                         SEQUENCE LISTING

<110>  Technische Universitt Dresden
 
<120>  Methods and means for genetic alteration of genomes utilizing 
       designer DNA recombining enzymes

<130>  00017P0322EPWO

<150>  EP17175895.6
<151>  2017-06-14

<160>  49    

<170>  PatentIn version 3.5

<210>  1
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinase F9-1


<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa can be any naturally occurring amino acid, preferably 
       aspartic acid or glycine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa can be any naturally occurring amino acid, preferably 
       threonine or alanine

<220>
<221>  MISC_FEATURE
<222>  (22)..(22)
<223>  Xaa can be any naturally occurring amino acid, preferably glycine
       or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa is a small unpolar amino acid, preferably selected from 
       valine, leucine, isoleucine, alanine and glycine

<220>
<221>  MISC_FEATURE
<222>  (43)..(43)
<223>  Xaa is apositively charged amino acid, preferably lysine or 
       arginine

<220>
<221>  MISC_FEATURE
<222>  (60)..(60)
<223>  Xaa can be any naturally occurring amino acid, preferably 
       aspartic acid or asparagine

<220>
<221>  MISC_FEATURE
<222>  (68)..(68)
<223>  Xaa can be any naturally occurring amino acid, preferably proline
       or serine

<220>
<221>  MISC_FEATURE
<222>  (69)..(69)
<223>  Xaa is a charged  amino acid, preferably selected form aspartic 
       acid, glutamic acid, lysine or arginine

<220>
<221>  MISC_FEATURE
<222>  (81)..(81)
<223>  Xaa is a polar amino acid, preferably selected from serine, 
       threonine, cysteine, lysine or arginine

<220>
<221>  MISC_FEATURE
<222>  (84)..(84)
<223>  Xaa can be any naturally occurring amino acid, preferably alanine
       or threonine

<220>
<221>  MISC_FEATURE
<222>  (89)..(89)
<223>  Xaa can be any naturally occurring amino acid, preferably leucine
       or glutamine

<220>
<221>  MISC_FEATURE
<222>  (93)..(93)
<223>  Xaa is a small amino acid, preferably selected from cysteine, 
       serine and alanine

<220>
<221>  MISC_FEATURE
<222>  (140)..(140)
<223>  Xaa can be any naturally occurring amino acid, preferably 
       isoleucine or threonine

<220>
<221>  MISC_FEATURE
<222>  (166)..(166)
<223>  Xaa is an unpolar amino acid, preferably selected from 
       isoleucine, leucine and valine

<220>
<221>  MISC_FEATURE
<222>  (177)..(177)
<223>  Xaa is an unpolar amino acid, preferably selected from 
       isoleucine, leucine and valine

<220>
<221>  MISC_FEATURE
<222>  (182)..(182)
<223>  Xaa is an unpolar amino acid, preferably selected from 
       isoleucine, leucine and valine

<220>
<221>  misc_feature
<222>  (183)..(183)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (184)..(184)
<223>  Xaa is a postively cahrged amino acid, preferably selected from 
       lysine and arginine

<220>
<221>  MISC_FEATURE
<222>  (186)..(186)
<223>  Xaa is a polar amino acid, preferably selected from serine or 
       threonine

<220>
<221>  MISC_FEATURE
<222>  (206)..(206)
<223>  Xaa can be any naturally occurring amino acid, preferably alanine
       or threonine

<220>
<221>  MISC_FEATURE
<222>  (222)..(222)
<223>  Xaa can be any naturally occurring amino acid, preferably valine 
       or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (225)..(225)
<223>  Xaa is an unpolar amino acid, preferably selected from 
       isoleucine, leucine and valine

<220>
<221>  MISC_FEATURE
<222>  (237)..(237)
<223>  Xaa is a polar amino acid, preferably selected from serine, 
       threonine, cysteine or tyrosine

<220>
<221>  MISC_FEATURE
<222>  (239)..(239)
<223>  Xaa is an unpolar amino acid, preferably selected from 
       isoleucine, leucine, valine or phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (249)..(249)
<223>  Xaa is a small unpolar amino acid, preferably selected from 
       alanine, glycine, isoleucine, leucine and valine

<220>
<221>  MISC_FEATURE
<222>  (259)..(259)
<223>  Xaa can be any naturally occurring amino acid, preferably 
       phenylalanine or proline

<220>
<221>  MISC_FEATURE
<222>  (260)..(260)
<223>  Xaa can be any naturally occurring amino acid, preferably 
       threonine or alanine

<220>
<221>  MISC_FEATURE
<222>  (264)..(264)
<223>  Xaa can be any naturally occurring amino acid, preferably alanine
       or aspartic acid

<220>
<221>  MISC_FEATURE
<222>  (278)..(278)
<223>  Xaa can be any naturally occurring amino acid, preferably leucine
       or glutamine

<220>
<221>  MISC_FEATURE
<222>  (284)..(284)
<223>  Xaa is an unpolar amino acid, preferably selected from 
       isoleucine, leucine or valine

<220>
<221>  MISC_FEATURE
<222>  (318)..(318)
<223>  Xaa is an unpolar amino acid, preferably selected from 
       isoleucine, leucine or valine

<220>
<221>  MISC_FEATURE
<222>  (342)..(342)
<223>  Xaa can be any naturally occurring amino acid, preferably glycine
       or aspartic acid

<400>  1

Met Ser Asn Leu Gln Thr Leu His Gln Asn Leu Ser Ala Leu Leu Val 
1               5                   10                  15      


Xaa Ala Xaa Ser Asp Xaa Ala Arg Lys Asn Leu Met Asp Xaa Phe Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Glu His Thr Trp Xaa Val Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Xaa Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Xaa Xaa Asp Val Arg Asp Tyr Leu Leu His Leu Gln Ala 
65                  70                  75                  80  


Xaa Gly Leu Xaa Val Asn Thr Ile Xaa Gln His Leu Xaa Gln Leu Asn 
                85                  90                  95      


Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Gly Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             


Glu Arg Val Lys Gln Ala Leu Ala Phe Glu Arg Xaa Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Xaa Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     


Xaa Ala Arg Ile Arg Xaa Xaa Asp Ile Xaa Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Xaa Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Arg Val Thr Arg Leu Val Xaa Arg Trp 
    210                 215                 220                 


Xaa Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Xaa Leu Xaa Cys 
225                 230                 235                 240 


Arg Val Arg Arg Asn Gly Val Ala Xaa Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     


Ser Thr Xaa Xaa Leu Gln Gly Xaa Phe Ala Ala Ala His Arg Leu Ile 
            260                 265                 270         


Tyr Gly Ala Arg Asp Xaa Ser Gly Gln Arg Tyr Xaa Thr Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Ile Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Xaa Glu Ser 
305                 310                 315                 320 


Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Xaa Asp 
            340             


<210>  2
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinase F9-2


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (18)..(21)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (39)..(39)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (80)..(80)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (84)..(84)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (93)..(93)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (97)..(97)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (125)..(125)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (132)..(132)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (182)..(183)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (186)..(186)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (195)..(195)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (206)..(206)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (216)..(216)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (249)..(249)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (253)..(253)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (259)..(260)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (264)..(264)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (266)..(266)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (273)..(273)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (276)..(276)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (278)..(278)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (306)..(306)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (325)..(325)
<223>  Xaa can be any naturally occurring amino acid

<400>  2

Met Ser Xaa Leu Xaa Thr Leu Xaa Gln Asn Leu Ser Ala Xaa Leu Xaa 
1               5                   10                  15      


Asp Xaa Xaa Xaa Xaa Glu Ala Arg Lys Asn Leu Met Asp Val Xaa Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Xaa His Thr Trp Arg Val Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Glu Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu His Leu Gln Xaa 
65                  70                  75                  80  


Arg Gly Leu Xaa Val Asn Thr Ile Gln Gln His Leu Xaa Gln Leu Asn 
                85                  90                  95      


Xaa Leu His Arg Arg Ser Gly Leu Pro Arg Pro Gly Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Xaa Asp Ala Gly 
        115                 120                 125             


Glu Arg Val Xaa Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Val Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Xaa Xaa Asp Ile Xaa Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Xaa His Ile Gly Arg Thr Lys Thr Leu Val Ser Xaa Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Xaa Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 


Arg Val Arg Arg Asn Gly Val Ala Xaa Pro Ser Ala Xaa Ser Gln Leu 
                245                 250                 255     


Ser Thr Xaa Xaa Leu Gln Gly Xaa Phe Xaa Ala Ala His Arg Leu Ile 
            260                 265                 270         


Xaa Gly Ala Xaa Asp Xaa Ser Gly Gln Arg Tyr Leu Thr Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Xaa Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Val Glu Ser 
305                 310                 315                 320 


Val Met Asn Tyr Xaa Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Gly Asp 
            340             


<210>  3
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinase F9-1a

<400>  3

Met Ser Asn Leu Gln Thr Leu His Gln Asn Leu Ser Ala Leu Leu Val 
1               5                   10                  15      


Asp Ala Thr Ser Asp Gly Ala Arg Lys Asn Leu Met Asp Val Phe Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Glu His Thr Trp Lys Val Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu His Leu Gln Ala 
65                  70                  75                  80  


Cys Gly Leu Ala Val Asn Thr Ile Leu Gln His Leu Ala Gln Leu Asn 
                85                  90                  95      


Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Gly Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             


Glu Arg Val Lys Gln Ala Leu Ala Phe Glu Arg Ile Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     


Val Ala Arg Ile Arg Ile Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Ala Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Arg Val Thr Arg Leu Val Val Arg Trp 
    210                 215                 220                 


Val Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 


Arg Val Arg Arg Asn Gly Val Ala Val Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     


Ser Thr Phe Thr Leu Gln Gly Val Phe Ala Ala Ala His Arg Leu Ile 
            260                 265                 270         


Tyr Gly Ala Arg Asp Ala Ser Gly Gln Arg Tyr Leu Thr Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Ile Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Val Glu Ser 
305                 310                 315                 320 


Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Gly Asp 
            340             


<210>  4
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinase F9-2a

<400>  4

Met Ser Lys Leu Pro Thr Leu His Gln Asn Leu Ser Ala Leu Leu Ala 
1               5                   10                  15      


Asp Ala Thr Ser Asp Glu Ala Arg Lys Asn Leu Met Asp Val Leu Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Lys His Thr Trp Arg Val Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Glu Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu His Leu Gln Thr 
65                  70                  75                  80  


Arg Gly Leu Thr Val Asn Thr Ile Gln Gln His Leu Cys Gln Leu Asn 
                85                  90                  95      


Leu Leu His Arg Arg Ser Gly Leu Pro Arg Pro Gly Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Ile Asp Ala Gly 
        115                 120                 125             


Glu Arg Val Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Val Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Val Arg Asp Ile Thr Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Ala Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 


Arg Val Arg Arg Asn Gly Val Ala Ala Pro Ser Ala Ile Ser Gln Leu 
                245                 250                 255     


Ser Thr Pro Ala Leu Gln Gly Val Phe Ala Ala Ala His Arg Leu Ile 
            260                 265                 270         


His Gly Ala Lys Asp Ala Ser Gly Gln Arg Tyr Leu Thr Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Val Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Val Glu Ser 
305                 310                 315                 320 


Val Met Asn Tyr Leu Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Gly Asp 
            340             


<210>  5
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinase F9-1b

<400>  5

Met Ser Asn Leu Gln Thr Leu His Gln Asn Leu Ser Ala Leu Leu Val 
1               5                   10                  15      


Gly Ala Ala Ser Asp Glu Ala Arg Lys Asn Leu Met Asp Ala Phe Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Glu His Thr Trp Arg Val Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Asp Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Ser Lys Asp Val Arg Asp Tyr Leu Leu His Leu Gln Ala 
65                  70                  75                  80  


Arg Gly Leu Thr Val Asn Thr Ile Gln Gln His Leu Cys Gln Leu Asn 
                85                  90                  95      


Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Gly Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             


Glu Arg Val Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Val Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Val Arg Asp Ile Thr Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Arg Val Thr Arg Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Cys Leu Ile Cys 
225                 230                 235                 240 


Arg Val Arg Arg Asn Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     


Ser Thr Pro Ala Leu Gln Gly Ile Phe Ala Ala Ala His Arg Leu Ile 
            260                 265                 270         


Tyr Gly Ala Arg Asp Asp Ser Gly Gln Arg Tyr Gln Thr Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Ile Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Ile Glu Ser 
305                 310                 315                 320 


Val Met Asn Tyr Leu Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Asp Asp 
            340             


<210>  6
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F9-2b

<400>  6

Met Ser Asn Leu Gln Thr Leu Thr Gln Asn Leu Ser Ala Ile Leu Val 
1               5                   10                  15      


Asp Gly Ala Asn Gly Glu Ala Arg Lys Asn Leu Met Asp Val Phe Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Glu His Thr Trp Arg Val Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Glu Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu His Leu Gln Ala 
65                  70                  75                  80  


Arg Gly Leu Ala Val Asn Thr Ile Gln Gln His Leu Ala Gln Leu Asn 
                85                  90                  95      


Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Gly Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             


Glu Arg Val Arg Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Val Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Ile Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Val His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Arg Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 


Arg Val Arg Arg Asn Gly Val Ala Val Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     


Ser Thr Ser Thr Leu Gln Gly Ile Phe Gly Ala Ala His Arg Leu Ile 
            260                 265                 270         


Tyr Gly Ala Gln Asp Asp Ser Gly Gln Arg Tyr Leu Thr Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Ile Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Val Glu Ser 
305                 310                 315                 320 


Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Gly Gly 
            340             


<210>  7
<211>  34
<212>  DNA
<213>  Homo sapiens

<400>  7
ctcattacat ttaaccaaaa ttatcacaat ataa                                   34


<210>  8
<211>  34
<212>  DNA
<213>  Homo sapiens

<400>  8
ccatcttttg ttagatttga atatatacat tcta                                   34


<210>  9
<211>  34
<212>  DNA
<213>  Bacteriophage P1

<400>  9
ataacttcgt ataatgtatg ctatacgaag ttat                                   34


<210>  10
<211>  34
<212>  DNA
<213>  Homo sapiens

<400>  10
atatatacgt atatagacat atatacgtat atat                                   34


<210>  11
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LoxM7 target site - artificial variant of LoxP

<400>  11
ataactctat ataatgtatg ctatatagag ttat                                   34


<210>  12
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LoxM5 target site - artificial variant of LoxP

<400>  12
ataacttcgt gcaatgtatg ctgcacgaag ttat                                   34


<210>  13
<211>  34
<212>  DNA
<213>  Human immunodeficiency virus

<400>  13
acaacatcct attacaccct atatgccaac atgg                                   34


<210>  14
<211>  34
<212>  DNA
<213>  Human immunodeficiency virus

<400>  14
aacccactgc ttaagcctca ataaagcttg cctt                                   34


<210>  15
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Intermediate target site loxF9-AL

<400>  15
ctcattacat ttaaccaaaa ttaaatgtaa tgag                                   34


<210>  16
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Intermediate target site loxF9-AR

<400>  16
ttatattgtg ataaccaaaa ttatcacaat ataa                                   34


<210>  17
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Intermediate target site loxF9-BLS

<400>  17
ccaacttttg atagatttga atatcaaaag ttgg                                   34


<210>  18
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Intermediate target site loxF9-BRS

<400>  18
tagactttat atagatttga atatataaag tcta                                   34


<210>  19
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Intermediate target site loxF9-BL

<400>  19
ccatcttttg ttagatttga ataacaaaag atgg                                   34


<210>  20
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Intermediate target site loxF9-BR

<400>  20
ccatcttttg ttagatttga ataacaaaag atgg                                   34


<210>  21
<211>  7184
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  vector pDuoF9 (source)


<220>
<221>  P1 binding site
<222>  (1412)..(1430)

<220>
<221>  Rec. F9-1 (placeholder)
<222>  (1519)..(2550)

<220>
<221>  Ribosome binding site
<222>  (2551)..(2633)

<220>
<221>  Rec. F9-2 (placeholder)
<222>  (2634)..(3665)

<220>
<221>  LoxF9a
<222>  (4220)..(4253)

<220>
<221>  LoxF9b
<222>  (4961)..(4994)

<220>
<221>  P2 binding site
<222>  (5012)..(5028)

<220>
<221>  CmR
<222>  (5315)..(5974)

<220>
<221>  Ori
<222>  (6336)..(7184)

<400>  21
gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc       60

gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat      120

ctgctcatgt ttgacagctt atcatcgatg cataatgtgc ctgtcaaatg gacgaagcag      180

ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt      240

atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg      300

ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa      360

cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc      420

tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat      480

gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc      540

tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg      600

gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta      660

tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca      720

ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata      780

ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt      840

gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca      900

gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac      960

cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat     1020

cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt     1080

atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca     1140

gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc ttttactggc     1200

tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga     1260

ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca     1320

ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag     1380

cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttt     1440

gggctagcga attcgagctc taaggaggtg ccacaattct cgagccatac ccattctaat     1500

gatcataagg aggtgtacat gtcaatacta ctgaccttgc accaaagttt gtccgcatta     1560

ctggtcgatg caacgagtga tgaggctcgc aagaacctga tggatgtgct cagggatcgc     1620

caggcgttct ccgagcgtac ctggaaagtg ctcctgtccg tttgccggac gtgggcggca     1680

tggtgcaagt tgaacaaccg gaaatggttt cccgcggaac ctgaagatgt tcgcgattac     1740

cttctacatc ttcaggctcg cggtctggca gtaaacacta tcctgcaaca tttggcccag     1800

ctaaacatgc tccaccgtcg gttcgggctg ccacgaccgg gcgacagcga cgctgtttca     1860

ctggttatgc ggcgaatccg aagggagaac gtcgatgctg gtgagcgtac gaagcaggcc     1920

ctagcgttcg agcgcactga cttcgaccag gttcgtgcac tcatggaaaa tagcgaacgg     1980

ggccaggata tacgtactct ggcacttctg ggggttgctt ataacaccct gttacgcgta     2040

tccgaaattg ccaggattcg gattaaagac atctcacgta ctgacggtgg gaggatgcta     2100

atccacatta gcagaacgaa aacgctggtc agcaccgcag gcgtagagaa ggcgcttagc     2160

ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtggc tagtgaccca     2220

aacaattacc tgttttgcca ggttagaata aatggtgtgg ccgtgccgtc tgccaccagc     2280

cggctatcaa ccgacgtcct gcgaaagatt tttgaggctg cccaccgcct gatctacggt     2340

gccaaggatg gctctggtca gagatacctg gcctggtctg ggcacagtgc ccgtgtcgga     2400

gccgcgcggg atatggcccg cgctggggtt tcaatagcgg agattatgca ggctggtggc     2460

tggaccaccg tagagagtgt catgaactat atccgtaacc tggacagtga gacaggggca     2520

atggtgcgtc tgctggaaga tggcgactag tctagaggta ccattccgat cgcggatcgg     2580

ccggaatggg atagacgtcc tatcatccac acctactagt taaggaggtg tacatgtcaa     2640

tactactgac cttgcaccaa agtttgtccg cattactggt cgatgcaacg agtgatgagg     2700

ctcgcaagaa cctgatggat gtgctcaggg atcgccaggc gttctccgag cgtacctgga     2760

aagtgctcct gtccgtttgc cggacgtggg cggcatggtg caagttgaac aaccggaaat     2820

ggtttcccgc ggaacctgaa gatgttcgcg attaccttct acatcttcag gctcgcggtc     2880

tggcagtaaa cactatcctg caacatttgg cccagctaaa catgctccac cgtcggttcg     2940

ggctgccacg accgggcgac agcgacgctg tttcactggt tatgcggcga atccgaaggg     3000

agaacgtcga tgctggtgag cgtacgaagc aggccctagc gttcgagcgc actgacttcg     3060

accaggttcg tgcactcatg gaaaatagcg aacggggcca ggatatacgt actctggcac     3120

ttctgggggt tgcttataac accctgttac gcgtatccga aattgccagg attcggatta     3180

aagacatctc acgtactgac ggtgggagga tgctaatcca cattagcaga acgaaaacgc     3240

tggtcagcac cgcaggcgta gagaaggcgc ttagcctggg ggtaactaaa ctggtcgagc     3300

gatggatttc cgtctctggt gtggctagtg acccaaacaa ttacctgttt tgccaggtta     3360

gaataaatgg tgtggccgtg ccgtctgcca ccagccggct atcaaccgac gtcctgcgaa     3420

agatttttga ggctgcccac cgcctgatct acggtgccaa ggatggctct ggtcagagat     3480

acctggcctg gtctgggcac agtgcccgtg tcggagccgc gcgggatatg gcccgcgctg     3540

gggtttcaat agcggagatt atgcaggctg gtggctggac caccgtagag agtgtcatga     3600

actatatccg taacctggac agtgagacag gggcaatggt gcgtctgctg gaagatggcg     3660

actagtctag acgtacgcct gcaggcaagc ttggctgttt tggcggatga gagaagattt     3720

tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg     3780

gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta     3840

gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata     3900

aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac     3960

gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc     4020

ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc     4080

atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca     4140

ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa     4200

aaggaagagt atgagatctc tcattacatt taaccaaaat tatcacaata taaaagcttg     4260

catgcctgca gatcgaggct ggcctgtatc ggacgggtca tctcgtttcc ttagctgtgt     4320

gcgccatgta aatggcccgg acgtgatggc gtaaatccac ggctgtagcg cgctacgctt     4380

agatcctcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt     4440

atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc     4500

cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac     4560

cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca     4620

gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag     4680

tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc     4740

agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct     4800

aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg     4860

actaattttt tttatttatg cagaggccga ggccgcctcg gcctaggaac agtcgacgac     4920

actgcagaga cctacttcac taacaaccgg tacagttcga ccatcttttg ttagatttga     4980

atatatacat tctaagatct caacatttcc gtgtcgccct tattcccttt tttgcggcat     5040

tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc     5100

agttgggtgc agcaaactat taactggcga actacttact ctagcttccc ggcaacaatt     5160

aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccggtcgaa     5220

tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag     5280

gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc     5340

agtactgttg taattcatta agcattctgc cgacatggaa gccatcacag acggcatgat     5400

gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg     5460

tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac     5520

tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg     5580

ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat     5640

cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt     5700

aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt     5760

ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct     5820

tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg     5880

tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat     5940

caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc     6000

tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac     6060

ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat     6120

caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt     6180

cggcgcaaag tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt     6240

tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg     6300

gtggtgcgta acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt     6360

actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc     6420

tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga     6480

ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga     6540

tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg     6600

tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg     6660

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg     6720

cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg     6780

tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat     6840

gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc     6900

caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg     6960

agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact     7020

gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa     7080

aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac     7140

gatctcaaga agatcatctt attaatcaga taaaatattt ctag                      7184


<210>  22
<211>  2795
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pDonor vector


<220>
<221>  AmpR
<222>  (121)..(981)

<220>
<221>  LoxF9a
<222>  (1042)..(1075)

<220>
<221>  KanR
<222>  (1172)..(1987)

<220>
<221>  LoxF9b
<222>  (2062)..(2095)

<220>
<221>  R6K-ori
<222>  (2328)..(2719)

<400>  22
tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg       60

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt      120

atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct      180

gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca      240

cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc      300

gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc      360

cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg      420

gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta      480

tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc      540

ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt      600

gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg      660

cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct      720

tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc      780

tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct      840

cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac      900

acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc      960

tcactgatta agcattggta acccagcccg cctaatgagc gggctttttt ttgaacaaaa     1020

ggctgcagct ggacttctag actcattaca tttaaccaaa attatcacaa tataaggtct     1080

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgaacaa taaaactgtc     1140

tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg     1200

ctctaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg     1260

cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc     1320

agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt     1380

cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac     1440

tcctgatgat gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt     1500

agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg     1560

gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc     1620

tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg     1680

taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc     1740

ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa     1800

attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc     1860

catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa     1920

atatggtgtt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt     1980

tttctaagaa ttaattcatg agcggataca tatttgaatg tatttagaaa aataaacaaa     2040

taggggttcc gcgcacattt cccatctttt gttagatttg aatatataca ttctactcga     2100

gggtgcgaat aagggacatg aagaaggaac acccgctcgc gggtgggcct acttcaccta     2160

tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa     2220

atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga     2280

agcagggtta tgcagcggaa aacggccacg atgcgtccgg cgtagaggat ctgaagatca     2340

gcagttcaac ctgttgatag tacgtactaa gctctcatgt ttcacgtact aagctctcat     2400

gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa cgtactaagc     2460

tctcatggct aacgtactaa gctctcatgt ttcacgtact aagctctcat gtttgaacaa     2520

taaaattaat ataaatcagc aacttaaata gcctctaagg ttttaagttt tataagaaaa     2580

aaaagaatat ataaggcttt taaagctttt aaggtttaac ggttgtggac aacaagccag     2640

ggatgtaacg cactgagaag cccttagagc ctctcaaagc aattttgagt gacacaggaa     2700

cacttaacgg ctgacatggg aattagcttc acgctgccgc aagcactcag ggcgcaaggg     2760

ctgctaaagg aagcggatag acgtcaggtg gcact                                2795


<210>  23
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer P1 for amplifying the plasmid pDuoF9

<400>  23
ctctactgtt tctccatac                                                    19


<210>  24
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer P2 for amplifying the plasmid pDuoF9

<400>  24
agggaataag ggcgaca                                                      17


<210>  25
<211>  7463
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  vector pDuoF9 product


<220>
<221>  P1 binding site
<222>  (1412)..(1430)

<220>
<221>  Rec. F9-1 (placeholder)
<222>  (1519)..(2550)

<220>
<221>  Ribosome binding site
<222>  (2551)..(2633)

<220>
<221>  Rec. F9-2 (placeholder)
<222>  (2634)..(3665)

<220>
<221>  LoxF9a
<222>  (4220)..(4253)

<220>
<221>  KanR
<222>  (4350)..(5165)

<220>
<221>  LoxF9b
<222>  (5240)..(5273)

<220>
<221>  P2 binding site
<222>  (5291)..(5307)

<220>
<221>  CmR
<222>  (5594)..(6253)

<220>
<221>  Ori
<222>  (6615)..(7463)

<400>  25
gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc       60

gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat      120

ctgctcatgt ttgacagctt atcatcgatg cataatgtgc ctgtcaaatg gacgaagcag      180

ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt      240

atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg      300

ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa      360

cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc      420

tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat      480

gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc      540

tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg      600

gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta      660

tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca      720

ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata      780

ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt      840

gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca      900

gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac      960

cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat     1020

cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt     1080

atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca     1140

gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc ttttactggc     1200

tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga     1260

ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca     1320

ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag     1380

cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttt     1440

gggctagcga attcgagctc taaggaggtg ccacaattct cgagccatac ccattctaat     1500

gatcataagg aggtgtacat gtcaatacta ctgaccttgc accaaagttt gtccgcatta     1560

ctggtcgatg caacgagtga tgaggctcgc aagaacctga tggatgtgct cagggatcgc     1620

caggcgttct ccgagcgtac ctggaaagtg ctcctgtccg tttgccggac gtgggcggca     1680

tggtgcaagt tgaacaaccg gaaatggttt cccgcggaac ctgaagatgt tcgcgattac     1740

cttctacatc ttcaggctcg cggtctggca gtaaacacta tcctgcaaca tttggcccag     1800

ctaaacatgc tccaccgtcg gttcgggctg ccacgaccgg gcgacagcga cgctgtttca     1860

ctggttatgc ggcgaatccg aagggagaac gtcgatgctg gtgagcgtac caagcaggcc     1920

ctagcgttcg agcgcactga cttcgaccag gttcgtgcac tcatggaaaa tagcgaacgg     1980

ggccaggata tacgtactct ggcacttctg ggggttgctt ataacaccct gttacgcgta     2040

tccgaaattg ccaggattcg gattaaagac atctcacgta ctgacggtgg gaggatgcta     2100

atccacatta gcagaacgaa aacgctggtc agcaccgcag gcgtagagaa ggcgcttagc     2160

ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtggc tagtgaccca     2220

aacaattacc tgttttgcca ggttagaata aatggtgtgg ccgtgccgtc tgccaccagc     2280

cggctatcaa ccgacgtcct gcgaaagatt tttgaggctg cccaccgcct gatctacggt     2340

gccaaggatg gctctggtca gagatacctg gcctggtctg ggcacagtgc ccgtgtcgga     2400

gccgcgcggg atatggcccg cgctggggtt tcaatagcgg agattatgca ggctggtggc     2460

tggaccaccg tagagagtgt catgaactat atccgtaacc tggacagtga gacaggggca     2520

atggtgcgtc tgctggaaga tggcgactag tctagaggta ccattccgat cgcggatcgg     2580

ccggaatggg atagacgtcc tatcatccac acctactagt taaggaggtg tacatgtcaa     2640

tactactgac cttgcaccaa agtttgtccg cattactggt cgatgcaacg agtgatgagg     2700

ctcgcaagaa cctgatggat gtgctcaggg atcgccaggc gttctccgag cgtacctgga     2760

aagtgctcct gtccgtttgc cggacgtggg cggcatggtg caagttgaac aaccggaaat     2820

ggtttcccgc ggaacctgaa gatgttcgcg attaccttct acatcttcag gctcgcggtc     2880

tggcagtaaa cactatcctg caacatttgg cccagctaaa catgctccac cgtcggttcg     2940

ggctgccacg accgggcgac agcgacgctg tttcactggt tatgcggcga atccgaaggg     3000

agaacgtcga tgctggtgag cgtaccaagc aggccctagc gttcgagcgc actgacttcg     3060

accaggttcg tgcactcatg gaaaatagcg aacggggcca ggatatacgt actctggcac     3120

ttctgggggt tgcttataac accctgttac gcgtatccga aattgccagg attcggatta     3180

aagacatctc acgtactgac ggtgggagga tgctaatcca cattagcaga acgaaaacgc     3240

tggtcagcac cgcaggcgta gagaaggcgc ttagcctggg ggtaactaaa ctggtcgagc     3300

gatggatttc cgtctctggt gtggctagtg acccaaacaa ttacctgttt tgccaggtta     3360

gaataaatgg tgtggccgtg ccgtctgcca ccagccggct atcaaccgac gtcctgcgaa     3420

agatttttga ggctgcccac cgcctgatct acggtgccaa ggatggctct ggtcagagat     3480

acctggcctg gtctgggcac agtgcccgtg tcggagccgc gcgggatatg gcccgcgctg     3540

gggtttcaat agcggagatt atgcaggctg gtggctggac caccgtagag agtgtcatga     3600

actatatccg taacctggac agtgagacag gggcaatggt gcgtctgctg gaagatggcg     3660

actagtctag acgtacgcct gcaggcaagc ttggctgttt tggcggatga gagaagattt     3720

tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg     3780

gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta     3840

gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata     3900

aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac     3960

gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc     4020

ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc     4080

atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca     4140

ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa     4200

aaggaagagt atgagatctc tcattacatt taaccaaaat tatcacaata taaggtctga     4260

cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgaacaata aaactgtctg     4320

cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct     4380

ctaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg     4440

ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag     4500

agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca     4560

gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc     4620

ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc caggtattag     4680

aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt     4740

tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc     4800

aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta     4860

atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg     4920

attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat     4980

taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca     5040

tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat     5100

atggtgttga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt     5160

tctaagaatt aattcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata     5220

ggggttccgc gcacatttcc catcttttgt tagatttgaa tatatacatt ctaagatctc     5280

aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc     5340

acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca gcaaactatt     5400

aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga     5460

taaagttgca ggaccacttc tgcgctcggc ccggtcgaat ttgctttcga atttctgcca     5520

ttcatccgct tattatcact tattcaggcg tagcaccagg cgtttaaggg caccaataac     5580

tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa     5640

gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc gccagcggca     5700

tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt     5760

tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga     5820

cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg     5880

ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga     5940

gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc     6000

atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca ttcatcaggc     6060

gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta     6120

aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa     6180

atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga     6240

tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc     6300

ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt     6360

ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta     6420

tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg     6480

atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct     6540

gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc     6600

accgccggac atcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg     6660

gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa     6720

tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg     6780

actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa     6840

gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc     6900

cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta     6960

taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt     7020

cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact     7080

cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc     7140

cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa     7200

agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg     7260

ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac     7320

ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt     7380

tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta     7440

ttaatcagat aaaatatttc tag                                             7463


<210>  26
<211>  800
<212>  DNA
<213>  Homo sapiens


<220>
<221>  loxF9a
<222>  (1)..(34)

<220>
<221>  loxF9b
<222>  (766)..(800)

<220>
<221>  Exon 8 of the F9 gene wildtype
<222>  (766)..(800)

<400>  26
ctcattacat ttaaccaaaa ttatcacaat ataagaatga gatctttaac attgccaatt       60

aggtcagtgg tcccaagtag tcacttagaa aatctgtgta tgtgaaatac tgtttgtgac      120

ttaaaatgaa atttattttt aataggtgaa cataatattg aggagacaga acatacagag      180

caaaagcgaa atgtgattcg aattattcct caccacaact acaatgcagc tattaataag      240

tacaaccatg acattgccct tctggaactg gacgaaccct tagtgctaaa cagctacgtt      300

acacctattt gcattgctga caaggaatac acgaacatct tcctcaaatt tggatctggc      360

tatgtaagtg gctggggaag agtcttccac aaagggagat cagctttagt tcttcagtac      420

cttagagttc cacttgttga ccgagccaca tgtcttcgat ctacaaagtt caccatctat      480

aacaacatgt tctgtgctgg cttccatgaa ggaggtagag attcatgtca aggagatagt      540

gggggacccc atgttactga agtggaaggg accagtttct taactggaat tattagctgg      600

ggtgaagagt gtgcaatgaa aggcaaatat ggaatatata ccaaggtatc ccggtatgtc      660

aactggatta aggaaaaaac aaagctcact taatgaaaga tggatttcca aggttaattc      720

attggaattg aaaattaaca gggcctctca ctaactaatc actttcccat cttttgttag      780

atttgaatat atacattcta                                                  800


<210>  27
<211>  800
<212>  DNA
<213>  Homo sapiens


<220>
<221>  loxF9a
<222>  (1)..(34)

<220>
<221>  Exon 8 of the F9 gene Arg338Leu (Padua mutation)
<222>  (146)..(693)

<220>
<221>  LoxF9b
<222>  (766)..(800)

<400>  27
ctcattacat ttaaccaaaa ttatcacaat ataagaatga gatctttaac attgccaatt       60

aggtcagtgg tcccaagtag tcacttagaa aatctgtgta tgtgaaatac tgtttgtgac      120

ttaaaatgaa atttattttt aataggtgaa cataatattg aggagacaga acatacagag      180

caaaagcgaa atgtgattcg aattattcct caccacaact acaatgcagc tattaataag      240

tacaaccatg acattgccct tctggaactg gacgaaccct tagtgctaaa cagctacgtt      300

acacctattt gcattgctga caaggaatac acgaacatct tcctcaaatt tggatctggc      360

tatgtaagtg gctggggaag agtcttccac aaagggagat cagctttagt tcttcagtac      420

cttagagttc cacttgttga ccgagccaca tgtcttctgt ctacaaagtt caccatctat      480

aacaacatgt tctgtgctgg cttccatgaa ggaggtagag attcatgtca aggagatagt      540

gggggacccc atgttactga agtggaaggg accagtttct taactggaat tattagctgg      600

ggtgaagagt gtgcaatgaa aggcaaatat ggaatatata ccaaggtatc ccggtatgtc      660

aactggatta aggaaaaaac aaagctcact taatgaaaga tggatttcca aggttaattc      720

attggaattg aaaattaaca gggcctctca ctaactaatc actttcccat cttttgttag      780

atttgaatat atacattcta                                                  800


<210>  28
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinase F9-1


<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (60)..(60)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (68)..(69)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (84)..(84)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (89)..(89)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (93)..(93)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (140)..(140)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (166)..(166)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (177)..(177)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (182)..(183)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (186)..(186)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (206)..(206)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (222)..(222)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (225)..(225)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (237)..(237)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (239)..(239)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (249)..(249)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (259)..(260)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (264)..(264)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (278)..(278)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (284)..(284)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (318)..(318)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (342)..(342)
<223>  Xaa can be any naturally occurring amino acid

<400>  28

Met Ser Asn Leu Gln Thr Leu His Gln Asn Leu Ser Ala Leu Leu Val 
1               5                   10                  15      


Xaa Ala Xaa Ser Asp Xaa Ala Arg Lys Asn Leu Met Asp Xaa Phe Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Glu His Thr Trp Xaa Val Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Xaa Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Xaa Xaa Asp Val Arg Asp Tyr Leu Leu His Leu Gln Ala 
65                  70                  75                  80  


Xaa Gly Leu Xaa Val Asn Thr Ile Xaa Gln His Leu Xaa Gln Leu Asn 
                85                  90                  95      


Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Gly Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             


Glu Arg Val Lys Gln Ala Leu Ala Phe Glu Arg Xaa Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Xaa Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     


Xaa Ala Arg Ile Arg Xaa Xaa Asp Ile Xaa Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Xaa Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Arg Val Thr Arg Leu Val Xaa Arg Trp 
    210                 215                 220                 


Xaa Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Xaa Leu Xaa Cys 
225                 230                 235                 240 


Arg Val Arg Arg Asn Gly Val Ala Xaa Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     


Ser Thr Xaa Xaa Leu Gln Gly Xaa Phe Ala Ala Ala His Arg Leu Ile 
            260                 265                 270         


Tyr Gly Ala Arg Asp Xaa Ser Gly Gln Arg Tyr Xaa Thr Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Ile Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Xaa Glu Ser 
305                 310                 315                 320 


Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Xaa Asp 
            340             


<210>  29
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinase F9-1


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (18)..(21)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (39)..(39)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (80)..(80)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (84)..(84)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (93)..(93)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (97)..(97)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (125)..(125)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (132)..(132)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (182)..(183)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (186)..(186)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (195)..(195)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (206)..(206)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (216)..(216)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (249)..(249)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (253)..(253)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (259)..(260)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (264)..(264)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (266)..(266)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (273)..(273)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (276)..(276)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (278)..(278)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (306)..(306)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (325)..(325)
<223>  Xaa can be any naturally occurring amino acid

<400>  29

Met Ser Xaa Leu Xaa Thr Leu Xaa Gln Asn Leu Ser Ala Xaa Leu Xaa 
1               5                   10                  15      


Asp Xaa Xaa Xaa Xaa Glu Ala Arg Lys Asn Leu Met Asp Val Xaa Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Xaa His Thr Trp Arg Val Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Glu Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu His Leu Gln Xaa 
65                  70                  75                  80  


Arg Gly Leu Xaa Val Asn Thr Ile Gln Gln His Leu Xaa Gln Leu Asn 
                85                  90                  95      


Xaa Leu His Arg Arg Ser Gly Leu Pro Arg Pro Gly Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Xaa Asp Ala Gly 
        115                 120                 125             


Glu Arg Val Xaa Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Val Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Xaa Xaa Asp Ile Xaa Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Xaa His Ile Gly Arg Thr Lys Thr Leu Val Ser Xaa Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Xaa Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 


Arg Val Arg Arg Asn Gly Val Ala Xaa Pro Ser Ala Xaa Ser Gln Leu 
                245                 250                 255     


Ser Thr Xaa Xaa Leu Gln Gly Xaa Phe Xaa Ala Ala His Arg Leu Ile 
            260                 265                 270         


Xaa Gly Ala Xaa Asp Xaa Ser Gly Gln Arg Tyr Leu Thr Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Xaa Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Val Glu Ser 
305                 310                 315                 320 


Val Met Asn Tyr Xaa Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Gly Asp 
            340             


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer P1# for amplifying the plasmid pDuoF9

<400>  30
cggcgtcaca ctttgctatg                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer P2# for amplifying the plasmid pDuoF9

<400>  31
cccttaaacg cctggtgcta                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer P3 for amplifying the recombinase library

<400>  32
aagattagcg gatcctacct                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer P4 for amplifying the recombinase library

<400>  33
gtgattagtt agtgagaggc                                                   20


<210>  34
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinase R#1

<400>  34

Met Ser Lys Leu Gln Thr Ile His Gln Asn Leu Ser Ala Leu Leu Val 
1               5                   10                  15      


Asp Ala Thr Ser Asp Glu Ala Arg Lys Asn Leu Met Asp Val Leu Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Lys His Thr Trp Arg Val Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Glu Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu His Leu Gln Thr 
65                  70                  75                  80  


Arg Gly Leu Thr Val Asn Thr Ile Gln Gln His Leu Cys Gln Leu Asn 
                85                  90                  95      


Leu Leu His Arg Arg Ser Gly Leu Pro Arg Pro Gly Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Ile Asp Ala Gly 
        115                 120                 125             


Glu Arg Val Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Val Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Val Arg Asp Ile Thr Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Ala Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 


Arg Val Arg Arg Asn Gly Val Ala Ala Pro Ser Ala Ile Ser Gln Leu 
                245                 250                 255     


Ser Thr Pro Ala Leu Gln Gly Val Phe Ala Ala Ala His Arg Leu Ile 
            260                 265                 270         


His Gly Ala Lys Asp Ala Ser Gly Gln Arg Tyr Leu Thr Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Val Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Val Glu Ser 
305                 310                 315                 320 


Val Met Asn Tyr Leu Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Gly Asp 
            340             


<210>  35
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinase R#7-B5

<400>  35

Met Ser Lys Leu Gln Thr Ile His Gln Asp Leu Ser Ala Leu Leu Val 
1               5                   10                  15      


Asp Val Thr Ser Asp Glu Ala Arg Lys Asn Leu Met Asp Val Leu Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Lys His Thr Trp Arg Val Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Glu Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu His Leu Gln Thr 
65                  70                  75                  80  


Arg Gly Leu Thr Val Asn Thr Ile Gln Gln His Leu Cys Gln Leu Asn 
                85                  90                  95      


Leu Leu His Arg Arg Ser Gly Leu Pro Arg Pro Gly Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Ile Asp Ala Gly 
        115                 120                 125             


Glu Arg Val Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Val Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Val Arg Asp Ile Thr Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Ala Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn His Leu Phe Cys 
225                 230                 235                 240 


Arg Val Arg Arg Asn Gly Val Ala Ala Pro Ser Ala Ile Ser Gln Leu 
                245                 250                 255     


Ser Thr Pro Ala Leu Gln Gly Val Phe Ala Ala Ala His Arg Leu Ile 
            260                 265                 270         


His Gly Ala Lys Asp Ala Ser Gly Gln Arg Tyr Leu Thr Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Val Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Val Glu Ser 
305                 310                 315                 320 


Val Met Asn Tyr Leu Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Gly Asp 
            340             


<210>  36
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinase F9-3

<400>  36

Met Pro Lys Leu Gln Thr Ile His Gln Asp Leu Ser Ala Leu Leu Val 
1               5                   10                  15      


Asp Val Thr Ser Asp Glu Ala Arg Lys Asn Leu Met Asp Val Leu Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Arg His Thr Trp Arg Val Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Glu Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu Gln Leu Gln Thr 
65                  70                  75                  80  


Arg Gly Leu Thr Val Asn Thr Ile Gln Gln His Leu Cys Gln Leu Asn 
                85                  90                  95      


Leu Leu His Arg Arg Ser Gly Leu Pro Arg Pro Gly Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Ile Asp Ala Gly 
        115                 120                 125             


Glu Arg Val Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Val Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Val Arg Asp Ile Thr Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Ile Thr Lys Thr Leu Val Ser Ala Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn His Leu Phe Cys 
225                 230                 235                 240 


Arg Val Arg Arg Asn Gly Val Ala Ala Pro Ser Ala Ile Ser Gln Leu 
                245                 250                 255     


Ser Thr Pro Ala Leu Gln Gly Val Phe Ala Ala Ala His Arg Leu Ile 
            260                 265                 270         


His Gly Ala Lys Asp Asp Ser Gly Gln Arg Tyr Leu Thr Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Pro Val Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Val Glu Ser 
305                 310                 315                 320 


Val Met Ser Tyr Leu Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Gly Asp 
            340             


<210>  37
<211>  4930
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pDonor-ex8


<220>
<221>  Origin of replication
<222>  (167)..(786)

<220>
<221>  AmpR
<222>  (941)..(1801)

<220>
<221>  KanR
<222>  (1822)..(2610)

<220>
<221>  part of human F9 gene including exon 8
<222>  (3638)..(4636)

<220>
<221>  LoxF9a
<222>  (3713)..(3746)

<220>
<221>  LoxF9b
<222>  (4479)..(4512)

<220>
<221>  P4 binding site
<222>  (4479)..(4512)

<400>  37
cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat       60

cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga      120

acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt      180

ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt      240

ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc      300

gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa      360

gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct      420

ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta      480

actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg      540

gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc      600

ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta      660

ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg      720

gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt      780

tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg      840

tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta      900

aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg      960

aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg     1020

tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc     1080

gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg     1140

agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg     1200

aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag     1260

gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat     1320

caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc     1380

cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc     1440

ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa     1500

ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac     1560

gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt     1620

cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc     1680

gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa     1740

caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca     1800

tactcttcct ttttcaattc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg     1860

cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag     1920

ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag     1980

ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca     2040

ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc     2100

gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag     2160

accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg     2220

gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt     2280

ctcggcagga gcaaggtggg atgacaggag atcctgcccc ggcacttcgc ccaatagcag     2340

ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt     2400

ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc     2460

ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga     2520

gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg     2580

agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg     2640

atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac     2700

tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc     2760

tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt     2820

tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg     2880

tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg     2940

ccctgaattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa     3000

taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt     3060

gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg     3120

cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt     3180

ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga     3240

gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg     3300

ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg     3360

cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag     3420

ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa     3480

ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca     3540

gtgaattgta atacgactca ctatagggcg aattgggccc tctagatgca tgctcgagcg     3600

gccgccagtg tgatggatat ctgcagaatt cgccctttaa cagcatgagt gaacagaacc     3660

atctctatga tagtcctgaa tggctttttg gtctgaaaaa tatgcattgg ctctcattac     3720

atttaaccaa aattatcaca atataagaat gagatcttta acattgccaa ttaggtcagt     3780

ggtcccaagt agtcacttag aaaatctgtg tatgtgaaat actgtttgtg acttaaaatg     3840

aaatttattt ttaataggtg aacataatat tgaggagaca gaacatacag agcaaaagcg     3900

aaatgtgatt cgaattattc ctcaccacaa ctacaatgca gctattaata agtacaacca     3960

tgacattgcc cttctggaac tggacgaacc cttagtgcta aacagctacg ttacacctat     4020

ttgcattgct gacaaggaat acacgaacat cttcctcaaa tttggatctg gctatgtaag     4080

tggctgggga agagtcttcc acaaagggag atcagcttta gttcttcagt accttagagt     4140

tccacttgtt gaccgagcca catgtcttcg atctacaaag ttcaccatct ataacaacat     4200

gttctgtgct ggcttccatg aaggaggtag agattcatgt caaggagata gtgggggacc     4260

ccatgttact gaagtggaag ggaccagttt cttaactgga attattagct ggggtgaaga     4320

gtgtgcaatg aaaggcaaat atggaatata taccaaggta tcccggtatg tcaactggat     4380

taaggaaaaa acaaagctca cttaatgaaa gatggatttc caaggttaat tcattggaat     4440

tgaaaattaa cagggcctct cactaactaa tcactttccc atcttttgtt agatttgaat     4500

atatacattc tatgatcatt gctttttctc tttacagggg agaatttcat attttacctg     4560

agcaaattga ttagaaaatg gaaccactag aggaatataa tgtgttagga aattacagtc     4620

atttctaagg gcccagaagg gcgaattcca gcacactggc ggccgttact agtggatccg     4680

agctcggtac caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat     4740

ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc     4800

taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga     4860

aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt     4920

attgggcgct                                                            4930


<210>  38
<211>  6996
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pDuoF9-ex8 (outcome)


<220>
<221>  P3 binding site
<222>  (1373)..(1392)

<220>
<221>  Recombinase R1 (placeholder)
<222>  (1519)..(2550)

<220>
<221>  Recombinase R#7-B5 (placeholder)
<222>  (1519)..(2550)

<220>
<221>  Ribosome binding site
<222>  (2551)..(2633)

<220>
<221>  LoxF9a
<222>  (4220)..(4253)

<220>
<221>  exchanged part of human F9 gene including exon 8
<222>  (4220)..(4253)

<220>
<221>  LoxF9b
<222>  (4962)..(5019)

<220>
<221>  P4 binding site
<222>  (4962)..(4981)

<220>
<221>  CmR
<222>  (5127)..(5786)

<220>
<221>  Origin of replication
<222>  (6148)..(6996)

<400>  38
gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc       60

gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat      120

ctgctcatgt ttgacagctt atcatcgatg cataatgtgc ctgtcaaatg gacgaagcag      180

ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt      240

atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg      300

ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa      360

cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc      420

tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat      480

gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc      540

tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg      600

gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta      660

tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca      720

ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata      780

ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt      840

gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca      900

gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac      960

cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat     1020

cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt     1080

atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca     1140

gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc ttttactggc     1200

tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga     1260

ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca     1320

ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag     1380

cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttt     1440

gggctagcga attcgagctc taaggaggtg ccacaattct cgagccatac ccattctaat     1500

gatcataagg aggtgtacat gtcaatacta ctgaccttgc accaaagttt gtccgcatta     1560

ctggtcgatg caacgagtga tgaggctcgc aagaacctga tggatgtgct cagggatcgc     1620

caggcgttct ccgagcgtac ctggaaagtg ctcctgtccg tttgccggac gtgggcggca     1680

tggtgcaagt tgaacaaccg gaaatggttt cccgcggaac ctgaagatgt tcgcgattac     1740

cttctacatc ttcaggctcg cggtctggca gtaaacacta tcctgcaaca tttggcccag     1800

ctaaacatgc tccaccgtcg gttcgggctg ccacgaccgg gcgacagcga cgctgtttca     1860

ctggttatgc ggcgaatccg aagggagaac gtcgatgctg gtgagcgtac caagcaggcc     1920

ctagcgttcg agcgcactga cttcgaccag gttcgtgcac tcatggaaaa tagcgaacgg     1980

ggccaggata tacgtactct ggcacttctg ggggttgctt ataacaccct gttacgcgta     2040

tccgaaattg ccaggattcg gattaaagac atctcacgta ctgacggtgg gaggatgcta     2100

atccacatta gcagaacgaa aacgctggtc agcaccgcag gcgtagagaa ggcgcttagc     2160

ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtggc tagtgaccca     2220

aacaattacc tgttttgcca ggttagaata aatggtgtgg ccgtgccgtc tgccaccagc     2280

cggctatcaa ccgacgtcct gcgaaagatt tttgaggctg cccaccgcct gatctacggt     2340

gccaaggatg gctctggtca gagatacctg gcctggtctg ggcacagtgc ccgtgtcgga     2400

gccgcgcggg atatggcccg cgctggggtt tcaatagcgg agattatgca ggctggtggc     2460

tggaccaccg tagagagtgt catgaactat atccgtaacc tggacagtga gacaggggca     2520

atggtgcgtc tgctggaaga tggcgactag tctagaggta ccattccgat cgcggatcgg     2580

ccggaatggg atagacgtcc tatcatccac acctactagt taaggaggtg tacatgtcaa     2640

tactactgac cttgcaccaa agtttgtccg cattactggt cgatgcaacg agtgatgagg     2700

ctcgcaagaa cctgatggat gtgctcaggg atcgccaggc gttctccgag cgtacctgga     2760

aagtgctcct gtccgtttgc cggacgtggg cggcatggtg caagttgaac aaccggaaat     2820

ggtttcccgc ggaacctgaa gatgttcgcg attaccttct acatcttcag gctcgcggtc     2880

tggcagtaaa cactatcctg caacatttgg cccagctaaa catgctccac cgtcggttcg     2940

ggctgccacg accgggcgac agcgacgctg tttcactggt tatgcggcga atccgaaggg     3000

agaacgtcga tgctggtgag cgtaccaagc aggccctagc gttcgagcgc actgacttcg     3060

accaggttcg tgcactcatg gaaaatagcg aacggggcca ggatatacgt actctggcac     3120

ttctgggggt tgcttataac accctgttac gcgtatccga aattgccagg attcggatta     3180

aagacatctc acgtactgac ggtgggagga tgctaatcca cattagcaga acgaaaacgc     3240

tggtcagcac cgcaggcgta gagaaggcgc ttagcctggg ggtaactaaa ctggtcgagc     3300

gatggatttc cgtctctggt gtggctagtg acccaaacaa ttacctgttt tgccaggtta     3360

gaataaatgg tgtggccgtg ccgtctgcca ccagccggct atcaaccgac gtcctgcgaa     3420

agatttttga ggctgcccac cgcctgatct acggtgccaa ggatggctct ggtcagagat     3480

acctggcctg gtctgggcac agtgcccgtg tcggagccgc gcgggatatg gcccgcgctg     3540

gggtttcaat agcggagatt atgcaggctg gtggctggac caccgtagag agtgtcatga     3600

actatatccg taacctggac agtgagacag gggcaatggt gcgtctgctg gaagatggcg     3660

actagtctag acgtacgcct gcaggcaagc ttggctgttt tggcggatga gagaagattt     3720

tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg     3780

gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta     3840

gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata     3900

aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac     3960

gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc     4020

ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc     4080

atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca     4140

ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa     4200

aaggaagagt atgagatctc tcattacatt taaccaaaat tatcacaata taagaatgag     4260

atctttaaca ttgccaatta ggtcagtggt cccaagtagt cacttagaaa atctgtgtat     4320

gtgaaatact gtttgtgact taaaatgaaa tttattttta ataggtgaac ataatattga     4380

ggagacagaa catacagagc aaaagcgaaa tgtgattcga attattcctc accacaacta     4440

caatgcagct attaataagt acaaccatga cattgccctt ctggaactgg acgaaccctt     4500

agtgctaaac agctacgtta cacctatttg cattgctgac aaggaataca cgaacatctt     4560

cctcaaattt ggatctggct atgtaagtgg ctggggaaga gtcttccaca aagggagatc     4620

agctttagtt cttcagtacc ttagagttcc acttgttgac cgagccacat gtcttcgatc     4680

tacaaagttc accatctata acaacatgtt ctgtgctggc ttccatgaag gaggtagaga     4740

ttcatgtcaa ggagatagtg ggggacccca tgttactgaa gtggaaggga ccagtttctt     4800

aactggaatt attagctggg gtgaagagtg tgcaatgaaa ggcaaatatg gaatatatac     4860

caaggtatcc cggtatgtca actggattaa ggaaaaaaca aagctcactt aatgaaagat     4920

ggatttccaa ggttaattca ttggaattga aaattaacag ggcctctcac taactaatca     4980

ctttcccatc ttttgttaga tttgaatata tacattctaa gatctggtcg aatttgcttt     5040

cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa     5100

gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt     5160

tgtaattcat taagcattct gccgacatgg aagccatcac agacggcatg atgaacctga     5220

atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg     5280

ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag     5340

ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt     5400

tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg     5460

tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg     5520

tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga     5580

gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc     5640

tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga     5700

gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg     5760

gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac     5820

tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg     5880

tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg     5940

acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa     6000

agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc     6060

tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg     6120

taacggcaaa agcaccgccg gacatcagcg ctagcggagt gtatactggc ttactatgtt     6180

ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg     6240

tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac     6300

gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg     6360

aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca     6420

taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa     6480

cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc     6540

tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc     6600

cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac     6660

cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg     6720

aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc     6780

ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct     6840

ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc     6900

tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa     6960

gaagatcatc ttattaatca gataaaatat ttctag                               6996


<210>  39
<211>  343
<212>  PRT
<213>  Bacteriophage P1

<400>  39

Met Ser Asn Leu Leu Thr Val His Gln Asn Leu Pro Ala Leu Pro Val 
1               5                   10                  15      


Asp Ala Thr Ser Asp Glu Val Arg Lys Asn Leu Met Asp Met Phe Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Glu His Thr Trp Lys Met Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu Tyr Leu Gln Ala 
65                  70                  75                  80  


Arg Gly Leu Ala Val Lys Thr Ile Gln Gln His Leu Gly Gln Leu Asn 
                85                  90                  95      


Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Ser Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             


Glu Arg Ala Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ala Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Val Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 


Arg Val Arg Lys Asn Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     


Ser Thr Arg Ala Leu Glu Gly Ile Phe Glu Ala Thr His Arg Leu Ile 
            260                 265                 270         


Tyr Gly Ala Lys Asp Asp Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Ile Pro Glu Ile Met Gln Ala Gly Gly Trp Thr Asn Val Asn Ile 
305                 310                 315                 320 


Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Gly Asp 
            340             


<210>  40
<211>  34
<212>  DNA
<213>  Homo sapiens

<400>  40
tacacagtgt atattgattt ttatcaaatg cctt                                   34


<210>  41
<211>  34
<212>  DNA
<213>  Homo sapiens

<400>  41
tacacaatgt atattgattt ttatcaaatg cctt                                   34


<210>  42
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Intermediate target site HexL

<400>  42
tacacagtgt atattgattt ttatacattg tgta                                   34


<210>  43
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Intermediate target site HexR

<400>  43
aaggcatttg atattgattt ttatcaaatg cctt                                   34


<210>  44
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Intermediate target site HexR1

<400>  44
aagacatttt atattgattt ttataaaatg tctt                                   34


<210>  45
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Intermediate target site HexR2

<400>  45
aacgcattgg atattgattt ttatccaatg cgtt                                   34


<210>  46
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinase Hex-R-#7

<400>  46

Met Ser Asn Leu Gln Thr Leu His Gln Asn Leu Ser Ala Leu Leu Ala 
1               5                   10                  15      


Asp Ala Thr Ser Asp Glu Ala Arg Lys Asn Leu Thr Asp Val Leu Arg 
            20                  25                  30          


Asp Ser Gln Ala Phe Ser Glu His Thr Trp Arg Val Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Glu Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu His Leu Gln Ala 
65                  70                  75                  80  


Arg Gly Leu Thr Val Asn Thr Ile Gln Gln His Leu Cys Gln Leu Asn 
                85                  90                  95      


Met Leu His Arg Arg Ser Gly Leu Pro Pro Pro Gly Asp Ser Asn Ala 
            100                 105                 110         


Ala Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             


Glu Arg Val Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Leu Asp Gln 
    130                 135                 140                 


Val Cys Ala Leu Met Glu Asn Ser Asn Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Val Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     


Val Ala Arg Ile Arg Val Arg Asp Ile Thr Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Arg Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Phe Leu Phe Cys 
225                 230                 235                 240 


Arg Val Gly Arg Asn Gly Val Ala Val Ser Ser Ala Thr Ser Gln Leu 
                245                 250                 255     


Ser Thr Pro Ala Leu Gln Gly Ile Phe Ala Ser Ala His Arg Leu Ile 
            260                 265                 270         


Tyr Gly Ala Arg Asp Asp Pro Gly Gln Arg Tyr Leu Thr Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Pro Ile Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Ile Glu Ser 
305                 310                 315                 320 


Val Met Asn Tyr Leu Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Gly Asp Asp 
            340             


<210>  47
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinase Hex-L-#7

<400>  47

Met Ser Asn Leu Gln Thr Leu His Gln Asn Leu Ser Ala Leu Leu Val 
1               5                   10                  15      


Asp Val Thr Ser Asp Glu Ala Arg Lys Ser Leu Met Asp Met Leu Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Glu His Thr Trp Lys Met Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Glu Ser Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu His Leu Gln Ala 
65                  70                  75                  80  


Arg Gly Leu Ala Val Arg Thr Ile Gln Gln His Leu Cys Gln Leu Asn 
                85                  90                  95      


Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Gly Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             


Glu Arg Ala Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asp Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Val Arg Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Ile Ser Thr Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 


Arg Val Arg Lys His Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     


Ser Thr Asn Ala Leu Gln Arg Ile Phe Glu Ala Ala His Cys Leu Ile 
            260                 265                 270         


Tyr Gly Ala Lys Asp Asp Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Ile Pro Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Val Asn Ser 
305                 310                 315                 320 


Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Glu Gly Asp 
            340             


<210>  48
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinase Hex-R-#30

<400>  48

Met Ser Tyr Leu His Thr Leu His Gln Ser Leu Ser Ala Leu Leu Val 
1               5                   10                  15      


Asn Ala Thr Ser Asp Glu Ala Arg Lys Asn Leu Met Asp Val Phe Arg 
            20                  25                  30          


Asp Cys Gln Ala Phe Ser Glu His Thr Trp Arg Val Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Val Thr Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu His Leu Gln Ala 
65                  70                  75                  80  


Arg Gly Leu Thr Val Asn Thr Ile Gln Gln His Leu Cys Gln Leu Asn 
                85                  90                  95      


Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Gly Asp Ser Asn Ala 
            100                 105                 110         


Val Thr Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             


Glu Arg Thr Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Gly Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Arg Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Val Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Val Arg Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             


Ala Glu Lys Ala Leu Ser Gln Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 


Arg Val Arg Arg Asn Gly Val Ala Ala Pro Ser Ala Thr Gly Gln Leu 
                245                 250                 255     


Ser Thr Pro Ala Leu Gln Gly Ile Phe Val Ser Ala His Arg Leu Val 
            260                 265                 270         


Tyr Gly Ala Arg Asp Ala Ser Gly Gln Arg Tyr Leu Thr Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Thr Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Pro Ile Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Ile Glu Ser 
305                 310                 315                 320 


Val Met Asn Tyr Leu Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Asp Asp 
            340             


<210>  49
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinase Hex-L-#30

<400>  49

Met Ser Asn Leu Gln Thr Leu His Gln Asn Leu Ser Ala Leu Leu Val 
1               5                   10                  15      


Asp Ala Thr Cys Asp Glu Ala Arg Glu Asn Leu Leu Asn Met Phe Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Glu His Thr Trp Lys Met Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Glu Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu His Leu Gln Ala 
65                  70                  75                  80  


Arg Gly Leu Ala Val Lys Thr Ile Gln Gln His Leu Ala Gln Leu Asn 
                85                  90                  95      


Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Gly Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Val Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             


Glu Arg Ala Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Val Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 


Arg Val Ser Lys Tyr Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     


Ser Thr His Ala Leu Gln Arg Ile Phe Glu Ala Ala His Arg Leu Ile 
            260                 265                 270         


Tyr Gly Ala Lys Asp Asp Ser Gly Arg Arg Tyr Leu Ala Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Ile Pro Glu Ile Met Gln Ala Gly Gly Trp Ala Thr Val Asn Ser 
305                 310                 315                 320 


Val Met Asn Tyr Ser Arg Asn Leu Asp Ser Glu Ala Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Gly Asp 
            340             


