                               SEQUENCE LISTING

<110> THE JOHNS HOPKINS UNIVERSITY
 
<120> A PROTEOLIPOSOME-BASED ZNT8 SELF-ANTIGEN FOR TYPE 1 DIABETES 
      DIAGNOSIS

<130> P14838-02

<140>
<141>

<150> 62/531,525
<151> 2017-07-12

<160> 4     

<170> PatentIn version 3.5

<210> 1
<211> 369
<212> PRT
<213> Homo sapiens

<400> 1
Met Glu Phe Leu Glu Arg Thr Tyr Leu Val Asn Asp Lys Ala Ala Lys 
1               5                   10                  15      


Met Tyr Ala Phe Thr Leu Glu Ser Val Glu Leu Gln Gln Lys Pro Val 
            20                  25                  30          


Asn Lys Asp Gln Cys Pro Arg Glu Arg Pro Glu Glu Leu Glu Ser Gly 
        35                  40                  45              


Gly Met Tyr His Cys His Ser Gly Ser Lys Pro Thr Glu Lys Gly Ala 
    50                  55                  60                  


Asn Glu Tyr Ala Tyr Ala Lys Trp Lys Leu Cys Ser Ala Ser Ala Ile 
65                  70                  75                  80  


Cys Phe Ile Phe Met Ile Ala Glu Val Val Gly Gly His Ile Ala Gly 
                85                  90                  95      


Ser Leu Ala Val Val Thr Asp Ala Ala His Leu Leu Ile Asp Leu Thr 
            100                 105                 110         


Ser Phe Leu Leu Ser Leu Phe Ser Leu Trp Leu Ser Ser Lys Pro Pro 
        115                 120                 125             


Ser Lys Arg Leu Thr Phe Gly Trp His Arg Ala Glu Ile Leu Gly Ala 
    130                 135                 140                 


Leu Leu Ser Ile Leu Cys Ile Trp Val Val Thr Gly Val Leu Val Tyr 
145                 150                 155                 160 


Leu Ala Cys Glu Arg Leu Leu Tyr Pro Asp Tyr Gln Ile Gln Ala Thr 
                165                 170                 175     


Val Met Ile Ile Val Ser Ser Cys Ala Val Ala Ala Asn Ile Val Leu 
            180                 185                 190         


Thr Val Val Leu His Gln Arg Cys Leu Gly His Asn His Lys Glu Val 
        195                 200                 205             


Gln Ala Asn Ala Ser Val Arg Ala Ala Phe Val His Ala Leu Gly Asp 
    210                 215                 220                 


Leu Phe Gln Ser Ile Ser Val Leu Ile Ser Ala Leu Ile Ile Tyr Phe 
225                 230                 235                 240 


Lys Pro Glu Tyr Lys Ile Ala Asp Pro Ile Cys Thr Phe Ile Phe Ser 
                245                 250                 255     


Ile Leu Val Leu Ala Ser Thr Ile Thr Ile Leu Lys Asp Phe Ser Ile 
            260                 265                 270         


Leu Leu Met Glu Gly Val Pro Lys Ser Leu Asn Tyr Ser Gly Val Lys 
        275                 280                 285             


Glu Leu Ile Leu Ala Val Asp Gly Val Leu Ser Val His Ser Leu His 
    290                 295                 300                 


Ile Trp Ser Leu Thr Met Asn Gln Val Ile Leu Ser Ala His Val Ala 
305                 310                 315                 320 


Thr Ala Ala Ser Arg Asp Ser Gln Val Val Arg Arg Glu Ile Ala Lys 
                325                 330                 335     


Ala Leu Ser Lys Ser Phe Thr Met His Ser Leu Thr Ile Gln Met Glu 
            340                 345                 350         


Ser Pro Val Asp Gln Asp Pro Asp Cys Leu Phe Cys Glu Asp Pro Cys 
        355                 360                 365             


Asp 
    


<210> 2
<211> 369
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 2
Met Glu Phe Leu Glu Arg Thr Tyr Leu Val Asn Asp Lys Ala Ala Lys 
1               5                   10                  15      


Met Tyr Ala Phe Thr Leu Glu Ser Val Glu Leu Gln Gln Lys Pro Val 
            20                  25                  30          


Asn Lys Asp Gln Cys Pro Arg Glu Arg Pro Glu Glu Leu Glu Ser Gly 
        35                  40                  45              


Gly Met Tyr His Cys His Ser Gly Ser Lys Pro Thr Glu Lys Gly Ala 
    50                  55                  60                  


Asn Glu Tyr Ala Tyr Ala Lys Trp Lys Leu Cys Ser Ala Ser Ala Ile 
65                  70                  75                  80  


Cys Phe Ile Phe Met Ile Ala Glu Val Val Gly Gly His Ile Ala Gly 
                85                  90                  95      


Ser Leu Ala Val Val Thr Asp Ala Ala His Leu Leu Ile Asp Leu Thr 
            100                 105                 110         


Ser Phe Leu Leu Ser Leu Phe Ser Leu Trp Leu Ser Ser Lys Pro Pro 
        115                 120                 125             


Ser Lys Arg Leu Thr Phe Gly Trp His Arg Ala Glu Ile Leu Gly Ala 
    130                 135                 140                 


Leu Leu Ser Ile Leu Cys Ile Trp Val Val Thr Gly Val Leu Val Tyr 
145                 150                 155                 160 


Leu Ala Cys Glu Arg Leu Leu Tyr Pro Asp Tyr Gln Ile Gln Ala Thr 
                165                 170                 175     


Val Met Ile Ile Val Ser Ser Cys Ala Val Ala Ala Asn Ile Val Leu 
            180                 185                 190         


Thr Val Val Leu His Gln Arg Cys Leu Gly His Asn His Lys Glu Val 
        195                 200                 205             


Gln Ala Asn Ala Ser Val Arg Ala Ala Phe Val His Ala Leu Gly Asp 
    210                 215                 220                 


Leu Phe Gln Ser Ile Ser Val Leu Ile Ser Ala Leu Ile Ile Tyr Phe 
225                 230                 235                 240 


Lys Pro Glu Tyr Lys Ile Ala Asp Pro Ile Cys Thr Phe Ile Phe Ser 
                245                 250                 255     


Ile Leu Val Leu Ala Ser Thr Ile Thr Ile Leu Lys Asp Phe Ser Ile 
            260                 265                 270         


Leu Leu Met Glu Gly Val Pro Lys Ser Leu Asn Tyr Ser Gly Val Lys 
        275                 280                 285             


Glu Leu Ile Leu Ala Val Asp Gly Val Leu Ser Val His Ser Leu His 
    290                 295                 300                 


Ile Trp Ser Leu Thr Met Asn Gln Val Ile Leu Ser Ala His Val Ala 
305                 310                 315                 320 


Thr Ala Ala Ser Trp Asp Ser Gln Val Val Arg Arg Glu Ile Ala Lys 
                325                 330                 335     


Ala Leu Ser Lys Ser Phe Thr Met His Ser Leu Thr Ile Gln Met Glu 
            340                 345                 350         


Ser Pro Val Asp Gln Asp Pro Asp Cys Leu Phe Cys Glu Asp Pro Cys 
        355                 360                 365             


Asp 
    


<210> 3
<211> 320
<212> PRT
<213> Homo sapiens

<400> 3
Met Tyr His Cys His Ser Gly Ser Lys Pro Thr Glu Lys Gly Ala Asn 
1               5                   10                  15      


Glu Tyr Ala Tyr Ala Lys Trp Lys Leu Cys Ser Ala Ser Ala Ile Cys 
            20                  25                  30          


Phe Ile Phe Met Ile Ala Glu Val Val Gly Gly His Ile Ala Gly Ser 
        35                  40                  45              


Leu Ala Val Val Thr Asp Ala Ala His Leu Leu Ile Asp Leu Thr Ser 
    50                  55                  60                  


Phe Leu Leu Ser Leu Phe Ser Leu Trp Leu Ser Ser Lys Pro Pro Ser 
65                  70                  75                  80  


Lys Arg Leu Thr Phe Gly Trp His Arg Ala Glu Ile Leu Gly Ala Leu 
                85                  90                  95      


Leu Ser Ile Leu Cys Ile Trp Val Val Thr Gly Val Leu Val Tyr Leu 
            100                 105                 110         


Ala Cys Glu Arg Leu Leu Tyr Pro Asp Tyr Gln Ile Gln Ala Thr Val 
        115                 120                 125             


Met Ile Ile Val Ser Ser Cys Ala Val Ala Ala Asn Ile Val Leu Thr 
    130                 135                 140                 


Val Val Leu His Gln Arg Cys Leu Gly His Asn His Lys Glu Val Gln 
145                 150                 155                 160 


Ala Asn Ala Ser Val Arg Ala Ala Phe Val His Ala Leu Gly Asp Leu 
                165                 170                 175     


Phe Gln Ser Ile Ser Val Leu Ile Ser Ala Leu Ile Ile Tyr Phe Lys 
            180                 185                 190         


Pro Glu Tyr Lys Ile Ala Asp Pro Ile Cys Thr Phe Ile Phe Ser Ile 
        195                 200                 205             


Leu Val Leu Ala Ser Thr Ile Thr Ile Leu Lys Asp Phe Ser Ile Leu 
    210                 215                 220                 


Leu Met Glu Gly Val Pro Lys Ser Leu Asn Tyr Ser Gly Val Lys Glu 
225                 230                 235                 240 


Leu Ile Leu Ala Val Asp Gly Val Leu Ser Val His Ser Leu His Ile 
                245                 250                 255     


Trp Ser Leu Thr Met Asn Gln Val Ile Leu Ser Ala His Val Ala Thr 
            260                 265                 270         


Ala Ala Ser Arg Asp Ser Gln Val Val Arg Arg Glu Ile Ala Lys Ala 
        275                 280                 285             


Leu Ser Lys Ser Phe Thr Met His Ser Leu Thr Ile Gln Met Glu Ser 
    290                 295                 300                 


Pro Val Asp Gln Asp Pro Asp Cys Leu Phe Cys Glu Asp Pro Cys Asp 
305                 310                 315                 320 


<210> 4
<211> 320
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 4
Met Tyr His Cys His Ser Gly Ser Lys Pro Thr Glu Lys Gly Ala Asn 
1               5                   10                  15      


Glu Tyr Ala Tyr Ala Lys Trp Lys Leu Cys Ser Ala Ser Ala Ile Cys 
            20                  25                  30          


Phe Ile Phe Met Ile Ala Glu Val Val Gly Gly His Ile Ala Gly Ser 
        35                  40                  45              


Leu Ala Val Val Thr Asp Ala Ala His Leu Leu Ile Asp Leu Thr Ser 
    50                  55                  60                  


Phe Leu Leu Ser Leu Phe Ser Leu Trp Leu Ser Ser Lys Pro Pro Ser 
65                  70                  75                  80  


Lys Arg Leu Thr Phe Gly Trp His Arg Ala Glu Ile Leu Gly Ala Leu 
                85                  90                  95      


Leu Ser Ile Leu Cys Ile Trp Val Val Thr Gly Val Leu Val Tyr Leu 
            100                 105                 110         


Ala Cys Glu Arg Leu Leu Tyr Pro Asp Tyr Gln Ile Gln Ala Thr Val 
        115                 120                 125             


Met Ile Ile Val Ser Ser Cys Ala Val Ala Ala Asn Ile Val Leu Thr 
    130                 135                 140                 


Val Val Leu His Gln Arg Cys Leu Gly His Asn His Lys Glu Val Gln 
145                 150                 155                 160 


Ala Asn Ala Ser Val Arg Ala Ala Phe Val His Ala Leu Gly Asp Leu 
                165                 170                 175     


Phe Gln Ser Ile Ser Val Leu Ile Ser Ala Leu Ile Ile Tyr Phe Lys 
            180                 185                 190         


Pro Glu Tyr Lys Ile Ala Asp Pro Ile Cys Thr Phe Ile Phe Ser Ile 
        195                 200                 205             


Leu Val Leu Ala Ser Thr Ile Thr Ile Leu Lys Asp Phe Ser Ile Leu 
    210                 215                 220                 


Leu Met Glu Gly Val Pro Lys Ser Leu Asn Tyr Ser Gly Val Lys Glu 
225                 230                 235                 240 


Leu Ile Leu Ala Val Asp Gly Val Leu Ser Val His Ser Leu His Ile 
                245                 250                 255     


Trp Ser Leu Thr Met Asn Gln Val Ile Leu Ser Ala His Val Ala Thr 
            260                 265                 270         


Ala Ala Ser Trp Asp Ser Gln Val Val Arg Arg Glu Ile Ala Lys Ala 
        275                 280                 285             


Leu Ser Lys Ser Phe Thr Met His Ser Leu Thr Ile Gln Met Glu Ser 
    290                 295                 300                 


Pro Val Asp Gln Asp Pro Asp Cys Leu Phe Cys Glu Asp Pro Cys Asp 
305                 310                 315                 320 


