys 
    50                  55                  60                  


Ser Ile Arg Gln Leu Thr Ala Leu Gln Asn Ser Leu Ser Leu Ser Lys 
65                  70                  75                  80  


Ala Val Leu Ile Arg Cys Pro Ser Cys Ala Glu Asn Phe Ala Tyr Leu 
                85                  90                  95      


His Cys Ala Thr Thr Cys Ser Pro Asp Gln Ser Gln Ile Leu Lys Ile 
            100                 105                 110         


Thr Lys Thr Ala Asn Ile Thr Gln Pro Thr Gly Ile Val Lys Glu Ala 
        115                 120                 125             


Val Val Gly Tyr Ala Ala Tyr Ile Ser Thr Asn Phe Ser Asp Ala Ser 
    130                 135                 140                 


Phe Asp Ser Cys Lys Asn Val Arg Ile Pro Ser Thr Gly Gly Phe Ala 
145                 150                 155                 160 


Ile Ala Thr Met Cys Gly Arg Tyr Gly Ala Ala Leu Cys Thr Pro Gln 
                165                 170                 175     


Arg Trp Tyr Asp Phe Gln Gly Asp Ser Ser Asn Gly Leu Ala Pro Leu 
            180                 185                 190         


Asp Ile Asp Phe Lys Leu Leu Gln Asp Ser Glu Thr Ser Glu Val Pro 
        195                 200                 205             


Ala Gly Ala Phe Ala Phe Ala Gly Gln Ala Leu Lys Cys Asn Glu Val 
    210                 215                 220                 


Thr Pro Ser Gly Gly Glu Ala Cys Ser Cys Gln Asp Cys Leu Gln Ser 
225                 230                 235                 240 


Cys Pro Val Val Pro Glu Pro Pro Pro Leu Pro Lys Pro Phe Met Ile 
                245                 250                 255     


Gly Asn Leu Asn Gly Val Leu Val Ile Cys Leu Thr Val Phe Ser Cys 
            260                 265                 270         


Phe Leu Phe Leu Phe Leu Cys Tyr Val Ile Thr Glu Cys Thr Thr Tyr 
        275                 280                 285             


Tyr Met Lys Ser Arg Lys Gly Arg Lys Gly Lys Ser Thr Lys Asp Gln 
    290                 295                 300                 


Asn Ala Asn Glu Ile Lys Phe Lys Ile Ser Pro Lys Asp Val Ser Cys 
305                 310                 315                 320 


Ser Asp Lys Ala Ser Leu Ala Thr Gln Glu Phe Leu Gly Ser Leu Phe 
                325                 330                 335     


Gln Ser Trp Gly Thr Leu Met Ala Arg Tyr Pro Tyr Ile Val Leu Pro 
            340                 345                 350         


Val Cys Leu Val Ile Val Leu Val Phe Ala Val Gly Ile Lys Asp Ile 
        355                 360                 365             


Glu Leu Thr Thr Asp Pro Val Gln Leu Trp Ser Ala Pro Glu Ser Arg 
    370                 375                 380                 


Ala Met Arg Glu Lys Ala Phe His Asp Ala His Phe Asp Pro Phe Tyr 
385                 390                 395                 400 


Arg Thr Asn Gln Leu Ile Leu Thr Ala Pro Gly Arg Pro Ser His Phe 
                405                 410                 415     


Tyr Asp Ser Leu Leu Phe Gly Lys Gln Asn Phe Ser Gly Ile Ile Ser 
            420                 425                 430         


Lys Asp Leu Ile Ile Glu Leu Leu Lys Leu Gln Gln Ser Ile Gln Ala 
        435                 440                 445             


Ile Glu Phe Trp Ser Asp Glu Leu Asn Ser Thr Ala Ser Leu Lys Asp 
    450                 455                 460                 


Val Cys Tyr Ala Pro Leu Asn Pro Gly Asn Pro Ser Leu Thr Asp Cys 
465                 470                 475                 480 


Ala Val Asn Ser Leu Pro Gln Tyr Phe Gln Asn Ser Ile Asp Asn Leu 
                485                 490                 495     


Asn Ala Lys Val Asn Met Thr Glu Leu Gly Val Thr Lys Glu Val Asp 
            500                 505                 510         


Trp Arg Asp His Phe Ile Tyr Cys Val Asn Ser Pro Leu Ser Phe Lys 
        515                 520                 525             


Asp Ile Thr Ala Leu Gly Met Ser Cys Met Ser Asp Tyr Gly Gly Pro 
    530                 535                 540                 


Val Phe Pro Phe Leu Ala Val Gly Gly Tyr Asp Asn Glu Gln Tyr Thr 
545                 550                 555                 560 


Thr Ala Glu Ala Leu Ile Leu Thr Phe Ser Leu Asn Asn Tyr Ala Arg 
                565                 570                 575     


Thr Asp Val Lys Phe Lys Val Val Glu Glu Trp Glu Asn Arg Phe Leu 
            580                 585                 590         


Lys Ile Val Gln Glu Tyr Gln Lys Asn Ser Thr Thr Asn Phe Thr Phe 
        595                 600                 605             


Ala Tyr Met Ala Glu Arg Ser Leu Glu Asp Glu Ile Asn Arg Thr Thr 
    610                 615                 620                 


Ala Glu Asp Ile Pro Ile Phe Met Ile Ser Tyr Ala Val Ile Phe Leu 
625                 630                 635                 640 


Tyr Ile Ala Val Ala Leu Gly Glu Tyr Ser Ser Cys Lys Arg Ile Leu 
                645                 650                 655     


Val Asp Ser Lys Phe Leu Val Gly Leu Gly Gly Ile Leu Val Val Gly 
            660                 665                 670         


Cys Ser Val Ile Ala Ser Met Gly Phe Tyr Ala Trp Ile Gly Val Ala 
        675                 680                 685             


Ser Ser Leu Val Ile Leu Gln Val Val Pro Phe Leu Val Leu Ala Val 
    690                 695                 700                 


Gly Ala Asp Asn Ile Phe Ile Phe Val Leu Glu Tyr Gln Arg Asp Leu 
705                 710                 715                 720 


Arg Arg Pro Gly Glu Gln Arg Glu Glu Gln Ile Gly Arg Val Leu Gly 
                725                 730                 735     


Asn Val Ala Pro Ser Met Leu Leu Cys Ser Leu Ser Glu Ser Val Cys 
            740                 745                 750         


Phe Phe Leu Gly Ala Leu Ser Thr Met Pro Ala Val Lys Ser Phe Ala 
        755                 760                 765             


Leu Tyr Ala Ala Leu Ala Val Leu Met Asp Phe Val Leu Gln Met Thr 
    770                 775                 780                 


Ala Phe Val Ala Leu Leu Ser Leu Asp Ala Arg Arg Gln Asp Ser Asn 
785                 790                 795                 800 


Arg Cys Glu Ile Ala Cys Cys Val Thr Val Asp Thr Pro Arg Pro Gln 
                805                 810                 815     


Lys Pro Asn Glu Gly Ile Leu Leu Pro Leu Met Arg Lys Tyr Tyr Ala 
            820                 825                 830         


Pro Phe Leu Leu Asn Ser Phe Ser Arg Val Val Val Met Val Leu Phe 
        835                 840                 845             


Leu Val Met Phe Phe Ser Ser Val Phe Leu Met Phe His Val Lys Val 
    850                 855                 860                 


Gly Leu Asn Gln Glu Leu Ala Met Pro Thr Asp Ser Tyr Met Leu Asp 
865                 870                 875                 880 


Tyr Phe Ala Tyr Leu Phe Lys Tyr Phe Glu Val Gly Val Pro Thr Tyr 
                885                 890                 895     


Phe Ile Thr Thr Lys Gly Phe Asn Phe Asn Ser Glu Glu Gly Ile Asn 
            900                 905                 910         


Ala Val Cys Ser Ser Val Gly Cys Asp Gln Phe Ser Met Thr Gln Lys 
        915                 920                 925             


Ile Arg Tyr Ala Thr Glu Tyr Pro Glu Arg Ser Tyr Leu Ala Ile Pro 
    930                 935                 940                 


Ala Ser Ser Trp Val Asp Asp Tyr Ile Asp Trp Leu Asn Pro Gly Ser 
945                 950                 955                 960 


Arg Cys Cys Arg Leu Tyr Thr Phe Gly Pro Asn Lys Asp Glu Phe Cys 
                965                 970                 975     


Pro Ala Asn Glu Thr Gly Val Cys Leu Lys Lys Cys Met Gly Lys Pro 
            980                 985                 990         


Glu Asn Gly Val Leu Arg Pro Asp  Val Ser Glu Phe Asn  Arg Phe Leu 
        995                 1000                 1005             


Pro Asp  Phe Leu Ser Asn Arg  Pro Asp Leu Gln Cys  Pro Lys Gly 
    1010                 1015                 1020             


Gly Leu  Gly Ala Tyr Asp Lys  Ala Val 
    1025                 1030         


<210>  23
<211>  1102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NPC1L1

<400>  23

Phe Thr Pro Ile His Arg Ala Gly Tyr Cys Ser Phe Tyr Glu Glu Cys 
1               5                   10                  15      


Gly Arg Asn Pro Glu Ile Ser Ser Ser Leu Ile Pro Pro Ile Val Pro 
            20                  25                  30          


Cys Leu Asn Asn Ser Pro Ala Arg Ala Leu Glu Gly Glu His Leu Gln 
        35                  40                  45              


Leu Leu Gln Glu Val Cys Pro Arg Leu Asp Gln Gly Pro Gly Ala Thr 
    50                  55                  60                  


Phe Ala Cys Cys Ser Leu Lys Gln Leu Arg Ser Leu Lys Arg Ser Ile 
65                  70                  75                  80  


Ala Leu Ser Lys Val Ile Leu Thr Arg Cys Pro Ala Cys Ala Glu Asn 
                85                  90                  95      


Phe Ala Ser Leu His Cys Gln Asn Thr Cys Ser Pro Asn Gln Ser Leu 
            100                 105                 110         


Phe Ile Asn Val Thr Arg Val His Thr Asp Ser Ser Ala Val Leu Ala 
        115                 120                 125             


Tyr Gln Ser Phe Tyr Thr Arg Arg Tyr Ala Glu Glu Ala Tyr Ser Ser 
    130                 135                 140                 


Cys Ser Glu Val Arg Ile Pro Ala Thr Gly Gly Leu Ala Val Gly Ala 
145                 150                 155                 160 


Met Cys Gly Val Tyr Gly Ala Glu Leu Cys Asn Ala Gln Arg Trp Leu 
                165                 170                 175     


Asp Phe Gln Gly Asn Pro Ser Asn Gly Leu Ala Pro Leu Gln Val Asp 
            180                 185                 190         


Phe His Leu Leu Asp Pro Gly Gln Ala Pro Gly Ala Gly Leu Gln Pro 
        195                 200                 205             


Leu Asp Thr Asp Leu Trp Gln Cys His Leu Ser Tyr Gly Asp Thr Met 
    210                 215                 220                 


Glu Ala Cys Ser Cys Gln Asp Cys Ala Thr Ala Cys Pro Val Leu Pro 
225                 230                 235                 240 


Ser Leu Asp Pro Glu Asn Ser Gly Phe Arg Leu Gly Ile Leu Leu Ile 
                245                 250                 255     


Leu Ala Leu Leu Leu Ala Ala Ala Leu Ile Cys Gly Ala Ala Thr Ala 
            260                 265                 270         


Val Phe Arg Cys Tyr Arg Arg Thr Lys Ala Lys Gln Ala Gly Gln Pro 
        275                 280                 285             


Glu Val Ser Cys Lys Asp Arg Leu Ser Leu Ser Thr His Thr Ala Leu 
    290                 295                 300                 


Gly Arg Leu Phe Gln Arg Trp Gly Thr Trp Ala Ala Ala Trp Pro Leu 
305                 310                 315                 320 


Thr Val Leu Gly Leu Ala Ala Val Ala Val Val Ala Leu Ala Gly Gly 
                325                 330                 335     


Leu Ala Tyr Leu Glu Leu Thr Thr Asp Pro Val Glu Leu Trp Ser Ala 
            340                 345                 350         


Pro Asp Ser Gln Ala Arg Gln Glu Lys Ala Phe His Asp Arg His Phe 
        355                 360                 365             


Gly Pro Phe Phe Arg Thr Asn Gln Val Ile Leu Thr Ala Pro Gly Arg 
    370                 375                 380                 


Ala Ser Tyr Arg Tyr Asp Ser Leu Leu Leu Gly Pro Lys Asn Phe Ser 
385                 390                 395                 400 


Gly Leu Leu Ala Pro Asp Val Leu Leu Gln Leu Leu Asp Leu Gln Glu 
                405                 410                 415     


Arg Leu Arg Asn Leu Gln Val Trp Ser Glu Pro Asp Gly Arg Asn Val 
            420                 425                 430         


Ser Leu Arg Asp Phe Cys Tyr Ala Pro Leu Asn Pro Arg Asn Ala Ser 
        435                 440                 445             


Leu Thr Asp Cys Ala Ile Asn Ser Leu Leu Gln Tyr Phe Gln Ser Asn 
    450                 455                 460                 


Arg Thr Arg Phe Leu Leu Thr Ala Asn Gln Thr Leu Ala Gly Gln Thr 
465                 470                 475                 480 


Ala Gln Val Asp Trp Arg Asp His Phe Leu Tyr Cys Ala Asn Ser Pro 
                485                 490                 495     


Leu Ser Phe Lys Asp Gly Thr Ala Leu Glu Leu Ser Cys Met Ala Asp 
            500                 505                 510         


Tyr Gly Ala Pro Val Phe Pro Phe Leu Ala Val Gly Gly Tyr Lys Gly 
        515                 520                 525             


Arg Asp Phe Ser Glu Ala Glu Ala Leu Ile Leu Thr Phe Ser Leu Asn 
    530                 535                 540                 


Asn Phe Pro Thr Asp Asp Pro Arg Leu Ala Gln Ala Gln Leu Trp Glu 
545                 550                 555                 560 


Ser Arg Phe Leu Glu Val Leu Arg Asp Phe Arg Gln Ser Thr Gln Gly 
                565                 570                 575     


Gln Leu Ser Val Thr Phe Met Ala Glu Glu Gly Gly Ala Met Thr Arg 
            580                 585                 590         


Met Pro Ala Val Arg Thr Phe Ala Leu Thr Ala Gly Leu Ala Val Ile 
        595                 600                 605             


Phe Asp Phe Leu Leu Gln Met Thr Ala Phe Val Ala Leu Leu Ser Leu 
    610                 615                 620                 


Asp Ala Arg Arg Gln Glu Ala Ser Arg Leu Asp Val Cys Cys Cys Val 
625                 630                 635                 640 


Gln Met Ala Asp Ala Pro Ala Arg Pro Arg Gly Glu Gly Leu Leu Leu 
                645                 650                 655     


Arg Phe Phe Arg Arg Phe Tyr Val Pro Phe Leu Leu His Arg Val Thr 
            660                 665                 670         


Arg Val Val Val Met Val Leu Phe Leu Leu Leu Phe Cys Val Gly Leu 
        675                 680                 685             


Phe Phe Met Val Phe Ile Asn Ile Gly Leu Asp Gln Glu Leu Ala Leu 
    690                 695                 700                 


Pro Arg Asp Ser Tyr Leu Leu Asp Tyr Phe Leu Tyr Leu Asn Arg Tyr 
705                 710                 715                 720 


Phe Glu Val Gly Val Pro Thr Tyr Phe Val Thr Thr Ala Gly Phe Asn 
                725                 730                 735     


Phe Ser Ser Thr Glu Ala Met Glu Ala Ile Cys Ser Ser Ala Gly Cys 
            740                 745                 750         


Gln Asn Asn Ser Leu Thr Gln Lys Ile Gln Phe Ala Thr Gln Val Ser 
        755                 760                 765             


Asn Leu Ser Tyr Leu Ala Ile Pro Ala Ser Ser Trp Val Asp Asp Phe 
    770                 775                 780                 


Ile Asp Trp Leu Asn Pro Leu Ser Glu Cys Cys Arg Ile His Ser Phe 
785                 790                 795                 800 


Gly Pro Asn Lys Asp Glu Phe Cys Pro Ser Thr Val Asn Ser Leu Ser 
                805                 810                 815     


Cys Thr Met Lys Lys Cys Met Lys Pro Thr Leu Gly Val Leu Arg Pro 
            820                 825                 830         


Thr Pro Glu Gln Phe Asn Lys Tyr Leu Pro Trp Phe Leu Asn Asp Tyr 
        835                 840                 845             


Pro Asn Leu Lys Cys Pro Lys Gly Gly Leu Gly Ala Tyr Asp Gly Ser 
    850                 855                 860                 


Val Lys Ile Gly Pro Gly Gly Glu Ile Leu Ala Ser Arg Phe Met Ala 
865                 870                 875                 880 


Tyr His Thr Pro Leu Lys Asn Ser Gln Glu Tyr Thr Gly Ala Leu Arg 
                885                 890                 895     


Val Ala Arg Lys Leu Ala Asp Asn Ile Thr Ala Thr Leu Arg Lys Val 
            900                 905                 910         


Pro Gly Thr Asp Pro Asn Phe Lys Val Phe Pro Tyr Thr Ile Thr Tyr 
        915                 920                 925             


Val Phe Tyr Glu Gln Tyr Leu Thr Ile Val Val Glu Gly Leu Phe Ile 
    930                 935                 940                 


Val Ala Leu Cys Leu Thr Pro Thr Phe Leu Val Cys Cys Phe Leu Leu 
945                 950                 955                 960 


Gly Met Asp Leu Arg Ser Gly Leu Leu Asn Leu Val Ser Ile Ile Met 
                965                 970                 975     


Ile Val Val Asp Thr Leu Gly Phe Met Thr Leu Trp Gly Ile Ser Tyr 
            980                 985                 990         


Asn Ala Val Ser Leu Ile Asn Leu  Val Thr Ala Val Gly  Ile Ser Val 
        995                 1000                 1005             


Glu Phe  Val Ser His Ile Thr  Arg Ser Phe Ala Ile  Ser Thr Arg 
    1010                 1015                 1020             


Pro Thr  Arg Leu Glu Arg Ala  Thr Glu Ala Thr Val  Val Met Gly 
    1025                 1030                 1035             


Ser Glu  Val Phe Ala Gly Val  Ala Met Thr Asn Leu  Pro Gly Ile 
    1040                 1045                 1050             


Ile Val  Leu Ala Phe Ala Asn  Ala Gln Leu Ile Gln  Ile Phe Phe 
    1055                 1060                 1065             


Phe Arg  Leu Asn Leu Leu Ile  Thr Ile Leu Gly Thr  Leu His Gly 
    1070                 1075                 1080             


Leu Val  Phe Leu Pro Val Ala  Leu Ser Tyr Leu Gly  Pro Gly Val 
    1085                 1090                 1095             


Asn Gln  Ala Leu 
    1100         


<210>  24
<211>  1317
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NPC1L1


<220>
<221>  misc_feature
<222>  (987)..(987)
<223>  Xaa can be any naturally occurring amino acid

<400>  24

Met Leu Arg Trp Ala Leu Phe Ala Leu Val Val Leu Arg Ala Arg Met 
1               5                   10                  15      


Asp Pro Leu Thr Glu Ile His Arg Ala Gly Tyr Cys Ser Phe Tyr Gly 
            20                  25                  30          


Ser Cys Asp Gln Asn Pro Glu Val Asn Gly Ser Gly Ile Thr Thr Pro 
        35                  40                  45              


Cys Leu Ser Asn Thr Pro Ala Arg Leu Ala His Gly Ser His Leu Ile 
    50                  55                  60                  


Leu Leu Lys Thr Ile Cys Pro Met Leu Phe Lys Gly Ala Ser Asn Thr 
65                  70                  75                  80  


His Ile Cys Cys Ser Glu Lys Gln Leu Glu Phe Leu Glu Ser Ser Phe 
                85                  90                  95      


Ser Leu Ser Arg Ala Leu Leu Ser Arg Cys Pro Ala Cys Met Glu Asn 
            100                 105                 110         


Phe Ala Asn Ile His Cys Gln Thr Ile Cys Ser Pro Asp Gln Ser Leu 
        115                 120                 125             


Phe Thr Asn Val Thr Arg Tyr Phe Asn Arg Thr Ile Val Gly Gly Leu 
    130                 135                 140                 


Gln Val Gly Val Leu Glu Phe Gln Cys Tyr Tyr Asn Gln His Phe Ala 
145                 150                 155                 160 


Glu Glu Thr Tyr Gln Ser Cys Lys Gly Val Arg Leu Pro Ala Thr Gly 
                165                 170                 175     


Asn Ser Val Ile Ser Thr Met Cys Gly Lys Tyr Gly Glu Ala Ala Cys 
            180                 185                 190         


Asn Thr Gln Arg Trp Leu Asp Phe Gln Gly Asp Pro Arg Asn Gly Met 
        195                 200                 205             


Ala Pro Leu Ala Ile Ser Phe Gln Leu Lys Pro Gly Asp Trp Ala Ala 
    210                 215                 220                 


Glu Glu Gly Asp Ile Leu Pro Leu Asn Gly Thr Pro Trp Lys Cys Ser 
225                 230                 235                 240 


Glu Ala Val Asn Asn Gly Thr Lys Cys Ser Cys Ser Asp Cys Glu Gln 
                245                 250                 255     


Ser Cys Pro Ala Ile Gln Pro Leu Pro Ser Glu Ser Gln Pro Phe Lys 
            260                 265                 270         


Val Gly Asn Met Asp Gly Val Leu Phe Ile Cys Ile Leu Leu Phe Ser 
        275                 280                 285             


Ile Leu Thr Leu Ser Phe Ile Ser Phe Leu Leu Trp Arg Arg Tyr Phe 
    290                 295                 300                 


Ser Ser Lys Lys Gln Lys Asp His Ser Ala Lys Asp Ala Ala Thr Lys 
305                 310                 315                 320 


Leu Thr Gly Ser Glu Lys Val Ser Gln Ala Met Tyr Gly Phe Phe Ile 
                325                 330                 335     


Arg Ala Phe Ser Ser Trp Gly Ile Leu Val Ala Ser Asn Pro Ile Thr 
            340                 345                 350         


Val Ile Ile Ile Ser Leu Ala Ile Val Val Gly Leu Ser Ser Gly Leu 
        355                 360                 365             


Ile Trp Met Gln Leu Thr Thr Asp Pro Leu Glu Leu Trp Ser Ser Pro 
    370                 375                 380                 


Asp Ser Gln Ala Arg Gln Glu Lys Ala Leu Tyr Glu Glu Asn Phe Gly 
385                 390                 395                 400 


Arg Phe Phe Arg Thr Ser Gln Val Ile Leu Thr Ala Lys Asn His Ser 
                405                 410                 415     


Ser His Ile Tyr Asp Ser Leu Phe Trp Gly Lys Lys Asn Phe Ser Gly 
            420                 425                 430         


Val Leu Ser Glu Lys Val Leu Leu Asp Leu Met Ser Leu Gln Val Lys 
        435                 440                 445             


Leu Arg Asp Met Glu Ile Trp Ser Glu Lys His His Arg Asn Ile Thr 
    450                 455                 460                 


Leu Lys Asp Ile Cys Tyr Ala Pro Leu Asn Pro Gln Asn Ala Ser Leu 
465                 470                 475                 480 


Ser Asp Cys Cys Val Asn Ser Leu Leu Gln Phe Phe Gln Asn Asn Arg 
                485                 490                 495     


Thr Ala Phe Leu Leu Thr Ala Asn Gln Thr Val Asp Gly Lys Thr Gly 
            500                 505                 510         


Thr Val Asp Trp Arg Asp His Phe Leu Tyr Cys Ile Asn Ser Pro Thr 
        515                 520                 525             


Ser Phe Gln Asp Ile Thr Glu Leu Lys Leu Ser Cys Met Ala Asp Tyr 
    530                 535                 540                 


Gly Ala Pro Val Phe Pro Phe Leu Ala Val Gly Gly Tyr Ser Gly Leu 
545                 550                 555                 560 


Glu Tyr Ser Glu Ala Gln Ala Leu Ile Leu Thr Phe Ser Leu Asn Asn 
                565                 570                 575     


Phe Leu Ser Ser Asp Pro Tyr Phe Asp Phe Val Met Leu Trp Glu Lys 
            580                 585                 590         


His Phe Leu Lys Val Val Gln Glu Phe Gln Lys Asn His Thr Glu Lys 
        595                 600                 605             


Tyr Thr Val Ala Tyr Met Ala Glu Arg Ser Leu Glu Asp Glu Val Ser 
    610                 615                 620                 


Arg Thr Thr Trp Glu Asp Leu Pro Ile Phe Leu Thr Ser Tyr Leu Val 
625                 630                 635                 640 


Ile Phe Val Tyr Met Ala Val Ala Leu Gly Glu Tyr Ser Ser Cys Arg 
                645                 650                 655     


Arg Ile Leu Val Asp Ala Lys Val Thr Ile Ala Leu Gly Gly Val Leu 
            660                 665                 670         


Val Val Leu Gly Ala Val Leu Ser Ser Leu Gly Phe Phe Ser Phe Val 
        675                 680                 685             


Gly Leu Pro Ser Ser Leu Ile Ile Leu Glu Val Val Pro Phe Leu Val 
    690                 695                 700                 


Leu Ala Val Gly Ala Asp Asn Ile Phe Ile Phe Val Leu Glu Tyr Gln 
705                 710                 715                 720 


Arg Ser Gln Pro Leu Pro Gly Glu Thr Pro Glu Gln His Leu Gly Arg 
                725                 730                 735     


Thr Leu Gly Asp Val Ala Pro Gly Met Leu Leu Cys Ser Leu Ser Glu 
            740                 745                 750         


Val Ile Cys Phe Phe Leu Gly Ala Leu Thr Pro Met Pro Ala Ile Trp 
        755                 760                 765             


Thr Phe Ala Leu Ser Ala Ala Leu Ala Val Leu Phe Asp Phe Leu Leu 
    770                 775                 780                 


Gln Ile Ser Leu Phe Val Ala Leu Met Ser Leu Asp Val Arg Arg Gln 
785                 790                 795                 800 


Lys Ala Ser Arg Ala Asp Leu Cys Cys Cys Leu Arg Leu Ala Glu His 
                805                 810                 815     


Gly Pro Val Lys Pro His Glu Gly Leu Leu Arg Thr Phe Met Arg Arg 
            820                 825                 830         


Phe Tyr Thr Pro Ala Leu Leu Gly Ser Ser Ile Val Arg Ala Leu Val 
        835                 840                 845             


Val Leu Phe Phe Thr Leu Leu Phe Cys Ala Gly Ile Phe Leu Thr Phe 
    850                 855                 860                 


His Val Asp Val Gly Leu Glu Gln Glu Leu Ala Val Pro Gln Asp Ser 
865                 870                 875                 880 


Tyr Met Arg Leu Tyr Phe Gln Tyr Ile Asn Gln Tyr Phe Met Val Gly 
                885                 890                 895     


Ala Pro Thr Tyr Phe Val Ala Thr Ala Gly Tyr Asn Phe Ser Thr Val 
            900                 905                 910         


Pro Gly Met Asn Ala Ile Cys Ser Ser Val Gly Cys Asn Asn Asn Ser 
        915                 920                 925             


Met Thr Gln Lys Ile Gln Thr Ala Thr Arg Phe Pro Glu Lys Ser Phe 
    930                 935                 940                 


Leu Ala Ile Pro Ala Ser Ser Trp Val Asp Asp Phe Ile Asp Trp Leu 
945                 950                 955                 960 


Asn Pro Leu Ser Arg Cys Cys Arg Ile His Ala Tyr Gly Pro Glu Lys 
                965                 970                 975     


Gly Glu Phe Cys Pro Ser Thr Asn Arg Lys Xaa Phe Cys Val Arg Arg 
            980                 985                 990         


Cys Ile Thr Thr Phe Ser Gly Thr  Leu Arg Pro Asn Thr  Glu Glu Phe 
        995                 1000                 1005             


Asn Lys  Phe Leu Pro Trp Phe  Leu His Asp Leu Pro  Asn Leu Gln 
    1010                 1015                 1020             


Cys Pro  Lys Gly Gly Leu Gly  Ala Tyr Asp Thr Ser  Val Arg Leu 
    1025                 1030                 1035             


Gly Pro  Asp Gly Gln Ile Leu  Ala Ser Arg Phe Met  Ala Tyr His 
    1040                 1045                 1050             


Thr Pro  Leu Lys Thr Ser Gln  Gln Tyr Thr Ala Ala  Leu Lys Ala 
    1055                 1060                 1065             


Val Arg  Glu Leu Ala Ala Asp  Ile Thr Ala Ile Leu  Arg Glu Val 
    1070                 1075                 1080             


Pro Gly  Thr Asp Pro Asn Phe  Gln Val Phe Pro Tyr  Thr Ile Thr 
    1085                 1090                 1095             


Tyr Val  Phe Phe Glu Gln Tyr  Leu Thr Leu Thr Ile  Val Gly Ile 
    1100                 1105                 1110             


Ile Asn  Leu Ala Leu Cys Leu  Val Pro Thr Phe Ala  Val Cys Tyr 
    1115                 1120                 1125             


Ile Leu  Leu Gly Met Asp Leu  Arg Ser Ala Phe Leu  Asn Leu Leu 
    1130                 1135                 1140             


Thr Ile  Ile Met Ile Val Val  Asp Thr Val Gly Ala  Met Thr Leu 
    1145                 1150                 1155             


Trp Gly  Val Ser Tyr Asn Ala  Ile Ser Leu Ile Asn  Leu Val Thr 
    1160                 1165                 1170             


Ala Val  Gly Ile Ser Val Glu  Phe Ile Ser His Val  Thr His Ser 
    1175                 1180                 1185             


Phe Ala  Val Ser Thr Arg Pro  Thr Lys Leu Glu Arg  Ser Arg Glu 
    1190                 1195                 1200             


Ala Thr  Ile Asn Met Gly Ser  Ala Val Phe Ala Gly  Val Ala Met 
    1205                 1210                 1215             


Thr Asn  Leu Pro Gly Ile Val  Val Leu Ala Phe Ala  Lys Ala Gln 
    1220                 1225                 1230             


Leu Ile  Gln Leu Phe Phe Phe  Arg Leu Asn Leu Ile  Ile Thr Leu 
    1235                 1240                 1245             


Leu Gly  Thr Leu His Gly Leu  Val Phe Leu Pro Val  Ile Leu Ser 
    1250                 1255                 1260             


Tyr Phe  Gly Pro Ala Val Arg  Gln Thr Thr Ala Gln  Val Gln Pro 
    1265                 1270                 1275             


Arg Thr  Glu Asn Ser Pro Ile  Pro Leu Glu Asp Lys  Glu Val His 
    1280                 1285                 1290             


Ser Ala  Asp Gln Thr Ser Leu  Ser His Arg Gln Ala  Pro Ala Pro 
    1295                 1300                 1305             


Ser Cys  Asp Leu Asp Arg Ala  Gly Thr 
    1310                 1315         


<210>  25
<211>  1049
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NPC1L1

<400>  25

Met Pro Gly Ser Ala Ala Leu Ala Gly Ala Val Leu Ser Ala Phe Leu 
1               5                   10                  15      


Ala Leu Ala Ala Pro Thr Phe Thr Pro Ile His Arg Ala Gly Tyr Cys 
            20                  25                  30          


Ala Phe Tyr Gly Glu Cys Gly Arg Asn Pro Glu Val Asn Ile Ser Leu 
        35                  40                  45              


Val Ser Ser Lys Val Pro Cys Leu Ser Asn Thr Pro Ala Arg Val Ala 
    50                  55                  60                  


Thr Gly Pro Leu Leu Ala Leu Leu Arg Thr Val Cys Pro Glu Leu Ala 
65                  70                  75                  80  


Arg Gly Ser Ala Glu Thr Thr Arg Val Cys Cys Ser Leu Ala Gln Leu 
                85                  90                  95      


Ser Ala Leu Gln Leu Ser Val Ala Leu Ser Gly Thr Val Leu Ser Arg 
            100                 105                 110         


Cys Pro Ala Cys Ala Arg Asn Phe Ala Asn Leu Tyr Cys Asn Asn Ile 
        115                 120                 125             


Cys Ser Pro Asp Gln Ser Leu Phe Thr Asn Val Thr Arg Val Ile Asn 
    130                 135                 140                 


Arg Thr Thr Ala Gln Gly Thr Pro Gln Leu Ala Val Val Glu Tyr Gln 
145                 150                 155                 160 


Cys Phe Tyr Arg Gln Tyr Phe Ala Asp Asp Ser Phe Ala Ser Cys Arg 
                165                 170                 175     


Gly Val Arg Leu Pro Ala Thr Gly Gly Tyr Ala Ile Asp Thr Met Cys 
            180                 185                 190         


Gly Arg Tyr Gly Ala Arg Leu Cys Thr Thr Gln Arg Trp Leu Asp Phe 
        195                 200                 205             


Gln Gly Asp Lys Asn Asn Gly Leu Ala Pro Leu Gln Ile Asp Phe Gln 
    210                 215                 220                 


Leu Val Pro Asn Gly Thr Gln Pro Gly Asp Ala Val Val Pro Leu Gly 
225                 230                 235                 240 


Gly Thr Ala Trp Arg Cys Asn Gln Ala Leu Ser Ala Gln Glu Gln Pro 
                245                 250                 255     


Cys Ser Cys Gln Asp Cys Ala Glu Ser Cys Pro Pro Val Ile Ala Pro 
            260                 265                 270         


Pro Ala Pro Gln Pro His Phe Leu Leu Gly Asn Ala Asn Gly Ala Leu 
        275                 280                 285             


Val Leu Cys Gly Leu Leu Phe Gly His Leu Ala Leu Val Phe Val Val 
    290                 295                 300                 


Ser Leu Leu Cys Gln Cys Trp Arg Ala Lys Lys Lys Lys Gly Thr Ala 
305                 310                 315                 320 


Pro Gln Pro Ala Pro Ala Pro Ala Ala Gly Cys Ser Ala Arg Leu Gly 
                325                 330                 335     


Asp Ala Ser His Arg Val Leu Ala Arg Ala Phe Arg Arg Trp Gly Thr 
            340                 345                 350         


Leu Val Ala Gly His Pro Val Thr Val Leu Ala Val Val Ala Val Leu 
        355                 360                 365             


Ala Gly Gly Leu Ser Ala Gly Leu Val Thr Leu Arg Leu Thr Thr Asp 
    370                 375                 380                 


Pro Val Glu Leu Trp Ser Ala Pro Gly Ser Arg Ala Arg Gln Glu Lys 
385                 390                 395                 400 


Ala Phe Tyr Asp Arg His Phe Gly Pro Phe Phe Arg Thr Asn Gln Val 
                405                 410                 415     


Ile Val Thr Ala Pro Gly Arg Pro Ser Ser Gly Tyr Glu Ser Val Val 
            420                 425                 430         


Leu Gly Ala Lys Asn Phe Ser Gly Val Leu Ser Glu Glu Val Leu Arg 
        435                 440                 445             


Ala Leu Leu Glu Leu Gln Glu Arg Leu Ala Ala Thr Thr Ala Trp Ala 
    450                 455                 460                 


Pro Val Ala Gly Arg Glu Val Thr Leu Ser Asp Val Cys Tyr Ala Pro 
465                 470                 475                 480 


Leu Asn Pro Thr Gln Pro Gly Leu Ala Asp Cys Cys Val Asn Ser Val 
                485                 490                 495     


Thr Gln Tyr Phe Gln Asn Asn Gly Thr Arg Leu Ala Met Thr Ala Thr 
            500                 505                 510         


Gln Thr Gly Lys Lys Thr Gly Thr Val Asp Trp His Asp His Leu Ile 
        515                 520                 525             


Tyr Cys Val Asn Ser Pro Leu Ser Phe Lys Asp Ile Thr Ala Leu Glu 
    530                 535                 540                 


Leu Ser Cys Met Ala Glu Tyr Gly Gly Pro Val Phe Pro Tyr Ile Ala 
545                 550                 555                 560 


Phe Gly Gly Tyr Pro Gly Ser Glu Tyr Thr Glu Ala Glu Ala Leu Ile 
                565                 570                 575     


Val Thr Tyr Ser Leu Asn Asn Phe Pro Arg Asp Asp Pro Arg His Glu 
            580                 585                 590         


Trp Val Leu Ser Trp Glu Arg Arg Phe Leu Glu Val Val Gly Asp Phe 
        595                 600                 605             


Gln Arg Ala His Gly Pro Asn Leu Ser Val Ala Phe Met Ala Glu Arg 
    610                 615                 620                 


Ser Leu Glu Asp Glu Ile Asn Arg Thr Thr Gly Glu Asp Ile Pro Val 
625                 630                 635                 640 


Phe Ala Ile Ser Tyr Leu Val Val Phe Ala Tyr Ile Ala Leu Ala Leu 
                645                 650                 655     


Gly Glu Tyr Thr Ala Trp Arg Arg Val Leu Val Glu Ser Lys Val Thr 
            660                 665                 670         


Leu Ala Leu Gly Gly Ile Ala Val Val Leu Gly Ala Val Phe Ala Ser 
        675                 680                 685             


Met Gly Phe Leu Ala Leu Leu Gly Leu Pro Ser Ser Leu Val Ile Leu 
    690                 695                 700                 


Glu Val Val Pro Phe Leu Val Leu Ala Val Gly Ala Asp Asn Ile Phe 
705                 710                 715                 720 


Ile Phe Val Gln Glu Tyr Gln Gln Ser Gln Arg Glu Pro Gly Glu Thr 
                725                 730                 735     


Arg Glu Gln His Val Gly Gln Val Leu Ala Arg Val Ala Pro Ser Met 
            740                 745                 750         


Leu Leu Cys Ser Leu Ser Glu Val Ile Cys Phe Phe Leu Gly Ala Leu 
        755                 760                 765             


Ser Ser Met Pro Ala Val Arg Thr Phe Ala Leu Thr Ala Ala Val Ala 
    770                 775                 780                 


Ile Ala Phe Asp Phe Leu Leu Gln Met Ser Gly Phe Val Ala Leu Leu 
785                 790                 795                 800 


Ala Leu Asp Ala Arg Arg Gln Glu Ala Ala Arg Phe Asp Leu Cys Cys 
                805                 810                 815     


Cys Cys Gly Met Gly Lys Gly Gly Pro Ala Gly Pro Gly Ala Arg Leu 
            820                 825                 830         


Leu Arg Pro Leu Leu His Arg Tyr Tyr Thr Pro Ser Val Leu Leu Phe 
        835                 840                 845             


Leu Phe Leu Ala Cys Ala Gly Leu Tyr Phe Thr Leu Gln Val Pro Val 
    850                 855                 860                 


Gly Leu Asp Gln Glu Leu Ala Met Pro Lys Asp Ser Tyr Met Leu Glu 
865                 870                 875                 880 


Tyr Phe Ala Ala Leu Asn Gln Tyr Leu Ala Val Gly Val Pro Thr Tyr 
                885                 890                 895     


Phe Val Thr Thr Gly Gly Tyr Asn Phe Ser Ser Pro Ala Gly Ile Asn 
            900                 905                 910         


Ser Ile Cys Ser Ser Ala Gly Cys Ala Thr Asp Ser Leu Thr His Thr 
        915                 920                 925             


Ile Gln Tyr Ala Thr Arg Phe Pro Asn Val Ser Tyr Leu Ala Ile Pro 
    930                 935                 940                 


Ala Thr Ser Trp Val Asp Asp Phe Leu Asp Trp Leu Asn Pro Thr Ser 
945                 950                 955                 960 


Arg Cys Cys Arg Ile His Arg Phe Gly Asn Leu Thr Gly Glu Phe Cys 
                965                 970                 975     


Pro Ser Thr Ser Ser Asp Pro Ser Cys Leu Gly Gly Gln Cys Leu Asn 
            980                 985                 990         


Ala Thr Arg Arg Pro Thr Val Glu  Glu Phe Glu Arg Phe  Leu Pro Trp 
        995                 1000                 1005             


Phe Leu  His Asp His Pro Thr  Leu Gln Cys Ala Lys  Gly Trp Ser 
    1010                 1015                 1020             


Glu Asp  Leu Gly Cys Trp Ala  Gln Pro Gln Met Arg  Glu Pro Gln 
    1025                 1030                 1035             


Gly Phe  Leu His Arg Gly Gly  Leu Cys Gly Arg 
    1040                 1045                 


