                         SEQUENCE LISTING

<110>  Danisco US Inc.
 
<120>  ALPHA-AMYLASE VARIANTS

<130>  NB41254-WO-PCT

<140>  PCT/US18/47090
<141>  2018-08-20

<150>  US 62/547425  
<151>  2017-08-18

<150>  US 62/559179  
<151>  2017-09-15

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  485
<212>  PRT
<213>  Bacillus sp. 707

<400>  1

His His Asn Gly Thr Asn Gly Thr Met Met Gln Tyr Phe Glu Trp Tyr 
1               5                   10                  15      


Leu Pro Asn Asp Gly Asn His Trp Asn Arg Leu Asn Ser Asp Ala Ser 
            20                  25                  30          


Asn Leu Lys Ser Lys Gly Ile Thr Ala Val Trp Ile Pro Pro Ala Trp 
        35                  40                  45              


Lys Gly Ala Ser Gln Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 
    50                  55                  60                  


Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly 
65                  70                  75                  80  


Thr Arg Ser Gln Leu Gln Ala Ala Val Thr Ser Leu Lys Asn Asn Gly 
                85                  90                  95      


Ile Gln Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp 
            100                 105                 110         


Ala Thr Glu Met Val Arg Ala Val Glu Val Asn Pro Asn Asn Arg Asn 
        115                 120                 125             


Gln Glu Val Thr Gly Glu Tyr Thr Ile Glu Ala Trp Thr Arg Phe Asp 
    130                 135                 140                 


Phe Pro Gly Arg Gly Asn Thr His Ser Ser Phe Lys Trp Arg Trp Tyr 
145                 150                 155                 160 


His Phe Asp Gly Val Asp Trp Asp Gln Ser Arg Arg Leu Asn Asn Arg 
                165                 170                 175     


Ile Tyr Lys Phe Arg Gly His Gly Lys Ala Trp Asp Trp Glu Val Asp 
            180                 185                 190         


Thr Glu Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Met 
        195                 200                 205             


Asp His Pro Glu Val Val Asn Glu Leu Arg Asn Trp Gly Val Trp Tyr 
    210                 215                 220                 


Thr Asn Thr Leu Gly Leu Asp Gly Phe Arg Ile Asp Ala Val Lys His 
225                 230                 235                 240 


Ile Lys Tyr Ser Phe Thr Arg Asp Trp Ile Asn His Val Arg Ser Ala 
                245                 250                 255     


Thr Gly Lys Asn Met Phe Ala Val Ala Glu Phe Trp Lys Asn Asp Leu 
            260                 265                 270         


Gly Ala Ile Glu Asn Tyr Leu Gln Lys Thr Asn Trp Asn His Ser Val 
        275                 280                 285             


Phe Asp Val Pro Leu His Tyr Asn Leu Tyr Asn Ala Ser Lys Ser Gly 
    290                 295                 300                 


Gly Asn Tyr Asp Met Arg Asn Ile Phe Asn Gly Thr Val Val Gln Arg 
305                 310                 315                 320 


His Pro Ser His Ala Val Thr Phe Val Asp Asn His Asp Ser Gln Pro 
                325                 330                 335     


Glu Glu Ala Leu Glu Ser Phe Val Glu Glu Trp Phe Lys Pro Leu Ala 
            340                 345                 350         


Tyr Ala Leu Thr Leu Thr Arg Glu Gln Gly Tyr Pro Ser Val Phe Tyr 
        355                 360                 365             


Gly Asp Tyr Tyr Gly Ile Pro Thr His Gly Val Pro Ala Met Arg Ser 
    370                 375                 380                 


Lys Ile Asp Pro Ile Leu Glu Ala Arg Gln Lys Tyr Ala Tyr Gly Lys 
385                 390                 395                 400 


Gln Asn Asp Tyr Leu Asp His His Asn Ile Ile Gly Trp Thr Arg Glu 
                405                 410                 415     


Gly Asn Thr Ala His Pro Asn Ser Gly Leu Ala Thr Ile Met Ser Asp 
            420                 425                 430         


Gly Ala Gly Gly Ser Lys Trp Met Phe Val Gly Arg Asn Lys Ala Gly 
        435                 440                 445             


Gln Val Trp Ser Asp Ile Thr Gly Asn Arg Thr Gly Thr Val Thr Ile 
    450                 455                 460                 


Asn Ala Asp Gly Trp Gly Asn Phe Ser Val Asn Gly Gly Ser Val Ser 
465                 470                 475                 480 


Ile Trp Val Asn Lys 
                485 


<210>  2
<211>  483
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of Amy707 alpha-amylase

<400>  2

His His Asn Gly Thr Asn Gly Thr Met Met Gln Tyr Phe Glu Trp Tyr 
1               5                   10                  15      


Leu Pro Asn Asp Gly Asn His Trp Asn Arg Leu Asn Ser Asp Ala Ser 
            20                  25                  30          


Asn Leu Lys Ser Lys Gly Ile Thr Ala Val Trp Ile Pro Pro Ala Tyr 
        35                  40                  45              


Lys Gly Thr Ser Gln Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 
    50                  55                  60                  


Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly 
65                  70                  75                  80  


Thr Arg Ser Gln Leu Gln Ala Ala Val Thr Ser Leu Lys Asn Asn Gly 
                85                  90                  95      


Ile Gln Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp 
            100                 105                 110         


Ala Thr Glu Met Val Arg Ala Val Glu Val Asn Pro Ser Asn Arg Asn 
        115                 120                 125             


Gln Glu Ile Ser Gly Asp Tyr Thr Ile Glu Ala Trp Thr Lys Phe Asp 
    130                 135                 140                 


Phe Pro Gly Arg Gly Asn Thr His Ser Ser Phe Lys Trp Arg Trp Tyr 
145                 150                 155                 160 


His Phe Asp Gly Val Asp Trp Asp Gln Ser Arg Arg Leu Asn Asn Arg 
                165                 170                 175     


Ile Tyr Lys Phe His Gly Lys Ala Trp Asp Trp Pro Val Ser Ser Glu 
            180                 185                 190         


Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Met Asp His 
        195                 200                 205             


Pro Asp Val Val Asn Glu Leu Arg Asn Trp Gly Val Trp Tyr Ala Asn 
    210                 215                 220                 


Thr Leu Gly Leu Asp Gly Phe Arg Ile Asp Ala Val Lys His Ile Lys 
225                 230                 235                 240 


Tyr Ser Phe Thr Arg Asp Trp Ile Asn His Val Arg Ser Ala Thr Gly 
                245                 250                 255     


Lys Asn Met Phe Ala Val Ala Glu Phe Trp Lys Asn Asp Leu Gly Ala 
            260                 265                 270         


Ile Glu Asn Tyr Leu Gln Lys Thr Asn Trp Asn His Ser Val Phe Asp 
        275                 280                 285             


Val Pro Leu His Tyr Asn Leu Tyr Asn Ala Ser Lys Ser Gly Gly Asn 
    290                 295                 300                 


Tyr Asp Met Arg Asn Ile Phe Asn Gly Thr Val Val Gln Arg His Pro 
305                 310                 315                 320 


Ser His Ala Val Thr Phe Val Asp Asn His Asp Ser Gln Pro Glu Glu 
                325                 330                 335     


Ala Leu Glu Ser Phe Val Glu Glu Trp Phe Lys Pro Leu Ala Tyr Ala 
            340                 345                 350         


Leu Thr Leu Thr Arg Glu Gln Gly Tyr Pro Ser Val Phe Tyr Gly Asp 
        355                 360                 365             


Tyr Tyr Gly Ile Pro Thr His Gly Val Pro Ala Met Arg Ser Lys Ile 
    370                 375                 380                 


Asp Pro Ile Leu Glu Ala Arg Gln Lys Tyr Ala Tyr Gly Lys Gln Asn 
385                 390                 395                 400 


Asp Tyr Leu Asp His His Asn Ile Ile Gly Trp Thr Arg Glu Gly Asn 
                405                 410                 415     


Thr Ala His Pro Asn Ser Gly Leu Ala Thr Ile Met Ser Asp Gly Ala 
            420                 425                 430         


Gly Gly Ser Lys Trp Met Asn Val Gly Lys Asn Asn Ala Gly Glu Val 
        435                 440                 445             


Trp Tyr Asp Ile Thr Gly Asn Arg Thr Gly Thr Val Thr Ile Asn Ala 
    450                 455                 460                 


Asp Gly Trp Gly Gln Phe His Val Asn Gly Gly Ser Val Ser Ile Trp 
465                 470                 475                 480 


Val Asn Lys 
            


<210>  3
<211>  483
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of Amy707 alpha-amylase

<400>  3

His His Asn Gly Thr Asn Gly Thr Met Met Gln Tyr Phe Glu Trp Tyr 
1               5                   10                  15      


Leu Pro Asn Asp Gly Asn His Trp Asn Arg Leu Asn Ser Asp Ala Ser 
            20                  25                  30          


Asn Leu Lys Ser Lys Gly Ile Thr Ala Val Trp Ile Pro Pro Ala Trp 
        35                  40                  45              


Lys Gly Ala Ser Gln Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 
    50                  55                  60                  


Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly 
65                  70                  75                  80  


Thr Arg Ser Gln Leu Gln Ala Ala Val Thr Ser Leu Lys Asn Asn Gly 
                85                  90                  95      


Ile Gln Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp 
            100                 105                 110         


Tyr Thr Glu Thr Val Thr Ala Val Glu Val Asn Pro Asn Asn Arg Asn 
        115                 120                 125             


Gln Glu Thr Ser Gly Glu Tyr Asn Ile Gln Ala Trp Thr Arg Phe Asp 
    130                 135                 140                 


Phe Pro Gly Arg Gly Asn Thr His Ser Ser Phe Lys Trp Arg Trp Tyr 
145                 150                 155                 160 


His Phe Asp Gly Val Asp Trp Asp Gln Ser Arg Arg Leu Asn Asn Arg 
                165                 170                 175     


Ile Tyr Lys Phe Asp Gly Lys Ala Trp Asp Trp Pro Val Ser Ser Glu 
            180                 185                 190         


Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Val Asp Phe Glu His 
        195                 200                 205             


Pro Glu Val Val Asn Glu Met Lys Lys Trp Gly Val Trp Tyr Thr Asn 
    210                 215                 220                 


Thr Leu Gly Leu Asp Gly Phe Arg Ile Asp Ala Val Lys His Ile Lys 
225                 230                 235                 240 


Tyr Ser Phe Thr Arg Asp Trp Ile Asn His Val Arg Ser Ala Thr Gly 
                245                 250                 255     


Lys Asn Met Phe Ala Val Ala Glu Phe Trp Lys Asn Asp Leu Gly Ala 
            260                 265                 270         


Ile Glu Asn Tyr Leu Gln Lys Thr Asn Trp Asn His Ser Val Phe Asp 
        275                 280                 285             


Val Pro Leu His Tyr Asn Leu Tyr Asn Ala Ser Lys Ser Gly Gly Asn 
    290                 295                 300                 


Tyr Asp Met Arg Asn Ile Phe Asn Gly Thr Val Val Gln Arg His Pro 
305                 310                 315                 320 


Ser His Ala Val Thr Phe Val Asp Asn His Asp Ser Gln Pro Glu Glu 
                325                 330                 335     


Ala Leu Glu Ser Phe Val Glu Glu Trp Phe Lys Pro Leu Ala Tyr Ala 
            340                 345                 350         


Leu Thr Leu Thr Arg Glu Gln Gly Tyr Pro Ser Val Phe Tyr Gly Asp 
        355                 360                 365             


Tyr Tyr Gly Ile Pro Thr His Gly Val Pro Ala Met Arg Ser Lys Ile 
    370                 375                 380                 


Asp Pro Ile Leu Glu Ala Arg Gln Lys Tyr Ala Tyr Gly Lys Gln Asn 
385                 390                 395                 400 


Asp Tyr Leu Asp His His Asn Ile Ile Gly Trp Thr Arg Glu Gly Asn 
                405                 410                 415     


Thr Ala His Pro Asn Ser Gly Leu Ala Thr Leu Ile Ser Asp Gly Pro 
            420                 425                 430         


Gly Gly Ser Lys Trp Met Phe Val Gly Arg Asn Lys Ala Gly Gln Val 
        435                 440                 445             


Trp Ser Asp Ile Thr Gly Asn Arg Thr Gly Thr Val Thr Ile Asn Ala 
    450                 455                 460                 


Asp Gly Trp Gly Asn Phe Ser Val Asn Gly Gly Ser Val Ser Ile Trp 
465                 470                 475                 480 


Val Asn Lys 
            


<210>  4
<211>  483
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of Amy707 alpha-amylase

<400>  4

His His Asn Gly Thr Asn Gly Thr Met Met Gln Tyr Phe Glu Trp Tyr 
1               5                   10                  15      


Leu Pro Asn Asp Gly Asn His Trp Asn Arg Leu Asn Ser Asp Ala Ser 
            20                  25                  30          


Asn Leu Lys Ser Lys Gly Ile Thr Ala Val Trp Ile Pro Pro Ala Trp 
        35                  40                  45              


Lys Gly Thr Ser Gln Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 
    50                  55                  60                  


Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly 
65                  70                  75                  80  


Thr Arg Ser Gln Leu Gln Ala Ala Val Thr Ser Leu Lys Asn Asn Gly 
                85                  90                  95      


Ile Gln Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp 
            100                 105                 110         


Ala Thr Glu Met Val Arg Ala Val Glu Val Asn Pro Asn Asn Arg Asn 
        115                 120                 125             


Gln Glu Val Thr Gly Glu Tyr Thr Ile Glu Ala Trp Thr Arg Phe Asp 
    130                 135                 140                 


Phe Pro Gly Arg Gly Asn Thr His Ser Asn Phe Lys Trp Arg Trp Tyr 
145                 150                 155                 160 


His Phe Asp Gly Val Asp Trp Asp Gln Ser Arg Arg Leu Asn Asn Arg 
                165                 170                 175     


Ile Tyr Lys Phe Asp Gly Lys Ala Trp Asp Trp Pro Val Ser Ser Glu 
            180                 185                 190         


Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Leu Asp Phe Asp His 
        195                 200                 205             


Pro Asp Val Ala Asn Glu Met Lys Asn Trp Gly Thr Trp Tyr Ala Asn 
    210                 215                 220                 


Glu Leu Asn Leu Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile Asp 
225                 230                 235                 240 


His Glu Tyr Leu Arg Asp Trp Val Asn His Val Arg Gln Gln Thr Gly 
                245                 250                 255     


Lys Asn Met Phe Ala Val Ala Glu Phe Trp Lys Asn Asp Leu Gly Ala 
            260                 265                 270         


Ile Glu Asn Tyr Leu Gln Lys Thr Asn Trp Asn Gln Ser Val Phe Asp 
        275                 280                 285             


Ala Pro Leu His Tyr Asn Leu Tyr Asn Ala Ser Lys Ser Gly Gly Asn 
    290                 295                 300                 


Tyr Asp Met Arg Asn Ile Phe Asn Gly Thr Val Val Gln Arg His Pro 
305                 310                 315                 320 


Ser His Ala Val Thr Phe Val Asp Asn His Asp Ser Gln Pro Glu Glu 
                325                 330                 335     


Ala Leu Glu Ser Phe Val Glu Glu Trp Phe Lys Pro Leu Ala Tyr Ala 
            340                 345                 350         


Leu Thr Leu Thr Arg Glu Gln Gly Tyr Pro Ser Val Phe Tyr Gly Asp 
        355                 360                 365             


Tyr Tyr Gly Ile Pro Thr His Gly Val Pro Ala Met Arg Ser Lys Ile 
    370                 375                 380                 


Asp Pro Ile Leu Glu Ala Arg Gln Lys Tyr Ala Tyr Gly Lys Gln Asn 
385                 390                 395                 400 


Asp Tyr Leu Asp His His Asn Ile Ile Gly Trp Thr Arg Glu Gly Asp 
                405                 410                 415     


Ser Thr Lys Ala Asn Ser Gly Leu Ala Thr Ile Met Ser Asp Gly Pro 
            420                 425                 430         


Gly Gly Ser Lys Trp Met Asn Val Gly Lys Gln Asn Ala Gly Glu Val 
        435                 440                 445             


Trp Tyr Asp Ile Thr Gly Asn Arg Thr Gly Thr Val Thr Ile Asn Ser 
    450                 455                 460                 


Asp Gly Trp Gly Gln Phe Phe Val Asn Gly Gly Ser Val Ser Ile Trp 
465                 470                 475                 480 


Val Asn Lys 
            


<210>  5
<211>  483
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of Amy707 alpha-amylase

<400>  5

His His Asn Gly Thr Asn Gly Thr Leu Met Gln Tyr Phe Glu Trp Tyr 
1               5                   10                  15      


Ala Pro Asn Asp Gly Gln His Trp Asn Arg Leu Arg Ser Asp Ala Glu 
            20                  25                  30          


Asn Leu Ala Gln Lys Gly Ile Thr Ala Val Trp Ile Pro Pro Ala Trp 
        35                  40                  45              


Lys Gly Ala Ser Gln Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 
    50                  55                  60                  


Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly 
65                  70                  75                  80  


Thr Lys Ala Gln Leu Lys Ser Ala Val Thr Ser Leu Lys Asn Asn Gly 
                85                  90                  95      


Ile Gln Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp 
            100                 105                 110         


Ala Thr Glu Thr Val Thr Ala Val Glu Val Asn Pro Ser Asn Arg Asn 
        115                 120                 125             


Gln Glu Thr Ser Gly Glu Tyr Asn Ile Gln Ala Trp Thr Arg Phe Asp 
    130                 135                 140                 


Phe Pro Gly Arg Gly Asn Thr His Ser Ser Phe Lys Trp Arg Trp Tyr 
145                 150                 155                 160 


His Phe Asp Gly Thr Asp Trp Asp Gln Ser Arg Arg Leu Asn Asn Arg 
                165                 170                 175     


Ile Tyr Lys Phe Asp Gly Lys Ala Trp Asp Trp Pro Val Ser Ser Glu 
            180                 185                 190         


Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Met Asp His 
        195                 200                 205             


Pro Glu Val Val Asn Glu Leu Arg Asn Trp Gly Val Trp Tyr Thr Asn 
    210                 215                 220                 


Thr Leu Gly Leu Asp Gly Phe Arg Ile Asp Ala Val Lys His Ile Lys 
225                 230                 235                 240 


Tyr Ser Phe Thr Arg Asp Trp Ile Asn His Val Arg Ser Ala Thr Gly 
                245                 250                 255     


Lys Asn Met Phe Ala Val Ala Glu Phe Trp Lys Asn Asp Leu Gly Ala 
            260                 265                 270         


Ile Glu Asn Tyr Leu Gln Lys Thr Asn Trp Asn His Ser Val Phe Asp 
        275                 280                 285             


Val Pro Leu His Tyr Asn Leu Tyr Asn Ala Ser Lys Ser Gly Gly Asn 
    290                 295                 300                 


Tyr Asp Met Arg Asn Ile Phe Asn Gly Thr Val Val Gln Arg His Pro 
305                 310                 315                 320 


Ser His Ala Val Thr Phe Val Asp Asn His Asp Ser Gln Pro Glu Glu 
                325                 330                 335     


Ala Leu Glu Ser Phe Val Glu Glu Trp Phe Lys Pro Leu Ala Tyr Ala 
            340                 345                 350         


Leu Thr Leu Thr Arg Glu Gln Gly Tyr Pro Ser Val Phe Tyr Gly Asp 
        355                 360                 365             


Tyr Tyr Gly Ile Pro Thr His Gly Val Pro Ala Met Arg Ser Lys Ile 
    370                 375                 380                 


Asp Pro Ile Leu Glu Ala Arg Gln Lys Tyr Ala Tyr Gly Lys Gln Asn 
385                 390                 395                 400 


Asp Tyr Leu Asp His His Asn Ile Ile Gly Trp Thr Arg Glu Gly Asp 
                405                 410                 415     


Ser Thr Lys Ala Asn Ser Gly Leu Ala Thr Ile Met Ser Asp Gly Pro 
            420                 425                 430         


Gly Gly Ser Lys Trp Met Asn Val Gly Lys Asn Asn Ala Gly Gln Val 
        435                 440                 445             


Trp Ser Asp Ile Thr Gly Asn Arg Thr Gly Thr Val Thr Ile Asn Ala 
    450                 455                 460                 


Asp Gly Trp Gly Gln Phe Phe Val Asn Gly Gly Ser Val Ser Ile Trp 
465                 470                 475                 480 


Val Asn Lys 
            


<210>  6
<211>  482
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of Amy707 alpha-amylase

<400>  6

Ala Thr Val Asn Asn Gly Thr Leu Met Gln Tyr Phe Glu Trp Tyr Leu 
1               5                   10                  15      


Pro Asn Asp Gly Gln His Trp Asn Arg Leu Asn Ser Asp Ala Ser Asn 
            20                  25                  30          


Leu Lys Ser Lys Gly Ile Thr Ala Val Trp Ile Pro Pro Ala Tyr Lys 
        35                  40                  45              


Gly Thr Thr Gln Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr Asp 
    50                  55                  60                  


Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr 
65                  70                  75                  80  


Arg Ser Gln Leu Gln Ala Ala Val Thr Ser Leu Lys Asn Asn Gly Ile 
                85                  90                  95      


Gln Val Tyr Gly Asp Ala Val Met Asn His Lys Gly Gly Ala Asp Ala 
            100                 105                 110         


Thr Glu Met Val Arg Ala Val Glu Val Asn Pro Ser Asn Arg Asn Gln 
        115                 120                 125             


Glu Thr Ser Gly Glu Tyr Asn Ile Gln Ala Trp Thr Gly Phe Asn Phe 
    130                 135                 140                 


Pro Gly Arg Gly Asn Thr His Ser Ser Phe Lys Trp Arg Trp Tyr His 
145                 150                 155                 160 


Phe Asp Gly Val Asp Trp Asp Gln Ser Arg Arg Leu Asn Asn Arg Ile 
                165                 170                 175     


Tyr Lys Phe Asp Gly Lys Ala Trp Asp Trp Pro Val Ser Ser Glu Asn 
            180                 185                 190         


Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Leu Asp Phe Asp His Pro 
        195                 200                 205             


Asp Val Val Asn Glu Met Lys Glu Trp Gly Val Trp Tyr Ala Asn Thr 
    210                 215                 220                 


Leu Gly Leu Asp Gly Phe Arg Ile Asp Ala Val Lys His Ile Lys Tyr 
225                 230                 235                 240 


Ser Phe Thr Arg Asp Trp Ile Asn His Val Arg Ser Ala Thr Gly Lys 
                245                 250                 255     


Asn Met Phe Ala Val Ala Glu Phe Trp Lys Asn Asp Leu Gly Ala Ile 
            260                 265                 270         


Glu Asn Tyr Leu Gln Lys Thr Asn Trp Asn His Ser Val Phe Asp Val 
        275                 280                 285             


Pro Leu His Tyr Asn Leu Tyr Asn Ala Ser Lys Ser Gly Gly Asn Tyr 
    290                 295                 300                 


Asp Met Arg Asn Ile Phe Asn Gly Thr Val Val Gln Arg His Pro Ser 
305                 310                 315                 320 


His Ala Val Thr Phe Val Asp Asn His Asp Ser Gln Pro Glu Glu Ala 
                325                 330                 335     


Leu Glu Ser Phe Val Glu Glu Trp Phe Lys Pro Leu Ala Tyr Ala Leu 
            340                 345                 350         


Thr Leu Thr Arg Glu Gln Gly Tyr Pro Ser Val Phe Tyr Gly Asp Tyr 
        355                 360                 365             


Tyr Gly Ile Pro Thr His Gly Val Pro Ala Met Arg Ser Lys Ile Asp 
    370                 375                 380                 


Pro Ile Leu Glu Ala Arg Gln Lys Tyr Ala Tyr Gly Lys Gln Asn Asp 
385                 390                 395                 400 


Tyr Leu Asp His His Asn Ile Ile Gly Trp Thr Arg Glu Gly Asn Thr 
                405                 410                 415     


Ala His Pro Asn Ser Gly Leu Ala Thr Ile Met Ser Asp Gly Pro Gly 
            420                 425                 430         


Gly Ser Lys Trp Met Phe Val Gly Arg Asn Lys Ala Gly Gln Val Trp 
        435                 440                 445             


Ser Asp Ile Thr Gly Asn Arg Thr Gly Thr Val Thr Ile Asn Ala Asp 
    450                 455                 460                 


Gly Trp Gly Asn Phe Ser Val Asn Gly Gly Ser Val Ser Ile Trp Val 
465                 470                 475                 480 


Asn Lys 
        


<210>  7
<211>  485
<212>  PRT
<213>  Bacillus sp. AA560

<400>  7

His His Asn Gly Thr Asn Gly Thr Met Met Gln Tyr Phe Glu Trp Tyr 
1               5                   10                  15      


Leu Pro Asn Asp Gly Asn His Trp Asn Arg Leu Arg Ser Asp Ala Ser 
            20                  25                  30          


Asn Leu Lys Asp Lys Gly Ile Ser Ala Val Trp Ile Pro Pro Ala Trp 
        35                  40                  45              


Lys Gly Ala Ser Gln Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 
    50                  55                  60                  


Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr Ile Arg Thr Lys Tyr Gly 
65                  70                  75                  80  


Thr Arg Asn Gln Leu Gln Ala Ala Val Asn Ala Leu Lys Ser Asn Gly 
                85                  90                  95      


Ile Gln Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp 
            100                 105                 110         


Ala Thr Glu Met Val Arg Ala Val Glu Val Asn Pro Asn Asn Arg Asn 
        115                 120                 125             


Gln Glu Val Ser Gly Glu Tyr Thr Ile Glu Ala Trp Thr Lys Phe Asp 
    130                 135                 140                 


Phe Pro Gly Arg Gly Asn Thr His Ser Asn Phe Lys Trp Arg Trp Tyr 
145                 150                 155                 160 


His Phe Asp Gly Val Asp Trp Asp Gln Ser Arg Lys Leu Asn Asn Arg 
                165                 170                 175     


Ile Tyr Lys Phe Arg Gly Asp Gly Lys Gly Trp Asp Trp Glu Val Asp 
            180                 185                 190         


Thr Glu Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Met 
        195                 200                 205             


Asp His Pro Glu Val Val Asn Glu Leu Arg Asn Trp Gly Val Trp Tyr 
    210                 215                 220                 


Thr Asn Thr Leu Gly Leu Asp Gly Phe Arg Ile Asp Ala Val Lys His 
225                 230                 235                 240 


Ile Lys Tyr Ser Phe Thr Arg Asp Trp Ile Asn His Val Arg Ser Ala 
                245                 250                 255     


Thr Gly Lys Asn Met Phe Ala Val Ala Glu Phe Trp Lys Asn Asp Leu 
            260                 265                 270         


Gly Ala Ile Glu Asn Tyr Leu Asn Lys Thr Asn Trp Asn His Ser Val 
        275                 280                 285             


Phe Asp Val Pro Leu His Tyr Asn Leu Tyr Asn Ala Ser Lys Ser Gly 
    290                 295                 300                 


Gly Asn Tyr Asp Met Arg Gln Ile Phe Asn Gly Thr Val Val Gln Arg 
305                 310                 315                 320 


His Pro Met His Ala Val Thr Phe Val Asp Asn His Asp Ser Gln Pro 
                325                 330                 335     


Glu Glu Ala Leu Glu Ser Phe Val Glu Glu Trp Phe Lys Pro Leu Ala 
            340                 345                 350         


Tyr Ala Leu Thr Leu Thr Arg Glu Gln Gly Tyr Pro Ser Val Phe Tyr 
        355                 360                 365             


Gly Asp Tyr Tyr Gly Ile Pro Thr His Gly Val Pro Ala Met Lys Ser 
    370                 375                 380                 


Lys Ile Asp Pro Ile Leu Glu Ala Arg Gln Lys Tyr Ala Tyr Gly Arg 
385                 390                 395                 400 


Gln Asn Asp Tyr Leu Asp His His Asn Ile Ile Gly Trp Thr Arg Glu 
                405                 410                 415     


Gly Asn Thr Ala His Pro Asn Ser Gly Leu Ala Thr Ile Met Ser Asp 
            420                 425                 430         


Gly Ala Gly Gly Asn Lys Trp Met Phe Val Gly Arg Asn Lys Ala Gly 
        435                 440                 445             


Gln Val Trp Thr Asp Ile Thr Gly Asn Arg Ala Gly Thr Val Thr Ile 
    450                 455                 460                 


Asn Ala Asp Gly Trp Gly Asn Phe Ser Val Asn Gly Gly Ser Val Ser 
465                 470                 475                 480 


Ile Trp Val Asn Lys 
                485 


<210>  8
<211>  1452
<212>  DNA
<213>  Artificial

<220>
<223>  Coding sequence for Amy707 variant

<400>  8
catcataacg gtacaaacgg aacaatgatg cagtatttcg agtggtatct acctaacgac       60

ggcaatcatt ggaacagact gaactcagat gccagtaact tgaaatctaa aggtattaca      120

gcagtatgga ttccgccggc ttataagggc acgtctcaaa atgatgtggg gtacggcgca      180

tacgatctgt atgatttagg cgagtttaat caaaaaggta cagtcagaac gaagtatggc      240

acaagaagtc aacttcaagc cgctgttacc tcgctgaaaa ataatggaat ccaagtgtat      300

ggtgatgtcg tgatgaatca taaaggaggt gctgatgcta cagaaatggt aagggcggtg      360

gaggtgaatc cgtctaatag aaatcaggaa attagcggcg attatacaat tgaagcatgg      420

acaaaattcg attttccggg ccgaggaaat acgcattcta gcttcaaatg gaggtggtac      480

cattttgatg gagtggattg ggaccagagc agaagactca acaatagaat ctacaaattc      540

catggaaagg cgtgggactg gccggtttct tcagaaaacg gaaattatga ctatctgatg      600

tacgcggaca ttgatatgga ccatccggat gtcgtgaatg aattgagaaa ttggggcgtc      660

tggtatgcca acacattagg gttagatgga ttcagaattg acgcggtcaa acatattaaa      720

tatagcttta ccagagactg gattaatcac gtaagatcag cgacgggaaa aaatatgttt      780

gcggttgccg aattttggaa aaatgattta ggggccattg aaaactacct gcaaaagaca      840

aattggaacc attctgtttt tgatgtgccg ttgcattaca acctttacaa tgcctcaaaa      900

agcggtggaa attacgatat gagaaatatt tttaatggca cggtagtcca acgccatccg      960

agtcatgctg ttacgtttgt tgataatcat gactcacagc ctgaagaagc actggaatca     1020

tttgtcgaag agtggtttaa accgttagcc tatgcgctta ctctcacgag agaacaaggc     1080

tatccttctg tattttatgg agattattac ggtattccaa cacatggggt ccctgctatg     1140

agatctaaaa tcgatcctat tcttgaggct agacaaaagt atgcttatgg aaaacagaat     1200

gactatttag atcaccataa tatcattggc tggacgagag aagggaacac agcgcacccc     1260

aatagcggtc tggccacaat catgtcagat ggggcgggcg gttcaaaatg gatgaatgtt     1320

ggcaaaaata atgcgggtga agtttggtat gatattacag ggaatagaac aggtacagtc     1380

acgattaatg ccgatggctg gggccaattt catgtcaatg ggggctcagt ttcaatatgg     1440

gtgaacaaat ga                                                         1452


<210>  9
<211>  1452
<212>  DNA
<213>  Artificial

<220>
<223>  Coding sequence for Amy707 variant

<400>  9
catcataacg gtacgaacgg gacaatgatg caatactttg aatggtatct acctaatgac       60

ggaaatcatt ggaatcgatt aaactctgat gcgagtaacc ttaaaagcaa agggattaca      120

gcggtgtgga ttcctccagc atggaagggc gcttctcaaa atgacgtagg atacggagcc      180

tatgacctgt atgatctggg agaatttaat caaaaaggta ccgtccgtac aaaatatgga      240

acacgtagtc agttacaagc tgcggtaacc tccttaaaaa ataatggaat tcaagtatat      300

ggtgacgttg ttatgaatca caaaggtggc gcagactata ctgaaacagt aacggccgtt      360

gaagtgaatc ccaataaccg taaccaagaa acatctggtg aatataatat tcaagcttgg      420

actagatttg attttccagg gcgaggaaat actcattcta gctttaaatg gagatggtat      480

cattttgatg gtgtggattg ggatcagtca cgtagactga acaatcgcat ctataaattt      540

gatggcaaag cttgggattg gccagttagt tctgaaaatg gtaattatga ttatttgatg      600

tacgctgatg ttgattttga acacccagaa gtagtaaatg aaatgaaaaa gtggggtgtt      660

tggtacacaa acacattagg actcgatgga tttagaatag atgcggttaa acatataaag      720

tatagcttta cgcgcgattg gattaatcac gttagaagtg caacaggtaa aaatatgttt      780

gcggttgctg agttttggaa gaatgattta ggtgcaattg aaaactatct gcagaaaaca      840

aactggaacc attcagtctt tgatgtgccg ttacattata atctgtataa tgcatcaaaa      900

agcggaggga actatgatat gcgaaacata tttaatggaa cggttgttca acgacatcca      960

agtcatgctg taacatttgt tgataatcat gattcgcagc ctgaagaagc attagaatct     1020

tttgttgaag aatggtttaa accattagcg tatgcgctta cattaacgcg tgaacaagga     1080

tacccttctg tattttacgg agattattat gggattccaa cacatggagt gccagcaatg     1140

agatcaaaaa tcgatccgat tttagaagca cgtcaaaagt atgcatacgg aaaacaaaat     1200

gattacttag accatcataa tatcattggt tggacgcgtg aagggaatac agcacacccc     1260

aattcaggtc tagctacctt aatttctgat ggaccaggtg gaagtaagtg gatgtttgtt     1320

gggcgtaata aggctggtca agtatggagt gatattacag gaaaccgtac aggtacggtt     1380

acaatcaatg cagacggttg gggcaatttc tctgtgaatg gagggtcagt ttctatttgg     1440

gtcaacaaat aa                                                         1452


<210>  10
<211>  1452
<212>  DNA
<213>  Artificial

<220>
<223>  Coding sequence for Amy707 variant

<400>  10
catcataacg gtacgaacgg gacaatgatg caatactttg aatggtatct acctaatgac       60

ggaaatcatt ggaatcgatt aaactctgat gcgagtaacc ttaaaagcaa agggattaca      120

gcggtgtgga ttcctccagc atggaagggc acatctcaaa atgacgtagg atacggagcc      180

tatgacctgt atgatctggg agaatttaat caaaaaggta ccgtccgtac aaaatatgga      240

acacgtagtc agttacaagc tgcggtaacc tccttaaaaa ataatggaat tcaagtatat      300

ggtgacgttg ttatgaatca caaaggtggc gcagacgcta ctgaaatggt aagggccgtt      360

gaagtgaatc ccaataaccg taaccaagaa gtgactggtg aatataccat tgaagcttgg      420

actagatttg attttccagg gcgaggaaat actcattcta attttaaatg gagatggtat      480

cattttgatg gtgtggattg ggatcagtca cgtagactga acaatcgcat ctataaattt      540

gatggcaaag cttgggattg gccagtttca tctgaaaatg gtaattatga ttatttgatg      600

tacgctgatt tagattttga tcacccagat gtagcaaatg aaatgaaaaa ttggggtaca      660

tggtacgcaa acgaattaaa tctcgatgga tttagattag atgcggttaa acatatagat      720

catgaatatt tacgcgattg ggttaatcac gttagacaac agacaggtaa aaatatgttt      780

gcggttgctg agttttggaa gaatgattta ggtgcaattg aaaactatct gcagaaaaca      840

aactggaacc aatcagtctt tgatgctccg ttacattata atctgtataa tgcatcaaaa      900

agcggaggga actatgatat gcgaaacata tttaatggaa cggttgttca acgacatcca      960

agtcatgctg taacatttgt tgataatcat gattcgcagc ctgaagaagc attagaatct     1020

tttgttgaag aatggtttaa accattagcg tatgcgctta cattaacgcg tgaacaagga     1080

tacccttctg tattttacgg agattattat gggattccaa cacatggagt gccagcaatg     1140

agatcaaaaa tcgatccgat tttagaagca cgtcaaaagt atgcatacgg aaaacaaaat     1200

gattacttag accatcataa tatcattggt tggacgcgtg aaggggatag tacaaaagca     1260

aattcaggtc tagctaccat catgtctgat ggaccaggtg gaagtaagtg gatgaatgtt     1320

gggaaacaaa atgctggtga agtatggtat gatattacag gaaaccgtac aggtacggtt     1380

acaatcaatt ctgacggttg gggccaattc tttgtgaatg gagggtcagt ttctatttgg     1440

gtcaacaaat aa                                                         1452


<210>  11
<211>  1452
<212>  DNA
<213>  Artificial

<220>
<223>  Coding sequence for Amy707 variant

<400>  11
catcataacg gtacgaacgg gacattaatg caatactttg aatggtatgc acctaatgac       60

ggacaacatt ggaatcgatt aagatctgat gcggaaaacc ttgcgcaaaa agggattaca      120

gcggtgtgga ttcctccagc atggaagggc gcttctcaaa atgacgtagg atacggagcc      180

tatgacctgt atgatctggg agaatttaat caaaaaggta ccgtccgtac aaaatatgga      240

acaaaagcgc agttaaaaag tgcggtaacc tccttaaaaa ataatggaat tcaagtatat      300

ggtgacgttg ttatgaatca caaaggtggc gcagacgcta ctgaaacagt aacggccgtt      360

gaagtgaatc cctctaaccg taaccaagaa acatcaggtg aatataatat tcaagcttgg      420

actagatttg attttccagg gcgaggaaat actcattcta gctttaaatg gagatggtat      480

cattttgatg gtacagattg ggatcagtca cgtagactga acaatcgcat ctataaattt      540

gatggcaaag cttgggattg gccagttagt tctgaaaatg gtaattatga ttatttgatg      600

tacgctgata ttgatatgga tcacccagaa gtagtaaatg aattaagaaa ttggggtgtt      660

tggtacacaa acacattagg actcgatgga tttagaatag atgcggttaa acatataaag      720

tatagcttta cgcgcgattg gattaatcac gttagaagtg caacaggtaa aaatatgttt      780

gcggttgctg agttttggaa gaatgattta ggtgcaattg aaaactatct gcagaaaaca      840

aactggaacc attcagtctt tgatgtgccg ttacattata atctgtataa tgcatcaaaa      900

agcggaggga actatgatat gcgaaacata tttaatggaa cggttgttca acgacatcca      960

agtcatgctg taacatttgt tgataatcat gattcgcagc ctgaagaagc attagaatct     1020

tttgttgaag aatggtttaa accattagcg tatgcgctta cattaacgcg tgaacaagga     1080

tacccttctg tattttacgg agattattat gggattccaa cacatggagt gccagcaatg     1140

agatcaaaaa tcgatccgat tttagaagca cgtcaaaagt atgcatacgg aaaacaaaat     1200

gattacttag accatcataa tatcattggt tggacgcgtg aaggggattc tacaaaagca     1260

aattcaggtc tagctaccat catgtctgat ggaccaggtg gaagtaagtg gatgaatgtt     1320

gggaaaaata atgctggtca agtatggagt gatattacag gaaaccgtac aggtacggtt     1380

acaatcaatg cagacggttg gggccaattc tttgtgaatg gagggtcagt ttctatttgg     1440

gtcaacaaat aa                                                         1452


<210>  12
<211>  1449
<212>  DNA
<213>  Artificial

<220>
<223>  Coding sequence for Amy707 variant

<400>  12
gcaacagtga ataacgggac attaatgcaa tactttgaat ggtatctacc taatgacgga       60

caacattgga atcgattaaa ctctgatgcg agtaacctta aaagcaaagg gattacagcg      120

gtgtggattc ctccagcata taagggcaca acgcaaaatg acgtaggata cggagcctat      180

gacctgtatg atctgggaga atttaatcaa aaaggtaccg tccgtacaaa atatggaaca      240

cgtagtcagt tacaagctgc ggtaacctcc ttaaaaaata atggaattca agtatatggt      300

gacgcggtta tgaatcacaa aggtggcgca gacgctactg aaatggtaag ggccgttgaa      360

gtgaatccct ctaaccgtaa ccaagaaaca tctggtgaat ataatattca agcttggact      420

ggatttaatt ttccagggcg aggaaatact cattctagct ttaaatggag atggtatcat      480

tttgatggtg tggattggga tcagtcacgt agactgaaca atcgcatcta taaatttgat      540

ggcaaagctt gggattggcc agttagttct gaaaatggta attatgatta tttgatgtac      600

gctgatttag attttgatca cccagatgta gtaaatgaaa tgaaggaatg gggtgtttgg      660

tacgcaaaca cattaggact cgatggattt agaatagatg cggttaaaca tataaagtat      720

agctttacgc gcgattggat taatcacgtt agaagtgcaa caggtaaaaa tatgtttgcg      780

gttgctgagt tttggaagaa tgatttaggt gcaattgaaa actatctgca gaaaacaaac      840

tggaaccatt cagtctttga tgtgccgtta cattataatc tgtataatgc atcaaaaagc      900

ggagggaact atgatatgcg aaacatattt aatggaacgg ttgttcaacg acatccaagt      960

catgctgtaa catttgttga taatcatgat tcgcagcctg aagaagcatt agaatctttt     1020

gttgaagaat ggtttaaacc attagcgtat gcgcttacat taacgcgtga acaaggatac     1080

ccttctgtat tttacggaga ttattatggg attccaacac atggagtgcc agcaatgaga     1140

tcaaaaatcg atccgatttt agaagcacgt caaaagtatg catacggaaa acaaaatgat     1200

tacttagacc atcataatat cattggttgg acgcgtgaag ggaatacagc acaccccaat     1260

tcaggtctag ctaccatcat gtctgatgga ccaggtggaa gtaagtggat gtttgttggg     1320

cgtaataagg ctggtcaagt atggagtgat attacaggaa accgtacagg tacggttaca     1380

atcaatgcag acggttgggg caatttctct gtgaatggag ggtcagtttc tatttgggtc     1440

aacaaataa                                                             1449


