                         SEQUENCE LISTING

<110>  METABOLIC EXPLORER
 
<120>  Microorganism and method for improved 1,3-propanediol production 
       by fermentation on a culture medium with high glycerine content

<130>  B374950PCT D37437

<150>  EP17306044.3
<151>  2017-08-04

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  690
<212>  DNA
<213>  Clostridium acetobutylicum


<220>
<221>  misc_feature
<223>  CA_C0884 gene sequence

<400>  1
atggataaaa aaattttaac cgtattaaaa cattgtatct tattttcaaa aattgaaact       60

caggaaattg atcatttatt ttcatctata aattactcaa taaaagaata ctataaagat      120

gaaactatcg caattgaagg cgatacgtgc aataaaattg gaatagtatt aagcggttgt      180

gttgaaatac agaaaattta cgaatctggt aaaagtctta caataacaac actagaagaa      240

agcaagatat ttggtgaagc aataatattc tccaataaaa caagctatcc ttctacaata      300

atagcatgta ctaaaagtac tataattttt attcccaaag cctcaatatc taaattatgc      360

agcgacaatt cccttttcct taatggcttt atgtctcttc tatctaataa gatattgatg      420

ttgaataaaa aattaaaaaa tatgtcttat catactataa gagagaaaat atcaaactat      480

atacttgaac agtacgaagc tcaaaataac ttaactttta aaatgaataa gtcaaaaaaa      540

cagttgtctg aaatgcttgg aattccaagg ccatcattat ctagagaatt cataaaccta      600

cgtgaagaag gaattataga ctttgataga acatctatta caatattaga tattaactct      660

ttaaaagaaa ttttagaagc tgcagaataa                                       690


<210>  2
<211>  229
<212>  PRT
<213>  Clostridium acetobutylicum


<220>
<221>  MISC_FEATURE
<223>  CA_C0884 protein sequence : NP_347520.1

<400>  2

Met Asp Lys Lys Ile Leu Thr Val Leu Lys His Cys Ile Leu Phe Ser 
1               5                   10                  15      


Lys Ile Glu Thr Gln Glu Ile Asp His Leu Phe Ser Ser Ile Asn Tyr 
            20                  25                  30          


Ser Ile Lys Glu Tyr Tyr Lys Asp Glu Thr Ile Ala Ile Glu Gly Asp 
        35                  40                  45              


Thr Cys Asn Lys Ile Gly Ile Val Leu Ser Gly Cys Val Glu Ile Gln 
    50                  55                  60                  


Lys Ile Tyr Glu Ser Gly Lys Ser Leu Thr Ile Thr Thr Leu Glu Glu 
65                  70                  75                  80  


Ser Lys Ile Phe Gly Glu Ala Ile Ile Phe Ser Asn Lys Thr Ser Tyr 
                85                  90                  95      


Pro Ser Thr Ile Ile Ala Cys Thr Lys Ser Thr Ile Ile Phe Ile Pro 
            100                 105                 110         


Lys Ala Ser Ile Ser Lys Leu Cys Ser Asp Asn Ser Leu Phe Leu Asn 
        115                 120                 125             


Gly Phe Met Ser Leu Leu Ser Asn Lys Ile Leu Met Leu Asn Lys Lys 
    130                 135                 140                 


Leu Lys Asn Met Ser Tyr His Thr Ile Arg Glu Lys Ile Ser Asn Tyr 
145                 150                 155                 160 


Ile Leu Glu Gln Tyr Glu Ala Gln Asn Asn Leu Thr Phe Lys Met Asn 
                165                 170                 175     


Lys Ser Lys Lys Gln Leu Ser Glu Met Leu Gly Ile Pro Arg Pro Ser 
            180                 185                 190         


Leu Ser Arg Glu Phe Ile Asn Leu Arg Glu Glu Gly Ile Ile Asp Phe 
        195                 200                 205             


Asp Arg Thr Ser Ile Thr Ile Leu Asp Ile Asn Ser Leu Lys Glu Ile 
    210                 215                 220                 


Leu Glu Ala Ala Glu 
225                 


<210>  3
<211>  711
<212>  DNA
<213>  Clostridium acetobutylicum


<220>
<221>  misc_feature
<223>  CA_C0885 gene sequence

<400>  3
atgaaaagaa aaatagtaaa catagataaa gataaatgta atggatgtgg actttgtagt       60

gaagcatgtc atgaaaatgc aattgaaata attaatggaa aagcagagct tttatctgat      120

gaatattgtg atggtttagg agattgttta cctcattgtc cagttgatgc aataactata      180

atagagagag aaagtaagga atatgatgaa gaggcagttc agagaagaat tgaagaaaag      240

aaaaaatcaa agttagctaa accctgtgga tgtccaggag ctatggctaa aaaaatagaa      300

agagtagcta agcctttagc taaagtaaag gaagataggt cttctgtttc agagttaatg      360

cagtggccag ttcagttaag gcttgtaagt ccaggagctc catattttaa aaatgctaat      420

cttttagtag ctgcagattg tacagcctat gcttatggtg actttcacaa tgattttata      480

aagaatcata taacagtaat aggatgtcca aaacttgatg atgttacata ttacaaagat      540

aagttgaaag aaattataga acttaatgac cttaagagta taacagttgt tagaatggag      600

gtaccttgct gttcaggcat agtttcagca gtaaagactg ctatgcttga agcaaaagtt      660

atagtacctt ttagagaagt tattatagga actaatggtg aaattagata a               711


<210>  4
<211>  236
<212>  PRT
<213>  Clostridium acetobutylicum


<220>
<221>  MISC_FEATURE
<223>  CA_C0885 protein sequence : NP_347521.1

<400>  4

Met Lys Arg Lys Ile Val Asn Ile Asp Lys Asp Lys Cys Asn Gly Cys 
1               5                   10                  15      


Gly Leu Cys Ser Glu Ala Cys His Glu Asn Ala Ile Glu Ile Ile Asn 
            20                  25                  30          


Gly Lys Ala Glu Leu Leu Ser Asp Glu Tyr Cys Asp Gly Leu Gly Asp 
        35                  40                  45              


Cys Leu Pro His Cys Pro Val Asp Ala Ile Thr Ile Ile Glu Arg Glu 
    50                  55                  60                  


Ser Lys Glu Tyr Asp Glu Glu Ala Val Gln Arg Arg Ile Glu Glu Lys 
65                  70                  75                  80  


Lys Lys Ser Lys Leu Ala Lys Pro Cys Gly Cys Pro Gly Ala Met Ala 
                85                  90                  95      


Lys Lys Ile Glu Arg Val Ala Lys Pro Leu Ala Lys Val Lys Glu Asp 
            100                 105                 110         


Arg Ser Ser Val Ser Glu Leu Met Gln Trp Pro Val Gln Leu Arg Leu 
        115                 120                 125             


Val Ser Pro Gly Ala Pro Tyr Phe Lys Asn Ala Asn Leu Leu Val Ala 
    130                 135                 140                 


Ala Asp Cys Thr Ala Tyr Ala Tyr Gly Asp Phe His Asn Asp Phe Ile 
145                 150                 155                 160 


Lys Asn His Ile Thr Val Ile Gly Cys Pro Lys Leu Asp Asp Val Thr 
                165                 170                 175     


Tyr Tyr Lys Asp Lys Leu Lys Glu Ile Ile Glu Leu Asn Asp Leu Lys 
            180                 185                 190         


Ser Ile Thr Val Val Arg Met Glu Val Pro Cys Cys Ser Gly Ile Val 
        195                 200                 205             


Ser Ala Val Lys Thr Ala Met Leu Glu Ala Lys Val Ile Val Pro Phe 
    210                 215                 220                 


Arg Glu Val Ile Ile Gly Thr Asn Gly Glu Ile Arg 
225                 230                 235     


<210>  5
<211>  684
<212>  DNA
<213>  Clostridium pasteurianum


<220>
<221>  misc_feature
<223>  BEE63_RS05095 gene sequence

<400>  5
atggataaag aacttttaag tatattaaag aactgtattt tattttgcaa aattgatgat       60

actaaaatta atgagctttt ttcatatata aattattcaa taaaaaaata ctcaaatgga      120

gaaactgtgg ccattgaagg tgacgagtgc agcagcatag gtataatttt agacggtttt      180

gctgagatac aaaaaatcta cgaatccggc aaaagtttaa ctatagagac cttaaacact      240

aacaagatat ttggtgaagc aataatattt tctaaaaaaa atacttatcc ggctacaata      300

atttcatgtg ccaaaacaag tatattattc ataccaaaat cctcaataat taaattatgc      360

agcgaaaatt caaattttct aaacggcttt atgtctcttc tatctaataa aatattaatg      420

ctaaataaaa agttaaaaaa tctttcatat aacacaatta gagaaaaagt agcaaattac      480

ctacttgaag agactataat ccaaaaaaat ccaaatataa aaatgaataa atcaaaaaaa      540

cagctgtctg aattactcgg tattccacgt ccttctctgt ctagagaatt aataaagtta      600

aggaacgatg gtatcatttc ctttgataga aattatatta aaatattaaa tatggatgag      660

ttaaaaaata tcctagataa gtag                                             684


<210>  6
<211>  227
<212>  PRT
<213>  Clostridium pasteurianum


<220>
<221>  MISC_FEATURE
<223>  BEE63_RS05095 protein sequence : WP_066020350

<400>  6

Met Asp Lys Glu Leu Leu Ser Ile Leu Lys Asn Cys Ile Leu Phe Cys 
1               5                   10                  15      


Lys Ile Asp Asp Thr Lys Ile Asn Glu Leu Phe Ser Tyr Ile Asn Tyr 
            20                  25                  30          


Ser Ile Lys Lys Tyr Ser Asn Gly Glu Thr Val Ala Ile Glu Gly Asp 
        35                  40                  45              


Glu Cys Ser Ser Ile Gly Ile Ile Leu Asp Gly Phe Ala Glu Ile Gln 
    50                  55                  60                  


Lys Ile Tyr Glu Ser Gly Lys Ser Leu Thr Ile Glu Thr Leu Asn Thr 
65                  70                  75                  80  


Asn Lys Ile Phe Gly Glu Ala Ile Ile Phe Ser Lys Lys Asn Thr Tyr 
                85                  90                  95      


Pro Ala Thr Ile Ile Ser Cys Ala Lys Thr Ser Ile Leu Phe Ile Pro 
            100                 105                 110         


Lys Ser Ser Ile Ile Lys Leu Cys Ser Glu Asn Ser Asn Phe Leu Asn 
        115                 120                 125             


Gly Phe Met Ser Leu Leu Ser Asn Lys Ile Leu Met Leu Asn Lys Lys 
    130                 135                 140                 


Leu Lys Asn Leu Ser Tyr Asn Thr Ile Arg Glu Lys Val Ala Asn Tyr 
145                 150                 155                 160 


Leu Leu Glu Glu Thr Ile Ile Gln Lys Asn Pro Asn Ile Lys Met Asn 
                165                 170                 175     


Lys Ser Lys Lys Gln Leu Ser Glu Leu Leu Gly Ile Pro Arg Pro Ser 
            180                 185                 190         


Leu Ser Arg Glu Leu Ile Lys Leu Arg Asn Asp Gly Ile Ile Ser Phe 
        195                 200                 205             


Asp Arg Asn Tyr Ile Lys Ile Leu Asn Met Asp Glu Leu Lys Asn Ile 
    210                 215                 220                 


Leu Asp Lys 
225         


<210>  7
<211>  690
<212>  DNA
<213>  Clostridium butyricum


<220>
<221>  misc_feature
<223>  CBY_RS14495 gene sequence

<400>  7
atgaaaaatg ttaatgagat tatagataaa ataaagcaaa atgaattttt taaggggata       60

gatgataaaa aaatagaaat gattatttca gaattaagtc atatttctaa ggaatattca      120

aaaggacagg taattgctaa tgagggggag gtttgtaaaa atctaggatt agtcgtagat      180

ggaattgttg agatacagag aatatactca agcggaaaac atatagttct taaacgtatg      240

ggagcaggag aagtttttgg agaagccata atattttcag ataaaaataa atatccagcc      300

acaataattg cttcttcgga ttgtataata tcttatttga aaaaagaaga tattattaaa      360

ctttgcctta atgaagaaat aatcttaaag aattttataa ctttattgag caataaaatt      420

tttatattaa atagaaaaat aaaaaccata tcttttaaaa caatacgaca gaaagttgtt      480

aactttatat tagaacagtc aaaaagtcag aacaataaaa ctgtaatttt gaaaataagt      540

aaagagcaga tagcatcttt acttggaata cctagaccat cactttcaag agagcttatg      600

aagcttagag atgatggact tattgaattt gataggaaca aaataagtat tataaatata      660

gaaagacttg aagaagaact tttagaatag                                       690


<210>  8
<211>  229
<212>  PRT
<213>  Clostridium butyricum


<220>
<221>  MISC_FEATURE
<223>  CBY_RS14495 protein sequence : WP_003410673.1

<400>  8

Met Lys Asn Val Asn Glu Ile Ile Asp Lys Ile Lys Gln Asn Glu Phe 
1               5                   10                  15      


Phe Lys Gly Ile Asp Asp Lys Lys Ile Glu Met Ile Ile Ser Glu Leu 
            20                  25                  30          


Ser His Ile Ser Lys Glu Tyr Ser Lys Gly Gln Val Ile Ala Asn Glu 
        35                  40                  45              


Gly Glu Val Cys Lys Asn Leu Gly Leu Val Val Asp Gly Ile Val Glu 
    50                  55                  60                  


Ile Gln Arg Ile Tyr Ser Ser Gly Lys His Ile Val Leu Lys Arg Met 
65                  70                  75                  80  


Gly Ala Gly Glu Val Phe Gly Glu Ala Ile Ile Phe Ser Asp Lys Asn 
                85                  90                  95      


Lys Tyr Pro Ala Thr Ile Ile Ala Ser Ser Asp Cys Ile Ile Ser Tyr 
            100                 105                 110         


Leu Lys Lys Glu Asp Ile Ile Lys Leu Cys Leu Asn Glu Glu Ile Ile 
        115                 120                 125             


Leu Lys Asn Phe Ile Thr Leu Leu Ser Asn Lys Ile Phe Ile Leu Asn 
    130                 135                 140                 


Arg Lys Ile Lys Thr Ile Ser Phe Lys Thr Ile Arg Gln Lys Val Val 
145                 150                 155                 160 


Asn Phe Ile Leu Glu Gln Ser Lys Ser Gln Asn Asn Lys Thr Val Ile 
                165                 170                 175     


Leu Lys Ile Ser Lys Glu Gln Ile Ala Ser Leu Leu Gly Ile Pro Arg 
            180                 185                 190         


Pro Ser Leu Ser Arg Glu Leu Met Lys Leu Arg Asp Asp Gly Leu Ile 
        195                 200                 205             


Glu Phe Asp Arg Asn Lys Ile Ser Ile Ile Asn Ile Glu Arg Leu Glu 
    210                 215                 220                 


Glu Glu Leu Leu Glu 
225                 


<210>  9
<211>  690
<212>  DNA
<213>  Clostridium butyricum


<220>
<221>  misc_feature
<223>  K670_RS19640 gene sequence

<400>  9
atgaaaaatg ctaatgagat tatagataaa ataaagcaaa atgaattttt taaggggata       60

gatgataaaa aaatagaaat gattatttca gaattaagtc atatttctaa ggaatattca      120

aaaggacagg taattgctaa tgagggggag gtttgtaaaa atctaggatt agtcgtagat      180

ggaattgttg agatacagag aatatactca agcggaaaac atatagttct taaacgtatg      240

ggagcaggag aagtttttgg agaagccata atattttcag ataaaaataa atatccagcc      300

acaataattg cttcttcgga ttgtataata tcttatttga aaaaagaaga tattattaaa      360

ctttgcctta atgaagaaat aattttaaag aattttataa ctttattgag caataaaatt      420

tttatattaa atagaaaaat aaaaaccata tcttttaaaa caatacgaca gaaagttgtt      480

aactttatat tagaacagtc aaaaagtcag aacaataaaa ctgtaatttt gaaaataagt      540

aaagagcaga tagcatcttt acttggaata cctagaccat cactttcaag agagcttatg      600

aaacttagag atgatggact tattgaattt gataggaaca aaataagtat tataaatata      660

gaaagacttg aagaagaact tttagaatag                                       690


<210>  10
<211>  229
<212>  PRT
<213>  Clostridium butyricum


<220>
<221>  MISC_FEATURE
<223>  K670_RS19640 protein sequence : WP_035765453.1

<400>  10

Met Lys Asn Ala Asn Glu Ile Ile Asp Lys Ile Lys Gln Asn Glu Phe 
1               5                   10                  15      


Phe Lys Gly Ile Asp Asp Lys Lys Ile Glu Met Ile Ile Ser Glu Leu 
            20                  25                  30          


Ser His Ile Ser Lys Glu Tyr Ser Lys Gly Gln Val Ile Ala Asn Glu 
        35                  40                  45              


Gly Glu Val Cys Lys Asn Leu Gly Leu Val Val Asp Gly Ile Val Glu 
    50                  55                  60                  


Ile Gln Arg Ile Tyr Ser Ser Gly Lys His Ile Val Leu Lys Arg Met 
65                  70                  75                  80  


Gly Ala Gly Glu Val Phe Gly Glu Ala Ile Ile Phe Ser Asp Lys Asn 
                85                  90                  95      


Lys Tyr Pro Ala Thr Ile Ile Ala Ser Ser Asp Cys Ile Ile Ser Tyr 
            100                 105                 110         


Leu Lys Lys Glu Asp Ile Ile Lys Leu Cys Leu Asn Glu Glu Ile Ile 
        115                 120                 125             


Leu Lys Asn Phe Ile Thr Leu Leu Ser Asn Lys Ile Phe Ile Leu Asn 
    130                 135                 140                 


Arg Lys Ile Lys Thr Ile Ser Phe Lys Thr Ile Arg Gln Lys Val Val 
145                 150                 155                 160 


Asn Phe Ile Leu Glu Gln Ser Lys Ser Gln Asn Asn Lys Thr Val Ile 
                165                 170                 175     


Leu Lys Ile Ser Lys Glu Gln Ile Ala Ser Leu Leu Gly Ile Pro Arg 
            180                 185                 190         


Pro Ser Leu Ser Arg Glu Leu Met Lys Leu Arg Asp Asp Gly Leu Ile 
        195                 200                 205             


Glu Phe Asp Arg Asn Lys Ile Ser Ile Ile Asn Ile Glu Arg Leu Glu 
    210                 215                 220                 


Glu Glu Leu Leu Glu 
225                 


<210>  11
<211>  690
<212>  DNA
<213>  Clostridium butyricum


<220>
<221>  misc_feature
<223>  AT697_RS15885 gene sequence

<400>  11
atgaaaaatg ttaatgagat tatagataaa ataaagcaaa atgaattttt taaggggata       60

gatgataaaa aaatagaaat gattatttca gaattaagtc atatttataa ggaatattca      120

aaaggacagg taattgctaa tgagggggag gtttgtaaaa atctaggatt agtcgtagat      180

ggaattgttg agatacagag aatatactca agcggaaaac atatagttct taaacgtatg      240

ggagcaggag aagtttttgg agaagccata atattttcag ataaaaataa atatccagcc      300

acaataattg cttcttcgga ttgtataata tcttatttga aaaaagaaga tattattaaa      360

ctttgcctta atgaagaaat aatcttaaag aattttataa ctttattgag caataaaatt      420

tttatattaa atagaaaaat aaaaaccata tcttttaaaa caatacgaca gaaagttgtt      480

aactttatat tagaacagtc aaaaagtcag aacaataaaa ctgtaatttt gaaaataagt      540

aaagagcaga tagcatcttt acttggaata cctagaccat cactttcaag agagcttatg      600

aagcttagag atgatggact tattgaattt gataggaaca aaataagtat tataaatata      660

gaaagacttg aagaagagct tttagaatag                                       690


<210>  12
<211>  229
<212>  PRT
<213>  Clostridium butyricum


<220>
<221>  MISC_FEATURE
<223>  AT697_RS15885 protein sequence : WP_046057470.1

<400>  12

Met Lys Asn Val Asn Glu Ile Ile Asp Lys Ile Lys Gln Asn Glu Phe 
1               5                   10                  15      


Phe Lys Gly Ile Asp Asp Lys Lys Ile Glu Met Ile Ile Ser Glu Leu 
            20                  25                  30          


Ser His Ile Tyr Lys Glu Tyr Ser Lys Gly Gln Val Ile Ala Asn Glu 
        35                  40                  45              


Gly Glu Val Cys Lys Asn Leu Gly Leu Val Val Asp Gly Ile Val Glu 
    50                  55                  60                  


Ile Gln Arg Ile Tyr Ser Ser Gly Lys His Ile Val Leu Lys Arg Met 
65                  70                  75                  80  


Gly Ala Gly Glu Val Phe Gly Glu Ala Ile Ile Phe Ser Asp Lys Asn 
                85                  90                  95      


Lys Tyr Pro Ala Thr Ile Ile Ala Ser Ser Asp Cys Ile Ile Ser Tyr 
            100                 105                 110         


Leu Lys Lys Glu Asp Ile Ile Lys Leu Cys Leu Asn Glu Glu Ile Ile 
        115                 120                 125             


Leu Lys Asn Phe Ile Thr Leu Leu Ser Asn Lys Ile Phe Ile Leu Asn 
    130                 135                 140                 


Arg Lys Ile Lys Thr Ile Ser Phe Lys Thr Ile Arg Gln Lys Val Val 
145                 150                 155                 160 


Asn Phe Ile Leu Glu Gln Ser Lys Ser Gln Asn Asn Lys Thr Val Ile 
                165                 170                 175     


Leu Lys Ile Ser Lys Glu Gln Ile Ala Ser Leu Leu Gly Ile Pro Arg 
            180                 185                 190         


Pro Ser Leu Ser Arg Glu Leu Met Lys Leu Arg Asp Asp Gly Leu Ile 
        195                 200                 205             


Glu Phe Asp Arg Asn Lys Ile Ser Ile Ile Asn Ile Glu Arg Leu Glu 
    210                 215                 220                 


Glu Glu Leu Leu Glu 
225                 


<210>  13
<211>  711
<212>  DNA
<213>  Clostridium pasteurianum


<220>
<221>  misc_feature
<223>  BEE63_RS05090 gene sequence

<400>  13
atgaaaagaa aaatagtgaa tatagataaa gaaaaatgta atggatgtgg actttgtagt       60

aaggcatgcc atgaaaatgc tatagaaata attgatggaa aagcagaact tgtttcggat      120

gaatattgtg atggacttgg agattgcctt ccacattgtc cagaaaacgc tataactata      180

atagaaagag aaagtaaaga atacgatgaa gaagctgtaa aaagaagaat tgaaagtaaa      240

gaaaagaaaa tgccatgtgg atgtccaggt tcaatggcaa aaaagataac tagagtttct      300

aaaaaagtag aagttaaaca tgaagatacg ggtgcagcat cagaacttat gcagtggcct      360

gtacaattgc aattagtaaa tccaaatgca agctattttg aaggcgcaaa tcttttagtt      420

gcagcggatt gtacagctta tgcttatggt aactttcatc aagattttat aaaaaatcat      480

ataactgtaa taggttgtcc taaacttgac gatgttgagt actataagaa taaaatcaaa      540

gaaattatag aaaataataa tcttaaaagt attacagtta ccagaatgga agtaccatgc      600

tgtggcggta tagtgtcagc tgtaaagaat gctatgcttg aggcacaggt tatattacct      660

tatagagaag tagttatagg aactaatgga gaagtgaagg aagcgaagta a               711


<210>  14
<211>  236
<212>  PRT
<213>  Clostridium pasteurianum


<220>
<221>  MISC_FEATURE
<223>  BEE63_RS05090 protein sequence : WP_066020349.1

<400>  14

Met Lys Arg Lys Ile Val Asn Ile Asp Lys Glu Lys Cys Asn Gly Cys 
1               5                   10                  15      


Gly Leu Cys Ser Lys Ala Cys His Glu Asn Ala Ile Glu Ile Ile Asp 
            20                  25                  30          


Gly Lys Ala Glu Leu Val Ser Asp Glu Tyr Cys Asp Gly Leu Gly Asp 
        35                  40                  45              


Cys Leu Pro His Cys Pro Glu Asn Ala Ile Thr Ile Ile Glu Arg Glu 
    50                  55                  60                  


Ser Lys Glu Tyr Asp Glu Glu Ala Val Lys Arg Arg Ile Glu Ser Lys 
65                  70                  75                  80  


Glu Lys Lys Met Pro Cys Gly Cys Pro Gly Ser Met Ala Lys Lys Ile 
                85                  90                  95      


Thr Arg Val Ser Lys Lys Val Glu Val Lys His Glu Asp Thr Gly Ala 
            100                 105                 110         


Ala Ser Glu Leu Met Gln Trp Pro Val Gln Leu Gln Leu Val Asn Pro 
        115                 120                 125             


Asn Ala Ser Tyr Phe Glu Gly Ala Asn Leu Leu Val Ala Ala Asp Cys 
    130                 135                 140                 


Thr Ala Tyr Ala Tyr Gly Asn Phe His Gln Asp Phe Ile Lys Asn His 
145                 150                 155                 160 


Ile Thr Val Ile Gly Cys Pro Lys Leu Asp Asp Val Glu Tyr Tyr Lys 
                165                 170                 175     


Asn Lys Ile Lys Glu Ile Ile Glu Asn Asn Asn Leu Lys Ser Ile Thr 
            180                 185                 190         


Val Thr Arg Met Glu Val Pro Cys Cys Gly Gly Ile Val Ser Ala Val 
        195                 200                 205             


Lys Asn Ala Met Leu Glu Ala Gln Val Ile Leu Pro Tyr Arg Glu Val 
    210                 215                 220                 


Val Ile Gly Thr Asn Gly Glu Val Lys Glu Ala Lys 
225                 230                 235     


<210>  15
<211>  1524
<212>  DNA
<213>  Clostridium acetobutylicum


<220>
<221>  misc_feature
<223>  Full sequence comprising CA_C0884, the intergenic region, and 
       CA_C0885

<400>  15
ttattctgca gcttctaaaa tttcttttaa agagttaata tctaatattg taatagatgt       60

tctatcaaag tctataattc cttcttcacg taggtttatg aattctctag ataatgatgg      120

ccttggaatt ccaagcattt cagacaactg tttttttgac ttattcattt taaaagttaa      180

gttattttga gcttcgtact gttcaagtat atagtttgat attttctctc ttatagtatg      240

ataagacata ttttttaatt ttttattcaa catcaatatc ttattagata gaagagacat      300

aaagccatta aggaaaaggg aattgtcgct gcataattta gatattgagg ctttgggaat      360

aaaaattata gtacttttag tacatgctat tattgtagaa ggatagcttg ttttattgga      420

gaatattatt gcttcaccaa atatcttgct ttcttctagt gttgttattg taagactttt      480

accagattcg taaattttct gtatttcaac acaaccgctt aatactattc caattttatt      540

gcacgtatcg ccttcaattg cgatagtttc atctttatag tattctttta ttgagtaatt      600

tatagatgaa aataaatgat caatttcctg agtttcaatt tttgaaaata agatacaatg      660

ttttaatacg gttaaaattt ttttatccat gtttttctcc caatactttt ttgtgtaaca      720

tatgttactg atatatttat ataattttaa tataatttaa tcataaagac aagtaatttt      780

attttgcgga actttatatt taggaggaaa aaaatgaaaa gaaaaatagt aaacatagat      840

aaagataaat gtaatggatg tggactttgt agtgaagcat gtcatgaaaa tgcaattgaa      900

ataattaatg gaaaagcaga gcttttatct gatgaatatt gtgatggttt aggagattgt      960

ttacctcatt gtccagttga tgcaataact ataatagaga gagaaagtaa ggaatatgat     1020

gaagaggcag ttcagagaag aattgaagaa aagaaaaaat caaagttagc taaaccctgt     1080

ggatgtccag gagctatggc taaaaaaata gaaagagtag ctaagccttt agctaaagta     1140

aaggaagata ggtcttctgt ttcagagtta atgcagtggc cagttcagtt aaggcttgta     1200

agtccaggag ctccatattt taaaaatgct aatcttttag tagctgcaga ttgtacagcc     1260

tatgcttatg gtgactttca caatgatttt ataaagaatc atataacagt aataggatgt     1320

ccaaaacttg atgatgttac atattacaaa gataagttga aagaaattat agaacttaat     1380

gaccttaaga gtataacagt tgttagaatg gaggtacctt gctgttcagg catagtttca     1440

gcagtaaaga ctgctatgct tgaagcaaaa gttatagtac cttttagaga agttattata     1500

ggaactaatg gtgaaattag ataa                                            1524


<210>  16
<211>  123
<212>  DNA
<213>  Clostridium acetobutylicum


<220>
<221>  misc_feature
<223>  Intergenic region between positions 1014117 and 1014239 of the C.
       acetobutylicum ATCC 824 genome

<400>  16
gtttttctcc caatactttt ttgtgtaaca tatgttactg atatatttat ataattttaa       60

tataatttaa tcataaagac aagtaatttt attttgcgga actttatatt taggaggaaa      120

aaa                                                                    123


<210>  17
<211>  124
<212>  DNA
<213>  Artificial

<220>
<223>  Intergenic region comprising a single nucleotide insertion

<400>  17
gtttttctcc caatactttt ttgtgtaaca tatgttactg atatatttat ataattttaa       60

tataatttaa tcataaagac aagtaatttt attttgcgga actttatatt taggaggaaa      120

aaaa                                                                   124


<210>  18
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  Intergenic region forward primer

<400>  18
gccattaagg aaaaggg                                                      17


<210>  19
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Intergenic region reverse primer

<400>  19
gctcctggac ttacaagcc                                                    19


<210>  20
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  CA_C0884 gene based forward primer

<400>  20
gaagagacat aaagccatta                                                   20


<210>  21
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  CA_C0884 gene based reverse primer

<400>  21
acaataacaa cactagaaga a                                                 21


<210>  22
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  CA_C0885 gene based forward primer

<400>  22
gctaaaccct gtggatgtc                                                    19


<210>  23
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  CA_C0885 gene based reverse primer

<400>  23
ggacttacaa gccttaactg aa                                                22


<210>  24
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  CA_C1628 gene based forward primer

<400>  24
cccttagagg aggctatg                                                     18


<210>  25
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  CA_C1628 gene based reverse primer

<400>  25
gccatctctt acatctgg                                                     18


