                         SEQUENCE LISTING

<110>  BioAmber Inc.
       RYAN, Owen
 
<120>  TOOLS AND METHODS FOR GENOME EDITING ISSATCHENKIA ORIENTALIS AND 
       OTHER INDUSTRIALLY USEFUL YEAST

<130>  12008-346

<150>  US 62/505,451
<151>  2017-05-12

<160>  72    

<170>  PatentIn version 3.5

<210>  1
<211>  279
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<222>  (1)..(279)
<223>  ARS-1

<400>  1
ctttggttct gataggaata aaaaagcaga taaacaattt gaaaaagcaa aaaagaactg       60

agcccgatgc ggggctcgaa cccgcagcct tttgattgca cttccttaac agaagaaatc      120

ttaagagtca aacgctctac cgattgagct aaccaggctt ttcttgaaat taaactttta      180

tggatttata tgttgtttgt agatatctga tgcctgctca ctatttaatc attatatgaa      240

acaaaagaac atctttcttc ttaaccattg tataaatat                             279


<210>  2
<211>  704
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<222>  (1)..(704)
<223>  ARS-2

<400>  2
atttatgaaa gcggaagttg agaagtggca gaacaatttt gaaatggtag ctgattctaa       60

tgatgttcta ctgagaagta ttaacttggt tgatcgtcca ttgctagatg attatttcag      120

agtcaccact acaactaaat cgctgttttt aagaacgttg cctatttcaa tatttgatat      180

tgcagtagag aaagaaaaca ttgtaattat gtaatgtgaa tctttaagag ttgtcactga      240

gtcactgaat aacagctaaa cttaattctt caatacaaaa aatagaacag cttcaagaaa      300

aataatggac gagaccggag tcgaaccgat gacctttcgc ttgcaaggcg aacgcgctac      360

caactacgcc acacgcccag taaaataaga atggtcccta gcaggatcga actgctgatc      420

ttggcgttat tagcaccacg ccttaaccaa ctgggccaag ggaccttttc ttgaagagga      480

agttttacgg ttttaaacca ctaacacaaa aaatatgtct tagtttgtat ttattctctt      540

attttttttt gtattagtaa attaatgaga aatgaaaatg aacaatgtga ataattgtct      600

ggaaaataat cttttcacgt agtgataaaa aattcaaagt ttcaagagaa acgaatatat      660

caaaatgtcg aaataaaaat tgttcaactt gacagattaa aact                       704


<210>  3

<400>  3
000

<210>  4
<211>  90
<212>  DNA
<213>  Issatchenkia orientalis

<400>  4
cgaacccgca gccttttgat tgcacttcct taacagaaga aatcttaaga gtcaaacgct       60

ctaccgattg agctaaccag gcttttcttg                                        90


<210>  5
<211>  45
<212>  DNA
<213>  Issatchenkia orientalis

<400>  5
taagagtcaa acgctctacc gattgagcta accaggcttt tcttg                       45


<210>  6
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus sequence for ARS-1


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  n is a, c, g, or t

<400>  6
taagagncnn angctctacc gattgagcta nccnggc                                37


<210>  7
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus sequence for ARS-1


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is C or T

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is A or T

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is A or C

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is C or T

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  n is G or A

<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  n is G or A

<400>  7
taagagncnn angctctacc gattgagcta nccnggc                                37


<210>  8
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Highly conserved sequence

<400>  8
gctctaccga ttgagcta                                                     18


<210>  9
<211>  43
<212>  DNA
<213>  Candida ethanolica

<400>  9
ttaagagtca aacgctctac cgattgagct agccaggctt ttc                         43


<210>  10
<211>  41
<212>  DNA
<213>  Candida intermedia

<400>  10
ttaagagtca aatgctctac cgattgagct aaccaggctt t                           41


<210>  11
<211>  46
<212>  DNA
<213>  Candida sorboxylosa

<400>  11
ttaagagtca aacgctctac cgattgagct aaccaggctt ttgttg                      46


<210>  12
<211>  47
<212>  DNA
<213>  Candida tanzawaensis

<400>  12
cttaagagtc aaacgctcta ccgattgagc taaccaggcc tttcttg                     47


<210>  13
<211>  40
<212>  DNA
<213>  Debaryomyces hansenii

<400>  13
ttaagagtca aacgctctac cgattgagct agccaggctt                             40


<210>  14
<211>  40
<212>  DNA
<213>  Leptosphaeria biglobosa

<400>  14
taagagtctc acgctctacc gattgagcta accaggcttt                             40


<210>  15
<211>  38
<212>  DNA
<213>  Leptosphaeria maculans

<400>  15
taagagtctc acgctctacc gattgagcta accaggct                               38


<210>  16
<211>  41
<212>  DNA
<213>  Metschnikowia australis

<400>  16
ttaagagtca aatgctctac cgattgagct aaccaggctt t                           41


<210>  17
<211>  47
<212>  DNA
<213>  Millerozyma farinosa

<400>  17
ttaagagtca aacgctctac cgattgagct agccaggctt cttgttg                     47


<210>  18
<211>  46
<212>  DNA
<213>  Nakazawaea peltata

<400>  18
taagagtcaa acgctctacc gattgagcta gccaggctat ttcttg                      46


<210>  19
<211>  47
<212>  DNA
<213>  Pichia kudriavzevii

<400>  19
cttaagagtc aaacgctcta ccgattgagc taaccaggct tttcttg                     47


<210>  20
<211>  46
<212>  DNA
<213>  Pichia membranifaciens

<400>  20
ttaagagtca aacgctctac cgattgagct aaccaggctt ttgttg                      46


<210>  21
<211>  47
<212>  DNA
<213>  Pichia sorbitophila

<400>  21
ttaagagtca aacgctctac cgattgagct agccaggctt gttcttg                     47


<210>  22
<211>  48
<212>  DNA
<213>  Scheffersomyces lignosus

<400>  22
cttaagagtc aaacgctcta ccgattgagc taaccaggca tgtttctt                    48


<210>  23
<211>  46
<212>  DNA
<213>  Scheffersomyces shehatae

<400>  23
ttaagagtca aacgctctac cgattgagct aaccaggcat ttgttg                      46


<210>  24
<211>  45
<212>  DNA
<213>  Scheffersomyces stipitis

<400>  24
taagagtcaa acgctctacc gattgagcta accaggcatt tcttg                       45


<210>  25
<211>  40
<212>  DNA
<213>  Spathaspora girioi

<400>  25
cttaagagtc aaacgctcta ccgattgagc taaccaggct                             40


<210>  26
<211>  45
<212>  DNA
<213>  Spathaspora gorwiae

<400>  26
cttaagagtc aaacgctcta ccgattgagc taaccaggca ttttc                       45


<210>  27
<211>  41
<212>  DNA
<213>  Spathaspora hagerdaliae

<400>  27
taagagtcaa acgctctacc gattgagcta accaggcttt t                           41


<210>  28
<211>  42
<212>  DNA
<213>  Spathaspora passalidarum

<400>  28
ttaagagtca aacgctctac cgattgagct aaccaggctt tt                          42


<210>  29
<211>  44
<212>  DNA
<213>  Sugiyamaella xylanicola

<400>  29
taagagtcaa acgctctacc gattgagcta accaggcttt tctt                        44


<210>  30
<211>  46
<212>  DNA
<213>  Wickerhamia fluorescens

<400>  30
ttaagagtca aacgctctac cgattgagct aaccaggctt ttgttg                      46


<210>  31
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus sequence from alignment of SEQ ID NOs: 9-30


<220>
<221>  misc_feature
<222>  (8)..(9)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  n is a, c, g, or t

<400>  31
taagagtnna ngctctaccg attgagctan ccaggc                                 36


<210>  32
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus sequence from alignment of SEQ ID NOs: 9-30


<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is A or T

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is A or C

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is C or T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  n is A or G

<400>  32
taagagtnna ngctctaccg attgagctan ccaggc                                 36


<210>  33
<211>  858
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<222>  (1)..(858)
<223>  Comprises TEF1 promoter

<400>  33
ctgcaactac agagccattc cttcacatgt atagaatata cacataaaga agagatgacg       60

attttacatc aagaaaaaaa cagccatttc tgcaaacttt taggtttagc cacagttttt      120

caacagaaaa aaagaagagt tatgttttta ttttttttac cttcgaaagc tatactataa      180

aagctacttt ccacattttt cagatgaata gcaaccccag ttacgtagat gtgttttggg      240

tcacctgcat agaaggtatt tgaaacatca tgaaaactgt ttcaccctct gtgaagcata      300

aacactagaa agccaatgaa gagctctaca agcctcatat gggttcaatg ggtctgcaat      360

gaccgcatac gggcttggac aattaccttc tattgaattt ctgagaagag atacatctga      420

ccagcaatgt aagcagacaa tcccaattct gtaaacaacc tctttgtcca taattcccca      480

tcagaagagt gaaaaatgcc ctcaaaatgc atgcgccaca cccacctctc aactgcactg      540

cgccacatct gagggtcctt tcaggggtcg actaccccgg acacctcgca gaggagcgac      600

gtcacgtact tttaaaatgg cagagacgcg cagtttcttg aagaaaggat aaaaatgaaa      660

tggtgcggaa atgcgaaaat gatgaaaaat tttcttggtg gcgaggaaat tgagtgcaat      720

aattggcacg aggttgttgc cacccgagtg tgagtatata tcctagtttc tgcacttttc      780

ttcttctttt ctttacgttt tcttttcaac ttttttttac tttttccttc aacagacaaa      840

tctaacttat atatcaca                                                    858


<210>  34
<211>  861
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<222>  (1)..(861)
<223>  Comprises TDH3 Promoter

<400>  34
gttggcgtat ctacatcact tcctacaaac aacaccacga attgcgtccg tggtgacgca       60

actacgaatg gcattgtcaa tgccaatgcc agtgcacata cacgtgcaag tcccaccggt      120

tccctgcccg gctatggtag agacaagaag gacgataccg gcatcgacat caacagtttc      180

aacagcaatg cgtttggcgt cgacgcgtcg atggggctgc cgtatttgga tttggacggg      240

ctagatttcg atatggatat ggatatggat atggatatgg agatgaattt gaatttagat      300

ttgggtcttg atttggggtt ggaattaaaa ggggataaca atgagggttt tcctgttgat      360

ttaaacaatg gacgtgggag gtgattgatt taacctgatc caaaaggggt gtgtctattt      420

tttagagtgt gtctttgtgt caaattatgg tagaatgtgt aaagtagtat aaactttcct      480

ctcaaatgac gaggtttaaa acaccccccg ggtgagccga gccgagaatg gggcaattgt      540

tcaatgtgaa atagaagtat cgagtgagaa acttgggtgt tggccagcca agggggaagg      600

aaaatggcgc gaatgctcag gtgagattgt tttggaattg ggtgaagcga ggaaatgagc      660

gacccggagg ttgtgacttt agtggcggag gaggacggag gaaaagccaa gagggaagtg      720

tatataaggg gagcaatttg ccaccaggat agaattggat gagttataat tctactgtat      780

ttattgtata atttatttct ccttttatat caaacacatt acaaaacaca caaaacacac      840

aaacaaacac aattacaaaa a                                                861


<210>  35
<211>  845
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<222>  (1)..(845)
<223>  Comprises PGK1 Promoter

<400>  35
ccaagaatgc cggtgatatg atcaacaaat actccatctt atacaacaga accagacaag       60

ccgtcattac taacgaattg gttgatatta ttactggtgc ttcctccttg aactagatgt      120

gctgaatgcc atggcaactc agagtcctac attgggactc tgaaattctc taggagcatg      180

cacacgtaat atacgtgtct gtctccacgc cgatatttgc tgcaacggca acatcaatgt      240

ccacgtttac acacctacat ttatatctat atttatattt atatttattt atttatgcta      300

cttagcttct atagttagtt aatgcactca cgatattcaa aattgacacc cttcaactac      360

tccctactat tgtctactac tgtctactac tcctctttac tatagctgct cccaataggc      420

tccaccaata ggctctgcca atacattttg cgccgccacc tttcaggttg tgtcactcct      480

gaaggaccat attgggtaat cgtgcaattt ctggaagaga gtccgcgaga agtgaggccc      540

ccactgtaaa tcctcgaggg ggcatggagt atggggcatg gaggatggag gagtgggggg      600

ggggggggga aaaataggta gcgaaaggac ccgctatcac cccacccgga gaactcgttg      660

ccgggaagtc atatttcgac actccgggga gtctataaaa ggcgggtttt gtcttttgcc      720

agttgatgtt gctgagagga cttgtttgcc gtttcttccg atttaacagt atagaatcaa      780

ccactgttaa ttatacacgt tatactaaca caacaaaaac aaaaacaacg acaacaacaa      840

caaca                                                                  845


<210>  36
<211>  829
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<222>  (1)..(829)
<223>  Comprises PGI1 Promoter

<400>  36
cctacacaga cattactagc cgtcattaga cgtagactta tacacctctc gatgggttct       60

catggcgcct tttgtcaacc tgtgtctctt tccaggacag cgtcggtagc atccacaatg      120

tccatctcag acaccgtcga tcaccatcaa ctgccgccac tacaatttcc atcacagaca      180

cagctaatac aaccaatcca accgccaccg ccaccacgcc cacaactctc tctggcgaat      240

ccaagggccc ttcgctcgcc atctatatct tcaacgaaca acggaattac aaacatgggc      300

agtagttcaa acaatctcca gactctcaac tctctctcgc tatcgttgaa acatccacag      360

ttccaaggcc tattctcccc actggatgtc cacagtccgt acgaacagaa cgttccttcc      420

ccactggccc ccaccgttcc ggctgttccg ggaaccgcac cttcattcga gtcggacgat      480

ctctacaatg caacggctgc ccgcaaaaga gactctctca agatgaagag aagatagacg      540

ctacatcatt gtctgtgcag tacctaatat atagtacttg gtataaggta taataaaact      600

ataaaattat aataatctta ataataataa ccatattaat ggaaggatga ggcccgatgt      660

cctttttttt gcctttctac tatagtgctt acattgtgta taaattctca tgattttcca      720

gctcgcatgt ttttcttata aaaatagccc agaccccata tctgcccata actttatatt      780

ccgttcccgg accaacttga gtaatacact cactacaaat agcgccgtc                  829


<210>  37
<211>  863
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<222>  (1)..(863)
<223>  Comprises PFK1 Promoter

<400>  37
ggcagtccta aagcaaggca ttgatcgatc aaaattcacc ttcaaggcat ggggccaacc       60

ttatttgtgt gcaatcactc taacggttgt tttcatcatg atatggatcg atggttatta      120

tgtcttcttg ccaggtagct ggtcgacatc gaccttcttg tttacatatt tgatgtgttt      180

tgttgacttt ggtattgtcg ttgcgtggaa attcattaaa ggcacaaaat atagatccaa      240

cccaatggaa gtggacttac atactggttt ggaggaagtt attttacatg agttgatgct      300

tgagaagaag agggcattgt atcaacagaa aagtccaatg gctagaacat gggaaagatt      360

gaatgaactc ttattcggca agaattaagc tcctccctta aagttctact ttttccttct      420

attttatacc accctactcc ttcaattact attatttttc gctgttttgc tttatttgat      480

ctacaactgt ataactgaca agacctgtaa taaatgcggc tttaatcatt taatttcttt      540

tccgaggatc acgtgcaagt cccagagatg gactactaac cgtgtgtgag actggtacaa      600

aaaagacgaa atggaataca ttatagagat gatgaggatt tgcccaaagt ggtgggaaaa      660

aaaaaacatg ggctgacgaa ggaaagctgc actccgaaga gcaccgcagt gcgtgagttt      720

gttgcggacg caatatatat atatataatc tacctctggt gcatattttc ttctttctta      780

tagtgcccgg acagtttcag tttgttgctt cggttataaa agaagaaaca aaaaaaagcg      840

gttagcaaca acagttcgca atc                                              863


<210>  38
<211>  846
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<222>  (1)..(846)
<223>  Comprises PDC1 Promoter

<400>  38
cagctctgtg ttgaagagac gtacaagatc cgcctttctg tcccttggcg acgccctgat       60

cccatggctt gccaacagcg ggcgaagatc cttcacccgc agcgtccatg gattgaactc      120

gggcgatagg tactgtgacg agtccatcgg ttcctgtcag atgggatact cttgacgtgg      180

aaaattcaaa cagaaaaaaa cccccaataa tgaaaaataa cactacgtta tatccgtggt      240

atcctctatc gtatcgtatc gtatcgtagc gtatcgtacc gtaccgtatt acagtatagt      300

ctaatattcc gtatcttatt gtatcctatc ctattcgatc ctattgtatt tcagtgcacc      360

attttaattt ctattgctat aatgtcctta ttagttgcca ctgtgaggtg accaatggac      420

gagggcgagc cgttcagaag ccgcgaaggg tgttcttccc atgaatttct taaggagggc      480

ggctcagctc cgagagtgat gcgagacgtc tcggttagcg tatccccctt cctcggcttt      540

tacaaatgat gcgctcttaa tagtgtgtcg ttatcctttt ggcattgacg ggggagggaa      600

attgattgag cgcatccata ttttggcgga ctgctgagga caatggtggt ttttccgggt      660

ggcgtgggct acaaatgata cgatggtttt ttcttttcgg agaaggcgta taaaaaggac      720

acggagaacc catttattct aataacagtt gagcttcttt aattatttgt taatataata      780

ttctattatt atatattttc ttcccaataa aacaaaataa aacaaaacac agcaaaacac      840

aaaaat                                                                 846


<210>  39
<211>  850
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<222>  (1)..(850)
<223>  Comprises HHF1 Promoter

<400>  39
ggttctagcc atggtgtggt tatattttat ttggagtatg taattaataa aggaacacac       60

aagggtaaaa taagcaaaaa ataaaataaa ataaaataaa cttggaaaaa ataaagggca      120

aaaaaaagtc caataaaaaa gagcgggggg ttcacggtat ttaaataatt ttccaagcgg      180

aaggtggaac aatagcagat ggccaataaa aaaaaagaaa caccacagct gatggaaatg      240

gcctgttagt acaaaggggg ggaaacggca gtctcgtagc agccaaaaaa aagggcgcga      300

attctcgtgc ttacgttccc cattgaatta agagtttctt aactatgtcg aatgcgcgtg      360

ctgaaaacta cggctaaaca agtgtagttg acatcacgca aggtttgccc ctctcctaat      420

ttcggtgagg ttttacgcga taaaaaaaaa ttgtagagat ggagaaaaaa tttctttaaa      480

ctgtcaatgg aaacaggagg aaaagaaact gttgaaagac aaaaatcgag gtgtgcggtt      540

tgagagctgc gcctatgcgg gcgcaagttc gggaacggtg taaggaaggg aagggggcgt      600

ttcagatagg tacatttgta tgtcattgtt ggtattgtat acatttctcg ccatcgtacc      660

ccattgtaaa tggatgagtc tctccatgta aatgtaaaat ttgatgagtg agaatatacg      720

acaattcctt taaaaggggc caatgtccgc actggattta gggttgatat aagtcacttt      780

taataggaga atttttcctt attgtccttg tattgtacac aacaaaagaa acccatacaa      840

cagatataca                                                             850


<210>  40
<211>  881
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<222>  (1)..(881)
<223>  Comprises ENO1 Promoter

<400>  40
ggagaaatgc agacagtcaa tgaacacaac tgtctcaata tgcatctatg cacatgcaca       60

cacgcacaca tcacaggtac ccctacaaag agaggtccct tgataatgtt tcattaccac      120

gtggcatccc cccccccaat aaacaagtgg ccgagttccc ctgttgcaga ggaggacaaa      180

agaaccgctg gtgttggtac cattatgcag caactagcac aacaaacaac cgacccagac      240

atacaaatca acaacacttc gccaaagaca ccctttccag ggaggatcca ctcccaacgt      300

ctctccataa tgtctctgtt ggcccatgtc tctgtcgttg acaccgtaac cacaccaacc      360

aacccgtcca ttgtactggg atggtcgtcc atagacacct ctccaacggg gaacacctca      420

ttcgtaaacc gccaaggtta ccgttcctcc tgactcgccc cgttgttgat gctgcgcacc      480

tgtggttgcc caacatggtt gtatatcgtg taaccacacc aacacatgtg cagcacatgt      540

gtttaaaaga gtgtcatgga ggtggatcat gatggaagtg gactttacca cttgggaact      600

gtctccactc ccgggaagaa aagacccggc gtatcacgcg gttgcctcaa tggggcaatt      660

tggaaggaga aatataggga aaatcacgtc gctctcggac ggggaagagt tccagactat      720

gagggggggg gggggtggta tataaagaca ggagatgtcc accccaagag agaggaagaa      780

gttggaactt tagaagagag agataacttt ccccagtgtc catcaataca caaccaaaca      840

caaactctat atatacacat ataaccccct ccaaccaaac a                          881


<210>  41
<211>  832
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<222>  (1)..(832)
<223>  Comprises CCW12 Promoter

<400>  41
ctcttctgac gggtttcctg tcgcttgcga gtgcgtcagc tgcatttgtc aagagatacg       60

ataactcaac atcttcgtgt gtgccaacaa cgatctcttc tacttcgatt gtttctgttt      120

acggtcctca gtttactggt gtgccatcaa ataagtacgt tgttccaacc gttgttggtg      180

acttggtttc aaacgatgct ccagaattca ccgtttacat tcctcagtcc ttgtacaatt      240

taaccggttt ggaattagag ttaacttcag cggttggttt cgatgtgtca ccagaatctt      300

atactttata ttcaggtgac attggcgatt ttgtttaccc aggccaaatt tatgaaacat      360

caaatactgg tttggttttt gacggtagaa cgcaagatcc tttactaaag attaccatta      420

ttggtatccc tgaagcaaac caaccagttt tcattgctga ttttgttctc actttagatg      480

taatttcatc atctggctta caaaagaggg acaccttaac ttttaatttg tcattctcta      540

ttaagaattc ggcatatatc tcctcgtctt ctgttgcatc aacaagtgaa tcctcccaag      600

ctactactgg tgctactact ggtgctacta ctggtgctac tactggtgaa actactggtg      660

ctactactgg tgctactact ggtgcttact ggtgctacta ctggtgctac tactggtgct      720

actactggtg aaactaccgg agctactacc ggagctactg gtgaaactac cggagctact      780

accggagcta ctaccggtgc tactactggt gcaattactt ctgagctcac ta              832


<210>  42
<211>  894
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<222>  (1)..(894)
<223>  Comprises ACT1 Promoter

<400>  42
gtggattatc gttattgagg tggtcattta tcattgacga ctttctcaag aagcggcact       60

ttttcacctt caccattccc cccctttccg ttccttgtta actaacaact tctttccttg      120

tctcctctct atttgatacc ttggtctccc tgtgtttgtt tttgtttgtg gtggttgtgt      180

gcacacgtgt atgtttgttt gtttgtgtgt gtgttttgtt ttatcttttc gtgattttga      240

gacaattgac tcttcagtta aagacgggaa tttatggatt cattgatagt ttatttgatg      300

atatatggtc tgtcatatat atttgaagga aaatttatga agaacctctt ttagacacaa      360

accatcaact agtttaatct ggatacaact tatcaagata ttccttttat ttggtttgat      420

tgatttgatg aagatccaga tatacattgt ctttgttttt accaaagtaa tcaagggttc      480

tcaattcctt agtggttata tttatagctt gtgattatgt ttttcccccc ccgtgtttgc      540

caatctcaca actatacaaa aagaaataga gaaatatgac aattatgcca taatacaaaa      600

agtggtatta tccttatttt ttattccatc caccaattcc ttctattcag tagaaacaaa      660

aagcattttc taacttatag ttcattttga ccaattcacg ttcttggtga ttttgtttat      720

ttgtccatta cgaagagaat cacaaaacag aacaaacagt acaaacaaac aaacaaacaa      780

acaaacaaac aaaggagaaa acattttgga aaataaaatt aaagcaacgt actaacattt      840

aaccattccc cctattattg ttttctatag ttagttattg ataatggttc tggt            894


<210>  43
<211>  217
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<222>  (1)..(217)
<223>  Comprises ADH1 Terminator

<400>  43
tgggctgact tgggtgtact ggtgtgacgt ttttatgtgt atattgatat gcatggggga       60

tgtatagtga tgaggagtag agtatataac gaaatgaaat gaaataatat gataagataa      120

gataagataa gatcaaataa gataatataa gatgcgacat gaggagttca atgtagcata      180

ctacacgatg ctgcagtaca actctgatac gctagac                               217


<210>  44
<211>  209
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<222>  (1)..(209)
<223>  TDH3 Terminator

<400>  44
gcggcgaatc tctggctcat gggggatatc ctctttggct tttttttccc attctctttg       60

ttttgattat ctaatgactc attgggagga ttttctcact tcaagctttt ttttcttgca      120

ctctttcata actccagctc tctctaactg aggctacaat gccttttaac gaacttatga      180

gacgtttcta aattatatag gtatatgcc                                        209


<210>  45
<211>  73
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Threonine

<400>  45
gctcgtatgg ccaagttggt aaggcgctac actagtaatg tagcgatcct cagttcgact       60

ctgagtgcga gca                                                          73


<210>  46
<211>  84
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Leucine

<400>  46
ggagggatgg ccgagtggtc taaggcggca gacttaagat ctgttggacg catgtccgcg       60

cgagttcgaa cctcgcttcc ttca                                              84


<210>  47
<211>  72
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Proline

<400>  47
gggttaatgg tctagtggta tgattctcgc tttgggtgcg agaggccctg ggttcaattc       60

ccagttgacc cc                                                           72


<210>  48
<211>  74
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Methionine

<400>  48
gctttggtgg cccagttggt taaggcgtca gtctcataat ctgaagatcg cgagttcgaa       60

tctcgcctag agca                                                         74


<210>  49
<211>  72
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Glutamine

<400>  49
tccgatatag tgtaacggct atcacggtcc gctttcaccg ggcagacccg ggttcgactc       60

ccggtatcgg aa                                                           72


<210>  50
<211>  72
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Glutamate

<400>  50
aggtcgtacc cggattcgaa ccggggttgg tcggatcaaa accgacagtg ataaccacta       60

cactatacaa cc                                                           72


<210>  51
<211>  74
<212>  DNA
<213>  tRNA Valine


<220>
<221>  misc_feature
<223>  tRNA Valine

<400>  51
ggtcggatgg tctagttggt tatggcatat gcttaacacg cataacgtcc ccagttcgat       60

cctgggttcg atca                                                         74


<210>  52
<211>  82
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Serine

<400>  52
ggcaatttgt ccgagtggtt aaggagaaag attagaaatc ttttgggctt tgcccgcgca       60

ggttcgaatc ctgcagttgt cg                                                82


<210>  53
<211>  72
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Histidine

<400>  53
gccgttctag tatagtggtc agtacgcatc gttgtggccg atgagaccca ggttcgattc       60

ctgggaacgg ca                                                           72


<210>  54
<211>  72
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Phenylalanine

<400>  54
gcgggcttag ctcagtggga gagcgccaga ctgaagatct ggaggccctg tgttcgatcc       60

acagagctcg ca                                                           72


<210>  55
<211>  73
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Arginine

<400>  55
gcccgtgtag cgtaatggtt aacgcgtttg acttctaatc aaaagattct gggttcgact       60

cccagcatgg gtg                                                          73


<210>  56
<211>  73
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Alanine

<400>  56
gggcgtgtgg cgtagttggt agcgcgttcg ccttgcaagc gaaaggtcat cggttcgact       60

ccggtctcgt cca                                                          73


<210>  57
<211>  74
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Isoleucine

<400>  57
ggtcccttgg cccagttggt taaggcgtgg tgctaataac gccaagatca gcagttcgat       60

cctgctaggg acca                                                         74


<210>  58
<211>  72
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Asparagine

<400>  58
ctccgagacc gggaattgaa cccgggtctc ccgcgtgaca agcggaaatt ctagccacta       60

aactatctcg ga                                                           72


<210>  59
<211>  72
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Cysteine

<400>  59
agcccgcggc cgggtttgaa ccggcgacca acagatttgc aatctgctgc tctaccactg       60

agctacgcgt gc                                                           72


<210>  60
<211>  72
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Tryptophan

<400>  60
ggggctatgg ctcaatggta gagctttcga ctccagatcg aagggttgca ggttcgattc       60

ctgttggcct ca                                                           72


<210>  61
<211>  176
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Threonine (SEQ ID NO: 45) + 100-bp 5' genomic DNA sequence

<400>  61
gatatcctta tgctttgcaa atcacatatg cagtagtaga atagacaaac tggaccacaa       60

ttgcatttta tagaatagtg gaattttgga cacacaacaa accgctcgta tggccaagtt      120

ggtaaggcgc tacactagta atgtagcgat cctcagttcg actctgagtg cgagca          176


<210>  62
<211>  180
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Leucine (SEQ ID NO: 46) + 100-bp 5' genomic DNA sequence

<400>  62
ctcagggaat taacgtataa aaatatataa gatagaaata agcaagaatc agattcaaga       60

gtaggtacta ccagccccct atgaatttta tcaattggag ggatggccga gtggtctaag      120

gcggcagact taagatctgt tggacgcatg tccgcgcgag ttcgaacctc gcttccttca      180


<210>  63
<211>  199
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  misc_feature
<223>  tRNA Proline (SEQ ID NO: 47) + 100-bp 5' genomic DNA sequence

<400>  63
atgttctcag agaattaacg tataaaaata tataagatat aaataagcaa taatcagatt       60

ctaaagtacg caccaccagc aacactttca cattcataag cccagcaaca ctttcacatt      120

cataagcggg ttaatggtct agtggtatga ttctcgcttt gggtgcgaga ggccctgggt      180

tcaattccca gttgacccc                                                   199


<210>  64
<211>  89
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  misc_feature
<223>  S. cerevisiae tRNA Tyrosine

<400>  64
ctctcggtag ccaagttggt ttaaggcgca agactgtaat ttatcactac gaaatcttga       60

gatcgggcgt tcgactcgcc cccgggaga                                         89


<210>  65
<211>  89
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  misc_feature
<223>  S. cerevisiae tRNA Phenylalanine

<400>  65
ggatttagct cagttgggag agcgccagac tgaagaaaaa cttcggtcaa gtcatctgga       60

ggtcctgtgt tcgatccaca gaattcgca                                         89


<210>  66
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I. orientalis tRNA consensus sequence TGGnCnAGT


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is a, c, g, or t

<400>  66
tggncnagt                                                                9


<210>  67
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I. orientalis tRNA consensus sequence GTTCnAnnC


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  n is a, c, g, or t

<400>  67
gttcnannc                                                                9


<210>  68
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I. orientalis tRNA consensus sequence GnTCnAnnC


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  n is a, c, g, or t

<400>  68
gntcnannc                                                                9


<210>  69
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I. orientalis tRNA consensus sequence GTTCnAnnC


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  n is a, c, g, or t

<400>  69
gttcnannc                                                                9


<210>  70
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus sequence for ARS-2


<220>
<221>  misc_feature
<222>  (9)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(37)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (40)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (42)..(42)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<400>  70
tggacgagnn nngantcgaa cnnanganct tncnnnngcn angnnaacgc gctaccaact       60

acgccacacg ccc                                                          73


<210>  71
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus sequence for ARS-2


<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (45)..(45)
<223>  n is a, c, g, or t

<400>  71
tggacgagac cggagtcgaa ccgatganct ttcgcttgca aggcnaacgc gctaccaact       60

acgccacacg ccc                                                          73


<210>  72
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus sequence for ARS-2

<400>  72
aacgcgctac caactacgcc acacgccc                                          28


