                         SEQUENCE LISTING

<110>  Inserm
 
<120>  CHIMERIC RECEPTOR FOR USE IN WHOLE-CELL SENSORS FOR DETECTING 
       ANALYTES OF INTEREST

<130>  BIO15505 BONNET / MC

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CadC transcriptional activator

<400>  1

Met Gln Gln Pro Val Val Arg Val Gly Glu Trp Leu Val Thr Pro Ser 
1               5                   10                  15      


Ile Asn Gln Ile Ser Arg Asn Gly Arg Gln Leu Thr Leu Glu Pro Arg 
            20                  25                  30          


Leu Ile Asp Leu Leu Val Phe Phe Ala Gln His Ser Gly Glu Val Leu 
        35                  40                  45              


Ser Arg Asp Glu Leu Ile Asp Asn Val Trp Lys Arg Ser Ile Val Thr 
    50                  55                  60                  


Asn His Val Val Thr Gln Ser Ile Ser Glu Leu Arg Lys Ser Leu Lys 
65                  70                  75                  80  


Asp Asn Asp Glu Asp Ser Pro Val Tyr Ile Ala Thr Val Pro Lys Arg 
                85                  90                  95      


Gly Tyr Lys Leu Met Val Pro Val Ile Trp Tyr Ser Glu Glu Glu Gly 
            100                 105                 110         


Glu Glu Ile Met Leu Ser Ser Pro Pro Pro Ile Pro Glu Ala Val Pro 
        115                 120                 125             


Ala Thr Asp Ser Pro Ser His Ser Leu Asn Ile Gln Asn Thr Ala Thr 
    130                 135                 140                 


Pro Pro Glu Gln Ser Pro Val 
145                 150     


<210>  2
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CadC transcriptional activator DNA binding domain

<400>  2

Met Gln Gln Pro Val Val Arg Val Gly Glu Trp Leu Val Thr Pro Ser 
1               5                   10                  15      


Ile Asn Gln Ile Ser Arg Asn Gly Arg Gln Leu Thr Leu Glu Pro Arg 
            20                  25                  30          


Leu Ile Asp Leu Leu Val Phe Phe Ala Gln His Ser Gly Glu Val Leu 
        35                  40                  45              


Ser Arg Asp Glu Leu Ile Asp Asn Val Trp Lys Arg Ser Ile Val Thr 
    50                  55                  60                  


Asn His Val Val Thr Gln Ser Ile Ser Glu Leu Arg Lys Ser Leu Lys 
65                  70                  75                  80  


Asp Asn Asp Glu Asp Ser Pro Val Tyr Ile Ala Thr Val Pro Lys Arg 
                85                  90                  95      


Gly Tyr Lys Leu Met Val Pro Val Ile Trp Tyr Ser Glu Glu Glu Gly 
            100                 105                 110         


Glu Glu Ile Met Leu Ser Ser Pro Pro Pro Ile Pro Glu Ala Val Pro 
        115                 120                 125             


Ala Thr Asp Ser Pro Ser His Ser Leu Asn Ile Gln Asn Thr Ala Thr 
    130                 135                 140                 


Pro Pro Glu Gln Ser Pro Val Lys Ser Lys Arg 
145                 150                 155 


<210>  3
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane domain

<400>  3

Gly Gly Pro Gly Leu Leu Leu Leu Leu Leu Leu Leu Leu Leu Leu Leu 
1               5                   10                  15      


Leu Leu Leu Leu Gly Pro Gly Gly 
            20                  


<210>  4
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane domain

<400>  4

Phe Thr Thr Phe Trp Val Trp Phe Phe Phe Leu Leu Ser Leu Gly Ile 
1               5                   10                  15      


Cys Val Ala Leu Val Ala Phe Ser Ser Leu 
            20                  25      


<210>  5
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane domain

<400>  5

Ile Thr Leu Ile Ile Phe Gly Val Met Ala Ala Gly Val Ile Gly Thr 
1               5                   10                  15      


Ile Leu Leu Ile Ser Tyr Gly Ile 
            20                  


<210>  6
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane domain

<400>  6

Leu Leu Leu Ile Leu Leu Gly Val Leu Ala Leu Gly Val Leu Leu Thr 
1               5                   10                  15      


Leu Leu Leu Leu Leu Leu Leu Leu 
            20                  


<210>  7
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer

<400>  7

Asp Thr Arg Leu Pro Met Ser 
1               5           


<210>  8
<211>  186
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric recpetor domain

<400>  8

Met Gln Gln Pro Val Val Arg Val Gly Glu Trp Leu Val Thr Pro Ser 
1               5                   10                  15      


Ile Asn Gln Ile Ser Arg Asn Gly Arg Gln Leu Thr Leu Glu Pro Arg 
            20                  25                  30          


Leu Ile Asp Leu Leu Val Phe Phe Ala Gln His Ser Gly Glu Val Leu 
        35                  40                  45              


Ser Arg Asp Glu Leu Ile Asp Asn Val Trp Lys Arg Ser Ile Val Thr 
    50                  55                  60                  


Asn His Val Val Thr Gln Ser Ile Ser Glu Leu Arg Lys Ser Leu Lys 
65                  70                  75                  80  


Asp Asn Asp Glu Asp Ser Pro Val Tyr Ile Ala Thr Val Pro Lys Arg 
                85                  90                  95      


Gly Tyr Lys Leu Met Val Pro Val Ile Trp Tyr Ser Glu Glu Glu Gly 
            100                 105                 110         


Glu Glu Ile Met Leu Ser Ser Pro Pro Pro Ile Pro Glu Ala Val Pro 
        115                 120                 125             


Ala Thr Asp Ser Pro Ser His Ser Leu Asn Ile Gln Asn Thr Ala Thr 
    130                 135                 140                 


Pro Pro Glu Gln Ser Pro Val Lys Ser Lys Arg Gly Gly Pro Gly Leu 
145                 150                 155                 160 


Leu Leu Leu Leu Leu Leu Leu Leu Leu Leu Leu Leu Leu Leu Leu Gly 
                165                 170                 175     


Pro Gly Gly Asp Thr Arg Leu Pro Met Ser 
            180                 185     


<210>  9
<211>  162
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  promoter

<400>  9
atccattgta aacattaaat gtttatcttt tcatgatatc aacttgcgat cctgatgtgt       60

taataaaaaa cctcaagttc tcacttacag aaacttttgt gttatttcac ctaatcttta      120

ggattaatcc ttttttcgtg agtaatctta tcgccagttt gg                         162


<210>  10
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wtTM (CadC156-188)

<400>  10

Phe Thr Thr Phe Trp Val Trp Phe Phe Phe Leu Leu Ser Leu Gly Ile 
1               5                   10                  15      


Cys Val Ala Leu Val Ala Phe Ser Ser Leu Asp Thr Arg Leu Pro Met 
            20                  25                  30          


Ser 
    


<210>  11
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artTM

<400>  11

Gly Gly Pro Gly Leu Leu Leu Leu Leu Leu Leu Leu Leu Leu Leu Leu 
1               5                   10                  15      


Leu Leu Leu Leu Gly Pro Gly Gly 
            20                  


<210>  12
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO:12

<400>  12

Gly Gly Gly Ser Gly 
1               5   


<210>  13
<211>  87
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO:13

<400>  13

Met Lys Ala Leu Thr Ala Arg Gln Gln Glu Val Phe Asp Leu Ile Arg 
1               5                   10                  15      


Asp His Ile Ser Gln Thr Gly Met Pro Pro Thr Arg Ala Glu Ile Ala 
            20                  25                  30          


Gln Arg Leu Gly Phe Arg Ser Ala Ser Ser Ala Glu Glu His Leu Lys 
        35                  40                  45              


Ala Leu Ala Arg Lys Gly Val Ile Glu Ile Val Ser Gly Ala Ser Arg 
    50                  55                  60                  


Gly Ile Arg Leu Leu Gln Glu Glu Glu Glu Gly Leu Pro Leu Val Gly 
65                  70                  75                  80  


Arg Val Ala Ala Gly Glu Pro 
                85          


<210>  14
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO:14

<400>  14

Gln Glu Glu Glu Glu 
1               5   


<210>  15
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO:15

<400>  15
cgaggctctt tccgaaaata gggttgatct ttgttgtcac tggatgtacc tacatccata       60

cggtaactca caggggctgg                                                   80


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO:16

<400>  16

Asp Thr Arg Leu Pro Met Ser 
1               5           


<210>  17
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO:17

<400>  17

Gly Gly Gly Ser Gly 
1               5   


<210>  18
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO:18

<400>  18

Glu Ala Ala Ala Lys 
1               5   


