                         SEQUENCE LISTING

<110>  uniQure IP B.V.
 
<120>  Improved AAV capsid production in insect cells

<130>  P6064534PCT

<150>  EP 17182429.5
<151>  2017-07-20

<160>  74    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 1

<400>  1
ctcgatgcat ggtaagcttt                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 2

<400>  2
ctgaatacat ggtcaccttt                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 3

<400>  3
ctaacttaat ggtagcattt                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 4

<400>  4
ctcaatggat ggttagtttt                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 5

<400>  5
ctcgacgtat ggtcacattt                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 6

<400>  6
ctccctgaat ggcattgttt                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 7

<400>  7
ctagcacgat ggcgtcattt                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 8

<400>  8
ctgaccgcat ggcgacgttt                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 9

<400>  9
ctggagatat ggtgagtttt                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 10

<400>  10
cttgttttat ggtaagtttt                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 11

<400>  11
ctcagttgat ggtcagcttt                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 12

<400>  12
ctacttgtat ggtagctttt                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 13

<400>  13
ctcgatgcat ggcaagcttt                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 14

<400>  14
ctgttagaat ggcgacgttt                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 15

<400>  15
ctcgaccaat gggaacgttt                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 16

<400>  16
ctggcgtcat ggggtcgttt                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 17

<400>  17
ctcgatgcat ggtaagctct                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 18

<400>  18
ctcgatgcat ggtgagcttt                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 19

<400>  19
ctcgatgcat ggtaagcctt                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 20

<400>  20
ctcctcggat ggcgtcattt                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 21

<400>  21
cttgggcgat ggtttcattt                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 22

<400>  22
ctaattgaat ggcggagttt                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 23

<400>  23
ctcgatgcat ggtaggcttt                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 24

<400>  24
ctcgatgcat ggtaagcttc                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 25

<400>  25
ctttgcttat ggtaaatttt                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 26

<400>  26
ctcgacgcat ggtaagcttt                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 27

<400>  27
ctcacttgat ggcttaattt                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 28

<400>  28
ctcagggaat gggattcttt                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 29

<400>  29
cttattctat ggtaagtttt                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence 30

<400>  30
ctcggtgcat ggtaagcttt                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence ATG2

<400>  31
ctcgatgcat ggtaagcttt                                                   20


<210>  32
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 1

<400>  32
cucgaugcau gguaagcuuu                                                   20


<210>  33
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 2

<400>  33
cugaauacau ggucaccuuu                                                   20


<210>  34
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 3

<400>  34
cuaacuuaau gguagcauuu                                                   20


<210>  35
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 4

<400>  35
cucaauggau gguuaguuuu                                                   20


<210>  36
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 5

<400>  36
cucgacguau ggucacauuu                                                   20


<210>  37
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 6

<400>  37
cucccugaau ggcauuguuu                                                   20


<210>  38
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 7

<400>  38
cuagcacgau ggcgucauuu                                                   20


<210>  39
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 8

<400>  39
cugaccgcau ggcgacguuu                                                   20


<210>  40
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 9

<400>  40
cuggagauau ggugaguuuu                                                   20


<210>  41
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 10

<400>  41
cuuguuuuau gguaaguuuu                                                   20


<210>  42
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 11

<400>  42
cucaguugau ggucagcuuu                                                   20


<210>  43
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 12

<400>  43
cuacuuguau gguagcuuuu                                                   20


<210>  44
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 13

<400>  44
cucgaugcau ggcaagcuuu                                                   20


<210>  45
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 14

<400>  45
cuguuagaau ggcgacguuu                                                   20


<210>  46
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 15

<400>  46
cucgaccaau gggaacguuu                                                   20


<210>  47
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 16

<400>  47
cuggcgucau ggggucguuu                                                   20


<210>  48
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 17

<400>  48
cucgaugcau gguaagcucu                                                   20


<210>  49
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 18

<400>  49
cucgaugcau ggugagcuuu                                                   20


<210>  50
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 19

<400>  50
cucgaugcau gguaagccuu                                                   20


<210>  51
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 20

<400>  51
cuccucggau ggcgucauuu                                                   20


<210>  52
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 21

<400>  52
cuugggcgau gguuucauuu                                                   20


<210>  53
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 22

<400>  53
cuaauugaau ggcggaguuu                                                   20


<210>  54
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 23

<400>  54
cucgaugcau gguaggcuuu                                                   20


<210>  55
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 24

<400>  55
cucgaugcau gguaagcuuc                                                   20


<210>  56
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 25

<400>  56
cuuugcuuau gguaaauuuu                                                   20


<210>  57
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 26

<400>  57
cucgacgcau gguaagcuuu                                                   20


<210>  58
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 27

<400>  58
cucacuugau ggcuuaauuu                                                   20


<210>  59
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 28

<400>  59
cucagggaau gggauucuuu                                                   20


<210>  60
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 29

<400>  60
cuuauucuau gguaaguuuu                                                   20


<210>  61
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence 30

<400>  61
cucggugcau gguaagcuuu                                                   20


<210>  62
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence derived from ATG containing sequence ATG2

<400>  62
cugucgucau ggugucguuu                                                   20


<210>  63
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTG1

<400>  63
ctcgtgccct ggcttcgttt                                                   20


<210>  64
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTG2

<400>  64
cttgatgtct ggccactttt                                                   20


<210>  65
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTG1

<400>  65
cttccactgt ggcctccttt                                                   20


<210>  66
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTG2

<400>  66
cttccgccgt ggcgtcgttt                                                   20


<210>  67
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TAG1

<400>  67
ctgcccccta ggaccgtttt                                                   20


<210>  68
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TGA1

<400>  68
cttcaccctg agcgcaattt                                                   20


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct


<220>
<221>  misc_feature
<222>  (3)..(8)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (13)..(17)
<223>  n is a, c, g, or t

<400>  69
ctnnnnnnat ggnnnnnttt                                                   20


<210>  70
<211>  2622
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAV5 expression construct sequence


<220>
<221>  promoter
<222>  (1)..(155)
<223>  Polyhedrin Promoter

<220>
<221>  misc_feature
<222>  (105)..(112)
<223>  Transcriptional start

<220>
<221>  misc_feature
<222>  (105)..(2622)
<223>  mRNA transcript

<220>
<221>  misc_feature
<222>  (156)..(158)
<223>  Translational startcodon VP1

<220>
<221>  misc_feature
<222>  (156)..(2333)
<223>  CDS ORF: AAV5 Capsid

<220>
<221>  polyA_signal
<222>  (2382)..(2622)
<223>  poly A

<400>  70
tgtaatgaga cgcacaaact aatatcacaa actggaaatg tctatcaata tatagttgct       60

gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt      120

ttcgtaacag ttttgtaata aaaaaaccta taaatatggt ctcttttgtt gatcacccac      180

ccgattggtt ggaagaagtt ggtgaaggtc ttcgcgagtt tttgggcctt gaagcgggcc      240

caccgaaacc aaaacccaat cagcagcatc aagatcaagc ccgtggtctt gtgctgcctg      300

gttataacta tctcggaccc ggaaacggtc tcgatcgagg agagcctgtc aacagggcag      360

acgaggtcgc gcgagagcac gacatctcgt acaacgagca gcttgaggcg ggagacaacc      420

cctacctcaa gtacaaccac gcggacgccg agtttcagga gaagctcgcc gacgacacat      480

ccttcggggg aaacctcgga aaggcagtct ttcaggccaa gaaaagggtt ctcgaacctt      540

ttggcctggt tgaagagggt gctaagacgg cccctaccgg aaagcggata gacgaccact      600

ttccaaaaag aaagaaggct cggaccgaag aggactccaa gccttccacc tcgtcagacg      660

ccgaagctgg acccagcgga tcccagcagc tgcaaatccc agcccaacca gcctcaagtt      720

tgggagctga tacaatgtct gcgggaggtg gcggcccatt gggcgacaat aaccaaggtg      780

ccgatggagt gggcaatgcc tcgggagatt ggcattgcga ttccacgtgg atgggggaca      840

gagtcgtcac caagtccacc cgaacctggg tgctgcccag ctacaacaac caccagtacc      900

gagagatcaa aagcggctcc gtcgacggaa gcaacgccaa cgcctacttt ggatacagca      960

ccccctgggg gtactttgac tttaaccgct tccacagcca ctggagcccc cgagactggc     1020

aaagactcat caacaactac tggggcttca gaccccggtc cctcagagtc aaaatcttca     1080

acattcaagt caaagaggtc acggtgcagg actccaccac caccatcgcc aacaacctca     1140

cctccaccgt ccaagtgttt acggacgacg actaccagct gccctacgtc gtcggcaacg     1200

ggaccgaggg atgcctgccg gccttccctc cgcaggtctt tacgctgccg cagtacggtt     1260

acgcgacgct gaaccgcgac aacacagaaa atcccaccga gaggagcagc ttcttctgcc     1320

tagagtactt tcccagcaag atgctgagaa cgggcaacaa ctttgagttt acctacaact     1380

ttgaggaggt gcccttccac tccagcttcg ctcccagtca gaacctgttc aagctggcca     1440

acccgctggt ggaccagtac ttgtaccgct tcgtgagcac aaataacact ggcggagtcc     1500

agttcaacaa gaacctggcc gggagatacg ccaacaccta caaaaactgg ttcccggggc     1560

ccatgggccg aacccagggc tggaacctgg gctccggggt caaccgcgcc agtgtcagcg     1620

ccttcgccac gaccaatagg atggagctcg agggcgcgag ttaccaggtg cccccgcagc     1680

cgaacggcat gaccaacaac ctccagggca gcaacaccta tgccctggag aacactatga     1740

tcttcaacag ccagccggcg aacccgggca ccaccgccac gtacctcgag ggcaacatgc     1800

tcatcaccag cgagagcgag acgcagccgg tgaaccgcgt ggcgtacaac gtcggcgggc     1860

agatggccac caacaaccag agctccacca ctgcccccgc gaccggcacg tacaacctcc     1920

aggaaatcgt gcccggcagc gtgtggatgg agagggacgt gtacctccaa ggacccatct     1980

gggccaagat cccagagacg ggggcgcact ttcacccctc tccggccatg ggcggattcg     2040

gactcaaaca cccaccgccc atgatgctca tcaagaacac gcctgtgccc ggaaatatca     2100

ccagcttctc ggacgtgccc gtcagcagct tcatcaccca gtacagcacc gggcaggtca     2160

ccgtggagat ggagtgggag ctcaagaagg aaaactccaa gaggtggaac ccagagatcc     2220

agtacacaaa caactacaac gacccccagt ttgtggactt tgccccggac agcaccgggg     2280

aatacagaac caccagacct atcggaaccc gataccttac ccgacccctt taatctagag     2340

cctgcagtct cgacaagcta gcttgtcgag aagtactaga ggatcataat cagccatacc     2400

acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa     2460

cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa     2520

taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt     2580

ggtttgtcca aactcatcaa tgtatcttat catgtctgga tc                        2622


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct


<220>
<221>  misc_feature
<222>  (3)..(11)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  N is part of a start codon and can be any of a, c, t, or g

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  N is part of a start codon and can be any of t, g, or a

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  N is part of a start codon and can be any of a, c, t, or g

<220>
<221>  misc_feature
<222>  (13)..(17)
<223>  n is a, c, g, or t

<400>  71
ctnnnnnnnn ngnnnnnttt                                                   20


<210>  72
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence 1

<400>  72

Met His His Gly Lys Leu 
1               5       


<210>  73
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence 2

<400>  73

Met Glu Ile Trp 
1               


<210>  74
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG containing sequence derived from SEQ ID NO:70

<400>  74
ctataaatat ggtctctttt                                                   20


