                         SEQUENCE LISTING

<110>  Intrexon Corporation
       Thomas, Darby
       Maslowski, John
       Malyala, Anna
 
<120>  DELIVERY OF AUTOLOGOUS CELLS COMPRISING MATRIX METALLOPROTEINASE 
       FOR TREATMENT OF SCLERODERMA

<130>  INX00372WO

<140>  TBD
<141>  2018-04-20

<150>  US 62/488,207
<151>  2017-04-21

<150>  US 62/512,382
<151>  2017-05-30

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1410
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion of nucleotide sequences encoding signal protein sequence 
       from human PEDF (Serpin F1, PEDF-1) gene and human MMP1 (matrix 
       metalloproteinase-1) gene.


<220>
<221>  sig_peptide
<222>  (1)..(57)

<400>  1
atgcaggccc tggtgctact cctctgcatt ggagccctcc tcgggcacag cagctgcttc       60

ccagcgactc tagaaacaca agagcaagat gtggacttag tccagaaata tttggaaaaa      120

tactacaacc tgaagaatga tgggcgccag gttgaaaagc ggagaaatag tggcccagtg      180

gttgaaaaat tgaagcaaat gcaggaattc tttgggctga aagtgactgg gaaaccagat      240

gctgaaaccc tgaaggtgat gaagcagccc agatgtggag tgcctgatgt ggctcagttt      300

gtcctcactg aggggaaccc tcgctgggag caaacacatc tgacctacag gattgaaaat      360

tacacgccag atttgccaag agcagatgtg gaccatgcca ttgagaaagc cttccaactc      420

tggagtaatg tcacacctct gacattcacc aaggtctctg agggtcaagc agacatcatg      480

atcagctttg tcaggggaga tcatcgggac aactctcctt ttgatggacc tggaggaaat      540

cttgctcatg cttttcaacc aggcccaggt attggagggg atgctcattt tgatgaagat      600

gaaaggtgga ccaacaattt cagagagtac aacttacatc gtgttgcagc tcatgaactc      660

ggccattctc ttggactctc ccattctact gatatcgggg ctttgatgta ccctagctac      720

accttcagtg gtgatgttca gctagctcag gatgacattg atggcatcca agccatatat      780

ggacgttccc aaaatcctgt ccagcccatc ggcccacaaa ccccaaaagc gtgtgacagt      840

aagctaacct ttgatgctat aactacgatt cggggagaag tcatgttctt caaagacaga      900

ttctacatgc gcacaaatcc cttctacccg gaagttgagc tcaatttcat ttctgttttc      960

tggccacaac tgccaaatgg gcttgaagct gcttacgaat ttgccgacag agatgaagtc     1020

cggtttttca aagggaataa gtactgggct gttcagggac agaatgtgct acacggatac     1080

cccaaagaca tttacagctc ctttggcttc cctagaactg tgaaacacat tgatgctgct     1140

ctttctgagg aaaacactgg aaaaacctac ttctttgttg ctaacaaata ctggaggtat     1200

gatgaatata aacgatctat ggatccaggt tatcccaaaa tgatagcaca tgactttcct     1260

ggaattggcc acaaagttga tgcagttttc atgaaagatg gatttttcta tttctttcat     1320

ggaacaagac aatacaaatt tgatcctaaa acgaagagaa ttttgactct ccagaaagct     1380

aatagctggt tcaactgcag gaaaaattaa                                      1410


<210>  2
<211>  57
<212>  DNA
<213>  Homo sapiens


<220>
<221>  sig_peptide
<222>  (1)..(57)
<223>  Nucleotide sequence of human PEDF signal peptide

<400>  2
atgcaggccc tggtgctact cctctgcatt ggagccctcc tcgggcacag cagctgc          57


<210>  3
<211>  469
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human PEDF signal peptide and human MMP1 fusion protein


<220>
<221>  SIGNAL
<222>  (1)..(19)

<220>
<221>  PROPEP
<222>  (20)..(99)
<223>  Pre-pro-portion of MMP1 that gets cleaved away to allow enzymatic
       activation of mature polypeptide

<400>  3

Met Gln Ala Leu Val Leu Leu Leu Cys Ile Gly Ala Leu Leu Gly His 
1               5                   10                  15      


Ser Ser Cys Phe Pro Ala Thr Leu Glu Thr Gln Glu Gln Asp Val Asp 
            20                  25                  30          


Leu Val Gln Lys Tyr Leu Glu Lys Tyr Tyr Asn Leu Lys Asn Asp Gly 
        35                  40                  45              


Arg Gln Val Glu Lys Arg Arg Asn Ser Gly Pro Val Val Glu Lys Leu 
    50                  55                  60                  


Lys Gln Met Gln Glu Phe Phe Gly Leu Lys Val Thr Gly Lys Pro Asp 
65                  70                  75                  80  


Ala Glu Thr Leu Lys Val Met Lys Gln Pro Arg Cys Gly Val Pro Asp 
                85                  90                  95      


Val Ala Gln Phe Val Leu Thr Glu Gly Asn Pro Arg Trp Glu Gln Thr 
            100                 105                 110         


His Leu Thr Tyr Arg Ile Glu Asn Tyr Thr Pro Asp Leu Pro Arg Ala 
        115                 120                 125             


Asp Val Asp His Ala Ile Glu Lys Ala Phe Gln Leu Trp Ser Asn Val 
    130                 135                 140                 


Thr Pro Leu Thr Phe Thr Lys Val Ser Glu Gly Gln Ala Asp Ile Met 
145                 150                 155                 160 


Ile Ser Phe Val Arg Gly Asp His Arg Asp Asn Ser Pro Phe Asp Gly 
                165                 170                 175     


Pro Gly Gly Asn Leu Ala His Ala Phe Gln Pro Gly Pro Gly Ile Gly 
            180                 185                 190         


Gly Asp Ala His Phe Asp Glu Asp Glu Arg Trp Thr Asn Asn Phe Arg 
        195                 200                 205             


Glu Tyr Asn Leu His Arg Val Ala Ala His Glu Leu Gly His Ser Leu 
    210                 215                 220                 


Gly Leu Ser His Ser Thr Asp Ile Gly Ala Leu Met Tyr Pro Ser Tyr 
225                 230                 235                 240 


Thr Phe Ser Gly Asp Val Gln Leu Ala Gln Asp Asp Ile Asp Gly Ile 
                245                 250                 255     


Gln Ala Ile Tyr Gly Arg Ser Gln Asn Pro Val Gln Pro Ile Gly Pro 
            260                 265                 270         


Gln Thr Pro Lys Ala Cys Asp Ser Lys Leu Thr Phe Asp Ala Ile Thr 
        275                 280                 285             


Thr Ile Arg Gly Glu Val Met Phe Phe Lys Asp Arg Phe Tyr Met Arg 
    290                 295                 300                 


Thr Asn Pro Phe Tyr Pro Glu Val Glu Leu Asn Phe Ile Ser Val Phe 
305                 310                 315                 320 


Trp Pro Gln Leu Pro Asn Gly Leu Glu Ala Ala Tyr Glu Phe Ala Asp 
                325                 330                 335     


Arg Asp Glu Val Arg Phe Phe Lys Gly Asn Lys Tyr Trp Ala Val Gln 
            340                 345                 350         


Gly Gln Asn Val Leu His Gly Tyr Pro Lys Asp Ile Tyr Ser Ser Phe 
        355                 360                 365             


Gly Phe Pro Arg Thr Val Lys His Ile Asp Ala Ala Leu Ser Glu Glu 
    370                 375                 380                 


Asn Thr Gly Lys Thr Tyr Phe Phe Val Ala Asn Lys Tyr Trp Arg Tyr 
385                 390                 395                 400 


Asp Glu Tyr Lys Arg Ser Met Asp Pro Gly Tyr Pro Lys Met Ile Ala 
                405                 410                 415     


His Asp Phe Pro Gly Ile Gly His Lys Val Asp Ala Val Phe Met Lys 
            420                 425                 430         


Asp Gly Phe Phe Tyr Phe Phe His Gly Thr Arg Gln Tyr Lys Phe Asp 
        435                 440                 445             


Pro Lys Thr Lys Arg Ile Leu Thr Leu Gln Lys Ala Asn Ser Trp Phe 
    450                 455                 460                 


Asn Cys Arg Lys Asn 
465                 


<210>  4
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Gln Ala Leu Val Leu Leu Leu Cys Ile Gly Ala Leu Leu Gly His 
1               5                   10                  15      


Ser Ser Cys 
            


<210>  5
<211>  80
<212>  PRT
<213>  Homo sapiens


<220>
<221>  PEPTIDE
<222>  (1)..(80)
<223>  Pre-pro-MMP1; portion of sequence that gets cleaved away to allow
       enzymatic activation of mature polypeptide

<400>  5

Phe Pro Ala Thr Leu Glu Thr Gln Glu Gln Asp Val Asp Leu Val Gln 
1               5                   10                  15      


Lys Tyr Leu Glu Lys Tyr Tyr Asn Leu Lys Asn Asp Gly Arg Gln Val 
            20                  25                  30          


Glu Lys Arg Arg Asn Ser Gly Pro Val Val Glu Lys Leu Lys Gln Met 
        35                  40                  45              


Gln Glu Phe Phe Gly Leu Lys Val Thr Gly Lys Pro Asp Ala Glu Thr 
    50                  55                  60                  


Leu Lys Val Met Lys Gln Pro Arg Cys Gly Val Pro Asp Val Ala Gln 
65                  70                  75                  80  


<210>  6
<211>  370
<212>  PRT
<213>  Homo sapiens


<220>
<221>  mat_peptide
<222>  (1)..(370)
<223>  Mature (active) MMP1 protein

<400>  6

Phe Val Leu Thr Glu Gly Asn Pro Arg Trp Glu Gln Thr His Leu Thr 
1               5                   10                  15      


Tyr Arg Ile Glu Asn Tyr Thr Pro Asp Leu Pro Arg Ala Asp Val Asp 
            20                  25                  30          


His Ala Ile Glu Lys Ala Phe Gln Leu Trp Ser Asn Val Thr Pro Leu 
        35                  40                  45              


Thr Phe Thr Lys Val Ser Glu Gly Gln Ala Asp Ile Met Ile Ser Phe 
    50                  55                  60                  


Val Arg Gly Asp His Arg Asp Asn Ser Pro Phe Asp Gly Pro Gly Gly 
65                  70                  75                  80  


Asn Leu Ala His Ala Phe Gln Pro Gly Pro Gly Ile Gly Gly Asp Ala 
                85                  90                  95      


His Phe Asp Glu Asp Glu Arg Trp Thr Asn Asn Phe Arg Glu Tyr Asn 
            100                 105                 110         


Leu His Arg Val Ala Ala His Glu Leu Gly His Ser Leu Gly Leu Ser 
        115                 120                 125             


His Ser Thr Asp Ile Gly Ala Leu Met Tyr Pro Ser Tyr Thr Phe Ser 
    130                 135                 140                 


Gly Asp Val Gln Leu Ala Gln Asp Asp Ile Asp Gly Ile Gln Ala Ile 
145                 150                 155                 160 


Tyr Gly Arg Ser Gln Asn Pro Val Gln Pro Ile Gly Pro Gln Thr Pro 
                165                 170                 175     


Lys Ala Cys Asp Ser Lys Leu Thr Phe Asp Ala Ile Thr Thr Ile Arg 
            180                 185                 190         


Gly Glu Val Met Phe Phe Lys Asp Arg Phe Tyr Met Arg Thr Asn Pro 
        195                 200                 205             


Phe Tyr Pro Glu Val Glu Leu Asn Phe Ile Ser Val Phe Trp Pro Gln 
    210                 215                 220                 


Leu Pro Asn Gly Leu Glu Ala Ala Tyr Glu Phe Ala Asp Arg Asp Glu 
225                 230                 235                 240 


Val Arg Phe Phe Lys Gly Asn Lys Tyr Trp Ala Val Gln Gly Gln Asn 
                245                 250                 255     


Val Leu His Gly Tyr Pro Lys Asp Ile Tyr Ser Ser Phe Gly Phe Pro 
            260                 265                 270         


Arg Thr Val Lys His Ile Asp Ala Ala Leu Ser Glu Glu Asn Thr Gly 
        275                 280                 285             


Lys Thr Tyr Phe Phe Val Ala Asn Lys Tyr Trp Arg Tyr Asp Glu Tyr 
    290                 295                 300                 


Lys Arg Ser Met Asp Pro Gly Tyr Pro Lys Met Ile Ala His Asp Phe 
305                 310                 315                 320 


Pro Gly Ile Gly His Lys Val Asp Ala Val Phe Met Lys Asp Gly Phe 
                325                 330                 335     


Phe Tyr Phe Phe His Gly Thr Arg Gln Tyr Lys Phe Asp Pro Lys Thr 
            340                 345                 350         


Lys Arg Ile Leu Thr Leu Gln Lys Ala Asn Ser Trp Phe Asn Cys Arg 
        355                 360                 365             


Lys Asn 
    370 


