               SEQUENCE LISTING

<110> Barentzymes AS

<120> Neutral Heat-Sensitive Serine Protease derived from O. corvina

<130> DG018133-P-WO

<150> EP17185519
<151> 2017-08-09

<160> 11

<170> BiSSAP 1.3.2

<210> 1
<211> 1209
<212> DNA
<213> Artificial Sequence

<223> "CDS 1...1209"

<220> 
<223> CDS 1...1209

<400> 1
atgggttgta tcaaggttat ctccgttttc ttggctgctg ttgctgctgt tgacgctaga      60

gcttttttcc acaacaaggg tggtaacgac atcatcccaa actcctacat cgttgttatg     120

aaggacggta tctccactca agagttcgag tcccacattt cttccgtttc tgctactcac     180

gctaagagat ccactgagtt ggttggtcac aaggattcct tcaacatcaa cggttggaga     240

gcttacaacg gtcacttcga tgctgctact ttggagtcca ttttgaacga cgacaacgtt     300

aagtacgttg agcacgacag agttgttaag atctccgctt tgactactca gccaaacgct     360

ccatcttggg gtttgggtag aatttctcac agatccccag gtaacaagga cttcgtttac     420

gatgacactg ctggtcaggg tatcactatc tacggtgttg acactggaat cgacatcaac     480

cacccagatt ttggtggtag agctagatgg ggaactaaca ctgttgacaa cgctaacaac     540

gacggtcatg gtcatggtac tcatactgct ggtactttcg ctggtaacgc ttacggtatt     600

gctaagaagg cttccgttgt tgctgttaag gttttgtctg cttctggttc cggttccaac     660

gctggtgtta ttaagggtat cgactggtgt gttacagacg ctagatctaa gggtgctttg     720

ggtaaggctg ctttgaactt gtctttgggt ggtggtttca accaggctac aaacgacgct     780

gttactagag ctcagactgc tggaatcttc gttgctgttg ctgctggtaa cgataacaag     840

gacgctagaa actactctcc agcttctgct ccagctgttt gtactgttgc ttcctccact     900

atcgacgacc aaaagtcctc tttctctaac tggggttcca tcgttgacat ctacgctcca     960

ggttccaaca ttatttccga tgctccaggt ggtggtgtta gaactatgtc tggtacttct    1020

atggcttccc cacacgtttg tggtgctggt gctgctatgt tggctcaagg tgttccagtt    1080

ggtcaggttt gtgacagatt gaagcagatc ggtaacgctg ttgttagaaa cccaggtact    1140

tccactacaa acagattgtt gtataacggt tccggtcagg gttctcatca tcaccatcac    1200

cactaatag                                                            1209


<210> 2
<211> 401
<212> PRT
<213> Onygena corvina


<400> 2
Met Gly Cys Ile Lys Val Ile Ser Val Phe Leu Ala Ala Val Ala Ala 
1               5                   10                  15      
Val Asp Ala Arg Ala Phe Phe His Asn Lys Gly Gly Asn Asp Ile Ile 
            20                  25                  30          
Pro Asn Ser Tyr Ile Val Val Met Lys Asp Gly Ile Ser Thr Gln Glu 
        35                  40                  45              
Phe Glu Ser His Ile Ser Ser Val Ser Ala Thr His Ala Lys Arg Ser 
    50                  55                  60                  
Thr Glu Leu Val Gly His Lys Asp Ser Phe Asn Ile Asn Gly Trp Arg 
65                  70                  75                  80  
Ala Tyr Asn Gly His Phe Asp Ala Ala Thr Leu Glu Ser Ile Leu Asn 
                85                  90                  95      
Asp Asp Asn Val Lys Tyr Val Glu His Asp Arg Val Val Lys Ile Ser 
            100                 105                 110         
Ala Leu Thr Thr Gln Pro Asn Ala Pro Ser Trp Gly Leu Gly Arg Ile 
        115                 120                 125             
Ser His Arg Ser Pro Gly Asn Lys Asp Phe Val Tyr Asp Asp Thr Ala 
    130                 135                 140                 
Gly Gln Gly Ile Thr Ile Tyr Gly Val Asp Thr Gly Ile Asp Ile Asn 
145                 150                 155                 160 
His Pro Asp Phe Gly Gly Arg Ala Arg Trp Gly Thr Asn Thr Val Asp 
                165                 170                 175     
Asn Ala Asn Asn Asp Gly His Gly His Gly Thr His Thr Ala Gly Thr 
            180                 185                 190         
Phe Ala Gly Asn Ala Tyr Gly Ile Ala Lys Lys Ala Ser Val Val Ala 
        195                 200                 205             
Val Lys Val Leu Ser Ala Ser Gly Ser Gly Ser Asn Ala Gly Val Ile 
    210                 215                 220                 
Lys Gly Ile Asp Trp Cys Val Thr Asp Ala Arg Ser Lys Gly Ala Leu 
225                 230                 235                 240 
Gly Lys Ala Ala Leu Asn Leu Ser Leu Gly Gly Gly Phe Asn Gln Ala 
                245                 250                 255     
Thr Asn Asp Ala Val Thr Arg Ala Gln Thr Ala Gly Ile Phe Val Ala 
            260                 265                 270         
Val Ala Ala Gly Asn Asp Asn Lys Asp Ala Arg Asn Tyr Ser Pro Ala 
        275                 280                 285             
Ser Ala Pro Ala Val Cys Thr Val Ala Ser Ser Thr Ile Asp Asp Gln 
    290                 295                 300                 
Lys Ser Ser Phe Ser Asn Trp Gly Ser Ile Val Asp Ile Tyr Ala Pro 
305                 310                 315                 320 
Gly Ser Asn Ile Ile Ser Asp Ala Pro Gly Gly Gly Val Arg Thr Met 
                325                 330                 335     
Ser Gly Thr Ser Met Ala Ser Pro His Val Cys Gly Ala Gly Ala Ala 
            340                 345                 350         
Met Leu Ala Gln Gly Val Pro Val Gly Gln Val Cys Asp Arg Leu Lys 
        355                 360                 365             
Gln Ile Gly Asn Ala Val Val Arg Asn Pro Gly Thr Ser Thr Thr Asn 
    370                 375                 380                 
Arg Leu Leu Tyr Asn Gly Ser Gly Gln Gly Ser His His His His His 
385                 390                 395                 400 
His 
    

<210> 3
<211> 837
<212> DNA
<213> Artificial Sequence

<223> "CDS 1...837"

<220> 
<223> CDS 1...837

<400> 3
gctttgacta ctcagccaaa cgctccatct tggggtttgg gtagaatttc tcacagatcc      60

ccaggtaaca aggacttcgt ttacgatgac actgctggtc agggtatcac tatctacggt     120

gttgacactg gaatcgacat caaccaccca gattttggtg gtagagctag atggggaact     180

aacactgttg acaacgctaa caacgacggt catggtcatg gtactcatac tgctggtact     240

ttcgctggta acgcttacgg tattgctaag aaggcttccg ttgttgctgt taaggttttg     300

tctgcttctg gttccggttc caacgctggt gttattaagg gtatcgactg gtgtgttaca     360

gacgctagat ctaagggtgc tttgggtaag gctgctttga acttgtcttt gggtggtggt     420

ttcaaccagg ctacaaacga cgctgttact agagctcaga ctgctggaat cttcgttgct     480

gttgctgctg gtaacgataa caaggacgct agaaactact ctccagcttc tgctccagct     540

gtttgtactg ttgcttcctc cactatcgac gaccaaaagt cctctttctc taactggggt     600

tccatcgttg acatctacgc tccaggttcc aacattattt ccgatgctcc aggtggtggt     660

gttagaacta tgtctggtac ttctatggct tccccacacg tttgtggtgc tggtgctgct     720

atgttggctc aaggtgttcc agttggtcag gtttgtgaca gattgaagca gatcggtaac     780

gctgttgtta gaaacccagg tacttccact acaaacagat tgttgtataa cggttcc        837


<210> 4
<211> 279
<212> PRT
<213> Onygena corvina


<400> 4
Ala Leu Thr Thr Gln Pro Asn Ala Pro Ser Trp Gly Leu Gly Arg Ile 
1               5                   10                  15      
Ser His Arg Ser Pro Gly Asn Lys Asp Phe Val Tyr Asp Asp Thr Ala 
            20                  25                  30          
Gly Gln Gly Ile Thr Ile Tyr Gly Val Asp Thr Gly Ile Asp Ile Asn 
        35                  40                  45              
His Pro Asp Phe Gly Gly Arg Ala Arg Trp Gly Thr Asn Thr Val Asp 
    50                  55                  60                  
Asn Ala Asn Asn Asp Gly His Gly His Gly Thr His Thr Ala Gly Thr 
65                  70                  75                  80  
Phe Ala Gly Asn Ala Tyr Gly Ile Ala Lys Lys Ala Ser Val Val Ala 
                85                  90                  95      
Val Lys Val Leu Ser Ala Ser Gly Ser Gly Ser Asn Ala Gly Val Ile 
            100                 105                 110         
Lys Gly Ile Asp Trp Cys Val Thr Asp Ala Arg Ser Lys Gly Ala Leu 
        115                 120                 125             
Gly Lys Ala Ala Leu Asn Leu Ser Leu Gly Gly Gly Phe Asn Gln Ala 
    130                 135                 140                 
Thr Asn Asp Ala Val Thr Arg Ala Gln Thr Ala Gly Ile Phe Val Ala 
145                 150                 155                 160 
Val Ala Ala Gly Asn Asp Asn Lys Asp Ala Arg Asn Tyr Ser Pro Ala 
                165                 170                 175     
Ser Ala Pro Ala Val Cys Thr Val Ala Ser Ser Thr Ile Asp Asp Gln 
            180                 185                 190         
Lys Ser Ser Phe Ser Asn Trp Gly Ser Ile Val Asp Ile Tyr Ala Pro 
        195                 200                 205             
Gly Ser Asn Ile Ile Ser Asp Ala Pro Gly Gly Gly Val Arg Thr Met 
    210                 215                 220                 
Ser Gly Thr Ser Met Ala Ser Pro His Val Cys Gly Ala Gly Ala Ala 
225                 230                 235                 240 
Met Leu Ala Gln Gly Val Pro Val Gly Gln Val Cys Asp Arg Leu Lys 
                245                 250                 255     
Gln Ile Gly Asn Ala Val Val Arg Asn Pro Gly Thr Ser Thr Thr Asn 
            260                 265                 270         
Arg Leu Leu Tyr Asn Gly Ser 
        275                 

<210> 5
<211> 21
<212> DNA
<213> Artificial Sequence

<223> "Forward Primer P1"

<220> 
<223> Forward Primer P1

<400> 5
gactggttcc aattgacaag c                                              21


<210> 6
<211> 19
<212> DNA
<213> Artificial Sequence

<223> "Reversed Primer P2"

<220> 
<223> Reversed Primer P2

<400> 6
gcgtgaatgt aagcgtgac                                                 19


<210> 7
<211> 8
<212> PRT
<213> Artificial Sequence

<223> "N-terminus of mature NHSSP"

<220> 
<223> N-terminus of mature NHSSP

<400> 7
Ala Leu Thr Thr Gln Pro Asn Ala 
1               5               

<210> 8
<211> 1179
<212> DNA
<213> Artificial Sequence

<223> "CDS 1 ...1179"

<220> 
<223> CDS 1 ...1179

<400> 8
atgggttgta tcaaggttat ctccgttttc ttggctgctg ttgctgctgt tgacgctaga      60

gcttttttcc acaacaaggg tggtaacgac atcatcccaa actcctacat cgttgttatg     120

aaggacggta tctccactca agagttcgag tcccacattt cttccgtttc tgctactcac     180

gctaagagat ccactgagtt ggttggtcac aaggattcct tcaacatcaa cggttggaga     240

gcttacaacg gtcacttcga tgctgctact ttggagtcca ttttgaacga cgacaacgtt     300

aagtacgttg agcacgacag agttgttaag atctccgctt tgactactca gccaaacgct     360

ccatcttggg gtttgggtag aatttctcac agatccccag gtaacaagga cttcgtttac     420

gatgacactg ctggtcaggg tatcactatc tacggtgttg acactggaat cgacatcaac     480

cacccagatt ttggtggtag agctagatgg ggaactaaca ctgttgacaa cgctaacaac     540

gacggtcatg gtcatggtac tcatactgct ggtactttcg ctggtaacgc ttacggtatt     600

gctaagaagg cttccgttgt tgctgttaag gttttgtctg cttctggttc cggttccaac     660

gctggtgtta ttaagggtat cgactggtgt gttacagacg ctagatctaa gggtgctttg     720

ggtaaggctg ctttgaactt gtctttgggt ggtggtttca accaggctac aaacgacgct     780

gttactagag ctcagactgc tggaatcttc gttgctgttg ctgctggtaa cgataacaag     840

gacgctagaa actactctcc agcttctgct ccagctgttt gtactgttgc ttcctccact     900

atcgacgacc aaaagtcctc tttctctaac tggggttcca tcgttgacat ctacgctcca     960

ggttccaaca ttatttccga tgctccaggt ggtggtgtta gaactatgtc tggtacttct    1020

atggcttccc cacacgtttg tggtgctggt gctgctatgt tggctcaagg tgttccagtt    1080

ggtcaggttt gtgacagatt gaagcagatc ggtaacgctg ttgttagaaa cccaggtact    1140

tccactacaa acagattgtt gtataacggt tcctaatga                           1179


<210> 9
<211> 391
<212> PRT
<213> Artificial Sequence

<223> "Truncated NHSSP"

<220> 
<223> Truncated NHSSP

<400> 9
Met Gly Cys Ile Lys Val Ile Ser Val Phe Leu Ala Ala Val Ala Ala 
1               5                   10                  15      
Val Asp Ala Arg Ala Phe Phe His Asn Lys Gly Gly Asn Asp Ile Ile 
            20                  25                  30          
Pro Asn Ser Tyr Ile Val Val Met Lys Asp Gly Ile Ser Thr Gln Glu 
        35                  40                  45              
Phe Glu Ser His Ile Ser Ser Val Ser Ala Thr His Ala Lys Arg Ser 
    50                  55                  60                  
Thr Glu Leu Val Gly His Lys Asp Ser Phe Asn Ile Asn Gly Trp Arg 
65                  70                  75                  80  
Ala Tyr Asn Gly His Phe Asp Ala Ala Thr Leu Glu Ser Ile Leu Asn 
                85                  90                  95      
Asp Asp Asn Val Lys Tyr Val Glu His Asp Arg Val Val Lys Ile Ser 
            100                 105                 110         
Ala Leu Thr Thr Gln Pro Asn Ala Pro Ser Trp Gly Leu Gly Arg Ile 
        115                 120                 125             
Ser His Arg Ser Pro Gly Asn Lys Asp Phe Val Tyr Asp Asp Thr Ala 
    130                 135                 140                 
Gly Gln Gly Ile Thr Ile Tyr Gly Val Asp Thr Gly Ile Asp Ile Asn 
145                 150                 155                 160 
His Pro Asp Phe Gly Gly Arg Ala Arg Trp Gly Thr Asn Thr Val Asp 
                165                 170                 175     
Asn Ala Asn Asn Asp Gly His Gly His Gly Thr His Thr Ala Gly Thr 
            180                 185                 190         
Phe Ala Gly Asn Ala Tyr Gly Ile Ala Lys Lys Ala Ser Val Val Ala 
        195                 200                 205             
Val Lys Val Leu Ser Ala Ser Gly Ser Gly Ser Asn Ala Gly Val Ile 
    210                 215                 220                 
Lys Gly Ile Asp Trp Cys Val Thr Asp Ala Arg Ser Lys Gly Ala Leu 
225                 230                 235                 240 
Gly Lys Ala Ala Leu Asn Leu Ser Leu Gly Gly Gly Phe Asn Gln Ala 
                245                 250                 255     
Thr Asn Asp Ala Val Thr Arg Ala Gln Thr Ala Gly Ile Phe Val Ala 
            260                 265                 270         
Val Ala Ala Gly Asn Asp Asn Lys Asp Ala Arg Asn Tyr Ser Pro Ala 
        275                 280                 285             
Ser Ala Pro Ala Val Cys Thr Val Ala Ser Ser Thr Ile Asp Asp Gln 
    290                 295                 300                 
Lys Ser Ser Phe Ser Asn Trp Gly Ser Ile Val Asp Ile Tyr Ala Pro 
305                 310                 315                 320 
Gly Ser Asn Ile Ile Ser Asp Ala Pro Gly Gly Gly Val Arg Thr Met 
                325                 330                 335     
Ser Gly Thr Ser Met Ala Ser Pro His Val Cys Gly Ala Gly Ala Ala 
            340                 345                 350         
Met Leu Ala Gln Gly Val Pro Val Gly Gln Val Cys Asp Arg Leu Lys 
        355                 360                 365             
Gln Ile Gly Asn Ala Val Val Arg Asn Pro Gly Thr Ser Thr Thr Asn 
    370                 375                 380                 
Arg Leu Leu Tyr Asn Gly Ser 
385                 390     

<210> 10
<211> 31
<212> DNA
<213> Artificial Sequence

<223> "Forward primer P3"

<220> 
<223> Forward primer P3

<400> 10
gccagaattc atgggttgta tcaaggttat c                                   31


<210> 11
<211> 42
<212> DNA
<213> Artificial Sequence

<223> "Reversed primer P4"

<220> 
<223> Reversed primer P4

<400> 11
gccgggtacc tcattaggaa ccgttataca acaatctgtt tg                        42


