                         SEQUENCE LISTING

<110>  Lentigen Technology, Inc.
 
<120>  COMPOSITIONS AND METHODS FOR TREATING CANCER WITH ANTI-CD19/CD20 
       IMMUNOTHERAPY

<130>  42449-0015WO1

<150>  US 62/539,483
<151>  2017-07-31

<160>  49    

<170>  PatentIn version 3.5

<210>  1
<211>  2283
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Leader-CD19 VL-Whitlow linker CD19 VH (GGGGS)-5 CD20 VH (GGGGS)-3
       CD20 VL CD8 hinge+TM-4-1BB- CD3z  (Construct CAR 1920)

<400>  1
atgctccttc tcgtgacctc cctgcttctc tgcgaactgc cccatcctgc cttcctgctg       60

attcccgaca ttcagatgac tcagaccacc tcctccctgt ccgcctccct gggcgaccgc      120

gtgaccatct catgccgcgc cagccaggac atctcgaagt acctcaactg gtaccagcag      180

aagcccgacg gaaccgtgaa gctcctgatc taccacacct cccggctgca cagcggagtg      240

ccgtctagat tctcgggttc ggggtcggga actgactact cccttactat ttccaacctg      300

gagcaggagg atattgccac ctacttctgc caacaaggaa acaccctgcc gtacactttt      360

ggcgggggaa ccaagctgga aatcactggc agcacatccg gttccgggaa gcccggctcc      420

ggagagggca gcaccaaggg ggaagtcaag ctgcaggaat caggacctgg cctggtggcc      480

ccgagccagt cactgtccgt gacttgtact gtgtccggag tgtcgctccc ggattacgga      540

gtgtcctgga tcaggcagcc acctcggaaa ggattggaat ggctcggagt catctggggt      600

tccgaaacca cctattacaa ctcggcactg aaatccaggc tcaccattat caaggataac      660

tccaagtcac aagtgttcct gaagatgaat agcctgcaga ctgacgacac ggcgatctac      720

tattgcgcca agcactacta ctacggcgga tcctacgcta tggactactg gggccagggg      780

accagcgtga ccgtgtcatc cggaggcggc ggcagcggcg ggggagggtc cggagggggt      840

ggttctggtg gaggaggatc gggaggcggt ggcagcgagg tgcagttgca acagtcagga      900

gctgaactgg tcaagccagg agccagcgtg aagatgagct gcaaggcctc cggttacacc      960

ttcacctcct acaacatgca ctgggtgaaa cagaccccgg gacaagggct cgaatggatt     1020

ggcgccatct accccgggaa tggcgatact tcgtacaacc agaagttcaa gggaaaggcc     1080

accctgaccg ccgacaagag ctcctccacc gcgtatatgc agttgagctc cctgacctcc     1140

gaggactccg ccgactacta ctgcgcacgg tccaactact atggaagctc gtactggttc     1200

ttcgatgtct ggggggccgg caccactgtg accgtcagct ccgggggcgg aggatccggt     1260

ggaggcggaa gcgggggtgg aggatccgac attgtgctga ctcagtcccc ggcaatcctg     1320

tcggcctcac cgggcgaaaa ggtcacgatg acttgtagag cgtcgtccag cgtgaactac     1380

atggattggt accaaaagaa gcctggatcg tcacccaagc cttggatcta cgctacatct     1440

aacctggcct ccggcgtgcc agcgcggttc agcgggtccg gctcgggcac ctcatactcg     1500

ctgaccatct cccgcgtgga ggctgaggac gccgcgacct actactgcca gcagtggtcc     1560

ttcaacccgc cgacttttgg aggcggtact aagctggaga tcaaagcggc cgcaactacc     1620

acccctgccc ctcggccgcc gactccggcc ccaaccatcg caagccaacc cctctccttg     1680

cgccccgaag cttgccgccc ggccgcgggt ggagccgtgc atacccgggg gctggacttt     1740

gcctgcgata tctacatttg ggccccgctg gccggcactt gcggcgtgct cctgctgtcg     1800

ctggtcatca ccctttactg caagaggggc cggaagaagc tgctttacat cttcaagcag     1860

ccgttcatgc ggcccgtgca gacgactcag gaagaggacg gatgctcgtg cagattccct     1920

gaggaggaag aggggggatg cgaactgcgc gtcaagttct cacggtccgc cgacgccccc     1980

gcatatcaac agggccagaa tcagctctac aacgagctga acctgggaag gagagaggag     2040

tacgacgtgc tggacaagcg acgcggacgc gacccggaga tgggggggaa accacggcgg     2100

aaaaaccctc aggaaggact gtacaacgaa ctccagaaag acaagatggc ggaagcctac     2160

tcagaaatcg ggatgaaggg agagcggagg aggggaaagg gtcacgacgg gctgtaccag     2220

ggactgagca ccgccactaa ggatacctac gatgccttgc atatgcaagc actcccaccc     2280

cgg                                                                   2283


<210>  2
<211>  761
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader-CD19 VL-Whitlow linker CD19 VH (GGGGS)-5 CD20 VH (GGGGS)-3
       CD20 VL CD8 hinge+TM-4-1BB- CD3z (Construct CAR 1920)

<400>  2

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
    50                  55                  60                  


Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 
            100                 105                 110         


Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser 
    130                 135                 140                 


Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala 
145                 150                 155                 160 


Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu 
                165                 170                 175     


Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu 
            180                 185                 190         


Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser 
        195                 200                 205             


Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln 
    210                 215                 220                 


Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr 
225                 230                 235                 240 


Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
            260                 265                 270         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        275                 280                 285             


Gly Gly Gly Ser Glu Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val 
    290                 295                 300                 


Lys Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr 
305                 310                 315                 320 


Phe Thr Ser Tyr Asn Met His Trp Val Lys Gln Thr Pro Gly Gln Gly 
                325                 330                 335     


Leu Glu Trp Ile Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr 
            340                 345                 350         


Asn Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser 
        355                 360                 365             


Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala 
    370                 375                 380                 


Asp Tyr Tyr Cys Ala Arg Ser Asn Tyr Tyr Gly Ser Ser Tyr Trp Phe 
385                 390                 395                 400 


Phe Asp Val Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ser Gly Gly 
                405                 410                 415     


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val 
            420                 425                 430         


Leu Thr Gln Ser Pro Ala Ile Leu Ser Ala Ser Pro Gly Glu Lys Val 
        435                 440                 445             


Thr Met Thr Cys Arg Ala Ser Ser Ser Val Asn Tyr Met Asp Trp Tyr 
    450                 455                 460                 


Gln Lys Lys Pro Gly Ser Ser Pro Lys Pro Trp Ile Tyr Ala Thr Ser 
465                 470                 475                 480 


Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly 
                485                 490                 495     


Thr Ser Tyr Ser Leu Thr Ile Ser Arg Val Glu Ala Glu Asp Ala Ala 
            500                 505                 510         


Thr Tyr Tyr Cys Gln Gln Trp Ser Phe Asn Pro Pro Thr Phe Gly Gly 
        515                 520                 525             


Gly Thr Lys Leu Glu Ile Lys Ala Ala Ala Thr Thr Thr Pro Ala Pro 
    530                 535                 540                 


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
545                 550                 555                 560 


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
                565                 570                 575     


Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
            580                 585                 590         


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys 
        595                 600                 605             


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
    610                 615                 620                 


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
625                 630                 635                 640 


Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser 
                645                 650                 655     


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
            660                 665                 670         


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
        675                 680                 685             


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
    690                 695                 700                 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
705                 710                 715                 720 


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
                725                 730                 735     


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
            740                 745                 750         


Leu His Met Gln Ala Leu Pro Pro Arg 
        755                 760     


<210>  3
<211>  2283
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Leader-CD20 VH (GGGGS)3 - CD20 VL -(GGGGS)5 -CD19 VL-Whitlow 
       linker- CD19 VH CD8 hinge+TM-4-1BB- CD3z (Construct 2019)

<400>  3
atgctccttc tcgtgacctc cctgcttctc tgcgaactgc cccatcctgc cttcctgctg       60

attcccgagg tgcagttgca acagtcagga gctgaactgg tcaagccagg agccagcgtg      120

aagatgagct gcaaggcctc cggttacacc ttcacctcct acaacatgca ctgggtgaaa      180

cagaccccgg gacaagggct cgaatggatt ggcgccatct accccgggaa tggcgatact      240

tcgtacaacc agaagttcaa gggaaaggcc accctgaccg ccgacaagag ctcctccacc      300

gcgtatatgc agttgagctc cctgacctcc gaggactccg ccgactacta ctgcgcacgg      360

tccaactact atggaagctc gtactggttc ttcgatgtct ggggggccgg caccactgtg      420

accgtcagct ccgggggcgg aggatccggt ggaggcggaa gcgggggtgg aggatccgac      480

attgtgctga ctcagtcccc ggcaatcctg tcggcctcac cgggcgaaaa ggtcacgatg      540

acttgtagag cgtcgtccag cgtgaactac atggattggt accaaaagaa gcctggatcg      600

tcacccaagc cttggatcta cgctacatct aacctggcct ccggcgtgcc agcgcggttc      660

agcgggtccg gctcgggcac ctcatactcg ctgaccatct cccgcgtgga ggctgaggac      720

gccgcgacct actactgcca gcagtggtcc ttcaacccgc cgacttttgg aggcggtact      780

aagctggaga tcaaaggagg cggcggcagc ggcgggggag ggtccggagg gggtggttct      840

ggtggaggag gatcgggagg cggtggcagc gacattcaga tgactcagac cacctcctcc      900

ctgtccgcct ccctgggcga ccgcgtgacc atctcatgcc gcgccagcca ggacatctcg      960

aagtacctca actggtacca gcagaagccc gacggaaccg tgaagctcct gatctaccac     1020

acctcccggc tgcacagcgg agtgccgtct agattctcgg gttcggggtc gggaactgac     1080

tactccctta ctatttccaa cctggagcag gaggatattg ccacctactt ctgccaacaa     1140

ggaaacaccc tgccgtacac ttttggcggg ggaaccaagc tggaaatcac tggcagcaca     1200

tccggttccg ggaagcccgg ctccggagag ggcagcacca agggggaagt caagctgcag     1260

gaatcaggac ctggcctggt ggccccgagc cagtcactgt ccgtgacttg tactgtgtcc     1320

ggagtgtcgc tcccggatta cggagtgtcc tggatcaggc agccacctcg gaaaggattg     1380

gaatggctcg gagtcatctg gggttccgaa accacctatt acaactcggc actgaaatcc     1440

aggctcacca ttatcaagga taactccaag tcacaagtgt tcctgaagat gaatagcctg     1500

cagactgacg acacggcgat ctactattgc gccaagcact actactacgg cggatcctac     1560

gctatggact actggggcca ggggaccagc gtgaccgtgt catccgcggc cgcaactacc     1620

acccctgccc ctcggccgcc gactccggcc ccaaccatcg caagccaacc cctctccttg     1680

cgccccgaag cttgccgccc ggccgcgggt ggagccgtgc atacccgggg gctggacttt     1740

gcctgcgata tctacatttg ggccccgctg gccggcactt gcggcgtgct cctgctgtcg     1800

ctggtcatca ccctttactg caagaggggc cggaagaagc tgctttacat cttcaagcag     1860

ccgttcatgc ggcccgtgca gacgactcag gaagaggacg gatgctcgtg cagattccct     1920

gaggaggaag aggggggatg cgaactgcgc gtcaagttct cacggtccgc cgacgccccc     1980

gcatatcaac agggccagaa tcagctctac aacgagctga acctgggaag gagagaggag     2040

tacgacgtgc tggacaagcg acgcggacgc gacccggaga tgggggggaa accacggcgg     2100

aaaaaccctc aggaaggact gtacaacgaa ctccagaaag acaagatggc ggaagcctac     2160

tcagaaatcg ggatgaaggg agagcggagg aggggaaagg gtcacgacgg gctgtaccag     2220

ggactgagca ccgccactaa ggatacctac gatgccttgc atatgcaagc actcccaccc     2280

cgg                                                                   2283


<210>  4
<211>  764
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader-CD20 VH (GGGGS)3 - CD20 VL -(GGGGS)5 -CD19 VL-Whitlow 
       linker- CD19 VH CD8 hinge+TM-4-1BB- CD3z amino acid sequence  
       (Construct CAR 2019)

<400>  4

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Glu Val Gln Leu Gln Gln Ser Gly Ala Glu 
            20                  25                  30          


Leu Val Lys Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Ser Tyr Asn Met His Trp Val Lys Gln Thr Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Ile Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr 
65                  70                  75                  80  


Ser Tyr Asn Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys 
                85                  90                  95      


Ser Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp 
            100                 105                 110         


Ser Ala Asp Tyr Tyr Cys Ala Arg Ser Asn Tyr Tyr Gly Ser Ser Tyr 
        115                 120                 125             


Trp Phe Phe Asp Val Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
145                 150                 155                 160 


Ile Val Leu Thr Gln Ser Pro Ala Ile Leu Ser Ala Ser Pro Gly Glu 
                165                 170                 175     


Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Asn Tyr Met Asp 
            180                 185                 190         


Trp Tyr Gln Lys Lys Pro Gly Ser Ser Pro Lys Pro Trp Ile Tyr Ala 
        195                 200                 205             


Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 


Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Val Glu Ala Glu Asp 
225                 230                 235                 240 


Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Phe Asn Pro Pro Thr Phe 
                245                 250                 255     


Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly 
            260                 265                 270         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        275                 280                 285             


Gly Ser Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser 
    290                 295                 300                 


Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser 
305                 310                 315                 320 


Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu 
                325                 330                 335     


Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe 
            340                 345                 350         


Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu 
        355                 360                 365             


Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu 
    370                 375                 380                 


Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Ser Thr 
385                 390                 395                 400 


Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Glu 
                405                 410                 415     


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
            420                 425                 430         


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
        435                 440                 445             


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
    450                 455                 460                 


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
465                 470                 475                 480 


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
                485                 490                 495     


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
            500                 505                 510         


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
        515                 520                 525             


Thr Ser Val Thr Val Ser Ser Ala Ala Ala Thr Thr Thr Pro Ala Pro 
    530                 535                 540                 


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
545                 550                 555                 560 


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
                565                 570                 575     


Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
            580                 585                 590         


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys 
        595                 600                 605             


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
    610                 615                 620                 


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
625                 630                 635                 640 


Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser 
                645                 650                 655     


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
            660                 665                 670         


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
        675                 680                 685             


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
    690                 695                 700                 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
705                 710                 715                 720 


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
                725                 730                 735     


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
            740                 745                 750         


Leu His Met Gln Ala Leu Pro Pro Arg Pro Arg Thr 
        755                 760                 


<210>  5
<211>  1491
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of CD19 LTG1494 (CAR 19A)

<400>  5
atggtcatgc ttctcctggt cacctccctg ctcctctgcg aactgcctca ccctgccttc       60

cttctgattc ctgacactga cattcagatg actcagacca cctcttcctt gtccgcgtca      120

ctgggagaca gagtgaccat ctcgtgtcgc gcaagccagg atatctccaa gtacctgaac      180

tggtaccaac agaagcccga cgggactgtg aagctgctga tctaccacac ctcacgcctg      240

cacagcggag tgccaagcag attctccggc tccggctcgg gaaccgatta ctcgcttacc      300

attagcaacc tcgagcagga ggacatcgct acctacttct gccagcaagg aaataccctg      360

ccctacacct tcggcggagg aaccaaattg gaaatcaccg gctccacgag cggctccggg      420

aagcctggtt ccggggaagg ctccactaag ggtgaagtga agctccagga gtccggcccc      480

ggcctggtgg cgccgtcgca atcactctct gtgacctgta ccgtgtcggg agtgtccctg      540

cctgattacg gcgtgagctg gattcggcag ccgccgcgga agggcctgga atggctgggt      600

gtcatctggg gatccgagac tacctactac aactcggccc tgaagtcccg cctgactatc      660

atcaaagaca actcgaagtc ccaggtcttt ctgaagatga actccctgca aactgacgac      720

accgccatct attactgtgc taagcactac tactacggtg gaagctatgc tatggactac      780

tggggccagg ggacatccgt gacagtcagc tccgcggccg caactaccac ccctgcccct      840

cggccgccga ctccggcccc aaccatcgca agccaacccc tctccttgcg ccccgaagct      900

tgccgcccgg ccgcgggtgg agccgtgcat acccgggggc tggactttgc ctgcgatatc      960

tacatttggg ccccgctggc cggcacttgc ggcgtgctcc tgctgtcgct ggtcatcacc     1020

ctttactgca agaggggccg gaagaagctg ctttacatct tcaagcagcc gttcatgcgg     1080

cccgtgcaga cgactcagga agaggacgga tgctcgtgca gattccctga ggaggaagag     1140

gggggatgcg aactgcgcgt caagttctca cggtccgccg acgcccccgc atatcaacag     1200

ggccagaatc agctctacaa cgagctgaac ctgggaagga gagaggagta cgacgtgctg     1260

gacaagcgac gcggacgcga cccggagatg ggggggaaac cacggcggaa aaaccctcag     1320

gaaggactgt acaacgaact ccagaaagac aagatggcgg aagcctactc agaaatcggg     1380

atgaagggag agcggaggag gggaaagggt cacgacgggc tgtaccaggg actgagcacc     1440

gccactaagg atacctacga tgccttgcat atgcaagcac tcccaccccg g              1491


<210>  6
<211>  496
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of CD19 LTG1494 (CAR 19A)

<400>  6

Met Val Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro 
1               5                   10                  15      


His Pro Ala Phe Leu Leu Ile Pro Asp Thr Asp Ile Gln Met Thr Gln 
            20                  25                  30          


Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser 
        35                  40                  45              


Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln 
    50                  55                  60                  


Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu 
65                  70                  75                  80  


His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
                85                  90                  95      


Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr 
            100                 105                 110         


Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr 
        115                 120                 125             


Lys Leu Glu Ile Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser 
    130                 135                 140                 


Gly Glu Gly Ser Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro 
145                 150                 155                 160 


Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser 
                165                 170                 175     


Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro 
            180                 185                 190         


Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr 
        195                 200                 205             


Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn 
    210                 215                 220                 


Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp 
225                 230                 235                 240 


Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr 
                245                 250                 255     


Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala 
            260                 265                 270         


Ala Ala Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
        275                 280                 285             


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
    290                 295                 300                 


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
305                 310                 315                 320 


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
                325                 330                 335     


Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
            340                 345                 350         


Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
        355                 360                 365             


Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
    370                 375                 380                 


Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
385                 390                 395                 400 


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
                405                 410                 415     


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
            420                 425                 430         


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
        435                 440                 445             


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
    450                 455                 460                 


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
465                 470                 475                 480 


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490                 495     


<210>  7
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of CD19 LTG1538 (CAR 19B)

<400>  7
atgcttctcc tggtcacctc cctgctcctc tgcgaactgc ctcaccctgc cttccttctg       60

attcctgaca ttcagatgac tcagaccacc tcttccttgt ccgcgtcact gggagacaga      120

gtgaccatct cgtgtcgcgc aagccaggat atctccaagt acctgaactg gtaccaacag      180

aagcccgacg ggactgtgaa gctgctgatc taccacacct cacgcctgca cagcggagtg      240

ccaagcagat tctccggctc cggctcggga accgattact cgcttaccat tagcaacctc      300

gagcaggagg acatcgctac ctacttctgc cagcaaggaa ataccctgcc ctacaccttc      360

ggcggaggaa ccaaattgga aatcaccggc ggaggaggct ccgggggagg aggttccggg      420

ggcgggggtt ccgaagtgaa gctccaggag tccggccccg gcctggtggc gccgtcgcaa      480

tcactctctg tgacctgtac cgtgtcggga gtgtccctgc ctgattacgg cgtgagctgg      540

attcggcagc cgccgcggaa gggcctggaa tggctgggtg tcatctgggg atccgagact      600

acctactaca actcggccct gaagtcccgc ctgactatca tcaaagacaa ctcgaagtcc      660

caggtctttc tgaagatgaa ctccctgcaa actgacgaca ccgccatcta ttactgtgct      720

aagcactact actacggtgg aagctatgct atggactact gggggcaagg cacttcggtg      780

actgtgtcaa gcgcggccgc aactaccacc cctgcccctc ggccgccgac tccggcccca      840

accatcgcaa gccaacccct ctccttgcgc cccgaagctt gccgcccggc cgcgggtgga      900

gccgtgcata cccgggggct ggactttgcc tgcgatatct acatttgggc cccgctggcc      960

ggcacttgcg gcgtgctcct gctgtcgctg gtcatcaccc tttactgcaa gaggggccgg     1020

aagaagctgc tttacatctt caagcagccg ttcatgcggc ccgtgcagac gactcaggaa     1080

gaggacggat gctcgtgcag attccctgag gaggaagagg ggggatgcga actgcgcgtc     1140

aagttctcac ggtccgccga cgcccccgca tatcaacagg gccagaatca gctctacaac     1200

gagctgaacc tgggaaggag agaggagtac gacgtgctgg acaagcgacg cggacgcgac     1260

ccggagatgg gggggaaacc acggcggaaa aaccctcagg aaggactgta caacgaactc     1320

cagaaagaca agatggcgga agcctactca gaaatcggga tgaagggaga gcggaggagg     1380

ggaaagggtc acgacgggct gtaccaggga ctgagcaccg ccactaagga tacctacgat     1440

gccttgcata tgcaagcact cccaccccgg                                      1470


<210>  8
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of CD19 LTG1538 (CAR 19B)

<400>  8

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
    50                  55                  60                  


Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 
            100                 105                 110         


Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
145                 150                 155                 160 


Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
                165                 170                 175     


Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu 
            180                 185                 190         


Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
        195                 200                 205             


Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
    210                 215                 220                 


Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala 
225                 230                 235                 240 


Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
                245                 250                 255     


Gly Thr Ser Val Thr Val Ser Ser Ala Ala Ala Thr Thr Thr Pro Ala 
            260                 265                 270         


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
        275                 280                 285             


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
    290                 295                 300                 


Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala 
305                 310                 315                 320 


Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys 
                325                 330                 335     


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
            340                 345                 350         


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
        355                 360                 365             


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
    370                 375                 380                 


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
385                 390                 395                 400 


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
                405                 410                 415     


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
            420                 425                 430         


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
        435                 440                 445             


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
    450                 455                 460                 


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
465                 470                 475                 480 


Ala Leu His Met Gln Ala Leu Pro Pro 
                485                 


<210>  9
<211>  1473
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of CD20A LTG1495 (CAR 20A)

<400>  9
atgctccttc tcgtgacctc cctgcttctc tgcgaactgc cccatcctgc cttcctgctg       60

attcccgagg tgcagttgca acagtcagga gctgaactgg tcaagccagg agccagcgtg      120

aagatgagct gcaaggcctc cggttacacc ttcacctcct acaacatgca ctgggtgaaa      180

cagaccccgg gacaagggct cgaatggatt ggcgccatct accccgggaa tggcgatact      240

tcgtacaacc agaagttcaa gggaaaggcc accctgaccg ccgacaagag ctcctccacc      300

gcgtatatgc agttgagctc cctgacctcc gaggactccg ccgactacta ctgcgcacgg      360

tccaactact atggaagctc gtactggttc ttcgatgtct ggggggccgg caccactgtg      420

accgtcagct ccgggggcgg aggatccggt ggaggcggaa gcgggggtgg aggatccgac      480

attgtgctga ctcagtcccc ggcaatcctg tcggcctcac cgggcgaaaa ggtcacgatg      540

acttgtagag cgtcgtccag cgtgaactac atggattggt accaaaagaa gcctggatcg      600

tcacccaagc cttggatcta cgctacatct aacctggcct ccggcgtgcc agcgcggttc      660

agcgggtccg gctcgggcac ctcatactcg ctgaccatct cccgcgtgga ggctgaggac      720

gccgcgacct actactgcca gcagtggtcc ttcaacccgc cgacttttgg aggcggtact      780

aagctggaga tcaaagcggc cgcaactacc acccctgccc ctcggccgcc gactccggcc      840

ccaaccatcg caagccaacc cctctccttg cgccccgaag cttgccgccc ggccgcgggt      900

ggagccgtgc atacccgggg gctggacttt gcctgcgata tctacatttg ggccccgctg      960

gccggcactt gcggcgtgct cctgctgtcg ctggtcatca ccctttactg caagaggggc     1020

cggaagaagc tgctttacat cttcaagcag ccgttcatgc ggcccgtgca gacgactcag     1080

gaagaggacg gatgctcgtg cagattccct gaggaggaag aggggggatg cgaactgcgc     1140

gtcaagttct cacggtccgc cgacgccccc gcatatcaac agggccagaa tcagctctac     1200

aacgagctga acctgggaag gagagaggag tacgacgtgc tggacaagcg acgcggacgc     1260

gacccggaga tgggggggaa accacggcgg aaaaaccctc aggaaggact gtacaacgaa     1320

ctccagaaag acaagatggc ggaagcctac tcagaaatcg ggatgaaggg agagcggagg     1380

aggggaaagg gtcacgacgg gctgtaccag ggactgagca ccgccactaa ggatacctac     1440

gatgccttgc atatgcaagc actcccaccc cgg                                  1473


<210>  10
<211>  491
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of CD20A 1495 (CAR 20A)

<400>  10

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Glu Val Gln Leu Gln Gln Ser Gly Ala Glu 
            20                  25                  30          


Leu Val Lys Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Ser Tyr Asn Met His Trp Val Lys Gln Thr Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Ile Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr 
65                  70                  75                  80  


Ser Tyr Asn Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys 
                85                  90                  95      


Ser Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp 
            100                 105                 110         


Ser Ala Asp Tyr Tyr Cys Ala Arg Ser Asn Tyr Tyr Gly Ser Ser Tyr 
        115                 120                 125             


Trp Phe Phe Asp Val Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
145                 150                 155                 160 


Ile Val Leu Thr Gln Ser Pro Ala Ile Leu Ser Ala Ser Pro Gly Glu 
                165                 170                 175     


Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Asn Tyr Met Asp 
            180                 185                 190         


Trp Tyr Gln Lys Lys Pro Gly Ser Ser Pro Lys Pro Trp Ile Tyr Ala 
        195                 200                 205             


Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 


Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Val Glu Ala Glu Asp 
225                 230                 235                 240 


Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Phe Asn Pro Pro Thr Phe 
                245                 250                 255     


Gly Gly Gly Thr Lys Leu Glu Ile Lys Ala Ala Ala Thr Thr Thr Pro 
            260                 265                 270         


Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu 
        275                 280                 285             


Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His 
    290                 295                 300                 


Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu 
305                 310                 315                 320 


Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr 
                325                 330                 335     


Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
            340                 345                 350         


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
        355                 360                 365             


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser 
    370                 375                 380                 


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
385                 390                 395                 400 


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
                405                 410                 415     


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
            420                 425                 430         


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
        435                 440                 445             


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
    450                 455                 460                 


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
465                 470                 475                 480 


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490     


<210>  11
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of leader/signal peptide sequence (LP)

<400>  11
atgctgctgc tggtgaccag cctgctgctg tgcgaactgc cgcatccggc gtttctgctg       60

attccg                                                                  66


<210>  12
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of leader/signal peptide sequence (LP)

<400>  12

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro 
            20          


<210>  13
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (GGGGS)3 linker

<400>  13

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  14
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (GGGGS)5 linker

<400>  14

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25  


<210>  15
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GGGGS linker

<400>  15

Gly Gly Gly Gly Ser 
1               5   


<210>  16

<400>  16
000

<210>  17

<400>  17
000

<210>  18

<400>  18
000

<210>  19

<400>  19
000

<210>  20

<400>  20
000

<210>  21

<400>  21
000

<210>  22

<400>  22
000

<210>  23

<400>  23
000

<210>  24

<400>  24
000

<210>  25

<400>  25
000

<210>  26

<400>  26
000

<210>  27

<400>  27
000

<210>  28

<400>  28
000

<210>  29

<400>  29
000

<210>  30

<400>  30
000

<210>  31

<400>  31
000

<210>  32

<400>  32
000

<210>  33

<400>  33
000

<210>  34

<400>  34
000

<210>  35
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of DNA CD8 transmembrane domain

<400>  35
atttgggccc cgctggccgg cacttgcggc gtgctcctgc tgtcgctggt catcaccctt       60

tactgc                                                                  66


<210>  36
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of CD8 transmembrane domain

<400>  36

Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu 
1               5                   10                  15      


Val Ile Thr Leu Tyr Cys 
            20          


<210>  37
<211>  141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of DNA CD8 hinge domain

<400>  37
actaccaccc ctgcccctcg gccgccgact ccggccccaa ccatcgcaag ccaacccctc       60

tccttgcgcc ccgaagcttg ccgcccggcc gcgggtggag ccgtgcatac ccgggggctg      120

gactttgcct gcgatatcta c                                                141


<210>  38
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of CD8 hinge domain

<400>  38

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr 
        35                  40                  45          


<210>  39
<211>  69
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of amino acid numbers 137 to 206 hinge and 
       transmembrane region of CD8.alpha. (NCBI RefSeq: 
       NP.sub.--001759.3)

<400>  39

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
        35                  40                  45              


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
    50                  55                  60                  


Ile Thr Leu Tyr Cys 
65                  


<210>  40
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of DNA signaling domain of 4-1BB

<400>  40
aagaggggcc ggaagaagct gctttacatc ttcaagcagc cgttcatgcg gcccgtgcag       60

acgactcagg aagaggacgg atgctcgtgc agattccctg aggaggaaga ggggggatgc      120

gaactg                                                                 126


<210>  41
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of signaling domain of 4-1BB

<400>  41

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  42
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of DNA signaling domain of CD3-zeta

<400>  42
cgcgtcaagt tctcacggtc cgccgacgcc cccgcatatc aacagggcca gaatcagctc       60

tacaacgagc tgaacctggg aaggagagag gagtacgacg tgctggacaa gcgacgcgga      120

cgcgacccgg agatgggggg gaaaccacgg cggaaaaacc ctcaggaagg actgtacaac      180

gaactccaga aagacaagat ggcggaagcc tactcagaaa tcgggatgaa gggagagcgg      240

aggaggggaa agggtcacga cgggctgtac cagggactga gcaccgccac taaggatacc      300

tacgatgcct tgcatatgca agcactccca ccccgg                                336


<210>  43
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of CD3zeta

<400>  43

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  44
<211>  726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of ScFv CD19 (FMC63)

<400>  44
gacattcaga tgactcagac cacctcttcc ttgtccgcgt cactgggaga cagagtgacc       60

atctcgtgtc gcgcaagcca ggatatctcc aagtacctga actggtacca acagaagccc      120

gacgggactg tgaagctgct gatctaccac acctcacgcc tgcacagcgg agtgccaagc      180

agattctccg gctccggctc gggaaccgat tactcgctta ccattagcaa cctcgagcag      240

gaggacatcg ctacctactt ctgccagcaa ggaaataccc tgccctacac cttcggcgga      300

ggaaccaaat tggaaatcac cggcggagga ggctccgggg gaggaggttc cgggggcggg      360

ggttccgaag tgaagctcca ggagtccggc cccggcctgg tggcgccgtc gcaatcactc      420

tctgtgacct gtaccgtgtc gggagtgtcc ctgcctgatt acggcgtgag ctggattcgg      480

cagccgccgc ggaagggcct ggaatggctg ggtgtcatct ggggatccga gactacctac      540

tacaactcgg ccctgaagtc ccgcctgact atcatcaaag acaactcgaa gtcccaggtc      600

tttctgaaga tgaactccct gcaaactgac gacaccgcca tctattactg tgctaagcac      660

tactactacg gtggaagcta tgctatggac tactgggggc aaggcacttc ggtgactgtg      720

tcaagc                                                                 726


<210>  45
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of ScFv CD19 (FMC63)

<400>  45

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu 
        115                 120                 125             


Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys 
    130                 135                 140                 


Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg 
145                 150                 155                 160 


Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser 
                165                 170                 175     


Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile 
            180                 185                 190         


Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 
        195                 200                 205             


Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly 
    210                 215                 220                 


Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 
225                 230                 235                 240 


Ser Ser 
        


<210>  46
<211>  1491
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of anti-CD33 CAR (LTG1936)

<400>  46
atgctgctgc tggtgaccag cctgctgctg tgcgaactgc cgcatccggc gtttctgctg       60

attccgcagg tgcagctggt gcaatctggg gcagaggtga aaaagcccgg ggagtctctg      120

aggatctcct gtaagggttc tggattcagt tttcccacct actggatcgg ctgggtgcgc      180

cagatgcccg ggaaaggcct ggagtggatg gggatcatct atcctggtga ctctgatacc      240

agatacagcc cgtccttcca aggccaggtc accatctcag ccgacaagtc catcagcacc      300

gcctacctgc agtggagcag cctgaaggcc tcggacaccg ccatgtatta ctgtgcgaga      360

ctagttggag atggctacaa tacgggggct tttgatatct ggggccaagg gacaatggtc      420

accgtctctt caggaggtgg cgggtctggt ggtggcggta gcggtggtgg cggatccgat      480

attgtgatga cccacactcc actctctctg tccgtcaccc ctggacagcc ggcctccatc      540

tcctgcaagt ctagtcagag cctcctgcat agtaatggaa agacctattt gtattggtac      600

ctgcagaagc caggccagcc tccacagctc ctgatctatg gagcttccaa ccggttctct      660

ggagtgccag acaggttcag tggcagcggg tcagggacag atttcacact gaaaatcagc      720

cgggtggagg ctgaggatgt tggggtttat tactgcatgc aaagtataca gcttcctatc      780

accttcggcc aagggacacg actggagatt aaagcggccg caactaccac ccctgcccct      840

cggccgccga ctccggcccc aaccatcgca agccaacccc tctccttgcg ccccgaagct      900

tgccgcccgg ccgcgggtgg agccgtgcat acccgggggc tggactttgc ctgcgatatc      960

tacatttggg ccccgctggc cggcacttgc ggcgtgctcc tgctgtcgct ggtcatcacc     1020

ctttactgca agaggggccg gaagaagctg ctttacatct tcaagcagcc gttcatgcgg     1080

cccgtgcaga cgactcagga agaggacgga tgctcgtgca gattccctga ggaggaagag     1140

gggggatgcg aactgcgcgt caagttctca cggtccgccg acgcccccgc atatcaacag     1200

ggccagaatc agctctacaa cgagctgaac ctgggaagga gagaggagta cgacgtgctg     1260

gacaagcgac gcggacgcga cccggagatg ggggggaaac cacggcggaa aaaccctcag     1320

gaaggactgt acaacgaact ccagaaagac aagatggcgg aagcctactc agaaatcggg     1380

atgaagggag agcggaggag gggaaagggt cacgacgggc tgtaccaggg actgagcacc     1440

gccactaagg atacctacga tgccttgcat atgcaagcac tcccaccccg g              1491


<210>  47
<211>  496
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of anti-CD33 CAR (LTG1936)

<400>  47

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Glu Ser Leu Arg Ile Ser Cys Lys Gly Ser Gly 
        35                  40                  45              


Phe Ser Phe Pro Thr Tyr Trp Ile Gly Trp Val Arg Gln Met Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Met Gly Ile Ile Tyr Pro Gly Asp Ser Asp Thr 
65                  70                  75                  80  


Arg Tyr Ser Pro Ser Phe Gln Gly Gln Val Thr Ile Ser Ala Asp Lys 
                85                  90                  95      


Ser Ile Ser Thr Ala Tyr Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp 
            100                 105                 110         


Thr Ala Met Tyr Tyr Cys Ala Arg Leu Val Gly Asp Gly Tyr Asn Thr 
        115                 120                 125             


Gly Ala Phe Asp Ile Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
145                 150                 155                 160 


Ile Val Met Thr His Thr Pro Leu Ser Leu Ser Val Thr Pro Gly Gln 
                165                 170                 175     


Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu His Ser Asn 
            180                 185                 190         


Gly Lys Thr Tyr Leu Tyr Trp Tyr Leu Gln Lys Pro Gly Gln Pro Pro 
        195                 200                 205             


Gln Leu Leu Ile Tyr Gly Ala Ser Asn Arg Phe Ser Gly Val Pro Asp 
    210                 215                 220                 


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser 
225                 230                 235                 240 


Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln Ser Ile 
                245                 250                 255     


Gln Leu Pro Ile Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys Ala 
            260                 265                 270         


Ala Ala Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
        275                 280                 285             


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
    290                 295                 300                 


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
305                 310                 315                 320 


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
                325                 330                 335     


Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
            340                 345                 350         


Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
        355                 360                 365             


Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
    370                 375                 380                 


Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
385                 390                 395                 400 


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
                405                 410                 415     


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
            420                 425                 430         


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
        435                 440                 445             


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
    450                 455                 460                 


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
465                 470                 475                 480 


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
                485                 490                 495     


<210>  48
<211>  1476
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of anti-mesothelin CAR (LTG1904)

<400>  48
atgctgctgc tggtgaccag cctgctgctg tgcgaactgc cgcatccggc gtttctgctg       60

attccggagg tccagctggt acagtctggg ggaggcttgg tacagcctgg ggggtccctg      120

agactctcct gtgcagcctc tggattcacc tttgatgatt atgccatgca ctgggtccgg      180

caagctccag ggaagggcct ggagtgggtc tcaggtatta gttggaatag tggtagcata      240

ggctatgcgg actctgtgaa gggccgattc accatctcca gagacaacgc caagaactcc      300

ctgtatctgc aaatgaacag tctgagagct gaggacacgg ccttgtatta ctgtgcaaaa      360

gatttatcgt cagtggctgg accctttaac tactggggcc agggcaccct ggtcaccgtc      420

tcctcaggag gtggcgggtc tggtggaggc ggtagcggcg gtggcggatc ctcttctgag      480

ctgactcagg accctgctgt gtctgtggcc ttgggacaga cagtcaggat cacatgccaa      540

ggagacagcc tcagaagcta ttatgcaagc tggtaccagc agaagccagg acaggcccct      600

gtacttgtca tctatggtaa aaacaaccgg ccctcaggga tcccagaccg attctctggc      660

tccagctcag gaaacacagc ttccttgacc atcactgggg ctcaggcgga ggatgaggct      720

gactattact gtaactcccg ggacagcagt ggtaaccatc tggtattcgg cggaggcacc      780

cagctgaccg tcctcggtgc ggccgcaact accacccctg cccctcggcc gccgactccg      840

gccccaacca tcgcaagcca acccctctcc ttgcgccccg aagcttgccg cccggccgcg      900

ggtggagccg tgcatacccg ggggctggac tttgcctgcg atatctacat ttgggccccg      960

ctggccggca cttgcggcgt gctcctgctg tcgctggtca tcacccttta ctgcaagagg     1020

ggccggaaga agctgcttta catcttcaag cagccgttca tgcggcccgt gcagacgact     1080

caggaagagg acggatgctc gtgcagattc cctgaggagg aagagggggg atgcgaactg     1140

cgcgtcaagt tctcacggtc cgccgacgcc cccgcatatc aacagggcca gaatcagctc     1200

tacaacgagc tgaacctggg aaggagagag gagtacgacg tgctggacaa gcgacgcgga     1260

cgcgacccgg agatgggggg gaaaccacgg cggaaaaacc ctcaggaagg actgtacaac     1320

gaactccaga aagacaagat ggcggaagcc tactcagaaa tcgggatgaa gggagagcgg     1380

aggaggggaa agggtcacga cgggctgtac cagggactga gcaccgccac taaggatacc     1440

tacgatgcct tgcatatgca agcactccca ccccgg                               1476


<210>  49
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of anti-mesothelin CAR (LTG1904)

<400>  49

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Glu Val Gln Leu Val Gln Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Asp Asp Tyr Ala Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Ser Gly Ile Ser Trp Asn Ser Gly Ser Ile 
65                  70                  75                  80  


Gly Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Leu Tyr Tyr Cys Ala Lys Asp Leu Ser Ser Val Ala Gly Pro 
        115                 120                 125             


Phe Asn Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Ser Glu 
145                 150                 155                 160 


Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln Thr Val Arg 
                165                 170                 175     


Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala Ser Trp Tyr 
            180                 185                 190         


Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr Gly Lys Asn 
        195                 200                 205             


Asn Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly 
    210                 215                 220                 


Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu Asp Glu Ala 
225                 230                 235                 240 


Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Gly Asn His Leu Val Phe 
                245                 250                 255     


Gly Gly Gly Thr Gln Leu Thr Val Leu Gly Ala Ala Ala Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro 
305                 310                 315                 320 


Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu 
                325                 330                 335     


Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro 
            340                 345                 350         


Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys 
        355                 360                 365             


Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe 
    370                 375                 380                 


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
385                 390                 395                 400 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
                405                 410                 415     


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
            420                 425                 430         


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
        435                 440                 445             


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
    450                 455                 460                 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
465                 470                 475                 480 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490         


