                         LISTAGEM DE SEQUNCIAS

<110>  Fundao Butantan
 
<120>  PROTENA RECOMBINANTE E SEUS FRAGMENTOS, PROCESSO DE 
       OBTENO DA PROTENA RECOMBINANTE, GENE SINTTICO E USO 
       DA ESCULPTINA OU PROTENA RECOMBINANTE PARA PREPARAR UM 
       MEDICAMENTO OU COMPOSIO FARMACUTICA PARA PROFILAXIA 
       E/OU TRATAMENTO DE DOENAS TROMBOEMBLICAS OU COMO 
       INIBIDOR DIRETO E ESPECFICO DA TROMBINA 

<130>  BR 10 2017 005783 6

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  157
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Protena recombinante escuptina

<400>  1

Gly Lys Pro Gln Gly His Pro His Asp Ala Leu Glu Ala Arg Ser Asp 
1               5                   10                  15      


Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly Gly His Gly Gly 
            20                  25                  30          


Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala Leu Glu Ala Arg 
        35                  40                  45              


Ser Asp Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly Gly Leu 
    50                  55                  60                  


Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala Leu Glu 
65                  70                  75                  80  


Ala Arg Ser Asp Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly 
                85                  90                  95      


Gly His Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala 
            100                 105                 110         


Leu Glu Ala Arg Ser Asp Ala Val His Thr Ala Val Pro Lys Met Pro 
        115                 120                 125             


Lys Gly Gly His Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu 
    130                 135                 140                 


Arg Ala Leu His Ala Leu Glu His His His His His His 
145                 150                 155         


<210>  2
<211>  24
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Fragmentos de esculptina gerados por trombina

<400>  2

Gly Lys Pro Gln Gly His Pro His Asp Ala Leu Glu Ala Arg Ser Asp 
1               5                   10                  15      


Ala Val His Thr Ala Val Pro Lys 
            20                  


<210>  3
<211>  58
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Fragmentos de esculptina gerados por trombina

<400>  3

Gly Lys Pro Gln Gly His Pro His Asp Ala Leu Glu Ala Arg Ser Asp 
1               5                   10                  15      


Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly Gly His Gly Gly 
            20                  25                  30          


Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala Leu Glu Ala Arg 
        35                  40                  45              


Ser Asp Ala Val His Thr Ala Val Pro Lys 
    50                  55              


<210>  4
<211>  34
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Fragmentos de esculptina gerados por trombina

<400>  4

Met Pro Lys Gly Gly His Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr 
1               5                   10                  15      


Asp Glu Arg Ala Leu Glu Ala Arg Ser Asp Ala Val His Thr Ala Val 
            20                  25                  30          


Pro Lys 
        


<210>  5
<211>  31
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Fragmentos de esculptina gerados por trombina

<400>  5

Met Pro Lys Gly Gly His Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr 
1               5                   10                  15      


Asp Glu Arg Ala Leu His Ala Leu Glu His His His His His His 
            20                  25                  30      


<210>  6
<211>  65
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Fragmentos de esculptina gerados por trombina

<400>  6

Met Pro Lys Gly Gly His Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr 
1               5                   10                  15      


Asp Glu Arg Ala Leu Glu Ala Arg Ser Asp Ala Val His Thr Ala Val 
            20                  25                  30          


Pro Lys Met Pro Lys Gly Gly His Gly Gly Phe Glu Pro Ile Pro Ile 
        35                  40                  45              


Asp Tyr Asp Glu Arg Ala Leu His Ala Leu Glu His His His His His 
    50                  55                  60                  


His 
65  


<210>  7
<211>  68
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Fragmentos de esculptina gerados por trombina

<400>  7

Met Pro Lys Gly Gly His Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr 
1               5                   10                  15      


Asp Glu Arg Ala Leu Glu Ala Arg Ser Asp Ala Val His Thr Ala Val 
            20                  25                  30          


Pro Lys Met Pro Lys Gly Gly Leu Gly Gly Phe Glu Pro Ile Pro Ile 
        35                  40                  45              


Asp Tyr Asp Glu Arg Ala Leu Glu Ala Arg Ser Asp Ala Val His Thr 
    50                  55                  60                  


Ala Val Pro Lys 
65              


<210>  8
<211>  99
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Fragmentos de esculptina gerados por trombina

<400>  8

Met Pro Lys Gly Gly Leu Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr 
1               5                   10                  15      


Asp Glu Arg Ala Leu Glu Ala Arg Ser Asp Ala Val His Thr Ala Val 
            20                  25                  30          


Pro Lys Met Pro Lys Gly Gly His Gly Gly Phe Glu Pro Ile Pro Ile 
        35                  40                  45              


Asp Tyr Asp Glu Arg Ala Leu Glu Ala Arg Ser Asp Ala Val His Thr 
    50                  55                  60                  


Ala Val Pro Lys Met Pro Lys Gly Gly His Gly Gly Phe Glu Pro Ile 
65                  70                  75                  80  


Pro Ile Asp Tyr Asp Glu Arg Ala Leu His Ala Leu Glu His His His 
                85                  90                  95      


His His His 
            


<210>  9
<211>  133
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Fragmentos de esculptina gerados por trombina

<400>  9

Met Pro Lys Gly Gly His Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr 
1               5                   10                  15      


Asp Glu Arg Ala Leu Glu Ala Arg Ser Asp Ala Val His Thr Ala Val 
            20                  25                  30          


Pro Lys Met Pro Lys Gly Gly Leu Gly Gly Phe Glu Pro Ile Pro Ile 
        35                  40                  45              


Asp Tyr Asp Glu Arg Ala Leu Glu Ala Arg Ser Asp Ala Val His Thr 
    50                  55                  60                  


Ala Val Pro Lys Met Pro Lys Gly Gly His Gly Gly Phe Glu Pro Ile 
65                  70                  75                  80  


Pro Ile Asp Tyr Asp Glu Arg Ala Leu Glu Ala Arg Ser Asp Ala Val 
                85                  90                  95      


His Thr Ala Val Pro Lys Met Pro Lys Gly Gly His Gly Gly Phe Glu 
            100                 105                 110         


Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala Leu His Ala Leu Glu His 
        115                 120                 125             


His His His His His 
    130             


<210>  10
<211>  48
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Fragmentos de esculptina gerados pelo fator Xa

<400>  10

Gly Lys Pro Gln Gly His Pro His Asp Ala Leu Glu Ala Arg Ser Asp 
1               5                   10                  15      


Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly Gly His Gly Gly 
            20                  25                  30          


Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala Leu Glu Ala Arg 
        35                  40                  45              


<210>  11
<211>  34
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Fragmentos de esculptina gerados pelo fator Xa

<400>  11

Ser Asp Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly Gly His 
1               5                   10                  15      


Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala Leu Glu 
            20                  25                  30          


Ala Arg 
        


<210>  12
<211>  41
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Fragmentos de esculptina gerados pelo fator Xa

<400>  12

Ser Asp Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly Gly His 
1               5                   10                  15      


Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala Leu His 
            20                  25                  30          


Ala Leu Glu His His His His His His 
        35                  40      


<210>  13
<211>  75
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Fragmentos de esculptina gerados pelo fator Xa

<400>  13

Ser Asp Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly Gly His 
1               5                   10                  15      


Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala Leu Glu 
            20                  25                  30          


Ala Arg Ser Asp Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly 
        35                  40                  45              


Gly His Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala 
    50                  55                  60                  


Leu His Ala Leu Glu His His His His His His 
65                  70                  75  


<210>  14
<211>  63
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Fragmentos de esculptina gerdados pelo fator Xa

<400>  14

Ser Asp Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly Gly His 
1               5                   10                  15      


Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala Leu Glu 
            20                  25                  30          


Ala Arg Ser Asp Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly 
        35                  40                  45              


Gly His Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg 
    50                  55                  60              


<210>  15
<211>  68
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Fragmentos de esculptina gerados pelo fator Xa

<400>  15

Ser Asp Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly Gly His 
1               5                   10                  15      


Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala Leu Glu 
            20                  25                  30          


Ala Arg Ser Asp Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly 
        35                  40                  45              


Gly His Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala 
    50                  55                  60                  


Leu Glu Ala Arg 
65              


<210>  16
<211>  116
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Fragmentos de esculptina gerados pelo fator Xa

<400>  16

Gly Lys Pro Gln Gly His Pro His Asp Ala Leu Glu Ala Arg Ser Asp 
1               5                   10                  15      


Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly Gly His Gly Gly 
            20                  25                  30          


Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala Leu Glu Ala Arg 
        35                  40                  45              


Ser Asp Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly Gly Leu 
    50                  55                  60                  


Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala Leu Glu 
65                  70                  75                  80  


Ala Arg Ser Asp Ala Val His Thr Ala Val Pro Lys Met Pro Lys Gly 
                85                  90                  95      


Gly His Gly Gly Phe Glu Pro Ile Pro Ile Asp Tyr Asp Glu Arg Ala 
            100                 105                 110         


Leu Glu Ala Arg 
        115     


<210>  17
<211>  477
<212>  DNA
<213>  Sequncia artificial

<220>
<223>  gene sinttico codificador da esculptina

<400>  17
atgggtaagc ctcaagggca tccacacgac gcactggagg cccgtagcga cgcagtgcat       60

accgctgtcc ctaagatgcc gaagggcggc cacggtgggt ttgaaccgat cccgattgat      120

tatgacgaac gcgcactgga agctcgcagt gacgcggtac atactgctgt gcctaaaatg      180

ccaaaagggg gcctgggggg tttcgagccg atcccgattg attatgacga gcgcgcactg      240

gaagctcgta gcgacgcagt acatactgct gtaccaaaaa tgcctaaagg gggtcacggg      300

gggtttgaac cgattccgat cgattacgac gaacgtgcgc tggaagcgcg ttctgatgcc      360

gtccatacgg cggtgccaaa aatgcctaaa ggcggtcatg gtggcttcga acctattcca      420

atcgattacg acgaacgtgc cctgcacgca ctcgagcacc accaccacca ccactga         477


