                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
       Zhao, Yangbing
       Liu, Xiaojun
       Pan, Wei
 
<120>  USE OF GENE EDITING TO GENERATE UNIVERSAL TCR RE-DIRECTED T CELLS
        FOR ADOPTIVE IMMUNOTHERAPY

<130>  046483-7159WO1(01563)

<150>  62/485,166
<151>  2017-04-13

<160>  140   

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  1
caaacacagc gacctcgggt                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  2
ggctcaaaca cagcgacctc                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  3
tcaaacacag cgacctcggg                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  4
tggctcaaac acagcgacct                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  5
tctccgagag cccgtagaac                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  6
ggctctcgga gaatgacgag                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  7
tgacagcgga agtggttgcg                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  8
agtccagttc tacgggctct                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  9
cgctgtcaag tccagttcta                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  10
agctcagctc cacgtggtcg                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  11
actggacttg acagcggaag                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  12
ttgacagcgg aagtggttgc                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  13
gacagcggaa gtggttgcgg                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  14
tgacgagtgg acccaggata                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  15
cgtagaactg gacttgacag                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  16
atgacgagtg gacccaggat                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  17
cttgacagcg gaagtggttg                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  18
gctgtcaagt ccagttctac                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  19
aggcctcggc gctgacgatc                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  20
ggcctcggcg ctgacgatct                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  21
cacccagatc gtcagcgccg                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  22
gacgatctgg gtgacgggtt                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  23
gatcgtcagc gccgaggcct                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  24
agatcgtcag cgccgaggcc                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  25
ctccaggtag ccaccttcta                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  26
gctgaactgg tcgcagttga                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  27
tcaactgcga ccagttcagc                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  28
gggagtcctg gaagacatac                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  29
ctctcaccga tcacttcatc                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  30
aggtctgccg gaagctcctc                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  31
cggttttctc caggcgcatc                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  32
ggctcctggt tgaacagcgc                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  33
caaactggat gggtccctca                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  34
caggcagctc aacgaggaac                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  35
ccaacatctc cagaccggcc                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  36
ctctccagct gccgggcatt                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  37
acgtatggtg ccgagcccgc                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  38
gagcggtaga actgctccac                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  39
gagtctgcac aagctttccc                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  40
ttctttaggt cccgaacagc                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  41
tctttaggtc ccgaacagca                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  42
caaactggtg cggatcctca                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  43
gagaacaaga tcggggacga                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  44
gggtgcctac aaactcgccg                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  45
aataactgca tctgcgacgt                                                   20


<210>  46
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  46
caataactgc atctgcgacg                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  47
tacaacaagt tcacggctgc                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  48
tccgtgaatc ctgttgttgc                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  49
tgtagcaccg cccagacgac                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  50
cgtctgggcg gtgctacaac                                                   20


<210>  51
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  51
gtctgggcgg tgctacaact                                                   20


<210>  52
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  52
aggcgccctg gccagtcgtc                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  53
caccgcccag acgactggcc                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  54
atgtggaagt cacgcccgtt                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  55
catgtggaag tcacgcccgt                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  56
cacgaagctc tccgatgtgt                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  57
cggagagctt cgtgctaaac                                                   20


<210>  58
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  58
cctgctcgtg gtgaccgaag                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  59
ccccttcggt caccacgagc                                                   20


<210>  60
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  60
aggcggccag cttgtccgtc                                                   20


<210>  61
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  61
gccctgctcg tggtgaccga                                                   20


<210>  62
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  62
cccttcggtc accacgagca                                                   20


<210>  63
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  63
ccctgctcgt ggtgaccgaa                                                   20


<210>  64
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  64
gcgtgacttc cacatgagcg                                                   20


<210>  65
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  65
aggtgccgct gtcattgcgc                                                   20


<210>  66
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  66
acttccacat gagcgtggtc                                                   20


<210>  67
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  67
ggtgccgctg tcattgcgcc                                                   20


<210>  68
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  68
accctggtgg ttggtgtcgt                                                   20


<210>  69
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  69
agggtttgga actggccggc                                                   20


<210>  70
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  70
attgtctttc ctagcggaat                                                   20


<210>  71
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  71
tcagtggctg ggcactccga                                                   20


<210>  72
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  72
cattgtcttt cctagcggaa                                                   20


<210>  73
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  73
gagtagcgcg agcacagcta                                                   20


<210>  74
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  74
cgcgagcaca gctaaggcca                                                   20


<210>  75
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  75
ctcgcgctac tctctctttc                                                   20


<210>  76
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  76
aagtcaactt caatgtcgga                                                   20


<210>  77
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  77
cgtgagtaaa cctgaatctt                                                   20


<210>  78
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  78
acccagacac atagcaattc                                                   20


<210>  79
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  79
ttcctgaatt gctatgtgtc                                                   20


<210>  80
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  80
cagtaagtca acttcaatgt                                                   20


<210>  81
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  81
tcctgaattg ctatgtgtct                                                   20


<210>  82
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  82
ggcatactca tctttttcag                                                   20


<210>  83
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  83
acagcccaag atagttaagt                                                   20


<210>  84
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  84
cacagcccaa gatagttaag                                                   20


<210>  85
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  85
gagaatcaaa atcggtgaat                                                   20


<210>  86
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  86
tgtgctagac atgaggtcta                                                   20


<210>  87
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  87
aaagtcagat ttgttgctcc                                                   20


<210>  88
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  88
agagtctctc agctggtaca                                                   20


<210>  89
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  89
agctggtaca cggcagggtc                                                   20


<210>  90
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  90
acaaaactgt gctagacatg                                                   20


<210>  91
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  91
ctcgaccagc ttgacatcac                                                   20


<210>  92
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  92
aagttcctgt gatgtcaagc                                                   20


<210>  93
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  93
ttcggaaccc aatcactgac                                                   20


<210>  94
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  94
ttaatctgct catgacgctg                                                   20


<210>  95
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  95
gattaaaccc ggccactttc                                                   20


<210>  96
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  96
cgtcatgagc agattaaacc                                                   20


<210>  97
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA

<400>  97
taaacccggc cactttcagg                                                   20


<210>  98
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  NY-ESO epitope

<400>  98

Ser Leu Leu Met Trp Ile Thr Gln Cys 
1               5                   


<210>  99
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker


<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  can be repeated n times, where n is an integer of at least 1

<400>  99

Gly Ser Gly Gly Ser 
1               5   


<210>  100
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  can be repeated n times, where n is an integer of at least 1

<400>  100

Gly Gly Gly Ser 
1               


<210>  101
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker


<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  can be repeated n times, where n is an integer of at least 1

<400>  101

Gly Gly Gly Gly Ser 
1               5   


<210>  102
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker

<400>  102

Gly Gly Ser Gly 
1               


<210>  103
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker

<400>  103

Gly Gly Ser Gly Gly 
1               5   


<210>  104
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker

<400>  104

Gly Ser Gly Ser Gly 
1               5   


<210>  105
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker

<400>  105

Gly Ser Gly Gly Gly 
1               5   


<210>  106
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker

<400>  106

Gly Gly Gly Ser Gly 
1               5   


<210>  107
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker

<400>  107

Gly Ser Ser Ser Gly 
1               5   


<210>  108
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker

<400>  108

Gly Gly Gly Gly Ser 
1               5   


<210>  109
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker

<400>  109

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  110
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker

<400>  110

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  111
<211>  45
<212>  DNA
<213>  Artificial sequence

<220>
<223>  linker

<400>  111
ggtggcggtg gctcgggcgg tggtgggtcg ggtggcggcg gatct                       45


<210>  112
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hinge

<400>  112

Asp Lys Thr His Thr 
1               5   


<210>  113
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hinge

<400>  113

Cys Pro Pro Cys 
1               


<210>  114
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hinge

<400>  114

Cys Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg 
1               5                   10                  15  


<210>  115
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hinge

<400>  115

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr 
1               5                   10          


<210>  116
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hinge

<400>  116

Lys Ser Cys Asp Lys Thr His Thr Cys Pro 
1               5                   10  


<210>  117
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hinge

<400>  117

Lys Cys Cys Val Asp Cys Pro 
1               5           


<210>  118
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hinge

<400>  118

Lys Tyr Gly Pro Pro Cys Pro 
1               5           


<210>  119
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hinge

<400>  119

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  120
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hinge

<400>  120

Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro 
1               5                   10          


<210>  121
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hinge

<400>  121

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro Arg Cys 
1               5                   10                  15      


Pro 
    


<210>  122
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hinge

<400>  122

Ser Pro Asn Met Val Pro His Ala His His Ala Gln 
1               5                   10          


<210>  123
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hinge

<400>  123

Glu Pro Lys Ser Cys Asp Lys Thr Tyr Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  124
<211>  238
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TGFBR-IL12RB1 receptor

<400>  124

Met Glu Ala Ala Val Ala Ala Pro Arg Pro Arg Leu Leu Leu Leu Val 
1               5                   10                  15      


Leu Ala Ala Ala Ala Ala Ala Ala Ala Ala Leu Leu Pro Gly Ala Thr 
            20                  25                  30          


Ala Leu Gln Cys Phe Cys His Leu Cys Thr Lys Asp Asn Phe Thr Cys 
        35                  40                  45              


Val Thr Asp Gly Leu Cys Phe Val Ser Val Thr Glu Thr Thr Asp Lys 
    50                  55                  60                  


Val Ile His Asn Ser Met Cys Ile Ala Glu Ile Asp Leu Ile Pro Arg 
65                  70                  75                  80  


Asp Arg Pro Phe Val Cys Ala Pro Ser Ser Lys Thr Gly Ser Val Thr 
                85                  90                  95      


Thr Thr Tyr Cys Cys Asn Gln Asp His Cys Asn Lys Ile Glu Leu Pro 
            100                 105                 110         


Thr Thr Val Lys Ser Ser Pro Gly Leu Gly Pro Val Glu Leu Ala Ala 
        115                 120                 125             


Val Ile Ala Gly Pro Val Cys Phe Val Cys Ile Ser Leu Met Leu Met 
    130                 135                 140                 


Val Tyr Ile Arg Ala Ala Arg His Leu Cys Pro Pro Leu Pro Thr Pro 
145                 150                 155                 160 


Cys Ala Ser Ser Ala Ile Glu Phe Pro Gly Gly Lys Glu Thr Trp Gln 
                165                 170                 175     


Trp Ile Asn Pro Val Asp Phe Gln Glu Glu Ala Ser Leu Gln Glu Ala 
            180                 185                 190         


Leu Val Val Glu Met Ser Trp Asp Lys Gly Glu Arg Thr Glu Pro Leu 
        195                 200                 205             


Glu Lys Thr Glu Leu Pro Glu Gly Ala Pro Glu Leu Ala Leu Asp Thr 
    210                 215                 220                 


Glu Leu Ser Leu Glu Asp Gly Asp Arg Cys Lys Ala Lys Met 
225                 230                 235             


<210>  125
<211>  714
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TGFBR-IL12RB1 receptor

<400>  125
atggaggcgg cggtcgctgc tccgcgtccc cggctgctcc tcctcgtgct ggcggcggcg       60

gcggcggcgg cggcggcgct gctcccgggg gcgacggcgt tacagtgttt ctgccacctc      120

tgtacaaaag acaattttac ttgtgtgaca gatgggctct gctttgtctc tgtcacagag      180

accacagaca aagttataca caacagcatg tgtatagctg aaattgactt aattcctcga      240

gataggccgt ttgtatgtgc accctcttca aaaactgggt ctgtgactac aacatattgc      300

tgcaatcagg accattgcaa taaaatagaa cttccaacta ctgtaaagtc atcacctggc      360

cttggtcctg tggaactggc agctgtcatt gctggaccag tgtgcttcgt ctgcatctca      420

ctcatgttga tggtctatat cagggccgca cggcacctgt gcccgccgct gcccacaccc      480

tgtgccagct ccgccattga gttccctgga gggaaggaga cttggcagtg gatcaaccca      540

gtggacttcc aggaagaggc atccctgcag gaggccctgg tggtagagat gtcctgggac      600

aaaggcgaga ggactgagcc tctcgagaag acagagctac ctgagggtgc ccctgagctg      660

gccctggata cagagttgtc cttggaggat ggagacaggt gcaaggccaa gatg            714


<210>  126
<211>  403
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TGFBR-IL12RB2 receptor

<400>  126

Met Gly Arg Gly Leu Leu Arg Gly Leu Trp Pro Leu His Ile Val Leu 
1               5                   10                  15      


Trp Thr Arg Ile Ala Ser Thr Ile Pro Pro His Val Gln Lys Ser Val 
            20                  25                  30          


Asn Asn Asp Met Ile Val Thr Asp Asn Asn Gly Ala Val Lys Phe Pro 
        35                  40                  45              


Gln Leu Cys Lys Phe Cys Asp Val Arg Phe Ser Thr Cys Asp Asn Gln 
    50                  55                  60                  


Lys Ser Cys Met Ser Asn Cys Ser Ile Thr Ser Ile Cys Glu Lys Pro 
65                  70                  75                  80  


Gln Glu Val Cys Val Ala Val Trp Arg Lys Asn Asp Glu Asn Ile Thr 
                85                  90                  95      


Leu Glu Thr Val Cys His Asp Pro Lys Leu Pro Tyr His Asp Phe Ile 
            100                 105                 110         


Leu Glu Asp Ala Ala Ser Pro Lys Cys Ile Met Lys Glu Lys Lys Lys 
        115                 120                 125             


Pro Gly Glu Thr Phe Phe Met Cys Ser Cys Ser Ser Asp Glu Cys Asn 
    130                 135                 140                 


Asp Asn Ile Ile Phe Ser Glu Glu Tyr Asn Thr Ser Asn Pro Asp Leu 
145                 150                 155                 160 


Leu Leu Val Ile Phe Gln Val Thr Gly Ile Ser Leu Leu Pro Pro Leu 
                165                 170                 175     


Gly Val Ala Ile Ser Val Ile Ile Ile Phe Tyr Gln Gln Lys Val Phe 
            180                 185                 190         


Val Leu Leu Ala Ala Leu Arg Pro Gln Trp Cys Ser Arg Glu Ile Pro 
        195                 200                 205             


Asp Pro Ala Asn Ser Thr Cys Ala Lys Lys Tyr Pro Ile Ala Glu Glu 
    210                 215                 220                 


Lys Thr Gln Leu Pro Leu Asp Arg Leu Leu Ile Asp Trp Pro Thr Pro 
225                 230                 235                 240 


Glu Asp Pro Glu Pro Leu Val Ile Ser Glu Val Leu His Gln Val Thr 
                245                 250                 255     


Pro Val Phe Arg His Pro Pro Cys Ser Asn Trp Pro Gln Arg Glu Lys 
            260                 265                 270         


Gly Ile Gln Gly His Gln Ala Ser Glu Lys Asp Met Met His Ser Ala 
        275                 280                 285             


Ser Ser Pro Pro Pro Pro Arg Ala Leu Gln Ala Glu Ser Arg Gln Leu 
    290                 295                 300                 


Val Asp Leu Tyr Lys Val Leu Glu Ser Arg Gly Ser Asp Pro Lys Pro 
305                 310                 315                 320 


Glu Asn Pro Ala Cys Pro Trp Thr Val Leu Pro Ala Gly Asp Leu Pro 
                325                 330                 335     


Thr His Asp Gly Tyr Leu Pro Ser Asn Ile Asp Asp Leu Pro Ser His 
            340                 345                 350         


Glu Ala Pro Leu Ala Asp Ser Leu Glu Glu Leu Glu Pro Gln His Ile 
        355                 360                 365             


Ser Leu Ser Val Phe Pro Ser Ser Ser Leu His Pro Leu Thr Phe Ser 
    370                 375                 380                 


Cys Gly Asp Lys Leu Thr Leu Asp Gln Leu Lys Met Arg Cys Asp Ser 
385                 390                 395                 400 


Leu Met Leu 
            


<210>  127
<211>  1209
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TGFBR-IL12RB2 receptor

<400>  127
atgggtcggg ggctgctcag gggcctgtgg ccgctgcaca tcgtcctgtg gacgcgtatc       60

gccagcacga tcccaccgca cgttcagaag tcggttaata acgacatgat agtcactgac      120

aacaacggtg cagtcaagtt tccacaactg tgtaaatttt gtgatgtgag attttccacc      180

tgtgacaacc agaaatcctg catgagcaac tgcagcatca cctccatctg tgagaagcca      240

caggaagtct gtgtggctgt atggagaaag aatgacgaga acataacact agagacagtt      300

tgccatgacc ccaagctccc ctaccatgac tttattctgg aagatgctgc ttctccaaag      360

tgcattatga aggaaaaaaa aaagcctggt gagactttct tcatgtgttc ctgtagctct      420

gatgagtgca atgacaacat catcttctca gaagaatata acaccagcaa tcctgacttg      480

ttgctagtca tatttcaagt gacaggcatc agcctcctgc caccactggg agttgccata      540

tctgtcatca tcatcttcta ccagcaaaag gtgtttgttc tcctagcagc cctcagacct      600

cagtggtgta gcagagaaat tccagatcca gcaaatagca cttgcgctaa gaaatatccc      660

attgcagagg agaagacaca gctgcccttg gacaggctcc tgatagactg gcccacgcct      720

gaagatcctg aaccgctggt catcagtgaa gtccttcatc aagtgacccc agttttcaga      780

catcccccct gctccaactg gccacaaagg gaaaaaggaa tccaaggtca tcaggcctct      840

gagaaagaca tgatgcacag tgcctcaagc ccaccacctc caagagctct ccaagctgag      900

agcagacaac tggtggatct gtacaaggtg ctggagagca ggggctccga cccaaagcca      960

gaaaacccag cctgtccctg gacggtgctc ccagcaggtg accttcccac ccatgatggc     1020

tacttaccct ccaacataga tgacctcccc tcacatgagg cacctctcgc tgactctctg     1080

gaagaactgg agcctcagca catctccctt tctgttttcc cctcaagttc tcttcaccca     1140

ctcaccttct cctgtggtga taagctgact ctggatcagt taaagatgag gtgtgactcc     1200

ctcatgctc                                                             1209


<210>  128
<211>  238
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1-CTM-CD28 receptor

<400>  128

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Phe Trp Val Leu Val Val 
                165                 170                 175     


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
            180                 185                 190         


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
        195                 200                 205             


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
    210                 215                 220                 


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
225                 230                 235             


<210>  129
<211>  714
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PD1-CTM-CD28 receptor

<400>  129
atgcagatcc cacaggcgcc ctggccagtc gtctgggcgg tgctacaact gggctggcgg       60

ccaggatggt tcttagactc cccagacagg ccctggaacc cccccacctt ctccccagcc      120

ctgctcgtgg tgaccgaagg ggacaacgcc accttcacct gcagcttctc caacacatcg      180

gagagcttcg tgctaaactg gtaccgcatg agccccagca accagacgga caagctggcc      240

gccttccccg aggaccgcag ccagcccggc caggactgcc gcttccgtgt cacacaactg      300

cccaacgggc gtgacttcca catgagcgtg gtcagggccc ggcgcaatga cagcggcacc      360

tacctctgtg gggccatctc cctggccccc aaggcgcaga tcaaagagag cctgcgggca      420

gagctcaggg tgacagagag aagggcagaa gtgcccacag cccaccccag cccctcaccc      480

aggccagccg gccagttcca aaccctggtg ttttgggtgc tggtggtggt tggtggagtc      540

ctggcttgct atagcttgct agtaacagtg gcctttatta ttttctgggt gaggagtaag      600

aggagcaggc tcctgcacag tgactacatg aacatgactc cccgccgccc cgggcccacc      660

cgcaagcatt accagcccta tgccccacca cgcgacttcg cagcctatcg ctcc            714


<210>  130
<211>  232
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1-PTM-CD28 receptor

<400>  130

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Leu Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Arg 
            180                 185                 190         


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
        195                 200                 205             


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
    210                 215                 220                 


Arg Asp Phe Ala Ala Tyr Arg Ser 
225                 230         


<210>  131
<211>  696
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PD1-PTM-CD28 receptor

<400>  131
atgcagatcc cacaggcgcc ctggccagtc gtctgggcgg tgctacaact gggctggcgg       60

ccaggatggt tcttagactc cccagacagg ccctggaacc cccccacctt ctccccagcc      120

ctgctcgtgg tgaccgaagg ggacaacgcc accttcacct gcagcttctc caacacatcg      180

gagagcttcg tgctaaactg gtaccgcatg agccccagca accagacgga caagctggcc      240

gccttccccg aggaccgcag ccagcccggc caggactgcc gcttccgtgt cacacaactg      300

cccaacgggc gtgacttcca catgagcgtg gtcagggccc ggcgcaatga cagcggcacc      360

tacctctgtg gggccatctc cctggccccc aaggcgcaga tcaaagagag cctgcgggca      420

gagctcaggg tgacagagag aagggcagaa gtgcccacag cccaccccag cccctcaccc      480

aggccagccg gccagttcca aaccctggtg gttggtgtcg tgggcggcct gctgggcagc      540

ctggtgctgc tagtctgggt cctggccgtc atcaggagta agaggagcag gctcctgcac      600

agtgactaca tgaacatgac tccccgccgc cccgggccca cccgcaagca ttaccagccc      660

tatgccccac cacgcgactt cgcagcctat cgctcc                                696


<210>  132
<211>  232
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1A132L-PTM-CD28 receptor

<400>  132

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Leu Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Arg 
            180                 185                 190         


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
        195                 200                 205             


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
    210                 215                 220                 


Arg Asp Phe Ala Ala Tyr Arg Ser 
225                 230         


<210>  133
<211>  693
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PD1A132L-PTM-CD28 receptor

<400>  133
atgcagatcc cacaggcgcc ctggccagtc gtctgggcgg tgctacaact gggctggcgg       60

ccaggatggt tcttagactc cccagacagg ccctggaacc cccccacctt ctccccagcc      120

ctgctcgtgg tgaccgaagg ggacaacgcc accttcacct gcagcttctc caacacatcg      180

gagagcttcg tgctaaactg gtaccgcatg agccccagca accagacgga caagctggcc      240

gccttccccg aggaccgcag ccagcccggc caggactgcc gcttccgtgt cacacaactg      300

cccaacgggc gtgacttcca catgagcgtg gtcagggccc ggcgcaatga cagcggcacc      360

tacctctgtg gggccatctc cctggccccc aagctgcaga tcaaagagag cctgcgggca      420

gagctcaggg tgacagagag aagggcagaa gtgcccacag cccaccccag cccctcaccc      480

aggccagccg gccagttcca aaccctggtg gttggtgtcg tgggcggcct gctgggcagc      540

ctggtgctgc tagtctgggt cctggccgtc atcaggagta agaggagcag gctcctgcac      600

agtgactaca tgaacatgac tccccgccgc cccgggccca cccgcaagca ttaccagccc      660

tatgccccac cacgcgactt cgcagcctat cgc                                   693


<210>  134
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  furin cleavage site


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<400>  134

Arg Xaa Lys Arg 
1               


<210>  135
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  furin cleavage site


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<400>  135

Arg Xaa Arg Arg 
1               


<210>  136
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  furin cleavage site


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is K or R

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa is K or R

<400>  136

Xaa Arg Xaa Xaa Arg 
1               5   


<210>  137
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  furin cleavage site


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  137

Arg Xaa Xaa Arg 
1               


<210>  138
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  furin cleavage site

<400>  138

Arg Gln Lys Arg 
1               


<210>  139
<211>  96
<212>  DNA
<213>  Artificial sequence

<220>
<223>  F-GS2-T2A linker

<400>  139
cgtgcgaaga ggggcggcgg gggctccggc gggggaggca gtgagggccg cggctccctg       60

ctgacctgcg gagatgtaga agagaaccca ggcccc                                 96


<210>  140
<211>  32
<212>  PRT
<213>  Artificial sequence

<220>
<223>  F-GS2-T2A linker

<400>  140

Arg Ala Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Gly 
1               5                   10                  15      


Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
            20                  25                  30          


