                         SEQUENCE LISTING

<110>  Her Majesty the Queen in Right of Canada, as represented by
        the Minister of Agriculture and Agri-Food
 
<120>  VACCINE AGAINST NECROTIC ENTERITIS IN POULTRY

<130>  3688-157

<150>  US 62/513,001
<151>  2017-05-31

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  2091
<212>  DNA
<213>  Clostridium perfringens

<400>  1
atgaaaataa ataaaaaaat ttttagcatg ctatttatgg ttattgtact ttttacatgc       60

atatcatcaa atttttctgt ttctgcttct tctattcaaa gaggaagaga tatcagtaat      120

gaggtagtta caagcctagt ggctactcca aatagtataa atgatggtgg aaacgttcag      180

gttcgtttgg aatttaaaga aaatcatcaa agaaatatac aaagtggaga tactataact      240

gtcaaatgga caaattcagg ggaagtattt tttgaaggat atgaaaaaac aattccactt      300

tatataaaag accaaaatgt tggtcaagca gtaatagaga aaacaggtgc aacacttaca      360

tttaatgata aaatagataa attagatgat gttggtggat gggcaacatt tactttgcaa      420

ggaagaaaca ttacctcagg taatcatgaa cacacaggaa tagcatatat tatatctggt      480

tcaaagcggg cagatgtaaa tataaccaaa ccagaatcag gtacaactag tgtattctat      540

tataaaacag gtagtatgta taccaatgat acaaatcatg tcaattggtg gttactggtg      600

aatccaagca aggtatattc tgaaaaaaac gtttatattc aagatgaaat ccaaggcgga      660

caaacattag aacctgattc ttttgaaata gtagtaactt ggtatgatgg ttatgtagaa      720

aagtttaaag gaaaagaagc gataagggaa ttccataata aatatccaaa ttcaaatata      780

tcggtatcag aaaataaaat aacagtaaac atttcacaag aggattccac acaaaagttt      840

attaatattt tttataaaac taagattaca aatccgaaac aaaaagaatt cgttaataat      900

acaaaagcat ggtttaaaga gtataataag ccagctgtaa atggagaatc ctttaaccat      960

agcgtacaaa atattaatgc agatgctgga gttaatggaa ctgtaaaagg cgaattaaaa     1020

atcataaaaa cattaaaaga taaaagtatt ccaattaaag atgttcagtt taagatgaga     1080

agagttgata atacagttat caaagatggt aaaaaagaat tattactaac aactgatgat     1140

aaaggtattg caaatgtaaa aggtcttcct gtaggaaaat atgaagtaaa agagatttca     1200

gctccagaat ggattgcttt taatcctctt attgcaccaa aattggaatt cacaatatca     1260

gatcaggaca cagaaggcaa attgtgggct gttgaaaatg aattaaagac aatttcaatt     1320

ccggttgaaa aggtctgggt aggacaaact agtgaacgag cagaaatcaa gctttttgca     1380

gatggtattg aagtagacaa agtgatttta aatgcagata acaattggaa acacacattt     1440

gaaaataaac ctgaatataa ttcagaaaca aaacagaaaa tcaattattc tgtgtcagag     1500

acaactattt ctggatatga aagcaatatc acaggcgatg ctaagaatgg ttttattgta     1560

accaatacag aacttcctga tttgactatt ggtaaagaag ttataggaga attgggtgac     1620

aagacgaagg tatttaactt tgagcttaca ttaaagcaag cagatggaaa gcctatcaat     1680

ggtaaattta attacattgg tagtgtagat gacaggtaca aaaaagaaag cataaagcct     1740

tctgatggtg agattacttt tatagaagga aaagcaacta taactttatc acatggacaa     1800

gagattacaa tcaaggattt accatatggg gttacatata aagttatgga aaaagaagct     1860

aatgaaaatg gctatttaac tacctataat ggaaataacg aagtcacaac aggtgaattg     1920

aaacaggata caaaagtaca ggtagttaac aacaaagagt ttgttccaac aactggtata     1980

tcaaccacaa cagagcaagg tacaatggtt ggaatggtga ttttttctat aggaatactt     2040

atggtcatga ttgtagttct tttacaattg aataaaggac tgaaaagatg a              2091


<210>  2
<211>  1074
<212>  DNA
<213>  Clostridium perfringens

<400>  2
atgataaaca agaaaaaatt aagtgcatta ttattaagtg gagcaatgtt tatgagtatg       60

aatacaaatg tattcgcatc aaatttacct tctggagggg tagaaggtac agaacagaat      120

cctgcaaaag caacaattac aaagaatttt gaatttccag aaggtattaa tacacctagt      180

gcaacattca agtttacagc agaaaaaata actaatgatg cgccagatgc aacaattgga      240

gatattaatt atacacaagg ggataatgga actttatcaa atggaaaata tagtgtaaag      300

aaaacaactg aaattacttt tggaaatttc ccacatgcag gagaatatga ttataatgta      360

aaagaaacga atgagggagt aggtggtatt acatatgata caaaagaata caaagttcat      420

gtgtatgttg caaatagtaa cgctatggat ggaaaaactt atgtaaaagc cattacatca      480

gaaaatggag gtgaaaaagc tccaattgag tttgttaata catataaaaa ggacacttct      540

ttacttatag aaaagaatgt aataggagat ttagctgact taacaaaaca gtttgagttt      600

cagattaatt taaaaaaatc agcaacatct gacataacaa aattcgaagg aaatattatt      660

agaaaagatg gtaaaataga gcctgtaaca tatacagctg aaaatacaga aacttttaaa      720

ttagcaaatg gagataaact taagtttgaa agtattccag caggaacaaa atatgaagta      780

aaagagatag gtgctagtga tggatataca ccttctataa cagtaattga aaatggaaat      840

gagacttcta ataatcgtac ggtagctgaa aaagatggta tatcatctaa gtcaaattct      900

aatgataact taattggtga aggtgaaaac aaagtaacat ttacaaacac atataatgac      960

aaacctatca caggtattgt tatgaataat attccattta ttctaatgat tagttttgct     1020

gtccttggat ttggtgcttt agctattata aaaagacgta aaactataag ataa           1074


<210>  3
<211>  660
<212>  DNA
<213>  Clostridium perfringens

<400>  3
atggaaacaa agaaaataag aaacaaaatc cttatggcta tcgtagcatt gagctttata       60

ttgcttccaa acactagagt atatgctact gaaaatacag caaatattcc tttgatagtt      120

agacaggaat ttaatgtata tacgaaagat tcaaaagcaa tagacatgat tggaaaatac      180

gagctaaagg caataagtga aaatgcccct atgccagaag aaagtaaaaa tggaagtttt      240

atctttaata tagatggaaa tgataagcag tttactattc cattagctta tacacatggt      300

ggtgtgtata tctatcaaat tcaacagata acgcaatcta aagataatta catatatgat      360

aaaaatagct ataagataac tgtatatgta aaaaatgcag aaaataatca tttaatacca      420

caaattattg tgaaaaatga aaataatgaa aaatgtgaag aaatatgttt ttataacatt      480

tacaaacaaa aaaataaaat taatgagatt tctaaaacac catataagcc aaatggaata      540

aatgttccta aaacaggcga taccacaaac attggatttt atattgtaat acttataatt      600

tcacttggat tacttgtggt attgaaatgg aaagaatata aaaagagaaa aaaagaataa      660


<210>  4
<211>  2088
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cnaA codon optimized

<400>  4
atgaagatca acaagaagat cttcagcatg ttatttatgg tcattgtgct gttcacctgt       60

atcagctcta acttcagtgt gagcgcgtca agcatccagc gcggccggga catcagcaac      120

gaggtggtga catcgctcgt agctaccccg aatagcatca acgatggtgg taacgtccaa      180

gtgcgtctgg aatttaaaga gaatcaccag cggaacattc agtccggcga cacgattacg      240

gtcaaatgga ctaactcagg tgaggtcttt tttgaaggct acgaaaaaac catcccgctg      300

tatatcaagg atcagaacgt tggccaggcg gttattgaaa aaaccggtgc aacattaaca      360

ttcaacgata agatcgacaa attagatgat gtcggcggct gggccacatt cacgctccag      420

ggtcgcaata ttacttcagg aaatcatgag catactggta ttgcgtacat tatctcgggt      480

agcaaacgtg cggacgttaa catcacaaaa cctgaatccg gaacaacgtc tgtgttttac      540

tacaagacgg gttcgatgta caccaatgac acaaatcatg tgaattggtg gctgctggtt      600

aacccgagca aagtatactc tgagaaaaat gtctatattc aggatgaaat tcaaggcggt      660

cagaccctgg agccggacag ttttgaaatc gtcgttacat ggtacgatgg ttatgtggaa      720

aaatttaaag gtaaagaagc gatccgggag ttccacaata aatatccgaa tagtaatatc      780

tcggtcagtg aaaataaaat cacggtaaat atttcgcaag aagattccac ccaaaaattc      840

attaacatct tttacaagac taaaatcacc aacccgaagc agaaagaatt tgtaaacaac      900

accaaagcct ggttcaaaga gtacaataag ccggcggtta acggtgaaag ttttaatcac      960

agtgtgcaga atatcaacgc agatgccggg gtaaatggta ctgttaaagg tgaattgaaa     1020

attatcaaaa ccctgaaaga taaaagtatt ccgatcaagg atgtgcagtt taagatgcgc     1080

cgcgtggata ataccgttat taaagacggc aagaaagagc tgctgttgac cacagatgat     1140

aaagggattg caaacgtgaa aggtctgcca gtcgggaaat acgaagtcaa agaaatcagt     1200

gcgcctgagt ggatcgcctt caatccactg attgcgccca aacttgaatt tacgatcagc     1260

gatcaagaca cagaggggaa attatgggca gtggaaaacg aactcaaaac catctcgatt     1320

ccggtcgaaa aagtctgggt aggtcagacg agtgaacggg cggagatcaa actgtttgcg     1380

gatggaattg aagttgataa ggtgatcctg aacgcggata ataattggaa gcacaccttt     1440

gagaataaac ccgaatataa ctccgagact aaacaaaaaa tcaactatag tgtgagcgaa     1500

actaccatca gtggctatga atcaaatatt actggcgatg cgaaaaacgg atttattgtc     1560

accaacacag aactgcctga tttgacgatc gggaaagagg taatcggcga actcggcgat     1620

aaaaccaagg tattcaactt tgaactgaca cttaagcagg ctgacggaaa gcccattaac     1680

gggaaattta actatattgg ttcggtggat gatcgttata agaaggaatc gattaagcct     1740

agcgatgggg aaattacgtt catcgaggga aaagcaacga ttaccctctc ccacggacaa     1800

gagatcacca ttaaggacct tccgtatggt gtgacctata aagtcatgga aaaagaagcc     1860

aacgagaatg gatatttaac cacttacaac ggaaataacg aagtcaccac cggggagttg     1920

aaacaggata cgaaagtaca agtggttaat aataaagaat tcgtcccgac aaccgggatc     1980

agcaccacca ccgaacaggg aaccatggtc gggatggtga tctttagcat cggtattctc     2040

atggtaatga ttgtcgttct gctgcagctg aataaaggac tgaaacgc                  2088


<210>  5
<211>  1086
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fimA codon optimized

<400>  5
atgattaata aaaagaaact gtcggcgctg ctcttaagcg gggccatgtt tatgagcatg       60

aacacgaatg tgttcgcgtc taacctccca tcgggtggtg tggagggcac cgaacaaaac      120

ccagcgaaag cgacaatcac gaaaaacttc gagtttccgg aaggtattaa tacacccagc      180

gcgacattca aatttaccgc cgaaaaaatt accaacgatg cgccggatgc tactattggc      240

gacatcaatt atacccaagg tgataatggg acgttaagca atggcaaata cagtgtgaaa      300

aagactaccg agattacctt cgggaacttc ccgcatgctg gtgagtatga ttataacgtc      360

aaagaaacca atgaaggcgt gggtggcatt acttacgata cgaaagaata taaagttcat      420

gtgtatgtgg ccaactcaaa tgcgatggac ggtaagacat atgttaaagc gattactagc      480

gaaaatggcg gggaaaaagc accgatcgaa ttcgttaaca cctataaaaa agatacgtcg      540

ttactgattg aaaaaaatgt aattggcgat ctggcagacc tcaccaaaca gtttgagttt      600

caaatcaact tgaaaaagag cgcgactagt gatattacca agtttgaagg taacattatt      660

cgcaaagacg gtaagattga acccgtgacc tataccgcgg aaaataccga gacctttaag      720

ttagccaacg gagacaagtt aaaattcgag tccatccccg ccggtacaaa atatgaagtc      780

aaggaaatcg gggcgagcga tgggtacacg ccctcaatca ccgttatcga aaatggcaac      840

gaaacctcaa ataaccgcac tgtagccgaa aaagatggaa tctctagcaa aagcaactcg      900

aacgacaatt taatcggcga aggcgaaaat aaagtgacct ttaccaatac gtacaacgat      960

aaaccaatca cgggaatcgt aatgaataat attccgttca ttcttatgat tagctttgcc     1020

gttcttggct tcggtgcatt agcgatcatt aaacgccgca aaaccatccg ccccatcgat     1080

acgcgt                                                                1086


<210>  6
<211>  624
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fimB codon optimized

<400>  6
atggctattg ttgctttgtc atttatcctg ctcccgaata cccgggtcta tgcgacggag       60

aacaccgcta atatcccgtt aattgtacgc caagaattta atgtttacac taaagattct      120

aaagccattg acatgatcgg aaaatatgaa ttaaaagcca tttctgagaa cgctcccatg      180

ccggaggaat caaaaaatgg tagctttatt tttaacatcg acggtaatga taaacagttt      240

actattccgc tggcgtacac tcacggtggc gtctacatct atcaaatcca gcaaattacc      300

cagagcaagg ataactacat ctacgataaa aacagctata aaatcacggt atatgtcaag      360

aacgcagaaa acaatcatct gatcccgcag attattgtaa aaaatgagaa caatgaaaaa      420

tgtgaagaaa tctgcttcta caatatctac aaacagaaaa acaagatcaa tgagatctct      480

aaaaccccct ataagccgaa tggtattaat gtcccgaaaa cgggtgatac cacgaacatc      540

ggattctaca ttgtgatctt gattatttcc ctgggcctgc tggtggtctt gaagtggaaa      600

gaatataaaa aacgtaagaa ggaa                                             624


<210>  7
<211>  1887
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cnaA truncated

<400>  7
tcaagcatcc agcgcggccg ggacatcagc aacgaggtgg tgacatcgct cgtagctacc       60

ccgaatagca tcaacgatgg tggtaacgtc caagtgcgtc tggaatttaa agagaatcac      120

cagcggaaca ttcagtccgg cgacacgatt acggtcaaat ggactaactc aggtgaggtc      180

ttttttgaag gctacgaaaa aaccatcccg ctgtatatca aggatcagaa cgttggccag      240

gcggttattg aaaaaaccgg tgcaacatta acattcaacg ataagatcga caaattagat      300

gatgtcggcg gctgggccac attcacgctc cagggtcgca atattacttc aggaaatcat      360

gagcatactg gtattgcgta cattatctcg ggtagcaaac gtgcggacgt taacatcaca      420

aaacctgaat ccggaacaac gtctgtgttt tactacaaga cgggttcgat gtacaccaat      480

gacacaaatc atgtgaattg gtggctgctg gttaacccga gcaaagtata ctctgagaaa      540

aatgtctata ttcaggatga aattcaaggc ggtcagaccc tggagccgga cagttttgaa      600

atcgtcgtta catggtacga tggttatgtg gaaaaattta aaggtaaaga agcgatccgg      660

gagttccaca ataaatatcc gaatagtaat atctcggtca gtgaaaataa aatcacggta      720

aatatttcgc aagaagattc cacccaaaaa ttcattaaca tcttttacaa gactaaaatc      780

accaacccga agcagaaaga atttgtaaac aacaccaaag cctggttcaa agagtacaat      840

aagccggcgg ttaacggtga aagttttaat cacagtgtgc agaatatcaa cgcagatgcc      900

ggggtaaatg gtactgttaa aggtgaattg aaaattatca aaaccctgaa agataaaagt      960

attccgatca aggatgtgca gtttaagatg cgccgcgtgg ataataccgt tattaaagac     1020

ggcaagaaag agctgctgtt gaccacagat gataaaggga ttgcaaacgt gaaaggtctg     1080

ccagtcggga aatacgaagt caaagaaatc agtgcgcctg agtggatcgc cttcaatcca     1140

ctgattgcgc ccaaacttga atttacgatc agcgatcaag acacagaggg gaaattatgg     1200

gcagtggaaa acgaactcaa aaccatctcg attccggtcg aaaaagtctg ggtaggtcag     1260

acgagtgaac gggcggagat caaactgttt gcggatggaa ttgaagttga taaggtgatc     1320

ctgaacgcgg ataataattg gaagcacacc tttgagaata aacccgaata taactccgag     1380

actaaacaaa aaatcaacta tagtgtgagc gaaactacca tcagtggcta tgaatcaaat     1440

attactggcg atgcgaaaaa cggatttatt gtcaccaaca cagaactgcc tgatttgacg     1500

atcgggaaag aggtaatcgg cgaactcggc gataaaacca aggtattcaa ctttgaactg     1560

acacttaagc aggctgacgg aaagcccatt aacgggaaat ttaactatat tggttcggtg     1620

gatgatcgtt ataagaagga atcgattaag cctagcgatg gggaaattac gttcatcgag     1680

ggaaaagcaa cgattaccct ctcccacgga caagagatca ccattaagga ccttccgtat     1740

ggtgtgacct ataaagtcat ggaaaaagaa gccaacgaga atggatattt aaccacttac     1800

aacggaaata acgaagtcac caccggggag ttgaaacagg atacgaaagt acaagtggtt     1860

aataataaag aattcgtccc gacaacc                                         1887


<210>  8
<211>  894
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fimA truncated

<400>  8
tctaacctcc catcgggtgg tgtggagggc accgaacaaa acccagcgaa agcgacaatc       60

acgaaaaact tcgagtttcc ggaaggtatt aatacaccca gcgcgacatt caaatttacc      120

gccgaaaaaa ttaccaacga tgcgccggat gctactattg gcgacatcaa ttatacccaa      180

ggtgataatg ggacgttaag caatggcaaa tacagtgtga aaaagactac cgagattacc      240

ttcgggaact tcccgcatgc tggtgagtat gattataacg tcaaagaaac caatgaaggc      300

gtgggtggca ttacttacga tacgaaagaa tataaagttc atgtgtatgt ggccaactca      360

aatgcgatgg acggtaagac atatgttaaa gcgattacta gcgaaaatgg cggggaaaaa      420

gcaccgatcg aattcgttaa cacctataaa aaagatacgt cgttactgat tgaaaaaaat      480

gtaattggcg atctggcaga cctcaccaaa cagtttgagt ttcaaatcaa cttgaaaaag      540

agcgcgacta gtgatattac caagtttgaa ggtaacatta ttcgcaaaga cggtaagatt      600

gaacccgtga cctataccgc ggaaaatacc gagaccttta agttagccaa cggagacaag      660

ttaaaattcg agtccatccc cgccggtaca aaatatgaag tcaaggaaat cggggcgagc      720

gatgggtaca cgccctcaat caccgttatc gaaaatggca acgaaacctc aaataaccgc      780

actgtagccg aaaaagatgg aatctctagc aaaagcaact cgaacgacaa tttaatcggc      840

gaaggcgaaa ataaagtgac ctttaccaat acgtacaacg ataaaccaat cacg            894


<210>  9
<211>  468
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fimB truncated

<400>  9
acggagaaca ccgctaatat cccgttaatt gtacgccaag aatttaatgt ttacactaaa       60

gattctaaag ccattgacat gatcggaaaa tatgaattaa aagccatttc tgagaacgct      120

cccatgccgg aggaatcaaa aaatggtagc tttattttta acatcgacgg taatgataaa      180

cagtttacta ttccgctggc gtacactcac ggtggcgtct acatctatca aatccagcaa      240

attacccaga gcaaggataa ctacatctac gataaaaaca gctataaaat cacggtatat      300

gtcaagaacg cagaaaacaa tcatctgatc ccgcagatta ttgtaaaaaa tgagaacaat      360

gaaaaatgtg aagaaatctg cttctacaat atctacaaac agaaaaacaa gatcaatgag      420

atctctaaaa ccccctataa gccgaatggt attaatgtcc cgaaaacg                   468


<210>  10
<211>  696
<212>  PRT
<213>  Clostridium perfringens

<400>  10

Met Lys Ile Asn Lys Lys Ile Phe Ser Met Leu Phe Met Val Ile Val 
1               5                   10                  15      


Leu Phe Thr Cys Ile Ser Ser Asn Phe Ser Val Ser Ala Ser Ser Ile 
            20                  25                  30          


Gln Arg Gly Arg Asp Ile Ser Asn Glu Val Val Thr Ser Leu Val Ala 
        35                  40                  45              


Thr Pro Asn Ser Ile Asn Asp Gly Gly Asn Val Gln Val Arg Leu Glu 
    50                  55                  60                  


Phe Lys Glu Asn His Gln Arg Asn Ile Gln Ser Gly Asp Thr Ile Thr 
65                  70                  75                  80  


Val Lys Trp Thr Asn Ser Gly Glu Val Phe Phe Glu Gly Tyr Glu Lys 
                85                  90                  95      


Thr Ile Pro Leu Tyr Ile Lys Asp Gln Asn Val Gly Gln Ala Val Ile 
            100                 105                 110         


Glu Lys Thr Gly Ala Thr Leu Thr Phe Asn Asp Lys Ile Asp Lys Leu 
        115                 120                 125             


Asp Asp Val Gly Gly Trp Ala Thr Phe Thr Leu Gln Gly Arg Asn Ile 
    130                 135                 140                 


Thr Ser Gly Asn His Glu His Thr Gly Ile Ala Tyr Ile Ile Ser Gly 
145                 150                 155                 160 


Ser Lys Arg Ala Asp Val Asn Ile Thr Lys Pro Glu Ser Gly Thr Thr 
                165                 170                 175     


Ser Val Phe Tyr Tyr Lys Thr Gly Ser Met Tyr Thr Asn Asp Thr Asn 
            180                 185                 190         


His Val Asn Trp Trp Leu Leu Val Asn Pro Ser Lys Val Tyr Ser Glu 
        195                 200                 205             


Lys Asn Val Tyr Ile Gln Asp Glu Ile Gln Gly Gly Gln Thr Leu Glu 
    210                 215                 220                 


Pro Asp Ser Phe Glu Ile Val Val Thr Trp Tyr Asp Gly Tyr Val Glu 
225                 230                 235                 240 


Lys Phe Lys Gly Lys Glu Ala Ile Arg Glu Phe His Asn Lys Tyr Pro 
                245                 250                 255     


Asn Ser Asn Ile Ser Val Ser Glu Asn Lys Ile Thr Val Asn Ile Ser 
            260                 265                 270         


Gln Glu Asp Ser Thr Gln Lys Phe Ile Asn Ile Phe Tyr Lys Thr Lys 
        275                 280                 285             


Ile Thr Asn Pro Lys Gln Lys Glu Phe Val Asn Asn Thr Lys Ala Trp 
    290                 295                 300                 


Phe Lys Glu Tyr Asn Lys Pro Ala Val Asn Gly Glu Ser Phe Asn His 
305                 310                 315                 320 


Ser Val Gln Asn Ile Asn Ala Asp Ala Gly Val Asn Gly Thr Val Lys 
                325                 330                 335     


Gly Glu Leu Lys Ile Ile Lys Thr Leu Lys Asp Lys Ser Ile Pro Ile 
            340                 345                 350         


Lys Asp Val Gln Phe Lys Met Arg Arg Val Asp Asn Thr Val Ile Lys 
        355                 360                 365             


Asp Gly Lys Lys Glu Leu Leu Leu Thr Thr Asp Asp Lys Gly Ile Ala 
    370                 375                 380                 


Asn Val Lys Gly Leu Pro Val Gly Lys Tyr Glu Val Lys Glu Ile Ser 
385                 390                 395                 400 


Ala Pro Glu Trp Ile Ala Phe Asn Pro Leu Ile Ala Pro Lys Leu Glu 
                405                 410                 415     


Phe Thr Ile Ser Asp Gln Asp Thr Glu Gly Lys Leu Trp Ala Val Glu 
            420                 425                 430         


Asn Glu Leu Lys Thr Ile Ser Ile Pro Val Glu Lys Val Trp Val Gly 
        435                 440                 445             


Gln Thr Ser Glu Arg Ala Glu Ile Lys Leu Phe Ala Asp Gly Ile Glu 
    450                 455                 460                 


Val Asp Lys Val Ile Leu Asn Ala Asp Asn Asn Trp Lys His Thr Phe 
465                 470                 475                 480 


Glu Asn Lys Pro Glu Tyr Asn Ser Glu Thr Lys Gln Lys Ile Asn Tyr 
                485                 490                 495     


Ser Val Ser Glu Thr Thr Ile Ser Gly Tyr Glu Ser Asn Ile Thr Gly 
            500                 505                 510         


Asp Ala Lys Asn Gly Phe Ile Val Thr Asn Thr Glu Leu Pro Asp Leu 
        515                 520                 525             


Thr Ile Gly Lys Glu Val Ile Gly Glu Leu Gly Asp Lys Thr Lys Val 
    530                 535                 540                 


Phe Asn Phe Glu Leu Thr Leu Lys Gln Ala Asp Gly Lys Pro Ile Asn 
545                 550                 555                 560 


Gly Lys Phe Asn Tyr Ile Gly Ser Val Asp Asp Arg Tyr Lys Lys Glu 
                565                 570                 575     


Ser Ile Lys Pro Ser Asp Gly Glu Ile Thr Phe Ile Glu Gly Lys Ala 
            580                 585                 590         


Thr Ile Thr Leu Ser His Gly Gln Glu Ile Thr Ile Lys Asp Leu Pro 
        595                 600                 605             


Tyr Gly Val Thr Tyr Lys Val Met Glu Lys Glu Ala Asn Glu Asn Gly 
    610                 615                 620                 


Tyr Leu Thr Thr Tyr Asn Gly Asn Asn Glu Val Thr Thr Gly Glu Leu 
625                 630                 635                 640 


Lys Gln Asp Thr Lys Val Gln Val Val Asn Asn Lys Glu Phe Val Pro 
                645                 650                 655     


Thr Thr Gly Ile Ser Thr Thr Thr Glu Gln Gly Thr Met Val Gly Met 
            660                 665                 670         


Val Ile Phe Ser Ile Gly Ile Leu Met Val Met Ile Val Val Leu Leu 
        675                 680                 685             


Gln Leu Asn Lys Gly Leu Lys Arg 
    690                 695     


<210>  11
<211>  357
<212>  PRT
<213>  Clostridium perfringens

<400>  11

Met Ile Asn Lys Lys Lys Leu Ser Ala Leu Leu Leu Ser Gly Ala Met 
1               5                   10                  15      


Phe Met Ser Met Asn Thr Asn Val Phe Ala Ser Asn Leu Pro Ser Gly 
            20                  25                  30          


Gly Val Glu Gly Thr Glu Gln Asn Pro Ala Lys Ala Thr Ile Thr Lys 
        35                  40                  45              


Asn Phe Glu Phe Pro Glu Gly Ile Asn Thr Pro Ser Ala Thr Phe Lys 
    50                  55                  60                  


Phe Thr Ala Glu Lys Ile Thr Asn Asp Ala Pro Asp Ala Thr Ile Gly 
65                  70                  75                  80  


Asp Ile Asn Tyr Thr Gln Gly Asp Asn Gly Thr Leu Ser Asn Gly Lys 
                85                  90                  95      


Tyr Ser Val Lys Lys Thr Thr Glu Ile Thr Phe Gly Asn Phe Pro His 
            100                 105                 110         


Ala Gly Glu Tyr Asp Tyr Asn Val Lys Glu Thr Asn Glu Gly Val Gly 
        115                 120                 125             


Gly Ile Thr Tyr Asp Thr Lys Glu Tyr Lys Val His Val Tyr Val Ala 
    130                 135                 140                 


Asn Ser Asn Ala Met Asp Gly Lys Thr Tyr Val Lys Ala Ile Thr Ser 
145                 150                 155                 160 


Glu Asn Gly Gly Glu Lys Ala Pro Ile Glu Phe Val Asn Thr Tyr Lys 
                165                 170                 175     


Lys Asp Thr Ser Leu Leu Ile Glu Lys Asn Val Ile Gly Asp Leu Ala 
            180                 185                 190         


Asp Leu Thr Lys Gln Phe Glu Phe Gln Ile Asn Leu Lys Lys Ser Ala 
        195                 200                 205             


Thr Ser Asp Ile Thr Lys Phe Glu Gly Asn Ile Ile Arg Lys Asp Gly 
    210                 215                 220                 


Lys Ile Glu Pro Val Thr Tyr Thr Ala Glu Asn Thr Glu Thr Phe Lys 
225                 230                 235                 240 


Leu Ala Asn Gly Asp Lys Leu Lys Phe Glu Ser Ile Pro Ala Gly Thr 
                245                 250                 255     


Lys Tyr Glu Val Lys Glu Ile Gly Ala Ser Asp Gly Tyr Thr Pro Ser 
            260                 265                 270         


Ile Thr Val Ile Glu Asn Gly Asn Glu Thr Ser Asn Asn Arg Thr Val 
        275                 280                 285             


Ala Glu Lys Asp Gly Ile Ser Ser Lys Ser Asn Ser Asn Asp Asn Leu 
    290                 295                 300                 


Ile Gly Glu Gly Glu Asn Lys Val Thr Phe Thr Asn Thr Tyr Asn Asp 
305                 310                 315                 320 


Lys Pro Ile Thr Gly Ile Val Met Asn Asn Ile Pro Phe Ile Leu Met 
                325                 330                 335     


Ile Ser Phe Ala Val Leu Gly Phe Gly Ala Leu Ala Ile Ile Lys Arg 
            340                 345                 350         


Arg Lys Thr Ile Arg 
        355         


<210>  12
<211>  219
<212>  PRT
<213>  Clostridium perfringens

<400>  12

Met Glu Thr Lys Lys Ile Arg Asn Lys Ile Leu Met Ala Ile Val Ala 
1               5                   10                  15      


Leu Ser Phe Ile Leu Leu Pro Asn Thr Arg Val Tyr Ala Thr Glu Asn 
            20                  25                  30          


Thr Ala Asn Ile Pro Leu Ile Val Arg Gln Glu Phe Asn Val Tyr Thr 
        35                  40                  45              


Lys Asp Ser Lys Ala Ile Asp Met Ile Gly Lys Tyr Glu Leu Lys Ala 
    50                  55                  60                  


Ile Ser Glu Asn Ala Pro Met Pro Glu Glu Ser Lys Asn Gly Ser Phe 
65                  70                  75                  80  


Ile Phe Asn Ile Asp Gly Asn Asp Lys Gln Phe Thr Ile Pro Leu Ala 
                85                  90                  95      


Tyr Thr His Gly Gly Val Tyr Ile Tyr Gln Ile Gln Gln Ile Thr Gln 
            100                 105                 110         


Ser Lys Asp Asn Tyr Ile Tyr Asp Lys Asn Ser Tyr Lys Ile Thr Val 
        115                 120                 125             


Tyr Val Lys Asn Ala Glu Asn Asn His Leu Ile Pro Gln Ile Ile Val 
    130                 135                 140                 


Lys Asn Glu Asn Asn Glu Lys Cys Glu Glu Ile Cys Phe Tyr Asn Ile 
145                 150                 155                 160 


Tyr Lys Gln Lys Asn Lys Ile Asn Glu Ile Ser Lys Thr Pro Tyr Lys 
                165                 170                 175     


Pro Asn Gly Ile Asn Val Pro Lys Thr Gly Asp Thr Thr Asn Ile Gly 
            180                 185                 190         


Phe Tyr Ile Val Ile Leu Ile Ile Ser Leu Gly Leu Leu Val Val Leu 
        195                 200                 205             


Lys Trp Lys Glu Tyr Lys Lys Arg Lys Lys Glu 
    210                 215                 


<210>  13
<211>  680
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cnaA expressed His-tagged

<400>  13

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Arg 
            20                  25                  30          


Gly Ser Glu Phe Ser Ser Ile Gln Arg Gly Arg Asp Ile Ser Asn Glu 
        35                  40                  45              


Val Val Thr Ser Leu Val Ala Thr Pro Asn Ser Ile Asn Asp Gly Gly 
    50                  55                  60                  


Asn Val Gln Val Arg Leu Glu Phe Lys Glu Asn His Gln Arg Asn Ile 
65                  70                  75                  80  


Gln Ser Gly Asp Thr Ile Thr Val Lys Trp Thr Asn Ser Gly Glu Val 
                85                  90                  95      


Phe Phe Glu Gly Tyr Glu Lys Thr Ile Pro Leu Tyr Ile Lys Asp Gln 
            100                 105                 110         


Asn Val Gly Gln Ala Val Ile Glu Lys Thr Gly Ala Thr Leu Thr Phe 
        115                 120                 125             


Asn Asp Lys Ile Asp Lys Leu Asp Asp Val Gly Gly Trp Ala Thr Phe 
    130                 135                 140                 


Thr Leu Gln Gly Arg Asn Ile Thr Ser Gly Asn His Glu His Thr Gly 
145                 150                 155                 160 


Ile Ala Tyr Ile Ile Ser Gly Ser Lys Arg Ala Asp Val Asn Ile Thr 
                165                 170                 175     


Lys Pro Glu Ser Gly Thr Thr Ser Val Phe Tyr Tyr Lys Thr Gly Ser 
            180                 185                 190         


Met Tyr Thr Asn Asp Thr Asn His Val Asn Trp Trp Leu Leu Val Asn 
        195                 200                 205             


Pro Ser Lys Val Tyr Ser Glu Lys Asn Val Tyr Ile Gln Asp Glu Ile 
    210                 215                 220                 


Gln Gly Gly Gln Thr Leu Glu Pro Asp Ser Phe Glu Ile Val Val Thr 
225                 230                 235                 240 


Trp Tyr Asp Gly Tyr Val Glu Lys Phe Lys Gly Lys Glu Ala Ile Arg 
                245                 250                 255     


Glu Phe His Asn Lys Tyr Pro Asn Ser Asn Ile Ser Val Ser Glu Asn 
            260                 265                 270         


Lys Ile Thr Val Asn Ile Ser Gln Glu Asp Ser Thr Gln Lys Phe Ile 
        275                 280                 285             


Asn Ile Phe Tyr Lys Thr Lys Ile Thr Asn Pro Lys Gln Lys Glu Phe 
    290                 295                 300                 


Val Asn Asn Thr Lys Ala Trp Phe Lys Glu Tyr Asn Lys Pro Ala Val 
305                 310                 315                 320 


Asn Gly Glu Ser Phe Asn His Ser Val Gln Asn Ile Asn Ala Asp Ala 
                325                 330                 335     


Gly Val Asn Gly Thr Val Lys Gly Glu Leu Lys Ile Ile Lys Thr Leu 
            340                 345                 350         


Lys Asp Lys Ser Ile Pro Ile Lys Asp Val Gln Phe Lys Met Arg Arg 
        355                 360                 365             


Val Asp Asn Thr Val Ile Lys Asp Gly Lys Lys Glu Leu Leu Leu Thr 
    370                 375                 380                 


Thr Asp Asp Lys Gly Ile Ala Asn Val Lys Gly Leu Pro Val Gly Lys 
385                 390                 395                 400 


Tyr Glu Val Lys Glu Ile Ser Ala Pro Glu Trp Ile Ala Phe Asn Pro 
                405                 410                 415     


Leu Ile Ala Pro Lys Leu Glu Phe Thr Ile Ser Asp Gln Asp Thr Glu 
            420                 425                 430         


Gly Lys Leu Trp Ala Val Glu Asn Glu Leu Lys Thr Ile Ser Ile Pro 
        435                 440                 445             


Val Glu Lys Val Trp Val Gly Gln Thr Ser Glu Arg Ala Glu Ile Lys 
    450                 455                 460                 


Leu Phe Ala Asp Gly Ile Glu Val Asp Lys Val Ile Leu Asn Ala Asp 
465                 470                 475                 480 


Asn Asn Trp Lys His Thr Phe Glu Asn Lys Pro Glu Tyr Asn Ser Glu 
                485                 490                 495     


Thr Lys Gln Lys Ile Asn Tyr Ser Val Ser Glu Thr Thr Ile Ser Gly 
            500                 505                 510         


Tyr Glu Ser Asn Ile Thr Gly Asp Ala Lys Asn Gly Phe Ile Val Thr 
        515                 520                 525             


Asn Thr Glu Leu Pro Asp Leu Thr Ile Gly Lys Glu Val Ile Gly Glu 
    530                 535                 540                 


Leu Gly Asp Lys Thr Lys Val Phe Asn Phe Glu Leu Thr Leu Lys Gln 
545                 550                 555                 560 


Ala Asp Gly Lys Pro Ile Asn Gly Lys Phe Asn Tyr Ile Gly Ser Val 
                565                 570                 575     


Asp Asp Arg Tyr Lys Lys Glu Ser Ile Lys Pro Ser Asp Gly Glu Ile 
            580                 585                 590         


Thr Phe Ile Glu Gly Lys Ala Thr Ile Thr Leu Ser His Gly Gln Glu 
        595                 600                 605             


Ile Thr Ile Lys Asp Leu Pro Tyr Gly Val Thr Tyr Lys Val Met Glu 
    610                 615                 620                 


Lys Glu Ala Asn Glu Asn Gly Tyr Leu Thr Thr Tyr Asn Gly Asn Asn 
625                 630                 635                 640 


Glu Val Thr Thr Gly Glu Leu Lys Gln Asp Thr Lys Val Gln Val Val 
                645                 650                 655     


Asn Asn Lys Glu Phe Val Pro Thr Thr Val Asp Lys Leu Ala Ala Ala 
            660                 665                 670         


Leu Glu His His His His His His 
        675                 680 


<210>  14
<211>  349
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fimA expressed His-tagged

<400>  14

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Arg 
            20                  25                  30          


Gly Ser Glu Phe Ser Asn Leu Pro Ser Gly Gly Val Glu Gly Thr Glu 
        35                  40                  45              


Gln Asn Pro Ala Lys Ala Thr Ile Thr Lys Asn Phe Glu Phe Pro Glu 
    50                  55                  60                  


Gly Ile Asn Thr Pro Ser Ala Thr Phe Lys Phe Thr Ala Glu Lys Ile 
65                  70                  75                  80  


Thr Asn Asp Ala Pro Asp Ala Thr Ile Gly Asp Ile Asn Tyr Thr Gln 
                85                  90                  95      


Gly Asp Asn Gly Thr Leu Ser Asn Gly Lys Tyr Ser Val Lys Lys Thr 
            100                 105                 110         


Thr Glu Ile Thr Phe Gly Asn Phe Pro His Ala Gly Glu Tyr Asp Tyr 
        115                 120                 125             


Asn Val Lys Glu Thr Asn Glu Gly Val Gly Gly Ile Thr Tyr Asp Thr 
    130                 135                 140                 


Lys Glu Tyr Lys Val His Val Tyr Val Ala Asn Ser Asn Ala Met Asp 
145                 150                 155                 160 


Gly Lys Thr Tyr Val Lys Ala Ile Thr Ser Glu Asn Gly Gly Glu Lys 
                165                 170                 175     


Ala Pro Ile Glu Phe Val Asn Thr Tyr Lys Lys Asp Thr Ser Leu Leu 
            180                 185                 190         


Ile Glu Lys Asn Val Ile Gly Asp Leu Ala Asp Leu Thr Lys Gln Phe 
        195                 200                 205             


Glu Phe Gln Ile Asn Leu Lys Lys Ser Ala Thr Ser Asp Ile Thr Lys 
    210                 215                 220                 


Phe Glu Gly Asn Ile Ile Arg Lys Asp Gly Lys Ile Glu Pro Val Thr 
225                 230                 235                 240 


Tyr Thr Ala Glu Asn Thr Glu Thr Phe Lys Leu Ala Asn Gly Asp Lys 
                245                 250                 255     


Leu Lys Phe Glu Ser Ile Pro Ala Gly Thr Lys Tyr Glu Val Lys Glu 
            260                 265                 270         


Ile Gly Ala Ser Asp Gly Tyr Thr Pro Ser Ile Thr Val Ile Glu Asn 
        275                 280                 285             


Gly Asn Glu Thr Ser Asn Asn Arg Thr Val Ala Glu Lys Asp Gly Ile 
    290                 295                 300                 


Ser Ser Lys Ser Asn Ser Asn Asp Asn Leu Ile Gly Glu Gly Glu Asn 
305                 310                 315                 320 


Lys Val Thr Phe Thr Asn Thr Tyr Asn Asp Lys Pro Ile Thr Val Asp 
                325                 330                 335     


Lys Leu Ala Ala Ala Leu Glu His His His His His His 
            340                 345                 


<210>  15
<211>  207
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fimB expressed His-tagged

<400>  15

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Arg 
            20                  25                  30          


Gly Ser Glu Phe Thr Glu Asn Thr Ala Asn Ile Pro Leu Ile Val Arg 
        35                  40                  45              


Gln Glu Phe Asn Val Tyr Thr Lys Asp Ser Lys Ala Ile Asp Met Ile 
    50                  55                  60                  


Gly Lys Tyr Glu Leu Lys Ala Ile Ser Glu Asn Ala Pro Met Pro Glu 
65                  70                  75                  80  


Glu Ser Lys Asn Gly Ser Phe Ile Phe Asn Ile Asp Gly Asn Asp Lys 
                85                  90                  95      


Gln Phe Thr Ile Pro Leu Ala Tyr Thr His Gly Gly Val Tyr Ile Tyr 
            100                 105                 110         


Gln Ile Gln Gln Ile Thr Gln Ser Lys Asp Asn Tyr Ile Tyr Asp Lys 
        115                 120                 125             


Asn Ser Tyr Lys Ile Thr Val Tyr Val Lys Asn Ala Glu Asn Asn His 
    130                 135                 140                 


Leu Ile Pro Gln Ile Ile Val Lys Asn Glu Asn Asn Glu Lys Cys Glu 
145                 150                 155                 160 


Glu Ile Cys Phe Tyr Asn Ile Tyr Lys Gln Lys Asn Lys Ile Asn Glu 
                165                 170                 175     


Ile Ser Lys Thr Pro Tyr Lys Pro Asn Gly Ile Asn Val Pro Lys Thr 
            180                 185                 190         


Val Asp Lys Leu Ala Ala Ala Leu Glu His His His His His His 
        195                 200                 205         


