                         SEQUENCE LISTING

<110>  Conagen Inc.
 
<120>  HYDROLYSIS OF STEVIOL GLYCOSIDES BY BETA-GLUCOSIDASE

<130>  C1497.70026WO00

<140>  Not Yet Assigned
<141>  Concurrently Herewith

<150>  US 62/527,482
<151>  2017-06-30

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  839
<212>  PRT
<213>  Pichia pastoris

<400>  1

Met Thr Gln Leu Asp Val Glu Ser Leu Ile Gln Glu Leu Thr Leu Asn 
1               5                   10                  15      


Glu Lys Val Gln Leu Leu Ser Gly Ser Asp Phe Trp His Thr Thr Pro 
            20                  25                  30          


Val Arg Arg Leu Gly Ile Pro Lys Met Arg Leu Ser Asp Gly Pro Asn 
        35                  40                  45              


Gly Val Arg Gly Thr Lys Phe Phe Asn Gly Val Pro Thr Ala Cys Phe 
    50                  55                  60                  


Pro Cys Gly Thr Gly Leu Gly Ala Thr Phe Asp Lys Glu Leu Leu Lys 
65                  70                  75                  80  


Glu Ala Gly Ser Leu Met Ala Asp Glu Ala Lys Ala Lys Ala Ala Ser 
                85                  90                  95      


Val Val Leu Gly Pro Thr Ala Asn Ile Ala Arg Gly Pro Asn Gly Gly 
            100                 105                 110         


Arg Gly Phe Glu Ser Phe Gly Glu Asp Pro Val Val Asn Gly Leu Ser 
        115                 120                 125             


Ser Ala Ala Met Ile Asn Gly Leu Gln Gly Lys Tyr Ile Ala Ala Thr 
    130                 135                 140                 


Met Lys His Tyr Val Cys Asn Asp Leu Glu Met Asp Arg Asn Cys Ile 
145                 150                 155                 160 


Asp Ala Gln Val Ser His Arg Ala Leu Arg Glu Val Tyr Leu Leu Pro 
                165                 170                 175     


Phe Gln Ile Ala Val Arg Asp Ala Asn Pro Arg Ala Ile Met Thr Ala 
            180                 185                 190         


Tyr Asn Lys Ala Asn Gly Glu His Val Ser Gln Ser Lys Phe Leu Leu 
        195                 200                 205             


Asp Glu Val Leu Arg Lys Glu Trp Gly Trp Asp Gly Leu Leu Met Ser 
    210                 215                 220                 


Asp Trp Phe Gly Val Tyr Asp Ala Lys Ser Ser Ile Thr Asn Gly Leu 
225                 230                 235                 240 


Asp Leu Glu Met Pro Gly Pro Pro Gln Cys Arg Val His Ser Ala Thr 
                245                 250                 255     


Asp His Ala Ile Asn Ser Gly Glu Ile His Ile Asn Asp Val Asp Glu 
            260                 265                 270         


Arg Val Arg Ser Leu Leu Ser Leu Ile Asn Tyr Cys His Gln Ser Gly 
        275                 280                 285             


Val Thr Glu Glu Asp Pro Glu Thr Ser Asp Asn Asn Thr Pro Glu Thr 
    290                 295                 300                 


Ile Glu Lys Leu Arg Lys Ile Ser Arg Glu Ser Ile Val Leu Leu Lys 
305                 310                 315                 320 


Asp Asp Asp Arg Asn Arg Ser Ile Leu Pro Leu Lys Lys Ser Asp Lys 
                325                 330                 335     


Ile Ala Val Ile Gly Asn Asn Ala Lys Gln Ala Ala Tyr Cys Gly Gly 
            340                 345                 350         


Gly Ser Ala Ser Val Leu Ser Tyr His Thr Thr Thr Pro Phe Asp Ser 
        355                 360                 365             


Ile Lys Ser Arg Leu Glu Asp Ser Asn Thr Pro Ala Tyr Thr Ile Gly 
    370                 375                 380                 


Ala Asp Ala Tyr Lys Asn Leu Pro Pro Leu Gly Pro Gln Met Thr Asp 
385                 390                 395                 400 


Ser Asp Gly Lys Pro Gly Phe Asp Ala Lys Phe Phe Val Gly Ser Pro 
                405                 410                 415     


Thr Ser Lys Asp Arg Lys Leu Ile Asp His Phe Gln Leu Thr Asn Ser 
            420                 425                 430         


Gln Val Phe Leu Val Asp Tyr Tyr Asn Glu Gln Ile Pro Glu Asn Lys 
        435                 440                 445             


Glu Phe Tyr Val Asp Val Glu Gly Gln Phe Ile Pro Glu Glu Asp Gly 
    450                 455                 460                 


Thr Tyr Asn Phe Gly Leu Thr Val Phe Gly Thr Gly Arg Leu Phe Val 
465                 470                 475                 480 


Asp Asp Lys Leu Val Ser Asp Ser Ser Gln Asn Gln Thr Pro Gly Asp 
                485                 490                 495     


Ser Phe Phe Gly Leu Ala Ala Gln Glu Val Ile Gly Ser Ile His Leu 
            500                 505                 510         


Val Lys Gly Lys Ala Tyr Lys Ile Lys Val Leu Tyr Gly Ser Ser Val 
        515                 520                 525             


Thr Arg Thr Tyr Glu Ile Ala Ala Ser Val Ala Phe Glu Gly Gly Ala 
    530                 535                 540                 


Phe Thr Phe Gly Ala Ala Lys Gln Arg Asn Glu Asp Glu Glu Ile Ala 
545                 550                 555                 560 


Arg Ala Val Glu Ile Ala Lys Ala Asn Asp Lys Val Val Leu Cys Ile 
                565                 570                 575     


Gly Leu Asn Gln Asp Phe Glu Ser Glu Gly Phe Asp Arg Pro Asp Ile 
            580                 585                 590         


Lys Ile Pro Gly Ala Thr Asn Lys Met Val Ser Ala Val Leu Lys Ala 
        595                 600                 605             


Asn Pro Asn Thr Val Ile Val Asn Gln Thr Gly Thr Pro Val Glu Met 
    610                 615                 620                 


Pro Trp Ala Ser Asp Ala Pro Val Ile Leu Gln Ala Trp Phe Gly Gly 
625                 630                 635                 640 


Ser Glu Ala Gly Thr Ala Ile Ala Asp Val Leu Phe Gly Asp Tyr Asn 
                645                 650                 655     


Pro Ser Gly Lys Leu Thr Val Thr Phe Pro Leu Arg Phe Glu Asp Asn 
            660                 665                 670         


Pro Ala Tyr Leu Asn Phe Gln Ser Asn Lys Gln Ala Cys Trp Tyr Gly 
        675                 680                 685             


Glu Asp Val Tyr Val Gly Tyr Arg Tyr Tyr Glu Thr Ile Asp Arg Pro 
    690                 695                 700                 


Val Leu Phe Pro Phe Gly His Gly Leu Ser Phe Thr Glu Phe Asp Phe 
705                 710                 715                 720 


Thr Asp Met Phe Val Arg Leu Glu Glu Glu Asn Leu Glu Val Glu Val 
                725                 730                 735     


Val Val Arg Asn Thr Gly Lys Tyr Asp Gly Ala Glu Val Val Gln Leu 
            740                 745                 750         


Tyr Val Ala Pro Val Ser Pro Ser Leu Lys Arg Pro Ile Lys Glu Leu 
        755                 760                 765             


Lys Glu Tyr Ala Lys Ile Phe Leu Ala Ser Gly Glu Ala Lys Thr Val 
    770                 775                 780                 


His Leu Ser Val Pro Ile Lys Tyr Ala Thr Ser Phe Phe Asp Glu Tyr 
785                 790                 795                 800 


Gln Lys Lys Trp Cys Ser Glu Lys Gly Glu Tyr Thr Ile Leu Leu Gly 
                805                 810                 815     


Ser Ser Ser Ala Asp Ile Lys Val Ser Gln Ser Ile Thr Leu Glu Lys 
            820                 825                 830         


Thr Thr Phe Trp Lys Gly Leu 
        835                 


<210>  2
<211>  2520
<212>  DNA
<213>  Artificial Seq

<220>
<223>  Synthetic polynucleotide

<400>  2
atgacccaac tggatgtgga gagcctgatt caagagctga ccctgaacga aaaggtgcaa       60

ctgctgagcg gtagcgactt ctggcatacc accccggttc gtcgtctggg catcccgaag      120

atgcgtctga gcgacggtcc gaacggcgtt cgtggtacca aattctttaa cggtgttccg      180

accgcgtgct tcccgtgcgg taccggtctg ggcgcgacct ttgacaagga actgctgaaa      240

gaggcgggta gcctgatggc ggatgaagcg aaagcgaaag cggcgagcgt ggttctgggt      300

ccgaccgcga acattgcgcg tggtccgaac ggtggccgtg gcttcgagag cttcggcgag      360

gacccggtgg ttaacggtct gagcagcgcg gcgatgatca acggcctgca gggcaagtac      420

attgcggcga ccatgaaaca ctatgtttgc aacgatctgg aaatggaccg taactgcatt      480

gacgcgcaag ttagccaccg tgcgctgcgt gaggtgtacc tgctgccgtt ccaaatcgcg      540

gtgcgtgatg cgaacccgcg tgcgattatg accgcgtata acaaggcgaa cggcgaacac      600

gttagccaga gcaaattcct gctggacgaa gtgctgcgta aggagtgggg ctgggatggt      660

ctgctgatga gcgactggtt tggtgtttac gatgcgaaaa gcagcatcac caacggcctg      720

gacctggaga tgccgggtcc gccgcagtgc cgtgtgcaca gcgcgaccga tcacgcgatc      780

aacagcggcg aaatccacat taacgatgtt gacgagcgtg tgcgtagcct gctgagcctg      840

attaactact gccaccaaag cggtgttacc gaggaagatc cggaaaccag cgacaacaac      900

accccggaaa ccatcgagaa gctgcgtaaa atcagccgtg agagcattgt gctgctgaag      960

gacgatgacc gtaaccgtag cattctgccg ctgaagaaaa gcgacaaaat cgcggttatt     1020

ggtaacaacg cgaaacaagc ggcgtattgc ggtggcggta gcgcgagcgt gctgagctat     1080

cacaccacca ccccgttcga cagcatcaag agccgtctgg aagatagcaa caccccggcg     1140

tacaccattg gtgcggacgc gtataaaaac ctgccgccgc tgggtccgca aatgaccgat     1200

agcgacggca agccgggttt tgatgcgaaa ttctttgttg gcagcccgac cagcaaggat     1260

cgtaaactga tcgaccactt ccagctgacc aacagccaag tttttctggt ggactactat     1320

aacgaacaga tcccggaaaa caaggagttc tacgttgacg tggagggtca atttattccg     1380

gaggaagatg gcacctataa cttcggtctg accgtgtttg gtaccggccg tctgttcgtt     1440

gatgacaaac tggttagcga cagcagccag aaccaaaccc cgggcgatag cttctttggt     1500

ctggcggcgc aggaagtgat cggcagcatt cacctggtga agggtaaagc gtacaagatc     1560

aaagttctgt atggcagcag cgtgacccgt acctacgaaa ttgcggcgag cgttgcgttt     1620

gagggcggtg cgttcacctt tggtgcggcg aaacagcgta acgaagacga ggaaatcgcg     1680

cgtgcggtgg agattgcgaa ggcgaacgac aaagtggttc tgtgcatcgg cctgaaccaa     1740

gatttcgaaa gcgagggttt tgatcgtccg gacatcaaga ttccgggcgc gaccaacaaa     1800

atggttagcg cggtgctgaa ggcgaacccg aacaccgtta ttgtgaacca gaccggtacc     1860

ccggttgaga tgccgtgggc gagcgatgcg ccggtgatcc tgcaagcgtg gtttggcggt     1920

agcgaggcgg gtaccgcgat tgcggatgtt ctgtttggcg actacaaccc gagcggcaag     1980

ctgaccgtga ccttcccgct gcgttttgag gataacccgg cgtacctgaa cttccagagc     2040

aacaaacaag cgtgctggta tggcgaagac gtttacgtgg gttatcgtta ctatgagacc     2100

atcgatcgtc cggtgctgtt cccgtttggt cacggcctga gcttcaccga gttcgatttt     2160

accgacatgt ttgttcgtct ggaggaagag aacctggaag ttgaggtggt tgtgcgtaac     2220

accggcaagt acgacggtgc ggaagtggtg cagctgtatg ttgcgccggt tagcccgagc     2280

ctgaaacgtc cgatcaagga actgaaagag tacgcgaaaa ttttcctggc gagcggtgaa     2340

gcgaagaccg ttcacctgag cgtgccgatc aaatacgcga ccagcttctt tgatgagtat     2400

caaaagaaat ggtgcagcga aaagggcgag tataccattc tgctgggtag cagcagcgcg     2460

gacatcaaag ttagccaaag catcaccctg gaaaaaacca ccttctggaa aggtctgtaa     2520


<210>  3
<211>  346
<212>  PRT
<213>  Pichia pastoris

<400>  3

Met Lys Ser Gln Leu Ile Phe Met Ala Leu Ala Ser Leu Val Ala Ser 
1               5                   10                  15      


Ala Pro Leu Glu His Gln Gln Gln His His Lys His Glu Lys Arg Ala 
            20                  25                  30          


Val Val Thr Gln Thr Val Thr Val Ala Ala Gly Gln Thr Ala Ala Ala 
        35                  40                  45              


Gly Ser Ala Gln Ala Val Val Thr Ser Ser Ala Ala Pro Ala Ser Val 
    50                  55                  60                  


Ala Ser Ser Ala Ala Ala Ser Ala Ser Ser Ser Ser Ser Ser Tyr Thr 
65                  70                  75                  80  


Ser Gly Ala Ser Gly Asp Leu Ser Ser Phe Lys Asp Gly Thr Ile Lys 
                85                  90                  95      


Cys Ser Glu Phe Pro Ser Gly Asp Gly Val Val Ser Val Ser Trp Leu 
            100                 105                 110         


Gly Phe Gly Gly Trp Ser Ser Ile Met Asn Leu Gln Gly Gly Thr Ser 
        115                 120                 125             


Glu Ser Cys Glu Asn Gly Tyr Tyr Cys Ser Tyr Ala Cys Glu Ala Gly 
    130                 135                 140                 


Tyr Ser Lys Thr Gln Trp Pro Ser Asn Gln Pro Ser Asp Gly Arg Ser 
145                 150                 155                 160 


Val Gly Gly Leu Leu Cys Lys Asp Gly Leu Leu Tyr Arg Ser Asn Thr 
                165                 170                 175     


Ala Phe Asp Thr Leu Cys Val Pro Gly Lys Gly Thr Ala Ser Val Glu 
            180                 185                 190         


Asn Asn Val Ser Lys Gly Ile Ser Ile Cys Arg Thr Asp Tyr Pro Gly 
        195                 200                 205             


Ser Glu Asn Met Cys Val Pro Thr Trp Val Asp Ala Gly Asn Ser Asn 
    210                 215                 220                 


Thr Leu Thr Val Val Asp Glu Asp Asn Tyr Tyr Glu Trp Gln Gly Leu 
225                 230                 235                 240 


Lys Thr Ser Ala Gln Tyr Tyr Val Asn Asn Ala Gly Val Ser Val Glu 
                245                 250                 255     


Asp Gly Cys Ile Trp Gly Asp Glu Ser Ser Gly Val Gly Asn Trp Ala 
            260                 265                 270         


Pro Leu Val Leu Gly Ala Gly Ser Thr Gly Gly Leu Thr Tyr Leu Ser 
        275                 280                 285             


Leu Ile Pro Asn Pro Asn Asn Lys Lys Ala Pro Asn Phe Asn Val Lys 
    290                 295                 300                 


Ile Val Ala Thr Asp Gly Ser Ser Ile Asn Gly Asp Cys Lys Tyr Glu 
305                 310                 315                 320 


Asn Gly Ile Phe Val Gly Ser Ser Thr Asp Gly Cys Thr Val Thr Val 
                325                 330                 335     


Thr Ser Gly Ser Ala Lys Leu Val Phe Tyr 
            340                 345     


<210>  4
<211>  1041
<212>  DNA
<213>  Pichia pastoris

<400>  4
atgaaaagcc agctgatctt tatggctttg gcctcccttg tagcaagtgc accgctggaa       60

caccagcagc agcatcataa acatgagaaa cgcgccgtag ttacgcagac agtaactgtt      120

gcggcgggcc agacagcagc agcgggttcc gcccaggcag ttgttacctc aagcgcggcg      180

ccagcatccg ttgcttcaag tgcggccgcg tctgctagct catcttcttc cagctatacc      240

tctggcgctt caggcgatct tagtagtttc aaagatggta ctattaaatg ttcagaattc      300

ccatcagggg atggcgtggt gtccgtctct tggttaggct tcggcggctg gtctagtatt      360

atgaatctgc agggtggtac ttcagagagt tgtgagaacg gctattattg ttcatatgca      420

tgtgaagccg gttatagcaa aacacagtgg ccatctaacc agccgtcaga tgggagatca      480

gtgggagggt tgctgtgtaa agatggcctg ttatatcgct ccaatacagc gttcgataca      540

ttatgtgtgc ctggaaaagg tacagcatcc gtggagaata atgtgtctaa aggtatttcc      600

atttgtagaa cggattatcc ggggtctgaa aacatgtgcg tcccgacgtg ggtcgatgcc      660

ggtaactcaa acaccttgac agtggtagat gaagataatt attatgaatg gcagggcctt      720

aaaactagtg ctcagtatta tgtgaataac gccggtgtta gtgttgaaga tgggtgcatc      780

tggggcgatg agtccagcgg cgttggaaac tgggcgccgt tggttttggg ggccggttcc      840

acggggggtc tgacctatct gtctctgatt ccgaatccaa acaacaaaaa agcaccgaat      900

tttaacgtaa aaatcgtggc cacggatgga agttcaatta acggagattg caaatatgaa      960

aatgggatct ttgtcggttc ttcaaccgat ggctgcacgg taactgttac ctcaggtagt     1020

gcaaaactgg ttttttatta a                                               1041


<210>  5
<211>  348
<212>  PRT
<213>  Pichia pastoris

<400>  5

Met Gln Val Lys Ser Ile Val Asn Leu Leu Leu Ala Cys Ser Leu Ala 
1               5                   10                  15      


Val Ala Arg Pro Leu Glu His Ala His His Gln His Asp Lys Arg Gly 
            20                  25                  30          


Val Val Val Val Thr Lys Thr Ile Val Val Asp Gly Ser Thr Val Glu 
        35                  40                  45              


Ala Thr Ala Ala Ala Gln Val Gln Glu His Ala Glu Thr Phe Ala Glu 
    50                  55                  60                  


Ser Thr Pro Ser Ala Val Val Ser Ser Ser Ser Ala Pro Ser Ser Ala 
65                  70                  75                  80  


Ser Ser Ala Ser Ala Pro Ala Ser Ser Gly Ser Phe Ser Ala Gly Thr 
                85                  90                  95      


Lys Gly Val Thr Tyr Ser Pro Tyr Gln Ala Gly Gly Gly Cys Lys Thr 
            100                 105                 110         


Ala Glu Glu Val Ala Ser Asp Leu Ser Gln Leu Thr Gly Tyr Glu Ile 
        115                 120                 125             


Ile Arg Leu Tyr Gly Val Asp Cys Asn Gln Val Glu Asn Val Phe Lys 
    130                 135                 140                 


Ala Lys Ala Pro Gly Gln Lys Leu Phe Leu Gly Ile Phe Phe Val Asp 
145                 150                 155                 160 


Ala Ile Glu Ser Gly Val Ser Ala Ile Ala Ser Ala Val Lys Ser Tyr 
                165                 170                 175     


Gly Ser Trp Asp Asp Val His Thr Val Ser Val Gly Asn Glu Leu Val 
            180                 185                 190         


Asn Asn Gly Glu Ala Thr Val Ser Gln Ile Gly Gln Tyr Val Ser Thr 
        195                 200                 205             


Ala Lys Ser Ala Leu Arg Ser Ala Gly Phe Thr Gly Pro Val Leu Ser 
    210                 215                 220                 


Val Asp Thr Phe Ile Ala Val Ile Asn Asn Pro Gly Leu Cys Asp Phe 
225                 230                 235                 240 


Ala Asp Glu Tyr Val Ala Val Asn Ala His Ala Phe Phe Asp Gly Gly 
                245                 250                 255     


Ile Ala Ala Ser Gly Ala Gly Asp Trp Ala Ala Glu Gln Ile Gln Arg 
            260                 265                 270         


Val Ser Ser Ala Cys Gly Gly Lys Asp Val Leu Ile Val Glu Ser Gly 
        275                 280                 285             


Trp Pro Ser Lys Gly Asp Thr Asn Gly Ala Ala Val Pro Ser Lys Ser 
    290                 295                 300                 


Asn Gln Gln Ala Ala Val Gln Ser Leu Gly Gln Lys Ile Gly Ser Ser 
305                 310                 315                 320 


Cys Ile Ala Phe Asn Ala Phe Asn Asp Tyr Trp Lys Ala Asp Gly Pro 
                325                 330                 335     


Phe Asn Ala Glu Lys Tyr Trp Gly Ile Leu Asp Ser 
            340                 345             


<210>  6
<211>  1047
<212>  DNA
<213>  Pichia pastoris

<400>  6
atgcaggtta aatctattgt taatttactg cttgcctgtt ccttggctgt ggcgcgtccg       60

ttggaacacg ctcaccatca gcatgataaa cgcggcgttg tagtagtaac gaaaaccatc      120

gtcgttgatg gtagcacagt tgaggctacc gccgctgctc aggtgcagga gcatgcagaa      180

acctttgcag aatcaacccc gtcagccgtc gtttccagtt catccgcccc ttcatcagca      240

agctcagctt ccgctccagc tagttcaggt tctttttcag ctggtaccaa aggcgtgaca      300

tattctccat atcaggccgg tggtgggtgt aaaacagcgg aagaagtggc atccgatctg      360

tcacagctta ccggttatga aattattcgg ctttatggcg tagattgcaa ccaggttgag      420

aacgtgttta aagccaaagc ccctggccag aaactttttt tgggtatctt ttttgtggat      480

gccatcgagt ctggcgtatc agctatcgca agtgccgtta aatcctatgg ttcttgggat      540

gatgtacaca ctgtatctgt tggcaacgag ctggtgaaca atggcgaagc cactgttagc      600

cagattggac agtatgttag tacggccaaa tcagccttac gctctgccgg tttcacaggg      660

ccagtattgt ctgttgatac ttttattgca gtgattaaca atccggggct gtgtgatttc      720

gcggatgaat atgttgctgt gaacgcccat gcgttcttcg atgggggtat tgctgcctca      780

ggggcgggcg attgggcggc agagcagatc cagcgcgtct ccagtgcgtg cggcgggaaa      840

gatgtcttaa ttgtagaaag cggttggccg tctaaaggag atacgaacgg cgccgcagtg      900

ccgtcaaaat ccaatcagca ggctgcagtc cagagtcttg gccagaaaat tgggagctca      960

tgcattgcct ttaacgcatt taatgattat tggaaagccg atggtccgtt caacgccgaa     1020

aaatattggg ggatccttga tagttaa                                         1047


<210>  7
<211>  464
<212>  PRT
<213>  Pichia pastoris

<400>  7

Met Leu Ser Thr Ile Leu Asn Ile Phe Ile Leu Leu Leu Phe Ile Gln 
1               5                   10                  15      


Ala Ser Leu Gln Ala Pro Ile Pro Val Val Thr Lys Tyr Val Thr Glu 
            20                  25                  30          


Gly Ile Ala Val Val Thr Glu Thr Asn Val Arg Val Val Thr Lys Thr 
        35                  40                  45              


Ile Pro Ile Val Gln Val Leu Ile Ser Asp Gly Ala Thr Tyr Thr His 
    50                  55                  60                  


Thr Leu Thr Thr Val Ser Thr Ala Glu Glu Asn Gly Asn Phe Gln Pro 
65                  70                  75                  80  


Ile Thr Thr Thr Ser Ile Val Asn Lys Glu Val Val Val Pro Thr Ser 
                85                  90                  95      


Val Thr Pro Asn Thr Gln Gln Thr Arg Pro Thr Gln Val Asp Thr Thr 
            100                 105                 110         


Gln Asn Asn Ala Asp Thr Pro Ala Ala Pro Thr Pro Ser Pro Thr Thr 
        115                 120                 125             


Ser Ser Asn Asn Gly Val Phe Thr Thr Tyr Ser Thr Thr Arg Ser Val 
    130                 135                 140                 


Val Thr Ser Val Val Val Val Gly Pro Asp Gly Ser Pro Ile Glu Asn 
145                 150                 155                 160 


Thr Gly Gln Thr Ala Asn Pro Thr Thr Thr Ala Pro Thr Thr Ser Thr 
                165                 170                 175     


Thr Ala Ala Arg Thr Thr Ser Ser Thr Ser Thr Thr Pro Thr Ala Ser 
            180                 185                 190         


Ser Thr Pro Gly Gly Asn His Pro Arg Ser Ile Val Tyr Ser Pro Tyr 
        195                 200                 205             


Ser Asp Ser Ser Gln Cys Lys Asp Ala Thr Thr Ile Glu Thr Asp Leu 
    210                 215                 220                 


Glu Phe Ile Ala Ser Lys Gly Ile Ser Ala Val Arg Ile Tyr Gly Asn 
225                 230                 235                 240 


Asp Cys Asn Tyr Leu Thr Val Val Leu Pro Lys Cys Ala Ser Leu Gly 
                245                 250                 255     


Leu Lys Val Asn Gln Gly Phe Trp Ile Gly Pro Ser Gly Val Asp Ser 
            260                 265                 270         


Ile Asp Asp Ala Val Gln Glu Phe Ile Gln Ala Val Asn Gly Asn Asn 
        275                 280                 285             


Gly Phe Asn Trp Asp Leu Phe Glu Leu Ile Thr Val Gly Asn Glu Ala 
    290                 295                 300                 


Ile Ser Ala Gly Tyr Val Ser Ala Ser Ser Leu Ile Ser Lys Ile Lys 
305                 310                 315                 320 


Glu Val Ser Ser Ile Leu Ser Ser Ala Gly Tyr Thr Gly Pro Ile Thr 
                325                 330                 335     


Thr Ala Glu Pro Pro Asn Val Tyr Glu Asp Tyr Gly Asp Leu Cys Ser 
            340                 345                 350         


Thr Asp Val Met Ser Ile Val Gly Val Asn Ala His Ser Tyr Phe Asn 
        355                 360                 365             


Thr Leu Phe Ala Ala Ser Asp Ser Gly Ser Phe Val Lys Ser Gln Ile 
    370                 375                 380                 


Glu Val Val Gln Lys Ala Cys Ser Arg Ser Asp Ile Thr Ile Ile Glu 
385                 390                 395                 400 


Thr Gly Tyr Pro Ser Gln Gly Ala Thr Asn Gly Lys Asn Val Pro Ser 
                405                 410                 415     


Lys Glu Asn Gln Lys Thr Ala Ile Phe Ser Ile Phe Glu Val Val Gly 
            420                 425                 430         


Thr Asp Val Thr Ile Leu Ser Thr Tyr Asp Asp Leu Trp Lys Asp Pro 
        435                 440                 445             


Gly Pro Tyr Gly Ile Glu Gln Phe Phe Gly Ala Ile Asp Leu Phe Ser 
    450                 455                 460                 


<210>  8
<211>  1395
<212>  DNA
<213>  Pichia pastoris

<400>  8
atgctgtcca caattctgaa tatttttatt cttctgttat tcatccaggc gtctcttcag       60

gcgcctattc cggtggtgac caaatatgtg accgaaggta ttgccgttgt gactgaaacc      120

aatgtgcggg ttgttactaa aaccattccg attgtgcagg tgctgatctc cgatggtgca      180

acctatactc ataccctgac gacagtgtca acggcggaag aaaatggcaa cttccagcct      240

attaccacga catctattgt caacaaagaa gttgtagtac caacaagcgt aaccccgaat      300

acccagcaga cgcgtccgac ccaggtagat accacacaga acaatgcgga tacaccagcg      360

gcgcctacac catcacctac tactagttca aacaacggcg tgttcaccac atattccaca      420

acacgtagcg tagtcactag tgtagtcgta gtcggaccgg atggaagccc tattgaaaat      480

actggacaga cagcaaaccc tactacaact gccccaacta caagcactac tgctgcccgg      540

accacaagca gtacgtccac cacacctacc gctagctcta cgccaggagg taatcatcca      600

cgtagcatcg tctattctcc atattccgat agcagtcagt gtaaagatgc gacaacgatc      660

gaaaccgatc ttgagttcat tgcctctaaa ggcatcagcg cggtacgtat ttatggcaat      720

gattgtaact atcttacagt tgttttgcct aaatgtgcca gtctgggatt aaaagtgaat      780

cagggctttt ggattggtcc aagtggagta gatagcatcg atgatgcagt acaggagttt      840

attcaggcag tcaacggcaa caacggcttt aattgggatt tattcgaatt aattaccgtc      900

ggaaacgaag caatcagtgc cggttatgtt tcagcgagct ccctgatttc caaaattaaa      960

gaagtatcta gcattctgag ctccgcgggt tatactggtc caattaccac agccgaaccg     1020

cctaacgtat atgaggatta tggcgatctg tgctcaaccg atgtaatgtc catcgtgggt     1080

gtaaacgcgc attcctattt taataccctt tttgcggcct ccgattcagg ttcatttgtg     1140

aaatcacaga tcgaagtagt ccagaaagca tgctcacgtt ccgatattac tattattgaa     1200

accgggtatc cgtcccaggg agctaccaat ggaaaaaacg ttcctagtaa agagaatcag     1260

aaaacagcga ttttttcaat ctttgaggtc gttggaacag atgtaactat tcttagtact     1320

tatgatgatt tgtggaaaga tcctggaccg tatgggattg aacagttttt tggtgcgatc     1380

gatctttttt cttaa                                                      1395


