                         SEQUENCE LISTING

<110>  BIOGEMMA
 
<120>  METHOD FOR DETERMINING THE LEVEL OF ZYGOSITY OF A SEED

<130>  BRV 123-WO

<150>  EP17305341.4
<151>  2017-03-24

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1249
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMWG promoter from wheat from GenBank AJ301618

<400>  1
gcatgcaaat atgcaacata atttcctttt tacttggcta attatatttg ataaatattt       60

cacagatata caataatcaa acacaataaa tcatatgtgt ttttagtttt agttctcata      120

tccaaatata caatagctaa ccaaatctca tcgggaagtt agccatgccg aggtaggttg      180

ttgccggaat gtttttagtt ttagttctca tacaaccaaa tctcattcaa atatataaaa      240

cattccggca acaacttgtg gcgtacatct agttacaagg gaatattagt gatggcgtga      300

gcaagcgata aggccaagga gagaagaagt gcatcgtcta cggaggccag ggaaagacaa      360

tggacatgca gagaggcagg ggcggggaag aaacacttgg agatcataga agaagataag      420

aggttaaaca taggaggagg atataatgga caattaaatc tgcgttagtt gaactcattt      480

gggaagtaaa caaattttct attctgtgta aaccaaacta tttgacgcgg attttctctg      540

aagatcctat attaatttta gacatggttt ggctagttca tttgtcgtga aaaggtgttt      600

ccataagtcc aaaattctac caactttttt gtatggcacg tcatagcata gatagatgtt      660

gtgagtcact ggatagatat tgtgagtcat agcatggatt cgtgttgctg gaaatccaac      720

tacatgacaa gcaacaaaac ctgaaatggg ctttaggagt taacaattta cttgttccat      780

gcaggctacc ttccactact cgacatgctt agaagctttg agtggccgta gatttgcaaa      840

agcaatggct aacagacaca tattctgcca aaccccaaga aggataatca cttttcttag      900

ataaaaaaga acagaccaat atacaaacat ccacacttct gcaaacaata catcagaact      960

aggattacgc cgattacgtg gctttagcag actgtccaaa aatctgtttt gcaaagctcc     1020

aattgctcct tgcttatcca gcttcttttg tgttggcaaa ctgcgctttt ccaaccgatt     1080

ttgttcttct cgcgctttct tcttaggcta aacaaacctc accgtgcacg cagccatggt     1140

cctgaacctt cacctcgtcc ctataaaagc ctagccaacc ttcacaatct tatcatcacc     1200

cacaacaccg agcaccacaa actagagatc aattcactga tagtccacc                 1249


<210>  2
<211>  417
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  promoter HMWG of wheat (Triticum aestivum), bases 814 to 1220 of 
       SEQ ID NO: 1

<400>  2
agctttgagt ggccgtagat ttgcaaaagc aatggctaac agacacatat tctgccaaac       60

cccaagaagg ataatcactt ttcttagata aaaaagaaca gaccaatata caaacatcca      120

cacttctgca aacaatacat cagaactagg attacgccga ttacgtggct ttagcagact      180

gtccaaaaat ctgttttgca aagctccaat tgctccttgc ttatccagct tcttttgtgt      240

tggcaaactg cgcttttcca accgattttg ttcttctcgc gctttcttct tagcctaaac      300

aaacctcacc gtgcacgcag ccatggtcct gaaccttcac ctcgtcccta taaaagccta      360

gccaaccttc acaatcttat catcacccac aacaccgagc accacaaact agagatc         417


<210>  3
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZsGreen_ZmMod Fluorescent protein coding sequence

<400>  3
atggctcagt caaagcacgg gctcacaaag gagatgacta tgaagtacag gatggagggc       60

tgcgtcgatg ggcacaagtt cgttatcacc ggcgagggga tcggctaccc gttcaagggc      120

aagcaggcga ttaacctgtg cgtggtcgag ggcgggccac tgcccttcgc tgaggatatc      180

ctctccgccg cgttcatgta cggcaacagg gttttcaccg agtacccgca ggacattgtc      240

gattacttca agaattcctg cccagctggg tacacgtggg acaggagctt cctcttcgag      300

gatggcgctg tgtgcatctg caacgccgac attaccgttt cggtggagga gaattgcatg      360

taccacgagt ctaagttcta cggggtgaac ttcccagctg acggccccgt catgaagaag      420

atgacggata attgggagcc atcatgcgag aagatcattc cagtgcctaa gcaggggatc      480

ctgaagggcg acgtctccat gtacctcctg ctcaaggatg gcggcaggct caggtgccag      540

ttcgacacag tctacaaggc caagagcgtt ccacgcaaga tgcctgactg gcacttcatc      600

cagcataagc tgactcggga ggaccgctcg gatgcgaaga accagaagtg gcacctgact      660

gagcacgcta tcgcctcggg gtccgccctc ccttga                                696


<210>  4
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fluorescent protein coding sequence derived from Aequorea 
       victoria

<400>  4
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccttcaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcagc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac      480

ggcatcaagg ccaacttcaa gatccgccac aacgtcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa      720


<210>  5
<211>  1363
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct proTaHMWG - ZsGreen_ZmMod - terAtNOS

<400>  5
agctttgagt ggccgtagat ttgcaaaagc aatggctaac agacacatat tctgccaaac       60

cccaagaagg ataatcactt ttcttagata aaaaagaaca gaccaatata caaacatcca      120

cacttctgca aacaatacat cagaactagg attacgccga ttacgtggct ttagcagact      180

gtccaaaaat ctgttttgca aagctccaat tgctccttgc ttatccagct tcttttgtgt      240

tggcaaactg cgcttttcca accgattttg ttcttctcgc gctttcttct tagcctaaac      300

aaacctcacc gtgcacgcag ccatggtcct gaaccttcac ctcgtcccta taaaagccta      360

gccaaccttc acaatcttat catcacccac aacaccgagc accacaaact agagatcatg      420

gctcagtcaa agcacgggct cacaaaggag atgactatga agtacaggat ggagggctgc      480

gtcgatgggc acaagttcgt tatcaccggc gaggggatcg gctacccgtt caagggcaag      540

caggcgatta acctgtgcgt ggtcgagggc gggccactgc ccttcgctga ggatatcctc      600

tccgccgcgt tcatgtacgg caacagggtt ttcaccgagt acccgcagga cattgtcgat      660

tacttcaaga attcctgccc agctgggtac acgtgggaca ggagcttcct cttcgaggat      720

ggcgctgtgt gcatctgcaa cgccgacatt accgtttcgg tggaggagaa ttgcatgtac      780

cacgagtcta agttctacgg ggtgaacttc ccagctgacg gccccgtcat gaagaagatg      840

acggataatt gggagccatc atgcgagaag atcattccag tgcctaagca ggggatcctg      900

aagggcgacg tctccatgta cctcctgctc aaggatggcg gcaggctcag gtgccagttc      960

gacacagtct acaaggccaa gagcgttcca cgcaagatgc ctgactggca cttcatccag     1020

cataagctga ctcgggagga ccgctcggat gcgaagaacc agaagtggca cctgactgag     1080

cacgctatcg cctcggggtc cgccctccct tgagatcgtt caaacatttg gcaataaagt     1140

ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat     1200

tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt     1260

atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca     1320

aactaggata aattatcgcg cgcggtgtca tctatgttac tag                       1363


<210>  6
<211>  250
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nopaline synthase (NOS) terminator from Arabidopsis thaliana

<400>  6
gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg       60

atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc      120

atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac      180

gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct      240

atgttactag                                                             250


