                         SEQUENCE LISTING

<110>  Albumedix Ltd
 
<120>  IMPROVED PROTEIN EXPRESSION STRAINS

<130>  13169-WO-PCT

<150>  EP17176932.6
<151>  2017-06-20

<160>  52    

<170>  PatentIn version 3.5

<210>  1
<211>  2037
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  1
atgggactct tagctttggg cacgcctttg cagtggtttg agtctaggac gtacaatgaa       60

cacataaggg atgaaggtat cgagcagttg ttgtatattt tccaagctgc tggtaaaaga      120

gacaatgacc ctcttttttg gggagacgag cttgagtaca tggttgtaga ttttgatgat      180

aaggagagaa attctatgct cgacgtttgc catgacaaga tactcactga gcttaatatg      240

gaggattcgt ccctttgtga ggctaacgat gtgagttttc accctgagta tggccggtat      300

atgttagagg caacaccagc ttctccatat ttgaattacg tgggtagtta cgttgaggtt      360

aacatgcaaa aaagacgtgc cattgcagaa tataagctat ctgaatatgc gagacaagat      420

agtaaaaata acttgcatgt gggctccagg tctgtccctt tgacgctgac tgtcttcccg      480

aggatgggat gccccgactt tattaacatt aaggatccgt ggaatcataa aaatgccgct      540

tccaggtctc tgtttttacc cgatgaagtc attaacagac atgtcaggtt tcctaacttg      600

acagcatcca tcaggaccag gcgtggtgaa aaagtttgca tgaatgttcc catgtataaa      660

gatatagcta ctccagaaac ggatgactcc atctacgatc gagattggtt tttaccagaa      720

gacaaagagg cgaaactggc ttccaaaccg ggtttcattt atatggattc catgggtttt      780

ggcatgggct gttcgtgctt acaagtgacc tttcaggcac ccaatatcaa caaggcacgt      840

tacctgtacg atgcattagt gaattttgca cctataatgc tagccttctc tgccgctgcg      900

cctgctttta aaggttggct agccgaccaa gatgttcgtt ggaatgtgat atctggtgcg      960

gtggacgacc gtactccgaa ggaaagaggt gttgcgccat tactacccaa atacaacaag     1020

aacggatttg gaggcattgc caaagacgta caagataaag tccttgaaat accaaagtca     1080

agatatagtt cggttgatct tttcttgggt gggtcgaaat ttttcaatag gacttataac     1140

gacacaaatg tacctattaa tgaaaaagta ttaggacgac tactagagaa tgataaggcg     1200

ccactggact atgatcttgc taaacatttt gcgcatctct acataagaga tccagtatct     1260

acattcgaag aactgttgaa tcaggacaac aaaacgtctt caaatcactt tgaaaacatc     1320

caaagtacaa attggcagac attacgtttt aaacccccca cacaacaagc aaccccggac     1380

aaaaaggatt ctcctggttg gagagtggaa ttcagaccat ttgaagtgca actattagat     1440

tttgagaacg ctgcgtattc cgtgctcata tacttgattg tcgatagcat tttgaccttt     1500

tccgataata ttaacgcata tattcatatg tccaaagtat gggaaaatat gaagatagcc     1560

catcacagag atgctatcct atttgaaaaa tttcattgga aaaaatcatt tcgcaacgac     1620

accgatgtgg aaactgaaga ttattctata agcgagattt tccataatcc agagaatggt     1680

atatttcctc aatttgttac gccaatccta tgccaaaaag ggtttgtaac caaagattgg     1740

aaagaattaa agcattcttc caaacacgag agactatact attatttaaa gctaatttct     1800

gatagagcaa gcggtgaatt gccaacaaca gcaaaattct ttagaaattt tgtactacaa     1860

catccagatt acaaacatga ttcaaaaatt tcaaagtcga tcaattatga tttgctttct     1920

acgtgtgata gacttaccca tttagacgat tcaaaaggtg aattgacatc ctttttagga     1980

gctgaaattg cagaatatgt aaaaaaaaat aagccttcaa tagaaagcaa atgttaa        2037


<210>  2
<211>  678
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  2

Met Gly Leu Leu Ala Leu Gly Thr Pro Leu Gln Trp Phe Glu Ser Arg 
1               5                   10                  15      


Thr Tyr Asn Glu His Ile Arg Asp Glu Gly Ile Glu Gln Leu Leu Tyr 
            20                  25                  30          


Ile Phe Gln Ala Ala Gly Lys Arg Asp Asn Asp Pro Leu Phe Trp Gly 
        35                  40                  45              


Asp Glu Leu Glu Tyr Met Val Val Asp Phe Asp Asp Lys Glu Arg Asn 
    50                  55                  60                  


Ser Met Leu Asp Val Cys His Asp Lys Ile Leu Thr Glu Leu Asn Met 
65                  70                  75                  80  


Glu Asp Ser Ser Leu Cys Glu Ala Asn Asp Val Ser Phe His Pro Glu 
                85                  90                  95      


Tyr Gly Arg Tyr Met Leu Glu Ala Thr Pro Ala Ser Pro Tyr Leu Asn 
            100                 105                 110         


Tyr Val Gly Ser Tyr Val Glu Val Asn Met Gln Lys Arg Arg Ala Ile 
        115                 120                 125             


Ala Glu Tyr Lys Leu Ser Glu Tyr Ala Arg Gln Asp Ser Lys Asn Asn 
    130                 135                 140                 


Leu His Val Gly Ser Arg Ser Val Pro Leu Thr Leu Thr Val Phe Pro 
145                 150                 155                 160 


Arg Met Gly Cys Pro Asp Phe Ile Asn Ile Lys Asp Pro Trp Asn His 
                165                 170                 175     


Lys Asn Ala Ala Ser Arg Ser Leu Phe Leu Pro Asp Glu Val Ile Asn 
            180                 185                 190         


Arg His Val Arg Phe Pro Asn Leu Thr Ala Ser Ile Arg Thr Arg Arg 
        195                 200                 205             


Gly Glu Lys Val Cys Met Asn Val Pro Met Tyr Lys Asp Ile Ala Thr 
    210                 215                 220                 


Pro Glu Thr Asp Asp Ser Ile Tyr Asp Arg Asp Trp Phe Leu Pro Glu 
225                 230                 235                 240 


Asp Lys Glu Ala Lys Leu Ala Ser Lys Pro Gly Phe Ile Tyr Met Asp 
                245                 250                 255     


Ser Met Gly Phe Gly Met Gly Cys Ser Cys Leu Gln Val Thr Phe Gln 
            260                 265                 270         


Ala Pro Asn Ile Asn Lys Ala Arg Tyr Leu Tyr Asp Ala Leu Val Asn 
        275                 280                 285             


Phe Ala Pro Ile Met Leu Ala Phe Ser Ala Ala Ala Pro Ala Phe Lys 
    290                 295                 300                 


Gly Trp Leu Ala Asp Gln Asp Val Arg Trp Asn Val Ile Ser Gly Ala 
305                 310                 315                 320 


Val Asp Asp Arg Thr Pro Lys Glu Arg Gly Val Ala Pro Leu Leu Pro 
                325                 330                 335     


Lys Tyr Asn Lys Asn Gly Phe Gly Gly Ile Ala Lys Asp Val Gln Asp 
            340                 345                 350         


Lys Val Leu Glu Ile Pro Lys Ser Arg Tyr Ser Ser Val Asp Leu Phe 
        355                 360                 365             


Leu Gly Gly Ser Lys Phe Phe Asn Arg Thr Tyr Asn Asp Thr Asn Val 
    370                 375                 380                 


Pro Ile Asn Glu Lys Val Leu Gly Arg Leu Leu Glu Asn Asp Lys Ala 
385                 390                 395                 400 


Pro Leu Asp Tyr Asp Leu Ala Lys His Phe Ala His Leu Tyr Ile Arg 
                405                 410                 415     


Asp Pro Val Ser Thr Phe Glu Glu Leu Leu Asn Gln Asp Asn Lys Thr 
            420                 425                 430         


Ser Ser Asn His Phe Glu Asn Ile Gln Ser Thr Asn Trp Gln Thr Leu 
        435                 440                 445             


Arg Phe Lys Pro Pro Thr Gln Gln Ala Thr Pro Asp Lys Lys Asp Ser 
    450                 455                 460                 


Pro Gly Trp Arg Val Glu Phe Arg Pro Phe Glu Val Gln Leu Leu Asp 
465                 470                 475                 480 


Phe Glu Asn Ala Ala Tyr Ser Val Leu Ile Tyr Leu Ile Val Asp Ser 
                485                 490                 495     


Ile Leu Thr Phe Ser Asp Asn Ile Asn Ala Tyr Ile His Met Ser Lys 
            500                 505                 510         


Val Trp Glu Asn Met Lys Ile Ala His His Arg Asp Ala Ile Leu Phe 
        515                 520                 525             


Glu Lys Phe His Trp Lys Lys Ser Phe Arg Asn Asp Thr Asp Val Glu 
    530                 535                 540                 


Thr Glu Asp Tyr Ser Ile Ser Glu Ile Phe His Asn Pro Glu Asn Gly 
545                 550                 555                 560 


Ile Phe Pro Gln Phe Val Thr Pro Ile Leu Cys Gln Lys Gly Phe Val 
                565                 570                 575     


Thr Lys Asp Trp Lys Glu Leu Lys His Ser Ser Lys His Glu Arg Leu 
            580                 585                 590         


Tyr Tyr Tyr Leu Lys Leu Ile Ser Asp Arg Ala Ser Gly Glu Leu Pro 
        595                 600                 605             


Thr Thr Ala Lys Phe Phe Arg Asn Phe Val Leu Gln His Pro Asp Tyr 
    610                 615                 620                 


Lys His Asp Ser Lys Ile Ser Lys Ser Ile Asn Tyr Asp Leu Leu Ser 
625                 630                 635                 640 


Thr Cys Asp Arg Leu Thr His Leu Asp Asp Ser Lys Gly Glu Leu Thr 
                645                 650                 655     


Ser Phe Leu Gly Ala Glu Ile Ala Glu Tyr Val Lys Lys Asn Lys Pro 
            660                 665                 670         


Ser Ile Glu Ser Lys Cys 
        675             


<210>  3
<211>  2037
<212>  DNA
<213>  Artificial sequence

<220>
<223>  GSH1 with A373G mutation

<400>  3
atgggactct tagctttggg cacgcctttg cagtggtttg agtctaggac gtacaatgaa       60

cacataaggg atgaaggtat cgagcagttg ttgtatattt tccaagctgc tggtaaaaga      120

gacaatgacc ctcttttttg gggagacgag cttgagtaca tggttgtaga ttttgatgat      180

aaggagagaa attctatgct cgacgtttgc catgacaaga tactcactga gcttaatatg      240

gaggattcgt ccctttgtga ggctaacgat gtgagttttc accctgagta tggccggtat      300

atgttagagg caacaccagc ttctccatat ttgaattacg tgggtagtta cgttgaggtt      360

aacatgcaaa aaggacgtgc cattgcagaa tataagctat ctgaatatgc gagacaagat      420

agtaaaaata acttgcatgt gggctccagg tctgtccctt tgacgctgac tgtcttcccg      480

aggatgggat gccccgactt tattaacatt aaggatccgt ggaatcataa aaatgccgct      540

tccaggtctc tgtttttacc cgatgaagtc attaacagac atgtcaggtt tcctaacttg      600

acagcatcca tcaggaccag gcgtggtgaa aaagtttgca tgaatgttcc catgtataaa      660

gatatagcta ctccagaaac ggatgactcc atctacgatc gagattggtt tttaccagaa      720

gacaaagagg cgaaactggc ttccaaaccg ggtttcattt atatggattc catgggtttt      780

ggcatgggct gttcgtgctt acaagtgacc tttcaggcac ccaatatcaa caaggcacgt      840

tacctgtacg atgcattagt gaattttgca cctataatgc tagccttctc tgccgctgcg      900

cctgctttta aaggttggct agccgaccaa gatgttcgtt ggaatgtgat atctggtgcg      960

gtggacgacc gtactccgaa ggaaagaggt gttgcgccat tactacccaa atacaacaag     1020

aacggatttg gaggcattgc caaagacgta caagataaag tccttgaaat accaaagtca     1080

agatatagtt cggttgatct tttcttgggt gggtcgaaat ttttcaatag gacttataac     1140

gacacaaatg tacctattaa tgaaaaagta ttaggacgac tactagagaa tgataaggcg     1200

ccactggact atgatcttgc taaacatttt gcgcatctct acataagaga tccagtatct     1260

acattcgaag aactgttgaa tcaggacaac aaaacgtctt caaatcactt tgaaaacatc     1320

caaagtacaa attggcagac attacgtttt aaacccccca cacaacaagc aaccccggac     1380

aaaaaggatt ctcctggttg gagagtggaa ttcagaccat ttgaagtgca actattagat     1440

tttgagaacg ctgcgtattc cgtgctcata tacttgattg tcgatagcat tttgaccttt     1500

tccgataata ttaacgcata tattcatatg tccaaagtat gggaaaatat gaagatagcc     1560

catcacagag atgctatcct atttgaaaaa tttcattgga aaaaatcatt tcgcaacgac     1620

accgatgtgg aaactgaaga ttattctata agcgagattt tccataatcc agagaatggt     1680

atatttcctc aatttgttac gccaatccta tgccaaaaag ggtttgtaac caaagattgg     1740

aaagaattaa agcattcttc caaacacgag agactatact attatttaaa gctaatttct     1800

gatagagcaa gcggtgaatt gccaacaaca gcaaaattct ttagaaattt tgtactacaa     1860

catccagatt acaaacatga ttcaaaaatt tcaaagtcga tcaattatga tttgctttct     1920

acgtgtgata gacttaccca tttagacgat tcaaaaggtg aattgacatc ctttttagga     1980

gctgaaattg cagaatatgt aaaaaaaaat aagccttcaa tagaaagcaa atgttaa        2037


<210>  4
<211>  678
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Gsh1 with Arg125Gly mutation

<400>  4

Met Gly Leu Leu Ala Leu Gly Thr Pro Leu Gln Trp Phe Glu Ser Arg 
1               5                   10                  15      


Thr Tyr Asn Glu His Ile Arg Asp Glu Gly Ile Glu Gln Leu Leu Tyr 
            20                  25                  30          


Ile Phe Gln Ala Ala Gly Lys Arg Asp Asn Asp Pro Leu Phe Trp Gly 
        35                  40                  45              


Asp Glu Leu Glu Tyr Met Val Val Asp Phe Asp Asp Lys Glu Arg Asn 
    50                  55                  60                  


Ser Met Leu Asp Val Cys His Asp Lys Ile Leu Thr Glu Leu Asn Met 
65                  70                  75                  80  


Glu Asp Ser Ser Leu Cys Glu Ala Asn Asp Val Ser Phe His Pro Glu 
                85                  90                  95      


Tyr Gly Arg Tyr Met Leu Glu Ala Thr Pro Ala Ser Pro Tyr Leu Asn 
            100                 105                 110         


Tyr Val Gly Ser Tyr Val Glu Val Asn Met Gln Lys Gly Arg Ala Ile 
        115                 120                 125             


Ala Glu Tyr Lys Leu Ser Glu Tyr Ala Arg Gln Asp Ser Lys Asn Asn 
    130                 135                 140                 


Leu His Val Gly Ser Arg Ser Val Pro Leu Thr Leu Thr Val Phe Pro 
145                 150                 155                 160 


Arg Met Gly Cys Pro Asp Phe Ile Asn Ile Lys Asp Pro Trp Asn His 
                165                 170                 175     


Lys Asn Ala Ala Ser Arg Ser Leu Phe Leu Pro Asp Glu Val Ile Asn 
            180                 185                 190         


Arg His Val Arg Phe Pro Asn Leu Thr Ala Ser Ile Arg Thr Arg Arg 
        195                 200                 205             


Gly Glu Lys Val Cys Met Asn Val Pro Met Tyr Lys Asp Ile Ala Thr 
    210                 215                 220                 


Pro Glu Thr Asp Asp Ser Ile Tyr Asp Arg Asp Trp Phe Leu Pro Glu 
225                 230                 235                 240 


Asp Lys Glu Ala Lys Leu Ala Ser Lys Pro Gly Phe Ile Tyr Met Asp 
                245                 250                 255     


Ser Met Gly Phe Gly Met Gly Cys Ser Cys Leu Gln Val Thr Phe Gln 
            260                 265                 270         


Ala Pro Asn Ile Asn Lys Ala Arg Tyr Leu Tyr Asp Ala Leu Val Asn 
        275                 280                 285             


Phe Ala Pro Ile Met Leu Ala Phe Ser Ala Ala Ala Pro Ala Phe Lys 
    290                 295                 300                 


Gly Trp Leu Ala Asp Gln Asp Val Arg Trp Asn Val Ile Ser Gly Ala 
305                 310                 315                 320 


Val Asp Asp Arg Thr Pro Lys Glu Arg Gly Val Ala Pro Leu Leu Pro 
                325                 330                 335     


Lys Tyr Asn Lys Asn Gly Phe Gly Gly Ile Ala Lys Asp Val Gln Asp 
            340                 345                 350         


Lys Val Leu Glu Ile Pro Lys Ser Arg Tyr Ser Ser Val Asp Leu Phe 
        355                 360                 365             


Leu Gly Gly Ser Lys Phe Phe Asn Arg Thr Tyr Asn Asp Thr Asn Val 
    370                 375                 380                 


Pro Ile Asn Glu Lys Val Leu Gly Arg Leu Leu Glu Asn Asp Lys Ala 
385                 390                 395                 400 


Pro Leu Asp Tyr Asp Leu Ala Lys His Phe Ala His Leu Tyr Ile Arg 
                405                 410                 415     


Asp Pro Val Ser Thr Phe Glu Glu Leu Leu Asn Gln Asp Asn Lys Thr 
            420                 425                 430         


Ser Ser Asn His Phe Glu Asn Ile Gln Ser Thr Asn Trp Gln Thr Leu 
        435                 440                 445             


Arg Phe Lys Pro Pro Thr Gln Gln Ala Thr Pro Asp Lys Lys Asp Ser 
    450                 455                 460                 


Pro Gly Trp Arg Val Glu Phe Arg Pro Phe Glu Val Gln Leu Leu Asp 
465                 470                 475                 480 


Phe Glu Asn Ala Ala Tyr Ser Val Leu Ile Tyr Leu Ile Val Asp Ser 
                485                 490                 495     


Ile Leu Thr Phe Ser Asp Asn Ile Asn Ala Tyr Ile His Met Ser Lys 
            500                 505                 510         


Val Trp Glu Asn Met Lys Ile Ala His His Arg Asp Ala Ile Leu Phe 
        515                 520                 525             


Glu Lys Phe His Trp Lys Lys Ser Phe Arg Asn Asp Thr Asp Val Glu 
    530                 535                 540                 


Thr Glu Asp Tyr Ser Ile Ser Glu Ile Phe His Asn Pro Glu Asn Gly 
545                 550                 555                 560 


Ile Phe Pro Gln Phe Val Thr Pro Ile Leu Cys Gln Lys Gly Phe Val 
                565                 570                 575     


Thr Lys Asp Trp Lys Glu Leu Lys His Ser Ser Lys His Glu Arg Leu 
            580                 585                 590         


Tyr Tyr Tyr Leu Lys Leu Ile Ser Asp Arg Ala Ser Gly Glu Leu Pro 
        595                 600                 605             


Thr Thr Ala Lys Phe Phe Arg Asn Phe Val Leu Gln His Pro Asp Tyr 
    610                 615                 620                 


Lys His Asp Ser Lys Ile Ser Lys Ser Ile Asn Tyr Asp Leu Leu Ser 
625                 630                 635                 640 


Thr Cys Asp Arg Leu Thr His Leu Asp Asp Ser Lys Gly Glu Leu Thr 
                645                 650                 655     


Ser Phe Leu Gly Ala Glu Ile Ala Glu Tyr Val Lys Lys Asn Lys Pro 
            660                 665                 670         


Ser Ile Glu Ser Lys Cys 
        675             


<210>  5
<211>  1764
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  5
atgatgaatc cacacgttca agaaaatttg caagcaatcc acaacgcctt aagcaatttt       60

gatacgtcat ttttatcgga ggatgaagaa gattattgcc ctctttgtat tgaaccaatg      120

gatattactg ataaaaattt ttttccttgt ccctgtggtt atcaaatttg tcaattttgc      180

tacaataata tcagacaaaa tccagaatta aatggccgtt gcccagcatg tcgtcgtaaa      240

tatgatgacg agaacgtcag atacgtcaca ttatctccgg aggagttaaa aatggagaga      300

gccaagctcg ctaggaagga gaaagaaaga aagcatagag aaaaagaacg taaagagaat      360

gaatatacga ataggaaaca tttatctggt accagagtta tccaaaagaa tttagtgtac      420

gttgttggca tcaatcctcc tgttccatac gaggaagttg cgcccactct gaaatctgaa      480

aaatattttg gccaatatgg taagataaat aagattgtgg ttaatagaaa aacaccccat      540

tctaacaaca caaccagcga gcattatcac catcattcac caggatatgg cgtttacata      600

accttcggat ccaaggacga tgctgcaaga tgtatagctc aggtagacgg gacgtatatg      660

gatggccgcc tgatcaaagc tgcctacggt actactaaat actgttcttc ttatttaaga      720

ggattgccat gcccaaatcc caactgtatg tttttgcatg aacctggtga agaagctgat      780

tcttttaata aaagagaact ccacaataaa caacaagcgc aacagcaaag tggcggaact      840

gcattcacta gatctggaat acacaacaat atatctacca gtaccgctgg ttcaaatacc      900

aatttactaa gtgaaaattt cacaggcaca ccttcaccgg cggcgatgag ggctcagtta      960

catcatgaca gccatacaaa cgctggaaca ccggtattaa cacctgctcc ggtccctgca     1020

gggtcaaatc cttggggagt tactcaatca gcaacacctg taacctctat caatctctct     1080

aaaaacagca gctccataaa cttgccaaca ttaaatgatt ctctgggcca tcatactacc     1140

cccacaacag agaataccat cacaagtacg acaactacta ccaataccaa tgctacaagt     1200

cactcccatg gtagcaagaa gaagcaatct cttgctgcag aggaatacaa agatccttat     1260

gacgcactag ggaatgctgt tgactttttg gatgcaagac tacattctct atcaaattat     1320

cagaagcgcc ctatatctat caaatccaat attattgacg aagaaactta taaaaagtat     1380

ccgtctttgt tttcttggga caagattgag gcctcaaaga aaagtgacaa tacattagcc     1440

aacaaacttg tggagatcct ggctataaag ccaatagact acactgcttc tgtcgttcaa     1500

ttcttgcaga gtgtcaatgt tggtgtaaat gacaatatta caatcacaga taatacgaaa     1560

actcccaccc aaccaataag actgcaaacc gtctcacagc aaatccaacc accattaaac     1620

gtcagtaccc ctccaccggg tatctttggt ccacaacata aggttcctat tcagcagcaa     1680

caaatgggtg atacaagctc aagaaattcc tctgatttac taaatcaact aatcaacgga     1740

aggaaaatta tcgccggtaa ttaa                                            1764


<210>  6
<211>  587
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  6

Met Met Asn Pro His Val Gln Glu Asn Leu Gln Ala Ile His Asn Ala 
1               5                   10                  15      


Leu Ser Asn Phe Asp Thr Ser Phe Leu Ser Glu Asp Glu Glu Asp Tyr 
            20                  25                  30          


Cys Pro Leu Cys Ile Glu Pro Met Asp Ile Thr Asp Lys Asn Phe Phe 
        35                  40                  45              


Pro Cys Pro Cys Gly Tyr Gln Ile Cys Gln Phe Cys Tyr Asn Asn Ile 
    50                  55                  60                  


Arg Gln Asn Pro Glu Leu Asn Gly Arg Cys Pro Ala Cys Arg Arg Lys 
65                  70                  75                  80  


Tyr Asp Asp Glu Asn Val Arg Tyr Val Thr Leu Ser Pro Glu Glu Leu 
                85                  90                  95      


Lys Met Glu Arg Ala Lys Leu Ala Arg Lys Glu Lys Glu Arg Lys His 
            100                 105                 110         


Arg Glu Lys Glu Arg Lys Glu Asn Glu Tyr Thr Asn Arg Lys His Leu 
        115                 120                 125             


Ser Gly Thr Arg Val Ile Gln Lys Asn Leu Val Tyr Val Val Gly Ile 
    130                 135                 140                 


Asn Pro Pro Val Pro Tyr Glu Glu Val Ala Pro Thr Leu Lys Ser Glu 
145                 150                 155                 160 


Lys Tyr Phe Gly Gln Tyr Gly Lys Ile Asn Lys Ile Val Val Asn Arg 
                165                 170                 175     


Lys Thr Pro His Ser Asn Asn Thr Thr Ser Glu His Tyr His His His 
            180                 185                 190         


Ser Pro Gly Tyr Gly Val Tyr Ile Thr Phe Gly Ser Lys Asp Asp Ala 
        195                 200                 205             


Ala Arg Cys Ile Ala Gln Val Asp Gly Thr Tyr Met Asp Gly Arg Leu 
    210                 215                 220                 


Ile Lys Ala Ala Tyr Gly Thr Thr Lys Tyr Cys Ser Ser Tyr Leu Arg 
225                 230                 235                 240 


Gly Leu Pro Cys Pro Asn Pro Asn Cys Met Phe Leu His Glu Pro Gly 
                245                 250                 255     


Glu Glu Ala Asp Ser Phe Asn Lys Arg Glu Leu His Asn Lys Gln Gln 
            260                 265                 270         


Ala Gln Gln Gln Ser Gly Gly Thr Ala Phe Thr Arg Ser Gly Ile His 
        275                 280                 285             


Asn Asn Ile Ser Thr Ser Thr Ala Gly Ser Asn Thr Asn Leu Leu Ser 
    290                 295                 300                 


Glu Asn Phe Thr Gly Thr Pro Ser Pro Ala Ala Met Arg Ala Gln Leu 
305                 310                 315                 320 


His His Asp Ser His Thr Asn Ala Gly Thr Pro Val Leu Thr Pro Ala 
                325                 330                 335     


Pro Val Pro Ala Gly Ser Asn Pro Trp Gly Val Thr Gln Ser Ala Thr 
            340                 345                 350         


Pro Val Thr Ser Ile Asn Leu Ser Lys Asn Ser Ser Ser Ile Asn Leu 
        355                 360                 365             


Pro Thr Leu Asn Asp Ser Leu Gly His His Thr Thr Pro Thr Thr Glu 
    370                 375                 380                 


Asn Thr Ile Thr Ser Thr Thr Thr Thr Thr Asn Thr Asn Ala Thr Ser 
385                 390                 395                 400 


His Ser His Gly Ser Lys Lys Lys Gln Ser Leu Ala Ala Glu Glu Tyr 
                405                 410                 415     


Lys Asp Pro Tyr Asp Ala Leu Gly Asn Ala Val Asp Phe Leu Asp Ala 
            420                 425                 430         


Arg Leu His Ser Leu Ser Asn Tyr Gln Lys Arg Pro Ile Ser Ile Lys 
        435                 440                 445             


Ser Asn Ile Ile Asp Glu Glu Thr Tyr Lys Lys Tyr Pro Ser Leu Phe 
    450                 455                 460                 


Ser Trp Asp Lys Ile Glu Ala Ser Lys Lys Ser Asp Asn Thr Leu Ala 
465                 470                 475                 480 


Asn Lys Leu Val Glu Ile Leu Ala Ile Lys Pro Ile Asp Tyr Thr Ala 
                485                 490                 495     


Ser Val Val Gln Phe Leu Gln Ser Val Asn Val Gly Val Asn Asp Asn 
            500                 505                 510         


Ile Thr Ile Thr Asp Asn Thr Lys Thr Pro Thr Gln Pro Ile Arg Leu 
        515                 520                 525             


Gln Thr Val Ser Gln Gln Ile Gln Pro Pro Leu Asn Val Ser Thr Pro 
    530                 535                 540                 


Pro Pro Gly Ile Phe Gly Pro Gln His Lys Val Pro Ile Gln Gln Gln 
545                 550                 555                 560 


Gln Met Gly Asp Thr Ser Ser Arg Asn Ser Ser Asp Leu Leu Asn Gln 
                565                 570                 575     


Leu Ile Asn Gly Arg Lys Ile Ile Ala Gly Asn 
            580                 585         


<210>  7
<211>  1764
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NOT4 with T1285A mutation

<400>  7
atgatgaatc cacacgttca agaaaatttg caagcaatcc acaacgcctt aagcaatttt       60

gatacgtcat ttttatcgga ggatgaagaa gattattgcc ctctttgtat tgaaccaatg      120

gatattactg ataaaaattt ttttccttgt ccctgtggtt atcaaatttg tcaattttgc      180

tacaataata tcagacaaaa tccagaatta aatggccgtt gcccagcatg tcgtcgtaaa      240

tatgatgacg agaacgtcag atacgtcaca ttatctccgg aggagttaaa aatggagaga      300

gccaagctcg ctaggaagga gaaagaaaga aagcatagag aaaaagaacg taaagagaat      360

gaatatacga ataggaaaca tttatctggt accagagtta tccaaaagaa tttagtgtac      420

gttgttggca tcaatcctcc tgttccatac gaggaagttg cgcccactct gaaatctgaa      480

aaatattttg gccaatatgg taagataaat aagattgtgg ttaatagaaa aacaccccat      540

tctaacaaca caaccagcga gcattatcac catcattcac caggatatgg cgtttacata      600

accttcggat ccaaggacga tgctgcaaga tgtatagctc aggtagacgg gacgtatatg      660

gatggccgcc tgatcaaagc tgcctacggt actactaaat actgttcttc ttatttaaga      720

ggattgccat gcccaaatcc caactgtatg tttttgcatg aacctggtga agaagctgat      780

tcttttaata aaagagaact ccacaataaa caacaagcgc aacagcaaag tggcggaact      840

gcattcacta gatctggaat acacaacaat atatctacca gtaccgctgg ttcaaatacc      900

aatttactaa gtgaaaattt cacaggcaca ccttcaccgg cggcgatgag ggctcagtta      960

catcatgaca gccatacaaa cgctggaaca ccggtattaa cacctgctcc ggtccctgca     1020

gggtcaaatc cttggggagt tactcaatca gcaacacctg taacctctat caatctctct     1080

aaaaacagca gctccataaa cttgccaaca ttaaatgatt ctctgggcca tcatactacc     1140

cccacaacag agaataccat cacaagtacg acaactacta ccaataccaa tgctacaagt     1200

cactcccatg gtagcaagaa gaagcaatct cttgctgcag aggaatacaa agatccttat     1260

gacgcactag ggaatgctgt tgacattttg gatgcaagac tacattctct atcaaattat     1320

cagaagcgcc ctatatctat caaatccaat attattgacg aagaaactta taaaaagtat     1380

ccgtctttgt tttcttggga caagattgag gcctcaaaga aaagtgacaa tacattagcc     1440

aacaaacttg tggagatcct ggctataaag ccaatagact acactgcttc tgtcgttcaa     1500

ttcttgcaga gtgtcaatgt tggtgtaaat gacaatatta caatcacaga taatacgaaa     1560

actcccaccc aaccaataag actgcaaacc gtctcacagc aaatccaacc accattaaac     1620

gtcagtaccc ctccaccggg tatctttggt ccacaacata aggttcctat tcagcagcaa     1680

caaatgggtg atacaagctc aagaaattcc tctgatttac taaatcaact aatcaacgga     1740

aggaaaatta tcgccggtaa ttaa                                            1764


<210>  8
<211>  587
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Not4 with Phe429Ile mutation

<400>  8

Met Met Asn Pro His Val Gln Glu Asn Leu Gln Ala Ile His Asn Ala 
1               5                   10                  15      


Leu Ser Asn Phe Asp Thr Ser Phe Leu Ser Glu Asp Glu Glu Asp Tyr 
            20                  25                  30          


Cys Pro Leu Cys Ile Glu Pro Met Asp Ile Thr Asp Lys Asn Phe Phe 
        35                  40                  45              


Pro Cys Pro Cys Gly Tyr Gln Ile Cys Gln Phe Cys Tyr Asn Asn Ile 
    50                  55                  60                  


Arg Gln Asn Pro Glu Leu Asn Gly Arg Cys Pro Ala Cys Arg Arg Lys 
65                  70                  75                  80  


Tyr Asp Asp Glu Asn Val Arg Tyr Val Thr Leu Ser Pro Glu Glu Leu 
                85                  90                  95      


Lys Met Glu Arg Ala Lys Leu Ala Arg Lys Glu Lys Glu Arg Lys His 
            100                 105                 110         


Arg Glu Lys Glu Arg Lys Glu Asn Glu Tyr Thr Asn Arg Lys His Leu 
        115                 120                 125             


Ser Gly Thr Arg Val Ile Gln Lys Asn Leu Val Tyr Val Val Gly Ile 
    130                 135                 140                 


Asn Pro Pro Val Pro Tyr Glu Glu Val Ala Pro Thr Leu Lys Ser Glu 
145                 150                 155                 160 


Lys Tyr Phe Gly Gln Tyr Gly Lys Ile Asn Lys Ile Val Val Asn Arg 
                165                 170                 175     


Lys Thr Pro His Ser Asn Asn Thr Thr Ser Glu His Tyr His His His 
            180                 185                 190         


Ser Pro Gly Tyr Gly Val Tyr Ile Thr Phe Gly Ser Lys Asp Asp Ala 
        195                 200                 205             


Ala Arg Cys Ile Ala Gln Val Asp Gly Thr Tyr Met Asp Gly Arg Leu 
    210                 215                 220                 


Ile Lys Ala Ala Tyr Gly Thr Thr Lys Tyr Cys Ser Ser Tyr Leu Arg 
225                 230                 235                 240 


Gly Leu Pro Cys Pro Asn Pro Asn Cys Met Phe Leu His Glu Pro Gly 
                245                 250                 255     


Glu Glu Ala Asp Ser Phe Asn Lys Arg Glu Leu His Asn Lys Gln Gln 
            260                 265                 270         


Ala Gln Gln Gln Ser Gly Gly Thr Ala Phe Thr Arg Ser Gly Ile His 
        275                 280                 285             


Asn Asn Ile Ser Thr Ser Thr Ala Gly Ser Asn Thr Asn Leu Leu Ser 
    290                 295                 300                 


Glu Asn Phe Thr Gly Thr Pro Ser Pro Ala Ala Met Arg Ala Gln Leu 
305                 310                 315                 320 


His His Asp Ser His Thr Asn Ala Gly Thr Pro Val Leu Thr Pro Ala 
                325                 330                 335     


Pro Val Pro Ala Gly Ser Asn Pro Trp Gly Val Thr Gln Ser Ala Thr 
            340                 345                 350         


Pro Val Thr Ser Ile Asn Leu Ser Lys Asn Ser Ser Ser Ile Asn Leu 
        355                 360                 365             


Pro Thr Leu Asn Asp Ser Leu Gly His His Thr Thr Pro Thr Thr Glu 
    370                 375                 380                 


Asn Thr Ile Thr Ser Thr Thr Thr Thr Thr Asn Thr Asn Ala Thr Ser 
385                 390                 395                 400 


His Ser His Gly Ser Lys Lys Lys Gln Ser Leu Ala Ala Glu Glu Tyr 
                405                 410                 415     


Lys Asp Pro Tyr Asp Ala Leu Gly Asn Ala Val Asp Ile Leu Asp Ala 
            420                 425                 430         


Arg Leu His Ser Leu Ser Asn Tyr Gln Lys Arg Pro Ile Ser Ile Lys 
        435                 440                 445             


Ser Asn Ile Ile Asp Glu Glu Thr Tyr Lys Lys Tyr Pro Ser Leu Phe 
    450                 455                 460                 


Ser Trp Asp Lys Ile Glu Ala Ser Lys Lys Ser Asp Asn Thr Leu Ala 
465                 470                 475                 480 


Asn Lys Leu Val Glu Ile Leu Ala Ile Lys Pro Ile Asp Tyr Thr Ala 
                485                 490                 495     


Ser Val Val Gln Phe Leu Gln Ser Val Asn Val Gly Val Asn Asp Asn 
            500                 505                 510         


Ile Thr Ile Thr Asp Asn Thr Lys Thr Pro Thr Gln Pro Ile Arg Leu 
        515                 520                 525             


Gln Thr Val Ser Gln Gln Ile Gln Pro Pro Leu Asn Val Ser Thr Pro 
    530                 535                 540                 


Pro Pro Gly Ile Phe Gly Pro Gln His Lys Val Pro Ile Gln Gln Gln 
545                 550                 555                 560 


Gln Met Gly Asp Thr Ser Ser Arg Asn Ser Ser Asp Leu Leu Asn Gln 
                565                 570                 575     


Leu Ile Asn Gly Arg Lys Ile Ile Ala Gly Asn 
            580                 585         


<210>  9
<211>  1755
<212>  DNA
<213>  Homo sapiens

<400>  9
gatgcacaca agagtgaggt tgctcatcgg tttaaagatt tgggagaaga aaatttcaaa       60

gccttggtgt tgattgcctt tgctcagtat cttcagcagt gtccatttga agatcatgta      120

aaattagtga atgaagtaac tgaatttgca aaaacatgtg ttgctgatga gtcagctgaa      180

aattgtgaca aatcacttca tacccttttt ggagacaaat tatgcacagt tgcaactctt      240

cgtgaaacct atggtgaaat ggctgactgc tgtgcaaaac aagaacctga gagaaatgaa      300

tgcttcttgc aacacaaaga tgacaaccca aacctccccc gattggtgag accagaggtt      360

gatgtgatgt gcactgcttt tcatgacaat gaagagacat ttttgaaaaa atacttatat      420

gaaattgcca gaagacatcc ttacttttat gccccggaac tccttttctt tgctaaaagg      480

tataaagctg cttttacaga atgttgccaa gctgctgata aagctgcctg cctgttgcca      540

aagctcgatg aacttcggga tgaagggaag gcttcgtctg ccaaacagag actcaagtgt      600

gccagtctcc aaaaatttgg agaaagagct ttcaaagcat gggcagtagc tcgcctgagc      660

cagagatttc ccaaagctga gtttgcagaa gtttccaagt tagtgacaga tcttaccaaa      720

gtccacacgg aatgctgcca tggagatctg cttgaatgtg ctgatgacag ggcggacctt      780

gccaagtata tctgtgaaaa tcaagattcg atctccagta aactgaagga atgctgtgaa      840

aaacctctgt tggaaaaatc ccactgcatt gccgaagtgg aaaatgatga gatgcctgct      900

gacttgcctt cattagctgc tgattttgtt gaaagtaagg atgtttgcaa aaactatgct      960

gaggcaaagg atgtcttcct gggcatgttt ttgtatgaat atgcaagaag gcatcctgat     1020

tactctgtcg tgctgctgct gagacttgcc aagacatatg aaaccactct agagaagtgc     1080

tgtgccgctg cagatcctca tgaatgctat gccaaagtgt tcgatgaatt taaacctctt     1140

gtggaagagc ctcagaattt aatcaaacaa aattgtgagc tttttgagca gcttggagag     1200

tacaaattcc agaatgcgct attagttcgt tacaccaaga aagtacccca agtgtcaact     1260

ccaactcttg tagaggtctc aagaaaccta ggaaaagtgg gcagcaaatg ttgtaaacat     1320

cctgaagcaa aaagaatgcc ctgtgcagaa gactatctat ccgtggtcct gaaccagtta     1380

tgtgtgttgc atgagaaaac gccagtaagt gacagagtca ccaaatgctg cacagaatcc     1440

ttggtgaaca ggcgaccatg cttttcagct ctggaagtcg atgaaacata cgttcccaaa     1500

gagtttaatg ctgaaacatt caccttccat gcagatatat gcacactttc tgagaaggag     1560

agacaaatca agaaacaaac tgcacttgtt gagctcgtga aacacaagcc caaggcaaca     1620

aaagagcaac tgaaagctgt tatggatgat ttcgcagctt ttgtagagaa gtgctgcaag     1680

gctgacgata aggagacctg ctttgccgag gagggtaaaa aacttgttgc tgcaagtcaa     1740

gctgccttag gctta                                                      1755


<210>  10
<211>  585
<212>  PRT
<213>  Homo sapiens

<400>  10

Asp Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu 
1               5                   10                  15      


Glu Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu Gln 
            20                  25                  30          


Gln Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr Glu 
        35                  40                  45              


Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys 
    50                  55                  60                  


Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu 
65                  70                  75                  80  


Arg Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro 
                85                  90                  95      


Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu 
            100                 105                 110         


Pro Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His 
        115                 120                 125             


Asp Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala Arg 
    130                 135                 140                 


Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys Arg 
145                 150                 155                 160 


Tyr Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala 
                165                 170                 175     


Cys Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser 
            180                 185                 190         


Ser Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Glu 
        195                 200                 205             


Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe Pro 
    210                 215                 220                 


Lys Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys 
225                 230                 235                 240 


Val His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp 
                245                 250                 255     


Arg Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile Ser 
            260                 265                 270         


Ser Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser His 
        275                 280                 285             


Cys Ile Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro Ser 
    290                 295                 300                 


Leu Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala 
305                 310                 315                 320 


Glu Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg 
                325                 330                 335     


Arg His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys Thr 
            340                 345                 350         


Tyr Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu 
        355                 360                 365             


Cys Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro 
    370                 375                 380                 


Gln Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu 
385                 390                 395                 400 


Tyr Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro 
                405                 410                 415     


Gln Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys 
            420                 425                 430         


Val Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys 
        435                 440                 445             


Ala Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu His 
    450                 455                 460                 


Glu Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser 
465                 470                 475                 480 


Leu Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu Thr 
                485                 490                 495     


Tyr Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp 
            500                 505                 510         


Ile Cys Thr Leu Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr Ala 
        515                 520                 525             


Leu Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln Leu 
    530                 535                 540                 


Lys Ala Val Met Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys Lys 
545                 550                 555                 560 


Ala Asp Asp Lys Glu Thr Cys Phe Ala Glu Glu Gly Lys Lys Leu Val 
                565                 570                 575     


Ala Ala Ser Gln Ala Ala Leu Gly Leu 
            580                 585 


<210>  11
<211>  1827
<212>  DNA
<213>  Homo sapiens

<400>  11
atgaagtggg taagctttat ttcccttctt tttctcttta gctcggctta ttccaggagc       60

ttggataaaa gagatgcaca caagagtgag gttgctcatc ggtttaaaga tttgggagaa      120

gaaaatttca aagccttggt gttgattgcc tttgctcagt atcttcagca gtgtccattt      180

gaagatcatg taaaattagt gaatgaagta actgaatttg caaaaacatg tgttgctgat      240

gagtcagctg aaaattgtga caaatcactt catacccttt ttggagacaa attatgcaca      300

gttgcaactc ttcgtgaaac ctatggtgaa atggctgact gctgtgcaaa acaagaacct      360

gagagaaatg aatgcttctt gcaacacaaa gatgacaacc caaacctccc ccgattggtg      420

agaccagagg ttgatgtgat gtgcactgct tttcatgaca atgaagagac atttttgaaa      480

aaatacttat atgaaattgc cagaagacat ccttactttt atgccccgga actccttttc      540

tttgctaaaa ggtataaagc tgcttttaca gaatgttgcc aagctgctga taaagctgcc      600

tgcctgttgc caaagctcga tgaacttcgg gatgaaggga aggcttcgtc tgccaaacag      660

agactcaagt gtgccagtct ccaaaaattt ggagaaagag ctttcaaagc atgggcagta      720

gctcgcctga gccagagatt tcccaaagct gagtttgcag aagtttccaa gttagtgaca      780

gatcttacca aagtccacac ggaatgctgc catggagatc tgcttgaatg tgctgatgac      840

agggcggacc ttgccaagta tatctgtgaa aatcaagatt cgatctccag taaactgaag      900

gaatgctgtg aaaaacctct gttggaaaaa tcccactgca ttgccgaagt ggaaaatgat      960

gagatgcctg ctgacttgcc ttcattagct gctgattttg ttgaaagtaa ggatgtttgc     1020

aaaaactatg ctgaggcaaa ggatgtcttc ctgggcatgt ttttgtatga atatgcaaga     1080

aggcatcctg attactctgt cgtgctgctg ctgagacttg ccaagacata tgaaaccact     1140

ctagagaagt gctgtgccgc tgcagatcct catgaatgct atgccaaagt gttcgatgaa     1200

tttaaacctc ttgtggaaga gcctcagaat ttaatcaaac aaaattgtga gctttttgag     1260

cagcttggag agtacaaatt ccagaatgcg ctattagttc gttacaccaa gaaagtaccc     1320

caagtgtcaa ctccaactct tgtagaggtc tcaagaaacc taggaaaagt gggcagcaaa     1380

tgttgtaaac atcctgaagc aaaaagaatg ccctgtgcag aagactatct atccgtggtc     1440

ctgaaccagt tatgtgtgtt gcatgagaaa acgccagtaa gtgacagagt caccaaatgc     1500

tgcacagaat ccttggtgaa caggcgacca tgcttttcag ctctggaagt cgatgaaaca     1560

tacgttccca aagagtttaa tgctgaaaca ttcaccttcc atgcagatat atgcacactt     1620

tctgagaagg agagacaaat caagaaacaa actgcacttg ttgagctcgt gaaacacaag     1680

cccaaggcaa caaaagagca actgaaagct gttatggatg atttcgcagc ttttgtagag     1740

aagtgctgca aggctgacga taaggagacc tgctttgccg aggagggtaa aaaacttgtt     1800

gctgcaagtc aagctgcctt aggctta                                         1827


<210>  12
<211>  609
<212>  PRT
<213>  Homo sapiens

<400>  12

Met Lys Trp Val Thr Phe Ile Ser Leu Leu Phe Leu Phe Ser Ser Ala 
1               5                   10                  15      


Tyr Ser Arg Gly Val Phe Arg Arg Asp Ala His Lys Ser Glu Val Ala 
            20                  25                  30          


His Arg Phe Lys Asp Leu Gly Glu Glu Asn Phe Lys Ala Leu Val Leu 
        35                  40                  45              


Ile Ala Phe Ala Gln Tyr Leu Gln Gln Cys Pro Phe Glu Asp His Val 
    50                  55                  60                  


Lys Leu Val Asn Glu Val Thr Glu Phe Ala Lys Thr Cys Val Ala Asp 
65                  70                  75                  80  


Glu Ser Ala Glu Asn Cys Asp Lys Ser Leu His Thr Leu Phe Gly Asp 
                85                  90                  95      


Lys Leu Cys Thr Val Ala Thr Leu Arg Glu Thr Tyr Gly Glu Met Ala 
            100                 105                 110         


Asp Cys Cys Ala Lys Gln Glu Pro Glu Arg Asn Glu Cys Phe Leu Gln 
        115                 120                 125             


His Lys Asp Asp Asn Pro Asn Leu Pro Arg Leu Val Arg Pro Glu Val 
    130                 135                 140                 


Asp Val Met Cys Thr Ala Phe His Asp Asn Glu Glu Thr Phe Leu Lys 
145                 150                 155                 160 


Lys Tyr Leu Tyr Glu Ile Ala Arg Arg His Pro Tyr Phe Tyr Ala Pro 
                165                 170                 175     


Glu Leu Leu Phe Phe Ala Lys Arg Tyr Lys Ala Ala Phe Thr Glu Cys 
            180                 185                 190         


Cys Gln Ala Ala Asp Lys Ala Ala Cys Leu Leu Pro Lys Leu Asp Glu 
        195                 200                 205             


Leu Arg Asp Glu Gly Lys Ala Ser Ser Ala Lys Gln Arg Leu Lys Cys 
    210                 215                 220                 


Ala Ser Leu Gln Lys Phe Gly Glu Arg Ala Phe Lys Ala Trp Ala Val 
225                 230                 235                 240 


Ala Arg Leu Ser Gln Arg Phe Pro Lys Ala Glu Phe Ala Glu Val Ser 
                245                 250                 255     


Lys Leu Val Thr Asp Leu Thr Lys Val His Thr Glu Cys Cys His Gly 
            260                 265                 270         


Asp Leu Leu Glu Cys Ala Asp Asp Arg Ala Asp Leu Ala Lys Tyr Ile 
        275                 280                 285             


Cys Glu Asn Gln Asp Ser Ile Ser Ser Lys Leu Lys Glu Cys Cys Glu 
    290                 295                 300                 


Lys Pro Leu Leu Glu Lys Ser His Cys Ile Ala Glu Val Glu Asn Asp 
305                 310                 315                 320 


Glu Met Pro Ala Asp Leu Pro Ser Leu Ala Ala Asp Phe Val Glu Ser 
                325                 330                 335     


Lys Asp Val Cys Lys Asn Tyr Ala Glu Ala Lys Asp Val Phe Leu Gly 
            340                 345                 350         


Met Phe Leu Tyr Glu Tyr Ala Arg Arg His Pro Asp Tyr Ser Val Val 
        355                 360                 365             


Leu Leu Leu Arg Leu Ala Lys Thr Tyr Glu Thr Thr Leu Glu Lys Cys 
    370                 375                 380                 


Cys Ala Ala Ala Asp Pro His Glu Cys Tyr Ala Lys Val Phe Asp Glu 
385                 390                 395                 400 


Phe Lys Pro Leu Val Glu Glu Pro Gln Asn Leu Ile Lys Gln Asn Cys 
                405                 410                 415     


Glu Leu Phe Glu Gln Leu Gly Glu Tyr Lys Phe Gln Asn Ala Leu Leu 
            420                 425                 430         


Val Arg Tyr Thr Lys Lys Val Pro Gln Val Ser Thr Pro Thr Leu Val 
        435                 440                 445             


Glu Val Ser Arg Asn Leu Gly Lys Val Gly Ser Lys Cys Cys Lys His 
    450                 455                 460                 


Pro Glu Ala Lys Arg Met Pro Cys Ala Glu Asp Tyr Leu Ser Val Val 
465                 470                 475                 480 


Leu Asn Gln Leu Cys Val Leu His Glu Lys Thr Pro Val Ser Asp Arg 
                485                 490                 495     


Val Thr Lys Cys Cys Thr Glu Ser Leu Val Asn Arg Arg Pro Cys Phe 
            500                 505                 510         


Ser Ala Leu Glu Val Asp Glu Thr Tyr Val Pro Lys Glu Phe Asn Ala 
        515                 520                 525             


Glu Thr Phe Thr Phe His Ala Asp Ile Cys Thr Leu Ser Glu Lys Glu 
    530                 535                 540                 


Arg Gln Ile Lys Lys Gln Thr Ala Leu Val Glu Leu Val Lys His Lys 
545                 550                 555                 560 


Pro Lys Ala Thr Lys Glu Gln Leu Lys Ala Val Met Asp Asp Phe Ala 
                565                 570                 575     


Ala Phe Val Glu Lys Cys Cys Lys Ala Asp Asp Lys Glu Thr Cys Phe 
            580                 585                 590         


Ala Glu Glu Gly Lys Lys Leu Val Ala Ala Ser Gln Ala Ala Leu Gly 
        595                 600                 605             


Leu 
    


<210>  13
<211>  2108
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  13

Met Leu Ser Ala Thr Tyr Arg Asp Leu Asn Thr Ala Ser Asn Leu Glu 
1               5                   10                  15      


Thr Ser Lys Glu Lys Gln Ala Ala Gln Ile Val Ile Ala Gln Ile Ser 
            20                  25                  30          


Leu Leu Phe Thr Thr Leu Asn Asn Asp Asn Phe Glu Ser Val Glu Arg 
        35                  40                  45              


Glu Ile Arg His Ile Leu Asp Arg Ser Ser Val Asp Ile Tyr Ile Lys 
    50                  55                  60                  


Val Trp Glu Arg Leu Leu Thr Leu Ser Ser Arg Asp Ile Leu Gln Ala 
65                  70                  75                  80  


Gly Lys Phe Leu Leu Gln Glu Asn Leu Leu His Arg Leu Leu Leu Glu 
                85                  90                  95      


Phe Ala Lys Asp Leu Pro Lys Lys Ser Thr Asp Leu Ile Glu Leu Leu 
            100                 105                 110         


Lys Glu Arg Thr Phe Asn Asn Gln Glu Phe Gln Lys Gln Thr Gly Ile 
        115                 120                 125             


Thr Leu Ser Leu Phe Ile Asp Leu Phe Asp Lys Ser Ala Asn Lys Asp 
    130                 135                 140                 


Ile Ile Glu Ser Leu Asp Arg Ser Ser Gln Ile Asn Asp Phe Lys Thr 
145                 150                 155                 160 


Ile Lys Met Asn His Thr Asn Tyr Leu Arg Asn Phe Phe Leu Gln Thr 
                165                 170                 175     


Thr Pro Glu Thr Leu Glu Ser Asn Leu Arg Asp Leu Leu His Ser Leu 
            180                 185                 190         


Glu Gly Glu Ser Leu Asn Asp Leu Leu Ala Leu Leu Leu Ser Glu Ile 
        195                 200                 205             


Leu Ser Pro Gly Ser Gln Asn Leu Gln Asn Asp Pro Thr Arg Ser Trp 
    210                 215                 220                 


Leu Thr Pro Pro Met Val Leu Asp Ala Thr Asn Arg Gly Asn Val Ile 
225                 230                 235                 240 


Ala Arg Ser Ile Ser Ser Leu Gln Ala Asn Gln Ile Asn Trp Asn Arg 
                245                 250                 255     


Val Phe Asn Leu Met Ser Thr Lys Tyr Phe Leu Ser Ala Pro Leu Met 
            260                 265                 270         


Pro Thr Thr Ala Ser Leu Ser Cys Leu Phe Ala Ala Leu His Asp Gly 
        275                 280                 285             


Pro Val Ile Asp Glu Phe Phe Ser Cys Asp Trp Lys Val Ile Phe Lys 
    290                 295                 300                 


Leu Asp Leu Ala Ile Gln Leu His Lys Trp Ser Val Gln Asn Gly Cys 
305                 310                 315                 320 


Phe Asp Leu Leu Asn Ala Glu Gly Thr Arg Lys Val Ser Glu Thr Ile 
                325                 330                 335     


Pro Asn Thr Lys Gln Ser Leu Leu Tyr Leu Leu Ser Ile Ala Ser Leu 
            340                 345                 350         


Asn Leu Glu Leu Phe Leu Gln Arg Glu Glu Leu Ser Asp Gly Pro Met 
        355                 360                 365             


Leu Ala Tyr Phe Gln Glu Cys Phe Phe Glu Asp Phe Asn Tyr Ala Pro 
    370                 375                 380                 


Glu Tyr Leu Ile Leu Ala Leu Val Lys Glu Met Lys Arg Phe Val Leu 
385                 390                 395                 400 


Leu Ile Glu Asn Arg Thr Val Ile Asp Glu Ile Leu Ile Thr Leu Leu 
                405                 410                 415     


Ile Gln Val His Asn Lys Ser Pro Ser Ser Phe Lys Asp Val Ile Ser 
            420                 425                 430         


Thr Ile Thr Asp Asp Ser Lys Ile Val Asp Ala Ala Lys Ile Ile Ile 
        435                 440                 445             


Asn Ser Asp Asp Ala Pro Ile Ala Asn Phe Leu Lys Ser Leu Leu Asp 
    450                 455                 460                 


Thr Gly Arg Leu Asp Thr Val Ile Asn Lys Leu Pro Phe Asn Glu Ala 
465                 470                 475                 480 


Phe Lys Ile Leu Pro Cys Ala Arg Gln Ile Gly Trp Glu Gly Phe Asp 
                485                 490                 495     


Thr Phe Leu Lys Thr Lys Val Ser Pro Ser Asn Val Asp Val Val Leu 
            500                 505                 510         


Glu Ser Leu Glu Val Gln Thr Lys Met Thr Asp Thr Asn Thr Pro Phe 
        515                 520                 525             


Arg Ser Leu Lys Thr Phe Asp Leu Phe Ala Phe His Ser Leu Ile Glu 
    530                 535                 540                 


Val Leu Asn Lys Cys Pro Leu Asp Val Leu Gln Leu Gln Arg Phe Glu 
545                 550                 555                 560 


Ser Leu Glu Phe Ser Leu Leu Ile Ala Phe Pro Arg Leu Ile Asn Phe 
                565                 570                 575     


Gly Phe Gly His Asp Glu Ala Ile Leu Ala Asn Gly Asp Ile Ala Gly 
            580                 585                 590         


Ile Asn Asn Asp Ile Glu Lys Glu Met Gln Asn Tyr Leu Gln Lys Met 
        595                 600                 605             


Tyr Ser Gly Glu Leu Ala Ile Lys Asp Val Ile Glu Leu Leu Arg Arg 
    610                 615                 620                 


Leu Arg Asp Ser Asp Leu Pro Arg Asp Gln Glu Val Phe Thr Cys Ile 
625                 630                 635                 640 


Thr His Ala Val Ile Ala Glu Ser Thr Phe Phe Gln Asp Tyr Pro Leu 
                645                 650                 655     


Asp Ala Leu Ala Thr Thr Ser Val Leu Phe Gly Ser Met Ile Leu Phe 
            660                 665                 670         


Gln Leu Leu Arg Gly Phe Val Leu Asp Val Ala Phe Arg Ile Ile Met 
        675                 680                 685             


Arg Phe Ala Lys Glu Pro Pro Glu Ser Lys Met Phe Lys Phe Ala Val 
    690                 695                 700                 


Gln Ala Ile Tyr Ala Phe Arg Ile Arg Leu Ala Glu Tyr Pro Gln Tyr 
705                 710                 715                 720 


Cys Lys Asp Leu Leu Arg Asp Val Pro Ala Leu Lys Ser Gln Ala Gln 
                725                 730                 735     


Val Tyr Gln Ser Ile Val Glu Ala Ala Thr Leu Ala Asn Ala Pro Lys 
            740                 745                 750         


Glu Arg Ser Arg Pro Val Gln Glu Met Ile Pro Leu Lys Phe Phe Ala 
        755                 760                 765             


Val Asp Glu Val Ser Cys Gln Ile Asn Gln Glu Gly Ala Pro Lys Asp 
    770                 775                 780                 


Val Val Glu Lys Val Leu Phe Val Leu Asn Asn Val Thr Leu Ala Asn 
785                 790                 795                 800 


Leu Asn Asn Lys Val Asp Glu Leu Lys Lys Ser Leu Thr Pro Asn Tyr 
                805                 810                 815     


Phe Ser Trp Phe Ser Thr Tyr Leu Val Thr Gln Arg Ala Lys Thr Glu 
            820                 825                 830         


Pro Asn Tyr His Asp Leu Tyr Ser Lys Val Ile Val Ala Met Gly Ser 
        835                 840                 845             


Gly Leu Leu His Gln Phe Met Val Asn Val Thr Leu Arg Gln Leu Phe 
    850                 855                 860                 


Val Leu Leu Ser Thr Lys Asp Glu Gln Ala Ile Asp Lys Lys His Leu 
865                 870                 875                 880 


Lys Asn Leu Ala Ser Trp Leu Gly Cys Ile Thr Leu Ala Leu Asn Lys 
                885                 890                 895     


Pro Ile Lys His Lys Asn Ile Ala Phe Arg Glu Met Leu Ile Glu Ala 
            900                 905                 910         


Tyr Lys Glu Asn Arg Leu Glu Ile Val Val Pro Phe Val Thr Lys Ile 
        915                 920                 925             


Leu Gln Arg Ala Ser Glu Ser Lys Ile Phe Lys Pro Pro Asn Pro Trp 
    930                 935                 940                 


Thr Val Gly Ile Leu Lys Leu Leu Ile Glu Leu Asn Glu Lys Ala Asn 
945                 950                 955                 960 


Trp Lys Leu Ser Leu Thr Phe Glu Val Glu Val Leu Leu Lys Ser Phe 
                965                 970                 975     


Asn Leu Thr Thr Lys Ser Leu Lys Pro Ser Asn Phe Ile Asn Thr Pro 
            980                 985                 990         


Glu Val Ile Glu Thr Leu Ser Gly  Ala Leu Gly Ser Ile  Thr Leu Glu 
        995                 1000                 1005             


Gln Gln  Gln Thr Glu Gln Gln  Arg Gln Ile Ile Leu  Met Gln Gln 
    1010                 1015                 1020             


His Gln  Gln Gln Met Leu Ile  Tyr Gln Gln Arg Gln  Gln Gln Gln 
    1025                 1030                 1035             


Gln Gln  Arg Gln Gln Gln Gln  Gln His His Ile Ser  Ala Asn Thr 
    1040                 1045                 1050             


Ile Ala  Asp Gln Gln Ala Ala  Phe Gly Gly Glu Gly  Ser Ile Ser 
    1055                 1060                 1065             


His Asp  Asn Pro Phe Asn Asn  Leu Leu Gly Ser Thr  Ile Phe Val 
    1070                 1075                 1080             


Thr His  Pro Asp Leu Lys Arg  Val Phe Gln Met Ala  Leu Ala Lys 
    1085                 1090                 1095             


Ser Val  Arg Glu Ile Leu Leu  Glu Val Val Glu Lys  Ser Ser Gly 
    1100                 1105                 1110             


Ile Ala  Val Val Thr Thr Thr  Lys Ile Ile Leu Lys  Asp Phe Ala 
    1115                 1120                 1125             


Thr Glu  Val Asp Glu Ser Lys  Leu Lys Thr Ala Ala  Ile Ile Met 
    1130                 1135                 1140             


Val Arg  His Leu Ala Gln Ser  Leu Ala Arg Ala Thr  Ser Ile Glu 
    1145                 1150                 1155             


Pro Leu  Lys Glu Gly Ile Arg  Ser Thr Met Gln Ser  Leu Ala Pro 
    1160                 1165                 1170             


Asn Leu  Met Ser Leu Ser Ser  Ser Pro Ala Glu Glu  Leu Asp Thr 
    1175                 1180                 1185             


Ala Ile  Asn Glu Asn Ile Gly  Ile Ala Leu Val Leu  Ile Glu Lys 
    1190                 1195                 1200             


Ala Ser  Met Asp Lys Ser Thr  Gln Asp Leu Ala Asp  Gln Leu Met 
    1205                 1210                 1215             


Gln Ala  Ile Ala Ile Arg Arg  Tyr His Lys Glu Arg  Arg Ala Asp 
    1220                 1225                 1230             


Gln Pro  Phe Ile Thr Gln Asn  Thr Asn Pro Tyr Ser  Leu Ser Leu 
    1235                 1240                 1245             


Pro Glu  Pro Leu Gly Leu Lys  Asn Thr Gly Val Thr  Pro Gln Gln 
    1250                 1255                 1260             


Phe Arg  Val Tyr Glu Glu Phe  Gly Lys Asn Ile Pro  Asn Leu Asp 
    1265                 1270                 1275             


Val Ile  Pro Phe Ala Gly Leu  Pro Ala His Ala Pro  Pro Met Thr 
    1280                 1285                 1290             


Gln Asn  Val Gly Leu Thr Gln  Pro Gln Gln Gln Gln  Ala Gln Met 
    1295                 1300                 1305             


Pro Thr  Gln Ile Leu Thr Ser  Glu Gln Ile Arg Ala  Gln Gln Gln 
    1310                 1315                 1320             


Gln Gln  Gln Leu Gln Lys Ser  Arg Leu Asn Gln Pro  Ser Gln Ser 
    1325                 1330                 1335             


Ala Gln  Pro Pro Gly Val Asn  Val Pro Asn Pro Gln  Gly Gly Ile 
    1340                 1345                 1350             


Ala Ala  Val Gln Ser Asp Leu  Glu Gln Asn Gln Arg  Val Leu Val 
    1355                 1360                 1365             


His Leu  Met Asp Ile Leu Val  Ser Gln Ile Lys Glu  Asn Ala Thr 
    1370                 1375                 1380             


Lys Asn  Asn Leu Ala Glu Leu  Gly Asp Gln Asn Gln  Ile Lys Thr 
    1385                 1390                 1395             


Ile Ile  Phe Gln Ile Leu Thr  Phe Ile Ala Lys Ser  Ala Gln Lys 
    1400                 1405                 1410             


Asp Gln  Leu Ala Leu Lys Val  Ser Gln Ala Val Val  Asn Ser Leu 
    1415                 1420                 1425             


Phe Ala  Thr Ser Glu Ser Pro  Leu Cys Arg Glu Val  Leu Ser Leu 
    1430                 1435                 1440             


Leu Leu  Glu Lys Leu Cys Ser  Leu Ser Leu Val Ala  Arg Lys Asp 
    1445                 1450                 1455             


Val Val  Trp Trp Leu Val Tyr  Ala Leu Asp Ser Arg  Lys Phe Asn 
    1460                 1465                 1470             


Val Pro  Val Ile Arg Ser Leu  Leu Glu Val Asn Leu  Ile Asp Ala 
    1475                 1480                 1485             


Thr Glu  Leu Asp Asn Val Leu  Val Thr Ala Met Lys  Asn Lys Met 
    1490                 1495                 1500             


Glu Asn  Ser Thr Glu Phe Ala  Met Lys Leu Ile Gln  Asn Thr Val 
    1505                 1510                 1515             


Leu Ser  Asp Asp Pro Ile Leu  Met Arg Met Asp Phe  Ile Lys Thr 
    1520                 1525                 1530             


Leu Glu  His Leu Ala Ser Ser  Glu Asp Glu Asn Val  Lys Lys Phe 
    1535                 1540                 1545             


Ile Lys  Glu Phe Glu Asp Thr  Lys Ile Met Pro Val  Arg Lys Gly 
    1550                 1555                 1560             


Thr Lys  Thr Thr Arg Thr Glu  Lys Leu Tyr Leu Val  Phe Thr Glu 
    1565                 1570                 1575             


Trp Val  Lys Leu Leu Gln Arg  Val Glu Asn Asn Asp  Val Ile Thr 
    1580                 1585                 1590             


Thr Val  Phe Ile Lys Gln Leu  Val Glu Lys Gly Val  Ile Ser Asp 
    1595                 1600                 1605             


Thr Asp  Asn Leu Leu Thr Phe  Val Lys Ser Ser Leu  Glu Leu Ser 
    1610                 1615                 1620             


Val Ser  Ser Phe Lys Glu Ser  Asp Pro Thr Asp Glu  Val Phe Ile 
    1625                 1630                 1635             


Ala Ile  Asp Ala Leu Gly Ser  Leu Ile Ile Lys Leu  Leu Ile Leu 
    1640                 1645                 1650             


Gln Gly  Phe Lys Asp Asp Thr  Arg Arg Asp Tyr Ile  Asn Ala Ile 
    1655                 1660                 1665             


Phe Ser  Val Ile Val Leu Val  Phe Ala Lys Asp His  Ser Gln Glu 
    1670                 1675                 1680             


Gly Thr  Thr Phe Asn Glu Arg  Pro Tyr Phe Arg Leu  Phe Ser Asn 
    1685                 1690                 1695             


Ile Leu  Tyr Glu Trp Ala Thr  Ile Arg Thr His Asn  Phe Val Arg 
    1700                 1705                 1710             


Ile Ser  Asp Ser Ser Thr Arg  Gln Glu Leu Ile Glu  Phe Asp Ser 
    1715                 1720                 1725             


Val Phe  Tyr Asn Thr Phe Ser  Gly Tyr Leu His Ala  Leu Gln Pro 
    1730                 1735                 1740             


Phe Ala  Phe Pro Gly Phe Ser  Phe Ala Trp Val Thr  Leu Leu Ser 
    1745                 1750                 1755             


His Arg  Met Leu Leu Pro Ile  Met Leu Arg Leu Pro  Asn Lys Ile 
    1760                 1765                 1770             


Gly Trp  Glu Lys Leu Met Leu  Leu Ile Ile Asp Leu  Phe Lys Phe 
    1775                 1780                 1785             


Leu Asp  Gln Tyr Thr Ser Lys  His Ala Val Ser Asp  Ala Val Ser 
    1790                 1795                 1800             


Val Val  Tyr Lys Gly Thr Leu  Arg Val Ile Leu Gly  Ile Ser Asn 
    1805                 1810                 1815             


Asp Met  Pro Ser Phe Leu Ile  Glu Asn His Tyr Glu  Leu Met Asn 
    1820                 1825                 1830             


Asn Leu  Pro Pro Thr Tyr Phe  Gln Leu Lys Asn Val  Ile Leu Ser 
    1835                 1840                 1845             


Ala Ile  Pro Lys Asn Met Thr  Val Pro Asn Pro Tyr  Asp Val Asp 
    1850                 1855                 1860             


Leu Asn  Met Glu Asp Ile Pro  Ala Cys Lys Glu Leu  Pro Glu Val 
    1865                 1870                 1875             


Phe Phe  Asp Pro Val Ile Asp  Leu His Ser Leu Lys  Lys Pro Val 
    1880                 1885                 1890             


Asp Asn  Tyr Leu Arg Ile Pro  Ser Asn Ser Leu Leu  Arg Thr Ile 
    1895                 1900                 1905             


Leu Ser  Ala Ile Tyr Lys Asp  Thr Tyr Asp Ile Lys  Lys Gly Val 
    1910                 1915                 1920             


Gly Tyr  Asp Phe Leu Ser Val  Asp Ser Lys Leu Ile  Arg Ala Ile 
    1925                 1930                 1935             


Val Leu  His Val Gly Ile Glu  Ala Gly Ile Glu Tyr  Lys Arg Thr 
    1940                 1945                 1950             


Ser Ser  Asn Ala Val Phe Asn  Thr Lys Ser Ser Tyr  Tyr Thr Leu 
    1955                 1960                 1965             


Leu Phe  Asn Leu Ile Gln Asn  Gly Ser Ile Glu Met  Lys Tyr Gln 
    1970                 1975                 1980             


Ile Ile  Leu Ser Ile Val Glu  Gln Leu Arg Tyr Pro  Asn Ile His 
    1985                 1990                 1995             


Thr Tyr  Trp Phe Ser Phe Val  Leu Met Asn Met Phe  Lys Ser Asp 
    2000                 2005                 2010             


Glu Trp  Asn Asp Gln Lys Leu  Glu Val Gln Glu Ile  Ile Leu Arg 
    2015                 2020                 2025             


Asn Phe  Leu Lys Arg Ile Ile  Val Asn Lys Pro His  Thr Trp Gly 
    2030                 2035                 2040             


Val Ser  Val Phe Phe Thr Gln  Leu Ile Asn Asn Asn  Asp Ile Asn 
    2045                 2050                 2055             


Leu Leu  Asp Leu Pro Phe Val  Gln Ser Val Pro Glu  Ile Lys Leu 
    2060                 2065                 2070             


Ile Leu  Gln Gln Leu Val Lys  Tyr Ser Lys Lys Tyr  Thr Thr Ser 
    2075                 2080                 2085             


Glu Gln  Asp Asp Gln Ser Ala  Thr Ile Asn Arg Arg  Gln Thr Pro 
    2090                 2095                 2100             


Leu Gln  Ser Asn Ala 
    2105             


<210>  14
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GLP-1 fragment

<400>  14

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg Gly 
            20                  25                  30      


<210>  15
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tandem repeat of GLP-1 fragment

<400>  15

His Gly Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg His Gly 
            20                  25                  30          


Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala 
        35                  40                  45              


Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg Gly 
    50                  55                  60      


<210>  16
<211>  645
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Albiglutide (GLP-1 tandem repeat fused to the N-terminus of human
       serum albumin)

<400>  16

His Gly Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg His Gly 
            20                  25                  30          


Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala 
        35                  40                  45              


Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg Asp Ala His Lys 
    50                  55                  60                  


Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu Glu Asn Phe Lys 
65                  70                  75                  80  


Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu Gln Gln Cys Pro Phe 
                85                  90                  95      


Glu Asp His Val Lys Leu Val Asn Glu Val Thr Glu Phe Ala Lys Thr 
            100                 105                 110         


Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys Ser Leu His Thr 
        115                 120                 125             


Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu Arg Glu Thr Tyr 
    130                 135                 140                 


Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro Glu Arg Asn Glu 
145                 150                 155                 160 


Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu Pro Arg Leu Val 
                165                 170                 175     


Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His Asp Asn Glu Glu 
            180                 185                 190         


Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala Arg Arg His Pro Tyr 
        195                 200                 205             


Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys Arg Tyr Lys Ala Ala 
    210                 215                 220                 


Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala Cys Leu Leu Pro 
225                 230                 235                 240 


Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser Ser Ala Lys Gln 
                245                 250                 255     


Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Glu Arg Ala Phe Lys 
            260                 265                 270         


Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe Pro Lys Ala Glu Phe 
        275                 280                 285             


Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys Val His Thr Glu 
    290                 295                 300                 


Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp Arg Ala Asp Leu 
305                 310                 315                 320 


Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile Ser Ser Lys Leu Lys 
                325                 330                 335     


Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser His Cys Ile Ala Glu 
            340                 345                 350         


Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro Ser Leu Ala Ala Asp 
        355                 360                 365             


Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala Glu Ala Lys Asp 
    370                 375                 380                 


Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg Arg His Pro Asp 
385                 390                 395                 400 


Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys Thr Tyr Glu Thr Thr 
                405                 410                 415     


Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu Cys Tyr Ala Lys 
            420                 425                 430         


Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro Gln Asn Leu Ile 
        435                 440                 445             


Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu Tyr Lys Phe Gln 
    450                 455                 460                 


Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro Gln Val Ser Thr 
465                 470                 475                 480 


Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys Val Gly Ser Lys 
                485                 490                 495     


Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys Ala Glu Asp Tyr 
            500                 505                 510         


Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu His Glu Lys Thr Pro 
        515                 520                 525             


Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser Leu Val Asn Arg 
    530                 535                 540                 


Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu Thr Tyr Val Pro Lys 
545                 550                 555                 560 


Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp Ile Cys Thr Leu 
                565                 570                 575     


Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr Ala Leu Val Glu Leu 
            580                 585                 590         


Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln Leu Lys Ala Val Met 
        595                 600                 605             


Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys Lys Ala Asp Asp Lys 
    610                 615                 620                 


Glu Thr Cys Phe Ala Glu Glu Gly Lys Lys Leu Val Ala Ala Ser Gln 
625                 630                 635                 640 


Ala Ala Leu Gly Leu 
                645 


<210>  17
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KEX leader sequence

<400>  17

Met Lys Trp Val Ser Phe Ile Ser Leu Leu Phe Leu Phe Ser Ser Ala 
1               5                   10                  15      


Tyr Ser Arg Ser Leu Asp Lys Arg 
            20                  


<210>  18
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified KEX leader sequence

<400>  18

Met Lys Trp Val Ser Phe Ile Ser Leu Leu Phe Leu Phe Ser Ser Ala 
1               5                   10                  15      


Tyr Ser Gly Ser Leu Asp Lys Arg 
            20                  


<210>  19
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mating factor alpha - Human serum albumin fusion leader sequence

<400>  19

Met Lys Trp Val Ser Phe Ile Ser Leu Leu Phe Leu Phe Ser Ser Ala 
1               5                   10                  15      


Tyr Ser Arg Ser Leu Asp Lys Arg Asp Ala His 
            20                  25          


<210>  20
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pentapeptide motif


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa = Phe, Trp or Tyr

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa = Ile, Leu, Val, Ala or Met

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa = Leu, Val, Ala or Met

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa = Ser or Thr

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa = Ile, Val, Ala or Met

<400>  20

Xaa Xaa Xaa Xaa Xaa 
1               5   


<210>  21
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader Sequence


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa = Phe, Trp or Tyr

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa = Ile, Leu, Val, Ala or Met

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa = Leu, Val, Ala or Met

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa = Ser or Thr

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa = Ile, Val, Ala or Met

<400>  21

Met Lys Trp Val Xaa Xaa Xaa Xaa Xaa Leu Phe Leu Phe Ser Ser Ala 
1               5                   10                  15      


Tyr Ser Arg 
            


<210>  22
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader Sequence


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa = Phe, Trp or Tyr

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa = Ile, Leu, Val, Ala or Met

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa = Leu, Val, Ala or Met

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa = Ser or Thr

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa = Ile, Val, Ala or Met

<220>
<221>  PROPEP
<222>  (20)..(24)

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  Xaa = Gly or Ser

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa = Val or Leu

<220>
<221>  MISC_FEATURE
<222>  (22)..(22)
<223>  Xaa = Phe or Asp

<220>
<221>  MISC_FEATURE
<222>  (23)..(23)
<223>  Xaa = Arg or Lys

<220>
<221>  MISC_FEATURE
<222>  (24)..(24)
<223>  Xaa = Arg or Lys

<400>  22

Met Lys Trp Val Xaa Xaa Xaa Xaa Xaa Leu Phe Leu Phe Ser Ser Ala 
1               5                   10                  15      


Tyr Ser Arg Xaa Xaa Xaa Xaa Xaa 
            20                  


<210>  23
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader sequence


<220>
<221>  SIGNAL
<222>  (1)..(19)
<223>  Pre sequence

<220>
<221>  PROPEP
<222>  (20)..(24)

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  Xaa = Gly or Ser

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa = Val or Leu

<220>
<221>  MISC_FEATURE
<222>  (22)..(22)
<223>  Xaa = Phe or Asp

<220>
<221>  MISC_FEATURE
<222>  (23)..(23)
<223>  Xaa = Arg or Lys

<220>
<221>  MISC_FEATURE
<222>  (24)..(24)
<223>  Xaa = Arg or Lys

<400>  23

Met Lys Trp Val Phe Ile Val Ser Ile Leu Phe Leu Phe Ser Ser Ala 
1               5                   10                  15      


Tyr Ser Arg Xaa Xaa Xaa Xaa Xaa 
            20                  


<210>  24
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader sequence

<400>  24

Met Lys Trp Val Phe Ile Val Ser Ile Leu Phe Leu Phe Ser Ser Ala 
1               5                   10                  15      


Tyr Ser Arg Ser Leu Asp Lys Arg 
            20                  


<210>  25
<211>  522
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  25

Met Lys Phe Ser Ala Gly Ala Val Leu Ser Trp Ser Ser Leu Leu Leu 
1               5                   10                  15      


Ala Ser Ser Val Phe Ala Gln Gln Glu Ala Val Ala Pro Glu Asp Ser 
            20                  25                  30          


Ala Val Val Lys Leu Ala Thr Asp Ser Phe Asn Glu Tyr Ile Gln Ser 
        35                  40                  45              


His Asp Leu Val Leu Ala Glu Phe Phe Ala Pro Trp Cys Gly His Cys 
    50                  55                  60                  


Lys Asn Met Ala Pro Glu Tyr Val Lys Ala Ala Glu Thr Leu Val Glu 
65                  70                  75                  80  


Lys Asn Ile Thr Leu Ala Gln Ile Asp Cys Thr Glu Asn Gln Asp Leu 
                85                  90                  95      


Cys Met Glu His Asn Ile Pro Gly Phe Pro Ser Leu Lys Ile Phe Lys 
            100                 105                 110         


Asn Ser Asp Val Asn Asn Ser Ile Asp Tyr Glu Gly Pro Arg Thr Ala 
        115                 120                 125             


Glu Ala Ile Val Gln Phe Met Ile Lys Gln Ser Gln Pro Ala Val Ala 
    130                 135                 140                 


Val Val Ala Asp Leu Pro Ala Tyr Leu Ala Asn Glu Thr Phe Val Thr 
145                 150                 155                 160 


Pro Val Ile Val Gln Ser Gly Lys Ile Asp Ala Asp Phe Asn Ala Thr 
                165                 170                 175     


Phe Tyr Ser Met Ala Asn Lys His Phe Asn Asp Tyr Asp Phe Val Ser 
            180                 185                 190         


Ala Glu Asn Ala Asp Asp Asp Phe Lys Leu Ser Ile Tyr Leu Pro Ser 
        195                 200                 205             


Ala Met Asp Glu Pro Val Val Tyr Asn Gly Lys Lys Ala Asp Ile Ala 
    210                 215                 220                 


Asp Ala Asp Val Phe Glu Lys Trp Leu Gln Val Glu Ala Leu Pro Tyr 
225                 230                 235                 240 


Phe Gly Glu Ile Asp Gly Ser Val Phe Ala Gln Tyr Val Glu Ser Gly 
                245                 250                 255     


Leu Pro Leu Gly Tyr Leu Phe Tyr Asn Asp Glu Glu Glu Leu Glu Glu 
            260                 265                 270         


Tyr Lys Pro Leu Phe Thr Glu Leu Ala Lys Lys Asn Arg Gly Leu Met 
        275                 280                 285             


Asn Phe Val Ser Ile Asp Ala Arg Lys Phe Gly Arg His Ala Gly Asn 
    290                 295                 300                 


Leu Asn Met Lys Glu Gln Phe Pro Leu Phe Ala Ile His Asp Met Thr 
305                 310                 315                 320 


Glu Asp Leu Lys Tyr Gly Leu Pro Gln Leu Ser Glu Glu Ala Phe Asp 
                325                 330                 335     


Glu Leu Ser Asp Lys Ile Val Leu Glu Ser Lys Ala Ile Glu Ser Leu 
            340                 345                 350         


Val Lys Asp Phe Leu Lys Gly Asp Ala Ser Pro Ile Val Lys Ser Gln 
        355                 360                 365             


Glu Ile Phe Glu Asn Gln Asp Ser Ser Val Phe Gln Leu Val Gly Lys 
    370                 375                 380                 


Asn His Asp Glu Ile Val Asn Asp Pro Lys Lys Asp Val Leu Val Leu 
385                 390                 395                 400 


Tyr Tyr Ala Pro Trp Cys Gly His Cys Lys Arg Leu Ala Pro Thr Tyr 
                405                 410                 415     


Gln Glu Leu Ala Asp Thr Tyr Ala Asn Ala Thr Ser Asp Val Leu Ile 
            420                 425                 430         


Ala Lys Leu Asp His Thr Glu Asn Asp Val Arg Gly Val Val Ile Glu 
        435                 440                 445             


Gly Tyr Pro Thr Ile Val Leu Tyr Pro Gly Gly Lys Lys Ser Glu Ser 
    450                 455                 460                 


Val Val Tyr Gln Gly Ser Arg Ser Leu Asp Ser Leu Phe Asp Phe Ile 
465                 470                 475                 480 


Lys Glu Asn Gly His Phe Asp Val Asp Gly Lys Ala Leu Tyr Glu Glu 
                485                 490                 495     


Ala Gln Glu Lys Ala Ala Glu Glu Ala Asp Ala Asp Ala Glu Leu Ala 
            500                 505                 510         


Asp Glu Glu Asp Ala Ile His Asp Glu Leu 
        515                 520         


<210>  26
<211>  563
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  26

Met Arg Leu Arg Thr Ala Ile Ala Thr Leu Cys Leu Thr Ala Phe Thr 
1               5                   10                  15      


Ser Ala Thr Ser Asn Asn Ser Tyr Ile Ala Thr Asp Gln Thr Gln Asn 
            20                  25                  30          


Ala Phe Asn Asp Thr His Phe Cys Lys Val Asp Arg Asn Asp His Val 
        35                  40                  45              


Ser Pro Ser Cys Asn Val Thr Phe Asn Glu Leu Asn Ala Ile Asn Glu 
    50                  55                  60                  


Asn Ile Arg Asp Asp Leu Ser Ala Leu Leu Lys Ser Asp Phe Phe Lys 
65                  70                  75                  80  


Tyr Phe Arg Leu Asp Leu Tyr Lys Gln Cys Ser Phe Trp Asp Ala Asn 
                85                  90                  95      


Asp Gly Leu Cys Leu Asn Arg Ala Cys Ser Val Asp Val Val Glu Asp 
            100                 105                 110         


Trp Asp Thr Leu Pro Glu Tyr Trp Gln Pro Glu Ile Leu Gly Ser Phe 
        115                 120                 125             


Asn Asn Asp Thr Met Lys Glu Ala Asp Asp Ser Asp Asp Glu Cys Lys 
    130                 135                 140                 


Phe Leu Asp Gln Leu Cys Gln Thr Ser Lys Lys Pro Val Asp Ile Glu 
145                 150                 155                 160 


Asp Thr Ile Asn Tyr Cys Asp Val Asn Asp Phe Asn Gly Lys Asn Ala 
                165                 170                 175     


Val Leu Ile Asp Leu Thr Ala Asn Pro Glu Arg Phe Thr Gly Tyr Gly 
            180                 185                 190         


Gly Lys Gln Ala Gly Gln Ile Trp Ser Thr Ile Tyr Gln Asp Asn Cys 
        195                 200                 205             


Phe Thr Ile Gly Glu Thr Gly Glu Ser Leu Ala Lys Asp Ala Phe Tyr 
    210                 215                 220                 


Arg Leu Val Ser Gly Phe His Ala Ser Ile Gly Thr His Leu Ser Lys 
225                 230                 235                 240 


Glu Tyr Leu Asn Thr Lys Thr Gly Lys Trp Glu Pro Asn Leu Asp Leu 
                245                 250                 255     


Phe Met Ala Arg Ile Gly Asn Phe Pro Asp Arg Val Thr Asn Met Tyr 
            260                 265                 270         


Phe Asn Tyr Ala Val Val Ala Lys Ala Leu Trp Lys Ile Gln Pro Tyr 
        275                 280                 285             


Leu Pro Glu Phe Ser Phe Cys Asp Leu Val Asn Lys Glu Ile Lys Asn 
    290                 295                 300                 


Lys Met Asp Asn Val Ile Ser Gln Leu Asp Thr Lys Ile Phe Asn Glu 
305                 310                 315                 320 


Asp Leu Val Phe Ala Asn Asp Leu Ser Leu Thr Leu Lys Asp Glu Phe 
                325                 330                 335     


Arg Ser Arg Phe Lys Asn Val Thr Lys Ile Met Asp Cys Val Gln Cys 
            340                 345                 350         


Asp Arg Cys Arg Leu Trp Gly Lys Ile Gln Thr Thr Gly Tyr Ala Thr 
        355                 360                 365             


Ala Leu Lys Ile Leu Phe Glu Ile Asn Asp Ala Asp Glu Phe Thr Lys 
    370                 375                 380                 


Gln His Ile Val Gly Lys Leu Thr Lys Tyr Glu Leu Ile Ala Leu Leu 
385                 390                 395                 400 


Gln Thr Phe Gly Arg Leu Ser Glu Ser Ile Glu Ser Val Asn Met Phe 
                405                 410                 415     


Glu Lys Met Tyr Gly Lys Arg Leu Asn Gly Ser Glu Asn Arg Leu Ser 
            420                 425                 430         


Ser Phe Phe Gln Asn Asn Phe Phe Asn Ile Leu Lys Glu Ala Gly Lys 
        435                 440                 445             


Ser Ile Arg Tyr Thr Ile Glu Asn Ile Asn Ser Thr Lys Glu Gly Lys 
    450                 455                 460                 


Lys Lys Thr Asn Asn Ser Gln Ser His Val Phe Asp Asp Leu Lys Met 
465                 470                 475                 480 


Pro Lys Ala Glu Ile Val Pro Arg Pro Ser Asn Gly Thr Val Asn Lys 
                485                 490                 495     


Trp Lys Lys Ala Trp Asn Thr Glu Val Asn Asn Val Leu Glu Ala Phe 
            500                 505                 510         


Arg Phe Ile Tyr Arg Ser Tyr Leu Asp Leu Pro Arg Asn Ile Trp Glu 
        515                 520                 525             


Leu Ser Leu Met Lys Val Tyr Lys Phe Trp Asn Lys Phe Ile Gly Val 
    530                 535                 540                 


Ala Asp Tyr Val Ser Glu Glu Thr Arg Glu Pro Ile Ser Tyr Lys Leu 
545                 550                 555                 560 


Asp Ile Gln 
            


<210>  27
<211>  2445
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  27
atgaaagtga ggaaatatat tactttatgc ttttggtggg ccttttcaac atccgctctt       60

gtatcatcac aacaaattcc attgaaggac catacgtcac gacagtattt tgctgtagaa      120

agcaatgaaa cattatcccg cttggaggaa atgcatccaa attggaaata tgaacatgat      180

gttcgagggc taccaaacca ttatgttttt tcaaaagagt tgctaaaatt gggcaaaaga      240

tcatcattag aagagttaca gggggataac aacgaccaca tattatctgt ccatgattta      300

ttcccgcgta acgacctatt taagagacta ccggtgcctg ctccaccaat ggactcaagc      360

ttgttaccgg taaaagaagc tgaggataaa ctcagcataa atgatccgct ttttgagagg      420

cagtggcact tggtcaatcc aagttttcct ggcagtgata taaatgttct tgatctgtgg      480

tacaataata ttacaggcgc aggggtcgtg gctgccattg ttgatgatgg ccttgactac      540

gaaaatgaag acttgaagga taatttttgc gctgaaggtt cttgggattt caacgacaat      600

accaatttac ctaaaccaag attatctgat gactaccatg gtacgagatg tgcaggtgaa      660

atagctgcca aaaaaggtaa caatttttgc ggtgtcgggg taggttacaa cgctaaaatc      720

tcaggcataa gaatcttatc cggtgatatc actacggaag atgaagctgc gtccttgatt      780

tatggtctag acgtaaacga tatatattca tgctcatggg gtcccgctga tgacggaaga      840

catttacaag gccctagtga cctggtgaaa aaggctttag taaaaggtgt tactgaggga      900

agagattcca aaggagcgat ttacgttttt gccagtggaa atggtggaac tcgtggtgat      960

aattgcaatt acgacggcta tactaattcc atatattcta ttactattgg ggctattgat     1020

cacaaagatc tacatcctcc ttattccgaa ggttgttccg ccgtcatggc agtcacgtat     1080

tcttcaggtt caggcgaata tattcattcg agtgatatca acggcagatg cagtaatagc     1140

cacggtggaa cgtctgcggc tgctccatta gctgccggtg tttacacttt gttactagaa     1200

gccaacccaa acctaacttg gagagacgta cagtatttat caatcttgtc tgcggtaggg     1260

ttagaaaaga acgctgacgg agattggaga gatagcgcca tggggaagaa atactctcat     1320

cgctatggct ttggtaaaat cgatgcccat aagttaattg aaatgtccaa gacctgggag     1380

aatgttaacg cacaaacctg gttttacctg ccaacattgt atgtttccca gtccacaaac     1440

tccacggaag agacattaga atccgtcata accatatcag aaaaaagtct tcaagatgct     1500

aacttcaaga gaattgagca cgtcacggta actgtagata ttgatacaga aattagggga     1560

actacgactg tcgatttaat atcaccagcg gggataattt caaaccttgg cgttgtaaga     1620

ccaagagatg tttcatcaga gggattcaaa gactggacat tcatgtctgt agcacattgg     1680

ggtgagaacg gcgtaggtga ttggaaaatc aaggttaaga caacagaaaa tggacacagg     1740

attgacttcc acagttggag gctgaagctc tttggggaat ccattgattc atctaaaaca     1800

gaaactttcg tctttggaaa cgataaagag gaggttgaac cagctgctac agaaagtacc     1860

gtatcacaat attctgccag ttcaacttct atttccatca gcgctacttc tacatcttct     1920

atctcaattg gtgtggaaac gtcggccatt ccccaaacga ctactgcgag taccgatcct     1980

gattctgatc caaacactcc taaaaaactt tcctctccta ggcaagccat gcattatttt     2040

ttaacaatat ttttgattgg cgccacattt ttggtgttat acttcatgtt ttttatgaaa     2100

tcaaggagaa ggatcagaag gtcaagagcg gaaacgtatg aattcgatat cattgataca     2160

gactctgagt acgattctac tttggacaat ggaacttccg gaattactga gcccgaagag     2220

gttgaggact tcgattttga tttgtccgat gaagaccatc ttgcaagttt gtcttcatca     2280

gaaaacggtg atgctgaaca tacaattgat agtgtactaa caaacgaaaa tccatttagt     2340

gaccctataa agcaaaagtt cccaaatgac gccaacgcag aatctgcttc caataaatta     2400

caagaattac agcctgatgt tcctccatct tccggacgat cgtga                     2445


<210>  28
<211>  814
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  28

Met Lys Val Arg Lys Tyr Ile Thr Leu Cys Phe Trp Trp Ala Phe Ser 
1               5                   10                  15      


Thr Ser Ala Leu Val Ser Ser Gln Gln Ile Pro Leu Lys Asp His Thr 
            20                  25                  30          


Ser Arg Gln Tyr Phe Ala Val Glu Ser Asn Glu Thr Leu Ser Arg Leu 
        35                  40                  45              


Glu Glu Met His Pro Asn Trp Lys Tyr Glu His Asp Val Arg Gly Leu 
    50                  55                  60                  


Pro Asn His Tyr Val Phe Ser Lys Glu Leu Leu Lys Leu Gly Lys Arg 
65                  70                  75                  80  


Ser Ser Leu Glu Glu Leu Gln Gly Asp Asn Asn Asp His Ile Leu Ser 
                85                  90                  95      


Val His Asp Leu Phe Pro Arg Asn Asp Leu Phe Lys Arg Leu Pro Val 
            100                 105                 110         


Pro Ala Pro Pro Met Asp Ser Ser Leu Leu Pro Val Lys Glu Ala Glu 
        115                 120                 125             


Asp Lys Leu Ser Ile Asn Asp Pro Leu Phe Glu Arg Gln Trp His Leu 
    130                 135                 140                 


Val Asn Pro Ser Phe Pro Gly Ser Asp Ile Asn Val Leu Asp Leu Trp 
145                 150                 155                 160 


Tyr Asn Asn Ile Thr Gly Ala Gly Val Val Ala Ala Ile Val Asp Asp 
                165                 170                 175     


Gly Leu Asp Tyr Glu Asn Glu Asp Leu Lys Asp Asn Phe Cys Ala Glu 
            180                 185                 190         


Gly Ser Trp Asp Phe Asn Asp Asn Thr Asn Leu Pro Lys Pro Arg Leu 
        195                 200                 205             


Ser Asp Asp Tyr His Gly Thr Arg Cys Ala Gly Glu Ile Ala Ala Lys 
    210                 215                 220                 


Lys Gly Asn Asn Phe Cys Gly Val Gly Val Gly Tyr Asn Ala Lys Ile 
225                 230                 235                 240 


Ser Gly Ile Arg Ile Leu Ser Gly Asp Ile Thr Thr Glu Asp Glu Ala 
                245                 250                 255     


Ala Ser Leu Ile Tyr Gly Leu Asp Val Asn Asp Ile Tyr Ser Cys Ser 
            260                 265                 270         


Trp Gly Pro Ala Asp Asp Gly Arg His Leu Gln Gly Pro Ser Asp Leu 
        275                 280                 285             


Val Lys Lys Ala Leu Val Lys Gly Val Thr Glu Gly Arg Asp Ser Lys 
    290                 295                 300                 


Gly Ala Ile Tyr Val Phe Ala Ser Gly Asn Gly Gly Thr Arg Gly Asp 
305                 310                 315                 320 


Asn Cys Asn Tyr Asp Gly Tyr Thr Asn Ser Ile Tyr Ser Ile Thr Ile 
                325                 330                 335     


Gly Ala Ile Asp His Lys Asp Leu His Pro Pro Tyr Ser Glu Gly Cys 
            340                 345                 350         


Ser Ala Val Met Ala Val Thr Tyr Ser Ser Gly Ser Gly Glu Tyr Ile 
        355                 360                 365             


His Ser Ser Asp Ile Asn Gly Arg Cys Ser Asn Ser His Gly Gly Thr 
    370                 375                 380                 


Ser Ala Ala Ala Pro Leu Ala Ala Gly Val Tyr Thr Leu Leu Leu Glu 
385                 390                 395                 400 


Ala Asn Pro Asn Leu Thr Trp Arg Asp Val Gln Tyr Leu Ser Ile Leu 
                405                 410                 415     


Ser Ala Val Gly Leu Glu Lys Asn Ala Asp Gly Asp Trp Arg Asp Ser 
            420                 425                 430         


Ala Met Gly Lys Lys Tyr Ser His Arg Tyr Gly Phe Gly Lys Ile Asp 
        435                 440                 445             


Ala His Lys Leu Ile Glu Met Ser Lys Thr Trp Glu Asn Val Asn Ala 
    450                 455                 460                 


Gln Thr Trp Phe Tyr Leu Pro Thr Leu Tyr Val Ser Gln Ser Thr Asn 
465                 470                 475                 480 


Ser Thr Glu Glu Thr Leu Glu Ser Val Ile Thr Ile Ser Glu Lys Ser 
                485                 490                 495     


Leu Gln Asp Ala Asn Phe Lys Arg Ile Glu His Val Thr Val Thr Val 
            500                 505                 510         


Asp Ile Asp Thr Glu Ile Arg Gly Thr Thr Thr Val Asp Leu Ile Ser 
        515                 520                 525             


Pro Ala Gly Ile Ile Ser Asn Leu Gly Val Val Arg Pro Arg Asp Val 
    530                 535                 540                 


Ser Ser Glu Gly Phe Lys Asp Trp Thr Phe Met Ser Val Ala His Trp 
545                 550                 555                 560 


Gly Glu Asn Gly Val Gly Asp Trp Lys Ile Lys Val Lys Thr Thr Glu 
                565                 570                 575     


Asn Gly His Arg Ile Asp Phe His Ser Trp Arg Leu Lys Leu Phe Gly 
            580                 585                 590         


Glu Ser Ile Asp Ser Ser Lys Thr Glu Thr Phe Val Phe Gly Asn Asp 
        595                 600                 605             


Lys Glu Glu Val Glu Pro Ala Ala Thr Glu Ser Thr Val Ser Gln Tyr 
    610                 615                 620                 


Ser Ala Ser Ser Thr Ser Ile Ser Ile Ser Ala Thr Ser Thr Ser Ser 
625                 630                 635                 640 


Ile Ser Ile Gly Val Glu Thr Ser Ala Ile Pro Gln Thr Thr Thr Ala 
                645                 650                 655     


Ser Thr Asp Pro Asp Ser Asp Pro Asn Thr Pro Lys Lys Leu Ser Ser 
            660                 665                 670         


Pro Arg Gln Ala Met His Tyr Phe Leu Thr Ile Phe Leu Ile Gly Ala 
        675                 680                 685             


Thr Phe Leu Val Leu Tyr Phe Met Phe Phe Met Lys Ser Arg Arg Arg 
    690                 695                 700                 


Ile Arg Arg Ser Arg Ala Glu Thr Tyr Glu Phe Asp Ile Ile Asp Thr 
705                 710                 715                 720 


Asp Ser Glu Tyr Asp Ser Thr Leu Asp Asn Gly Thr Ser Gly Ile Thr 
                725                 730                 735     


Glu Pro Glu Glu Val Glu Asp Phe Asp Phe Asp Leu Ser Asp Glu Asp 
            740                 745                 750         


His Leu Ala Ser Leu Ser Ser Ser Glu Asn Gly Asp Ala Glu His Thr 
        755                 760                 765             


Ile Asp Ser Val Leu Thr Asn Glu Asn Pro Phe Ser Asp Pro Ile Lys 
    770                 775                 780                 


Gln Lys Phe Pro Asn Asp Ala Asn Ala Glu Ser Ala Ser Asn Lys Leu 
785                 790                 795                 800 


Gln Glu Leu Gln Pro Asp Val Pro Pro Ser Ser Gly Arg Ser 
                805                 810                 


<210>  29
<211>  116
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer MBP267

<400>  29
atactattgt aattcaaaaa aaaaaagcga atcttcccat gcctgttgct gctcttgaat       60

ggcgacagcc tattgcccca gtgttccctc aacaaccttg cgtacgctgc aggtcg          116


<210>  30
<211>  119
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer MBP268

<400>  30
acagttgtag tcacgtgcgc gccatgctga ctaatggcag ccgtcgttgg gcagaagaga       60

attagtatgg tacaggatac gctaattgcg ctccaactac atcgatgaat tcgagctcg       119


<210>  31
<211>  2723
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-GSH1 5'UTR-KanMX-GSH1 5'UTR-GSH1 ORF fragment. Sequence 
       amplified by MBP288 and MBP289 and used for GSH1 SNP reversion.

<400>  31
gattttatcg gtcaaagggg aaatcaatgc gaaagacagt aatgatgaga gaaaaactct       60

ccgtaaccac caagtttggt tcagcgcgac gagattttta tcgattatcg agaaaaatac      120

ctgtatatct acatttctat gtcagtgata tatacttctt agataagtta tgccaccagt      180

gcatacgctt acgcacacac acgtattctt gtgcacacgc ctgttacttc ttgcagacat      240

cagacatact attgtaattc aaaaaaaaaa agcgaatctt cccatgcctg ttgctgctct      300

tgaatggcga cagcctattg ccccagtgtt ccctcaacaa ccttgcgtac gctgcaggtc      360

gacggatccc cgggttaatt aaggcgcgcc agatctgttt agcttgcctc gtccccgccg      420

ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc      480

agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa      540

tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg      600

ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc      660

gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata      720

cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca taaacaacca      780

tgggtaagga aaagactcac gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt      840

tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat      900

tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca      960

atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga     1020

ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg     1080

gcaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg     1140

cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca     1200

gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg     1260

cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc     1320

ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata     1380

accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg     1440

cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat     1500

tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt     1560

ttcatttgat gctcgatgag tttttctaat cagtactgac aataaaaaga ttcttgtttt     1620

caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt     1680

tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc     1740

gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat     1800

tcgatactaa cgccgccatc cagtgtcgaa aacgagctcg aattcatcga tgtagttgga     1860

gcgcaattag cgtatcctgt accatactaa ttctcttctg cccaacgacg gctgccatta     1920

gtcagcatgg cgcgcacgtg actacaactg tggctggaaa ccttttcgtc ctccccggtt     1980

tttcagtgag ccgactctac tacaatgctt tttcattttt cactcagaaa aacctgcaat     2040

ttgccaaatt ggccatgctc tgtgcctccc ttgacaaagg acatcttccc tgtttataaa     2100

cggcggctta ccaaaagttg aagcttgttc ttgcctctta tgagtggagc aatcgattat     2160

attgaatcgt tgtgctggag tagttggatc tttccacgtg gtctcgagtc acttgtagaa     2220

gctgaaaatt gagcagattt agtatagggc tacattgtag ggtggtttag agtatcgaaa     2280

atatacatat agaagaataa aatgggactc ttagctttgg gcacgccttt gcagtggttt     2340

gagtctagga cgtacaatga acacataagg gatgaaggta tcgagcagtt gttgtatatt     2400

ttccaagctg ctggtaaaag agacaatgac cctctttttt ggggagacga gcttgagtac     2460

atggttgtag attttgatga taaggagaga aattctatgc tcgacgtttg ccatgacaag     2520

atactcactg agcttaatat ggaggattcg tccctttgtg aggctaacga tgtgagtttt     2580

caccctgagt atggccggta tatgttagag gcaacaccag cttctccata tttgaattac     2640

gtgggtagtt acgttgaggt taacatgcaa aaaagacgtg ccattgcaga atataagcta     2700

tctgaatatg cgagacaaga tag                                             2723


<210>  32
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer MBP288

<400>  32
gattttatcg gtcaaagg                                                     18


<210>  33
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer MBP289

<400>  33
ctatcttgtc tcgcatattc                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer MBP290

<400>  34
tctcgagtca cttgtagaag                                                   20


<210>  35
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer  MBP292

<400>  35
gagcccacat gcaagtt                                                      17


<210>  36
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer MBP291

<400>  36
gtagggtggt ttagagtatc                                                   20


<210>  37
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer MBP393

<400>  37
tattatgaat tcaaatgttg agcccgaaga cg                                     32


<210>  38
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer MBP396

<400>  38
tattataagc ttaaattagc gaagcaggtt cc                                     32


<210>  39
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer MBP397

<400>  39
tattatgcat gccacgtatt cttgtgcaca cg                                     32


<210>  40
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer MBP406

<400>  40
tattatgtcg actaccacct acaccaataa gc                                     32


<210>  41
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified invertase signal peptide


<220>
<221>  MISC
<222>  (7)..(7)
<223>  Xaa = Phe, Trp or Tyr

<220>
<221>  MISC
<222>  (8)..(8)
<223>  Xaa = Ile, Leu, Val, Ala or Met

<220>
<221>  MISC
<222>  (9)..(9)
<223>  Xaa = Leu, Val, Ala or Met

<220>
<221>  MISC
<222>  (10)..(10)
<223>  Xaa = Ser or Thr

<220>
<221>  MISC
<222>  (11)..(11)
<223>  Xaa = Ile, Val, Ala or Met

<400>  41

Met Leu Leu Gln Ala Phe Xaa Xaa Xaa Xaa Xaa Gly Phe Ala Ala Lys 
1               5                   10                  15      


Ile Ser Ala 
            


<210>  42
<211>  19
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Modified invertase signal peptide

<400>  42

Met Leu Leu Gln Ala Phe Phe Ile Val Ser Ile Gly Phe Ala Ala Lys 
1               5                   10                  15      


Ile Ser Ala 
            


<210>  43
<211>  2118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KanMX  flanked on the 5' end by 300bp immediately upstream to the
       GSH1 ORF and on the 3' end by 300bp immediately downstream to the
       GSH1 ORF

<400>  43
ggtaccacaa tgctttttca tttttcactc agaaaaacct gcaatttgcc aaattggcca       60

tgctctgtgc ctcccttgac aaaggacatc ttccctgttt ataaacggcg gcttaccaaa      120

agttgaagct tgttcttgcc tcttatgagt ggagcaatcg attatattga atcgttgtgc      180

tggagtagtt ggatctttcc acgtggtctc gagtcacttg tagaagctga aaattgagca      240

gatttagtat agggctacat tgtagggtgg tttagagtat cgaaaatata catatagaag      300

aataaacgta cgctgcaggt cgacggatcc ccgggttaat taaggcgcgc cagatctgtt      360

tagcttgcct cgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc      420

cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag      480

cccatacatc cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga      540

agcaaaaatt acggctcctc gctgcagacc tgcgagcagg gaaacgctcc cctcacagac      600

gcgttgaatt gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca      660

ctgaggttct tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc      720

acatccgaac ataaacaacc atgggtaagg aaaagactca cgtttcgagg ccgcgattaa      780

attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat      840

caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac      900

atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga      960

cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt     1020

tactcaccac tgcgatcccc ggcaaaacag cattccaggt attagaagaa tatcctgatt     1080

caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg     1140

tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa     1200

tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg     1260

aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc     1320

atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg     1380

atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc     1440

tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc     1500

ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagtactga     1560

caataaaaag attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt     1620

tgttctattt taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg     1680

cccagatgcg aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct     1740

ggtcgctata ctgctgtcga ttcgatacta acgccgccat ccagtgtcga aaacgagctc     1800

gaattcatcg atactccttt tacttcggtt gtgaaagaaa gttgacatta tcgatttggg     1860

tgacacggtg attgaaaaag caacgaccag tattatacct ctttttttta ttattcagtt     1920

tatatttttg caagtgatct taagcatttc tacacaaact tatgccaacg tgaccattta     1980

ttattttata tagcaaaaaa aaatgagggg ccttgcagaa caattgttgc gagtttctaa     2040

taacaagcac gtagaatatt ggccatttaa tttttctctt caatttatag aatggttgtg     2100

ttagtgacaa aaggtacc                                                   2118


<210>  44
<211>  1755
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence encoding an albumin variant with mutations 
       E492G, K573P, K574H and Q580K relative to wt HSA

<400>  44
gacgctcaca agtctgaagt tgctcacaga ttcaaggact taggcgagga aaacttcaaa       60

gctttggttt tgattgcatt cgctcaatac ttacaacaat gcccattcga agaccacgtc      120

aaattggtta acgaagttac tgaattcgca aagacttgcg ttgcagacga atccgcagaa      180

aactgcgaca agtctttgca cacattgttc ggcgacaaat tgtgcacagt tgctacttta      240

agagaaacat acggcgaaat ggctgactgc tgcgctaaac aagagccaga aagaaacgaa      300

tgcttcttac aacacaagga cgacaaccca aacttaccaa gattggttag accagaagtt      360

gacgttatgt gcacagcatt ccacgacaac gaagaaactt tcttgaagaa gtacttgtac      420

gaaattgcta gaagacaccc atacttctac gctccagaat tgttgttctt cgctaaaaga      480

tacaaggctg cattcacaga atgctgccaa gcagctgaca aggctgcttg cttgttacca      540

aagttggacg aattgagaga cgaaggcaag gcttcttctg ctaagcaaag gttgaaatgc      600

gcttctttgc aaaagttcgg cgagagagca ttcaaggcat gggctgttgc tcgtttatct      660

caaagattcc caaaagcaga gttcgctgaa gtttccaagt tagttactga cttgacaaaa      720

gttcacactg aatgctgcca cggcgacttg ttagagtgcg ctgacgaccg tgctgactta      780

gccaaataca tttgcgaaaa ccaagactct atttcttcta agttaaagga gtgctgcgaa      840

aaaccgttgt tagagaaatc tcactgcatt gctgaagttg aaaacgacga aatgccagct      900

gacttgccat ctttagctgc tgacttcgtt gaatctaaag acgtttgcaa gaactacgca      960

gaagctaagg acgttttctt gggcatgttc ttatacgaat acgcaagaag acacccagac     1020

tactctgttg ttttgttgtt aagattggct aagacttacg aaactacatt agaaaagtgc     1080

tgcgctgccg cagacccaca cgaatgctac gctaaagttt tcgacgagtt caagccattg     1140

gttgaagaac cacaaaactt gattaagcaa aactgcgagt tattcgaaca attgggcgaa     1200

tacaaattcc aaaacgcctt gttagttaga tacactaaga aagttccaca agtttcaact     1260

ccaacattgg ttgaagtttc tcgtaactta ggcaaggttg gctctaagtg ctgcaaacac     1320

ccagaggcta agcgtatgcc atgcgctgaa gactacttgt ctgttgtttt gaaccagtta     1380

tgcgttttgc acgagaagac tccagtttct gaccgtgtta ctaagtgctg cacagaatct     1440

ttagttaaca gacgtccatg cttctcagct ttgggtgttg acgaaactta cgttccaaaa     1500

gaattcaacg ctgaaacttt cactttccac gctgacattt gcactttgtc tgaaaaggaa     1560

agacagatta agaaacaaac tgctttggtt gaattggtta agcacaagcc aaaggctact     1620

aaggaacaat tgaaggctgt tatggacgac ttcgctgctt tcgttgaaaa gtgctgcaaa     1680

gctgacgaca aggaaacttg cttcgctgag gaaggcccac atttggttgc agcttcaaaa     1740

gctgctttgg gcttg                                                      1755


<210>  45
<211>  585
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Albumin variant with mutations E492G, K573P, K574H and Q580K 
       relative to wt HSA

<400>  45

Asp Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu 
1               5                   10                  15      


Glu Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu Gln 
            20                  25                  30          


Gln Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr Glu 
        35                  40                  45              


Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys 
    50                  55                  60                  


Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu 
65                  70                  75                  80  


Arg Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro 
                85                  90                  95      


Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu 
            100                 105                 110         


Pro Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His 
        115                 120                 125             


Asp Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala Arg 
    130                 135                 140                 


Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys Arg 
145                 150                 155                 160 


Tyr Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala 
                165                 170                 175     


Cys Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser 
            180                 185                 190         


Ser Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Glu 
        195                 200                 205             


Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe Pro 
    210                 215                 220                 


Lys Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys 
225                 230                 235                 240 


Val His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp 
                245                 250                 255     


Arg Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile Ser 
            260                 265                 270         


Ser Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser His 
        275                 280                 285             


Cys Ile Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro Ser 
    290                 295                 300                 


Leu Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala 
305                 310                 315                 320 


Glu Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg 
                325                 330                 335     


Arg His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys Thr 
            340                 345                 350         


Tyr Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu 
        355                 360                 365             


Cys Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro 
    370                 375                 380                 


Gln Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu 
385                 390                 395                 400 


Tyr Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro 
                405                 410                 415     


Gln Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys 
            420                 425                 430         


Val Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys 
        435                 440                 445             


Ala Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu His 
    450                 455                 460                 


Glu Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser 
465                 470                 475                 480 


Leu Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Gly Val Asp Glu Thr 
                485                 490                 495     


Tyr Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp 
            500                 505                 510         


Ile Cys Thr Leu Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr Ala 
        515                 520                 525             


Leu Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln Leu 
    530                 535                 540                 


Lys Ala Val Met Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys Lys 
545                 550                 555                 560 


Ala Asp Asp Lys Glu Thr Cys Phe Ala Glu Glu Gly Pro His Leu Val 
                565                 570                 575     


Ala Ala Ser Lys Ala Ala Leu Gly Leu 
            580                 585 


<210>  46
<211>  2253
<212>  DNA
<213>  Artificial sequence

<220>
<223>  IL-1Ra fused to the C-terminus of HSA

<400>  46
gacgctcaca agtccgaagt cgctcacaga ttcaaggact tgggtgaaga aaacttcaag       60

gctttggtct tgatcgcttt cgctcaatac ttgcaacaat gtccattcga agatcacgtc      120

aagttggtca acgaagttac cgaattcgct aagacttgtg ttgctgacga atccgcggaa      180

aactgtgaca agtccttgca caccttgttc ggtgataagt tgtgtactgt tgctaccttg      240

agagaaacct acggtgaaat ggctgactgt tgtgctaagc aagaaccaga aagaaacgaa      300

tgtttcttgc aacacaagga cgacaaccca aacttgccaa gattggttag accagaagtt      360

gacgtcatgt gtactgcttt ccacgacaac gaagaaacct tcttgaagaa gtacttgtac      420

gaaattgcta gaagacaccc atacttctac gctccagaat tgttgttctt cgctaagaga      480

tacaaggctg ctttcaccga atgttgtcaa gctgctgata aggctgcttg tttgttgcca      540

aagttggatg aattgagaga cgaaggtaag gcttcttccg ctaagcaaag attgaagtgt      600

gcttccttgc aaaagttcgg tgaaagagct ttcaaggctt gggctgtcgc tagattgtct      660

caaagattcc caaaggctga attcgctgaa gtttctaagt tggttactga cttgactaag      720

gttcacactg aatgttgtca cggtgacttg ttggaatgtg ctgatgacag agctgacttg      780

gctaagtaca tctgtgaaaa ccaagactct atctcttcca agttgaagga atgttgtgaa      840

aagccattgt tggaaaagtc tcactgtatt gctgaagttg aaaacgatga aatgccagct      900

gacttgccat ctttggctgc tgacttcgtt gaatctaagg acgtttgtaa gaactacgct      960

gaagctaagg acgtcttctt gggtatgttc ttgtacgaat acgctagaag acacccagac     1020

tactccgttg tcttgttgtt gagattggct aagacctacg aaactaccct cgagaagtgt     1080

tgtgctgctg ctgacccaca cgaatgttac gctaaggttt tcgatgaatt caagccattg     1140

gtcgaagaac cacaaaactt gatcaagcaa aactgtgaat tgttcgaaca attgggtgaa     1200

tacaagttcc aaaacgcttt gttggttaga tacactaaga aggtcccaca agtctccacc     1260

ccaactttgg ttgaagtctc tagaaacttg ggtaaggtcg gttctaagtg ttgtaagcac     1320

ccagaagcta agagaatgcc atgtgctgaa gattacttgt ccgtcgtttt gaaccaattg     1380

tgtgttttgc acgaaaagac cccagtctct gatagagtca ccaagtgttg tactgaatct     1440

ttggttaaca gaagaccatg tttctctgct ttggaagtcg acgaaactta cgttccaaag     1500

gaattcaacg ctgaaacttt caccttccac gctgatatct gtaccttgtc cgaaaaggaa     1560

agacaaatta agaagcaaac tgctttggtt gaattggtca agcacaagcc aaaggctact     1620

aaggaacaat tgaaggctgt catggatgat ttcgctgctt tcgttgaaaa gtgttgtaag     1680

gctgatgata aggaaacttg tttcgctgaa gaaggtaaga agttggtcgc tgcttcccaa     1740

gctgccttag gtttgggtgg ttctggtggt tccggtggtt ctggtggatc cggtggtcga     1800

ccctctggga gaaaatccag caagatgcaa gccttcagaa tctgggatgt taaccagaag     1860

accttctatc tgaggaacaa ccaactagtt gctggatact tgcaaggacc aaatgtcaat     1920

ttagaagaaa agatagatgt ggtacccatt gagcctcatg ctctgttctt gggaatccat     1980

ggagggaaga tgtgcctgtc ctgtgtcaag tctggtgatg agaccagact ccagctggag     2040

gcagttcaaa tcactgacct gagcgagaac agaaagcagg acaagcgctt cgccttcatc     2100

cgctcagaca gcggccccac caccagtttt gagtctgccg cctgccccgg ttggttcctc     2160

tgcacagcga tggaagctga ccagcccgtc agcctcacca atatgcctga cgaaggcgtc     2220

atggtcacca aattctactt ccaggaggac gag                                  2253


<210>  47
<211>  751
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-1Ra fused to the C-terminus of HSA

<400>  47

Asp Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu 
1               5                   10                  15      


Glu Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu Gln 
            20                  25                  30          


Gln Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr Glu 
        35                  40                  45              


Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys 
    50                  55                  60                  


Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu 
65                  70                  75                  80  


Arg Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro 
                85                  90                  95      


Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu 
            100                 105                 110         


Pro Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His 
        115                 120                 125             


Asp Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala Arg 
    130                 135                 140                 


Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys Arg 
145                 150                 155                 160 


Tyr Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala 
                165                 170                 175     


Cys Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser 
            180                 185                 190         


Ser Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Glu 
        195                 200                 205             


Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe Pro 
    210                 215                 220                 


Lys Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys 
225                 230                 235                 240 


Val His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp 
                245                 250                 255     


Arg Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile Ser 
            260                 265                 270         


Ser Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser His 
        275                 280                 285             


Cys Ile Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro Ser 
    290                 295                 300                 


Leu Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala 
305                 310                 315                 320 


Glu Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg 
                325                 330                 335     


Arg His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys Thr 
            340                 345                 350         


Tyr Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu 
        355                 360                 365             


Cys Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro 
    370                 375                 380                 


Gln Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu 
385                 390                 395                 400 


Tyr Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro 
                405                 410                 415     


Gln Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys 
            420                 425                 430         


Val Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys 
        435                 440                 445             


Ala Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu His 
    450                 455                 460                 


Glu Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser 
465                 470                 475                 480 


Leu Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu Thr 
                485                 490                 495     


Tyr Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp 
            500                 505                 510         


Ile Cys Thr Leu Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr Ala 
        515                 520                 525             


Leu Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln Leu 
    530                 535                 540                 


Lys Ala Val Met Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys Lys 
545                 550                 555                 560 


Ala Asp Asp Lys Glu Thr Cys Phe Ala Glu Glu Gly Lys Lys Leu Val 
                565                 570                 575     


Ala Ala Ser Gln Ala Ala Leu Gly Leu Gly Gly Ser Gly Gly Ser Gly 
            580                 585                 590         


Gly Ser Gly Gly Ser Gly Gly Arg Pro Ser Gly Arg Lys Ser Ser Lys 
        595                 600                 605             


Met Gln Ala Phe Arg Ile Trp Asp Val Asn Gln Lys Thr Phe Tyr Leu 
    610                 615                 620                 


Arg Asn Asn Gln Leu Val Ala Gly Tyr Leu Gln Gly Pro Asn Val Asn 
625                 630                 635                 640 


Leu Glu Glu Lys Ile Asp Val Val Pro Ile Glu Pro His Ala Leu Phe 
                645                 650                 655     


Leu Gly Ile His Gly Gly Lys Met Cys Leu Ser Cys Val Lys Ser Gly 
            660                 665                 670         


Asp Glu Thr Arg Leu Gln Leu Glu Ala Val Gln Ile Thr Asp Leu Ser 
        675                 680                 685             


Glu Asn Arg Lys Gln Asp Lys Arg Phe Ala Phe Ile Arg Ser Asp Ser 
    690                 695                 700                 


Gly Pro Thr Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly Trp Phe Leu 
705                 710                 715                 720 


Cys Thr Ala Met Glu Ala Asp Gln Pro Val Ser Leu Thr Asn Met Pro 
                725                 730                 735     


Asp Glu Gly Val Met Val Thr Lys Phe Tyr Phe Gln Glu Asp Glu 
            740                 745                 750     


<210>  48
<211>  756
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FLAG fused to the C-terminus of scFv

<400>  48
gaagttcaat tgttggaatc tggtggtggt ttggttcaac ctggtggttc tttgagattg       60

tcttgtgctg cttctggttt tactttttct aattattgga tgtcttgggt tagacaagct      120

ccaggtaaag gtttggaatg ggtttccggt atttcaggta atggtggtta tacttatttt      180

gctgattcag ttaaagatag atttactatt tctagagata attctaaaaa taccttatat      240

ttgcaaatga actctttgag agcagaagat actgctgttt attactgtgc aggtggtgac      300

ggttctggtt ggagtttttg gggtcaaggt actctagtta ccgtttcttc aggtggtggt      360

ggttctggtg gaggtggatc aggtggtgga ggatctcaat cagttttgac tcaaccacca      420

tctgcttcag gtactccagg tcaaagagtt accatttctt gtactggttc ttcttctaat      480

attggtgcag gttacgatgt tcattggtat caacaattgc caggtactgc tccaaaattg      540

ttgatttatg gtaacaacaa tagaccatct ggtgtcccag atagattttc tggttctaaa      600

tctggtactt ctgcttcttt ggctatttct ggtttaagat cagaagatga agctgattac      660

tactgtgctg cttgggatga ctctttgtct ggtagagttt tcggtggtgg tactaaattg      720

accgttttgg gtgattataa agatgatgac gataaa                                756


<210>  49
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLAG fused to the C-terminus of scFv

<400>  49

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Gly Ile Ser Gly Asn Gly Gly Tyr Thr Tyr Phe Ala Asp Ser Val 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Gly Gly Asp Gly Ser Gly Trp Ser Phe Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly 
    130                 135                 140                 


Thr Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn 
145                 150                 155                 160 


Ile Gly Ala Gly Tyr Asp Val His Trp Tyr Gln Gln Leu Pro Gly Thr 
                165                 170                 175     


Ala Pro Lys Leu Leu Ile Tyr Gly Asn Asn Asn Arg Pro Ser Gly Val 
            180                 185                 190         


Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala 
        195                 200                 205             


Ile Ser Gly Leu Arg Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala 
    210                 215                 220                 


Trp Asp Asp Ser Leu Ser Gly Arg Val Phe Gly Gly Gly Thr Lys Leu 
225                 230                 235                 240 


Thr Val Leu Gly Asp Tyr Lys Asp Asp Asp Asp Lys 
                245                 250         


<210>  50
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FLAG

<400>  50

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  51
<211>  30
<212>  PRT
<213>  Artificial sequence

<220>
<223>  GLP-1 fragment

<400>  51

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg 
            20                  25                  30  


<210>  52
<211>  60
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Tandem repeat of GLP-1 fragment

<400>  52

His Gly Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg His Gly 
            20                  25                  30          


Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala 
        35                  40                  45              


Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg 
    50                  55                  60  


