                         SEQUENCE LISTING

<110>  MELMAN, Arnold
       CHRIST, George
       ANDERSSON, Karl-Erik
 
<120>  COMPOSITIONS AND METHODS FOR TREATING IDIOPATHIC OVERACTIVE 
       BLADDER SYNDROME AND DETRUSOR OVERACTIVITY

<130>  IONC-002/001WO

<150>  62/505,382
<151>  2017-05-12

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  508
<212>  DNA
<213>  human cytomegalovirus

<400>  1
cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt       60

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      120

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      180

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      240

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      300

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      360

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      420

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      480

acggtgggag gtctatataa gcagagct                                         508


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 priming site

<400>  2
taatacgact cactataggg                                                   20


<210>  3
<211>  239
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BGH polyA

<400>  3
agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc       60

cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc      120

gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg      180

ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta gtgggctct       239


<210>  4
<211>  679
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC origin of replication

<400>  4
ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct       60

tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa      120

ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag      180

tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc      240

tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg      300

actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca      360

cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat      420

gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg      480

tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc      540

ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc      600

ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctgggc ttttgctggc      660

cttttgctca catgttctt                                                   679


<210>  5
<211>  800
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  kanamycin resistance marker

<400>  5
ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc       60

tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc      120

tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg      180

aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag      240

ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg      300

ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg      360

caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac      420

atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg      480

acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc      540

ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg      600

aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc      660

aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc      720

gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc      780

ttcttgacga gttcttctga                                                  800


<210>  6
<211>  3779
<212>  DNA
<213>  homo sapiens

<400>  6
atggcaaacg gtggcggcgg cggcggcggc agcagcggcg gcggcggcgg cggcggcgga       60

ggcagcggtc ttagaatgag cagcaatatc cacgcgaacc atctcagcct agacgcgtcc      120

tcctcctcct cctcctcctc ttcctcttct tcttcttcct cctcctcttc ctcctcgtcc      180

tcggtccacg agcccaagat ggatgcgctc atcatcccgg tgaccatgga ggtgccgtgc      240

gacagccggg gccaacgcat gtggtgggct ttcctggcct cctccatggt gactttcttc      300

gggggcctct tcatcatctt gctctggcgg acgctcaagt acctgtggac cgtgtgctgc      360

cactgcgggg gcaagacgaa ggaggcccag aagattaaca atggctcaag ccaggcggat      420

ggcactctca aaccagtgga tgaaaaagag gaggcagtgg ccgccgaggt cggctggatg      480

acctccgtga aggactgggc gggggtgatg atatccgccc agacactgac tggcagagtc      540

ctggttgtct tagtctttgc tctcagcatc ggtgcacttg taatatactt catagattca      600

tcaaacccaa tagaatcctg ccagaatttc tacaaagatt tcacattaca gatcgacatg      660

gctttcaacg tgttcttcct tctctacttt ggcttgcggt ttattgcagc caacgataaa      720

ttgtggttct ggctggaagt gaactctgta gtggatttct tcacggtgcc ccccgtgttt      780

gtgtctgtgt acttaaacag aagttggctt ggtttgagat ttttaagagc tctgagactg      840

atacagtttt cagaaatttt gcagtttctg aatattctta aaacaagtaa ttccatcaag      900

ctggtgaatc tgctctccat atttatcagc acgtggctga ctgcagctgg gttcatccat      960

ttggtggaga attcagggga cccatgggaa aatttccaaa acaaccaggc tctcacctac     1020

tgggaatgtg tcatttactc atggtcacaa tgtccaccgt tggttatggg gatgtttatg     1080

caaaaaccac acttcggcgc ctcttcatgg tcttcttcat cctcggggga ctggccatgt     1140

ttgccagcta cgtccctgaa atcatagagt taataggaaa ccgcaagaaa tacgggggct     1200

cctatagtgc ggttagtgga agaaagcaca ttgtggtctg cggacacatc actctggaga     1260

gtgtttccaa cttcctgaag gactttctgc acaaggaccg ggatgacgtc aatgtggaga     1320

tcgtttttct tcacaacatc tcccccaacc tggagcttga agctctgttc aaacgacatt     1380

ttactcaggt ggaattttat cagggttccg tcctcaatcc acatgatctt gcaagagtca     1440

agatagagtc agcagatgca tgcctgatcc ttgccaacaa gtactgcgct gacccggatg     1500

cggaggatgc ctcgaatatc atgagagtaa tctccataaa gaactaccat ccgaagataa     1560

gaatcatcac tcaaatgctg cagtatcaca acaaggccca tctgctaaac atccgagctg     1620

gaattggaaa gaaggtgatg acgcaatctg cctcgcagag ttgaagttgg gcttcatagc     1680

ccagagctgc ctggctcaag gcctctccac catgcttgcc aaccttctcc atgaggtcat     1740

tcataaagat tgaggaagac acatggcaga aatactactt ggaaggagtc tcaaatcaaa     1800

tgtacacaga atatctctcc agtgccttcg tgggtctgtc cttccctact gtttgtgagc     1860

tgtgttttgt gaagctcaag ctcctaatga tagccattga gtacaagtct gccaaccgag     1920

agagccgtat attaattaat cctggaaacc attttaagat ccaagaaggt actttaggat     1980

ttttcatcgc aagtgatgcc aaagaagtta aaagggcatt tttttactgc aaggcctgtc     2040

atgatgacat cacagatccc aaaagaataa aaaaatgtgg ctgcaaacgg cttgaagatg     2100

agcagccgtc aacactatca ccaaaaaaaa agcaacggaa tggaggcatg cggaactcac     2160

ccaacacctc gcctaagctg atgaggcatg accccttgtt aattcctggc aatgatcaga     2220

ttgacaacat ggactccaat gtgaagaagt acgactctac tgggatgttt cactggtgtg     2280

cacccaagga gatagagaaa gtcatcctga ctcgaagtga agctgccatg accgtcctga     2340

gtggccatgt cgtggtctgc atctttggcg acgtcagctc agccctgatc ggcctccgga     2400

acctggtgat gccgctccgt gccagcaact ttcattacca tgagctcaag cacattgtgt     2460

ttgtgggctc tattgagtac ctcaagcggg aatgggagac gcttcataac ttccccaaag     2520

tgtccatatt gcctggtacg ccattaagtc gggctgattt aagggctgtc aacatcaacc     2580

tctgtgacat gtgcgttatc ctgtcagcca atcagaataa tattgatgat acttcgctgc     2640

aggacaagga atgcatcttg gcgtcactca acatcaaatc tatgcagttt gatgacagca     2700

tcggagtctt gcaggctaat tcccaagggt tcacacctcc aggaatggat agatcctctc     2760

cagataacag cccagtgcac gggatgttac gtcaaccatc catcacaact ggggtcaaca     2820

tccccatcat cactgaacta gtgaacgata ctaatgttca gtttttggac caagacgatg     2880

atgatgaccc tgatacagaa ctgtacctca cgcagccctt tgcctgtggg acagcatttg     2940

ccgtcagtgt cctggactca ctcatgagcg cgacgtactt caatgacaat atcctcaccc     3000

tgatacggac cctggtgacc ggaggagcca cgccggagct ggaggctctg attgctgagg     3060

aaaacgccct tagaggtggc tacagcaccc cgcagacact ggccaatagg gaccgctgcc     3120

gcgtggccca gttagctctg ctcgatgggc catttgcgga cttaggggat ggtggttgtt     3180

atggtgatct gttctgcaaa gctctgaaaa catataatat gctttgtttt ggaatttacc     3240

ggctgagaga tgctcacctc agcaccccca gtcagtgcac aaagaggtat gtcatcacca     3300

acccgcccta tgagtttgag ctcgtgccga cggacctgat cttctgctta atgcagtttg     3360

accacaatgc cggccagtcc cgggccagcc tgtcccattc ctcccactcg tcgcagtcct     3420

ccagcaagaa gagctcctct gttcactcca tcccatccac agcaaaccga cagaaccggc     3480

ccaagtccag ggagtcccgg gacaaacaga agtacgtgca ggaagagcgg ctttgatatg     3540

tgtatccacc gccactgtgt gaaactgtat ctgccactca tttccccagt tggtgtttcc     3600

aacaaagtaa ctttccctgt tttcccctgt agtccccccc ttttttttta cacatatttg     3660

catatgtatg atagtgtgca tgtggttgtc atttttattt caccaccata aaacccttga     3720

gcacaacagc aaataagcag acgggctccg gaattcctgc agcccggggg atccactag      3779


<210>  7
<211>  3534
<212>  DNA
<213>  Homo sapiens

<400>  7
atggcaaatg gtggcggcgg cggcggcggc agcagcggcg gcggcggcgg cggcggaggc       60

agcagtctta gaatgagtag caatatccac gcgaaccatc tcagcctaga cgtgtcctcc      120

tcctcctcct cctcctcttc ctcttcttct tcttcctcct cctcttcctc ctcgtcctcg      180

gtccacgagc ccaagatgga tgcgctcatc atcccggtga ccatggaggt gccgtgcgac      240

agccggggcc aacgcatgtg gtgggctttc ctggcctcct ccatggtgac tttcttcggg      300

ggcctcttca tcatcttgct ctggcggacg ctcaagtacc tgtggaccgt gtgctgccac      360

tgcgggggca agacgaagga ggcccagaag attaacaatg gctcaagcca ggcggatggc      420

actctcaaac cagtggatga aaaagaggag gcagtggccg ccgaggtcgg ctggatgacc      480

tccgtgaagg actgggcggg ggtgatgata tccgcccaga cactgactgg cagagtcctg      540

gttgtcttag tctttgctct cagcatcggt gcacttgtaa tatacttcat agattcatca      600

aacccaatag aatcctgcca gaatttctac aaagatttca cattacagat cgacatggct      660

ttcaacgtgt tcttccttct ctacttcggc ttgcggttta ttgcagccaa cgataaattg      720

tggttctggc tggaagtgaa ctctgtagtg gatttcttca cggtgccccc cgtgtttgtg      780

tctgtgtact taaacagaag ttggcttggt ttgagatttt taagagctct gagactgata      840

cagttttcag aaattttgca gtttctgaat attcttaaaa caagtaattc catcaagctg      900

gtgaatctgc tctccatatt tatcagcacg tggctgactg cagccgggtt catccatttg      960

gtggagaatt caggggaccc atgggaaaat ttccaaaaca accaggctct cacctactgg     1020

gaatgtgtct atttactcat ggtcacaatg tccaccgttg gttatgggga tgtttatgca     1080

aaaaccacac ttgggcgcct cttcatggtc ttcttcatcc tcgggggact ggccatgttt     1140

gccagctacg tccctgaaat catagagtta ataggaaacc gcaagaaata cgggggctcc     1200

tatagtgcgg ttagtggaag aaagcacatt gtggtctgcg gacacatcac tctggagagt     1260

gtttccaact tcctgaagga ctttctgcac aaggaccggg atgacgtcaa tgtggagatc     1320

gtttttcttc acaacatctc ccccaacctg gagcttgaag ctctgttcaa acgacatttt     1380

actcaggtgg aattttatca gggttccgtc ctcaatccac atgatcttgc aagagtcaag     1440

atagagtcag cagatgcatg cctgatcctt gccaacaagt actgcgctga cccggatgcg     1500

gaggatgcct cgaatatcat gagagtaatc tccataaaga actaccatcc gaagataaga     1560

atcatcactc aaatgctgca gtatcacaac aaggcccatc tgctaaacat cccgagctgg     1620

aattggaaag aaggtgatga cgcaatctgc ctcgcagagt tgaagttggg cttcatagcc     1680

cagagctgcc tggctcaagg cctctccacc atgcttgcca acctcttctc catgaggtca     1740

ttcataaaga ttgaggaaga cacatggcag aaatactact tggaaggagt ctcaaatgaa     1800

atgtacacag aatatctctc cagtgccttc gtgggtctgt ccttccctac tgtttgtgag     1860

ctgtgttttg tgaagctcaa gctcctaatg atagccattg agtacaagtc tgccaaccga     1920

gagagccgta tattaattaa tcctggaaac catcttaaga tccaagaagg tactttagga     1980

tttttcatcg caagtgatgc caaagaagtt aaaagggcat ttttttactg caaggcctgt     2040

catgatgaca tcacagatcc caaaagaata aaaaaatgtg gctgcaaacg gcttgaagat     2100

gagcagccgt caacactatc accaaaaaaa aagcaacgga atggaggcat gcggaactca     2160

cccaacacct cgcctaagct gatgaggcat gaccccttgt taattcctgg caatgatcag     2220

attgacaaca tggactccaa tgtgaagaag tacgactcta ctgggatgtt tcactggtgt     2280

gcacccaagg agatagagaa agtcatcctg actcgaagtg aagctgccat gaccgtcctg     2340

agtggccatg tcgtggtctg catctttggc gacgtcagct cagccctgat cggcctccgg     2400

aacctggtga tgccgctccg tgccagcaac tttcattacc atgagctcaa gcacattgtg     2460

tttgtgggct ctattgagta cctcaagcgg gaatgggaga cgcttcataa cttccccaaa     2520

gtgtccatat tgcctggtac gccattaagt cgggctgatt taagggctgt caacatcaac     2580

ctctgtgaca tgtgcgttat cctgtcagcc aatcagaata atattgatga tacttcgctg     2640

caggacaagg aatgcatctt ggcgtcactc aacatcaaat ctatgcagtt tgatgacagc     2700

atcggagtct tgcaggctaa ttcccaaggg ttcacacctc caggaatgga tagatcctct     2760

ccagataaca gcccagtgca cgggatgtta cgtcaaccat ccatcacaac tggggtcaac     2820

atccccatca tcactgaact agtgaacgat actaatgttc agtttttgga ccaagacgat     2880

gatgatgacc ctgatacaga actgtacctc acgcagccct ttgcctgtgg gacagcattt     2940

gccgtcagtg tcctggactc actcatgagc gcgacgtact tcaatgacaa tatcctcacc     3000

ctgatacgga ccctggtgac cggaggagcc acgccggagc tggaggctct gattgctgag     3060

gaaaacgccc ttagaggtgg ctacagcacc ccgcagacac tggccaatag ggaccgctgc     3120

cgcgtggccc agttagctct gctcgatggg ccatttgcgg acttagggga tggtggttgt     3180

tatggtgatc tgttctgcaa agctctgaaa acatataata tgctttgttt tggaatttac     3240

cggctgagag atgctcacct cagcaccccc agtcagtgca caaagaggta tgtcatcacc     3300

aacccgccct atgagtttga gctcgtgccg acggacctga tcttctgctt aatgcagttt     3360

gaccacaatg ccggccagtc ccgggccagc ctgtcccatt cctcccactc gtcgcagtcc     3420

tccagcaaga agagctcctc tgttcactcc atcccatcca cagcaaaccg acagaaccgg     3480

cccaagtcca gggagtcccg ggacaaacag aagtacgtgc aggaagagcg gctt           3534


<210>  8
<211>  1178
<212>  PRT
<213>  homo sapiens

<400>  8

Met Ala Asn Gly Gly Gly Gly Gly Gly Gly Ser Ser Gly Gly Gly Gly 
1               5                   10                  15      


Gly Gly Gly Gly Ser Ser Leu Arg Met Ser Ser Asn Ile His Ala Asn 
            20                  25                  30          


His Leu Ser Leu Asp Val Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser 
        35                  40                  45              


Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Val His Glu Pro 
    50                  55                  60                  


Lys Met Asp Ala Leu Ile Ile Pro Val Thr Met Glu Val Pro Cys Asp 
65                  70                  75                  80  


Ser Arg Gly Gln Arg Met Trp Trp Ala Phe Leu Ala Ser Ser Met Val 
                85                  90                  95      


Thr Phe Phe Gly Gly Leu Phe Ile Ile Leu Leu Trp Arg Thr Leu Lys 
            100                 105                 110         


Tyr Leu Trp Thr Val Cys Cys His Cys Gly Gly Lys Thr Lys Glu Ala 
        115                 120                 125             


Gln Lys Ile Asn Asn Gly Ser Ser Gln Ala Asp Gly Thr Leu Lys Pro 
    130                 135                 140                 


Val Asp Glu Lys Glu Glu Ala Val Ala Ala Glu Val Gly Trp Met Thr 
145                 150                 155                 160 


Ser Val Lys Asp Trp Ala Gly Val Met Ile Ser Ala Gln Thr Leu Thr 
                165                 170                 175     


Gly Arg Val Leu Val Val Leu Val Phe Ala Leu Ser Ile Gly Ala Leu 
            180                 185                 190         


Val Ile Tyr Phe Ile Asp Ser Ser Asn Pro Ile Glu Ser Cys Gln Asn 
        195                 200                 205             


Phe Tyr Lys Asp Phe Thr Leu Gln Ile Asp Met Ala Phe Asn Val Phe 
    210                 215                 220                 


Phe Leu Leu Tyr Phe Gly Leu Arg Phe Ile Ala Ala Asn Asp Lys Leu 
225                 230                 235                 240 


Trp Phe Trp Leu Glu Val Asn Ser Val Val Asp Phe Phe Thr Val Pro 
                245                 250                 255     


Pro Val Phe Val Ser Val Tyr Leu Asn Arg Ser Trp Leu Gly Leu Arg 
            260                 265                 270         


Phe Leu Arg Ala Leu Arg Leu Ile Gln Phe Ser Glu Ile Leu Gln Phe 
        275                 280                 285             


Leu Asn Ile Leu Lys Thr Ser Asn Ser Ile Lys Leu Val Asn Leu Leu 
    290                 295                 300                 


Ser Ile Phe Ile Ser Thr Trp Leu Thr Ala Ala Gly Phe Ile His Leu 
305                 310                 315                 320 


Val Glu Asn Ser Gly Asp Pro Trp Glu Asn Phe Gln Asn Asn Gln Ala 
                325                 330                 335     


Leu Thr Tyr Trp Glu Cys Val Tyr Leu Leu Met Val Thr Met Ser Thr 
            340                 345                 350         


Val Gly Tyr Gly Asp Val Tyr Ala Lys Thr Thr Leu Gly Arg Leu Phe 
        355                 360                 365             


Met Val Phe Phe Ile Leu Gly Gly Leu Ala Met Phe Ala Ser Tyr Val 
    370                 375                 380                 


Pro Glu Ile Ile Glu Leu Ile Gly Asn Arg Lys Lys Tyr Gly Gly Ser 
385                 390                 395                 400 


Tyr Ser Ala Val Ser Gly Arg Lys His Ile Val Val Cys Gly His Ile 
                405                 410                 415     


Thr Leu Glu Ser Val Ser Asn Phe Leu Lys Asp Phe Leu His Lys Asp 
            420                 425                 430         


Arg Asp Asp Val Asn Val Glu Ile Val Phe Leu His Asn Ile Ser Pro 
        435                 440                 445             


Asn Leu Glu Leu Glu Ala Leu Phe Lys Arg His Phe Thr Gln Val Glu 
    450                 455                 460                 


Phe Tyr Gln Gly Ser Val Leu Asn Pro His Asp Leu Ala Arg Val Lys 
465                 470                 475                 480 


Ile Glu Ser Ala Asp Ala Cys Leu Ile Leu Ala Asn Lys Tyr Cys Ala 
                485                 490                 495     


Asp Pro Asp Ala Glu Asp Ala Ser Asn Ile Met Arg Val Ile Ser Ile 
            500                 505                 510         


Lys Asn Tyr His Pro Lys Ile Arg Ile Ile Thr Gln Met Leu Gln Tyr 
        515                 520                 525             


His Asn Lys Ala His Leu Leu Asn Ile Pro Ser Trp Asn Trp Lys Glu 
    530                 535                 540                 


Gly Asp Asp Ala Ile Cys Leu Ala Glu Leu Lys Leu Gly Phe Ile Ala 
545                 550                 555                 560 


Gln Ser Cys Leu Ala Gln Gly Leu Ser Thr Met Leu Ala Asn Leu Phe 
                565                 570                 575     


Ser Met Arg Ser Phe Ile Lys Ile Glu Glu Asp Thr Trp Gln Lys Tyr 
            580                 585                 590         


Tyr Leu Glu Gly Val Ser Asn Glu Met Tyr Thr Glu Tyr Leu Ser Ser 
        595                 600                 605             


Ala Phe Val Gly Leu Ser Phe Pro Thr Val Cys Glu Leu Cys Phe Val 
    610                 615                 620                 


Lys Leu Lys Leu Leu Met Ile Ala Ile Glu Tyr Lys Ser Ala Asn Arg 
625                 630                 635                 640 


Glu Ser Arg Ile Leu Ile Asn Pro Gly Asn His Leu Lys Ile Gln Glu 
                645                 650                 655     


Gly Thr Leu Gly Phe Phe Ile Ala Ser Asp Ala Lys Glu Val Lys Arg 
            660                 665                 670         


Ala Phe Phe Tyr Cys Lys Ala Cys His Asp Asp Ile Thr Asp Pro Lys 
        675                 680                 685             


Arg Ile Lys Lys Cys Gly Cys Lys Arg Leu Glu Asp Glu Gln Pro Ser 
    690                 695                 700                 


Thr Leu Ser Pro Lys Lys Lys Gln Arg Asn Gly Gly Met Arg Asn Ser 
705                 710                 715                 720 


Pro Asn Thr Ser Pro Lys Leu Met Arg His Asp Pro Leu Leu Ile Pro 
                725                 730                 735     


Gly Asn Asp Gln Ile Asp Asn Met Asp Ser Asn Val Lys Lys Tyr Asp 
            740                 745                 750         


Ser Thr Gly Met Phe His Trp Cys Ala Pro Lys Glu Ile Glu Lys Val 
        755                 760                 765             


Ile Leu Thr Arg Ser Glu Ala Ala Met Thr Val Leu Ser Gly His Val 
    770                 775                 780                 


Val Val Cys Ile Phe Gly Asp Val Ser Ser Ala Leu Ile Gly Leu Arg 
785                 790                 795                 800 


Asn Leu Val Met Pro Leu Arg Ala Ser Asn Phe His Tyr His Glu Leu 
                805                 810                 815     


Lys His Ile Val Phe Val Gly Ser Ile Glu Tyr Leu Lys Arg Glu Trp 
            820                 825                 830         


Glu Thr Leu His Asn Phe Pro Lys Val Ser Ile Leu Pro Gly Thr Pro 
        835                 840                 845             


Leu Ser Arg Ala Asp Leu Arg Ala Val Asn Ile Asn Leu Cys Asp Met 
    850                 855                 860                 


Cys Val Ile Leu Ser Ala Asn Gln Asn Asn Ile Asp Asp Thr Ser Leu 
865                 870                 875                 880 


Gln Asp Lys Glu Cys Ile Leu Ala Ser Leu Asn Ile Lys Ser Met Gln 
                885                 890                 895     


Phe Asp Asp Ser Ile Gly Val Leu Gln Ala Asn Ser Gln Gly Phe Thr 
            900                 905                 910         


Pro Pro Gly Met Asp Arg Ser Ser Pro Asp Asn Ser Pro Val His Gly 
        915                 920                 925             


Met Leu Arg Gln Pro Ser Ile Thr Thr Gly Val Asn Ile Pro Ile Ile 
    930                 935                 940                 


Thr Glu Leu Val Asn Asp Thr Asn Val Gln Phe Leu Asp Gln Asp Asp 
945                 950                 955                 960 


Asp Asp Asp Pro Asp Thr Glu Leu Tyr Leu Thr Gln Pro Phe Ala Cys 
                965                 970                 975     


Gly Thr Ala Phe Ala Val Ser Val Leu Asp Ser Leu Met Ser Ala Thr 
            980                 985                 990         


Tyr Phe Asn Asp Asn Ile Leu Thr  Leu Ile Arg Thr Leu  Val Thr Gly 
        995                 1000                 1005             


Gly Ala  Thr Pro Glu Leu Glu  Ala Leu Ile Ala Glu  Glu Asn Ala 
    1010                 1015                 1020             


Leu Arg  Gly Gly Tyr Ser Thr  Pro Gln Thr Leu Ala  Asn Arg Asp 
    1025                 1030                 1035             


Arg Cys  Arg Val Ala Gln Leu  Ala Leu Leu Asp Gly  Pro Phe Ala 
    1040                 1045                 1050             


Asp Leu  Gly Asp Gly Gly Cys  Tyr Gly Asp Leu Phe  Cys Lys Ala 
    1055                 1060                 1065             


Leu Lys  Thr Tyr Asn Met Leu  Cys Phe Gly Ile Tyr  Arg Leu Arg 
    1070                 1075                 1080             


Asp Ala  His Leu Ser Thr Pro  Ser Gln Cys Thr Lys  Arg Tyr Val 
    1085                 1090                 1095             


Ile Thr  Asn Pro Pro Tyr Glu  Phe Glu Leu Val Pro  Thr Asp Leu 
    1100                 1105                 1110             


Ile Phe  Cys Leu Met Gln Phe  Asp His Asn Ala Gly  Gln Ser Arg 
    1115                 1120                 1125             


Ala Ser  Leu Ser His Ser Ser  His Ser Ser Gln Ser  Ser Ser Lys 
    1130                 1135                 1140             


Lys Ser  Ser Ser Val His Ser  Ile Pro Ser Thr Ala  Asn Arg Gln 
    1145                 1150                 1155             


Asn Arg  Pro Lys Ser Arg Glu  Ser Arg Asp Lys Gln  Lys Tyr Val 
    1160                 1165                 1170             


Gln Glu  Glu Arg Leu 
    1175             


<210>  9
<211>  1419
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SM22alpha promoter sequence

<400>  9
gaattcagga cgtaatcagt ggctggaaag caagagctct agaggagctc tgacccttcc       60

ttcagatgcc acaaggaggt gctggagttc tatgcaccaa cagccaggct ggctgtagtg      120

gattgagcgt ctgaggctgc acctctctgg gttctgggtg agactgaccc tgcctgaggg      180

ttctctcctt ccctctctct ccctctccct ctccctctct ctgtttcctg aggtttccag      240

gattggggat gacaccacta aagccttacc ttttaagaag ttgcattcag tgagtgtgtg      300

cagatagggg cagaggagag ctggttctgt ctccactgtg tttggtcttg tcagaccatc      360

aggtgtgata gcagttgtct ttaaccctaa ccctgagcct tcccttccca agaccactga      420

agctaggtgc aagataagtg gggacccttt aggatctttc acgataagga ctattttgaa      480

gggagggagg gtgacactgt ttaccctagt gtctccagcc ttgccaggcc ttaaacatcc      540

gcccattgtc aaggggccag ggttgacttg ctgctaaaca aggcactccc tagagaagca      600

gcataccata cctgtgggca ggatgaccca tgttctgcca cgcacttggt aggccacttt      660

gaacctcaat tttctcaact gttaaatggg gtggtaactg ataaagggga acgtgaaagg      720

aaggcgtttg catagtgcct ggttgtgcag gtcaagacta gttcccacca actcgatttt      780

aaagccttgc aagaaggtgg ccttgcaggt tcctttgtcg ggccaaactc tagaatgcct      840

ccccctttct agagcagacc caagtccggg taacaaggaa gggtttcagg gtcctgccca      900

ttcccggccg ccctcagcac cgccccgccc cgacccccgc agcatctcca cagcttatta      960

tagcttaaac cctgcagcca actcctttct gggactcaga agacatagca ggtactgaac     1020

gtctcacctg ctgaggtggt cctagtcctc acccgctcta gcccgctaga agccttggaa     1080

ctatctcata ccaggctgca cttgtttgtc ttctcattga taaaaggttt aagcatgcag     1140

agaatgtctc cggctgcccc cgacagactg ctccaacttg gtgtctttcc ccaaatatgg     1200

agcctgtgtg gagtgagtgg ggcggcccgg ggtggtgagc caagcagact tccatgggca     1260

gggaggggcg ccagcggacg gcagaggggt gacatcactg cctaggcggc ctttaaaccc     1320

ctcacccagc cggcgcccca gcccgtctgc cccagcccag acaccgaagc tactctcctt     1380

ccagtccaca aacgaccaag ccttgtaagt gcaagtcat                            1419


<210>  10
<211>  2999
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVAX vector

<400>  10
gactcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta       60

atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata      120

acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat      180

aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga      240

ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc      300

ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt      360

atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat      420

gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag      480

tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc      540

aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga      600

ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga      660

aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt      720

accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc      780

ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta      840

gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca      900

ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc      960

attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata     1020

gcaggcatgc tggggatgcg gtgggctcta tggcttctac tgggcggttt tatggacagc     1080

aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt     1140

aaactggatg gctttctcgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca     1200

agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc     1260

ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc     1320

tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga     1380

cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac     1440

gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct     1500

gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa     1560

agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc     1620

attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct     1680

tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc     1740

caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg     1800

cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct     1860

gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct     1920

tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca     1980

gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attattaacg cttacaattt     2040

cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacaggtggc     2100

acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat     2160

atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatagca cgtgctaaaa     2220

cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa     2280

atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga     2340

tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg     2400

ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact     2460

ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac     2520

cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg     2580

gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg     2640

gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga     2700

acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc     2760

gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg     2820

agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc     2880

tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc     2940

agcaacgcgg cctttttacg gttcctgggc ttttgctggc cttttgctca catgttctt      2999


<210>  11
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated Slo sequence

<400>  11
atggtcacaa tgtcctccgt tggttatggg gat                                    33


