                         SEQUENCE LISTING

<110>  THE JAMES HUTTON INSTITUTE
 
<120>  NANONETS AND SPHERICAL PARTICLES

<130>  P184655.WO.01

<150>  GB1708675.2
<151>  2017-05-31

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  139
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyCatcher Sequence

<400>  1

Met Ser Tyr Tyr His His His His His His Asp Tyr Asp Ile Pro Thr 
1               5                   10                  15      


Thr Glu Asn Leu Tyr Phe Gln Gly Ala Met Val Asp Thr Leu Ser Gly 
            20                  25                  30          


Leu Ser Ser Glu Gln Gly Gln Ser Gly Asp Met Thr Ile Glu Glu Asp 
        35                  40                  45              


Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu 
    50                  55                  60                  


Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile 
65                  70                  75                  80  


Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro 
                85                  90                  95      


Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val 
            100                 105                 110         


Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val 
        115                 120                 125             


Asn Gly Lys Ala Thr Lys Gly Asp Ala His Ile 
    130                 135                 


<210>  2
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyTag

<400>  2

Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
1               5                   10              


<210>  3
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyTag truncated

<400>  3

Ala His Ile Val Met Val Asp Ala 
1               5               


<210>  4
<211>  159
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TMV CP (modified for self assembly in bacteria)

<400>  4

Met Ser Tyr Ser Ile Thr Thr Pro Ser Gln Phe Val Phe Leu Ser Ser 
1               5                   10                  15      


Ala Trp Ala Asp Pro Ile Glu Leu Ile Asn Leu Cys Thr Asn Ala Leu 
            20                  25                  30          


Gly Asn Gln Phe Gln Thr Gln Gln Ala Arg Thr Val Val Gln Arg Gln 
        35                  40                  45              


Phe Ser Gln Val Trp Lys Pro Ser Pro Gln Val Thr Val Arg Phe Pro 
    50                  55                  60                  


Asp Ser Asp Phe Lys Val Tyr Arg Tyr Asn Ala Val Leu Asn Pro Leu 
65                  70                  75                  80  


Val Thr Ala Leu Leu Gly Ala Phe Asp Thr Arg Asn Arg Ile Ile Glu 
                85                  90                  95      


Val Glu Asn Gln Ala Asn Pro Thr Thr Ala Glu Thr Leu Asp Ala Thr 
            100                 105                 110         


Arg Arg Val Asp Asp Ala Thr Val Ala Ile Arg Ser Ala Ile Asn Asn 
        115                 120                 125             


Leu Ile Val Glu Leu Ile Arg Gly Thr Gly Ser Tyr Asn Arg Ser Ser 
    130                 135                 140                 


Phe Glu Ser Ser Ser Gly Leu Val Trp Thr Ser Gly Pro Ala Thr 
145                 150                 155                 


<210>  5
<211>  172
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TMV CP with C-terminal SpyTag

<400>  5

Met Ser Tyr Ser Ile Thr Thr Pro Ser Gln Phe Val Phe Leu Ser Ser 
1               5                   10                  15      


Ala Trp Ala Asp Pro Ile Glu Leu Ile Asn Leu Cys Thr Asn Ala Leu 
            20                  25                  30          


Gly Asn Gln Phe Gln Thr Gln Gln Ala Arg Thr Val Val Gln Arg Gln 
        35                  40                  45              


Phe Ser Gln Val Trp Lys Pro Ser Pro Gln Val Thr Val Arg Phe Pro 
    50                  55                  60                  


Asp Ser Asp Phe Lys Val Tyr Arg Tyr Asn Ala Val Leu Asn Pro Leu 
65                  70                  75                  80  


Val Thr Ala Leu Leu Gly Ala Phe Asp Thr Arg Asn Arg Ile Ile Glu 
                85                  90                  95      


Val Glu Asn Gln Ala Asn Pro Thr Thr Ala Glu Thr Leu Asp Ala Thr 
            100                 105                 110         


Arg Arg Val Asp Asp Ala Thr Val Ala Ile Arg Ser Ala Ile Asn Asn 
        115                 120                 125             


Leu Ile Val Glu Leu Ile Arg Gly Thr Gly Ser Tyr Asn Arg Ser Ser 
    130                 135                 140                 


Phe Glu Ser Ser Ser Gly Leu Val Trp Thr Ser Gly Pro Ala Thr Ala 
145                 150                 155                 160 


His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
                165                 170         


<210>  6
<211>  265
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GFP sequence

<400>  6

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser 
225                 230                 235                 240 


Gly Leu Arg Ser Arg Ala Gln Ala Ser Asn Ser Ala Val Asp Gly Thr 
                245                 250                 255     


Ala Gly Pro Gly Ser Thr Gly Ser Arg 
            260                 265 


<210>  7
<211>  407
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyCatcher-GFP

<400>  7

Met Ser Tyr Tyr His His His His His His Asp Tyr Asp Ile Pro Thr 
1               5                   10                  15      


Thr Glu Asn Leu Tyr Phe Gln Gly Ala Met Val Asp Thr Leu Ser Gly 
            20                  25                  30          


Leu Ser Ser Glu Gln Gly Gln Ser Gly Asp Met Thr Ile Glu Glu Asp 
        35                  40                  45              


Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu 
    50                  55                  60                  


Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile 
65                  70                  75                  80  


Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro 
                85                  90                  95      


Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val 
            100                 105                 110         


Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val 
        115                 120                 125             


Asn Gly Lys Ala Thr Lys Gly Asp Ala His Ile Ala Thr Cys Met Val 
    130                 135                 140                 


Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu 
145                 150                 155                 160 


Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly 
                165                 170                 175     


Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr 
            180                 185                 190         


Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr 
        195                 200                 205             


Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His 
    210                 215                 220                 


Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr 
225                 230                 235                 240 


Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys 
                245                 250                 255     


Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp 
            260                 265                 270         


Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr 
        275                 280                 285             


Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile 
    290                 295                 300                 


Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln 
305                 310                 315                 320 


Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val 
                325                 330                 335     


Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys 
            340                 345                 350         


Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr 
        355                 360                 365             


Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser Gly Leu 
    370                 375                 380                 


Arg Ser Arg Ala Gln Ala Ser Asn Ser Ala Val Asp Gly Thr Ala Gly 
385                 390                 395                 400 


Pro Gly Ser Thr Gly Ser Arg 
                405         


<210>  8
<211>  340
<212>  PRT
<213>  Streptococcus pyogenes

<400>  8

Met Lys Leu Arg His Leu Leu Leu Thr Gly Ala Ala Leu Thr Ser Phe 
1               5                   10                  15      


Ala Ala Thr Thr Val His Gly Glu Thr Val Val Asn Gly Ala Lys Leu 
            20                  25                  30          


Thr Val Thr Lys Asn Leu Asp Leu Val Asn Ser Asn Ala Leu Ile Pro 
        35                  40                  45              


Asn Thr Asp Phe Thr Phe Lys Ile Glu Pro Asp Thr Thr Val Asn Glu 
    50                  55                  60                  


Asp Gly Asn Lys Phe Lys Gly Val Ala Leu Asn Thr Pro Met Thr Lys 
65                  70                  75                  80  


Val Thr Tyr Thr Asn Ser Asp Lys Gly Gly Ser Asn Thr Lys Thr Ala 
                85                  90                  95      


Glu Phe Asp Phe Ser Glu Val Thr Phe Glu Lys Pro Gly Val Tyr Tyr 
            100                 105                 110         


Tyr Lys Val Thr Glu Glu Lys Ile Asp Lys Val Pro Gly Val Ser Tyr 
        115                 120                 125             


Asp Thr Thr Ser Tyr Thr Val Gln Val His Val Leu Trp Asn Glu Glu 
    130                 135                 140                 


Gln Gln Lys Pro Val Ala Thr Tyr Ile Val Gly Tyr Lys Glu Gly Ser 
145                 150                 155                 160 


Lys Val Pro Ile Gln Phe Lys Asn Ser Leu Asp Ser Thr Thr Leu Thr 
                165                 170                 175     


Val Lys Lys Lys Val Ser Gly Thr Gly Gly Asp Arg Ser Lys Asp Phe 
            180                 185                 190         


Asn Phe Gly Leu Thr Leu Lys Ala Asn Gln Tyr Tyr Lys Ala Ser Glu 
        195                 200                 205             


Lys Val Met Ile Glu Lys Thr Thr Lys Gly Gly Gln Ala Pro Val Gln 
    210                 215                 220                 


Thr Glu Ala Ser Ile Asp Gln Leu Tyr His Phe Thr Leu Lys Asp Gly 
225                 230                 235                 240 


Glu Ser Ile Lys Val Thr Asn Leu Pro Val Gly Val Asp Tyr Val Val 
                245                 250                 255     


Thr Glu Asp Asp Tyr Lys Ser Glu Lys Tyr Thr Thr Asn Val Glu Val 
            260                 265                 270         


Ser Pro Gln Asp Gly Ala Val Lys Asn Ile Ala Gly Asn Ser Thr Glu 
        275                 280                 285             


Gln Glu Thr Ser Thr Asp Lys Asp Met Thr Ile Thr Phe Thr Asn Lys 
    290                 295                 300                 


Lys Asp Phe Glu Val Pro Thr Gly Val Ala Met Thr Val Ala Pro Tyr 
305                 310                 315                 320 


Ile Ala Leu Gly Ile Val Ala Val Gly Gly Ala Leu Tyr Phe Val Lys 
                325                 330                 335     


Lys Lys Asn Ala 
            340 


<210>  9
<211>  674
<212>  PRT
<213>  Enterococcus faecalis

<400>  9

Met Thr Lys Ser Val Lys Phe Leu Val Leu Leu Leu Val Met Ile Leu 
1               5                   10                  15      


Pro Ile Ala Gly Ala Leu Leu Ile Gly Pro Ile Ser Phe Gly Ala Glu 
            20                  25                  30          


Leu Ser Lys Ser Ser Ile Val Asp Lys Val Glu Leu Asp His Thr Thr 
        35                  40                  45              


Leu Tyr Gln Gly Glu Met Thr Ser Ile Lys Val Ser Phe Ser Asp Lys 
    50                  55                  60                  


Glu Asn Gln Lys Ile Lys Pro Gly Asp Thr Ile Thr Leu Thr Leu Pro 
65                  70                  75                  80  


Asp Ala Leu Val Gly Met Thr Glu Asn Asp Ser Ser Pro Arg Lys Ile 
                85                  90                  95      


Asn Leu Asn Gly Leu Gly Glu Val Phe Ile Tyr Lys Asp His Val Val 
            100                 105                 110         


Ala Thr Phe Asn Glu Lys Val Glu Ser Leu His Asn Val Asn Gly His 
        115                 120                 125             


Phe Ser Phe Gly Ile Lys Thr Leu Ile Thr Asn Ser Ser Gln Pro Asn 
    130                 135                 140                 


Val Ile Glu Thr Asp Phe Gly Thr Ala Thr Ala Thr Gln Arg Leu Thr 
145                 150                 155                 160 


Ile Glu Gly Val Thr Asn Thr Glu Thr Gly Gln Ile Glu Arg Asp Tyr 
                165                 170                 175     


Pro Phe Phe Tyr Lys Val Gly Asp Leu Ala Gly Glu Ser Asn Gln Val 
            180                 185                 190         


Arg Trp Phe Leu Asn Val Asn Leu Asn Lys Ser Asp Val Thr Glu Asp 
        195                 200                 205             


Ile Ser Ile Ala Asp Arg Gln Gly Ser Gly Gln Gln Leu Asn Lys Glu 
    210                 215                 220                 


Ser Phe Thr Phe Asp Ile Val Asn Asp Lys Glu Thr Lys Tyr Ile Ser 
225                 230                 235                 240 


Leu Ala Glu Phe Glu Gln Gln Gly Tyr Gly Lys Ile Asp Phe Val Thr 
                245                 250                 255     


Asp Asn Asp Phe Asn Leu Arg Phe Tyr Arg Asp Lys Ala Arg Phe Thr 
            260                 265                 270         


Ser Phe Ile Val Arg Tyr Thr Ser Thr Ile Thr Glu Ala Gly Gln His 
        275                 280                 285             


Gln Ala Thr Phe Glu Asn Ser Tyr Asp Ile Asn Tyr Gln Leu Asn Asn 
    290                 295                 300                 


Gln Asp Ala Thr Asn Glu Lys Asn Thr Ser Gln Val Lys Asn Val Phe 
305                 310                 315                 320 


Val Glu Gly Glu Ala Ser Gly Asn Gln Asn Val Glu Met Pro Thr Glu 
                325                 330                 335     


Glu Ser Leu Asp Ile Pro Leu Glu Thr Ile Asp Glu Trp Glu Pro Lys 
            340                 345                 350         


Thr Pro Thr Ser Glu Gln Ala Thr Glu Thr Ser Glu Lys Thr Asp Thr 
        355                 360                 365             


Thr Glu Thr Ala Glu Ser Ser Gln Pro Glu Val His Val Ser Pro Thr 
    370                 375                 380                 


Glu Glu Glu Asn Pro Asp Glu Gly Glu Thr Leu Gly Thr Ile Glu Pro 
385                 390                 395                 400 


Ile Ile Pro Glu Lys Pro Ser Val Thr Thr Glu Glu Asn Gly Thr Thr 
                405                 410                 415     


Glu Thr Ala Glu Ser Ser Gln Pro Glu Val His Val Ser Pro Thr Glu 
            420                 425                 430         


Glu Glu Asn Pro Asp Glu Ser Glu Thr Leu Gly Thr Ile Glu Pro Ile 
        435                 440                 445             


Ile Pro Glu Lys Pro Ser Val Thr Thr Glu Glu Asn Gly Thr Thr Glu 
    450                 455                 460                 


Thr Ala Glu Ser Ser Gln Pro Glu Val His Val Ser Pro Ala Glu Glu 
465                 470                 475                 480 


Glu Asn Pro Asp Glu Ser Glu Thr Leu Gly Thr Ile Leu Pro Ile Leu 
                485                 490                 495     


Pro Glu Lys Pro Ser Val Thr Thr Glu Glu Asn Gly Thr Thr Glu Thr 
            500                 505                 510         


Ala Glu Ser Ser Gln Pro Glu Val His Val Ser Pro Thr Glu Glu Glu 
        515                 520                 525             


Asn Pro Asp Glu Ser Glu Thr Leu Gly Thr Ile Ala Pro Ile Ile Pro 
    530                 535                 540                 


Glu Lys Pro Ser Val Thr Thr Glu Glu Asn Gly Ile Thr Glu Thr Ala 
545                 550                 555                 560 


Glu Ser Ser Gln Pro Glu Val His Val Ser Pro Thr Lys Glu Ile Thr 
                565                 570                 575     


Thr Thr Glu Lys Lys Gln Pro Ser Thr Glu Thr Thr Val Glu Lys Asn 
            580                 585                 590         


Lys Asn Val Thr Ser Lys Asn Gln Pro Gln Ile Leu Asn Ala Pro Leu 
        595                 600                 605             


Asn Thr Leu Lys Asn Glu Gly Ser Pro Gln Leu Ala Pro Gln Leu Leu 
    610                 615                 620                 


Ser Glu Pro Ile Gln Lys Leu Asn Glu Ala Asn Gly Gln Arg Glu Leu 
625                 630                 635                 640 


Pro Lys Thr Gly Thr Thr Lys Thr Pro Phe Met Leu Ile Ala Gly Ile 
                645                 650                 655     


Leu Ala Ser Thr Phe Ala Val Leu Gly Val Ser Tyr Leu Gln Ile Arg 
            660                 665                 670         


Lys Asn 
        


<210>  10
<211>  313
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TMV CP-GGGGS-SpyCatcher His

<400>  10

Met Ser Tyr Ser Ile Thr Thr Pro Ser Gln Phe Val Phe Leu Ser Ser 
1               5                   10                  15      


Ala Trp Ala Asp Pro Ile Glu Leu Ile Asn Leu Cys Thr Asn Ala Leu 
            20                  25                  30          


Gly Asn Gln Phe Gln Thr Gln Gln Ala Arg Thr Val Val Gln Arg Gln 
        35                  40                  45              


Phe Ser Gln Val Trp Lys Pro Ser Pro Gln Val Thr Val Arg Phe Pro 
    50                  55                  60                  


Asp Ser Asp Phe Lys Val Tyr Arg Tyr Asn Ala Val Leu Asn Pro Leu 
65                  70                  75                  80  


Val Thr Ala Leu Leu Gly Ala Phe Asp Thr Arg Asn Arg Ile Ile Glu 
                85                  90                  95      


Val Glu Asn Gln Ala Asn Pro Thr Thr Ala Glu Thr Leu Asp Ala Thr 
            100                 105                 110         


Arg Arg Val Asp Asp Ala Thr Val Ala Ile Arg Ser Ala Ile Asn Asn 
        115                 120                 125             


Leu Ile Val Glu Leu Ile Arg Gly Thr Gly Ser Tyr Asn Arg Ser Ser 
    130                 135                 140                 


Phe Glu Ser Ser Ser Gly Leu Val Trp Thr Ser Gly Pro Ala Thr Gly 
145                 150                 155                 160 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met Ser 
                165                 170                 175     


Tyr Tyr His His His His His His Asp Tyr Asp Ile Pro Thr Thr Glu 
            180                 185                 190         


Asn Leu Tyr Phe Gln Gly Ala Met Val Asp Thr Leu Ser Gly Leu Ser 
        195                 200                 205             


Ser Glu Gln Gly Gln Ser Gly Asp Met Thr Ile Glu Glu Asp Ser Ala 
    210                 215                 220                 


Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu Leu Ala 
225                 230                 235                 240 


Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser Thr 
                245                 250                 255     


Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro Gly Lys 
            260                 265                 270         


Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala Thr 
        275                 280                 285             


Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val Asn Gly 
    290                 295                 300                 


Lys Ala Thr Lys Gly Asp Ala His Ile 
305                 310             


<210>  11
<211>  193
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TMV CP-GGGGS-SpyTag His

<400>  11

Met Ser Tyr Ser Ile Thr Thr Pro Ser Gln Phe Val Phe Leu Ser Ser 
1               5                   10                  15      


Ala Trp Ala Asp Pro Ile Glu Leu Ile Asn Leu Cys Thr Asn Ala Leu 
            20                  25                  30          


Gly Asn Gln Phe Gln Thr Gln Gln Ala Arg Thr Val Val Gln Arg Gln 
        35                  40                  45              


Phe Ser Gln Val Trp Lys Pro Ser Pro Gln Val Thr Val Arg Phe Pro 
    50                  55                  60                  


Asp Ser Asp Phe Lys Val Tyr Arg Tyr Asn Ala Val Leu Asn Pro Leu 
65                  70                  75                  80  


Val Thr Ala Leu Leu Gly Ala Phe Asp Thr Arg Asn Arg Ile Ile Glu 
                85                  90                  95      


Val Glu Asn Gln Ala Asn Pro Thr Thr Ala Glu Thr Leu Asp Ala Thr 
            100                 105                 110         


Arg Arg Val Asp Asp Ala Thr Val Ala Ile Arg Ser Ala Ile Asn Asn 
        115                 120                 125             


Leu Ile Val Glu Leu Ile Arg Gly Thr Gly Ser Tyr Asn Arg Ser Ser 
    130                 135                 140                 


Phe Glu Ser Ser Ser Gly Leu Val Trp Thr Ser Gly Pro Ala Thr Gly 
145                 150                 155                 160 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala His 
                165                 170                 175     


Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys His His His His His 
            180                 185                 190         


His 
    


<210>  12
<211>  169
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  11-20 SpyCatcher

<400>  12

Met Ser Tyr Tyr His His His His His His Asp Tyr Asp Ile Pro Thr 
1               5                   10                  15      


Thr Glu Asn Leu Tyr Phe Gln Gly Ala Met Val Asp Thr Leu Ser Gly 
            20                  25                  30          


Leu Ser Ser Glu Gln Gly Gln Ser Gly Asp Met Thr Ile Glu Glu Asp 
        35                  40                  45              


Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu 
    50                  55                  60                  


Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile 
65                  70                  75                  80  


Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro 
                85                  90                  95      


Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val 
            100                 105                 110         


Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val 
        115                 120                 125             


Asn Gly Lys Ala Thr Lys Gly Asp Ala His Ile Leu Asp Ile Val Ala 
    130                 135                 140                 


Asp Ile Asn Gly Val Gln Ile Glu Val Pro Ile Leu Pro Thr Thr Lys 
145                 150                 155                 160 


Ala Lys Ile Ile Ala Lys Ile Ile Gly 
                165                 


<210>  13
<211>  358
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GalEst

<400>  13

Met His His His His His His Ala His Ile Val Met Val Asp Ala Tyr 
1               5                   10                  15      


Lys Pro Thr Lys Met Arg Val Leu Val Thr Gly Gly Ser Gly Tyr Ile 
            20                  25                  30          


Gly Ser His Thr Cys Val Gln Leu Leu Gln Asn Gly His Asp Val Ile 
        35                  40                  45              


Ile Leu Asp Asn Leu Cys Asn Ser Lys Arg Ser Val Leu Pro Val Ile 
    50                  55                  60                  


Glu Arg Leu Gly Gly Lys His Pro Thr Phe Val Glu Gly Asp Ile Arg 
65                  70                  75                  80  


Asn Glu Ala Leu Met Thr Glu Ile Leu His Asp His Ala Ile Asp Thr 
                85                  90                  95      


Val Ile His Phe Ala Gly Leu Lys Ala Val Gly Glu Ser Val Gln Lys 
            100                 105                 110         


Pro Leu Glu Tyr Tyr Asp Asn Asn Val Asn Gly Thr Leu Arg Leu Ile 
        115                 120                 125             


Ser Ala Met Arg Ala Ala Asn Val Lys Asn Phe Ile Phe Ser Ser Ser 
    130                 135                 140                 


Ala Thr Val Tyr Gly Asp Gln Pro Lys Ile Pro Tyr Val Glu Ser Phe 
145                 150                 155                 160 


Pro Thr Gly Thr Pro Gln Ser Pro Tyr Gly Lys Ser Lys Leu Met Val 
                165                 170                 175     


Glu Gln Ile Leu Thr Asp Leu Gln Lys Ala Gln Pro Asp Trp Ser Ile 
            180                 185                 190         


Ala Leu Leu Arg Tyr Phe Asn Pro Val Gly Ala His Pro Ser Gly Asp 
        195                 200                 205             


Met Gly Glu Asp Pro Gln Gly Ile Pro Asn Asn Leu Met Pro Tyr Ile 
    210                 215                 220                 


Ala Gln Val Ala Val Gly Arg Arg Asp Ser Leu Ala Ile Phe Gly Asn 
225                 230                 235                 240 


Asp Tyr Pro Thr Glu Asp Gly Thr Gly Val Arg Asp Tyr Ile His Val 
                245                 250                 255     


Met Asp Leu Ala Asp Gly His Val Val Ala Met Glu Lys Leu Ala Asn 
            260                 265                 270         


Lys Pro Gly Val His Ile Tyr Asn Leu Gly Ala Gly Val Gly Asn Ser 
        275                 280                 285             


Val Leu Asp Val Val Asn Ala Phe Ser Lys Ala Cys Gly Lys Pro Val 
    290                 295                 300                 


Asn Tyr His Phe Ala Pro Arg Arg Glu Gly Asp Leu Pro Ala Tyr Trp 
305                 310                 315                 320 


Ala Asp Ala Ser Lys Ala Asp Arg Glu Leu Asn Trp Arg Val Thr Arg 
                325                 330                 335     


Thr Leu Asp Glu Met Ala Gln Asp Thr Trp His Trp Gln Ser Arg His 
            340                 345                 350         


Pro Gln Gly Tyr Pro Asp 
        355             


