                         SEQUENCE LISTING

<110>  AgroSpheres, Inc.
       Shakeel , Ameer Hamza
       Davis , Zachery George
       Frank , Joseph Thomas
       Zomorodi , Sepehr
       Pourtaheri, Payam
 
<120>  COMPOSITIONS AND METHODS FOR ENZYME IMMOBILIZATION

<130>  AGRO-001/01WO 330377-2007

<150>  US 62/491,603
<151>  2017-04-28

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  50
<212>  DNA
<213>  Escherichia coli

<400>  1
cgaagtaaca acaataatgc gtgccataga aattccttgt taaaaaggga                  50


<210>  2
<211>  50
<212>  DNA
<213>  Escherichia coli

<400>  2
cctggcctta ctcaattagc tattaatcat cgccagcgcg cgatgatgtt                  50


<210>  3
<211>  50
<212>  DNA
<213>  Escherichia coli

<400>  3
ttggctgtgt ttttcttccg cgagagaaag aaatcgagta atgccataac                  50


<210>  4
<211>  50
<212>  DNA
<213>  Escherichia coli

<400>  4
agaaattcct tgttaaaaag ggatcaattt aacggttgaa cggtcaaagc                  50


<210>  5
<211>  1773
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AIDA-1 annotated

<400>  5
atgaataagg cctacagtat catttggagc cactccagac aggcctggat tgtggcctca       60

gagttagcca gaggacatgg ttttgtcctt gcaaaaaata cactgctggt attggcggtt      120

gtttccacaa tcggaaatgc atttgcagtc gaccaccatc accatcacca tctggaagcg      180

ctgttccagg gtccgggtac ccagaaacag cgtaccgagc tcgaaaacct gtacttccag      240

ggtgaacaga aactgattag cgaagaagat ctgtctagag tgaataacaa tggaagcatt      300

gtcattaata acagcattat aaacgggaat attacgaatg atgctgactt aagttttggt      360

acagcaaagc tgctctctgc tacagtgaat ggtagtcttg ttaataacaa aaatatcatt      420

cttaatccta caaaagaaag tgcggccgct ataggtaata ctcttaccgt gtcaaattat      480

actgggacac cgggaagtgt tatttctctt ggtggtgtgc ttgaaggaga taattcactt      540

acggaccgtc tggtggtgaa aggtaatacc tctggtcaaa gtgacatcgt ttatgtcaat      600

gaagatggca gtggtggtca gacgagagat ggtattaata ttatttctgt agagggaaat      660

tctgatgcag aattctctct gaagaaccgc gtagttgccg gagcttatga ttacacactg      720

cagaaaggaa acgagagtgg gacagataat aagggatggt atttaaccag tcatcttccc      780

acatctgata cccggcaata cagaccggag aacggaagtt atgctaccaa tatggcactg      840

gctaactcac tgttcctcat ggatttgaat gagcgtaagc aattcagggc catgagtgat      900

aatacacagc ctgagtctgc atccgtgtgg atgaagatca ctggaggaat aagctctggt      960

aagctgaatg acgggcaaaa taaaacaaca accaatcagt ttatcaatca gctcgggggg     1020

gatatttata aattccatgc tgaacaactg ggtgatttta ccttagggat tatgggagga     1080

tacgcgaatg caaaaggtaa aacgataaat tacacgagca acaaagctgc cagaaacaca     1140

ctggatggtt attctgtcgg ggtatacggt acgtggtatc agaatgggga aaatgcaaca     1200

gggctctttg ctgaaacttg gatgcaatat aactggttta atgcatcagt gaaaggtgac     1260

ggactggaag aagaaaaata taatctgaat ggtttaaccg cttctgcagg tgggggatat     1320

aacctgaatg tgcacacatg gacatcacct gaaggaataa caggtgaatt ctggttacag     1380

cctcatttgc aggctgtctg gatgggggtt acaccggata cacatcagga ggataacgga     1440

acggtggtgc agggagcagg gaaaaataat attcagacaa aagcaggtat tcgtgcatcc     1500

tggaaggtga aaagcaccct ggataaggat accgggcgga ggttccgtcc gtatatagag     1560

gcaaactgga tccataacac tcatgaattt ggtgttaaaa tgagtgatga cagccagttg     1620

ttgtcaggta gccgaaatca gggagagata aagacaggta ttgaaggggt gattactcaa     1680

aacttgtcag tgaatggcgg agtcgcatat caggcaggag gtcacgggag caatgccatc     1740

tccggagcac tggggataaa atacagcttc tga                                  1773


<210>  6
<211>  3033
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BrkAutoTransporter_Annotated

<400>  6
atgtatctgg atcgctttcg ccagtgcccg agcagcctgc agattccgcg cagcgcgtgg       60

cgcctgcatg cgctggcggc ggcgctggcg ctggcgggca tggcgcgcct ggcgccggcg      120

gcggcgcagg cgccgcagcc gccggtggcg ggcgcgccgc atgcgcagga tgcgggccag      180

gaaggcgaat ttgatcatcg cgataacacc ctgattgcgg tgtttgatga tggcgtgggc      240

attaacctgg atgatgatcc ggatgaactg ggcgaaaccg cgccgccgac cctgaaagat      300

attcatatta gcgtggaaca taaaaacccg atgagcaaac cggcgattgg cgtgcgcgtg      360

agcggcgcgg gccgcgcgct gaccctggcg ggcagcacca ttgatgcgac cgaaggcggc      420

attccggcgg tggtgcgccg cggcggcacc ctggaactgg atggcgtgac cgtggcgggc      480

ggcgaaggca tggaaccgat gaccgtgagc gatgcgggca gccgcctgag cgtgcgcggc      540

ggcgtgctgg gcggcgaagc gccgggcgtg ggcctggtgc gcgcggcgca gggcggccag      600

gcgagcatta ttgatgcgac cctgcagagc attctgggcc cggcgctgat tgcggatggc      660

ggcagcatta gcgtggcggg cggcagcatt gatatggata tgggcccggg ctttccgccg      720

ccgccgccgc cgctgccggg cgcgccgctg gcggcgcatc cgccgctgga tcgcgtggcg      780

gcggtgcatg cgggccagga tggcaaagtg accctgcgcg aagtggcgct gcgcgcgcat      840

ggcccgcagg cgaccggcgt gtatgcgtat atgccgggca gcgaaattac cctgcagggc      900

ggcaccgtga gcgtgcaggg cgatgatggc gcgggcgtgg tggcgggcgc gggcctgctg      960

gatgcgctgc cgccgggcgg caccgtgcgc ctggatggca ccaccgtgag caccgatggc     1020

gcgaacaccg atgcggtgct ggtgcgcggc gatgcggcgc gcgcggaagt ggtgaacacc     1080

gtgctgcgca ccgcgaaaag cctggcggcg ggcgtgagcg cgcagcatgg cggccgcgtg     1140

accctgcgcc agacccgcat tgaaaccgcg ggcgcgggcg cggaaggcat tagcgtgctg     1200

ggctttgaac cgcagagcgg cagcggcccg gcgagcgtgg atatgcaggg cggcagcatt     1260

accaccaccg gcaaccgcgc ggcgggcatt gcgctgaccc atggcagcgc gcgcctggaa     1320

ggcgtggcgg tgcgcgcgga aggcagcggc agcagcgcgg cgcagctggc gaacggcacc     1380

ctggtggtga gcgcgggcag cctggcgagc gcgcagagcg gcgcgattag cgtgaccgat     1440

accccgctga aactgatgcc gggcgcgctg gcgagcagca ccgtgagcgt gcgcctgacc     1500

gatggcgcga ccgcgcaggg cggcaacggc gtgtttctgc agcagcatag caccattccg     1560

gtggcggtgg cgctggaaag cggcgcgctg gcgcgcggcg atattgtggc ggatggcaac     1620

aaaccgctgg atgcgggcat tagcctgagc gtggcgagcg gcgcggcgtg gcatggcgcg     1680

acccaggtgc tgcagagcgc gaccctgggc aaaggcggca cctgggtggt gaacgcggat     1740

agccgcgtgc aggatatgag catgcgcggc ggccgcgtgg aatttcaggc gccggcgccg     1800

gaagcgagct ataaaaccct gaccctgcag accctggatg gcaacggcgt gtttgtgctg     1860

aacaccaacg tggcggcggg ccagaacgat cagctgcgcg tgaccggccg cgcggatggc     1920

cagcatcgcg tgctggtgcg caacgcgggc ggcgaagcgg atagccgcgg cgcgcgcctg     1980

ggcctggtgc atacccaggg ccagggcaac gcgacctttc gcctggcgaa cgtgggcaaa     2040

gcggtggatc tgggcacctg gcgctatagc ctggcggaag atccgaaaac ccatgtgtgg     2100

agcctgcagc gcgcgggcca ggcgctgagc ggcgcggcga acgcggcggt gaacgcggcg     2160

gatctgagca gcattgcgct ggcggaaagc aacgcgctgg ataaacgcct gggcgaactg     2220

cgcctgcgcg cggatgcggg cggcccgtgg gcgcgcacct ttagcgaacg ccagcagatt     2280

agcaaccgcc atgcgcgcgc gtatgatcag accgtgagcg gcctggaaat tggcctggat     2340

cgcggctgga gcgcgagcgg cggccgctgg tatgcgggcg gcctgctggg ctatacctat     2400

gcggatcgca cctatccggg cgatggcggc ggcaaagtga aaggcctgca tgtgggcggc     2460

tatgcggcgt atgtgggcga tggcggctat tatctggata ccgtgctgcg cctgggccgc     2520

tatgatcagc agtataacat tgcgggcacc gatggcggcc gcgtgaccgc ggattatcgc     2580

accagcggcg cggcgtggag cctggaaggc ggccgccgct ttgaactgcc gaacgattgg     2640

tttgcggaac cgcaggcgga agtgatgctg tggcgcacca gcggcaaacg ctatcgcgcg     2700

agcaacggcc tgcgcgtgaa agtggatgcg aacaccgcga ccctgggccg cctgggcctg     2760

cgctttggcc gccgcattgc gctggcgggc ggcaacattg tgcagccgta tgcgcgcctg     2820

ggctggaccc aggaatttaa aagcaccggc gatgtgcgca ccaacggcat tggccatgcg     2880

ggcgcgggcc gccatggccg cgtggaactg ggcgcgggcg tggatgcggc gctgggcaaa     2940

ggccataacc tgtatgcgag ctatgaatat gcggcgggcg atcgcattaa cattccgtgg     3000

agctttcatg cgggctatcg ctatagcttt tga                                  3033


<210>  7
<211>  2343
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BrkAutoTransporter_CBM

<400>  7
atgtatctgg atcgctttcg ccagtgcccg agcagcctgc agattccgcg cagcgcgtgg       60

cgcctgcatg cgctggcggc ggcgctggcg ctggcgggca tggcgcgcct ggcgccggcg      120

gcggcgcagg cgccgcagcc gccggtggcg ggcgcgccgc atgcgcagga tgcgggccac      180

catcaccatc accatgttca gctggttgaa agcggtggtg cactggttca gcctggtggt      240

agcctgcgtc tgagctgtgc agcaagcggt tttccggtta atcgttatag catgcgttgg      300

tatcgtcagg caccgggtaa agaacgtgaa tgggttgcag gtatgagcag tgccggtgat      360

cgtagcagct acgaagatag cgttaaaggt cgttttacca tcagccgtga tgatgcacgt      420

aataccgttt atctgcaaat gaatagcctg aaaccggaag ataccgcagt gtattattgc      480

aacgttaacg tgggctttga atattggggt cagggcaccc aggttaccgt tagcagcaaa      540

ctcgagcggc cgcatcgtga cgcgtcgtct ggtcctgccg gctgccaagt cctttggggc      600

gtgaatcagt ggaacacagg tttcacggcg aatgttaccg tcaagaatac gtcctccgct      660

cctgttgacg gctggacctt gaccttcagt ttcccatcag gacaacaagt cactcaagcc      720

tggtcatcta ccgtgaccca gagtggatct gcggtcacag tacgtaacgc tccgtggaac      780

ggttcgattc ccgcgggcgg gactgctcag ttcgggttta acggaagcca cactggcact      840

aatgctgcac caactgcctt ctcacttaac ggcacgccgt gcaccgtagg cgaacagaaa      900

ctgattagcg aagaagatct ggaaaacctg tacttccagg gtgcgggcat tagcctgagc      960

gtggcgagcg gcgcggcgtg gcatggcgcg acccaggtgc tgcagagcgc gaccctgggc     1020

aaaggcggca cctgggtggt gaacgcggat agccgcgtgc aggatatgag catgcgcggc     1080

ggccgcgtgg aatttcaggc gccggcgccg gaagcgagct ataaaaccct gaccctgcag     1140

accctggatg gcaacggcgt gtttgtgctg aacaccaacg tggcggcggg ccagaacgat     1200

cagctgcgcg tgaccggccg cgcggatggc cagcatcgcg tgctggtgcg caacgcgggc     1260

ggcgaagcgg atagccgcgg cgcgcgcctg ggcctggtgc atacccaggg ccagggcaac     1320

gcgacctttc gcctggcgaa cgtgggcaaa gcggtggatc tgggcacctg gcgctatagc     1380

ctggcggaag atccgaaaac ccatgtgtgg agcctgcagc gcgcgggcca ggcgctgagc     1440

ggcgcggcga acgcggcggt gaacgcggcg gatctgagca gcattgcgct ggcggaaagc     1500

aacgcgctgg ataaacgcct gggcgaactg cgcctgcgcg cggatgcggg cggcccgtgg     1560

gcgcgcacct ttagcgaacg ccagcagatt agcaaccgcc atgcgcgcgc gtatgatcag     1620

accgtgagcg gcctggaaat tggcctggat cgcggctgga gcgcgagcgg cggccgctgg     1680

tatgcgggcg gcctgctggg ctatacctat gcggatcgca cctatccggg cgatggcggc     1740

ggcaaagtga aaggcctgca tgtgggcggc tatgcggcgt atgtgggcga tggcggctat     1800

tatctggata ccgtgctgcg cctgggccgc tatgatcagc agtataacat tgcgggcacc     1860

gatggcggcc gcgtgaccgc ggattatcgc accagcggcg cggcgtggag cctggaaggc     1920

ggccgccgct ttgaactgcc gaacgattgg tttgcggaac cgcaggcgga agtgatgctg     1980

tggcgcacca gcggcaaacg ctatcgcgcg agcaacggcc tgcgcgtgaa agtggatgcg     2040

aacaccgcga ccctgggccg cctgggcctg cgctttggcc gccgcattgc gctggcgggc     2100

ggcaacattg tgcagccgta tgcgcgcctg ggctggaccc aggaatttaa aagcaccggc     2160

gatgtgcgca ccaacggcat tggccatgcg ggcgcgggcc gccatggccg cgtggaactg     2220

ggcgcgggcg tggatgcggc gctgggcaaa ggccataacc tgtatgcgag ctatgaatat     2280

gcggcgggcg atcgcattaa cattccgtgg agctttcatg cgggctatcg ctatagcttt     2340

tga                                                                   2343


<210>  8
<211>  3258
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BrkAutoTransporter_Lipase

<400>  8
atgtatctgg atcgctttcg ccagtgcccg agcagcctgc agattccgcg cagcgcgtgg       60

cgcctgcatg cgctggcggc ggcgctggcg ctggcgggca tggcgcgcct ggcgccggcg      120

gcggcgcagg cgccgcagcc gccggtggcg ggcgcgccgc atgcgcagga tgcgggccac      180

catcaccatc accatgttca gctggttgaa agcggtggtg cactggttca gcctggtggt      240

agcctgcgtc tgagctgtgc agcaagcggt tttccggtta atcgttatag catgcgttgg      300

tatcgtcagg caccgggtaa agaacgtgaa tgggttgcag gtatgagcag tgccggtgat      360

cgtagcagct acgaagatag cgttaaaggt cgttttacca tcagccgtga tgatgcacgt      420

aataccgttt atctgcaaat gaatagcctg aaaccggaag ataccgcagt gtattattgc      480

aacgttaacg tgggctttga atattggggt cagggcaccc aggttaccgt tagcagcaaa      540

ctcgagcggc cgcatcgtga caagtgctgt cggattatgt ttgtgttgct cggactgtgg      600

tttgtgttcg gcctatcggt cccgggaggg cggacggaag cggcatccct acgcgccaac      660

gacgcaccga ttgtgcttct ccacgggttt accggctggg gacgagagga aatgtttgga      720

ttcaagtatt ggggcggcgt gcgcggcgat atcgaacagt ggctgaacga caacggttat      780

cgaacgtata cgctggcggt cggaccgctc tcgagcaact gggaccgggc gtgtgaagcg      840

tacgctcagc ttgtcggcgg gacggtcgat tacggggcag cccacgcggc aaagcacggc      900

cacgcgcggt ttggccgcac ttatcccggc ctgttgccgg aattgaaaag gggtggccgc      960

atccatatca tcgcccacag ccaagggggg cagacggccc gcatgcttgt ctcgctccta     1020

gagaacggaa gccaagaaga gcgggagtac gccaaggcgc ataacgtgtc gttgtcaccg     1080

ttgtttgaag gtggacatca ttttgtgttg agtgtgacga ccatcgccac tcctcacgac     1140

gggacgacgc ttgtcaacat ggttgatttc accgatcgct tttttgactt gcaaaaagcg     1200

gtgttggaag cggcggctgt cgccagcaac gtgccgtaca cgagtcaagt atacgatttt     1260

aagctcgacc aatggggact gcgccgccag ccgggtgaat cgttcgacca ttattttgaa     1320

cggctcaagc gctcccctgt ttggacgtcc acagataccg cccgctacga tttatccgtt     1380

tccggagctg agaagctcaa tcagtgggtg caagcaagcc cgaatacgta ttatttgagt     1440

ttctctacag aacggacgta tcgcggagcg ctcacaggca accattatcc cgaactcgga     1500

atgaacgcat tcagcgcggt cgtgtgcgct ccgtttctcg gttcgtaccg caatccgacg     1560

ctcggcattg acgaccgttg gttggagaac gatggcattg tcaatacggt ttccatgaac     1620

ggtccaaagc gtggatcaag cgatcggatc gtgccgtatg acgggacgtt gaaaaaaggg     1680

gtttggaacg atatgggaac gtacaacgtc gaccatttgg aaatcatcgg cgttgacccg     1740

aatccgtcat ttgatattcg cgccttttat ttgcggcttg ccgagcagtt ggcgagcttg     1800

cggcctgaac agaaactgat tagcgaagaa gatctggaaa acctgtactt ccagggtgcg     1860

ggcattagcc tgagcgtggc gagcggcgcg gcgtggcatg gcgcgaccca ggtgctgcag     1920

agcgcgaccc tgggcaaagg cggcacctgg gtggtgaacg cggatagccg cgtgcaggat     1980

atgagcatgc gcggcggccg cgtggaattt caggcgccgg cgccggaagc gagctataaa     2040

accctgaccc tgcagaccct ggatggcaac ggcgtgtttg tgctgaacac caacgtggcg     2100

gcgggccaga acgatcagct gcgcgtgacc ggccgcgcgg atggccagca tcgcgtgctg     2160

gtgcgcaacg cgggcggcga agcggatagc cgcggcgcgc gcctgggcct ggtgcatacc     2220

cagggccagg gcaacgcgac ctttcgcctg gcgaacgtgg gcaaagcggt ggatctgggc     2280

acctggcgct atagcctggc ggaagatccg aaaacccatg tgtggagcct gcagcgcgcg     2340

ggccaggcgc tgagcggcgc ggcgaacgcg gcggtgaacg cggcggatct gagcagcatt     2400

gcgctggcgg aaagcaacgc gctggataaa cgcctgggcg aactgcgcct gcgcgcggat     2460

gcgggcggcc cgtgggcgcg cacctttagc gaacgccagc agattagcaa ccgccatgcg     2520

cgcgcgtatg atcagaccgt gagcggcctg gaaattggcc tggatcgcgg ctggagcgcg     2580

agcggcggcc gctggtatgc gggcggcctg ctgggctata cctatgcgga tcgcacctat     2640

ccgggcgatg gcggcggcaa agtgaaaggc ctgcatgtgg gcggctatgc ggcgtatgtg     2700

ggcgatggcg gctattatct ggataccgtg ctgcgcctgg gccgctatga tcagcagtat     2760

aacattgcgg gcaccgatgg cggccgcgtg accgcggatt atcgcaccag cggcgcggcg     2820

tggagcctgg aaggcggccg ccgctttgaa ctgccgaacg attggtttgc ggaaccgcag     2880

gcggaagtga tgctgtggcg caccagcggc aaacgctatc gcgcgagcaa cggcctgcgc     2940

gtgaaagtgg atgcgaacac cgcgaccctg ggccgcctgg gcctgcgctt tggccgccgc     3000

attgcgctgg cgggcggcaa cattgtgcag ccgtatgcgc gcctgggctg gacccaggaa     3060

tttaaaagca ccggcgatgt gcgcaccaac ggcattggcc atgcgggcgc gggccgccat     3120

ggccgcgtgg aactgggcgc gggcgtggat gcggcgctgg gcaaaggcca taacctgtat     3180

gcgagctatg aatatgcggc gggcgatcgc attaacattc cgtggagctt tcatgcgggc     3240

tatcgctata gcttttga                                                   3258


<210>  9
<211>  330
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CBM

<400>  9
gcgtcgtctg gtcctgccgg ctgccaagtc ctttggggcg tgaatcagtg gaacacaggt       60

ttcacggcga atgttaccgt caagaatacg tcctccgctc ctgttgacgg ctggaccttg      120

accttcagtt tcccatcagg acaacaagtc actcaagcct ggtcatctac cgtgacccag      180

agtggatctg cggtcacagt acgtaacgct ccgtggaacg gttcgattcc cgcgggcggg      240

actgctcagt tcgggtttaa cggaagccac actggcacta atgctgcacc aactgccttc      300

tcacttaacg gcacgccgtg caccgtaggc                                       330


<210>  10
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GFP-Nanobody CBM

<400>  10
gttcagctgg ttgaaagcgg tggtgcactg gttcagcctg gtggtagcct gcgtctgagc       60

tgtgcagcaa gcggttttcc ggttaatcgt tatagcatgc gttggtatcg tcaggcaccg      120

ggtaaagaac gtgaatgggt tgcaggtatg agcagtgccg gtgatcgtag cagctacgaa      180

gatagcgtta aaggtcgttt taccatcagc cgtgatgatg cacgtaatac cgtttatctg      240

caaatgaata gcctgaaacc ggaagatacc gcagtgtatt attgcaacgt taacgtgggc      300

tttgaatatt ggggtcaggg cacccaggtt accgttagca gcaaactcga gcggccgcat      360

cgtgacgcgt cgtctggtcc tgccggctgc caagtccttt ggggcgtgaa tcagtggaac      420

acaggtttca cggcgaatgt taccgtcaag aatacgtcct ccgctcctgt tgacggctgg      480

accttgacct tcagtttccc atcaggacaa caagtcactc aagcctggtc atctaccgtg      540

acccagagtg gatctgcggt cacagtacgt aacgctccgt ggaacggttc gattcccgcg      600

ggcgggactg ctcagttcgg gtttaacgga agccacactg gcactaatgc tgcaccaact      660

gccttctcac ttaacggcac gccgtgcacc gtaggc                                696


<210>  11
<211>  1611
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GFP-Nanobody Lipase

<400>  11
gttcagctgg ttgaaagcgg tggtgcactg gttcagcctg gtggtagcct gcgtctgagc       60

tgtgcagcaa gcggttttcc ggttaatcgt tatagcatgc gttggtatcg tcaggcaccg      120

ggtaaagaac gtgaatgggt tgcaggtatg agcagtgccg gtgatcgtag cagctacgaa      180

gatagcgtta aaggtcgttt taccatcagc cgtgatgatg cacgtaatac cgtttatctg      240

caaatgaata gcctgaaacc ggaagatacc gcagtgtatt attgcaacgt taacgtgggc      300

tttgaatatt ggggtcaggg cacccaggtt accgttagca gcaaactcga gcggccgcat      360

cgtgacaagt gctgtcggat tatgtttgtg ttgctcggac tgtggtttgt gttcggccta      420

tcggtcccgg gagggcggac ggaagcggca tccctacgcg ccaacgacgc accgattgtg      480

cttctccacg ggtttaccgg ctggggacga gaggaaatgt ttggattcaa gtattggggc      540

ggcgtgcgcg gcgatatcga acagtggctg aacgacaacg gttatcgaac gtatacgctg      600

gcggtcggac cgctctcgag caactgggac cgggcgtgtg aagcgtacgc tcagcttgtc      660

ggcgggacgg tcgattacgg ggcagcccac gcggcaaagc acggccacgc gcggtttggc      720

cgcacttatc ccggcctgtt gccggaattg aaaaggggtg gccgcatcca tatcatcgcc      780

cacagccaag gggggcagac ggcccgcatg cttgtctcgc tcctagagaa cggaagccaa      840

gaagagcggg agtacgccaa ggcgcataac gtgtcgttgt caccgttgtt tgaaggtgga      900

catcattttg tgttgagtgt gacgaccatc gccactcctc acgacgggac gacgcttgtc      960

aacatggttg atttcaccga tcgctttttt gacttgcaaa aagcggtgtt ggaagcggcg     1020

gctgtcgcca gcaacgtgcc gtacacgagt caagtatacg attttaagct cgaccaatgg     1080

ggactgcgcc gccagccggg tgaatcgttc gaccattatt ttgaacggct caagcgctcc     1140

cctgtttgga cgtccacaga taccgcccgc tacgatttat ccgtttccgg agctgagaag     1200

ctcaatcagt gggtgcaagc aagcccgaat acgtattatt tgagtttctc tacagaacgg     1260

acgtatcgcg gagcgctcac aggcaaccat tatcccgaac tcggaatgaa cgcattcagc     1320

gcggtcgtgt gcgctccgtt tctcggttcg taccgcaatc cgacgctcgg cattgacgac     1380

cgttggttgg agaacgatgg cattgtcaat acggtttcca tgaacggtcc aaagcgtgga     1440

tcaagcgatc ggatcgtgcc gtatgacggg acgttgaaaa aaggggtttg gaacgatatg     1500

ggaacgtaca acgtcgacca tttggaaatc atcggcgttg acccgaatcc gtcatttgat     1560

attcgcgcct tttatttgcg gcttgccgag cagttggcga gcttgcggcc t              1611


<210>  12
<211>  537
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  InaK

<400>  12
atggtcttag acaaggcgct agttctacgt acctgcgcta ataatatggc cgatcactgc       60

ggcttgattt ggcctgcctc agggaccgtc gagtcaaggt attggcaatc tacacgtcgt      120

cacgagaacg gactggtagg tcttctttgg ggagcaggaa cttctgcttt cttgtcagtc      180

catgcagacg cccgctggat cgtgtgcgaa gtggctgttg ccgatattat ctccctagag      240

gagcccggaa tggttaaatt tcctcgggcc gaagtggtgc atgtgggcga tcgaatcagc      300

gcttctcatt ttatttcggc gcggcaggca gatcccgcga gtacgagtac ttcaacgtcg      360

acaagtactc ttactcccat gcccacggca atccccaccc ctatgccagc ggtggcgtca      420

gtgacgttac cggtggctga gcaagcgcgg catgaggtgt ttgatgtagc tagtgtgagc      480

gccgcggctg ctcccgtgaa cactttacct gtcacgacac cccaaaacct ccagacg         537


<210>  13
<211>  1305
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  InaK-TEV-CBM-His-Myc-GFPNB

<400>  13
atggtcttag acaaggcgct agttctacgt acctgcgcta ataatatggc cgatcactgc       60

ggcttgattt ggcctgcctc agggaccgtc gagtcaaggt attggcaatc tacacgtcgt      120

cacgagaacg gactggtagg tcttctttgg ggagcaggaa cttctgcttt cttgtcagtc      180

catgcagacg cccgctggat cgtgtgcgaa gtggctgttg ccgatattat ctccctagag      240

gagcccggaa tggttaaatt tcctcgggcc gaagtggtgc atgtgggcga tcgaatcagc      300

gcttctcatt ttatttcggc gcggcaggca gatcccgcga gtacgagtac ttcaacgtcg      360

acaagtactc ttactcccat gcccacggca atccccaccc ctatgccagc ggtggcgtca      420

gtgacgttac cggtggctga gcaagcgcgg catgaggtgt ttgatgtagc tagtgtgagc      480

gccgcggctg ctcccgtgaa cactttacct gtcacgacac cccaaaacct ccagacggaa      540

aacctgtact tccagggtgc gtcgtctggt cctgccggct gccaagtcct ttggggcgtg      600

aatcagtgga acacaggttt cacggcgaat gttaccgtca agaatacgtc ctccgctcct      660

gttgacggct ggaccttgac cttcagtttc ccatcaggac aacaagtcac tcaagcctgg      720

tcatctaccg tgacccagag tggatctgcg gtcacagtac gtaacgctcc gtggaacggt      780

tcgattcccg cgggcgggac tgctcagttc gggtttaacg gaagccacac tggcactaat      840

gctgcaccaa ctgccttctc acttaacggc acgccgtgca ccgtaggcca ccatcaccat      900

caccatgttc agctggttga aagcggtggt gcactggttc agcctggtgg tagcctgcgt      960

ctgagctgtg cagcaagcgg ttttccggtt aatcgttata gcatgcgttg gtatcgtcag     1020

gcaccgggta aagaacgtga atgggttgca ggtatgagca gtgccggtga tcgtagcagc     1080

tacgaagata gcgttaaagg tcgttttacc atcagccgtg atgatgcacg taataccgtt     1140

tatctgcaaa tgaatagcct gaaaccggaa gataccgcag tgtattattg caacgttaac     1200

gtgggctttg aatattgggg tcagggcacc caggttaccg ttagcagcaa actcgagcgg     1260

ccgcatcgtg acgaacagaa actgattagc gaagaagatc tgtga                     1305


<210>  14
<211>  2220
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  InaK-TEV-Lipase-His-Myc-GFPNB

<400>  14
atggtcttag acaaggcgct agttctacgt acctgcgcta ataatatggc cgatcactgc       60

ggcttgattt ggcctgcctc agggaccgtc gagtcaaggt attggcaatc tacacgtcgt      120

cacgagaacg gactggtagg tcttctttgg ggagcaggaa cttctgcttt cttgtcagtc      180

catgcagacg cccgctggat cgtgtgcgaa gtggctgttg ccgatattat ctccctagag      240

gagcccggaa tggttaaatt tcctcgggcc gaagtggtgc atgtgggcga tcgaatcagc      300

gcttctcatt ttatttcggc gcggcaggca gatcccgcga gtacgagtac ttcaacgtcg      360

acaagtactc ttactcccat gcccacggca atccccaccc ctatgccagc ggtggcgtca      420

gtgacgttac cggtggctga gcaagcgcgg catgaggtgt ttgatgtagc tagtgtgagc      480

gccgcggctg ctcccgtgaa cactttacct gtcacgacac cccaaaacct ccagacggaa      540

aacctgtact tccagggtaa gtgctgtcgg attatgtttg tgttgctcgg actgtggttt      600

gtgttcggcc tatcggtccc gggagggcgg acggaagcgg catccctacg cgccaacgac      660

gcaccgattg tgcttctcca cgggtttacc ggctggggac gagaggaaat gtttggattc      720

aagtattggg gcggcgtgcg cggcgatatc gaacagtggc tgaacgacaa cggttatcga      780

acgtatacgc tggcggtcgg accgctctcg agcaactggg accgggcgtg tgaagcgtac      840

gctcagcttg tcggcgggac ggtcgattac ggggcagccc acgcggcaaa gcacggccac      900

gcgcggtttg gccgcactta tcccggcctg ttgccggaat tgaaaagggg tggccgcatc      960

catatcatcg cccacagcca aggggggcag acggcccgca tgcttgtctc gctcctagag     1020

aacggaagcc aagaagagcg ggagtacgcc aaggcgcata acgtgtcgtt gtcaccgttg     1080

tttgaaggtg gacatcattt tgtgttgagt gtgacgacca tcgccactcc tcacgacggg     1140

acgacgcttg tcaacatggt tgatttcacc gatcgctttt ttgacttgca aaaagcggtg     1200

ttggaagcgg cggctgtcgc cagcaacgtg ccgtacacga gtcaagtata cgattttaag     1260

ctcgaccaat ggggactgcg ccgccagccg ggtgaatcgt tcgaccatta ttttgaacgg     1320

ctcaagcgct cccctgtttg gacgtccaca gataccgccc gctacgattt atccgtttcc     1380

ggagctgaga agctcaatca gtgggtgcaa gcaagcccga atacgtatta tttgagtttc     1440

tctacagaac ggacgtatcg cggagcgctc acaggcaacc attatcccga actcggaatg     1500

aacgcattca gcgcggtcgt gtgcgctccg tttctcggtt cgtaccgcaa tccgacgctc     1560

ggcattgacg accgttggtt ggagaacgat ggcattgtca atacggtttc catgaacggt     1620

ccaaagcgtg gatcaagcga tcggatcgtg ccgtatgacg ggacgttgaa aaaaggggtt     1680

tggaacgata tgggaacgta caacgtcgac catttggaaa tcatcggcgt tgacccgaat     1740

ccgtcatttg atattcgcgc cttttatttg cggcttgccg agcagttggc gagcttgcgg     1800

cctcaccatc accatcacca tgttcagctg gttgaaagcg gtggtgcact ggttcagcct     1860

ggtggtagcc tgcgtctgag ctgtgcagca agcggttttc cggttaatcg ttatagcatg     1920

cgttggtatc gtcaggcacc gggtaaagaa cgtgaatggg ttgcaggtat gagcagtgcc     1980

ggtgatcgta gcagctacga agatagcgtt aaaggtcgtt ttaccatcag ccgtgatgat     2040

gcacgtaata ccgtttatct gcaaatgaat agcctgaaac cggaagatac cgcagtgtat     2100

tattgcaacg ttaacgtggg ctttgaatat tggggtcagg gcacccaggt taccgttagc     2160

agcaaactcg agcggccgca tcgtgacgaa cagaaactga ttagcgaaga agatctgtga     2220


<210>  15
<211>  1245
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bacillus sp. 42 thermostable organic solvent tolerant lipase gene
       annotated

<400>  15
aagtgctgtc ggattatgtt tgtgttgctc ggactgtggt ttgtgttcgg cctatcggtc       60

ccgggagggc ggacggaagc ggcatcccta cgcgccaacg acgcaccgat tgtgcttctc      120

cacgggttta ccggctgggg acgagaggaa atgtttggat tcaagtattg gggcggcgtg      180

cgcggcgata tcgaacagtg gctgaacgac aacggttatc gaacgtatac gctggcggtc      240

ggaccgctct cgagcaactg ggaccgggcg tgtgaagcgt acgctcagct tgtcggcggg      300

acggtcgatt acggggcagc ccacgcggca aagcacggcc acgcgcggtt tggccgcact      360

tatcccggcc tgttgccgga attgaaaagg ggtggccgca tccatatcat cgcccacagc      420

caaggggggc agacggcccg catgcttgtc tcgctcctag agaacggaag ccaagaagag      480

cgggagtacg ccaaggcgca taacgtgtcg ttgtcaccgt tgtttgaagg tggacatcat      540

tttgtgttga gtgtgacgac catcgccact cctcacgacg ggacgacgct tgtcaacatg      600

gttgatttca ccgatcgctt ttttgacttg caaaaagcgg tgttggaagc ggcggctgtc      660

gccagcaacg tgccgtacac gagtcaagta tacgatttta agctcgacca atggggactg      720

cgccgccagc cgggtgaatc gttcgaccat tattttgaac ggctcaagcg ctcccctgtt      780

tggacgtcca cagataccgc ccgctacgat ttatccgttt ccggagctga gaagctcaat      840

cagtgggtgc aagcaagccc gaatacgtat tatttgagtt tctctacaga acggacgtat      900

cgcggagcgc tcacaggcaa ccattatccc gaactcggaa tgaacgcatt cagcgcggtc      960

gtgtgcgctc cgtttctcgg ttcgtaccgc aatccgacgc tcggcattga cgaccgttgg     1020

ttggagaacg atggcattgt caatacggtt tccatgaacg gtccaaagcg tggatcaagc     1080

gatcggatcg tgccgtatga cgggacgttg aaaaaagggg tttggaacga tatgggaacg     1140

tacaacgtcg accatttgga aatcatcggc gttgacccga atccgtcatt tgatattcgc     1200

gccttttatt tgcggcttgc cgagcagttg gcgagcttgc ggcct                     1245


<210>  16
<211>  5561
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAIDA-1

<400>  16
tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca       60

cacaggaaag cttcatatga ataaggccta cagtatcatt tggagccact ccagacaggc      120

ctggattgtg gcctcagagt tagccagagg acatggtttt gtccttgcaa aaaatacact      180

gctggtattg gcggttgttt ccacaatcgg aaatgcattt gcagtcgacc accatcacca      240

tcaccatctg gaagcgctgt tccagggtcc gggtacccag aaacagcgta ccgagctcga      300

aaacctgtac ttccagggtg aacagaaact gattagcgaa gaagatctgt ctagagtgaa      360

taacaatgga agcattgtca ttaataacag cattataaac gggaatatta cgaatgatgc      420

tgacttaagt tttggtacag caaagctgct ctctgctaca gtgaatggta gtcttgttaa      480

taacaaaaat atcattctta atcctacaaa agaaagtgcg gccgctatag gtaatactct      540

taccgtgtca aattatactg ggacaccggg aagtgttatt tctcttggtg gtgtgcttga      600

aggagataat tcacttacgg accgtctggt ggtgaaaggt aatacctctg gtcaaagtga      660

catcgtttat gtcaatgaag atggcagtgg tggtcagacg agagatggta ttaatattat      720

ttctgtagag ggaaattctg atgcagaatt ctctctgaag aaccgcgtag ttgccggagc      780

ttatgattac acactgcaga aaggaaacga gagtgggaca gataataagg gatggtattt      840

aaccagtcat cttcccacat ctgatacccg gcaatacaga ccggagaacg gaagttatgc      900

taccaatatg gcactggcta actcactgtt cctcatggat ttgaatgagc gtaagcaatt      960

cagggccatg agtgataata cacagcctga gtctgcatcc gtgtggatga agatcactgg     1020

aggaataagc tctggtaagc tgaatgacgg gcaaaataaa acaacaacca atcagtttat     1080

caatcagctc gggggggata tttataaatt ccatgctgaa caactgggtg attttacctt     1140

agggattatg ggaggatacg cgaatgcaaa aggtaaaacg ataaattaca cgagcaacaa     1200

agctgccaga aacacactgg atggttattc tgtcggggta tacggtacgt ggtatcagaa     1260

tggggaaaat gcaacagggc tctttgctga aacttggatg caatataact ggtttaatgc     1320

atcagtgaaa ggtgacggac tggaagaaga aaaatataat ctgaatggtt taaccgcttc     1380

tgcaggtggg ggatataacc tgaatgtgca cacatggaca tcacctgaag gaataacagg     1440

tgaattctgg ttacagcctc atttgcaggc tgtctggatg ggggttacac cggatacaca     1500

tcaggaggat aacggaacgg tggtgcaggg agcagggaaa aataatattc agacaaaagc     1560

aggtattcgt gcatcctgga aggtgaaaag caccctggat aaggataccg ggcggaggtt     1620

ccgtccgtat atagaggcaa actggatcca taacactcat gaatttggtg ttaaaatgag     1680

tgatgacagc cagttgttgt caggtagccg aaatcaggga gagataaaga caggtattga     1740

aggggtgatt actcaaaact tgtcagtgaa tggcggagtc gcatatcagg caggaggtca     1800

cgggagcaat gccatctccg gagcactggg gataaaatac agcttctgat aatgatcctg     1860

gcacgcggcg cgccccttgg tgcgcaaact attaactggc gaactactta ctctagcttc     1920

ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc     1980

ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg     2040

cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac     2100

gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc     2160

actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt     2220

aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac     2280

caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccttaa taagatgatc     2340

ttcttgagat cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt     2400

gcagggcggt ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct     2460

tggaggagcg cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt     2520

caagactaac tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc     2580

tttccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg gactgaacgg     2640

ggggttcgtg catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc     2700

gtggaatgag acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga     2760

acaggagagc gcacgaggga gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc     2820

gggtttcgcc accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc     2880

ctatggaaaa acggctttgc cgcggccctc tcacttccct gttaagtatc ttcctggcat     2940

cttccaggaa atctccgccc cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg     3000

agcgtagcga gtcagtgagc gaggaagcgg aatatatcct gtatcacata ttctgctgac     3060

gcaccggtgc agcctttttt ctcctgccac atgaagcact tcactgacac cctcatcagt     3120

gccaacatag taagccagta tacactccgc tagcgctgag gtctgcctcg tgaagaaggt     3180

gttgctgact cataccaggc ctgaatcgcc ccatcatcca gccagaaagt gagggagcca     3240

cggttgatga gagctttgtt gtaggtggac cagttggtga ttttgaactt ttgctttgcc     3300

acggaacggt ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc agcaaaagtt     3360

cgatttattc aacaaagcca cgttgtgtct caaaatctct gatgttacat tgcacaagat     3420

aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt     3480

gttatgagcc atattcaacg ggaaacgtct tgctcgagta tccgctcatg agattatcaa     3540

aaaggatctt cacctagatc cttttgtaag aggttccaac tttcaccata atgaaataag     3600

atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat     3660

ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca     3720

ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat     3780

tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca     3840

cattcttgcc cgcctgatga atgctcatcc ggagtttcgt atggcaatga aagacggtga     3900

gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac     3960

gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc     4020

gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa     4080

tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc     4140

caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga     4200

caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt     4260

cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt     4320

tttttaaggc gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc     4380

ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga     4440

gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc     4500

tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg     4560

cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct     4620

ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg     4680

tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt     4740

gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca     4800

ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc     4860

tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt     4920

ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc     4980

tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca     5040

gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca     5100

aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct     5160

gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt     5220

gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca     5280

ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca     5340

ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc     5400

gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg     5460

acaggtttcc cgactggaaa gcgggcaagt gagtggataa ccgtattacc gcctttgagt     5520

gagctgatac cgggaattct cactcattag gcaccccagg c                         5561


<210>  17
<211>  6242
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAIDA-1_CBM-Nanobody

<400>  17
tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca       60

cacaggaaag cttcatatga ataaggccta cagtatcatt tggagccact ccagacaggc      120

ctggattgtg gcctcagagt tagccagagg acatggtttt gtccttgcaa aaaatacact      180

gctggtattg gcggttgttt ccacaatcgg aaatgcattt gcagtcgacc accatcacca      240

tcaccatctg gaagcgctgt tccagggtcc gggtaccgtt cagctggttg aaagcggtgg      300

tgcactggtt cagcctggtg gtagcctgcg tctgagctgt gcagcaagcg gttttccggt      360

taatcgttat agcatgcgtt ggtatcgtca ggcaccgggt aaagaacgtg aatgggttgc      420

aggtatgagc agtgccggtg atcgtagcag ctacgaagat agcgttaaag gtcgttttac      480

catcagccgt gatgatgcac gtaataccgt ttatctgcaa atgaatagcc tgaaaccgga      540

agataccgca gtgtattatt gcaacgttaa cgtgggcttt gaatattggg gtcagggcac      600

ccaggttacc gttagcagca aactcgagcg gccgcatcgt gacgcgtcgt ctggtcctgc      660

cggctgccaa gtcctttggg gcgtgaatca gtggaacaca ggtttcacgg cgaatgttac      720

cgtcaagaat acgtcctccg ctcctgttga cggctggacc ttgaccttca gtttcccatc      780

aggacaacaa gtcactcaag cctggtcatc taccgtgacc cagagtggat ctgcggtcac      840

agtacgtaac gctccgtgga acggttcgat tcccgcgggc gggactgctc agttcgggtt      900

taacggaagc cacactggca ctaatgctgc accaactgcc ttctcactta acggcacgcc      960

gtgcaccgta ggcgagctcg aaaacctgta cttccagggt gaacagaaac tgattagcga     1020

agaagatctg tctagagtga ataacaatgg aagcattgtc attaataaca gcattataaa     1080

cgggaatatt acgaatgatg ctgacttaag ttttggtaca gcaaagctgc tctctgctac     1140

agtgaatggt agtcttgtta ataacaaaaa tatcattctt aatcctacaa aagaaagtgc     1200

ggccgctata ggtaatactc ttaccgtgtc aaattatact gggacaccgg gaagtgttat     1260

ttctcttggt ggtgtgcttg aaggagataa ttcacttacg gaccgtctgg tggtgaaagg     1320

taatacctct ggtcaaagtg acatcgttta tgtcaatgaa gatggcagtg gtggtcagac     1380

gagagatggt attaatatta tttctgtaga gggaaattct gatgcagaat tctctctgaa     1440

gaaccgcgta gttgccggag cttatgatta cacactgcag aaaggaaacg agagtgggac     1500

agataataag ggatggtatt taaccagtca tcttcccaca tctgataccc ggcaatacag     1560

accggagaac ggaagttatg ctaccaatat ggcactggct aactcactgt tcctcatgga     1620

tttgaatgag cgtaagcaat tcagggccat gagtgataat acacagcctg agtctgcatc     1680

cgtgtggatg aagatcactg gaggaataag ctctggtaag ctgaatgacg ggcaaaataa     1740

aacaacaacc aatcagttta tcaatcagct cgggggggat atttataaat tccatgctga     1800

acaactgggt gattttacct tagggattat gggaggatac gcgaatgcaa aaggtaaaac     1860

gataaattac acgagcaaca aagctgccag aaacacactg gatggttatt ctgtcggggt     1920

atacggtacg tggtatcaga atggggaaaa tgcaacaggg ctctttgctg aaacttggat     1980

gcaatataac tggtttaatg catcagtgaa aggtgacgga ctggaagaag aaaaatataa     2040

tctgaatggt ttaaccgctt ctgcaggtgg gggatataac ctgaatgtgc acacatggac     2100

atcacctgaa ggaataacag gtgaattctg gttacagcct catttgcagg ctgtctggat     2160

gggggttaca ccggatacac atcaggagga taacggaacg gtggtgcagg gagcagggaa     2220

aaataatatt cagacaaaag caggtattcg tgcatcctgg aaggtgaaaa gcaccctgga     2280

taaggatacc gggcggaggt tccgtccgta tatagaggca aactggatcc ataacactca     2340

tgaatttggt gttaaaatga gtgatgacag ccagttgttg tcaggtagcc gaaatcaggg     2400

agagataaag acaggtattg aaggggtgat tactcaaaac ttgtcagtga atggcggagt     2460

cgcatatcag gcaggaggtc acgggagcaa tgccatctcc ggagcactgg ggataaaata     2520

cagcttctga taatgatcct ggcacgcggc gcgccccttg gtgcgcaaac tattaactgg     2580

cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt     2640

tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg     2700

agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc     2760

ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca     2820

gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc     2880

atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat     2940

cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc     3000

agacccctta ataagatgat cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc     3060

tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag gttctctgag ctaccaactc     3120

tttgaaccga ggtaactggc ttggaggagc gcagtcacca aaacttgtcc tttcagttta     3180

gccttaaccg gcgcatgact tcaagactaa ctcctctaaa tcaattacca gtggctgctg     3240

ccagtggtgc ttttgcatgt ctttccgggt tggactcaag acgatagtta ccggataagg     3300

cgcagcggtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct     3360

acccggaact gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca     3420

ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccgccagg gggaaacgcc     3480

tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga     3540

tgcttgtcag gggggcggag cctatggaaa aacggctttg ccgcggccct ctcacttccc     3600

tgttaagtat cttcctggca tcttccagga aatctccgcc ccgttcgtaa gccatttccg     3660

ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc     3720

tgtatcacat attctgctga cgcaccggtg cagccttttt tctcctgcca catgaagcac     3780

ttcactgaca ccctcatcag tgccaacata gtaagccagt atacactccg ctagcgctga     3840

ggtctgcctc gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc     3900

agccagaaag tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg     3960

attttgaact tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga     4020

tccttcaact cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc     4080

tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac     4140

ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagt     4200

atccgctcat gagattatca aaaaggatct tcacctagat ccttttgtaa gaggttccaa     4260

ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc     4320

aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc     4380

ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa     4440

ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa     4500

gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggagtttcg     4560

tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt     4620

tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg     4680

gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt     4740

ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac     4800

cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg     4860

caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc     4920

cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga     4980

gtggcagggc ggggcgtaat ttttttaagg cgacaccatc gaatggcgca aaacctttcg     5040

cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt     5100

aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt     5160

gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga     5220

gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat     5280

tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa     5340

atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt     5400

cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat     5460

taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc     5520

ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga     5580

agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct     5640

gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata     5700

tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc     5760

cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt     5820

tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt     5880

tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc     5940

gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt     6000

gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt     6060

gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga     6120

ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcaag tgagtggata     6180

accgtattac cgcctttgag tgagctgata ccgggaattc tcactcatta ggcaccccag     6240

gc                                                                    6242


<210>  18
<211>  7157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAIDA-1_GFP-Nanobody_Lipase

<400>  18
tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca       60

cacaggaaag cttcatatga ataaggccta cagtatcatt tggagccact ccagacaggc      120

ctggattgtg gcctcagagt tagccagagg acatggtttt gtccttgcaa aaaatacact      180

gctggtattg gcggttgttt ccacaatcgg aaatgcattt gcagtcgacc accatcacca      240

tcaccatctg gaagcgctgt tccagggtcc gggtaccgtt cagctggttg aaagcggtgg      300

tgcactggtt cagcctggtg gtagcctgcg tctgagctgt gcagcaagcg gttttccggt      360

taatcgttat agcatgcgtt ggtatcgtca ggcaccgggt aaagaacgtg aatgggttgc      420

aggtatgagc agtgccggtg atcgtagcag ctacgaagat agcgttaaag gtcgttttac      480

catcagccgt gatgatgcac gtaataccgt ttatctgcaa atgaatagcc tgaaaccgga      540

agataccgca gtgtattatt gcaacgttaa cgtgggcttt gaatattggg gtcagggcac      600

ccaggttacc gttagcagca aactcgagcg gccgcatcgt gacaagtgct gtcggattat      660

gtttgtgttg ctcggactgt ggtttgtgtt cggcctatcg gtcccgggag ggcggacgga      720

agcggcatcc ctacgcgcca acgacgcacc gattgtgctt ctccacgggt ttaccggctg      780

gggacgagag gaaatgtttg gattcaagta ttggggcggc gtgcgcggcg atatcgaaca      840

gtggctgaac gacaacggtt atcgaacgta tacgctggcg gtcggaccgc tctcgagcaa      900

ctgggaccgg gcgtgtgaag cgtacgctca gcttgtcggc gggacggtcg attacggggc      960

agcccacgcg gcaaagcacg gccacgcgcg gtttggccgc acttatcccg gcctgttgcc     1020

ggaattgaaa aggggtggcc gcatccatat catcgcccac agccaagggg ggcagacggc     1080

ccgcatgctt gtctcgctcc tagagaacgg aagccaagaa gagcgggagt acgccaaggc     1140

gcataacgtg tcgttgtcac cgttgtttga aggtggacat cattttgtgt tgagtgtgac     1200

gaccatcgcc actcctcacg acgggacgac gcttgtcaac atggttgatt tcaccgatcg     1260

cttttttgac ttgcaaaaag cggtgttgga agcggcggct gtcgccagca acgtgccgta     1320

cacgagtcaa gtatacgatt ttaagctcga ccaatgggga ctgcgccgcc agccgggtga     1380

atcgttcgac cattattttg aacggctcaa gcgctcccct gtttggacgt ccacagatac     1440

cgcccgctac gatttatccg tttccggagc tgagaagctc aatcagtggg tgcaagcaag     1500

cccgaatacg tattatttga gtttctctac agaacggacg tatcgcggag cgctcacagg     1560

caaccattat cccgaactcg gaatgaacgc attcagcgcg gtcgtgtgcg ctccgtttct     1620

cggttcgtac cgcaatccga cgctcggcat tgacgaccgt tggttggaga acgatggcat     1680

tgtcaatacg gtttccatga acggtccaaa gcgtggatca agcgatcgga tcgtgccgta     1740

tgacgggacg ttgaaaaaag gggtttggaa cgatatggga acgtacaacg tcgaccattt     1800

ggaaatcatc ggcgttgacc cgaatccgtc atttgatatt cgcgcctttt atttgcggct     1860

tgccgagcag ttggcgagct tgcggcctga gctcgaaaac ctgtacttcc agggtgaaca     1920

gaaactgatt agcgaagaag atctgtctag agtgaataac aatggaagca ttgtcattaa     1980

taacagcatt ataaacggga atattacgaa tgatgctgac ttaagttttg gtacagcaaa     2040

gctgctctct gctacagtga atggtagtct tgttaataac aaaaatatca ttcttaatcc     2100

tacaaaagaa agtgcggccg ctataggtaa tactcttacc gtgtcaaatt atactgggac     2160

accgggaagt gttatttctc ttggtggtgt gcttgaagga gataattcac ttacggaccg     2220

tctggtggtg aaaggtaata cctctggtca aagtgacatc gtttatgtca atgaagatgg     2280

cagtggtggt cagacgagag atggtattaa tattatttct gtagagggaa attctgatgc     2340

agaattctct ctgaagaacc gcgtagttgc cggagcttat gattacacac tgcagaaagg     2400

aaacgagagt gggacagata ataagggatg gtatttaacc agtcatcttc ccacatctga     2460

tacccggcaa tacagaccgg agaacggaag ttatgctacc aatatggcac tggctaactc     2520

actgttcctc atggatttga atgagcgtaa gcaattcagg gccatgagtg ataatacaca     2580

gcctgagtct gcatccgtgt ggatgaagat cactggagga ataagctctg gtaagctgaa     2640

tgacgggcaa aataaaacaa caaccaatca gtttatcaat cagctcgggg gggatattta     2700

taaattccat gctgaacaac tgggtgattt taccttaggg attatgggag gatacgcgaa     2760

tgcaaaaggt aaaacgataa attacacgag caacaaagct gccagaaaca cactggatgg     2820

ttattctgtc ggggtatacg gtacgtggta tcagaatggg gaaaatgcaa cagggctctt     2880

tgctgaaact tggatgcaat ataactggtt taatgcatca gtgaaaggtg acggactgga     2940

agaagaaaaa tataatctga atggtttaac cgcttctgca ggtgggggat ataacctgaa     3000

tgtgcacaca tggacatcac ctgaaggaat aacaggtgaa ttctggttac agcctcattt     3060

gcaggctgtc tggatggggg ttacaccgga tacacatcag gaggataacg gaacggtggt     3120

gcagggagca gggaaaaata atattcagac aaaagcaggt attcgtgcat cctggaaggt     3180

gaaaagcacc ctggataagg ataccgggcg gaggttccgt ccgtatatag aggcaaactg     3240

gatccataac actcatgaat ttggtgttaa aatgagtgat gacagccagt tgttgtcagg     3300

tagccgaaat cagggagaga taaagacagg tattgaaggg gtgattactc aaaacttgtc     3360

agtgaatggc ggagtcgcat atcaggcagg aggtcacggg agcaatgcca tctccggagc     3420

actggggata aaatacagct tctgataatg atcctggcac gcggcgcgcc ccttggtgcg     3480

caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat     3540

ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat     3600

tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc     3660

agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga     3720

tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc     3780

agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag     3840

gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc     3900

gttccactga gcgtcagacc ccttaataag atgatcttct tgagatcgtt ttggtctgcg     3960

cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct     4020

ctgagctacc aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact     4080

tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct ctaaatcaat     4140

taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac tcaagacgat     4200

agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct     4260

tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat     4320

aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg     4380

ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag     4440

cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg     4500

gccctctcac ttccctgtta agtatcttcc tggcatcttc caggaaatct ccgccccgtt     4560

cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg     4620

aagcggaata tatcctgtat cacatattct gctgacgcac cggtgcagcc ttttttctcc     4680

tgccacatga agcacttcac tgacaccctc atcagtgcca acatagtaag ccagtataca     4740

ctccgctagc gctgaggtct gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga     4800

atcgccccat catccagcca gaaagtgagg gagccacggt tgatgagagc tttgttgtag     4860

gtggaccagt tggtgatttt gaacttttgc tttgccacgg aacggtctgc gttgtcggga     4920

agatgcgtga tctgatcctt caactcagca aaagttcgat ttattcaaca aagccacgtt     4980

gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata     5040

aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa     5100

acgtcttgct cgagtatccg ctcatgagat tatcaaaaag gatcttcacc tagatccttt     5160

tgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga     5220

gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac     5280

caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc     5340

tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa     5400

gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc     5460

tcatccggag tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca     5520

cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata     5580

ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga     5640

aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc     5700

ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc     5760

cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat     5820

tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca     5880

acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcgaca ccatcgaatg     5940

gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt     6000

gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac     6060

cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga     6120

agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa     6180

acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat     6240

tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt     6300

agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt     6360

cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc     6420

ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat     6480

tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca     6540

ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc     6600

tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga     6660

ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc     6720

cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga     6780

gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag     6840

ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac     6900

cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt     6960

gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc     7020

ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg     7080

gcaagtgagt ggataaccgt attaccgcct ttgagtgagc tgataccggg aattctcact     7140

cattaggcac cccaggc                                                    7157


<210>  19
<211>  4341
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pET-9a

<400>  19
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata      240

tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg      300

ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac      360

cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga      420

cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact      480

cagcttcctt tcgggctttg ttagcagccg gatccgcgac ccatttgctg tccaccagtc      540

atgctagcca tatgtatatc tccttcttaa agttaaacaa aattatttct agagggaaac      600

cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct      660

acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata      720

tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt      780

tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc      840

atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc      900

taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag      960

tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct     1020

ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc     1080

gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg     1140

ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca     1200

ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag     1260

gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt     1320

tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc     1380

tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg     1440

ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg     1500

tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag     1560

ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg     1620

gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc     1680

cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat     1740

cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa     1800

tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg     1860

agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc     1920

agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg     1980

aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc     2040

ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc     2100

atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat     2160

gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa     2220

catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga     2280

cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg     2340

cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga     2400

gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc     2460

agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt     2520

gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc     2580

ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt     2640

cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact     2700

caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag     2760

caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata     2820

ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc     2880

cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg     2940

ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc     3000

tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg     3060

gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc     3120

ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga     3180

ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg     3240

gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa     3300

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg     3360

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt     3420

ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaaca     3480

ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg     3540

gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat     3600

aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag     3660

cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca     3720

gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat     3780

tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca     3840

ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg     3900

ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta     3960

tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt     4020

gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg     4080

ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt     4140

gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac     4200

caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg     4260

ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg     4320

ctcgatgagt ttttctaaga a                                               4341


<210>  20
<211>  4981
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGEX-6P-1_NoGST

<400>  20
acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg       60

gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt      120

tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc      180

tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca      240

cacaggaaac agtattctcc cctatactag gttattggaa aattaagggc cttgtgcaac      300

ccactcgact tcttttggaa tatcttgaag aaaaatatga agagcatttg tatgagcgcg      360

atgaaggtga taaatggcga aacaaaaagt ttgaattggg tttggagttt cccaatcttc      420

cttattatat tgatggtgat gttaaattaa cacagtctat ggccatcata cgttatatag      480

ctgacaagca caacatgttg ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg      540

aaggagcggt tttggatatt agatacggtg tttcgagaat tgcatatagt aaagactttg      600

aaactctcaa agttgatttt cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc      660

gtttatgtca taaaacatat ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt      720

atgacgctct tgatgttgtt ttatacatgg acccaatgtg cctggatgcg ttcccaaaat      780

tagtttgttt taaaaaacgt attgaagcta tcccacaaat tgataagtac ttgaaatcca      840

gcaagtatat agcatggcct ttgcagggct ggcaagccac gtttggtggt ggcgaccatc      900

ctccaaaatc ggatctggaa gttctgttcc aggggcccct gggatccccg gaattcccgg      960

gtcgactcga gcggccgcat cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg     1020

acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg     1080

atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg     1140

cagccatgac ccagtcacgt agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc     1200

tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag     1260

gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt     1320

caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa     1380

ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt     1440

gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt     1500

tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt     1560

ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg     1620

tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga     1680

atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa     1740

gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga     1800

caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa     1860

ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca     1920

ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta     1980

ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac     2040

ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc     2100

gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag     2160

ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga     2220

taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt     2280

agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata     2340

atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag     2400

aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa     2460

caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt     2520

ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc     2580

cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa     2640

tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa     2700

gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc     2760

ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa     2820

gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa     2880

caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg     2940

ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc     3000

tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg     3060

ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg     3120

agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg     3180

aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc     3240

gcataaattc cgacaccatc gaatggtgca aaacctttcg cggtatggca tgatagcgcc     3300

cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag     3360

agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt     3420

ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc     3480

gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc     3540

tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg     3600

gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg     3660

tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc     3720

aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct     3780

ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg     3840

tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt     3900

ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc     3960

agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc     4020

aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc     4080

tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag     4140

tgggatacga cgataccgaa gacagctcat gttatatccc gccgtcaacc accatcaaac     4200

aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc     4260

aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg     4320

cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac     4380

gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc     4440

actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt     4500

gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgg attcactggc     4560

cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc     4620

agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc     4680

ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc     4740

ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc     4800

aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtaacct atcccattac     4860

ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa     4920

tgttgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttggaat     4980

t                                                                     4981


<210>  21
<211>  7321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGEX-6P-1_NoGST_Brk-CBM

<400>  21
acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg       60

gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt      120

tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc      180

tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca      240

cacaggaaac agtattctcc cctatactag gttattggaa aattaagggc cttgtgcaac      300

ccactcgact tcttttggaa tatcttgaag aaaaatatga agagcatttg tatgagcgcg      360

atgaaggtga taaatggcga aacaaaaagt ttgaattggg tttggagttt cccaatcttc      420

cttattatat tgatggtgat gttaaattaa cacagtctat ggccatcata cgttatatag      480

ctgacaagca caacatgttg ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg      540

aaggagcggt tttggatatt agatacggtg tttcgagaat tgcatatagt aaagactttg      600

aaactctcaa agttgatttt cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc      660

gtttatgtca taaaacatat ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt      720

atgacgctct tgatgttgtt ttatacatgg acccaatgtg cctggatgcg ttcccaaaat      780

tagtttgttt taaaaaacgt attgaagcta tcccacaaat tgataagtac ttgaaatcca      840

gcaagtatat agcatggcct ttgcagggct ggcaagccac gtttggtggt ggcgaccatc      900

ctccaaaatc ggatctggaa gttctgttcc aggggcccct gggatccatg tatctggatc      960

gctttcgcca gtgcccgagc agcctgcaga ttccgcgcag cgcgtggcgc ctgcatgcgc     1020

tggcggcggc gctggcgctg gcgggcatgg cgcgcctggc gccggcggcg gcgcaggcgc     1080

cgcagccgcc ggtggcgggc gcgccgcatg cgcaggatgc gggccaccat caccatcacc     1140

atgttcagct ggttgaaagc ggtggtgcac tggttcagcc tggtggtagc ctgcgtctga     1200

gctgtgcagc aagcggtttt ccggttaatc gttatagcat gcgttggtat cgtcaggcac     1260

cgggtaaaga acgtgaatgg gttgcaggta tgagcagtgc cggtgatcgt agcagctacg     1320

aagatagcgt taaaggtcgt tttaccatca gccgtgatga tgcacgtaat accgtttatc     1380

tgcaaatgaa tagcctgaaa ccggaagata ccgcagtgta ttattgcaac gttaacgtgg     1440

gctttgaata ttggggtcag ggcacccagg ttaccgttag cagcaaactc gagcggccgc     1500

atcgtgacgc gtcgtctggt cctgccggct gccaagtcct ttggggcgtg aatcagtgga     1560

acacaggttt cacggcgaat gttaccgtca agaatacgtc ctccgctcct gttgacggct     1620

ggaccttgac cttcagtttc ccatcaggac aacaagtcac tcaagcctgg tcatctaccg     1680

tgacccagag tggatctgcg gtcacagtac gtaacgctcc gtggaacggt tcgattcccg     1740

cgggcgggac tgctcagttc gggtttaacg gaagccacac tggcactaat gctgcaccaa     1800

ctgccttctc acttaacggc acgccgtgca ccgtaggcga acagaaactg attagcgaag     1860

aagatctgga aaacctgtac ttccagggtg cgggcattag cctgagcgtg gcgagcggcg     1920

cggcgtggca tggcgcgacc caggtgctgc agagcgcgac cctgggcaaa ggcggcacct     1980

gggtggtgaa cgcggatagc cgcgtgcagg atatgagcat gcgcggcggc cgcgtggaat     2040

ttcaggcgcc ggcgccggaa gcgagctata aaaccctgac cctgcagacc ctggatggca     2100

acggcgtgtt tgtgctgaac accaacgtgg cggcgggcca gaacgatcag ctgcgcgtga     2160

ccggccgcgc ggatggccag catcgcgtgc tggtgcgcaa cgcgggcggc gaagcggata     2220

gccgcggcgc gcgcctgggc ctggtgcata cccagggcca gggcaacgcg acctttcgcc     2280

tggcgaacgt gggcaaagcg gtggatctgg gcacctggcg ctatagcctg gcggaagatc     2340

cgaaaaccca tgtgtggagc ctgcagcgcg cgggccaggc gctgagcggc gcggcgaacg     2400

cggcggtgaa cgcggcggat ctgagcagca ttgcgctggc ggaaagcaac gcgctggata     2460

aacgcctggg cgaactgcgc ctgcgcgcgg atgcgggcgg cccgtgggcg cgcaccttta     2520

gcgaacgcca gcagattagc aaccgccatg cgcgcgcgta tgatcagacc gtgagcggcc     2580

tggaaattgg cctggatcgc ggctggagcg cgagcggcgg ccgctggtat gcgggcggcc     2640

tgctgggcta tacctatgcg gatcgcacct atccgggcga tggcggcggc aaagtgaaag     2700

gcctgcatgt gggcggctat gcggcgtatg tgggcgatgg cggctattat ctggataccg     2760

tgctgcgcct gggccgctat gatcagcagt ataacattgc gggcaccgat ggcggccgcg     2820

tgaccgcgga ttatcgcacc agcggcgcgg cgtggagcct ggaaggcggc cgccgctttg     2880

aactgccgaa cgattggttt gcggaaccgc aggcggaagt gatgctgtgg cgcaccagcg     2940

gcaaacgcta tcgcgcgagc aacggcctgc gcgtgaaagt ggatgcgaac accgcgaccc     3000

tgggccgcct gggcctgcgc tttggccgcc gcattgcgct ggcgggcggc aacattgtgc     3060

agccgtatgc gcgcctgggc tggacccagg aatttaaaag caccggcgat gtgcgcacca     3120

acggcattgg ccatgcgggc gcgggccgcc atggccgcgt ggaactgggc gcgggcgtgg     3180

atgcggcgct gggcaaaggc cataacctgt atgcgagcta tgaatatgcg gcgggcgatc     3240

gcattaacat tccgtggagc tttcatgcgg gctatcgcta tagcttttga gaattcccgg     3300

gtcgactcga gcggccgcat cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg     3360

acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg     3420

atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg     3480

cagccatgac ccagtcacgt agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc     3540

tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag     3600

gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt     3660

caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa     3720

ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt     3780

gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt     3840

tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt     3900

ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg     3960

tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga     4020

atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa     4080

gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga     4140

caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa     4200

ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca     4260

ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta     4320

ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac     4380

ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc     4440

gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag     4500

ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga     4560

taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt     4620

agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata     4680

atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag     4740

aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa     4800

caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt     4860

ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc     4920

cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa     4980

tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa     5040

gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc     5100

ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa     5160

gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa     5220

caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg     5280

ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc     5340

tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg     5400

ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg     5460

agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg     5520

aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc     5580

gcataaattc cgacaccatc gaatggtgca aaacctttcg cggtatggca tgatagcgcc     5640

cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag     5700

agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt     5760

ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc     5820

gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc     5880

tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg     5940

gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg     6000

tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc     6060

aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct     6120

ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg     6180

tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt     6240

ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc     6300

agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc     6360

aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc     6420

tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag     6480

tgggatacga cgataccgaa gacagctcat gttatatccc gccgtcaacc accatcaaac     6540

aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc     6600

aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg     6660

cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac     6720

gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc     6780

actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt     6840

gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgg attcactggc     6900

cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc     6960

agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc     7020

ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc     7080

ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc     7140

aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtaacct atcccattac     7200

ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa     7260

tgttgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttggaat     7320

t                                                                     7321


<210>  22
<211>  8236
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGEX-6P-1_NoGST_Brk-Lipase

<400>  22
acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg       60

gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt      120

tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc      180

tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca      240

cacaggaaac agtattctcc cctatactag gttattggaa aattaagggc cttgtgcaac      300

ccactcgact tcttttggaa tatcttgaag aaaaatatga agagcatttg tatgagcgcg      360

atgaaggtga taaatggcga aacaaaaagt ttgaattggg tttggagttt cccaatcttc      420

cttattatat tgatggtgat gttaaattaa cacagtctat ggccatcata cgttatatag      480

ctgacaagca caacatgttg ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg      540

aaggagcggt tttggatatt agatacggtg tttcgagaat tgcatatagt aaagactttg      600

aaactctcaa agttgatttt cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc      660

gtttatgtca taaaacatat ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt      720

atgacgctct tgatgttgtt ttatacatgg acccaatgtg cctggatgcg ttcccaaaat      780

tagtttgttt taaaaaacgt attgaagcta tcccacaaat tgataagtac ttgaaatcca      840

gcaagtatat agcatggcct ttgcagggct ggcaagccac gtttggtggt ggcgaccatc      900

ctccaaaatc ggatctggaa gttctgttcc aggggcccct gggatccatg tatctggatc      960

gctttcgcca gtgcccgagc agcctgcaga ttccgcgcag cgcgtggcgc ctgcatgcgc     1020

tggcggcggc gctggcgctg gcgggcatgg cgcgcctggc gccggcggcg gcgcaggcgc     1080

cgcagccgcc ggtggcgggc gcgccgcatg cgcaggatgc gggccaccat caccatcacc     1140

atgttcagct ggttgaaagc ggtggtgcac tggttcagcc tggtggtagc ctgcgtctga     1200

gctgtgcagc aagcggtttt ccggttaatc gttatagcat gcgttggtat cgtcaggcac     1260

cgggtaaaga acgtgaatgg gttgcaggta tgagcagtgc cggtgatcgt agcagctacg     1320

aagatagcgt taaaggtcgt tttaccatca gccgtgatga tgcacgtaat accgtttatc     1380

tgcaaatgaa tagcctgaaa ccggaagata ccgcagtgta ttattgcaac gttaacgtgg     1440

gctttgaata ttggggtcag ggcacccagg ttaccgttag cagcaaactc gagcggccgc     1500

atcgtgacaa gtgctgtcgg attatgtttg tgttgctcgg actgtggttt gtgttcggcc     1560

tatcggtccc gggagggcgg acggaagcgg catccctacg cgccaacgac gcaccgattg     1620

tgcttctcca cgggtttacc ggctggggac gagaggaaat gtttggattc aagtattggg     1680

gcggcgtgcg cggcgatatc gaacagtggc tgaacgacaa cggttatcga acgtatacgc     1740

tggcggtcgg accgctctcg agcaactggg accgggcgtg tgaagcgtac gctcagcttg     1800

tcggcgggac ggtcgattac ggggcagccc acgcggcaaa gcacggccac gcgcggtttg     1860

gccgcactta tcccggcctg ttgccggaat tgaaaagggg tggccgcatc catatcatcg     1920

cccacagcca aggggggcag acggcccgca tgcttgtctc gctcctagag aacggaagcc     1980

aagaagagcg ggagtacgcc aaggcgcata acgtgtcgtt gtcaccgttg tttgaaggtg     2040

gacatcattt tgtgttgagt gtgacgacca tcgccactcc tcacgacggg acgacgcttg     2100

tcaacatggt tgatttcacc gatcgctttt ttgacttgca aaaagcggtg ttggaagcgg     2160

cggctgtcgc cagcaacgtg ccgtacacga gtcaagtata cgattttaag ctcgaccaat     2220

ggggactgcg ccgccagccg ggtgaatcgt tcgaccatta ttttgaacgg ctcaagcgct     2280

cccctgtttg gacgtccaca gataccgccc gctacgattt atccgtttcc ggagctgaga     2340

agctcaatca gtgggtgcaa gcaagcccga atacgtatta tttgagtttc tctacagaac     2400

ggacgtatcg cggagcgctc acaggcaacc attatcccga actcggaatg aacgcattca     2460

gcgcggtcgt gtgcgctccg tttctcggtt cgtaccgcaa tccgacgctc ggcattgacg     2520

accgttggtt ggagaacgat ggcattgtca atacggtttc catgaacggt ccaaagcgtg     2580

gatcaagcga tcggatcgtg ccgtatgacg ggacgttgaa aaaaggggtt tggaacgata     2640

tgggaacgta caacgtcgac catttggaaa tcatcggcgt tgacccgaat ccgtcatttg     2700

atattcgcgc cttttatttg cggcttgccg agcagttggc gagcttgcgg cctgaacaga     2760

aactgattag cgaagaagat ctggaaaacc tgtacttcca gggtgcgggc attagcctga     2820

gcgtggcgag cggcgcggcg tggcatggcg cgacccaggt gctgcagagc gcgaccctgg     2880

gcaaaggcgg cacctgggtg gtgaacgcgg atagccgcgt gcaggatatg agcatgcgcg     2940

gcggccgcgt ggaatttcag gcgccggcgc cggaagcgag ctataaaacc ctgaccctgc     3000

agaccctgga tggcaacggc gtgtttgtgc tgaacaccaa cgtggcggcg ggccagaacg     3060

atcagctgcg cgtgaccggc cgcgcggatg gccagcatcg cgtgctggtg cgcaacgcgg     3120

gcggcgaagc ggatagccgc ggcgcgcgcc tgggcctggt gcatacccag ggccagggca     3180

acgcgacctt tcgcctggcg aacgtgggca aagcggtgga tctgggcacc tggcgctata     3240

gcctggcgga agatccgaaa acccatgtgt ggagcctgca gcgcgcgggc caggcgctga     3300

gcggcgcggc gaacgcggcg gtgaacgcgg cggatctgag cagcattgcg ctggcggaaa     3360

gcaacgcgct ggataaacgc ctgggcgaac tgcgcctgcg cgcggatgcg ggcggcccgt     3420

gggcgcgcac ctttagcgaa cgccagcaga ttagcaaccg ccatgcgcgc gcgtatgatc     3480

agaccgtgag cggcctggaa attggcctgg atcgcggctg gagcgcgagc ggcggccgct     3540

ggtatgcggg cggcctgctg ggctatacct atgcggatcg cacctatccg ggcgatggcg     3600

gcggcaaagt gaaaggcctg catgtgggcg gctatgcggc gtatgtgggc gatggcggct     3660

attatctgga taccgtgctg cgcctgggcc gctatgatca gcagtataac attgcgggca     3720

ccgatggcgg ccgcgtgacc gcggattatc gcaccagcgg cgcggcgtgg agcctggaag     3780

gcggccgccg ctttgaactg ccgaacgatt ggtttgcgga accgcaggcg gaagtgatgc     3840

tgtggcgcac cagcggcaaa cgctatcgcg cgagcaacgg cctgcgcgtg aaagtggatg     3900

cgaacaccgc gaccctgggc cgcctgggcc tgcgctttgg ccgccgcatt gcgctggcgg     3960

gcggcaacat tgtgcagccg tatgcgcgcc tgggctggac ccaggaattt aaaagcaccg     4020

gcgatgtgcg caccaacggc attggccatg cgggcgcggg ccgccatggc cgcgtggaac     4080

tgggcgcggg cgtggatgcg gcgctgggca aaggccataa cctgtatgcg agctatgaat     4140

atgcggcggg cgatcgcatt aacattccgt ggagctttca tgcgggctat cgctatagct     4200

tttgagaatt cccgggtcga ctcgagcggc cgcatcgtga ctgactgacg atctgcctcg     4260

cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag     4320

cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg     4380

gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tataattctt     4440

gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg     4500

tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat     4560

ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc     4620

aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct     4680

tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag     4740

atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta     4800

agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc     4860

tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca     4920

tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg     4980

atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg     5040

ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca     5100

tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa     5160

acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa     5220

ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata     5280

aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat     5340

ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc     5400

cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata     5460

gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt     5520

actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga     5580

agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag     5640

cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa     5700

tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag     5760

agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg     5820

tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat     5880

acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta     5940

ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg     6000

gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc     6060

gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa     6120

gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc     6180

tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt     6240

caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct     6300

tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc     6360

gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg     6420

agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt     6480

gcggtatttc acaccgcata aattccgaca ccatcgaatg gtgcaaaacc tttcgcggta     6540

tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt     6600

tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc     6660

aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga     6720

attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg     6780

ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc     6840

gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag     6900

cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact     6960

atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt     7020

tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg     7080

gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag     7140

cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca     7200

ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt     7260

ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca     7320

acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg     7380

cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt     7440

caaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc     7500

aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa     7560

gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat     7620

taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt     7680

aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt     7740

atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat     7800

tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca     7860

acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg     7920

caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt     7980

tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac     8040

tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt     8100

aacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta     8160

ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt     8220

tgatggcgtt ggaatt                                                     8236


<210>  23
<211>  6283
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGEX-6P-1_NoGST_InaK-CBM

<400>  23
acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg       60

gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt      120

tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc      180

tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca      240

cacaggaaac agtattctcc cctatactag gttattggaa aattaagggc cttgtgcaac      300

ccactcgact tcttttggaa tatcttgaag aaaaatatga agagcatttg tatgagcgcg      360

atgaaggtga taaatggcga aacaaaaagt ttgaattggg tttggagttt cccaatcttc      420

cttattatat tgatggtgat gttaaattaa cacagtctat ggccatcata cgttatatag      480

ctgacaagca caacatgttg ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg      540

aaggagcggt tttggatatt agatacggtg tttcgagaat tgcatatagt aaagactttg      600

aaactctcaa agttgatttt cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc      660

gtttatgtca taaaacatat ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt      720

atgacgctct tgatgttgtt ttatacatgg acccaatgtg cctggatgcg ttcccaaaat      780

tagtttgttt taaaaaacgt attgaagcta tcccacaaat tgataagtac ttgaaatcca      840

gcaagtatat agcatggcct ttgcagggct ggcaagccac gtttggtggt ggcgaccatc      900

ctccaaaatc ggatctggaa gttctgttcc aggggcccct gggatccatg gtcttagaca      960

aggcgctagt tctacgtacc tgcgctaata atatggccga tcactgcggc ttgatttggc     1020

ctgcctcagg gaccgtcgag tcaaggtatt ggcaatctac acgtcgtcac gagaacggac     1080

tggtaggtct tctttgggga gcaggaactt ctgctttctt gtcagtccat gcagacgccc     1140

gctggatcgt gtgcgaagtg gctgttgccg atattatctc cctagaggag cccggaatgg     1200

ttaaatttcc tcgggccgaa gtggtgcatg tgggcgatcg aatcagcgct tctcatttta     1260

tttcggcgcg gcaggcagat cccgcgagta cgagtacttc aacgtcgaca agtactctta     1320

ctcccatgcc cacggcaatc cccaccccta tgccagcggt ggcgtcagtg acgttaccgg     1380

tggctgagca agcgcggcat gaggtgtttg atgtagctag tgtgagcgcc gcggctgctc     1440

ccgtgaacac tttacctgtc acgacacccc aaaacctcca gacggaaaac ctgtacttcc     1500

agggtgcgtc gtctggtcct gccggctgcc aagtcctttg gggcgtgaat cagtggaaca     1560

caggtttcac ggcgaatgtt accgtcaaga atacgtcctc cgctcctgtt gacggctgga     1620

ccttgacctt cagtttccca tcaggacaac aagtcactca agcctggtca tctaccgtga     1680

cccagagtgg atctgcggtc acagtacgta acgctccgtg gaacggttcg attcccgcgg     1740

gcgggactgc tcagttcggg tttaacggaa gccacactgg cactaatgct gcaccaactg     1800

ccttctcact taacggcacg ccgtgcaccg taggccacca tcaccatcac catgttcagc     1860

tggttgaaag cggtggtgca ctggttcagc ctggtggtag cctgcgtctg agctgtgcag     1920

caagcggttt tccggttaat cgttatagca tgcgttggta tcgtcaggca ccgggtaaag     1980

aacgtgaatg ggttgcaggt atgagcagtg ccggtgatcg tagcagctac gaagatagcg     2040

ttaaaggtcg ttttaccatc agccgtgatg atgcacgtaa taccgtttat ctgcaaatga     2100

atagcctgaa accggaagat accgcagtgt attattgcaa cgttaacgtg ggctttgaat     2160

attggggtca gggcacccag gttaccgtta gcagcaaact cgagcggccg catcgtgacg     2220

aacagaaact gattagcgaa gaagatctgt gagaattccc gggtcgactc gagcggccgc     2280

atcgtgactg actgacgatc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac     2340

acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag     2400

cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac     2460

gtagcgatag cggagtgtat aattcttgaa gacgaaaggg cctcgtgata cgcctatttt     2520

tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa     2580

atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca     2640

tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc     2700

aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc     2760

acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt     2820

acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt     2880

ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg     2940

ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact     3000

caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg     3060

ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga     3120

aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg     3180

aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa     3240

tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac     3300

aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc     3360

cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca     3420

ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga     3480

gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta     3540

agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc     3600

atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc     3660

cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt     3720

cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac     3780

cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct     3840

tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact     3900

tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg     3960

ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata     4020

aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga     4080

cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag     4140

ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg     4200

agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac     4260

ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca     4320

acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg     4380

cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc     4440

gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga     4500

tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataaat tccgacacca     4560

tcgaatggtg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca     4620

gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt     4680

atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa     4740

aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg     4800

cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt     4860

cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt     4920

cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc     4980

aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg     5040

aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca     5100

acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat     5160

tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc     5220

gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg     5280

aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca     5340

tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca     5400

ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg     5460

aagacagctc atgttatatc ccgccgtcaa ccaccatcaa acaggatttt cgcctgctgg     5520

ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc     5580

agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg     5640

cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg     5700

aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag     5760

gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt     5820

cacacaggaa acagctatga ccatgattac ggattcactg gccgtcgttt tacaacgtcg     5880

tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc     5940

cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct     6000

gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct     6060

ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg     6120

ttacgatgcg cccatctaca ccaacgtaac ctatcccatt acggtcaatc cgccgtttgt     6180

tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct     6240

acaggaaggc cagacgcgaa ttatttttga tggcgttgga att                       6283


<210>  24
<211>  7198
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGEX-6P-1_NoGST_InaK-Lipase

<400>  24
acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg       60

gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt      120

tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc      180

tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca      240

cacaggaaac agtattctcc cctatactag gttattggaa aattaagggc cttgtgcaac      300

ccactcgact tcttttggaa tatcttgaag aaaaatatga agagcatttg tatgagcgcg      360

atgaaggtga taaatggcga aacaaaaagt ttgaattggg tttggagttt cccaatcttc      420

cttattatat tgatggtgat gttaaattaa cacagtctat ggccatcata cgttatatag      480

ctgacaagca caacatgttg ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg      540

aaggagcggt tttggatatt agatacggtg tttcgagaat tgcatatagt aaagactttg      600

aaactctcaa agttgatttt cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc      660

gtttatgtca taaaacatat ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt      720

atgacgctct tgatgttgtt ttatacatgg acccaatgtg cctggatgcg ttcccaaaat      780

tagtttgttt taaaaaacgt attgaagcta tcccacaaat tgataagtac ttgaaatcca      840

gcaagtatat agcatggcct ttgcagggct ggcaagccac gtttggtggt ggcgaccatc      900

ctccaaaatc ggatctggaa gttctgttcc aggggcccct gggatccatg gtcttagaca      960

aggcgctagt tctacgtacc tgcgctaata atatggccga tcactgcggc ttgatttggc     1020

ctgcctcagg gaccgtcgag tcaaggtatt ggcaatctac acgtcgtcac gagaacggac     1080

tggtaggtct tctttgggga gcaggaactt ctgctttctt gtcagtccat gcagacgccc     1140

gctggatcgt gtgcgaagtg gctgttgccg atattatctc cctagaggag cccggaatgg     1200

ttaaatttcc tcgggccgaa gtggtgcatg tgggcgatcg aatcagcgct tctcatttta     1260

tttcggcgcg gcaggcagat cccgcgagta cgagtacttc aacgtcgaca agtactctta     1320

ctcccatgcc cacggcaatc cccaccccta tgccagcggt ggcgtcagtg acgttaccgg     1380

tggctgagca agcgcggcat gaggtgtttg atgtagctag tgtgagcgcc gcggctgctc     1440

ccgtgaacac tttacctgtc acgacacccc aaaacctcca gacggaaaac ctgtacttcc     1500

agggtaagtg ctgtcggatt atgtttgtgt tgctcggact gtggtttgtg ttcggcctat     1560

cggtcccggg agggcggacg gaagcggcat ccctacgcgc caacgacgca ccgattgtgc     1620

ttctccacgg gtttaccggc tggggacgag aggaaatgtt tggattcaag tattggggcg     1680

gcgtgcgcgg cgatatcgaa cagtggctga acgacaacgg ttatcgaacg tatacgctgg     1740

cggtcggacc gctctcgagc aactgggacc gggcgtgtga agcgtacgct cagcttgtcg     1800

gcgggacggt cgattacggg gcagcccacg cggcaaagca cggccacgcg cggtttggcc     1860

gcacttatcc cggcctgttg ccggaattga aaaggggtgg ccgcatccat atcatcgccc     1920

acagccaagg ggggcagacg gcccgcatgc ttgtctcgct cctagagaac ggaagccaag     1980

aagagcggga gtacgccaag gcgcataacg tgtcgttgtc accgttgttt gaaggtggac     2040

atcattttgt gttgagtgtg acgaccatcg ccactcctca cgacgggacg acgcttgtca     2100

acatggttga tttcaccgat cgcttttttg acttgcaaaa agcggtgttg gaagcggcgg     2160

ctgtcgccag caacgtgccg tacacgagtc aagtatacga ttttaagctc gaccaatggg     2220

gactgcgccg ccagccgggt gaatcgttcg accattattt tgaacggctc aagcgctccc     2280

ctgtttggac gtccacagat accgcccgct acgatttatc cgtttccgga gctgagaagc     2340

tcaatcagtg ggtgcaagca agcccgaata cgtattattt gagtttctct acagaacgga     2400

cgtatcgcgg agcgctcaca ggcaaccatt atcccgaact cggaatgaac gcattcagcg     2460

cggtcgtgtg cgctccgttt ctcggttcgt accgcaatcc gacgctcggc attgacgacc     2520

gttggttgga gaacgatggc attgtcaata cggtttccat gaacggtcca aagcgtggat     2580

caagcgatcg gatcgtgccg tatgacggga cgttgaaaaa aggggtttgg aacgatatgg     2640

gaacgtacaa cgtcgaccat ttggaaatca tcggcgttga cccgaatccg tcatttgata     2700

ttcgcgcctt ttatttgcgg cttgccgagc agttggcgag cttgcggcct caccatcacc     2760

atcaccatgt tcagctggtt gaaagcggtg gtgcactggt tcagcctggt ggtagcctgc     2820

gtctgagctg tgcagcaagc ggttttccgg ttaatcgtta tagcatgcgt tggtatcgtc     2880

aggcaccggg taaagaacgt gaatgggttg caggtatgag cagtgccggt gatcgtagca     2940

gctacgaaga tagcgttaaa ggtcgtttta ccatcagccg tgatgatgca cgtaataccg     3000

tttatctgca aatgaatagc ctgaaaccgg aagataccgc agtgtattat tgcaacgtta     3060

acgtgggctt tgaatattgg ggtcagggca cccaggttac cgttagcagc aaactcgagc     3120

ggccgcatcg tgacgaacag aaactgatta gcgaagaaga tctgtgagaa ttcccgggtc     3180

gactcgagcg gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg     3240

gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg     3300

ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag     3360

ccatgaccca gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg     3420

tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg     3480

gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa     3540

atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga     3600

agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc     3660

ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg     3720

gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc     3780

gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat     3840

tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg     3900

acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag     3960

aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa     4020

cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc     4080

gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca     4140

cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc     4200

tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc     4260

tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg     4320

ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta     4380

tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag     4440

gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga     4500

ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc     4560

tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa     4620

agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa     4680

aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc     4740

cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt     4800

agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc     4860

tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac     4920

gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca     4980

gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg     5040

ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag     5100

gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt     5160

ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat     5220

ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc     5280

acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt     5340

gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag     5400

cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca     5460

taaattccga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg     5520

aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt     5580

atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg     5640

cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg     5700

tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg     5760

ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg     5820

ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc     5880

acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg     5940

atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg     6000

accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg     6060

agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg     6120

tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc     6180

cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa     6240

tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg     6300

gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg     6360

gatacgacga taccgaagac agctcatgtt atatcccgcc gtcaaccacc atcaaacagg     6420

attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg     6480

cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc     6540

ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac     6600

aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact     6660

cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg     6720

agcggataac aatttcacac aggaaacagc tatgaccatg attacggatt cactggccgt     6780

cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc     6840

acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca     6900

acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt     6960

gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa     7020

ctggcagatg cacggttacg atgcgcccat ctacaccaac gtaacctatc ccattacggt     7080

caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt     7140

tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttggaatt       7198


<210>  25
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer F2 minCKO

<400>  25
aacaacaata atgcgtgcca t                                                 21


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer R2 minCKO

<400>  26
gcgctggcga tgattaatag                                                   20


<210>  27
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer F9 minCKO

<400>  27
agtaacaaca ataatgcgtg cc                                                22


<210>  28
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer R9 minCKO

<400>  28
cgcgctggcg atgatt                                                       16


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer F7 minDKO

<400>  29
ttccgcgaga gaaagaaatc g                                                 21


<210>  30
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer R7 minDKO

<400>  30
gaccgttcaa ccgttaaatt gat                                               23


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer F10 minDKO

<400>  31
ctgtgttttt cttccgcgag                                                   20


<210>  32
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer R10 minDKO

<400>  32
tcaaccgtta aattgatccc ttttt                                             25


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer F6 minCDKO

<400>  33
tccgcgagag aaagaaatcg                                                   20


<210>  34
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer R6 minCDKO

<400>  34
cgcgctggcg atgatta                                                      17


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer F9 minCDKO

<400>  35
ctgtgttttt cttccgcgag                                                   20


<210>  36
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer R9 minCDKO

<400>  36
cgcgctggcg atgatt                                                       16


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer 3'minCKO_1

<400>  37
ggccggataa aacttgtgct                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer 3'minCKO_2

<400>  38
agtcttcgga acatcatcgc                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer 5'minCKO_1

<400>  39
ccctttgccc gaagtaacaa                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer 5'minCKO_2

<400>  40
acggtgaaaa cctggcctat                                                   20


<210>  41
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer minC_check_4_1

<400>  41
tcaatttaac ggttgaacgg tca                                               23


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer minC_check_4_2

<400>  42
atgtcaaaca cgccaatcga                                                   20


<210>  43
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer minD_check_2_1

<400>  43
ttatcctccg aacaagcgtt tga                                               23


<210>  44
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer minD_check_2_2

<400>  44
atggcacgca ttattgttgt tac                                               23


