                         SEQUENCE LISTING

<110>  University of Cape Town
 
<120>  EXPRESSION OF CHAPERONE PROTEINS IN PLANTA FOR INCREASED 
       EXPRESSION OF HETEROLOGOUS POLYPEPTIDES OF INTEREST

<130>  P34291GB1/SNB

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  1266
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence of humanised calreticulin

<400>  1
accggtatgc tgctgagcgt gcctctgctg ctggggctgc tggggctggc cgtcgccgaa       60

cctgctgtct acttcaagga acagtttctg gatggcgacg gatggaccag ccggtggatc      120

gagagcaagc acaagtccga tttcggcaag tttgtgctga gctccggcaa gttctatggc      180

gatgaggaga aggacaaggg cctgcagaca agccaggacg cccggttcta cgccctgtct      240

gccagcttcg agccattttc caacaagggc cagaccctgg tggtgcagtt cacagtgaag      300

cacgagcaga acatcgattg cggcggcggc tatgtgaagc tgtttcccaa ttccctggat      360

cagaccgaca tgcacggcga ctctgagtac aacatcatgt tcggcccaga tatctgcggc      420

cccggcacaa agaaggtgca cgtgatcttt aattataagg gcaagaacgt gctgatcaat      480

aaggacatca ggtgtaagga cgatgagttc acccacctgt acacactgat cgtgcgccct      540

gacaacacct atgaggtgaa gatcgataat tcccaggtgg agtccggctc tctggaggac      600

gattgggatt ttctgccccc taagaagatc aaggaccctg atgcctctaa gccagaggac      660

tgggatgaga gggccaagat cgacgatccc acagacagca agcctgagga ctgggataag      720

cccgagcaca tccctgaccc agatgccaag aagcccgaag actgggatga ggagatggat      780

ggcgagtggg agccacccgt gatccagaac cccgagtaca agggcgagtg gaagcctaga      840

cagatcgata atccagacta taagggcacc tggatccacc cagagatcga taaccccgag      900

tactctcccg accctagcat ctacgcctat gataatttcg gcgtgctggg cctggacctg      960

tggcaggtga agtctggcac catcttcgac aactttctga tcacaaatga tgaggcctac     1020

gccgaggagt ttggcaatga gacatggggc gtgacaaagg ccgccgagaa gcagatgaag     1080

gataagcagg acgaggagca gcggctgaag gaagaggagg aggacaagaa gagaaaggag     1140

gaggaggagg ccgaggataa ggaggacgat gaggacaagg atgaggacga ggaggacgag     1200

gaggataagg aggaagatga agaggaggat gtcccagggc aggcaaaaga tgaactgtga     1260

ctcgag                                                                1266


<210>  2
<211>  1791
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence of humanised calnexin

<400>  2
accggtatgg aggggaaatg gctgctgtgc atgctgctgg tgctgggaac tgctattgtg       60

gaggctcacg acggacacga tgacgatgtg attgacatcg aggacgatct ggacgatgtg      120

atcgaggagg tggaggacag caagcctgat accacagccc cccctagctc cccaaaggtg      180

acctacaagg cccccgtgcc tacaggcgag gtgtatttcg ccgactcctt tgataggggc      240

accctgagcg gatggatcct gtccaaggcc aagaaggacg atacagacga tgagatcgcc      300

aagtacgacg gcaagtggga agtggaggag atgaaggagt ctaagctgcc tggcgataag      360

ggcctggtgc tgatgtcccg ggccaagcac cacgccatct ctgccaagct gaataagcca      420

ttcctgtttg acaccaagcc cctgatcgtg cagtacgagg tgaacttcca gaatggcatc      480

gagtgcggcg gcgcctatgt gaagctgctg tccaagacac cagagctgaa tctggaccag      540

ttccacgata agacccctta cacaatcatg tttggcccag acaagtgtgg cgaggattat      600

aagctgcact tcatctttag acacaagaac ccaaagaccg gcatctatga ggagaagcac      660

gccaagaggc ccgacgccga tctgaagacc tacttcacag acaagaagac ccacctgtat      720

acactgatcc tgaacccaga caattctttt gagatcctgg tggatcagtc cgtggtgaac      780

tctggcaatc tgctgaacga tatgacccca cccgtgaatc ccagcaggga gatcgaggac      840

cccgaggatc gcaagcctga ggactgggat gagcggccca agatcccaga cccagaggca      900

gtgaagcctg acgattggga cgaggatgcc cctgccaaga tcccagatga ggaggccaca      960

aagcccgagg gctggctgga cgatgagcct gagtacgtgc ctgacccaga tgccgagaag     1020

cccgaggact gggatgagga catggatggc gagtgggagg ccccacagat cgcaaaccca     1080

agatgcgaga gcgcccctgg atgtggcgtg tggcagaggc ctgtgatcga caacccaaat     1140

tacaagggca agtggaagcc tccaatgatc gataatccat cctatcaggg catctggaag     1200

ccccgcaaga tccccaaccc tgacttcttt gaggatctgg agcccttccg gatgacccct     1260

ttttctgcca tcggcctgga gctgtggtct atgacaagcg acatcttctt tgataacttc     1320

atcatctgcg ccgaccggag aatcgtggac gattgggcca acgacggatg gggcctgaag     1380

aaggcagcag atggagcagc agagccagga gtggtgggac agatgatcga ggcagcagag     1440

gagcggccct ggctgtgggt ggtgtacatc ctgaccgtgg ccctgcccgt gttcctggtc     1500

atcctgttct gctgttctgg caagaagcag accagcggca tggagtataa gaagacagac     1560

gccccacagc ccgatgtgaa agaggaggag gaggagaagg aggaggagaa ggacaagggc     1620

gatgaggagg aggagggcga ggagaagctg gaggagaagc agaagagcga cgccgaggag     1680

gatggcggca cagtgtccca ggaggaggag gaccggaagc ctaaggcaga agaagacgaa     1740

atcctgaatc ggtcaccaag aaatagaaaa ccacggaggg aatgactcga g              1791


<210>  3
<211>  417
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Leu Leu Ser Val Pro Leu Leu Leu Gly Leu Leu Gly Leu Ala Val 
1               5                   10                  15      


Ala Glu Pro Ala Val Tyr Phe Lys Glu Gln Phe Leu Asp Gly Asp Gly 
            20                  25                  30          


Trp Thr Ser Arg Trp Ile Glu Ser Lys His Lys Ser Asp Phe Gly Lys 
        35                  40                  45              


Phe Val Leu Ser Ser Gly Lys Phe Tyr Gly Asp Glu Glu Lys Asp Lys 
    50                  55                  60                  


Gly Leu Gln Thr Ser Gln Asp Ala Arg Phe Tyr Ala Leu Ser Ala Ser 
65                  70                  75                  80  


Phe Glu Pro Phe Ser Asn Lys Gly Gln Thr Leu Val Val Gln Phe Thr 
                85                  90                  95      


Val Lys His Glu Gln Asn Ile Asp Cys Gly Gly Gly Tyr Val Lys Leu 
            100                 105                 110         


Phe Pro Asn Ser Leu Asp Gln Thr Asp Met His Gly Asp Ser Glu Tyr 
        115                 120                 125             


Asn Ile Met Phe Gly Pro Asp Ile Cys Gly Pro Gly Thr Lys Lys Val 
    130                 135                 140                 


His Val Ile Phe Asn Tyr Lys Gly Lys Asn Val Leu Ile Asn Lys Asp 
145                 150                 155                 160 


Ile Arg Cys Lys Asp Asp Glu Phe Thr His Leu Tyr Thr Leu Ile Val 
                165                 170                 175     


Arg Pro Asp Asn Thr Tyr Glu Val Lys Ile Asp Asn Ser Gln Val Glu 
            180                 185                 190         


Ser Gly Ser Leu Glu Asp Asp Trp Asp Phe Leu Pro Pro Lys Lys Ile 
        195                 200                 205             


Lys Asp Pro Asp Ala Ser Lys Pro Glu Asp Trp Asp Glu Arg Ala Lys 
    210                 215                 220                 


Ile Asp Asp Pro Thr Asp Ser Lys Pro Glu Asp Trp Asp Lys Pro Glu 
225                 230                 235                 240 


His Ile Pro Asp Pro Asp Ala Lys Lys Pro Glu Asp Trp Asp Glu Glu 
                245                 250                 255     


Met Asp Gly Glu Trp Glu Pro Pro Val Ile Gln Asn Pro Glu Tyr Lys 
            260                 265                 270         


Gly Glu Trp Lys Pro Arg Gln Ile Asp Asn Pro Asp Tyr Lys Gly Thr 
        275                 280                 285             


Trp Ile His Pro Glu Ile Asp Asn Pro Glu Tyr Ser Pro Asp Pro Ser 
    290                 295                 300                 


Ile Tyr Ala Tyr Asp Asn Phe Gly Val Leu Gly Leu Asp Leu Trp Gln 
305                 310                 315                 320 


Val Lys Ser Gly Thr Ile Phe Asp Asn Phe Leu Ile Thr Asn Asp Glu 
                325                 330                 335     


Ala Tyr Ala Glu Glu Phe Gly Asn Glu Thr Trp Gly Val Thr Lys Ala 
            340                 345                 350         


Ala Glu Lys Gln Met Lys Asp Lys Gln Asp Glu Glu Gln Arg Leu Lys 
        355                 360                 365             


Glu Glu Glu Glu Asp Lys Lys Arg Lys Glu Glu Glu Glu Ala Glu Asp 
    370                 375                 380                 


Lys Glu Asp Asp Glu Asp Lys Asp Glu Asp Glu Glu Asp Glu Glu Asp 
385                 390                 395                 400 


Lys Glu Glu Asp Glu Glu Glu Asp Val Pro Gly Gln Ala Lys Asp Glu 
                405                 410                 415     


Leu 
    


<210>  4
<211>  592
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Glu Gly Lys Trp Leu Leu Cys Met Leu Leu Val Leu Gly Thr Ala 
1               5                   10                  15      


Ile Val Glu Ala His Asp Gly His Asp Asp Asp Val Ile Asp Ile Glu 
            20                  25                  30          


Asp Asp Leu Asp Asp Val Ile Glu Glu Val Glu Asp Ser Lys Pro Asp 
        35                  40                  45              


Thr Thr Ala Pro Pro Ser Ser Pro Lys Val Thr Tyr Lys Ala Pro Val 
    50                  55                  60                  


Pro Thr Gly Glu Val Tyr Phe Ala Asp Ser Phe Asp Arg Gly Thr Leu 
65                  70                  75                  80  


Ser Gly Trp Ile Leu Ser Lys Ala Lys Lys Asp Asp Thr Asp Asp Glu 
                85                  90                  95      


Ile Ala Lys Tyr Asp Gly Lys Trp Glu Val Glu Glu Met Lys Glu Ser 
            100                 105                 110         


Lys Leu Pro Gly Asp Lys Gly Leu Val Leu Met Ser Arg Ala Lys His 
        115                 120                 125             


His Ala Ile Ser Ala Lys Leu Asn Lys Pro Phe Leu Phe Asp Thr Lys 
    130                 135                 140                 


Pro Leu Ile Val Gln Tyr Glu Val Asn Phe Gln Asn Gly Ile Glu Cys 
145                 150                 155                 160 


Gly Gly Ala Tyr Val Lys Leu Leu Ser Lys Thr Pro Glu Leu Asn Leu 
                165                 170                 175     


Asp Gln Phe His Asp Lys Thr Pro Tyr Thr Ile Met Phe Gly Pro Asp 
            180                 185                 190         


Lys Cys Gly Glu Asp Tyr Lys Leu His Phe Ile Phe Arg His Lys Asn 
        195                 200                 205             


Pro Lys Thr Gly Ile Tyr Glu Glu Lys His Ala Lys Arg Pro Asp Ala 
    210                 215                 220                 


Asp Leu Lys Thr Tyr Phe Thr Asp Lys Lys Thr His Leu Tyr Thr Leu 
225                 230                 235                 240 


Ile Leu Asn Pro Asp Asn Ser Phe Glu Ile Leu Val Asp Gln Ser Val 
                245                 250                 255     


Val Asn Ser Gly Asn Leu Leu Asn Asp Met Thr Pro Pro Val Asn Pro 
            260                 265                 270         


Ser Arg Glu Ile Glu Asp Pro Glu Asp Arg Lys Pro Glu Asp Trp Asp 
        275                 280                 285             


Glu Arg Pro Lys Ile Pro Asp Pro Glu Ala Val Lys Pro Asp Asp Trp 
    290                 295                 300                 


Asp Glu Asp Ala Pro Ala Lys Ile Pro Asp Glu Glu Ala Thr Lys Pro 
305                 310                 315                 320 


Glu Gly Trp Leu Asp Asp Glu Pro Glu Tyr Val Pro Asp Pro Asp Ala 
                325                 330                 335     


Glu Lys Pro Glu Asp Trp Asp Glu Asp Met Asp Gly Glu Trp Glu Ala 
            340                 345                 350         


Pro Gln Ile Ala Asn Pro Arg Cys Glu Ser Ala Pro Gly Cys Gly Val 
        355                 360                 365             


Trp Gln Arg Pro Val Ile Asp Asn Pro Asn Tyr Lys Gly Lys Trp Lys 
    370                 375                 380                 


Pro Pro Met Ile Asp Asn Pro Ser Tyr Gln Gly Ile Trp Lys Pro Arg 
385                 390                 395                 400 


Lys Ile Pro Asn Pro Asp Phe Phe Glu Asp Leu Glu Pro Phe Arg Met 
                405                 410                 415     


Thr Pro Phe Ser Ala Ile Gly Leu Glu Leu Trp Ser Met Thr Ser Asp 
            420                 425                 430         


Ile Phe Phe Asp Asn Phe Ile Ile Cys Ala Asp Arg Arg Ile Val Asp 
        435                 440                 445             


Asp Trp Ala Asn Asp Gly Trp Gly Leu Lys Lys Ala Ala Asp Gly Ala 
    450                 455                 460                 


Ala Glu Pro Gly Val Val Gly Gln Met Ile Glu Ala Ala Glu Glu Arg 
465                 470                 475                 480 


Pro Trp Leu Trp Val Val Tyr Ile Leu Thr Val Ala Leu Pro Val Phe 
                485                 490                 495     


Leu Val Ile Leu Phe Cys Cys Ser Gly Lys Lys Gln Thr Ser Gly Met 
            500                 505                 510         


Glu Tyr Lys Lys Thr Asp Ala Pro Gln Pro Asp Val Lys Glu Glu Glu 
        515                 520                 525             


Glu Glu Lys Glu Glu Glu Lys Asp Lys Gly Asp Glu Glu Glu Glu Gly 
    530                 535                 540                 


Glu Glu Lys Leu Glu Glu Lys Gln Lys Ser Asp Ala Glu Glu Asp Gly 
545                 550                 555                 560 


Gly Thr Val Ser Gln Glu Glu Glu Asp Arg Lys Pro Lys Ala Glu Glu 
                565                 570                 575     


Asp Glu Ile Leu Asn Arg Ser Pro Arg Asn Arg Lys Pro Arg Arg Glu 
            580                 585                 590         


<210>  5
<211>  2183
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence of CAP256 SU gp120-HA2 inlcuding terminal 
       restriction sites

<400>  5
tcatgaaagc ttaccggtgc caccatggag tggtcttgga tcttcctgtt tctgctgagc       60

gggactgctg gagtgcattc ttccggaggg ctgtgggtca ctgtctacta tggcgtgcct      120

gtctggagag aggccaagac cacactgttc tgcgcttccg atgcaaagtc ttacgaaaaa      180

gaggtgcaca acgtctgggc cacacatgct tgcgtgccaa ctgaccccaa ccctcaggaa      240

ctggtgctga agaatgtcac cgagaacttt aatatgtgga aaaatgacat ggtggatcag      300

atgcacgagg atatcattag tctgtgggac cagtcactga agccctgcgt gaaactgaca      360

cctctgtgcg tcactctgaa ctgtagcgat gcaaaggtga acattaatgc cacatacaat      420

ggcactcgcg aggaaatcaa aaactgttcc ttcaatgcaa ctaccgaact gagggacaag      480

aagaagaagg agtacgccct gttttatcgc ctggacatcg tgcccctgaa caaggaaggg      540

aacaataaca gtgagtatcg gctgattaac tgcaatacca gcgtgattac ccaggcctgt      600

cctaaagtca ccttcgatcc aattcccatc cactactgcg caccagccgg atatgctatt      660

ctgaagtgta acaacaaaac ttttaacggg accggaccct gcaataacgt gtctacagtc      720

cagtgtactc atggcatcaa gcctgtggtc tcaacccagc tgctgctgaa tgggagcctg      780

gccgaggaag agatcattat cagaagcgag aacctgaccg acaatgtgaa gacaattatc      840

gtccacctga acgaatccgt ggagattaat tgcaccaggc caaacaacaa cacacgaaaa      900

tctattcgga tcggaccagg acagaccttc tacgcaacag gggacattat cggagatatc      960

aggcaggctc attgtaacat ttctgaaatc aagtgggaga aaaccctgca gcgcgtgagt     1020

gaaaagctgc gagagcactt caacaaaaca atcatcttta atcagagctc cggcggggac     1080

ctggaaatca caactcattc attcaactgc ggaggcgagt tcttttactg taacactagc     1140

gatctgttct ttaataagac ctttgacgag acctattcca caggctcaaa cagcactaat     1200

tctaccatta cactgccatg ccgaatcaaa cagattatca acatgtggca ggaagtgggc     1260

cgggcaatgt atgccagccc cattgccgga gagatcacct gtaagtccaa tatcactgga     1320

ctgctgctga ccagagatgg gggaggcaac aattctactg aagagacctt taggcccggg     1380

ggaggcaaca tgagagacaa ttggaggagc gaactgtaca agtataaagt ggtcgaggtg     1440

aagcctctgg gaatcgcacc aaccgaggcc cggagaaggg tggtccagca gggcggtgga     1500

ggctcaggtg gaggcggatc cgagggggga tggcagggaa tggtggacgg gtggtacgga     1560

tatcaccatt caaacgaaca ggggagcgga tatgccgctg acaaggagtc tacacagaaa     1620

gcaatcgatg gggtgactaa caaggtcaat agtatcatcg ataagatgaa tacccagttc     1680

gaagccgtgg gacgcgagtt taacaatctg gaacgccgaa tcgagaacct gaataagaaa     1740

atggaagacg gcttcctgga tgtgtggacc tacaacgctg agctgctggt cctgatggaa     1800

aatgagcgga cactggactt tcacgatagc aacgtgaaga atctgtatga caaagtcaga     1860

ctgcagctga gggataacgc caaggaactg ggcaatgggt gcttcgagtt ttaccataaa     1920

tgcgacaacg aatgtatgga gagtgtgcgc aatggcacct acgattatcc tcagtattca     1980

gaagaggccc ggctgaagag agaagagatt agcggcgtga aactggagtc tattggcatc     2040

taccagatcc tgagtatcta ttcaaccgtg gcttcaagcc tggctctggc aattatggtc     2100

gcaggcctgt ctctgtggat gtgcagcaac gggtccctgc agtgtcgagc ggccgcatga     2160

tttttctgaa ttctagactc gag                                             2183


<210>  6
<211>  739
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of CAP256 SU gp120-HA2 inlcuding terminal 
       restriction sites

<400>  6

Met Glu Thr Glu Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Thr 
1               5                   10                  15      


Ala Gly Val His Ser Ser Gly Gly Leu Trp Val Thr Val Tyr Tyr Gly 
            20                  25                  30          


Val Pro Val Trp Arg Glu Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp 
        35                  40                  45              


Ala Lys Ser Tyr Glu Lys Glu Val His Asn Val Trp Ala Thr His Ala 
    50                  55                  60                  


Cys Val Pro Thr Asp Pro Asn Pro Gln Glu Leu Val Leu Lys Asn Val 
65                  70                  75                  80  


Thr Glu Asn Phe Asn Met Glu Thr Trp Lys Asn Asp Met Glu Thr Val 
                85                  90                  95      


Asp Gln Met Glu Thr His Glu Asp Ile Ile Ser Leu Trp Asp Gln Ser 
            100                 105                 110         


Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu Asn Cys 
        115                 120                 125             


Ser Asp Ala Lys Val Asn Ile Asn Ala Thr Tyr Asn Gly Thr Arg Glu 
    130                 135                 140                 


Glu Ile Lys Asn Cys Ser Phe Asn Ala Thr Thr Glu Leu Arg Asp Lys 
145                 150                 155                 160 


Lys Lys Lys Glu Tyr Ala Leu Phe Tyr Arg Leu Asp Ile Val Pro Leu 
                165                 170                 175     


Asn Lys Glu Gly Asn Asn Asn Ser Glu Tyr Arg Leu Ile Asn Cys Asn 
            180                 185                 190         


Thr Ser Val Ile Thr Gln Ala Cys Pro Lys Val Thr Phe Asp Pro Ile 
        195                 200                 205             


Pro Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn 
    210                 215                 220                 


Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr Val 
225                 230                 235                 240 


Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu 
                245                 250                 255     


Asn Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn Leu 
            260                 265                 270         


Thr Asp Asn Val Lys Thr Ile Ile Val His Leu Asn Glu Ser Val Glu 
        275                 280                 285             


Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg Ile 
    290                 295                 300                 


Gly Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile 
305                 310                 315                 320 


Arg Gln Ala His Cys Asn Ile Ser Glu Ile Lys Trp Glu Lys Thr Leu 
                325                 330                 335     


Gln Arg Val Ser Glu Lys Leu Arg Glu His Phe Asn Lys Thr Ile Ile 
            340                 345                 350         


Phe Asn Gln Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe 
        355                 360                 365             


Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Thr Ser Asp Leu Phe Phe 
    370                 375                 380                 


Asn Lys Thr Phe Asp Glu Thr Tyr Ser Thr Gly Ser Asn Ser Thr Asn 
385                 390                 395                 400 


Ser Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Glu 
                405                 410                 415     


Thr Trp Gln Glu Val Gly Arg Ala Met Glu Thr Tyr Ala Ser Pro Ile 
            420                 425                 430         


Ala Gly Glu Ile Thr Cys Lys Ser Asn Ile Thr Gly Leu Leu Leu Thr 
        435                 440                 445             


Arg Asp Gly Gly Gly Asn Asn Ser Thr Glu Glu Thr Phe Arg Pro Gly 
    450                 455                 460                 


Gly Gly Asn Met Glu Thr Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys 
465                 470                 475                 480 


Tyr Lys Val Val Glu Val Lys Pro Leu Gly Ile Ala Pro Thr Glu Ala 
                485                 490                 495     


Arg Arg Arg Val Val Gln Gln Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            500                 505                 510         


Ser Glu Gly Gly Trp Gln Gly Met Glu Thr Val Asp Gly Trp Tyr Gly 
        515                 520                 525             


Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu 
    530                 535                 540                 


Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile 
545                 550                 555                 560 


Ile Asp Lys Met Glu Thr Asn Thr Gln Phe Glu Ala Val Gly Arg Glu 
                565                 570                 575     


Phe Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu 
            580                 585                 590         


Thr Glu Asp Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu 
        595                 600                 605             


Val Leu Met Glu Thr Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser 
    610                 615                 620                 


Asn Val Lys Asn Leu Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn 
625                 630                 635                 640 


Ala Lys Glu Leu Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                645                 650                 655     


Asn Glu Cys Met Glu Thr Glu Ser Val Arg Asn Gly Thr Tyr Asp Tyr 
            660                 665                 670         


Pro Gln Tyr Ser Glu Glu Ala Arg Leu Lys Arg Glu Glu Ile Ser Gly 
        675                 680                 685             


Val Lys Leu Glu Ser Ile Gly Ile Tyr Gln Ile Leu Ser Ile Tyr Ser 
    690                 695                 700                 


Thr Val Ala Ser Ser Leu Ala Leu Ala Ile Met Glu Thr Val Ala Gly 
705                 710                 715                 720 


Leu Ser Leu Trp Met Glu Thr Cys Ser Asn Gly Ser Leu Gln Cys Arg 
                725                 730                 735     


Ala Ala Ala 
            


<210>  7
<211>  512
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence for gp41tr fragment

<400>  7
tccggagtcg gatccgctgt ggtcggactg ggagcagtgt tcctggggtt tctgggaact       60

gctggcagca ccatgggagc cgcttccatt actctgaccg tgcaggcacg ccagctgctg      120

tctggcatcg tccagcagca gagtaacctg ctgcgggctc ctgaagcaca gcagcatatg      180

ctgcagctga ccgtgtgggg gattaagcag ctgcaggccc gggtcctggc tatcgagaga      240

tacctgaagg atcagcagct gctggggatg tggggatgca gtggcaaact gatttgcacc      300

acaaacgtgt actggaacag cagctggtcc aacaagacat ataatgaaat ctgggacaac      360

atgacttgga tgcagtggga ccgcgagatc gataactaca cagacactat ctataaactg      420

ctggaagtct cacagaaaca gcaggagtca aatgaaaagg acctgctggc actggatgcg      480

gccgcatgat ttttctgaat tctagactcg ag                                    512


<210>  8
<211>  167
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of gp41tr fragment

<400>  8

Ala Val Val Gly Leu Gly Ala Val Phe Leu Gly Phe Leu Gly Thr Ala 
1               5                   10                  15      


Gly Ser Thr Met Glu Thr Gly Ala Ala Ser Ile Thr Leu Thr Val Gln 
            20                  25                  30          


Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu 
        35                  40                  45              


Arg Ala Pro Glu Ala Gln Gln His Met Glu Thr Leu Gln Leu Thr Val 
    50                  55                  60                  


Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Ile Glu Arg Tyr 
65                  70                  75                  80  


Leu Lys Asp Gln Gln Leu Leu Gly Met Glu Thr Trp Gly Cys Ser Gly 
                85                  90                  95      


Lys Leu Ile Cys Thr Thr Asn Val Tyr Trp Asn Ser Ser Trp Ser Asn 
            100                 105                 110         


Lys Thr Tyr Asn Glu Ile Trp Asp Asn Met Glu Thr Thr Trp Met Glu 
        115                 120                 125             


Thr Gln Trp Asp Arg Glu Ile Asp Asn Tyr Thr Asp Thr Ile Tyr Lys 
    130                 135                 140                 


Leu Leu Glu Val Ser Gln Lys Gln Gln Glu Ser Asn Glu Lys Asp Leu 
145                 150                 155                 160 


Leu Ala Leu Asp Ala Ala Ala 
                165         


<210>  9
<211>  852
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence for gp120 backbone including restriction 
       sites

<400>  9
tcatgaaagc ttaccggtgc caccatggag tggtcttgga tcttcctgtt tctgctgagc       60

gggactgctg gagtgcattc ttccggaggg ctgtgggtca ctgtctacta tggcgtgcct      120

gtctggagag aggccaagac cacactgttc tgcgcttccg atgcaaagtc ttacgaaaaa      180

gaggtgcaca acgtctgggc cacacatgct tgcgtgccaa ctgaccccaa ccctcaggaa      240

ctggtgctga agaatgtcac cgagaacttt aatatgtgga aaaatgacat ggtggatcag      300

atgcacgagg atatcattag tctgtgggac cagtcactga agccctgcgt gaaactgaca      360

cctctgtgcg tcactctgaa ctgtagcgat gcaaaggtga acattaatgc cacatacaat      420

ggcactcgcg aggaaatcaa aaactgttcc ttcaatgcaa ctaccgaact gagggacaag      480

aagaagaagg agtacgccct gttttatcgc ctggacatcg tgcccctgaa caaggaaggg      540

aacaataaca gtgagtatcg gctgattaac tgcaatacca gcgtgattac ccaggcctgt      600

cctaaagtca ccttcgatcc aattcccatc cactactgcg caccagccgg atatgctatt      660

ctgaagtgta acaacaaaac ttttaacggg accggaccct gcaataacgt gtctacagtc      720

cagtgtactc atggcatcaa gcctgtggtc tcaacccagc tgctgctgaa tgggagcctg      780

gccgaggaag agatcattat cagaagcgag aacctgaccg acaatgtgaa gacaattatc      840

gtccacctga ac                                                          852


<210>  10
<211>  513
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence for gp120 backbone including restriction 
       sites

<400>  10

Met Glu Thr Glu Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Thr 
1               5                   10                  15      


Ala Gly Val His Ser Ser Gly Gly Leu Trp Val Thr Val Tyr Tyr Gly 
            20                  25                  30          


Val Pro Val Trp Arg Glu Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp 
        35                  40                  45              


Ala Lys Ser Tyr Glu Lys Glu Val His Asn Val Trp Ala Thr His Ala 
    50                  55                  60                  


Cys Val Pro Thr Asp Pro Asn Pro Gln Glu Leu Val Leu Lys Asn Val 
65                  70                  75                  80  


Thr Glu Asn Phe Asn Met Glu Thr Trp Lys Asn Asp Met Glu Thr Val 
                85                  90                  95      


Asp Gln Met Glu Thr His Glu Asp Ile Ile Ser Leu Trp Asp Gln Ser 
            100                 105                 110         


Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu Asn Cys 
        115                 120                 125             


Ser Asp Ala Lys Val Asn Ile Asn Ala Thr Tyr Asn Gly Thr Arg Glu 
    130                 135                 140                 


Glu Ile Lys Asn Cys Ser Phe Asn Ala Thr Thr Glu Leu Arg Asp Lys 
145                 150                 155                 160 


Lys Lys Lys Glu Tyr Ala Leu Phe Tyr Arg Leu Asp Ile Val Pro Leu 
                165                 170                 175     


Asn Lys Glu Gly Asn Asn Asn Ser Glu Tyr Arg Leu Ile Asn Cys Asn 
            180                 185                 190         


Thr Ser Val Ile Thr Gln Ala Cys Pro Lys Val Thr Phe Asp Pro Ile 
        195                 200                 205             


Pro Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn 
    210                 215                 220                 


Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr Val 
225                 230                 235                 240 


Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu 
                245                 250                 255     


Asn Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn Leu 
            260                 265                 270         


Thr Asp Asn Val Lys Thr Ile Ile Val His Leu Asn Glu Ser Val Glu 
        275                 280                 285             


Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg Ile 
    290                 295                 300                 


Gly Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile 
305                 310                 315                 320 


Arg Gln Ala His Cys Asn Ile Ser Glu Ile Lys Trp Glu Lys Thr Leu 
                325                 330                 335     


Gln Arg Val Ser Glu Lys Leu Arg Glu His Phe Asn Lys Thr Ile Ile 
            340                 345                 350         


Phe Asn Gln Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe 
        355                 360                 365             


Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Thr Ser Asp Leu Phe Phe 
    370                 375                 380                 


Asn Lys Thr Phe Asp Glu Thr Tyr Ser Thr Gly Ser Asn Ser Thr Asn 
385                 390                 395                 400 


Ser Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Glu 
                405                 410                 415     


Thr Trp Gln Glu Val Gly Arg Ala Met Glu Thr Tyr Ala Ser Pro Ile 
            420                 425                 430         


Ala Gly Glu Ile Thr Cys Lys Ser Asn Ile Thr Gly Leu Leu Leu Thr 
        435                 440                 445             


Arg Asp Gly Gly Gly Asn Asn Ser Thr Glu Glu Thr Phe Arg Pro Gly 
    450                 455                 460                 


Gly Gly Asn Met Glu Thr Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys 
465                 470                 475                 480 


Tyr Lys Val Val Glu Val Lys Pro Leu Gly Ile Ala Pro Thr Glu Ala 
                485                 490                 495     


Arg Arg Arg Val Val Gln Gln Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            500                 505                 510         


Ser 
    


<210>  11
<211>  2018
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence of CAP 256 SU gp140NFL

<400>  11
tcatgaaagc ttaccggtgc caccatggag tggtcttgga tcttcctgtt tctgctgagc       60

gggactgctg gagtgcattc ttccggaggg ctgtgggtca ctgtctacta tggcgtgcct      120

gtctggagag aggccaagac cacactgttc tgcgcttccg atgcaaagtc ttacgaaaaa      180

gaggtgcaca acgtctgggc cacacatgct tgcgtgccaa ctgaccccaa ccctcaggaa      240

ctggtgctga agaatgtcac cgagaacttt aatatgtgga aaaatgacat ggtggatcag      300

atgcacgagg atatcattag tctgtgggac cagtcactga agccctgcgt gaaactgaca      360

cctctgtgcg tcactctgaa ctgtagcgat gcaaaggtga acattaatgc cacatacaat      420

ggcactcgcg aggaaatcaa aaactgttcc ttcaatgcaa ctaccgaact gagggacaag      480

aagaagaagg agtacgccct gttttatcgc ctggacatcg tgcccctgaa caaggaaggg      540

aacaataaca gtgagtatcg gctgattaac tgcaatacca gcgtgattac ccaggcctgt      600

cctaaagtca ccttcgatcc aattcccatc cactactgcg caccagccgg atatgctatt      660

ctgaagtgta acaacaaaac ttttaacggg accggaccct gcaataacgt gtctacagtc      720

cagtgtactc atggcatcaa gcctgtggtc tcaacccagc tgctgctgaa tgggagcctg      780

gccgaggaag agatcattat cagaagcgag aacctgaccg acaatgtgaa gacaattatc      840

gtccacctga acgaatccgt ggagattaat tgcaccaggc caaacaacaa cacacgaaaa      900

tctattcgga tcggaccagg acagaccttc tacgcaacag gggacattat cggagatatc      960

aggcaggctc attgtaacat ttctgaaatc aagtgggaga aaaccctgca gcgcgtgagt     1020

gaaaagctgc gagagcactt caacaaaaca atcatcttta atcagagctc cggcggggac     1080

ctggaaatca caactcattc attcaactgc ggaggcgagt tcttttactg taacactagc     1140

gatctgttct ttaataagac ctttgacgag acctattcca caggctcaaa cagcactaat     1200

tctaccatta cactgccatg ccgaatcaaa cagattatca acatgtggca ggaagtgggc     1260

cgggcaatgt atgccagccc cattgccgga gagatcacct gtaagtccaa tatcactgga     1320

ctgctgctga ccagagatgg gggaggcaac aattctactg aagagacctt taggcccggg     1380

ggaggcaaca tgagagacaa ttggaggagc gaactgtaca agtataaagt ggtcgaggtg     1440

aagcctctgg gaatcgcacc aaccgaggcc cggagaaggg tggtccagca gggcggtgga     1500

ggctcaggtg gaggcggatc cgctgtggtc ggactgggag cagtgttcct ggggtttctg     1560

ggaactgctg gcagcaccat gggagccgct tccattactc tgaccgtgca ggcacgccag     1620

ctgctgtctg gcatcgtcca gcagcagagt aacctgctgc gggctcctga agcacagcag     1680

catatgctgc agctgaccgt gtgggggatt aagcagctgc aggcccgggt cctggctatc     1740

gagagatacc tgaaggatca gcagctgctg gggatgtggg gatgcagtgg caaactgatt     1800

tgcaccacaa acgtgtactg gaacagcagc tggtccaaca agacatataa tgaaatctgg     1860

gacaacatga cttggatgca gtgggaccgc gagatcgata actacacaga cactatctat     1920

aaactgctgg aagtctcaca gaaacagcag gagtcaaatg aaaaggacct gctggcactg     1980

gatgcggccg catgattttt ctgaattcta gactcgag                             2018


<210>  12
<211>  656
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of CAP256 SU gp140NFL

<400>  12

Met Glu Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Thr Ala Gly 
1               5                   10                  15      


Val His Ser Ser Gly Gly Leu Trp Val Thr Val Tyr Tyr Gly Val Pro 
            20                  25                  30          


Val Trp Arg Glu Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys 
        35                  40                  45              


Ser Tyr Glu Lys Glu Val His Asn Val Trp Ala Thr His Ala Cys Val 
    50                  55                  60                  


Pro Thr Asp Pro Asn Pro Gln Glu Leu Val Leu Lys Asn Val Thr Glu 
65                  70                  75                  80  


Asn Phe Asn Met Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp 
                85                  90                  95      


Ile Ile Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr 
            100                 105                 110         


Pro Leu Cys Val Thr Leu Asn Cys Ser Asp Ala Lys Val Asn Ile Asn 
        115                 120                 125             


Ala Thr Tyr Asn Gly Thr Arg Glu Glu Ile Lys Asn Cys Ser Phe Asn 
    130                 135                 140                 


Ala Thr Thr Glu Leu Arg Asp Lys Lys Lys Lys Glu Tyr Ala Leu Phe 
145                 150                 155                 160 


Tyr Arg Leu Asp Ile Val Pro Leu Asn Lys Glu Gly Asn Asn Asn Ser 
                165                 170                 175     


Glu Tyr Arg Leu Ile Asn Cys Asn Thr Ser Val Ile Thr Gln Ala Cys 
            180                 185                 190         


Pro Lys Val Thr Phe Asp Pro Ile Pro Ile His Tyr Cys Ala Pro Ala 
        195                 200                 205             


Gly Tyr Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly 
    210                 215                 220                 


Pro Cys Asn Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro 
225                 230                 235                 240 


Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu 
                245                 250                 255     


Ile Ile Ile Arg Ser Glu Asn Leu Thr Asp Asn Val Lys Thr Ile Ile 
            260                 265                 270         


Val His Leu Asn Glu Ser Val Glu Ile Asn Cys Thr Arg Pro Asn Asn 
        275                 280                 285             


Asn Thr Arg Lys Ser Ile Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala 
    290                 295                 300                 


Thr Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Ile Ser 
305                 310                 315                 320 


Glu Ile Lys Trp Glu Lys Thr Leu Gln Arg Val Ser Glu Lys Leu Arg 
                325                 330                 335     


Glu His Phe Asn Lys Thr Ile Ile Phe Asn Gln Ser Ser Gly Gly Asp 
            340                 345                 350         


Leu Glu Ile Thr Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr 
        355                 360                 365             


Cys Asn Thr Ser Asp Leu Phe Phe Asn Lys Thr Phe Asp Glu Thr Tyr 
    370                 375                 380                 


Ser Thr Gly Ser Asn Ser Thr Asn Ser Thr Ile Thr Leu Pro Cys Arg 
385                 390                 395                 400 


Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Met Tyr 
                405                 410                 415     


Ala Ser Pro Ile Ala Gly Glu Ile Thr Cys Lys Ser Asn Ile Thr Gly 
            420                 425                 430         


Leu Leu Leu Thr Arg Asp Gly Gly Gly Asn Asn Ser Thr Glu Glu Thr 
        435                 440                 445             


Phe Arg Pro Gly Gly Gly Asn Met Arg Asp Asn Trp Arg Ser Glu Leu 
    450                 455                 460                 


Tyr Lys Tyr Lys Val Val Glu Val Lys Pro Leu Gly Ile Ala Pro Thr 
465                 470                 475                 480 


Glu Ala Arg Arg Arg Val Val Gln Gln Gly Gly Gly Gly Ser Gly Gly 
                485                 490                 495     


Gly Gly Ser Ala Val Val Gly Leu Gly Ala Val Phe Leu Gly Phe Leu 
            500                 505                 510         


Gly Thr Ala Gly Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val 
        515                 520                 525             


Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu 
    530                 535                 540                 


Leu Arg Ala Pro Glu Ala Gln Gln His Met Leu Gln Leu Thr Val Trp 
545                 550                 555                 560 


Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Ile Glu Arg Tyr Leu 
                565                 570                 575     


Lys Asp Gln Gln Leu Leu Gly Met Trp Gly Cys Ser Gly Lys Leu Ile 
            580                 585                 590         


Cys Thr Thr Asn Val Tyr Trp Asn Ser Ser Trp Ser Asn Lys Thr Tyr 
        595                 600                 605             


Asn Glu Ile Trp Asp Asn Met Thr Trp Met Gln Trp Asp Arg Glu Ile 
    610                 615                 620                 


Asp Asn Tyr Thr Asp Thr Ile Tyr Lys Leu Leu Glu Val Ser Gln Lys 
625                 630                 635                 640 


Gln Gln Glu Ser Asn Glu Lys Asp Leu Leu Ala Leu Asp Ala Ala Ala 
                645                 650                 655     


<210>  13
<211>  192
<212>  DNA
<213>  Human Immunodeficiency virus

<400>  13
atgacagtga cggggacatg gaggaattat caacaatggt ggatatgggg aatcttaggc       60

ttttggatgc taatgatttg taatggcttg tgggttacag tctactatgg ggtacctgtg      120

tggagagaag caaaaactac tctattttgt gcctcagacg ctaaatcata tgagaaagag      180

gtgcataatg tc                                                          192


<210>  14
<211>  853
<212>  PRT
<213>  Human immunodeficiency virus

<400>  14

Met Thr Val Thr Gly Thr Trp Arg Asn Tyr Gln Gln Trp Trp Ile Trp 
1               5                   10                  15      


Gly Ile Leu Gly Phe Trp Met Leu Met Ile Cys Asn Gly Leu Trp Val 
            20                  25                  30          


Thr Val Tyr Tyr Gly Val Pro Val Trp Arg Glu Ala Lys Thr Thr Leu 
        35                  40                  45              


Phe Cys Ala Ser Asp Ala Lys Ser Tyr Glu Lys Glu Val His Asn Val 
    50                  55                  60                  


Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln Glu Leu 
65                  70                  75                  80  


Val Leu Lys Asn Val Thr Glu Asn Phe Asn Met Trp Lys Asn Asp Met 
                85                  90                  95      


Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp Gln Ser Leu 
            100                 105                 110         


Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu Asn Cys Ser 
        115                 120                 125             


Asp Ala Lys Val Asn Ile Asn Ala Thr Tyr Asn Gly Thr Arg Glu Glu 
    130                 135                 140                 


Ile Lys Asn Cys Ser Phe Asn Ala Thr Thr Glu Leu Arg Asp Lys Lys 
145                 150                 155                 160 


Lys Lys Glu Tyr Ala Leu Phe Tyr Arg Leu Asp Ile Val Pro Leu Asn 
                165                 170                 175     


Lys Glu Gly Asn Asn Asn Ser Glu Tyr Arg Leu Ile Asn Cys Asn Thr 
            180                 185                 190         


Ser Val Ile Thr Gln Ala Cys Pro Lys Val Thr Phe Asp Pro Ile Pro 
        195                 200                 205             


Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn 
    210                 215                 220                 


Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr Val Gln 
225                 230                 235                 240 


Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn 
                245                 250                 255     


Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr 
            260                 265                 270         


Asp Asn Val Lys Thr Ile Ile Val His Leu Asn Glu Ser Val Glu Ile 
        275                 280                 285             


Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg Ile Gly 
    290                 295                 300                 


Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg 
305                 310                 315                 320 


Gln Ala His Cys Asn Ile Ser Glu Ile Lys Trp Glu Lys Thr Leu Gln 
                325                 330                 335     


Arg Val Ser Glu Lys Leu Arg Glu His Phe Asn Lys Thr Ile Ile Phe 
            340                 345                 350         


Asn Gln Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe Asn 
        355                 360                 365             


Cys Gly Gly Glu Phe Phe Tyr Cys Asn Thr Ser Asp Leu Phe Phe Asn 
    370                 375                 380                 


Lys Thr Phe Asp Glu Thr Tyr Ser Thr Gly Ser Asn Ser Thr Asn Ser 
385                 390                 395                 400 


Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln 
                405                 410                 415     


Glu Val Gly Arg Ala Met Tyr Ala Ser Pro Ile Ala Gly Glu Ile Thr 
            420                 425                 430         


Cys Lys Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Gly 
        435                 440                 445             


Asn Asn Ser Thr Glu Glu Thr Phe Arg Pro Gly Gly Gly Asn Met Arg 
    450                 455                 460                 


Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Glu Val Lys 
465                 470                 475                 480 


Pro Leu Gly Ile Ala Pro Thr Glu Ala Arg Arg Arg Val Val Gln Lys 
                485                 490                 495     


Glu Lys Arg Ala Val Val Gly Leu Gly Ala Val Phe Leu Gly Phe Leu 
            500                 505                 510         


Gly Thr Ala Gly Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val 
        515                 520                 525             


Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu 
    530                 535                 540                 


Leu Arg Ala Ile Glu Ala Gln Gln His Met Leu Gln Leu Thr Val Trp 
545                 550                 555                 560 


Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Ile Glu Arg Tyr Leu 
                565                 570                 575     


Lys Asp Gln Gln Leu Leu Gly Met Trp Gly Cys Ser Gly Lys Leu Ile 
            580                 585                 590         


Cys Thr Thr Asn Val Tyr Trp Asn Ser Ser Trp Ser Asn Lys Thr Tyr 
        595                 600                 605             


Asn Glu Ile Trp Asp Asn Met Thr Trp Met Gln Trp Asp Arg Glu Ile 
    610                 615                 620                 


Asp Asn Tyr Thr Asp Thr Ile Tyr Lys Leu Leu Glu Val Ser Gln Lys 
625                 630                 635                 640 


Gln Gln Glu Ser Asn Glu Lys Asp Leu Leu Ala Leu Asp Ser Trp Asn 
                645                 650                 655     


Asn Leu Trp Asn Trp Phe Asp Ile Ser Lys Trp Leu Trp Tyr Ile Lys 
            660                 665                 670         


Ile Phe Ile Met Ile Val Gly Gly Leu Ile Gly Leu Arg Ile Ile Phe 
        675                 680                 685             


Ala Val Leu Ser Leu Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu 
    690                 695                 700                 


Ser Phe Gln Thr Leu Thr Pro Asn Pro Arg Glu Leu Asp Arg Leu Gly 
705                 710                 715                 720 


Gly Ile Glu Glu Glu Gly Gly Glu Gln Asp Arg Asp Arg Ser Ile Arg 
                725                 730                 735     


Leu Val Ser Gly Phe Phe Ser Leu Ala Trp Asn Asp Leu Arg Ser Leu 
            740                 745                 750         


Cys Leu Phe Cys Tyr His Arg Leu Arg Asp Phe Ile Leu Ile Ala Gly 
        755                 760                 765             


Arg Ala Val Glu Leu Leu Gly Arg Ser Ser Leu Gln Gly Leu Gln Arg 
    770                 775                 780                 


Gly Trp Glu Ile Leu Lys Tyr Leu Gly Ser Leu Val Gln Tyr Trp Gly 
785                 790                 795                 800 


Leu Glu Leu Lys Lys Ser Ala Ile Asn Leu Phe Asp Thr Ile Ala Ile 
                805                 810                 815     


Ala Val Ala Glu Gly Thr Asp Arg Ile Ile Glu Glu Phe Leu Gln Arg 
            820                 825                 830         


Ile Val Arg Ala Ile Leu His Ile Pro Arg Arg Ile Arg Gln Gly Phe 
        835                 840                 845             


Glu Ala Ala Leu Gln 
    850             


<210>  15
<211>  192
<212>  DNA
<213>  Human immunodeficiency virus

<400>  15
atgacagtga cggggacatg gaggaattat caacaatggt ggatatgggg aatcttaggc       60

ttttggatgc taatgatttg taatggcttg tgggttacag tctactatgg ggtacctgtg      120

tggagagaag caaaaactac tctattttgt gcctcagacg ctaaatcata tgagaaagag      180

gtgcataatg tc                                                          192


<210>  16
<211>  499
<212>  PRT
<213>  Human immunodeficiency virus

<400>  16

Met Thr Val Thr Gly Thr Trp Arg Asn Tyr Gln Gln Trp Trp Ile Trp 
1               5                   10                  15      


Gly Ile Leu Gly Phe Trp Met Leu Met Ile Cys Asn Gly Leu Trp Val 
            20                  25                  30          


Thr Val Tyr Tyr Gly Val Pro Val Trp Arg Glu Ala Lys Thr Thr Leu 
        35                  40                  45              


Phe Cys Ala Ser Asp Ala Lys Ser Tyr Glu Lys Glu Val His Asn Val 
    50                  55                  60                  


Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln Glu Leu 
65                  70                  75                  80  


Val Leu Lys Asn Val Thr Glu Asn Phe Asn Met Trp Lys Asn Asp Met 
                85                  90                  95      


Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp Gln Ser Leu 
            100                 105                 110         


Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu Asn Cys Ser 
        115                 120                 125             


Asp Ala Lys Val Asn Ile Asn Ala Thr Tyr Asn Gly Thr Arg Glu Glu 
    130                 135                 140                 


Ile Lys Asn Cys Ser Phe Asn Ala Thr Thr Glu Leu Arg Asp Lys Lys 
145                 150                 155                 160 


Lys Lys Glu Tyr Ala Leu Phe Tyr Arg Leu Asp Ile Val Pro Leu Asn 
                165                 170                 175     


Lys Glu Gly Asn Asn Asn Ser Glu Tyr Arg Leu Ile Asn Cys Asn Thr 
            180                 185                 190         


Ser Val Ile Thr Gln Ala Cys Pro Lys Val Thr Phe Asp Pro Ile Pro 
        195                 200                 205             


Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn 
    210                 215                 220                 


Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr Val Gln 
225                 230                 235                 240 


Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn 
                245                 250                 255     


Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr 
            260                 265                 270         


Asp Asn Val Lys Thr Ile Ile Val His Leu Asn Glu Ser Val Glu Ile 
        275                 280                 285             


Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg Ile Gly 
    290                 295                 300                 


Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg 
305                 310                 315                 320 


Gln Ala His Cys Asn Ile Ser Glu Ile Lys Trp Glu Lys Thr Leu Gln 
                325                 330                 335     


Arg Val Ser Glu Lys Leu Arg Glu His Phe Asn Lys Thr Ile Ile Phe 
            340                 345                 350         


Asn Gln Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe Asn 
        355                 360                 365             


Cys Gly Gly Glu Phe Phe Tyr Cys Asn Thr Ser Asp Leu Phe Phe Asn 
    370                 375                 380                 


Lys Thr Phe Asp Glu Thr Tyr Ser Thr Gly Ser Asn Ser Thr Asn Ser 
385                 390                 395                 400 


Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln 
                405                 410                 415     


Glu Val Gly Arg Ala Met Tyr Ala Ser Pro Ile Ala Gly Glu Ile Thr 
            420                 425                 430         


Cys Lys Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Gly 
        435                 440                 445             


Asn Asn Ser Thr Glu Glu Thr Phe Arg Pro Gly Gly Gly Asn Met Arg 
    450                 455                 460                 


Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Glu Val Lys 
465                 470                 475                 480 


Pro Leu Gly Ile Ala Pro Thr Glu Ala Arg Arg Arg Val Val Gln Lys 
                485                 490                 495     


Glu Lys Arg 
            


<210>  17
<211>  1380
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence for RVFV LPH-ptGn antigen

<400>  17
accggtatgg agtggagctg gatcttcttg ttcttgctca gcggcactgc aggtgttcac       60

tccatggatc ctcaccttag gaatagacct ggaaaaggtc ataactatat tgatggaatg      120

actcaagagg atgctacttg taagccagtg acttacgctg gagcatgctc ttcatttgat      180

gttcttttgg agaagggtaa atttcctttg ttccagtctt atgcacatca caggacatta      240

ctcgaagctg ttcatgatac tattatcgct aaagcagatc caccttcctg tgatttgcaa      300

tctgctcacg gaaatccttg catgaaggaa aaacttgtga tgaaaacaca ttgtccaaac      360

gattatcaga gcgcacacta cttgaataac gatggaaaga tggctagtgt taaatgccca      420

cctaagtacg agttgacaga agattgtaac ttctgcagac aaatgactgg agcatcactt      480

aagaaaggta gttacccatt gcaagatttg ttttgtcaga gtagcgagga tgatggatca      540

aagcttaaaa caaagatgaa aggagtttgt gaagttggtg tgcaagcttt gaagaaatgc      600

gatggacagt tatctactgc acatgaagtt gtgccttttg ctgttttcaa aaactctaag      660

aaagtgtatt tggataaact cgatcttaag actgaagaga accttttgcc agattcattt      720

gtttgtttcg agcacaaagg acaatacaag ggtactatgg atagtggtca gactaaaagg      780

gaacttaagt cattcgatat cagtcaatgt cctaagatag gaggtcatgg atccaagaaa      840

tgcactggag atgctgcatt ttgttctgca tatgaatgca ctgctcagta tgctaatgca      900

tactgtagcc acgcaaacgg atccggtgtt gtgcaaattc aggtttctgg agtgtggaag      960

aaacctttgt gcgtgggtta cgagagagtt gtggttaaaa gggaactttc agctaagcct     1020

attcaaagag ttgagccatg tactacatgc atcacaaaat gtgaaccaca tggattggtg     1080

gttaggtcta ctggttttaa gatttcctct gctgttgcat gtgcttctgg agtttgcgtg     1140

actggtagcc aatccccttc tactgaaata acacttaagt atccaggaat ctcacagtca     1200

agtggaggag atattggtgt tcatatggca cacgatgatc aaagtgttag ctccaagatt     1260

gtggctcatt gtccacctca ggatccatgc cttgttcatg gatgtatagt gtgcgctcac     1320

ggtctcatta attatcaatg tcacacagca ctttctgctt aaaggcctcc cgggctcgag     1380


<210>  18
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence for RVFV LPH-ptGn antigen

<400>  18

Met Glu Thr Glu Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Thr 
1               5                   10                  15      


Ala Gly Val His Ser Met Glu Thr Asp Pro His Leu Arg Asn Arg Pro 
            20                  25                  30          


Gly Lys Gly His Asn Tyr Ile Asp Gly Met Glu Thr Thr Gln Glu Asp 
        35                  40                  45              


Ala Thr Cys Lys Pro Val Thr Tyr Ala Gly Ala Cys Ser Ser Phe Asp 
    50                  55                  60                  


Val Leu Leu Glu Lys Gly Lys Phe Pro Leu Phe Gln Ser Tyr Ala His 
65                  70                  75                  80  


His Arg Thr Leu Leu Glu Ala Val His Asp Thr Ile Ile Ala Lys Ala 
                85                  90                  95      


Asp Pro Pro Ser Cys Asp Leu Gln Ser Ala His Gly Asn Pro Cys Met 
            100                 105                 110         


Glu Thr Lys Glu Lys Leu Val Met Glu Thr Lys Thr His Cys Pro Asn 
        115                 120                 125             


Asp Tyr Gln Ser Ala His Tyr Leu Asn Asn Asp Gly Lys Met Glu Thr 
    130                 135                 140                 


Ala Ser Val Lys Cys Pro Pro Lys Tyr Glu Leu Thr Glu Asp Cys Asn 
145                 150                 155                 160 


Phe Cys Arg Gln Met Glu Thr Thr Gly Ala Ser Leu Lys Lys Gly Ser 
                165                 170                 175     


Tyr Pro Leu Gln Asp Leu Phe Cys Gln Ser Ser Glu Asp Asp Gly Ser 
            180                 185                 190         


Lys Leu Lys Thr Lys Met Glu Thr Lys Gly Val Cys Glu Val Gly Val 
        195                 200                 205             


Gln Ala Leu Lys Lys Cys Asp Gly Gln Leu Ser Thr Ala His Glu Val 
    210                 215                 220                 


Val Pro Phe Ala Val Phe Lys Asn Ser Lys Lys Val Tyr Leu Asp Lys 
225                 230                 235                 240 


Leu Asp Leu Lys Thr Glu Glu Asn Leu Leu Pro Asp Ser Phe Val Cys 
                245                 250                 255     


Phe Glu His Lys Gly Gln Tyr Lys Gly Thr Met Glu Thr Asp Ser Gly 
            260                 265                 270         


Gln Thr Lys Arg Glu Leu Lys Ser Phe Asp Ile Ser Gln Cys Pro Lys 
        275                 280                 285             


Ile Gly Gly His Gly Ser Lys Lys Cys Thr Gly Asp Ala Ala Phe Cys 
    290                 295                 300                 


Ser Ala Tyr Glu Cys Thr Ala Gln Tyr Ala Asn Ala Tyr Cys Ser His 
305                 310                 315                 320 


Ala Asn Gly Ser Gly Val Val Gln Ile Gln Val Ser Gly Val Trp Lys 
                325                 330                 335     


Lys Pro Leu Cys Val Gly Tyr Glu Arg Val Val Val Lys Arg Glu Leu 
            340                 345                 350         


Ser Ala Lys Pro Ile Gln Arg Val Glu Pro Cys Thr Thr Cys Ile Thr 
        355                 360                 365             


Lys Cys Glu Pro His Gly Leu Val Val Arg Ser Thr Gly Phe Lys Ile 
    370                 375                 380                 


Ser Ser Ala Val Ala Cys Ala Ser Gly Val Cys Val Thr Gly Ser Gln 
385                 390                 395                 400 


Ser Pro Ser Thr Glu Ile Thr Leu Lys Tyr Pro Gly Ile Ser Gln Ser 
                405                 410                 415     


Ser Gly Gly Asp Ile Gly Val His Met Glu Thr Ala His Asp Asp Gln 
            420                 425                 430         


Ser Val Ser Ser Lys Ile Val Ala His Cys Pro Pro Gln Asp Pro Cys 
        435                 440                 445             


Leu Val His Gly Cys Ile Val Cys Ala His Gly Leu Ile Asn Tyr Gln 
    450                 455                 460                 


Cys His Thr Ala Leu Ser Ala 
465                 470     


<210>  19
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer sequence of 15-0552 Forward

<400>  19
ttcttcttct tgctgattgg                                                   20


<210>  20
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer sequence of 15-0553 Reverse

<400>  20
cacagaaaac cgctcacc                                                     18


<210>  21
<211>  4
<212>  PRT
<213>  Human immunodeficiency virus

<400>  21

Arg Glu Lys Arg 
1               


<210>  22
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Flexible linker peptide (GGGGS)2

<400>  22

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


