                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
       Janssen Biotech, Inc.
 
<120>  Novel AAV-Mediated Influenza Vaccines

<130>  17-7987PCT

<150>  62/464753
<151>  2017-02-28

<150>  62/504293
<151>  2017-05-10

<150>  62/560834
<151>  2017-09-20

<150>  62/618443
<151>  2017-01-17

<160>  39    

<170>  PatentIn version 3.5

<210>  1
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the first immunoglobulin region

<400>  1

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Ile Ser Ile Phe Asp Ile Tyr 
            20                  25                  30          


Ala Met Asp Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Val Ser Phe Arg Asp Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys His 
                85                  90                  95      


Val Ser Leu Tyr Arg Asp Pro Leu Gly Val Ala Gly Gly Ile Gly Val 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 


<210>  2
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the second immunoglobulin region

<400>  2

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Tyr Ala Met Gly 
            20                  25                  30          


Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val Ala Ala Ile 
        35                  40                  45              


Asn Ala Leu Gly Thr Arg Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg 
    50                  55                  60                  


Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met 
65                  70                  75                  80  


Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Thr Ala Gln 
                85                  90                  95      


Gly Gln Trp Arg Ala Ala Pro Val Ala Val Ala Ala Glu Tyr Glu Phe 
            100                 105                 110         


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120             


<210>  3
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the third immunoglobulin region

<400>  3

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Leu Glu Asn Lys 
            20                  25                  30          


Ala Ile Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Leu Cys Ile Ser Lys Ser Gly Ser Trp Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Thr Thr Ala Gly Gly Gly Leu Cys Trp Asp Gly Thr Thr Phe 
            100                 105                 110         


Ser Arg Leu Ala Ser Ser Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
        115                 120                 125             


Ser 
    


<210>  4
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the fourth immunoglobulin region

<400>  4

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Ser 
            20                  25                  30          


Trp Met Tyr Trp Leu Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Val Ile Asn Thr Asp Gly Gly Thr Tyr Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Lys Asp Trp Gly Gly Pro Glu Pro Thr Arg Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  5
<211>  4718
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  J210a Vector Genome with CB7 promoter and rBG polyA


<220>
<221>  repeat_region
<222>  (1)..(130)
<223>  5' ITR

<220>
<221>  promoter
<222>  (198)..(579)
<223>  CMV IE promoter

<220>
<221>  enhancer
<222>  (279)..(538)
<223>  C4 enhancer

<220>
<221>  promoter
<222>  (582)..(862)
<223>  chicken b-actin (CB) promoter

<220>
<221>  TATA_signal
<222>  (836)..(839)

<220>
<221>  Intron
<222>  (956)..(1928)
<223>  chicken beta-actin intron

<220>
<221>  5'UTR
<222>  (1938)..(1985)
<223>  c-myc 5'UTR

<220>
<221>  misc_feature
<222>  (1986)..(1991)
<223>  kozak

<220>
<221>  misc_feature
<222>  (1992)..(2051)
<223>  hmIL2 leader

<220>
<221>  misc_feature
<222>  (2052)..(2423)
<223>  The first immunoglobulin region

<220>
<221>  misc_feature
<222>  (2424)..(2453)
<223>  L1

<220>
<221>  misc_feature
<222>  (2454)..(2822)
<223>  The second immunoglobulin region

<220>
<221>  misc_feature
<222>  (2823)..(2852)
<223>  L2

<220>
<221>  misc_feature
<222>  (2853)..(3239)
<223>  The third immunoglobulin region

<220>
<221>  misc_feature
<222>  (3240)..(3269)
<223>  L3

<220>
<221>  misc_feature
<222>  (3270)..(3617)
<223>  The fourth immunoglobulin region

<220>
<221>  misc_feature
<222>  (3618)..(3632)
<223>  hinge

<220>
<221>  misc_feature
<222>  (3633)..(4298)
<223>  Fc

<220>
<221>  polyA_signal
<222>  (4374)..(4500)
<223>  rabbit globin polyA

<220>
<221>  repeat_region
<222>  (4589)..(4718)
<223>  3' ITR

<400>  5
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg      180

atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat      240

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      300

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      360

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      420

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      480

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc      540

tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac      600

gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat      660

tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg      720

ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca      780

gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa      840

aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc      900

cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag      960

cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt     1020

ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg     1080

gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc     1140

gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt     1200

gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa     1260

caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt     1320

cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg     1380

tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca     1440

ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg     1500

cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt     1560

atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa     1620

atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg     1680

caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc     1740

tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc     1800

ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc     1860

cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt     1920

tggcaaagaa ttcgctaggg cactttgcac tggaacttac aacacccgag caaggacgcg     1980

actctgccac catgtaccga atgcagctgc tgagctgtat cgcactgagc ctggcactgg     2040

tgaccaacag ccaggtgcag ctggtggaga gcggaggagg agtggtgcag ccaggaggaa     2100

gcctgcgact gagctgtgca gcaagcatca gcatcttcga catctacgca atggactggt     2160

accgacaggc accaggaaag cagcgagagc tggtggcagt gagcttccga gacggaagca     2220

cctactacgc agacagcgtg aagggacgat tcaccatcag ccgagacaac agcaagaaca     2280

ccctgtacct gcagatgaac agcctgcgag cagaggacac cgcagtgtac tactgtcatg     2340

tgagcctgta ccgggaccca ctgggagtgg caggaggaat cggagtgtac tggggacagg     2400

gaaccctggt gaccgtgagc agcggaggag gaggaagcgg aggaggagga agcgaagtgc     2460

agctgctgga aagcggcggc ggcctggtgc agcccggcgg cagcctgcgg ctgagctgtg     2520

ctgctagcgg ccggacctac gctatgggct ggtttcggca ggctcccggc aaggaacggg     2580

aatttgtggc tgctattaac gctctgggca cccggaccta ttatgctgac agcgtgaagg     2640

gccggtttac cattagccgg gacaacagca agaacaccct gtatctgcag atgaacagcc     2700

tgcgggctga agacaccgct gtgtattatt gtaccgctca gggccagtgg cgggctgctc     2760

ccgtggctgt ggctgctgaa tacgaatttt ggggccaggg caccctggtg accgtgagca     2820

gcggaggagg aggatctgga ggaggaggat ctgaggtgca gctgctggag agcggaggag     2880

gactggtgca gccaggagga agcctgcggc tgagctgcgc cgccagcgga ttcaccctgg     2940

agaacaaggc catcggctgg ttccggcagg ccccaggaaa ggagcgggag ggagtgctgt     3000

gcatcagcaa gagcggaagc tggacctact acgccgatag cgtgaaggga cggttcacca     3060

tcagccggga taacagcaag aacaccgtgt acctgcagat gaacagcctg cggccagagg     3120

ataccgccgt gtactactgc gccaccacca ccgccggagg aggactgtgc tgggacggaa     3180

ccaccttcag ccggctggcc agcagctggg gacagggaac cctggtgacc gtgagcagcg     3240

gaggaggagg atccggagga ggaggatccg aggtgcagct ggtggagtca ggaggaggac     3300

tggtgcagcc cggaggatca ctgagactgt cctgcgctgc ttcaggattc accttctcaa     3360

cctcctggat gtattggctg agacaggctc ccggaaaagg actggagtgg gtgtcagtga     3420

tcaacaccga cggaggaacc tattatgctg attcagtgaa aggaagattc accatctcaa     3480

gagataactc aaaaaacacc ctgtatctgc agatgaactc actgagagct gaggataccg     3540

ctgtgtatta ttgcgctaaa gattggggag gacccgagcc caccagagga cagggaaccc     3600

tggtgaccgt gtcatcagac aagacccata cctgtccacc ttgtccagca ccagagctgc     3660

tgggaggacc aagcgtgttc ctgttcccac caaagccaaa ggacaccctg atgatcagcc     3720

gaaccccaga ggtgacctgt gtggtggtgg acgtgagcca tgaggaccca gaggtgaagt     3780

tcaactggta cgtggacgga gtggaggtgc ataacgcaaa gaccaagcca cgagaggagc     3840

agtacaacag cacctaccga gtggtgagcg tgctgaccgt gctgcatcag gactggctga     3900

acggaaagga gtacaagtgt aaggtgagca acaaggcact gccagcacca atcgagaaga     3960

ccatcagcaa ggcaaaggga cagccacgag agccacaggt gtacaccctg ccaccaagcc     4020

gagacgagct gaccaagaac caggtgagcc tgacctgtct ggtgaaggga ttctacccaa     4080

gcgacatcgc agtggagtgg gagagcaacg gacagccaga gaacaactac aagaccaccc     4140

caccagtgct ggacagcgac ggaagcttct tcctgtacag caagctgacc gtggacaaga     4200

gccggtggca gcagggaaac gtgttcagct gtagcgtgat gcacgaggca ctgcataacc     4260

attacaccca gaagagcctg agcctgagcc caggaaagtg ataaagcggc cgcggtacct     4320

ctagagtcga cccgggcggc ctcgaggacg gggtgaacta cgcctgagga tccgatcttt     4380

ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct     4440

aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg     4500

gaagcaattc gttgatctga atttcgacca cccataatac ccattaccct ggtagataag     4560

tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc     4620

tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc     4680

tttgcccggg cggcctcagt gagcgagcga gcgcgcag                             4718


<210>  6
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of human IL2 (hmIL2) leader

<400>  6

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser 
            20  


<210>  7
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of linker 1 (L1)

<400>  7

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  8
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hinge


<220>
<221>  CDS
<222>  (1)..(15)
<223>  hinge

<400>  8
gac aag acc cat acc                                                     15
Asp Lys Thr His Thr                                                       
1               5                                                         


<210>  9
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  9

Asp Lys Thr His Thr 
1               5   


<210>  10
<211>  666
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fc


<220>
<221>  CDS
<222>  (1)..(666)

<400>  10
tgt cca cct tgt cca gca cca gag ctg ctg gga gga cca agc gtg ttc         48
Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe           
1               5                   10                  15                

ctg ttc cca cca aag cca aag gac acc ctg atg atc agc cga acc cca         96
Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro           
            20                  25                  30                    

gag gtg acc tgt gtg gtg gtg gac gtg agc cat gag gac cca gag gtg        144
Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val           
        35                  40                  45                        

aag ttc aac tgg tac gtg gac gga gtg gag gtg cat aac gca aag acc        192
Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr           
    50                  55                  60                            

aag cca cga gag gag cag tac aac agc acc tac cga gtg gtg agc gtg        240
Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val           
65                  70                  75                  80            

ctg acc gtg ctg cat cag gac tgg ctg aac gga aag gag tac aag tgt        288
Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys           
                85                  90                  95                

aag gtg agc aac aag gca ctg cca gca cca atc gag aag acc atc agc        336
Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser           
            100                 105                 110                   

aag gca aag gga cag cca cga gag cca cag gtg tac acc ctg cca cca        384
Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro           
        115                 120                 125                       

agc cga gac gag ctg acc aag aac cag gtg agc ctg acc tgt ctg gtg        432
Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val           
    130                 135                 140                           

aag gga ttc tac cca agc gac atc gca gtg gag tgg gag agc aac gga        480
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly           
145                 150                 155                 160           

cag cca gag aac aac tac aag acc acc cca cca gtg ctg gac agc gac        528
Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp           
                165                 170                 175               

gga agc ttc ttc ctg tac agc aag ctg acc gtg gac aag agc cgg tgg        576
Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp           
            180                 185                 190                   

cag cag gga aac gtg ttc agc tgt agc gtg atg cac gag gca ctg cat        624
Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His           
        195                 200                 205                       

aac cat tac acc cag aag agc ctg agc ctg agc cca gga aag                666
Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys                   
    210                 215                 220                           


<210>  11
<211>  222
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  11

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
1               5                   10                  15      


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
            20                  25                  30          


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
        35                  40                  45              


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
    50                  55                  60                  


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
65                  70                  75                  80  


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
                85                  90                  95      


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
            100                 105                 110         


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
        115                 120                 125             


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
    130                 135                 140                 


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
145                 150                 155                 160 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
                165                 170                 175     


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
            180                 185                 190         


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
        195                 200                 205             


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215                 220         


<210>  12
<211>  746
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized FM1W3

<400>  12

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Trp Gln Pro Gly Gly Ser 
1               5                   10                  15      


Leu Arg Leu Ser Cys Ala Ala Ser Ile Ser Ile Phe Asp Ile Tyr Ala 
            20                  25                  30          


Met Asp Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val Ala 
        35                  40                  45              


Val Ser Phe Arg Asp Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln 
65                  70                  75                  80  


Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys His Val 
                85                  90                  95      


Ser Leu Tyr Arg Asp Pro Leu Gly Val Ala Gly Gly Ile Gly Val Tyr 
            100                 105                 110         


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu 
    130                 135                 140                 


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg 
145                 150                 155                 160 


Thr Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
                165                 170                 175     


Phe Val Ala Ala Ile Asn Ala Leu Gly Thr Arg Thr Tyr Tyr Ala Asp 
            180                 185                 190         


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr 
        195                 200                 205             


Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
    210                 215                 220                 


Tyr Cys Thr Ala Gln Gly Gln Trp Arg Ala Ala Pro Val Ala Val Ala 
225                 230                 235                 240 


Ala Glu Tyr Glu Phe Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
                245                 250                 255     


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Leu Glu 
            260                 265                 270         


Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys 
        275                 280                 285             


Ala Ala Ser Gly Phe Thr Leu Glu Asn Lys Ala Ile Gly Trp Phe Arg 
    290                 295                 300                 


Gln Ala Pro Gly Lys Glu Arg Glu Gly Val Leu Cys Ile Ser Lys Ser 
305                 310                 315                 320 


Gly Ser Trp Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile 
                325                 330                 335     


Ser Arg Asp Asn Ser Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu 
            340                 345                 350         


Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala Thr Thr Thr Ala Gly 
        355                 360                 365             


Gly Gly Leu Cys Trp Asp Gly Thr Thr Phe Ser Arg Leu Ala Ser Ser 
    370                 375                 380                 


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
385                 390                 395                 400 


Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
                405                 410                 415     


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
            420                 425                 430         


Thr Phe Ser Thr Ser Trp Met Tyr Trp Leu Arg Gln Ala Pro Gly Lys 
        435                 440                 445             


Gly Leu Glu Trp Val Ser Val Ile Asn Thr Asp Gly Gly Thr Tyr Tyr 
    450                 455                 460                 


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
465                 470                 475                 480 


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
                485                 490                 495     


Val Tyr Tyr Cys Ala Lys Asp Trp Gly Gly Pro Glu Pro Thr Arg Gly 
            500                 505                 510         


Gln Gly Thr Leu Val Thr Val Ser Ser Asp Lys Thr His Thr Cys Pro 
        515                 520                 525             


Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
    530                 535                 540                 


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
545                 550                 555                 560 


Thr Cys Val Trp Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn 
                565                 570                 575     


Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 
            580                 585                 590         


Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Trp Ser Val Leu Thr Val Leu 
        595                 600                 605             


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
    610                 615                 620                 


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
625                 630                 635                 640 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
                645                 650                 655     


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
            660                 665                 670         


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
        675                 680                 685             


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
    690                 695                 700                 


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
705                 710                 715                 720 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
                725                 730                 735     


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            740                 745     


<210>  13
<211>  4544
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  humanized sdAb multimer J310a


<220>
<221>  repeat_region
<222>  (17)..(146)
<223>  5' ITR

<220>
<221>  promoter
<222>  (207)..(1433)
<223>  UbC

<220>
<221>  Intron
<222>  (1527)..(1659)
<223>  Promega chimeric intron

<220>
<221>  5'UTR
<222>  (1726)..(1773)
<223>  c-myc 5'UTR

<220>
<221>  misc_feature
<222>  (1774)..(1779)
<223>  kozak

<220>
<221>  misc_feature
<222>  (1780)..(1839)
<223>  hmIL2 leader

<220>
<221>  misc_feature
<222>  (1840)..(2211)
<223>  The first immunoglobulin region

<220>
<221>  misc_binding
<222>  (2212)..(2241)
<223>  L1

<220>
<221>  misc_feature
<222>  (2242)..(2610)
<223>  The second immunoglobulin region

<220>
<221>  misc_binding
<222>  (2611)..(2640)
<223>  L2

<220>
<221>  misc_feature
<222>  (2641)..(3027)
<223>  The third immunoglobulin region

<220>
<221>  misc_binding
<222>  (3028)..(3057)
<223>  L3

<220>
<221>  misc_feature
<222>  (3058)..(3405)
<223>  The fourth immunoglobulin region

<220>
<221>  misc_binding
<222>  (3406)..(3435)
<223>  L4

<220>
<221>  misc_feature
<222>  (3436)..(4101)
<223>  Fc

<220>
<221>  polyA_signal
<222>  (4119)..(4350)
<223>  SV40 late polyA signal

<220>
<221>  repeat_region
<222>  (4415)..(4544)
<223>  3' ITR

<400>  13
taaggcctta attaggctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg       60

gcgtcgggcg acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt      120

ggccaactcc atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc      180

tacgtagcca tgctctagga agatctggcc tccgcgccgg gttttggcgc ctcccgcggg      240

cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagga gcgtcctgat      300

ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc      360

cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc      420

tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga      480

tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta      540

gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac      600

ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg      660

ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc      720

cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg      780

aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg      840

caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg      900

ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt      960

tgtcgtctgt tgcgggggcg gcagttatgc ggtgccgttg ggcagtgcac ccgtaccttt     1020

gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg     1080

tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg     1140

ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa     1200

gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc     1260

cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct     1320

tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc     1380

gctaaattct ggccgttttt ggcttttttg ttagacgaag ctttattgcg gtagtttatc     1440

acagttaaat tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca     1500

gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag     1560

accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta     1620

ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt     1680

acagctctta aggctagagt acttaatacg actcactata ggctagggca ctttgcactg     1740

gaacttacaa cacccgagca aggacgcgac tctgccacca tgtaccgaat gcagctgctg     1800

agctgtatcg cactgagcct ggcactggtg accaacagcc aggtgcagct ggtggagagc     1860

ggaggaggag tggtgcagcc aggaggaagc ctgcgactga gctgtgcagc aagcatcagc     1920

atcttcgaca tctacgcaat ggactggtac cgacaggcac caggaaagca gcgagagctg     1980

gtggcagtga gcttccgaga cggaagcacc tactacgcag acagcgtgaa gggacgattc     2040

accatcagcc gagacaacag caagaacacc ctgtacctgc agatgaacag cctgcgagca     2100

gaggacaccg cagtgtacta ctgtcatgtg agcctgtacc gggacccact gggagtggca     2160

ggaggaatcg gagtgtactg gggacaggga accctggtga ccgtgagcag cggaggagga     2220

ggaagcggag gaggaggaag cgaagtgcag ctgctggaaa gcggcggcgg cctggtgcag     2280

cccggcggca gcctgcggct gagctgtgct gctagcggcc ggacctacgc tatgggctgg     2340

tttcggcagg ctcccggcaa ggaacgggaa tttgtggctg ctattaacgc tctgggcacc     2400

cggacctatt atgctgacag cgtgaagggc cggtttacca ttagccggga caacagcaag     2460

aacaccctgt atctgcagat gaacagcctg cgggctgaag acaccgctgt gtattattgt     2520

accgctcagg gccagtggcg ggctgctccc gtggctgtgg ctgctgaata cgaattttgg     2580

ggccagggca ccctggtgac cgtgagcagc ggaggaggag gatctggagg aggaggatct     2640

gaggtgcagc tgctggagag cggaggagga ctggtgcagc caggaggaag cctgcggctg     2700

agctgcgccg ccagcggatt caccctggag aacaaggcca tcggctggtt ccggcaggcc     2760

ccaggaaagg agcgggaggg agtgctgtgc atcagcaaga gcggaagctg gacctactac     2820

gccgatagcg tgaagggacg gttcaccatc agccgggata acagcaagaa caccgtgtac     2880

ctgcagatga acagcctgcg gccagaggat accgccgtgt actactgcgc caccaccacc     2940

gccggaggag gactgtgctg ggacggaacc accttcagcc ggctggccag cagctgggga     3000

cagggaaccc tggtgaccgt gagcagcgga ggaggaggat ccggaggagg aggatccgag     3060

gtgcagctgg tggagtcagg aggaggactg gtgcagcccg gaggatcact gagactgtcc     3120

tgcgctgctt caggattcac cttctcaacc tcctggatgt attggctgag acaggctccc     3180

ggaaaaggac tggagtgggt gtcagtgatc aacaccgacg gaggaaccta ttatgctgat     3240

tcagtgaaag gaagattcac catctcaaga gataactcaa aaaacaccct gtatctgcag     3300

atgaactcac tgagagctga ggataccgct gtgtattatt gcgctaaaga ttggggagga     3360

cccgagccca ccagaggaca gggaaccctg gtgaccgtgt catcaggagg aggaggatca     3420

ggaggaggag gatcctgtcc accttgtcca gcaccagagc tgctgggagg accaagcgtg     3480

ttcctgttcc caccaaagcc aaaggacacc ctgatgatca gccgaacccc agaggtgacc     3540

tgtgtggtgg tggacgtgag ccatgaggac ccagaggtga agttcaactg gtacgtggac     3600

ggagtggagg tgcataacgc aaagaccaag ccacgagagg agcagtacaa cagcacctac     3660

cgagtggtga gcgtgctgac cgtgctgcat caggactggc tgaacggaaa ggagtacaag     3720

tgtaaggtga gcaacaaggc actgccagca ccaatcgaga agaccatcag caaggcaaag     3780

ggacagccac gagagccaca ggtgtacacc ctgccaccaa gccgagacga gctgaccaag     3840

aaccaggtga gcctgacctg tctggtgaag ggattctacc caagcgacat cgcagtggag     3900

tgggagagca acggacagcc agagaacaac tacaagacca ccccaccagt gctggacagc     3960

gacggaagct tcttcctgta cagcaagctg accgtggaca agagccggtg gcagcaggga     4020

aacgtgttca gctgtagcgt gatgcacgag gcactgcata accattacac ccagaagagc     4080

ctgagcctga gcccaggaaa gtgataaagc ggccgcttcg agcagacatg ataagataca     4140

ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa     4200

tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca     4260

acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca     4320

agtaaaacct ctacaaatgt ggtaaaatcg ataaggatct tcctagagca tggctacgta     4380

gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc     4440

actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc     4500

ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcag                      4544


<210>  14
<211>  4733
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CB7_J310a


<220>
<221>  repeat_region
<222>  (1)..(130)
<223>  5' ITR

<220>
<221>  promoter
<222>  (198)..(579)
<223>  CMV IE promoter

<220>
<221>  promoter
<222>  (582)..(862)
<223>  CB promoter

<220>
<221>  TATA_signal
<222>  (836)..(839)
<223>  TATA

<220>
<221>  Intron
<222>  (956)..(1928)
<223>  chicken beta-actin intron

<220>
<221>  5'UTR
<222>  (1938)..(1985)
<223>  c-myc 5'UTR

<220>
<221>  misc_feature
<222>  (1986)..(1991)
<223>  kozak

<220>
<221>  misc_feature
<222>  (1992)..(2051)
<223>  hmIL2 leader

<220>
<221>  misc_feature
<222>  (2052)..(2423)
<223>  The first immunoglobulin region

<220>
<221>  misc_binding
<222>  (2424)..(2453)
<223>  L1

<220>
<221>  misc_feature
<222>  (2454)..(2822)
<223>  The second immunoglobulin region

<220>
<221>  misc_binding
<222>  (2823)..(2852)
<223>  L2

<220>
<221>  misc_feature
<222>  (2853)..(3239)
<223>  The third immunoglobulin region

<220>
<221>  misc_binding
<222>  (3240)..(3269)
<223>  L3

<220>
<221>  misc_feature
<222>  (3270)..(3617)
<223>  The fourth immunoglobulin region

<220>
<221>  misc_binding
<222>  (3618)..(3647)
<223>  L4

<220>
<221>  misc_feature
<222>  (3648)..(4313)
<223>  Fc

<220>
<221>  polyA_signal
<222>  (4389)..(4515)
<223>  rabbit globin polyA

<220>
<221>  repeat_region
<222>  (4604)..(4733)
<223>  3' ITR

<400>  14
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg      180

atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat      240

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      300

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      360

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      420

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      480

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc      540

tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac      600

gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat      660

tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg      720

ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca      780

gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa      840

aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc      900

cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag      960

cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt     1020

ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg     1080

gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc     1140

gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt     1200

gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa     1260

caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt     1320

cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg     1380

tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca     1440

ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg     1500

cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt     1560

atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa     1620

atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg     1680

caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc     1740

tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc     1800

ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc     1860

cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt     1920

tggcaaagaa ttcgctaggg cactttgcac tggaacttac aacacccgag caaggacgcg     1980

actctgccac catgtaccga atgcagctgc tgagctgtat cgcactgagc ctggcactgg     2040

tgaccaacag ccaggtgcag ctggtggaga gcggaggagg agtggtgcag ccaggaggaa     2100

gcctgcgact gagctgtgca gcaagcatca gcatcttcga catctacgca atggactggt     2160

accgacaggc accaggaaag cagcgagagc tggtggcagt gagcttccga gacggaagca     2220

cctactacgc agacagcgtg aagggacgat tcaccatcag ccgagacaac agcaagaaca     2280

ccctgtacct gcagatgaac agcctgcgag cagaggacac cgcagtgtac tactgtcatg     2340

tgagcctgta ccgggaccca ctgggagtgg caggaggaat cggagtgtac tggggacagg     2400

gaaccctggt gaccgtgagc agcggaggag gaggaagcgg aggaggagga agcgaagtgc     2460

agctgctgga aagcggcggc ggcctggtgc agcccggcgg cagcctgcgg ctgagctgtg     2520

ctgctagcgg ccggacctac gctatgggct ggtttcggca ggctcccggc aaggaacggg     2580

aatttgtggc tgctattaac gctctgggca cccggaccta ttatgctgac agcgtgaagg     2640

gccggtttac cattagccgg gacaacagca agaacaccct gtatctgcag atgaacagcc     2700

tgcgggctga agacaccgct gtgtattatt gtaccgctca gggccagtgg cgggctgctc     2760

ccgtggctgt ggctgctgaa tacgaatttt ggggccaggg caccctggtg accgtgagca     2820

gcggaggagg aggatctgga ggaggaggat ctgaggtgca gctgctggag agcggaggag     2880

gactggtgca gccaggagga agcctgcggc tgagctgcgc cgccagcgga ttcaccctgg     2940

agaacaaggc catcggctgg ttccggcagg ccccaggaaa ggagcgggag ggagtgctgt     3000

gcatcagcaa gagcggaagc tggacctact acgccgatag cgtgaaggga cggttcacca     3060

tcagccggga taacagcaag aacaccgtgt acctgcagat gaacagcctg cggccagagg     3120

ataccgccgt gtactactgc gccaccacca ccgccggagg aggactgtgc tgggacggaa     3180

ccaccttcag ccggctggcc agcagctggg gacagggaac cctggtgacc gtgagcagcg     3240

gaggaggagg atccggagga ggaggatccg aggtgcagct ggtggagtca ggaggaggac     3300

tggtgcagcc cggaggatca ctgagactgt cctgcgctgc ttcaggattc accttctcaa     3360

cctcctggat gtattggctg agacaggctc ccggaaaagg actggagtgg gtgtcagtga     3420

tcaacaccga cggaggaacc tattatgctg attcagtgaa aggaagattc accatctcaa     3480

gagataactc aaaaaacacc ctgtatctgc agatgaactc actgagagct gaggataccg     3540

ctgtgtatta ttgcgctaaa gattggggag gacccgagcc caccagagga cagggaaccc     3600

tggtgaccgt gtcatcagga ggaggaggat caggaggagg aggatcctgt ccaccttgtc     3660

cagcaccaga gctgctggga ggaccaagcg tgttcctgtt cccaccaaag ccaaaggaca     3720

ccctgatgat cagccgaacc ccagaggtga cctgtgtggt ggtggacgtg agccatgagg     3780

acccagaggt gaagttcaac tggtacgtgg acggagtgga ggtgcataac gcaaagacca     3840

agccacgaga ggagcagtac aacagcacct accgagtggt gagcgtgctg accgtgctgc     3900

atcaggactg gctgaacgga aaggagtaca agtgtaaggt gagcaacaag gcactgccag     3960

caccaatcga gaagaccatc agcaaggcaa agggacagcc acgagagcca caggtgtaca     4020

ccctgccacc aagccgagac gagctgacca agaaccaggt gagcctgacc tgtctggtga     4080

agggattcta cccaagcgac atcgcagtgg agtgggagag caacggacag ccagagaaca     4140

actacaagac caccccacca gtgctggaca gcgacggaag cttcttcctg tacagcaagc     4200

tgaccgtgga caagagccgg tggcagcagg gaaacgtgtt cagctgtagc gtgatgcacg     4260

aggcactgca taaccattac acccagaaga gcctgagcct gagcccagga aagtgataaa     4320

gcggccgcgg tacctctaga gtcgacccgg gcggcctcga ggacggggtg aactacgcct     4380

gaggatccga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc     4440

atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt     4500

tgtgtctctc actcggaagc aattcgttga tctgaatttc gaccacccat aatacccatt     4560

accctggtag ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg     4620

gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc     4680

gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cag            4733


<210>  15
<211>  4718
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CB7 J2201


<220>
<221>  repeat_region
<222>  (1)..(130)
<223>  5' ITR

<220>
<221>  promoter
<222>  (198)..(579)
<223>  CMV IE promoter

<220>
<221>  promoter
<222>  (582)..(862)
<223>  CB promoter

<220>
<221>  TATA_signal
<222>  (836)..(839)

<220>
<221>  Intron
<222>  (956)..(1928)
<223>  chicken beta-acin intron

<220>
<221>  5'UTR
<222>  (1938)..(1985)
<223>  c-myc 5'UTR

<220>
<221>  misc_feature
<222>  (1986)..(1991)
<223>  kozak

<220>
<221>  misc_feature
<222>  (1992)..(2051)
<223>  hmIL2 leader

<220>
<221>  misc_feature
<222>  (2052)..(2423)
<223>  The first immunoglobulin region

<220>
<221>  misc_binding
<222>  (2424)..(2453)
<223>  L1

<220>
<221>  misc_feature
<222>  (2454)..(2822)
<223>  The second immunoglobulin region

<220>
<221>  misc_binding
<222>  (2823)..(2852)
<223>  L2

<220>
<221>  misc_feature
<222>  (2853)..(3239)
<223>  The third immunoglobulin region

<220>
<221>  misc_binding
<222>  (3240)..(3269)
<223>  L3

<220>
<221>  misc_feature
<222>  (3270)..(3617)
<223>  The fourth immunoglobulin region

<220>
<221>  misc_binding
<222>  (3618)..(3632)
<223>  hinge

<220>
<221>  misc_feature
<222>  (3633)..(4298)
<223>  Fc

<220>
<221>  polyA_signal
<222>  (4374)..(4500)
<223>  Rabbit globin poly A

<220>
<221>  repeat_region
<222>  (4589)..(4718)
<223>  3' ITR

<400>  15
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg      180

atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat      240

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      300

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      360

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      420

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      480

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc      540

tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac      600

gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat      660

tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg      720

ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca      780

gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa      840

aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc      900

cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag      960

cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt     1020

ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg     1080

gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc     1140

gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt     1200

gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa     1260

caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt     1320

cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg     1380

tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca     1440

ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg     1500

cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt     1560

atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa     1620

atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg     1680

caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc     1740

tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc     1800

ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc     1860

cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt     1920

tggcaaagaa ttcgctaggg cactttgcac tggaacttac aacacccgag caaggacgcg     1980

actctgccac catgtaccgg atgcagctgc tgagctgcat cgccctgagc ctggccctgg     2040

tgaccaacag ccaggtgcag ctggtggaga gcggaggagg agtggtgcag ccaggaggaa     2100

gcctgcggct gagctgcgcc gccagcatca gcatcttcga tatctacgcc atggattggt     2160

accggcaggc cccaggaaag cagcgggagc tggtggccgt gagcttccgg gacggaagca     2220

cctactacgc cgatagcgtg aagggacggt tcaccatcag ccgggataac agcaagaaca     2280

ccctgtacct gcagatgaac agcctgcggg ccgaggatac cgccgtgtac tactgccacg     2340

tgagcctgta ccgggatcca ctgggagtgg ccggaggaat cggagtgtac tggggacagg     2400

gaaccctggt gaccgtgagc agcggaggag gaggaagcgg aggaggagga agcgaggtgc     2460

agctgctgga gagcggagga ggactggtgc agccaggagg aagcctgcga ctgagctgtg     2520

cagcaagcgg acgaacctac gcaatgggct ggttccgaca ggcaccagga aaggagcgag     2580

agttcgtggc agcaatcaac gcactgggaa cccgaaccta ctacgcagac agcgtgaagg     2640

gacgattcac catcagccga gacaacagca agaacaccct gtacctgcag atgaacagcc     2700

tgcgagcaga ggacaccgca gtgtactact gtaccgcaca gggacagtgg cgagcagcac     2760

cagtggcagt ggcagcagag tacgagttct ggggacaggg aaccctggtg accgtgagca     2820

gcggaggagg aggatctgga ggaggaggat ctgaagtgca gctgctggaa agcggcggcg     2880

gcctggtgca gcccggcggc agcctgcggc tgagctgtgc tgctagcggc tttaccctgg     2940

aaaacaaggc tattggctgg tttcggcagg ctcccggcaa ggaacgggaa ggcgtgctgt     3000

gtattagcaa gagcggcagc tggacctatt atgctgacag cgtgaagggc cggtttacca     3060

ttagccggga caacagcaag aacaccgtgt atctgcagat gaacagcctg cggcccgaag     3120

acaccgctgt gtattattgt gctaccacca ccgctggcgg cggcctgtgt tgggacggca     3180

ccacctttag ccggctggct agcagctggg gccagggcac cctggtgacc gtgagcagcg     3240

gaggaggagg atccggagga ggaggatccg aggtgcagct ggtggagtca ggaggaggac     3300

tggtgcagcc cggaggatca ctgagactgt cctgcgctgc ttcaggattc accttctcaa     3360

cctcctggat gtattggctg agacaggctc ccggaaaagg actggagtgg gtgtcagtga     3420

tcaacaccga cggaggaacc tattatgctg attcagtgaa aggaagattc accatctcaa     3480

gagataactc aaaaaacacc ctgtatctgc agatgaactc actgagagct gaggataccg     3540

ctgtgtatta ttgcgctaaa gattggggag gacccgagcc caccagagga cagggaaccc     3600

tggtgaccgt gtcatcagat aagacccaca cttgcccacc ttgcccagcc ccagagctgc     3660

tgggaggacc aagcgtgttc ctgttcccac caaagccaaa ggataccctg atgatcagcc     3720

ggaccccaga ggtgacctgc gtggtggtgg atgtgagcca cgaggatcca gaggtgaagt     3780

tcaactggta cgtggacgga gtggaggtgc acaacgccaa gaccaagcca cgggaggagc     3840

agtacaacag cacctaccgg gtggtgagcg tgctgaccgt gctgcaccag gattggctga     3900

acggaaagga gtacaagtgc aaggtgagca acaaggccct gccagcccca atcgagaaga     3960

ccatcagcaa ggccaaggga cagccacggg agccacaggt gtacaccctg ccaccaagcc     4020

gggatgagct gaccaagaac caggtgagcc tgacctgcct ggtgaaggga ttctacccaa     4080

gcgatatcgc cgtggagtgg gagagcaacg gacagccaga gaacaactac aagaccaccc     4140

caccagtgct ggatagcgac ggaagcttct tcctgtacag caagctgacc gtggataaga     4200

gccggtggca gcagggaaac gtgttcagct gcagcgtgat gcacgaggcc ctgcacaacc     4260

actacaccca gaagagcctg agcctgagcc caggaaagtg ataaagcggc cgcggtacct     4320

ctagagtcga cccgggcggc ctcgaggacg gggtgaacta cgcctgagga tccgatcttt     4380

ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct     4440

aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg     4500

gaagcaattc gttgatctga atttcgacca cccataatac ccattaccct ggtagataag     4560

tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc     4620

tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc     4680

tttgcccggg cggcctcagt gagcgagcga gcgcgcag                             4718


<210>  16
<211>  736
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AAV hu68 vp1

<400>  16

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Val Gly Ile Gly 
145                 150                 155                 160 


Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 
            260                 265                 270         


Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 
        275                 280                 285             


Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 
    290                 295                 300                 


Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 
305                 310                 315                 320 


Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 
                325                 330                 335     


Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 
            340                 345                 350         


Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 
        355                 360                 365             


Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 
    370                 375                 380                 


Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 
385                 390                 395                 400 


Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 
                405                 410                 415     


Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 
            420                 425                 430         


Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 
        435                 440                 445             


Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser 
    450                 455                 460                 


Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro 
465                 470                 475                 480 


Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn 
                485                 490                 495     


Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn 
            500                 505                 510         


Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys 
        515                 520                 525             


Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly 
    530                 535                 540                 


Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile 
545                 550                 555                 560 


Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser 
                565                 570                 575     


Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln 
            580                 585                 590         


Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln 
        595                 600                 605             


Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 
    610                 615                 620                 


Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met 
625                 630                 635                 640 


Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 
                645                 650                 655     


Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr 
            660                 665                 670         


Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 
        675                 680                 685             


Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn 
    690                 695                 700                 


Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val 
705                 710                 715                 720 


Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
                725                 730                 735     


<210>  17
<211>  736
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human adeno-associated virus 9 vp1

<400>  17

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly 
145                 150                 155                 160 


Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 
            260                 265                 270         


Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 
        275                 280                 285             


Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 
    290                 295                 300                 


Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 
305                 310                 315                 320 


Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 
                325                 330                 335     


Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 
            340                 345                 350         


Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 
        355                 360                 365             


Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 
    370                 375                 380                 


Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 
385                 390                 395                 400 


Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 
                405                 410                 415     


Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 
            420                 425                 430         


Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 
        435                 440                 445             


Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser 
    450                 455                 460                 


Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro 
465                 470                 475                 480 


Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn 
                485                 490                 495     


Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn 
            500                 505                 510         


Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys 
        515                 520                 525             


Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly 
    530                 535                 540                 


Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile 
545                 550                 555                 560 


Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser 
                565                 570                 575     


Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln 
            580                 585                 590         


Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln 
        595                 600                 605             


Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 
    610                 615                 620                 


Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met 
625                 630                 635                 640 


Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 
                645                 650                 655     


Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr 
            660                 665                 670         


Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 
        675                 680                 685             


Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn 
    690                 695                 700                 


Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val 
705                 710                 715                 720 


Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
                725                 730                 735     


<210>  18
<211>  2211
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAVhu68 vp1 coding sequence

<400>  18
atggctgccg atggttatct tccagattgg ctcgaggaca acctcagtga aggcattcgc       60

gagtggtggg ctttgaaacc tggagcccct caacccaagg caaatcaaca acatcaagac      120

aacgctcggg gtcttgtgct tccgggttac aaataccttg gacccggcaa cggactcgac      180

aagggggagc cggtcaacga agcagacgcg gcggccctcg agcacgacaa ggcctacgac      240

cagcagctca aggccggaga caacccgtac ctcaagtaca accacgccga cgccgagttc      300

caggagcggc tcaaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag      360

gccaaaaaga ggcttcttga acctcttggt ctggttgagg aagcggctaa gacggctcct      420

ggaaagaaga ggcctgtaga gcagtctcct caggaaccgg actcctccgt gggtattggc      480

aaatcgggtg cacagcccgc taaaaagaga ctcaatttcg gtcagactgg cgacacagag      540

tcagtccccg accctcaacc aatcggagaa cctcccgcag ccccctcagg tgtgggatct      600

cttacaatgg cttcaggtgg tggcgcacca gtggcagaca ataacgaagg tgccgatgga      660

gtgggtagtt cctcgggaaa ttggcattgc gattcccaat ggctggggga cagagtcatc      720

accaccagca cccgaacctg ggccctgccc acctacaaca atcacctcta caagcaaatc      780

tccaacagca catctggagg atcttcaaat gacaacgcct acttcggcta cagcaccccc      840

tgggggtatt ttgacttcaa cagattccac tgccacttct caccacgtga ctggcaaaga      900

ctcatcaaca acaactgggg attccggcct aagcgactca acttcaagct cttcaacatt      960

caggtcaaag aggttacgga caacaatgga gtcaagacca tcgctaataa ccttaccagc     1020

acggtccagg tcttcacgga ctcagactat cagctcccgt acgtgctcgg gtcggctcac     1080

gagggctgcc tcccgccgtt cccagcggac gttttcatga ttcctcagta cgggtatcta     1140

acgcttaatg atggaagcca agccgtgggt cgttcgtcct tttactgcct ggaatatttc     1200

ccgtcgcaaa tgctaagaac gggtaacaac ttccagttca gctacgagtt tgagaacgta     1260

cctttccata gcagctatgc tcacagccaa agcctggacc gactcatgaa tccactcatc     1320

gaccaatact tgtactatct ctcaaagact attaacggtt ctggacagaa tcaacaaacg     1380

ctaaaattca gtgtggccgg acccagcaac atggctgtcc agggaagaaa ctacatacct     1440

ggacccagct accgacaaca acgtgtctca accactgtga ctcaaaacaa caacagcgaa     1500

tttgcttggc ctggagcttc ttcttgggct ctcaatggac gtaatagctt gatgaatcct     1560

ggacctgcta tggccagcca caaagaagga gaggaccgtt tctttccttt gtctggatct     1620

ttaatttttg gcaaacaagg aactggaaga gacaacgtgg atgcggacaa agtcatgata     1680

accaacgaag aagaaattaa aactaccaac ccagtagcaa cggagtccta tggacaagtg     1740

gccacaaacc accagagtgc ccaagcacag gcgcagaccg gctgggttca aaaccaagga     1800

atacttccgg gtatggtttg gcaggacaga gatgtgtacc tgcaaggacc catttgggcc     1860

aaaattcctc acacggacgg caactttcac ccttctccgc tgatgggagg gtttggaatg     1920

aagcacccgc ctcctcagat cctcatcaaa aacacacctg tacctgcgga tcctccaacg     1980

gctttcaaca aggacaagct gaactctttc atcacccagt attctactgg ccaagtcagc     2040

gtggagattg agtgggagct gcagaaggaa aacagcaagc gctggaaccc ggagatccag     2100

tacacttcca actattacaa gtctaataat gttgaatttg ctgttaatac tgaaggtgtt     2160

tattctgaac cccgccccat tggcaccaga tacctgactc gtaatctgta a              2211


<210>  19
<211>  5351
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector genome encoding four immunolobobulin domains with UbC 
       promoter and SV40 polyA


<220>
<221>  repeat_region
<222>  (17)..(146)
<223>  AAV2-5' ITR

<220>
<221>  promoter
<222>  (207)..(1433)
<223>  UbC promoter, including UB fow priming site

<220>
<221>  Intron
<222>  (1527)..(1659)
<223>  chimeric intron

<220>
<221>  5'UTR
<222>  (1726)..(1773)
<223>  c-myc 5'UTR

<220>
<221>  misc_feature
<222>  (1774)..(1779)
<223>  kozak

<220>
<221>  misc_feature
<222>  (1780)..(1839)
<223>  hm IL2 leader

<220>
<221>  misc_feature
<222>  (1840)..(2111)
<223>  coding region for the first immunoglobulin region

<220>
<221>  misc_binding
<222>  (2212)..(2241)
<223>  linker L1

<220>
<221>  misc_feature
<222>  (2242)..(2610)
<223>  coding sequence for the second immunoglobulin region

<220>
<221>  misc_binding
<222>  (2611)..(2640)
<223>  linker L2

<220>
<221>  misc_feature
<222>  (2641)..(3303)
<223>  Fc1

<220>
<221>  misc_binding
<222>  (3304)..(3315)
<223>  furin

<220>
<221>  misc_binding
<222>  (3316)..(3387)
<223>  2A

<220>
<221>  misc_feature
<222>  (3388)..(3447)
<223>  hm1 IL2 leader

<220>
<221>  misc_feature
<222>  (3448)..(3834)
<223>  coding sequence for the third immunoglobulin region

<220>
<221>  misc_binding
<222>  (3835)..(3864)
<223>  linker sequence L3

<220>
<221>  misc_feature
<222>  (3865)..(4212)
<223>  coding sequence for the fourth immunoglobulin region

<220>
<221>  misc_binding
<222>  (4213)..(4242)
<223>  linker L4

<220>
<221>  misc_feature
<222>  (4243)..(4908)
<223>  Fc2

<220>
<221>  polyA_signal
<222>  (4926)..(5157)
<223>  SV40 late polyA signal

<220>
<221>  repeat_region
<222>  (5222)..(5351)
<223>  AAV2 - 3' ITR

<400>  19
taaggcctta attaggctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg       60

gcgtcgggcg acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt      120

ggccaactcc atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc      180

tacgtagcca tgctctagga agatctggcc tccgcgccgg gttttggcgc ctcccgcggg      240

cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagga gcgtcctgat      300

ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc      360

cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc      420

tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga      480

tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta      540

gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac      600

ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg      660

ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc      720

cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg      780

aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg      840

caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg      900

ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt      960

tgtcgtctgt tgcgggggcg gcagttatgc ggtgccgttg ggcagtgcac ccgtaccttt     1020

gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg     1080

tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg     1140

ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa     1200

gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc     1260

cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct     1320

tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc     1380

gctaaattct ggccgttttt ggcttttttg ttagacgaag ctttattgcg gtagtttatc     1440

acagttaaat tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca     1500

gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag     1560

accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta     1620

ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt     1680

acagctctta aggctagagt acttaatacg actcactata ggctagggca ctttgcactg     1740

gaacttacaa cacccgagca aggacgcgac tctgccacca tgtaccggat gcagctgctg     1800

agctgcatcg ccctgagcct ggccctggtg accaacagcc aggtgcagct ggtggagagc     1860

ggaggaggag tggtgcagcc aggaggaagc ctgcggctga gctgcgccgc cagcatcagc     1920

atcttcgata tctacgccat ggattggtac cggcaggccc caggaaagca gcgggagctg     1980

gtggccgtga gcttccggga cggaagcacc tactacgccg atagcgtgaa gggacggttc     2040

accatcagcc gggataacag caagaacacc ctgtacctgc agatgaacag cctgcgggcc     2100

gaggataccg ccgtgtacta ctgccacgtg agcctgtacc gggatccact gggagtggcc     2160

ggaggaatcg gagtgtactg gggacaggga accctggtga ccgtgagcag cggaggagga     2220

ggaagcggag gaggaggaag cgaggtgcag ctgctggaga gcggaggagg actggtgcag     2280

ccaggaggaa gcctgcgact gagctgtgca gcaagcggac gaacctacgc aatgggctgg     2340

ttccgacagg caccaggaaa ggagcgagag ttcgtggcag caatcaacgc actgggaacc     2400

cgaacctact acgcagacag cgtgaaggga cgattcacca tcagccgaga caacagcaag     2460

aacaccctgt acctgcagat gaacagcctg cgagcagagg acaccgcagt gtactactgt     2520

accgcacagg gacagtggcg agcagcacca gtggcagtgg cagcagagta cgagttctgg     2580

ggacagggaa ccctggtgac cgtgagcagc ggaggaggag gatctggagg aggaggatct     2640

tgtccacctt gtccagcacc agagctgctg ggaggaccaa gcgtgttcct gttcccacca     2700

aagccaaagg acaccctgat gatcagccga accccagagg tgacctgtgt ggtggtggac     2760

gtgagccatg aggacccaga ggtgaagttc aactggtacg tggacggagt ggaggtgcat     2820

aacgcaaaga ccaagccacg agaggagcag tacaacagca cctaccgagt ggtgagcgtg     2880

ctgaccgtgc tgcatcagga ctggctgaac ggaaaggagt acaagtgtaa ggtgagcaac     2940

aaggcactgc cagcaccaat cgagaagacc atcagcaagg caaagggaca gccacgagag     3000

ccacaggtgt acaccctgcc accaagccga gacgagctga ccaagaacca ggtgagcctg     3060

acctgtctgg tgaagggatt ctacccaagc gacatcgcag tggagtggga gagcaacgga     3120

cagccagaga acaactacaa gaccacccca ccagtgctgg acagcgacgg aagcttcttc     3180

ctgtacagca agctgaccgt ggacaagagc cggtggcagc agggaaacgt gttcagctgt     3240

agcgtgatgc acgaggcact gcataaccat tacacccaga agagcctgag cctgagccca     3300

ggacggaagc ggcgggcccc agtgaagcag accctgaact tcgatctgct gaagctggcc     3360

ggagacgtgg agagcaaccc aggaccaatg taccggatgc agctgctgct gctgatcgcc     3420

ctgagcctgg ccctggtgac caacagcgaa gtgcagctgc tggaaagcgg cggcggcctg     3480

gtgcagcccg gcggcagcct gcggctgagc tgtgctgcta gcggctttac cctggaaaac     3540

aaggctattg gctggtttcg gcaggctccc ggcaaggaac gggaaggcgt gctgtgtatt     3600

agcaagagcg gcagctggac ctattatgct gacagcgtga agggccggtt taccattagc     3660

cgggacaaca gcaagaacac cgtgtatctg cagatgaaca gcctgcggcc cgaagacacc     3720

gctgtgtatt attgtgctac caccaccgct ggcggcggcc tgtgttggga cggcaccacc     3780

tttagccggc tggctagcag ctggggccag ggcaccctgg tgaccgtgag cagcggagga     3840

ggaggatccg gaggaggagg atccgaggtg cagctggtgg agtcaggagg aggactggtg     3900

cagcccggag gatcactgag actgtcctgc gctgcttcag gattcacctt ctcaacctcc     3960

tggatgtatt ggctgagaca ggctcccgga aaaggactgg agtgggtgtc agtgatcaac     4020

accgacggag gaacctatta tgctgattca gtgaaaggaa gattcaccat ctcaagagat     4080

aactcaaaaa acaccctgta tctgcagatg aactcactga gagctgagga taccgctgtg     4140

tattattgcg ctaaagattg gggaggaccc gagcccacca gaggacaggg aaccctggtg     4200

accgtgtcat caggaggagg aggatcagga ggaggaggat cctgcccacc ttgcccagcc     4260

ccagagctgc tgggaggacc aagcgtgttc ctgttcccac caaagccaaa ggataccctg     4320

atgatcagcc ggaccccaga ggtgacctgc gtggtggtgg atgtgagcca cgaggatcca     4380

gaggtgaagt tcaactggta cgtggacgga gtggaggtgc acaacgccaa gaccaagcca     4440

cgggaggagc agtacaacag cacctaccgg gtggtgagcg tgctgaccgt gctgcaccag     4500

gattggctga acggaaagga gtacaagtgc aaggtgagca acaaggccct gccagcccca     4560

atcgagaaga ccatcagcaa ggccaaggga cagccacggg agccacaggt gtacaccctg     4620

ccaccaagcc gggatgagct gaccaagaac caggtgagcc tgacctgcct ggtgaaggga     4680

ttctacccaa gcgatatcgc cgtggagtgg gagagcaacg gacagccaga gaacaactac     4740

aagaccaccc caccagtgct ggatagcgac ggaagcttct tcctgtacag caagctgacc     4800

gtggataaga gccggtggca gcagggaaac gtgttcagct gcagcgtgat gcacgaggcc     4860

ctgcacaacc actacaccca gaagagcctg agcctgagcc caggaaagtg ataaagcggc     4920

cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc     4980

agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta     5040

taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg     5100

gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata     5160

aggatcttcc tagagcatgg ctacgtagat aagtagcatg gcgggttaat cattaactac     5220

aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag     5280

gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag     5340

cgagcgcgca g                                                          5351


<210>  20
<211>  4808
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  vector genome with TBG promoter, BGH polyA


<220>
<221>  repeat_region
<222>  (1)..(130)
<223>  AAV2 - 5' ITR

<220>
<221>  promoter
<222>  (205)..(913)
<223>  thyroxine binding globlulin (TBG) promoter

<220>
<221>  TATA_signal
<222>  (875)..(878)

<220>
<221>  Intron
<222>  (939)..(1071)
<223>  SV40 misc intron

<220>
<221>  5'UTR
<222>  (1096)..(1143)
<223>  c-myc 5'UTR

<220>
<221>  misc_feature
<222>  (1144)..(1149)
<223>  kozak

<220>
<221>  misc_feature
<222>  (1150)..(1209)
<223>  hmIL2 linker

<220>
<221>  misc_feature
<222>  (1210)..(1581)
<223>  coding sequence for the first  immunoglobulin region

<220>
<221>  misc_binding
<222>  (1582)..(1611)
<223>  linker L1

<220>
<221>  misc_feature
<222>  (1612)..(1980)
<223>  coding sequence for the second immunogloblulin region

<220>
<221>  misc_binding
<222>  (1981)..(1995)
<223>  hinge

<220>
<221>  misc_feature
<222>  (1996)..(2658)
<223>  Fc1

<220>
<221>  misc_binding
<222>  (2659)..(2670)
<223>  furin

<220>
<221>  misc_binding
<222>  (2671)..(2742)
<223>  2A

<220>
<221>  misc_feature
<222>  (2743)..(2802)
<223>  hm IL2 leader

<220>
<221>  misc_feature
<222>  (2803)..(3189)
<223>  coding sequence for the third immunoglobulin region

<220>
<221>  misc_binding
<222>  (3190)..(3219)
<223>  linker sequence L3

<220>
<221>  misc_feature
<222>  (3220)..(3567)
<223>  coding sequence for the fourth immunoglobulin region

<220>
<221>  misc_binding
<222>  (3568)..(3582)
<223>  hinge

<220>
<221>  misc_feature
<222>  (3583)..(4248)
<223>  Fc2

<220>
<221>  polyA_signal
<222>  (4377)..(4591)
<223>  bovine growth hormone (BGH) polyA

<220>
<221>  repeat_region
<222>  (4679)..(4808)
<223>  AAV2 - 3'ITR

<400>  20
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct      180

aggaagatcg gaattcgccc ttaagctagc aggttaattt ttaaaaagca gtcaaaagtc      240

caagtggccc ttggcagcat ttactctctc tgtttgctct ggttaataat ctcaggagca      300

caaacattcc agatccaggt taatttttaa aaagcagtca aaagtccaag tggcccttgg      360

cagcatttac tctctctgtt tgctctggtt aataatctca ggagcacaaa cattccagat      420

ccggcgcgcc agggctggaa gctacctttg acatcatttc ctctgcgaat gcatgtataa      480

tttctacaga acctattaga aaggatcacc cagcctctgc ttttgtacaa ctttccctta      540

aaaaactgcc aattccactg ctgtttggcc caatagtgag aactttttcc tgctgcctct      600

tggtgctttt gcctatggcc cctattctgc ctgctgaaga cactcttgcc agcatggact      660

taaacccctc cagctctgac aatcctcttt ctcttttgtt ttacatgaag ggtctggcag      720

ccaaagcaat cactcaaagt tcaaacctta tcattttttg ctttgttcct cttggccttg      780

gttttgtaca tcagctttga aaataccatc ccagggttaa tgctggggtt aatttataac      840

taagagtgct ctagttttgc aatacaggac atgctataaa aatggaaaga tgttgctttc      900

tgagagactg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac      960

aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct     1020

gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccagg     1080

cggccaattc gtctagggca ctttgcactg gaacttacaa cacccgagca aggacgcgac     1140

tctgccacca tgtatcggat gcagctgctg agctgtattg ctctgagcct ggctctggtg     1200

accaacagcc aggtgcagct ggtggaaagc ggcggcggcg tggtgcagcc cggcggcagc     1260

ctgcggctga gctgtgctgc tagcattagc atttttgaca tttacgctat ggactggtat     1320

cggcaggctc ccggcaagca gcgggaactg gtggctgtga gctttcggga cggcagcacc     1380

tattatgctg acagcgtgaa gggccggttt accattagcc gggacaacag caagaacacc     1440

ctgtatctgc agatgaacag cctgcgggct gaagacaccg ctgtgtatta ttgtcatgtg     1500

agcctgtatc gggaccccct gggcgtggct ggcggcattg gcgtgtattg gggccagggc     1560

accctggtga ccgtgagcag cggaggagga ggaagcggag gaggaggaag cgaggtgcag     1620

ctgctggaga gcggaggagg actggtgcag ccaggaggaa gcctgcgact gagctgtgca     1680

gcaagcggac gaacctacgc aatgggctgg ttccgacagg caccaggaaa ggagcgagag     1740

ttcgtggcag caatcaacgc actgggaacc cgaacctact acgcagacag cgtgaaggga     1800

cgattcacca tcagccgaga caacagcaag aacaccctgt acctgcagat gaacagcctg     1860

cgagcagagg acaccgcagt gtactactgt accgcacagg gacagtggcg agcagcacca     1920

gtggcagtgg cagcagagta cgagttctgg ggacagggaa ccctggtgac cgtgagcagc     1980

gacaagaccc atacctgtcc accttgtcca gcaccagagc tgctgggagg accaagcgtg     2040

ttcctgttcc caccaaagcc aaaggacacc ctgatgatca gccgaacccc agaggtgacc     2100

tgtgtggtgg tggacgtgag ccatgaggac ccagaggtga agttcaactg gtacgtggac     2160

ggagtggagg tgcataacgc aaagaccaag ccacgagagg agcagtacaa cagcacctac     2220

cgagtggtga gcgtgctgac cgtgctgcat caggactggc tgaacggaaa ggagtacaag     2280

tgtaaggtga gcaacaaggc actgccagca ccaatcgaga agaccatcag caaggcaaag     2340

ggacagccac gagagccaca ggtgtacacc ctgccaccaa gccgagacga gctgaccaag     2400

aaccaggtga gcctgacctg tctggtgaag ggattctacc caagcgacat cgcagtggag     2460

tgggagagca acggacagcc agagaacaac tacaagacca ccccaccagt gctggacagc     2520

gacggaagct tctttctgta cagcaagctg accgtggaca agagccggtg gcagcaggga     2580

aacgtgttca gctgtagcgt gatgcacgag gcactgcata accattacac ccagaagagc     2640

ctgagcctga gcccaggacg gaagcggcgg gctcccgtga agcagaccct gaactttgac     2700

ctgctgaagc tggctggcga cgtggaaagc aaccccggcc ccatgtatcg gatgcagctg     2760

ctgctgctga ttgctctgag cctggctctg gtgaccaaca gcgaggtgca gctgctggag     2820

agcggaggag gactggtgca gccaggagga agcctgcggc tgagctgcgc cgccagcgga     2880

ttcaccctgg agaacaaggc catcggctgg ttccggcagg ccccaggaaa ggagcgggag     2940

ggagtgctgt gcatcagcaa gagcggaagc tggacctact acgccgatag cgtgaaggga     3000

cggttcacca tcagccggga taacagcaag aacaccgtgt acctgcagat gaacagcctg     3060

cggccagagg ataccgccgt gtactactgc gccaccacca ccgccggagg aggactgtgc     3120

tgggacggaa ccaccttcag ccggctggcc agcagctggg gacagggaac cctggtgacc     3180

gtgagcagcg gaggaggagg atccggagga ggaggatccg aggtgcagct ggtggagtca     3240

ggaggaggac tggtgcagcc cggaggatca ctgagactgt cctgcgctgc ttcaggattc     3300

accttctcaa cctcctggat gtattggctg agacaggctc ccggaaaagg actggagtgg     3360

gtgtcagtga tcaacaccga cggaggaacc tattatgctg attcagtgaa aggaagattc     3420

accatctcaa gagataactc aaaaaacacc ctgtatctgc agatgaactc actgagagct     3480

gaggataccg ctgtgtatta ttgcgctaaa gattggggag gacccgagcc caccagagga     3540

cagggaaccc tggtgaccgt gtcatcagac aagacccata cctgtccccc ctgtcccgct     3600

cccgaactgc tgggcggccc cagcgtgttt ctgtttcccc ccaagcccaa ggacaccctg     3660

atgattagcc ggacccccga agtgacctgt gtggtggtgg acgtgagcca tgaagacccc     3720

gaagtgaagt ttaactggta cgtggacggc gtggaagtgc ataacgctaa gaccaagccc     3780

cgggaagaac agtataacag cacctatcgg gtggtgagcg tgctgaccgt gctgcatcag     3840

gactggctga acggcaagga atataagtgt aaggtgagca acaaggctct gcccgctccc     3900

attgaaaaga ccattagcaa ggctaagggc cagccccggg aaccccaggt gtataccctg     3960

ccccccagcc gggacgaact gaccaagaac caggtgagcc tgacctgtct ggtgaagggc     4020

ttttatccca gcgacattgc tgtggagtgg gaaagcaacg gccagcccga aaacaactat     4080

aagaccaccc cccccgtgct ggacagcgac ggcagctttt ttctgtatag caagctgacc     4140

gtggacaaga gccggtggca gcagggcaac gtgtttagct gtagcgtgat gcacgaagct     4200

ctgcataacc attataccca gaagagcctg agcctgagcc ccggcaagtg ataaagcggc     4260

cgcagcttat ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc     4320

agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa ggatctgcct     4380

cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga     4440

ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt     4500

gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg     4560

attgggaaga caatagcagg catgctgggg actcgagtta agggcgaatt cccgataagg     4620

atcttcctag agcatggcta cgtagataag tagcatggcg ggttaatcat taactacaag     4680

gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc     4740

gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga     4800

gcgcgcag                                                              4808


<210>  21
<211>  2361
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  J211 coding sequence for fusion protein


<220>
<221>  5'UTR
<222>  (1)..(48)
<223>  c-myc 5'UTR

<220>
<221>  misc_feature
<222>  (49)..(54)
<223>  kozak

<220>
<221>  misc_feature
<222>  (55)..(114)
<223>  hmIL2 leader

<220>
<221>  misc_feature
<222>  (115)..(486)
<223>  coding sequence for the first immunoglobulin region

<220>
<221>  misc_binding
<222>  (487)..(516)
<223>  linker sequence L1

<220>
<221>  misc_feature
<222>  (517)..(885)
<223>  coding sequence for the second immunoglobulin region

<220>
<221>  misc_binding
<222>  (886)..(915)
<223>  leader sequence L2

<220>
<221>  misc_feature
<222>  (916)..(1302)
<223>  coding sequence for the third immunoglobulin region

<220>
<221>  misc_binding
<222>  (1303)..(1332)
<223>  linker sequence L3

<220>
<221>  misc_feature
<222>  (1333)..(1680)
<223>  coding sequence for the fourth immunoglobulin region

<220>
<221>  misc_binding
<222>  (1681)..(1695)
<223>  hinge

<220>
<221>  misc_feature
<222>  (1696)..(2361)
<223>  Fc

<400>  21
gggcactttg cactggaact tacaacaccc gagcaaggac gcgactctgc caccatgtat       60

cggatgcagc tgctgagctg tattgctctg agcctggctc tggtgaccaa cagccaggtg      120

cagctggtgg aaagcggcgg cggcgtggtg cagcccggcg gcagcctgcg gctgagctgt      180

gctgctagca ttagcatttt tgacatttac gctatggact ggtatcggca ggctcccggc      240

aagcagcggg aactggtggc tgtgagcttt cgggacggca gcacctatta tgctgacagc      300

gtgaagggcc ggtttaccat tagccgggac aacagcaaga acaccctgta tctgcagatg      360

aacagcctgc gggctgaaga caccgctgtg tattattgtc atgtgagcct gtatcgggac      420

cccctgggcg tggctggcgg cattggcgtg tattggggcc agggcaccct ggtgaccgtg      480

agcagcggag gaggaggaag cggaggagga ggaagcgagg tgcagctgct ggagagcgga      540

ggaggactgg tgcagccagg aggaagcctg cgactgagct gtgcagcaag cggacgaacc      600

tacgcaatgg gctggttccg acaggcacca ggaaaggagc gagagttcgt ggcagcaatc      660

aacgcactgg gaacccgaac ctactacgca gacagcgtga agggacgatt caccatcagc      720

cgagacaaca gcaagaacac cctgtacctg cagatgaaca gcctgcgagc agaggacacc      780

gcagtgtact actgtaccgc acagggacag tggcgagcag caccagtggc agtggcagca      840

gagtacgagt tctggggaca gggaaccctg gtgaccgtga gcagcggagg aggaggatct      900

ggaggaggag gatctgaggt gcagctgctg gagagcggag gaggactggt gcagccagga      960

ggaagcctgc ggctgagctg cgccgccagc ggattcaccc tggagaacaa ggccatcggc     1020

tggttccggc aggccccagg aaaggagcgg gagggagtgc tgtgcatcag caagagcgga     1080

agctggacct actacgccga tagcgtgaag ggacggttca ccatcagccg ggataacagc     1140

aagaacaccg tgtacctgca gatgaacagc ctgcggccag aggataccgc cgtgtactac     1200

tgcgccacca ccaccgccgg aggaggactg tgctgggacg gaaccacctt cagccggctg     1260

gccagcagct ggggacaggg aaccctggtg accgtgagca gcggaggagg aggatccgga     1320

ggaggaggat ccgaggtgca gctggtggag tcaggaggag gactggtgca gcccggagga     1380

tcactgagac tgtcctgcgc tgcttcagga ttcaccttct caacctcctg gatgtattgg     1440

ctgagacagg ctcccggaaa aggactggag tgggtgtcag tgatcaacac cgacggagga     1500

acctattatg ctgattcagt gaaaggaaga ttcaccatct caagagataa ctcaaaaaac     1560

accctgtatc tgcagatgaa ctcactgaga gctgaggata ccgctgtgta ttattgcgct     1620

aaagattggg gaggacccga gcccaccaga ggacagggaa ccctggtgac cgtgtcatca     1680

gacaagaccc atacctgtcc cccctgtccc gctcccgaac tgctgggcgg ccccagcgtg     1740

tttctgtttc cccccaagcc caaggacacc ctgatgatta gccggacccc cgaagtgacc     1800

tgtgtggtgg tggacgtgag ccatgaagac cccgaagtga agtttaactg gtacgtggac     1860

ggcgtggaag tgcataacgc taagaccaag ccccgggaag aacagtataa cagcacctat     1920

cgggtggtga gcgtgctgac cgtgctgcat caggactggc tgaacggcaa ggaatataag     1980

tgtaaggtga gcaacaaggc tctgcccgct cccattgaaa agaccattag caaggctaag     2040

ggccagcccc gggaacccca ggtgtatacc ctgcccccca gccgggacga actgaccaag     2100

aaccaggtga gcctgacctg tctggtgaag ggcttttatc ccagcgacat tgctgtggag     2160

tgggaaagca acggccagcc cgaaaacaac tataagacca ccccccccgt gctggacagc     2220

gacggcagct tttttctgta tagcaagctg accgtggaca agagccggtg gcagcagggc     2280

aacgtgttta gctgtagcgt gatgcacgaa gctctgcata accattatac ccagaagagc     2340

ctgagcctga gccccggcaa g                                               2361


<210>  22
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  22

Ala Ala Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
1               5                   10                  15      


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            20                  25                  30          


Ser Gly Gly Gly Gly Ser 
        35              


<210>  23
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  23

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  24
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  24

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser 
        35  


<210>  25
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  25

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25                  30          


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        35                  40                  45              


Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    50                  55          


<210>  26
<211>  2307
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  J210a coding sequence


<220>
<221>  CDS
<222>  (1)..(2307)

<400>  26
atg tac cga atg cag ctg ctg agc tgt atc gca ctg agc ctg gca ctg         48
Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu           
1               5                   10                  15                

gtg acc aac agc cag gtg cag ctg gtg gag agc gga gga gga gtg gtg         96
Val Thr Asn Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val           
            20                  25                  30                    

cag cca gga gga agc ctg cga ctg agc tgt gca gca agc atc agc atc        144
Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Ile Ser Ile           
        35                  40                  45                        

ttc gac atc tac gca atg gac tgg tac cga cag gca cca gga aag cag        192
Phe Asp Ile Tyr Ala Met Asp Trp Tyr Arg Gln Ala Pro Gly Lys Gln           
    50                  55                  60                            

cga gag ctg gtg gca gtg agc ttc cga gac gga agc acc tac tac gca        240
Arg Glu Leu Val Ala Val Ser Phe Arg Asp Gly Ser Thr Tyr Tyr Ala           
65                  70                  75                  80            

gac agc gtg aag gga cga ttc acc atc agc cga gac aac agc aag aac        288
Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn           
                85                  90                  95                

acc ctg tac ctg cag atg aac agc ctg cga gca gag gac acc gca gtg        336
Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val           
            100                 105                 110                   

tac tac tgt cat gtg agc ctg tac cgg gac cca ctg gga gtg gca gga        384
Tyr Tyr Cys His Val Ser Leu Tyr Arg Asp Pro Leu Gly Val Ala Gly           
        115                 120                 125                       

gga atc gga gtg tac tgg gga cag gga acc ctg gtg acc gtg agc agc        432
Gly Ile Gly Val Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser           
    130                 135                 140                           

gga gga gga gga agc gga gga gga gga agc gaa gtg cag ctg ctg gaa        480
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Leu Glu           
145                 150                 155                 160           

agc ggc ggc ggc ctg gtg cag ccc ggc ggc agc ctg cgg ctg agc tgt        528
Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys           
                165                 170                 175               

gct gct agc ggc cgg acc tac gct atg ggc tgg ttt cgg cag gct ccc        576
Ala Ala Ser Gly Arg Thr Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro           
            180                 185                 190                   

ggc aag gaa cgg gaa ttt gtg gct gct att aac gct ctg ggc acc cgg        624
Gly Lys Glu Arg Glu Phe Val Ala Ala Ile Asn Ala Leu Gly Thr Arg           
        195                 200                 205                       

acc tat tat gct gac agc gtg aag ggc cgg ttt acc att agc cgg gac        672
Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp           
    210                 215                 220                           

aac agc aag aac acc ctg tat ctg cag atg aac agc ctg cgg gct gaa        720
Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu           
225                 230                 235                 240           

gac acc gct gtg tat tat tgt acc gct cag ggc cag tgg cgg gct gct        768
Asp Thr Ala Val Tyr Tyr Cys Thr Ala Gln Gly Gln Trp Arg Ala Ala           
                245                 250                 255               

ccc gtg gct gtg gct gct gaa tac gaa ttt tgg ggc cag ggc acc ctg        816
Pro Val Ala Val Ala Ala Glu Tyr Glu Phe Trp Gly Gln Gly Thr Leu           
            260                 265                 270                   

gtg acc gtg agc agc gga gga gga gga tct gga gga gga gga tct gag        864
Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu           
        275                 280                 285                       

gtg cag ctg ctg gag agc gga gga gga ctg gtg cag cca gga gga agc        912
Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser           
    290                 295                 300                           

ctg cgg ctg agc tgc gcc gcc agc gga ttc acc ctg gag aac aag gcc        960
Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Leu Glu Asn Lys Ala           
305                 310                 315                 320           

atc ggc tgg ttc cgg cag gcc cca gga aag gag cgg gag gga gtg ctg       1008
Ile Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val Leu           
                325                 330                 335               

tgc atc agc aag agc gga agc tgg acc tac tac gcc gat agc gtg aag       1056
Cys Ile Ser Lys Ser Gly Ser Trp Thr Tyr Tyr Ala Asp Ser Val Lys           
            340                 345                 350                   

gga cgg ttc acc atc agc cgg gat aac agc aag aac acc gtg tac ctg       1104
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Val Tyr Leu           
        355                 360                 365                       

cag atg aac agc ctg cgg cca gag gat acc gcc gtg tac tac tgc gcc       1152
Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala           
    370                 375                 380                           

acc acc acc gcc gga gga gga ctg tgc tgg gac gga acc acc ttc agc       1200
Thr Thr Thr Ala Gly Gly Gly Leu Cys Trp Asp Gly Thr Thr Phe Ser           
385                 390                 395                 400           

cgg ctg gcc agc agc tgg gga cag gga acc ctg gtg acc gtg agc agc       1248
Arg Leu Ala Ser Ser Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser           
                405                 410                 415               

gga gga gga gga tcc gga gga gga gga tcc gag gtg cag ctg gtg gag       1296
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu           
            420                 425                 430                   

tca gga gga gga ctg gtg cag ccc gga gga tca ctg aga ctg tcc tgc       1344
Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys           
        435                 440                 445                       

gct gct tca gga ttc acc ttc tca acc tcc tgg atg tat tgg ctg aga       1392
Ala Ala Ser Gly Phe Thr Phe Ser Thr Ser Trp Met Tyr Trp Leu Arg           
    450                 455                 460                           

cag gct ccc gga aaa gga ctg gag tgg gtg tca gtg atc aac acc gac       1440
Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Val Ile Asn Thr Asp           
465                 470                 475                 480           

gga gga acc tat tat gct gat tca gtg aaa gga aga ttc acc atc tca       1488
Gly Gly Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser           
                485                 490                 495               

aga gat aac tca aaa aac acc ctg tat ctg cag atg aac tca ctg aga       1536
Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg           
            500                 505                 510                   

gct gag gat acc gct gtg tat tat tgc gct aaa gat tgg gga gga ccc       1584
Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Lys Asp Trp Gly Gly Pro           
        515                 520                 525                       

gag ccc acc aga gga cag gga acc ctg gtg acc gtg tca tca gac aag       1632
Glu Pro Thr Arg Gly Gln Gly Thr Leu Val Thr Val Ser Ser Asp Lys           
    530                 535                 540                           

acc cat acc tgt cca cct tgt cca gca cca gag ctg ctg gga gga cca       1680
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro           
545                 550                 555                 560           

agc gtg ttc ctg ttc cca cca aag cca aag gac acc ctg atg atc agc       1728
Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser           
                565                 570                 575               

cga acc cca gag gtg acc tgt gtg gtg gtg gac gtg agc cat gag gac       1776
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp           
            580                 585                 590                   

cca gag gtg aag ttc aac tgg tac gtg gac gga gtg gag gtg cat aac       1824
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn           
        595                 600                 605                       

gca aag acc aag cca cga gag gag cag tac aac agc acc tac cga gtg       1872
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val           
    610                 615                 620                           

gtg agc gtg ctg acc gtg ctg cat cag gac tgg ctg aac gga aag gag       1920
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu           
625                 630                 635                 640           

tac aag tgt aag gtg agc aac aag gca ctg cca gca cca atc gag aag       1968
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys           
                645                 650                 655               

acc atc agc aag gca aag gga cag cca cga gag cca cag gtg tac acc       2016
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr           
            660                 665                 670                   

ctg cca cca agc cga gac gag ctg acc aag aac cag gtg agc ctg acc       2064
Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr           
        675                 680                 685                       

tgt ctg gtg aag gga ttc tac cca agc gac atc gca gtg gag tgg gag       2112
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu           
    690                 695                 700                           

agc aac gga cag cca gag aac aac tac aag acc acc cca cca gtg ctg       2160
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu           
705                 710                 715                 720           

gac agc gac gga agc ttc ttc ctg tac agc aag ctg acc gtg gac aag       2208
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys           
                725                 730                 735               

agc cgg tgg cag cag gga aac gtg ttc agc tgt agc gtg atg cac gag       2256
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu           
            740                 745                 750                   

gca ctg cat aac cat tac acc cag aag agc ctg agc ctg agc cca gga       2304
Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly           
        755                 760                 765                       

aag                                                                   2307
Lys                                                                       
                                                                          


<210>  27
<211>  769
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  27

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Ile Ser Ile 
        35                  40                  45              


Phe Asp Ile Tyr Ala Met Asp Trp Tyr Arg Gln Ala Pro Gly Lys Gln 
    50                  55                  60                  


Arg Glu Leu Val Ala Val Ser Phe Arg Asp Gly Ser Thr Tyr Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
                85                  90                  95      


Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys His Val Ser Leu Tyr Arg Asp Pro Leu Gly Val Ala Gly 
        115                 120                 125             


Gly Ile Gly Val Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Leu Glu 
145                 150                 155                 160 


Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys 
                165                 170                 175     


Ala Ala Ser Gly Arg Thr Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro 
            180                 185                 190         


Gly Lys Glu Arg Glu Phe Val Ala Ala Ile Asn Ala Leu Gly Thr Arg 
        195                 200                 205             


Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
    210                 215                 220                 


Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu 
225                 230                 235                 240 


Asp Thr Ala Val Tyr Tyr Cys Thr Ala Gln Gly Gln Trp Arg Ala Ala 
                245                 250                 255     


Pro Val Ala Val Ala Ala Glu Tyr Glu Phe Trp Gly Gln Gly Thr Leu 
            260                 265                 270         


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
        275                 280                 285             


Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser 
    290                 295                 300                 


Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Leu Glu Asn Lys Ala 
305                 310                 315                 320 


Ile Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val Leu 
                325                 330                 335     


Cys Ile Ser Lys Ser Gly Ser Trp Thr Tyr Tyr Ala Asp Ser Val Lys 
            340                 345                 350         


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Val Tyr Leu 
        355                 360                 365             


Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
    370                 375                 380                 


Thr Thr Thr Ala Gly Gly Gly Leu Cys Trp Asp Gly Thr Thr Phe Ser 
385                 390                 395                 400 


Arg Leu Ala Ser Ser Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
                405                 410                 415     


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu 
            420                 425                 430         


Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys 
        435                 440                 445             


Ala Ala Ser Gly Phe Thr Phe Ser Thr Ser Trp Met Tyr Trp Leu Arg 
    450                 455                 460                 


Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Val Ile Asn Thr Asp 
465                 470                 475                 480 


Gly Gly Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser 
                485                 490                 495     


Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg 
            500                 505                 510         


Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Lys Asp Trp Gly Gly Pro 
        515                 520                 525             


Glu Pro Thr Arg Gly Gln Gly Thr Leu Val Thr Val Ser Ser Asp Lys 
    530                 535                 540                 


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
545                 550                 555                 560 


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
                565                 570                 575     


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
            580                 585                 590         


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
        595                 600                 605             


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
    610                 615                 620                 


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
625                 630                 635                 640 


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
                645                 650                 655     


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            660                 665                 670         


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr 
        675                 680                 685             


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    690                 695                 700                 


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
705                 710                 715                 720 


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
                725                 730                 735     


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
            740                 745                 750         


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        755                 760                 765             


Lys 
    


<210>  28
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer prm504

<400>  28
gctgcgycaa ctggaccaat gagaac                                            26


<210>  29
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer prm505

<400>  29
cgcagagacc aaagttcaac tgaaacga                                          28


<210>  30
<211>  772
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of MD3606


<220>
<221>  MISC_FEATURE
<222>  (24)..(147)
<223>  The first immunoglobulin region with I110M

<220>
<221>  MISC_FEATURE
<222>  (158)..(280)
<223>  The second immunoglobulin region

<220>
<221>  MISC_FEATURE
<222>  (291)..(419)
<223>  The third immunoglobulin region

<220>
<221>  MISC_FEATURE
<222>  (430)..(485)
<223>  The fourth immunoglobulin region

<220>
<221>  MISC_FEATURE
<222>  (551)..(772)
<223>  Fc

<400>  30

Glu Phe Ala Thr Met Ala Trp Val Trp Thr Leu Leu Phe Leu Met Ala 
1               5                   10                  15      


Ala Ala Gln Ser Ile Gln Ala Gln Val Gln Leu Val Glu Ser Gly Gly 
            20                  25                  30          


Gly Val Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
        35                  40                  45              


Ile Ser Ile Phe Asp Ile Tyr Ala Met Asp Trp Tyr Arg Gln Ala Pro 
    50                  55                  60                  


Gly Lys Gln Arg Glu Leu Val Ala Val Ser Phe Arg Asp Gly Ser Thr 
65                  70                  75                  80  


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys His Val Ser Leu Tyr Arg Asp Pro Leu Gly 
        115                 120                 125             


Val Ala Gly Gly Met Gly Val Tyr Trp Gly Gln Gly Thr Leu Val Thr 
    130                 135                 140                 


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln 
145                 150                 155                 160 


Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg 
                165                 170                 175     


Leu Ser Cys Ala Ala Ser Gly Arg Thr Tyr Ala Met Gly Trp Phe Arg 
            180                 185                 190         


Gln Ala Pro Gly Lys Glu Arg Glu Phe Val Ala Ala Ile Asn Ala Leu 
        195                 200                 205             


Gly Thr Arg Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile 
    210                 215                 220                 


Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu 
225                 230                 235                 240 


Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Thr Ala Gln Gly Gln Trp 
                245                 250                 255     


Arg Ala Ala Pro Val Ala Val Ala Ala Glu Tyr Glu Phe Trp Gly Gln 
            260                 265                 270         


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        275                 280                 285             


Gly Ser Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro 
    290                 295                 300                 


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Leu Glu 
305                 310                 315                 320 


Asn Lys Ala Ile Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
                325                 330                 335     


Gly Val Leu Cys Ile Ser Lys Ser Gly Ser Trp Thr Tyr Tyr Ala Asp 
            340                 345                 350         


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr 
        355                 360                 365             


Val Tyr Leu Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr 
    370                 375                 380                 


Tyr Cys Ala Thr Thr Thr Ala Gly Gly Gly Leu Cys Trp Asp Gly Thr 
385                 390                 395                 400 


Thr Phe Ser Arg Leu Ala Ser Ser Trp Gly Gln Gly Thr Leu Val Thr 
                405                 410                 415     


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln 
            420                 425                 430         


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg 
        435                 440                 445             


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Ser Trp Met Tyr 
    450                 455                 460                 


Trp Leu Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Val Ile 
465                 470                 475                 480 


Asn Thr Asp Gly Gly Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe 
                485                 490                 495     


Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn 
            500                 505                 510         


Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Lys Asp Trp 
        515                 520                 525             


Gly Gly Pro Glu Pro Thr Arg Gly Gln Gly Thr Leu Val Thr Val Ser 
    530                 535                 540                 


Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
545                 550                 555                 560 


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                565                 570                 575     


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
            580                 585                 590         


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
        595                 600                 605             


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
    610                 615                 620                 


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
625                 630                 635                 640 


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
                645                 650                 655     


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
            660                 665                 670         


Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
        675                 680                 685             


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
    690                 695                 700                 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
705                 710                 715                 720 


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
                725                 730                 735     


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
            740                 745                 750         


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
        755                 760                 765             


Ser Pro Gly Lys 
    770         


<210>  31
<211>  4690
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAV vector genome AAV.CB7.hJAB Fc


<220>
<221>  repeat_region
<222>  (1)..(130)
<223>  5' IRT

<220>
<221>  promoter
<222>  (198)..(579)
<223>  CMV IE promoter

<220>
<221>  enhancer
<222>  (279)..(538)
<223>  C4 enhancer with 2 mismatches

<220>
<221>  promoter
<222>  (582)..(862)
<223>  CB promoter

<220>
<221>  TATA_signal
<222>  (836)..(839)
<223>  TATA

<220>
<221>  Intron
<222>  (956)..(1928)
<223>  chicken beta-actin intron

<220>
<221>  misc_feature
<222>  (1941)..(4259)
<223>  coding sequence for MD3606

<220>
<221>  polyA_signal
<222>  (4346)..(4472)
<223>  Rabbit globin poly A

<220>
<221>  repeat_region
<222>  (4561)..(4690)
<223>  3' ITR

<400>  31
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg      180

atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat      240

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      300

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      360

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      420

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      480

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc      540

tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac      600

gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat      660

tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg      720

ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca      780

gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa      840

aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc      900

cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag      960

cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt     1020

ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg     1080

gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc     1140

gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt     1200

gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa     1260

caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt     1320

cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg     1380

tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca     1440

ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg     1500

cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt     1560

atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa     1620

atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg     1680

caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc     1740

tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc     1800

ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc     1860

cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt     1920

tggcaaagaa ttcgctagat gaattcgcca ccatggcttg ggtgtggacc ttgctattcc     1980

tgatggcagc tgcccaaagt atacaggccc aggtgcagct ggtggagagc ggcggcggcg     2040

tggtgcagcc cggcggcagc ctgcgcctga gctgcgccgc cagcatcagc atcttcgaca     2100

tctacgccat ggactggtac cgccaggccc ccggcaagca gcgcgagctg gtggccgtga     2160

gcttccgcga cggcagcacc tactacgccg acagcgtgaa gggccgcttc accatcagcc     2220

gcgacaacag caagaacacc ctgtacctgc agatgaacag cctgcgcgcc gaggacaccg     2280

ccgtgtacta ctgccacgtg agcctgtacc gcgaccccct gggcgtggcc ggcggcatgg     2340

gcgtgtactg gggccagggc accctggtga ccgtgagcag cggcggcggc ggcagcggcg     2400

gcggcggcag cgaggtgcag ctgctggaga gcggcggcgg cctggtgcag cccggcggca     2460

gcctgcgcct gagctgcgcc gccagcggcc gcacctacgc catgggctgg ttccgccagg     2520

cccccggcaa ggagcgcgag ttcgtggccg ccatcaacgc cctgggcacc cgcacctact     2580

acgccgacag cgtgaagggc cgcttcacca tcagccgcga caacagcaag aacaccctgt     2640

acctgcagat gaacagcctg cgcgccgagg acaccgccgt gtactactgc accgcccagg     2700

gccagtggcg cgccgccccc gtggccgtgg ccgccgagta cgagttctgg ggccagggca     2760

ccctggtgac cgtgagcagc ggcggcggcg gcagcggcgg cggcggcagc gaggtgcagc     2820

tgctggagag cggcggcggc ctggtgcagc ccggcggcag cctgcgcctg agctgcgccg     2880

ccagcggctt caccctggag aacaaggcca tcggctggtt ccgccaggcc cccggcaagg     2940

agcgcgaggg cgtgctgtgc atcagcaaga gcggcagctg gacctactac gccgacagcg     3000

tgaagggccg cttcaccatc agccgcgaca acagcaagaa caccgtgtac ctgcagatga     3060

acagcctgcg ccccgaggac accgccgtgt actactgcgc caccaccacc gccggcggcg     3120

gcctgtgctg ggacggcacc accttcagcc gcctggccag cagctggggc cagggcaccc     3180

tggtgaccgt gagcagcggc ggcggcggca gcggcggcgg cggcagcgag gtgcagctgg     3240

tggagagcgg cggcggcctg gtgcagcccg gcggcagcct gcgcctgagc tgcgccgcca     3300

gcggcttcac cttcagcacc agctggatgt actggctgcg ccaggccccc ggcaagggcc     3360

tggagtgggt gagcgtgatc aacaccgacg gcggcaccta ctacgccgac agcgtgaagg     3420

gccgcttcac catcagccgc gacaacagca agaacaccct gtacctgcag atgaacagcc     3480

tgcgcgccga ggacaccgcc gtgtactact gcgccaagga ctggggcggc cccgagccca     3540

cccgcggcca gggcaccctg gtgaccgtga gcagcgacaa aactcacaca tgcccaccgt     3600

gcccagcacc tgaactcctg gggggaccgt cagtcttcct cttcccccca aaacccaagg     3660

acaccctcat gatctcccgg acccctgagg tcacatgcgt ggtggtggac gtgagccacg     3720

aagaccctga ggtcaagttc aactggtacg tggacggcgt ggaggtgcat aatgccaaga     3780

caaagccgcg ggaggagcag tacaacagca cgtaccgggt ggtcagcgtc ctcaccgtcc     3840

tgcaccagga ctggctgaat ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc     3900

cagcccccat cgagaaaacc atctccaaag ccaaagggca gccccgagaa ccacaggtgt     3960

acaccctgcc cccatcccgg gatgagctga ccaagaacca ggtcagcctg acctgcctgg     4020

tcaaaggctt ctatcccagc gacatcgccg tggagtggga gagcaatggg cagccggaga     4080

acaactacaa gaccacgcct cccgtgctgg actccgacgg ctccttcttc ctctacagca     4140

agctcaccgt ggacaagagc aggtggcagc aggggaacgt cttctcatgc tccgtgatgc     4200

atgaggctct gcacaaccac tacacgcaga agagcctctc cctgtctccg ggtaaatgag     4260

aattcatcgg atcccgggcc cgtcgactgc agaggcctgc atgcaagctt ggctcgagga     4320

cggggtgaac tacgcctgag gatccgatct ttttccctct gccaaaaatt atggggacat     4380

catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat     4440

agtgtgttgg aattttttgt gtctctcact cggaagcaat tcgttgatct gaatttcgac     4500

cacccataat acccattacc ctggtagata agtagcatgg cgggttaatc attaactaca     4560

aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg     4620

ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc     4680

gagcgcgcag                                                            4690


<210>  32
<211>  2319
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  coding sequence of MD3606

<400>  32
gaattcgcca ccatggcttg ggtgtggacc ttgctattcc tgatggcagc tgcccaaagt       60

atacaggccc aggtgcagct ggtggagagc ggcggcggcg tggtgcagcc cggcggcagc      120

ctgcgcctga gctgcgccgc cagcatcagc atcttcgaca tctacgccat ggactggtac      180

cgccaggccc ccggcaagca gcgcgagctg gtggccgtga gcttccgcga cggcagcacc      240

tactacgccg acagcgtgaa gggccgcttc accatcagcc gcgacaacag caagaacacc      300

ctgtacctgc agatgaacag cctgcgcgcc gaggacaccg ccgtgtacta ctgccacgtg      360

agcctgtacc gcgaccccct gggcgtggcc ggcggcatgg gcgtgtactg gggccagggc      420

accctggtga ccgtgagcag cggcggcggc ggcagcggcg gcggcggcag cgaggtgcag      480

ctgctggaga gcggcggcgg cctggtgcag cccggcggca gcctgcgcct gagctgcgcc      540

gccagcggcc gcacctacgc catgggctgg ttccgccagg cccccggcaa ggagcgcgag      600

ttcgtggccg ccatcaacgc cctgggcacc cgcacctact acgccgacag cgtgaagggc      660

cgcttcacca tcagccgcga caacagcaag aacaccctgt acctgcagat gaacagcctg      720

cgcgccgagg acaccgccgt gtactactgc accgcccagg gccagtggcg cgccgccccc      780

gtggccgtgg ccgccgagta cgagttctgg ggccagggca ccctggtgac cgtgagcagc      840

ggcggcggcg gcagcggcgg cggcggcagc gaggtgcagc tgctggagag cggcggcggc      900

ctggtgcagc ccggcggcag cctgcgcctg agctgcgccg ccagcggctt caccctggag      960

aacaaggcca tcggctggtt ccgccaggcc cccggcaagg agcgcgaggg cgtgctgtgc     1020

atcagcaaga gcggcagctg gacctactac gccgacagcg tgaagggccg cttcaccatc     1080

agccgcgaca acagcaagaa caccgtgtac ctgcagatga acagcctgcg ccccgaggac     1140

accgccgtgt actactgcgc caccaccacc gccggcggcg gcctgtgctg ggacggcacc     1200

accttcagcc gcctggccag cagctggggc cagggcaccc tggtgaccgt gagcagcggc     1260

ggcggcggca gcggcggcgg cggcagcgag gtgcagctgg tggagagcgg cggcggcctg     1320

gtgcagcccg gcggcagcct gcgcctgagc tgcgccgcca gcggcttcac cttcagcacc     1380

agctggatgt actggctgcg ccaggccccc ggcaagggcc tggagtgggt gagcgtgatc     1440

aacaccgacg gcggcaccta ctacgccgac agcgtgaagg gccgcttcac catcagccgc     1500

gacaacagca agaacaccct gtacctgcag atgaacagcc tgcgcgccga ggacaccgcc     1560

gtgtactact gcgccaagga ctggggcggc cccgagccca cccgcggcca gggcaccctg     1620

gtgaccgtga gcagcgacaa aactcacaca tgcccaccgt gcccagcacc tgaactcctg     1680

gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat gatctcccgg     1740

acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga ggtcaagttc     1800

aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg ggaggagcag     1860

tacaacagca cgtaccgggt ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat     1920

ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc cagcccccat cgagaaaacc     1980

atctccaaag ccaaagggca gccccgagaa ccacaggtgt acaccctgcc cccatcccgg     2040

gatgagctga ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt ctatcccagc     2100

gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa gaccacgcct     2160

cccgtgctgg actccgacgg ctccttcttc ctctacagca agctcaccgt ggacaagagc     2220

aggtggcagc aggggaacgt cttctcatgc tccgtgatgc atgaggctct gcacaaccac     2280

tacacgcaga agagcctctc cctgtctccg ggtaaatga                            2319


<210>  33
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spacer

<400>  33
atgacttaaa ccaggt                                                       16


<210>  34
<211>  736
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human AAVhu31 vp1

<400>  34

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro 
            20                  25                  30          


Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly 
145                 150                 155                 160 


Lys Ser Gly Ser Gln Pro Ala Lys Lys Lys Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 
            260                 265                 270         


Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 
        275                 280                 285             


Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 
    290                 295                 300                 


Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 
305                 310                 315                 320 


Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 
                325                 330                 335     


Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 
            340                 345                 350         


Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 
        355                 360                 365             


Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 
    370                 375                 380                 


Gly Gly Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 
385                 390                 395                 400 


Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 
                405                 410                 415     


Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 
            420                 425                 430         


Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 
        435                 440                 445             


Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser 
    450                 455                 460                 


Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro 
465                 470                 475                 480 


Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn 
                485                 490                 495     


Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn 
            500                 505                 510         


Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys 
        515                 520                 525             


Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly 
    530                 535                 540                 


Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile 
545                 550                 555                 560 


Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser 
                565                 570                 575     


Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln 
            580                 585                 590         


Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln 
        595                 600                 605             


Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 
    610                 615                 620                 


Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met 
625                 630                 635                 640 


Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 
                645                 650                 655     


Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr 
            660                 665                 670         


Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 
        675                 680                 685             


Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn 
    690                 695                 700                 


Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Ser Thr Glu Gly Val 
705                 710                 715                 720 


Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
                725                 730                 735     


<210>  35
<211>  736
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human AAVhu32 vp1

<400>  35

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro 
            20                  25                  30          


Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly 
145                 150                 155                 160 


Lys Ser Gly Ser Gln Pro Ala Lys Lys Lys Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 
            260                 265                 270         


Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 
        275                 280                 285             


Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 
    290                 295                 300                 


Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 
305                 310                 315                 320 


Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 
                325                 330                 335     


Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 
            340                 345                 350         


Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 
        355                 360                 365             


Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 
    370                 375                 380                 


Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 
385                 390                 395                 400 


Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 
                405                 410                 415     


Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 
            420                 425                 430         


Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 
        435                 440                 445             


Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser 
    450                 455                 460                 


Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro 
465                 470                 475                 480 


Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn 
                485                 490                 495     


Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn 
            500                 505                 510         


Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys 
        515                 520                 525             


Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly 
    530                 535                 540                 


Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile 
545                 550                 555                 560 


Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser 
                565                 570                 575     


Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln 
            580                 585                 590         


Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln 
        595                 600                 605             


Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 
    610                 615                 620                 


Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met 
625                 630                 635                 640 


Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 
                645                 650                 655     


Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr 
            660                 665                 670         


Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 
        675                 680                 685             


Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn 
    690                 695                 700                 


Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val 
705                 710                 715                 720 


Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
                725                 730                 735     


<210>  36
<211>  2211
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAV9 capsid vp1 coding sequence

<400>  36
atggctgccg atggttatct tccagattgg ctcgaggaca accttagtga aggaattcgc       60

gagtggtggg ctttgaaacc tggagcccct caacccaagg caaatcaaca acatcaagac      120

aacgctcgag gtcttgtgct tccgggttac aaataccttg gacccggcaa cggactcgac      180

aagggggagc cggtcaacgc agcagacgcg gcggccctcg agcacgacaa ggcctacgac      240

cagcagctca aggccggaga caacccgtac ctcaagtaca accacgccga cgccgagttc      300

caggagcggc tcaaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag      360

gccaaaaaga ggcttcttga acctcttggt ctggttgagg aagcggctaa gacggctcct      420

ggaaagaaga ggcctgtaga gcagtctcct caggaaccgg actcctccgc gggtattggc      480

aaatcgggtg cacagcccgc taaaaagaga ctcaatttcg gtcagactgg cgacacagag      540

tcagtcccag accctcaacc aatcggagaa cctcccgcag ccccctcagg tgtgggatct      600

cttacaatgg cttcaggtgg tggcgcacca gtggcagaca ataacgaagg tgccgatgga      660

gtgggtagtt cctcgggaaa ttggcattgc gattcccaat ggctggggga cagagtcatc      720

accaccagca cccgaacctg ggccctgccc acctacaaca atcacctcta caagcaaatc      780

tccaacagca catctggagg atcttcaaat gacaacgcct acttcggcta cagcaccccc      840

tgggggtatt ttgacttcaa cagattccac tgccacttct caccacgtga ctggcagcga      900

ctcatcaaca acaactgggg attccggcct aagcgactca acttcaagct cttcaacatt      960

caggtcaaag aggttacgga caacaatgga gtcaagacca tcgccaataa ccttaccagc     1020

acggtccagg tcttcacgga ctcagactat cagctcccgt acgtgctcgg gtcggctcac     1080

gagggctgcc tcccgccgtt cccagcggac gttttcatga ttcctcagta cgggtatctg     1140

acgcttaatg atggaagcca ggccgtgggt cgttcgtcct tttactgcct ggaatatttc     1200

ccgtcgcaaa tgctaagaac gggtaacaac ttccagttca gctacgagtt tgagaacgta     1260

cctttccata gcagctacgc tcacagccaa agcctggacc gactaatgaa tccactcatc     1320

gaccaatact tgtactatct ctcaaagact attaacggtt ctggacagaa tcaacaaacg     1380

ctaaaattca gtgtggccgg acccagcaac atggctgtcc agggaagaaa ctacatacct     1440

ggacccagct accgacaaca acgtgtctca accactgtga ctcaaaacaa caacagcgaa     1500

tttgcttggc ctggagcttc ttcttgggct ctcaatggac gtaatagctt gatgaatcct     1560

ggacctgcta tggccagcca caaagaagga gaggaccgtt tctttccttt gtctggatct     1620

ttaatttttg gcaaacaagg aactggaaga gacaacgtgg atgcggacaa agtcatgata     1680

accaacgaag aagaaattaa aactactaac ccggtagcaa cggagtccta tggacaagtg     1740

gccacaaacc accagagtgc ccaagcacag gcgcagaccg gctgggttca aaaccaagga     1800

atacttccgg gtatggtttg gcaggacaga gatgtgtacc tgcaaggacc catttgggcc     1860

aaaattcctc acacggacgg caactttcac ccttctccgc tgatgggagg gtttggaatg     1920

aagcacccgc ctcctcagat cctcatcaaa aacacacctg tacctgcgga tcctccaacg     1980

gccttcaaca aggacaagct gaactctttc atcacccagt attctactgg ccaagtcagc     2040

gtggagatcg agtgggagct gcagaaggaa aacagcaagc gctggaaccc ggagatccag     2100

tacacttcca actattacaa gtctaataat gttgaatttg ctgttaatac tgaaggtgta     2160

tatagtgaac cccgccccat tggcaccaga tacctgactc gtaatctgta a              2211


<210>  37
<211>  2211
<212>  DNA
<213>  Artificial sequence

<220>
<223>  AAVhu31 coding sequence

<400>  37
atggctgccg atggttatct tccagattgg ctcgaggaca accttagtga aggaattcgc       60

gagtggtggg ctttgaaacc tggagcccct caacccaagg caaatcaaca acatcaagac      120

aacgctcgag gtcttgtgct tccgggttac aaataccttg gacccggcaa cggactcgac      180

aagggggagc cggtcaacgc agcagacgcg gcggccctcg agcacgacaa ggcctacgac      240

cagcagctca aggccggaga caacccgtac ctcaagtaca accacgccga cgccgagttc      300

caggagcggc tcaaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag      360

gccaaaaaga ggcttcttga acctcttggt ctggttgagg aagcggctaa gacggctcct      420

ggaaagaaga ggcctgtaga gcagtctcct caggaaccgg actcctccgc gggtattggc      480

aaatcgggtg cacagcccgc taaaaagaga ctcaatttcg gtcagactgg cgacacagag      540

tcagtcccag accctcaacc aatcggagaa cctcccgcag ccccctcagg tgtgggatct      600

cttacaatgg cttcaggtgg tggcgcacca gtggcagaca ataacgaagg tgccgatgga      660

gtgggtagtt cctcgggaaa ttggcattgc gattcccaat ggctggggga cagagtcatc      720

accaccagca cccgaacctg ggccctgccc acctacaaca atcacctcta caagcaaatc      780

tccaacagca catctggagg atcttcaaat gacaacgcct acttcggcta cagcaccccc      840

tgggggtatt ttgacttcaa cagattccac tgccacttct caccacgtga ctggcagcga      900

ctcatcaaca acaactgggg attccggcct aagcgactca acttcaagct cttcaacatt      960

caggtcaaag aggttacgga caacaatgga gtcaagacca tcgccaataa ccttaccagc     1020

acggtccagg tcttcacgga ctcagactat cagctcccgt acgtgctcgg gtcggctcac     1080

gagggctgcc tcccgccgtt cccagcggac gttttcatga ttcctcagta cgggtatctg     1140

acgcttaatg atggaagcca ggccgtgggt cgttcgtcct tttactgcct ggaatatttc     1200

ccgtcgcaaa tgctaagaac gggtaacaac ttccagttca gctacgagtt tgagaacgta     1260

cctttccata gcagctacgc tcacagccaa agcctggacc gactaatgaa tccactcatc     1320

gaccaatact tgtactatct ctcaaagact attaacggtt ctggacagaa tcaacaaacg     1380

ctaaaattca gtgtggccgg acccagcaac atggctgtcc agggaagaaa ctacatacct     1440

ggacccagct accgacaaca acgtgtctca accactgtga ctcaaaacaa caacagcgaa     1500

tttgcttggc ctggagcttc ttcttgggct ctcaatggac gtaatagctt gatgaatcct     1560

ggacctgcta tggccagcca caaagaagga gaggaccgtt tctttccttt gtctggatct     1620

ttaatttttg gcaaacaagg aactggaaga gacaacgtgg atgcggacaa agtcatgata     1680

accaacgaag aagaaattaa aactactaac ccggtagcaa cggagtccta tggacaagtg     1740

gccacaaacc accagagtgc ccaagcacag gcgcagaccg gctgggttca aaaccaagga     1800

atacttccgg gtatggtttg gcaggacaga gatgtgtacc tgcaaggacc catttgggcc     1860

aaaattcctc acacggacgg caactttcac ccttctccgc tgatgggagg gtttggaatg     1920

aagcacccgc ctcctcagat cctcatcaaa aacacacctg tacctgcgga tcctccaacg     1980

gccttcaaca aggacaagct gaactctttc atcacccagt attctactgg ccaagtcagc     2040

gtggagatcg agtgggagct gcagaaggaa aacagcaagc gctggaaccc ggagatccag     2100

tacacttcca actattacaa gtctaataat gttgaatttg ctgttaatac tgaaggtgta     2160

tatagtgaac cccgccccat tggcaccaga tacctgactc gtaatctgta a              2211


<210>  38
<211>  2211
<212>  DNA
<213>  Artificial sequence

<220>
<223>  AAVhu32 VP1 coding sequence

<400>  38
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga       60

cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac      120

gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccggcaa cggactcgac      180

aagggggagc cggtcaacgc agcagacgcg gcggccctcg agcacgacaa ggcctacgac      240

cagcagctca aggccggaga caacccgtac ctcaagtaca accacgccga cgccgagttc      300

caggagcggc tcaaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag      360

gccaaaaaga ggcttcttga acctcttggt ctggttgagg aagcggctaa gacggctcct      420

ggaaagaaga ggcctgtaga gcagtctcct caggaaccgg actcctccgc gggtattggc      480

aaatcgggtt cacagcccgc taaaaagaaa ctcaatttcg gtcagactgg cgacacagag      540

tcagtccccg accctcaacc aatcggagaa cctcccgcag ccccctcagg tgtgggatct      600

cttacaatgg cttcaggtgg tggcgcacca gtggcagaca ataacgaagg tgccgatgga      660

gtgggtagtt cctcgggaaa ttggcattgc gattcccaat ggctggggga cagagtcatc      720

accaccagca cccgaacctg ggccctgccc acctacaaca atcacctcta caagcaaatc      780

tccaacagca catctggagg atcttcaaat gacaacgcct acttcggcta cagcaccccc      840

tgggggtatt ttgacttcaa cagattccac tgccacttct caccacgtga ctggcagcga      900

ctcatcaaca acaactgggg attccggcct aagcgactca acttcaagct cttcaacatt      960

caggtcaaag aggttacgga caacaatgga gtcaagacca tcgccaataa ccttaccagc     1020

acggtccagg tcttcacgga ctcagactat cagctcccgt acgtgctcgg gtcggctcac     1080

gagggctgcc tcccgccgtt cccagcggac gttttcatga ttcctcagta cgggtatctg     1140

acgcttaatg atgggagcca ggccgtgggt cgttcgtcct tttactgcct ggaatatttc     1200

ccgtcgcaaa tgctaagaac gggtaacaac ttccagttca gctacgagtt tgagaacgta     1260

cctttccata gcagctacgc tcacagccaa agcctggacc gactaatgaa tccactcatc     1320

gaccaatact tgtactatct ctcaaagact attaacggtt ctggacagaa tcaacaaacg     1380

ctaaaattca gcgtggccgg acccagcaac atggctgtcc agggaagaaa ctacatacct     1440

ggacccagct accgacaaca acgtgtctca accactgtga ctcaaaacaa caacagcgaa     1500

tttgcttggc ctggagcttc ttcttgggct ctcaatggac gtaatagctt gatgaatcct     1560

ggacctgcta tggccagcca caaagaagga gaggaccgtt tctttccttt gtctggatct     1620

ttaatttttg gcaaacaagg aactggaaga gacaacgtgg atgcggacaa agtcatgata     1680

accaacgaag aagaaattaa aactactaac ccggtagcaa cggagtccta tggacaagtg     1740

gccacaaacc accagagtgc ccaagcacag gcgcagaccg gctgggttca aaaccaagga     1800

atacttccgg gtatggtttg gcaggacaga gatgtgtacc tgcaaggacc catttgggcc     1860

aaaattcctc acacggacgg caactttcac ccttctccgc taatgggagg gtttggaatg     1920

aagcacccgc ctcctcagat cctcatcaaa aacacacctg tacctgcgga tcctccaacg     1980

gctttcaata aggacaagct gaactctttc atcacccagt attctactgg ccaagtcagc     2040

gtggagattg agtgggagct gcagaaggaa aacagcaagc gctggaaccc ggagatccag     2100

tacacttcca actattacaa gtctaataat gttgaatttg ctgttaatac tgaaggtgta     2160

tatagtgaac cccgccccat tggcaccaga tacctgactc gtaatctgta a              2211


<210>  39
<211>  736
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified hu68vp1


<220>
<221>  MISC_FEATURE
<222>  (23)..(23)
<223>  Xaa may be W (Trp, tryptophan), or oxidated W.

<220>
<221>  MISC_FEATURE
<222>  (35)..(35)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (57)..(57)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (66)..(66)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (94)..(94)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (97)..(97)
<223>  Xaa may be D (asp, aspartic acid), or isomerized D.

<220>
<221>  MISC_FEATURE
<222>  (107)..(107)
<223>  Xaa may be D (asp, aspartic acid), or isomerized D.

<220>
<221>  misc_feature
<222>  (113)..(113)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (149)..(149)
<223>  Xaa may be S (Ser, serine), or Phosphorilated S

<220>
<221>  MISC_FEATURE
<222>  (149)..(149)
<223>  Xaa may be S (Ser, serine), or Phosphorylated S

<220>
<221>  MISC_FEATURE
<222>  (247)..(247)
<223>  Xaa may be W (Trp, tryptophan), or oxidated W (e.g., kynurenine).

<220>
<221>  MISC_FEATURE
<222>  (253)..(253)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (259)..(259)
<223>  Xaa represents Q, or Q deamidated to glutamic acid 
       (alpha-glutamic acid), gamma-glutamic acid (Glu), or a blend of 
       alpha- and gamma-glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (270)..(270)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (297)..(297)
<223>  Xaa represents D (Asp, aspartic acid) or amindated D to N (Asn, 
       asparagine)

<220>
<221>  MISC_FEATURE
<222>  (304)..(304)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (306)..(306)
<223>  Xaa may be W (Trp, tryptophan), or oxidated W (e.g., kynurenine).

<220>
<221>  MISC_FEATURE
<222>  (314)..(314)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (319)..(319)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (329)..(329)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (332)..(332)
<223>  Xaa may be K (lys, lysine), or acetylated K

<220>
<221>  MISC_FEATURE
<222>  (336)..(336)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (384)..(384)
<223>  Xaa may be D (asp, aspartic acid), or isomerized D.

<220>
<221>  MISC_FEATURE
<222>  (404)..(404)
<223>  Xaa may be M (Met, Methionine), or oxidated M.

<220>
<221>  MISC_FEATURE
<222>  (409)..(409)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (436)..(436)
<223>  Xaa may be M (Met, Methionine), or oxidated M.

<220>
<221>  MISC_FEATURE
<222>  (452)..(452)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (477)..(477)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (499)..(499)
<223>  Xaa may be S (Ser, serine), or Phosphorylated S

<220>
<221>  MISC_FEATURE
<222>  (512)..(512)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (515)..(515)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (518)..(518)
<223>  Xaa may be M (Met, Methionine), or oxidated M.

<220>
<221>  MISC_FEATURE
<222>  (524)..(524)
<223>  Xaa may be M (Met, Methionine), or oxidated M.

<220>
<221>  MISC_FEATURE
<222>  (559)..(559)
<223>  Xaa may be M (Met, Methionine), or oxidated M.

<220>
<221>  MISC_FEATURE
<222>  (569)..(569)
<223>  Xaa may be T (Thr, threonine), or Phosphorylated T

<220>
<221>  MISC_FEATURE
<222>  (586)..(586)
<223>  Xaa may be S (Ser, serine), or Phosphorylated S

<220>
<221>  MISC_FEATURE
<222>  (599)..(599)
<223>  Xaa represents Q, or Q deamidated to glutamic acid 
       (alpha-glutamic acid), gamma-glutamic acid (Glu), or a blend of 
       alpha- and gamma-glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (605)..(605)
<223>  Xaa may be M (Met, Methionine), or oxidated M.

<220>
<221>  MISC_FEATURE
<222>  (619)..(619)
<223>  Xaa may be W (Trp, tryptophan), or oxidated W (e.g., kynurenine).

<220>
<221>  MISC_FEATURE
<222>  (628)..(628)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (640)..(640)
<223>  Xaa may be M (Met, Methionine), or oxidated M.

<220>
<221>  MISC_FEATURE
<222>  (651)..(651)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (663)..(663)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (666)..(666)
<223>  Xaa may be K (lys, lysine), or acetylated K

<220>
<221>  MISC_FEATURE
<222>  (689)..(689)
<223>  Xaa may be K (lys, lysine), or acetylated K

<220>
<221>  MISC_FEATURE
<222>  (693)..(693)
<223>  Xaa may be K (lys, lysine), or acetylated K

<220>
<221>  MISC_FEATURE
<222>  (695)..(695)
<223>  Xaa may be W (Trp, tryptophan), or oxidated W.

<220>
<221>  MISC_FEATURE
<222>  (709)..(709)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<220>
<221>  MISC_FEATURE
<222>  (735)..(735)
<223>  Xaa may be Asn, or deamidated to Asp, isoAsp, or Asp/isoAsp

<400>  39

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Xaa Ala Leu Lys Pro Gly Ala Pro Gln Pro 
            20                  25                  30          


Lys Ala Xaa Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Gly Xaa Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Xaa Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Xaa His Ala 
                85                  90                  95      


Xaa Ala Glu Phe Gln Glu Arg Leu Lys Glu Xaa Thr Ser Phe Gly Gly 
            100                 105                 110         


Xaa Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Gln Xaa Pro Gln Glu Pro Asp Ser Ser Val Gly Ile Gly 
145                 150                 155                 160 


Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Xaa Ala Leu Pro Thr Tyr Xaa Asn His Leu 
                245                 250                 255     


Tyr Lys Xaa Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Xaa Asp Asn 
            260                 265                 270         


Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 
        275                 280                 285             


Phe His Cys His Phe Ser Pro Arg Xaa Trp Gln Arg Leu Ile Asn Xaa 
    290                 295                 300                 


Asn Xaa Gly Phe Arg Pro Lys Arg Leu Xaa Phe Lys Leu Phe Xaa Ile 
305                 310                 315                 320 


Gln Val Lys Glu Val Thr Asp Asn Xaa Gly Val Xaa Thr Ile Ala Xaa 
                325                 330                 335     


Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 
            340                 345                 350         


Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 
        355                 360                 365             


Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Xaa 
    370                 375                 380                 


Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 
385                 390                 395                 400 


Pro Ser Gln Xaa Leu Arg Thr Gly Xaa Asn Phe Gln Phe Ser Tyr Glu 
                405                 410                 415     


Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 
            420                 425                 430         


Asp Arg Leu Xaa Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 
        435                 440                 445             


Lys Thr Ile Xaa Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser 
    450                 455                 460                 


Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Xaa Tyr Ile Pro 
465                 470                 475                 480 


Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn 
                485                 490                 495     


Asn Asn Xaa Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Xaa 
            500                 505                 510         


Gly Arg Xaa Ser Leu Xaa Asn Pro Gly Pro Ala Xaa Ala Ser His Lys 
        515                 520                 525             


Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly 
    530                 535                 540                 


Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Xaa Ile 
545                 550                 555                 560 


Thr Asn Glu Glu Glu Ile Lys Thr Xaa Asn Pro Val Ala Thr Glu Ser 
                565                 570                 575     


Tyr Gly Gln Val Ala Thr Asn His Gln Xaa Ala Gln Ala Gln Ala Gln 
            580                 585                 590         


Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Xaa Val Trp Gln 
        595                 600                 605             


Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Xaa Ala Lys Ile Pro His 
    610                 615                 620                 


Thr Asp Gly Xaa Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Xaa 
625                 630                 635                 640 


Lys His Pro Pro Pro Gln Ile Leu Ile Lys Xaa Thr Pro Val Pro Ala 
                645                 650                 655     


Asp Pro Pro Thr Ala Phe Xaa Lys Asp Xaa Leu Asn Ser Phe Ile Thr 
            660                 665                 670         


Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 
        675                 680                 685             


Xaa Glu Asn Ser Xaa Arg Xaa Asn Pro Glu Ile Gln Tyr Thr Ser Asn 
    690                 695                 700                 


Tyr Tyr Lys Ser Xaa Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val 
705                 710                 715                 720 


Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Xaa Leu 
                725                 730                 735     


