                         SEQUENCE LISTING

<110>  INDIAN INSTITUTE OF TECHNOLOGY DELHI
 
<120>  A RECOMBINANT VECTOR COMPRISING A FUSION DNA FOR CELL SURFACE 
       DISPLAY, AND USES THEREOF

<130>  PD025163IN-SC

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  84
<212>  DNA
<213>  Artificial sequence

<220>
<223>  N-terminal region BclB gene

<400>  1
ttgaaacaga atgacaaatt atggttggat aaaggcataa taggcccaga aaatattgga       60

cctaccttcc cggttcttcc ccca                                              84


<210>  2
<211>  28
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal domain of BclB protein

<400>  2

Leu Lys Gln Asn Asp Lys Leu Trp Leu Asp Lys Gly Ile Ile Gly Pro 
1               5                   10                  15      


Glu Asn Ile Gly Pro Thr Phe Pro Val Leu Pro Pro 
            20                  25              


<210>  3
<211>  1251
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DszC DNA

<400>  3
atgactctgt ccgttgaaaa gcagcacgtt cgacccggcg atgccgacaa cgatcccgtc       60

gcagtcgccc gtgggctcgc cgagaagtgg cgagccaccg ccgtcgagcg tgatcgcgcc      120

gggggttcgg caacagtcga gcgcgaagac ctgcgcgcga gcggcctgct gtcgctgctg      180

atcccacgcc agtacggcgg gtggggcgcg gactggccca ccgccatcga agtggtccga      240

gaaatcgccg cagccgatgg atctttggga cacctgctcg gctaccacct cagcagcgcc      300

ccgatgatcg aactgttcgg ctcgcaggaa caagaacaac ggctataccg gcaaatcgcg      360

cagaacgact ggtggaccgg gaacgcctcc agcgaaaaca acagccacgt gctggactgg      420

aaggtcagcg ccagcccgac cgaggacggc ggttatctgc tcaacggcac caagcacttc      480

tgcagcggcg ccaagggttc ggacctgttg ctggtcttcg gcgtcatcca ggacgattct      540

ccgcagcagg gcgcgatcat cgcggccgtc atcccgacat cccggcacgg cgttcaggtc      600

aacgacgact gggccgccat cggcatgcgg cagaccgaca gcggatccac cgatttccac      660

agcgtcaagg tcgagcctga cgaagtgctg ggtgagccca acgccttcat tgtcgccttc      720

atccagtccg agcgcggcag cctcttcgcg cccatagtgc aattgatctt cgccaacgtc      780

tatttaggga tcgcgcacgg cgcactcgac gccgcccggg agtacacccg cactcaggcc      840

aggccatgga caccggccgg ggttcaacag gcaaccgagg atccctacgt cctgcgcgcc      900

tatggtgaat tcaccatcgc attgcagggc gccgacgccg ctgcccgcga agcggcccat      960

ctgctgcaga cggtgtggga caagggcgat gcgctcaccc ccgaggaccg cggcgaactg     1020

atggtgaaga tctccggggt caaagcgttg gccacgaacg ccgccctcga cgtcaacagc     1080

ggaatcttcg aggtgatcgg agcgcgcgga acacatccca agtacggttt cgaccgcttc     1140

tggcgcaacg tacgcacgca caccctccac gatccggtgt cctacaagat cgccgacgtc     1200

ggcaaacaca ccctcaacgg gcagtacccg attcccggct tcacctccta g              1251


<210>  4
<211>  416
<212>  PRT
<213>  Artificial sequence

<220>
<223>  DszC protein

<400>  4

Met Thr Leu Ser Val Glu Lys Gln His Val Arg Pro Gly Asp Ala Asp 
1               5                   10                  15      


Asn Asp Pro Val Ala Val Ala Arg Gly Leu Ala Glu Lys Trp Arg Ala 
            20                  25                  30          


Thr Ala Val Glu Arg Asp Arg Ala Gly Gly Ser Ala Thr Val Glu Arg 
        35                  40                  45              


Glu Asp Leu Arg Ala Ser Gly Leu Leu Ser Leu Leu Ile Pro Arg Gln 
    50                  55                  60                  


Tyr Gly Gly Trp Gly Ala Asp Trp Pro Thr Ala Ile Glu Val Val Arg 
65                  70                  75                  80  


Glu Ile Ala Ala Ala Asp Gly Ser Leu Gly His Leu Leu Gly Tyr His 
                85                  90                  95      


Leu Ser Ser Ala Pro Met Ile Glu Leu Phe Gly Ser Gln Glu Gln Glu 
            100                 105                 110         


Gln Arg Leu Tyr Arg Gln Ile Ala Gln Asn Asp Trp Trp Thr Gly Asn 
        115                 120                 125             


Ala Ser Ser Glu Asn Asn Ser His Val Leu Asp Trp Lys Val Ser Ala 
    130                 135                 140                 


Ser Pro Thr Glu Asp Gly Gly Tyr Leu Leu Asn Gly Thr Lys His Phe 
145                 150                 155                 160 


Cys Ser Gly Ala Lys Gly Ser Asp Leu Leu Leu Val Phe Gly Val Ile 
                165                 170                 175     


Gln Asp Asp Ser Pro Gln Gln Gly Ala Ile Ile Ala Ala Val Ile Pro 
            180                 185                 190         


Thr Ser Arg His Gly Val Gln Val Asn Asp Asp Trp Ala Ala Ile Gly 
        195                 200                 205             


Met Arg Gln Thr Asp Ser Gly Ser Thr Asp Phe His Ser Val Lys Val 
    210                 215                 220                 


Glu Pro Asp Glu Val Leu Gly Glu Pro Asn Ala Phe Ile Val Ala Phe 
225                 230                 235                 240 


Ile Gln Ser Glu Arg Gly Ser Leu Phe Ala Pro Ile Val Gln Leu Ile 
                245                 250                 255     


Phe Ala Asn Val Tyr Leu Gly Ile Ala His Gly Ala Leu Asp Ala Ala 
            260                 265                 270         


Arg Glu Tyr Thr Arg Thr Gln Ala Arg Pro Trp Thr Pro Ala Gly Val 
        275                 280                 285             


Gln Gln Ala Thr Glu Asp Pro Tyr Val Leu Arg Ala Tyr Gly Glu Phe 
    290                 295                 300                 


Thr Ile Ala Leu Gln Gly Ala Asp Ala Ala Ala Arg Glu Ala Ala His 
305                 310                 315                 320 


Leu Leu Gln Thr Val Trp Asp Lys Gly Asp Ala Leu Thr Pro Glu Asp 
                325                 330                 335     


Arg Gly Glu Leu Met Val Lys Ile Ser Gly Val Lys Ala Leu Ala Thr 
            340                 345                 350         


Asn Ala Ala Leu Asp Val Asn Ser Gly Ile Phe Glu Val Ile Gly Ala 
        355                 360                 365             


Arg Gly Thr His Pro Lys Tyr Gly Phe Asp Arg Phe Trp Arg Asn Val 
    370                 375                 380                 


Arg Thr His Thr Leu His Asp Pro Val Ser Tyr Lys Ile Ala Asp Val 
385                 390                 395                 400 


Gly Lys His Thr Leu Asn Gly Gln Tyr Pro Ile Pro Gly Phe Thr Ser 
                405                 410                 415     


<210>  5
<211>  579
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DszD DNA

<400>  5
atgtctgaca agccgaatgc cgtttccagc cacaccaccc ccgacgtccc cgaagtagca       60

gcgacgcccg agttgtccac cggcatctgc gccggtgact accgtgcagc gcttcgccgc      120

caccccgcag gtgtcaccgt cgtgaccctc gattcgggta ccggcccggt gggtttcacc      180

gccacctcgt tctcgtccgt ctccctggag ccgccgctcg tctcgttcaa catcgcggag      240

acgtcgtcga gcatcaatgc gctcaaggca gccgagtcct tggtgatcca ccttctcggc      300

gaacatcagc aacatctggc ccagcgcttt gcgcgtagcg ccgatcagcg ttttgcagac      360

gagtcactgt gggcagtgct cgacaccggg gagccggttc tgcacggcac ccccagctgg      420

atgcgcgtca aggtcgacca gttgatccct gtcggcgacc acacgctggt catcggactc      480

gtcacgcggg ttcacgccga agaggacgac gaatccgctg ccgcaccgct gctctaccac      540

gagggcaagt actaccgccc gactccgtta ggtcaatag                             579


<210>  6
<211>  192
<212>  PRT
<213>  Artificial sequence

<220>
<223>  DszD protein

<400>  6

Met Ser Asp Lys Pro Asn Ala Val Ser Ser His Thr Thr Pro Asp Val 
1               5                   10                  15      


Pro Glu Val Ala Ala Thr Pro Glu Leu Ser Thr Gly Ile Cys Ala Gly 
            20                  25                  30          


Asp Tyr Arg Ala Ala Leu Arg Arg His Pro Ala Gly Val Thr Val Val 
        35                  40                  45              


Thr Leu Asp Ser Gly Thr Gly Pro Val Gly Phe Thr Ala Thr Ser Phe 
    50                  55                  60                  


Ser Ser Val Ser Leu Glu Pro Pro Leu Val Ser Phe Asn Ile Ala Glu 
65                  70                  75                  80  


Thr Ser Ser Ser Ile Asn Ala Leu Lys Ala Ala Glu Ser Leu Val Ile 
                85                  90                  95      


His Leu Leu Gly Glu His Gln Gln His Leu Ala Gln Arg Phe Ala Arg 
            100                 105                 110         


Ser Ala Asp Gln Arg Phe Ala Asp Glu Ser Leu Trp Ala Val Leu Asp 
        115                 120                 125             


Thr Gly Glu Pro Val Leu His Gly Thr Pro Ser Trp Met Arg Val Lys 
    130                 135                 140                 


Val Asp Gln Leu Ile Pro Val Gly Asp His Thr Leu Val Ile Gly Leu 
145                 150                 155                 160 


Val Thr Arg Val His Ala Glu Glu Asp Asp Glu Ser Ala Ala Ala Pro 
                165                 170                 175     


Leu Leu Tyr His Glu Gly Lys Tyr Tyr Arg Pro Thr Pro Leu Gly Gln 
            180                 185                 190         


<210>  7
<211>  1425
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DszA DNA

<400>  7
atggctcaac ggcgacaact gcatctggcc ggtttcttct cggctggcaa tgtgactcat       60

gcacatgggg cgtggcggca tacggacgcg tcgaatggtt ttttgaccgg gaagtactac      120

caacacatcg cccgcactct ggagcgcggc aagttcgatc tgttgttcct gcctgacgga      180

ttggccgtcg aggacagcta cggcgacgac ctgcgaaccg gtgtcggcct cggcgggcag      240

ggtgcagtcg ccttggagcc ggccagcgtg atagcgacca tggccgcggt caccgaacac      300

ctcggtctcg gggcaaccat ttcggcgacc tactatcccc cgtatcacgt tgctcgggtg      360

ttcgcgacgc tcgatcaatt gtcggggggc cgggtttcgt ggaacgtggt cacctcgctc      420

aacgacgctg aagcgcgcaa cttcggtatt gatcagcatc tggaacacga cgcccgttac      480

gaccgcgccg atgagttctt ggatgcggtc aagaaactct ggaacagctg ggatgaggat      540

gcgctcgtac tggacaaggc ggcaggcgtg ttcgccgatc ccacgaaggt tcactacgtg      600

gatcaccacg gggagtggct gaatgtgcgc gggcctctgc aggtgcctcg ttcacctcag      660

ggtgagccgg tgatcctgca ggccgggttg tcgccgcgag gtcggcgctt cgccgggcgg      720

tgggccgagg ccgttttcag tgtcgcaccc gatctgggat tgatgcaggc tacatatcac      780

gacatcaaag cccaggtgaa ggctgcgggg cgtgatccgg atcagacgaa gatcttcacc      840

gccgtgatgc cggtgttggg cgagaccgag gccgtggcgc aggaccggct ggaatacctg      900

aacagtctgg tgcatccgga ggtcggactt tcgacgctgt ctagtcatac cggcatcaac      960

ttggcggagt acccgttgga cacgccgatc acgacgatcc tgcgggatct gcaggatcgc     1020

aacgtgccga cgcaactgca catgttcgcg gccgcgatgc acgccgagga gctcactttg     1080

gcggagctgg gtcggcggta tggaaccaac gtgggcttcg ttccgcagtg ggccggcacg     1140

gccgagcaga tcgccgagga gttgatccgc cacttcgacg ccggcgccgc ggatggcttc     1200

attgtttctc cggcctttct gcctggcgcg tatgacgaat tcgtcgacca ggtggtgccg     1260

gttctgcagg accgcggcta cttccgcacc gaatacgagg gcaacaccct gcgcgaccac     1320

ctgggtctgc gcgaaccacg accgttagga caaccttcat ggcaggccgc ctcagccccg     1380

gaaaccccgg ttcagaactt gataccggca tcctcgacac actga                     1425


<210>  8
<211>  474
<212>  PRT
<213>  Artificial sequence

<220>
<223>  DszA protein

<400>  8

Met Ala Gln Arg Arg Gln Leu His Leu Ala Gly Phe Phe Ser Ala Gly 
1               5                   10                  15      


Asn Val Thr His Ala His Gly Ala Trp Arg His Thr Asp Ala Ser Asn 
            20                  25                  30          


Gly Phe Leu Thr Gly Lys Tyr Tyr Gln His Ile Ala Arg Thr Leu Glu 
        35                  40                  45              


Arg Gly Lys Phe Asp Leu Leu Phe Leu Pro Asp Gly Leu Ala Val Glu 
    50                  55                  60                  


Asp Ser Tyr Gly Asp Asp Leu Arg Thr Gly Val Gly Leu Gly Gly Gln 
65                  70                  75                  80  


Gly Ala Val Ala Leu Glu Pro Ala Ser Val Ile Ala Thr Met Ala Ala 
                85                  90                  95      


Val Thr Glu His Leu Gly Leu Gly Ala Thr Ile Ser Ala Thr Tyr Tyr 
            100                 105                 110         


Pro Pro Tyr His Val Ala Arg Val Phe Ala Thr Leu Asp Gln Leu Ser 
        115                 120                 125             


Gly Gly Arg Val Ser Trp Asn Val Val Thr Ser Leu Asn Asp Ala Glu 
    130                 135                 140                 


Ala Arg Asn Phe Gly Ile Asp Gln His Leu Glu His Asp Ala Arg Tyr 
145                 150                 155                 160 


Asp Arg Ala Asp Glu Phe Leu Asp Ala Val Lys Lys Leu Trp Asn Ser 
                165                 170                 175     


Trp Asp Glu Asp Ala Leu Val Leu Asp Lys Ala Ala Gly Val Phe Ala 
            180                 185                 190         


Asp Pro Thr Lys Val His Tyr Val Asp His His Gly Glu Trp Leu Asn 
        195                 200                 205             


Val Arg Gly Pro Leu Gln Val Pro Arg Ser Pro Gln Gly Glu Pro Val 
    210                 215                 220                 


Ile Leu Gln Ala Gly Leu Ser Pro Arg Gly Arg Arg Phe Ala Gly Arg 
225                 230                 235                 240 


Trp Ala Glu Ala Val Phe Ser Val Ala Pro Asp Leu Gly Leu Met Gln 
                245                 250                 255     


Ala Thr Tyr His Asp Ile Lys Ala Gln Val Lys Ala Ala Gly Arg Asp 
            260                 265                 270         


Pro Asp Gln Thr Lys Ile Phe Thr Ala Val Met Pro Val Leu Gly Glu 
        275                 280                 285             


Thr Glu Ala Val Ala Gln Asp Arg Leu Glu Tyr Leu Asn Ser Leu Val 
    290                 295                 300                 


His Pro Glu Val Gly Leu Ser Thr Leu Ser Ser His Thr Gly Ile Asn 
305                 310                 315                 320 


Leu Ala Glu Tyr Pro Leu Asp Thr Pro Ile Thr Thr Ile Leu Arg Asp 
                325                 330                 335     


Leu Gln Asp Arg Asn Val Pro Thr Gln Leu His Met Phe Ala Ala Ala 
            340                 345                 350         


Met His Ala Glu Glu Leu Thr Leu Ala Glu Leu Gly Arg Arg Tyr Gly 
        355                 360                 365             


Thr Asn Val Gly Phe Val Pro Gln Trp Ala Gly Thr Ala Glu Gln Ile 
    370                 375                 380                 


Ala Glu Glu Leu Ile Arg His Phe Asp Ala Gly Ala Ala Asp Gly Phe 
385                 390                 395                 400 


Ile Val Ser Pro Ala Phe Leu Pro Gly Ala Tyr Asp Glu Phe Val Asp 
                405                 410                 415     


Gln Val Val Pro Val Leu Gln Asp Arg Gly Tyr Phe Arg Thr Glu Tyr 
            420                 425                 430         


Glu Gly Asn Thr Leu Arg Asp His Leu Gly Leu Arg Glu Pro Arg Pro 
        435                 440                 445             


Leu Gly Gln Pro Ser Trp Gln Ala Ala Ser Ala Pro Glu Thr Pro Val 
    450                 455                 460                 


Gln Asn Leu Ile Pro Ala Ser Ser Thr His 
465                 470                 


<210>  9
<211>  1098
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DszB DNA

<400>  9
atggcaggcc gcctcagccc cggaaacccc ggttcagaac ttgataccgg catcctcgac       60

acactgacct acagcaactg cccgataccc aacgcgctgc tcacggcgtg ggaatcaggt      120

ttcctcgacg ccgccggcat cgaactcgac atcctcagcg gcaagcaggg aacggtccac      180

ttcacctacg accagcccgc ctacacccgc tatggcggtg agatcccgcc actgctcagc      240

gaggggttgc gggcacccgg acgcacgcgt ctactcggca tcaccccgat cctggggcgt      300

cagggcttct tcgtcggcga tcgcagcccg atcacagtgg ccgccgacct tgccggacgc      360

cgaataggag tgtcggcttc ggcgattcgc atcctgcgtg gcgaactggg ggactatctc      420

cagctggatc cctggcggca gacgctggta gcgctgggct cgtgggaggc gcgcgcgttg      480

ttgcacaccc tcgagcacgg tgagctcgat gtggacgacg tcgagctggt gccgatcaac      540

agtcctggcg tcgatgtccc cgccgagcag ttggaagacg ccgcgaccct caagggtgcg      600

gacctgtttc ccgatgtggc cgcaggccag gccgcggtgc tggaccgcgg tgaggttgac      660

gccctgttca gttggttgcc ctgggctgcg gagttggaag gcaccggcgc ccgcccggtg      720

gtggatctcg gcctcgatga gcgcaatgcc tatgccagcg tgtggacggt cagcagcgaa      780

ctggtcgttg accggcctga cctcgttcaa cgactggtcg acgcagtcgt cgacgccggg      840

ttgtgggcac gcgaccacgg cgatgcggtg acccgcctgc acgccgcgaa cctgggcgta      900

tcccccgacg cagtcggcca cggattcggc gccgacttcc agcagcgttt ggttccgcgc      960

ctggatcccg acgcggtcgc cctgctggat cgcacacagc aattcctgct cagcaaccag     1020

ttgctgcagg agcccgtcgc cctcgatcag tgggcggctc cggaatttct gaacaccagc     1080

ctcaaccgtc accgatag                                                   1098


<210>  10
<211>  365
<212>  PRT
<213>  Artificial sequence

<220>
<223>  DszB protein

<400>  10

Met Ala Gly Arg Leu Ser Pro Gly Asn Pro Gly Ser Glu Leu Asp Thr 
1               5                   10                  15      


Gly Ile Leu Asp Thr Leu Thr Tyr Ser Asn Cys Pro Ile Pro Asn Ala 
            20                  25                  30          


Leu Leu Thr Ala Trp Glu Ser Gly Phe Leu Asp Ala Ala Gly Ile Glu 
        35                  40                  45              


Leu Asp Ile Leu Ser Gly Lys Gln Gly Thr Val His Phe Thr Tyr Asp 
    50                  55                  60                  


Gln Pro Ala Tyr Thr Arg Tyr Gly Gly Glu Ile Pro Pro Leu Leu Ser 
65                  70                  75                  80  


Glu Gly Leu Arg Ala Pro Gly Arg Thr Arg Leu Leu Gly Ile Thr Pro 
                85                  90                  95      


Ile Leu Gly Arg Gln Gly Phe Phe Val Gly Asp Arg Ser Pro Ile Thr 
            100                 105                 110         


Val Ala Ala Asp Leu Ala Gly Arg Arg Ile Gly Val Ser Ala Ser Ala 
        115                 120                 125             


Ile Arg Ile Leu Arg Gly Glu Leu Gly Asp Tyr Leu Gln Leu Asp Pro 
    130                 135                 140                 


Trp Arg Gln Thr Leu Val Ala Leu Gly Ser Trp Glu Ala Arg Ala Leu 
145                 150                 155                 160 


Leu His Thr Leu Glu His Gly Glu Leu Asp Val Asp Asp Val Glu Leu 
                165                 170                 175     


Val Pro Ile Asn Ser Pro Gly Val Asp Val Pro Ala Glu Gln Leu Glu 
            180                 185                 190         


Asp Ala Ala Thr Leu Lys Gly Ala Asp Leu Phe Pro Asp Val Ala Ala 
        195                 200                 205             


Gly Gln Ala Ala Val Leu Asp Arg Gly Glu Val Asp Ala Leu Phe Ser 
    210                 215                 220                 


Trp Leu Pro Trp Ala Ala Glu Leu Glu Gly Thr Gly Ala Arg Pro Val 
225                 230                 235                 240 


Val Asp Leu Gly Leu Asp Glu Arg Asn Ala Tyr Ala Ser Val Trp Thr 
                245                 250                 255     


Val Ser Ser Glu Leu Val Val Asp Arg Pro Asp Leu Val Gln Arg Leu 
            260                 265                 270         


Val Asp Ala Val Val Asp Ala Gly Leu Trp Ala Arg Asp His Gly Asp 
        275                 280                 285             


Ala Val Thr Arg Leu His Ala Ala Asn Leu Gly Val Ser Pro Asp Ala 
    290                 295                 300                 


Val Gly His Gly Phe Gly Ala Asp Phe Gln Gln Arg Leu Val Pro Arg 
305                 310                 315                 320 


Leu Asp Pro Asp Ala Val Ala Leu Leu Asp Arg Thr Gln Gln Phe Leu 
                325                 330                 335     


Leu Ser Asn Gln Leu Leu Gln Glu Pro Val Ala Leu Asp Gln Trp Ala 
            340                 345                 350         


Ala Pro Glu Phe Leu Asn Thr Ser Leu Asn Arg His Arg 
        355                 360                 365 


<210>  11
<211>  1341
<212>  DNA
<213>  Artificial sequence

<220>
<223>  BclB+ DszC fusion DNA

<400>  11
ttgaaacaga atgacaaatt atggttggat aaaggcataa taggcccaga aaatattgga       60

cctaccttcc cggttcttcc cccagagctc atgactctgt ccgttgaaaa gcagcacgtt      120

cgacccggcg atgccgacaa cgatcccgtc gcagtcgccc gtgggctcgc cgagaagtgg      180

cgagccaccg ccgtcgagcg tgatcgcgcc gggggttcgg caacagtcga gcgcgaagac      240

ctgcgcgcga gcggcctgct gtcgctgctg atcccacgcc agtacggcgg gtggggcgcg      300

gactggccca ccgccatcga agtggtccga gaaatcgccg cagccgatgg atctttggga      360

cacctgctcg gctaccacct cagcagcgcc ccgatgatcg aactgttcgg ctcgcaggaa      420

caagaacaac ggctataccg gcaaatcgcg cagaacgact ggtggaccgg gaacgcctcc      480

agcgaaaaca acagccacgt gctggactgg aaggtcagcg ccagcccgac cgaggacggc      540

ggttatctgc tcaacggcac caagcacttc tgcagcggcg ccaagggttc ggacctgttg      600

ctggtcttcg gcgtcatcca ggacgattct ccgcagcagg gcgcgatcat cgcggccgtc      660

atcccgacat cccggcacgg cgttcaggtc aacgacgact gggccgccat cggcatgcgg      720

cagaccgaca gcggatccac cgatttccac agcgtcaagg tcgagcctga cgaagtgctg      780

ggtgagccca acgccttcat tgtcgccttc atccagtccg agcgcggcag cctcttcgcg      840

cccatagtgc aattgatctt cgccaacgtc tatttaggga tcgcgcacgg cgcactcgac      900

gccgcccggg agtacacccg cactcaggcc aggccatgga caccggccgg ggttcaacag      960

gcaaccgagg atccctacgt cctgcgcgcc tatggtgaat tcaccatcgc attgcagggc     1020

gccgacgccg ctgcccgcga agcggcccat ctgctgcaga cggtgtggga caagggcgat     1080

gcgctcaccc ccgaggaccg cggcgaactg atggtgaaga tctccggggt caaagcgttg     1140

gccacgaacg ccgccctcga cgtcaacagc ggaatcttcg aggtgatcgg agcgcgcgga     1200

acacatccca agtacggttt cgaccgcttc tggcgcaacg tacgcacgca caccctccac     1260

gatccggtgt cctacaagat cgccgacgtc ggcaaacaca ccctcaacgg gcagtacccg     1320

attcccggct tcacctccta g                                               1341


<210>  12
<211>  446
<212>  PRT
<213>  artificial sequence

<220>
<223>  BclB+ DszC fusion polypeptide

<400>  12

Leu Lys Gln Asn Asp Lys Leu Trp Leu Asp Lys Gly Ile Ile Gly Pro 
1               5                   10                  15      


Glu Asn Ile Gly Pro Thr Phe Pro Val Leu Pro Pro Glu Leu Met Thr 
            20                  25                  30          


Leu Ser Val Glu Lys Gln His Val Arg Pro Gly Asp Ala Asp Asn Asp 
        35                  40                  45              


Pro Val Ala Val Ala Arg Gly Leu Ala Glu Lys Trp Arg Ala Thr Ala 
    50                  55                  60                  


Val Glu Arg Asp Arg Ala Gly Gly Ser Ala Thr Val Glu Arg Glu Asp 
65                  70                  75                  80  


Leu Arg Ala Ser Gly Leu Leu Ser Leu Leu Ile Pro Arg Gln Tyr Gly 
                85                  90                  95      


Gly Trp Gly Ala Asp Trp Pro Thr Ala Ile Glu Val Val Arg Glu Ile 
            100                 105                 110         


Ala Ala Ala Asp Gly Ser Leu Gly His Leu Leu Gly Tyr His Leu Ser 
        115                 120                 125             


Ser Ala Pro Met Ile Glu Leu Phe Gly Ser Gln Glu Gln Glu Gln Arg 
    130                 135                 140                 


Leu Tyr Arg Gln Ile Ala Gln Asn Asp Trp Trp Thr Gly Asn Ala Ser 
145                 150                 155                 160 


Ser Glu Asn Asn Ser His Val Leu Asp Trp Lys Val Ser Ala Ser Pro 
                165                 170                 175     


Thr Glu Asp Gly Gly Tyr Leu Leu Asn Gly Thr Lys His Phe Cys Ser 
            180                 185                 190         


Gly Ala Lys Gly Ser Asp Leu Leu Leu Val Phe Gly Val Ile Gln Asp 
        195                 200                 205             


Asp Ser Pro Gln Gln Gly Ala Ile Ile Ala Ala Val Ile Pro Thr Ser 
    210                 215                 220                 


Arg His Gly Val Gln Val Asn Asp Asp Trp Ala Ala Ile Gly Met Arg 
225                 230                 235                 240 


Gln Thr Asp Ser Gly Ser Thr Asp Phe His Ser Val Lys Val Glu Pro 
                245                 250                 255     


Asp Glu Val Leu Gly Glu Pro Asn Ala Phe Ile Val Ala Phe Ile Gln 
            260                 265                 270         


Ser Glu Arg Gly Ser Leu Phe Ala Pro Ile Val Gln Leu Ile Phe Ala 
        275                 280                 285             


Asn Val Tyr Leu Gly Ile Ala His Gly Ala Leu Asp Ala Ala Arg Glu 
    290                 295                 300                 


Tyr Thr Arg Thr Gln Ala Arg Pro Trp Thr Pro Ala Gly Val Gln Gln 
305                 310                 315                 320 


Ala Thr Glu Asp Pro Tyr Val Leu Arg Ala Tyr Gly Glu Phe Thr Ile 
                325                 330                 335     


Ala Leu Gln Gly Ala Asp Ala Ala Ala Arg Glu Ala Ala His Leu Leu 
            340                 345                 350         


Gln Thr Val Trp Asp Lys Gly Asp Ala Leu Thr Pro Glu Asp Arg Gly 
        355                 360                 365             


Glu Leu Met Val Lys Ile Ser Gly Val Lys Ala Leu Ala Thr Asn Ala 
    370                 375                 380                 


Ala Leu Asp Val Asn Ser Gly Ile Phe Glu Val Ile Gly Ala Arg Gly 
385                 390                 395                 400 


Thr His Pro Lys Tyr Gly Phe Asp Arg Phe Trp Arg Asn Val Arg Thr 
                405                 410                 415     


His Thr Leu His Asp Pro Val Ser Tyr Lys Ile Ala Asp Val Gly Lys 
            420                 425                 430         


His Thr Leu Asn Gly Gln Tyr Pro Ile Pro Gly Phe Thr Ser 
        435                 440                 445     


<210>  13
<211>  669
<212>  DNA
<213>  Artificial sequence

<220>
<223>  BclB+ DszD fusion DNA

<400>  13
ttgaaacaga atgacaaatt atggttggat aaaggcataa taggcccaga aaatattgga       60

cctaccttcc cggttcttcc cccagagctc atgtctgaca agccgaatgc cgtttccagc      120

cacaccaccc ccgacgtccc cgaagtagca gcgacgcccg agttgtccac cggcatctgc      180

gccggtgact accgtgcagc gcttcgccgc caccccgcag gtgtcaccgt cgtgaccctc      240

gattcgggta ccggcccggt gggtttcacc gccacctcgt tctcgtccgt ctccctggag      300

ccgccgctcg tctcgttcaa catcgcggag acgtcgtcga gcatcaatgc gctcaaggca      360

gccgagtcct tggtgatcca ccttctcggc gaacatcagc aacatctggc ccagcgcttt      420

gcgcgtagcg ccgatcagcg ttttgcagac gagtcactgt gggcagtgct cgacaccggg      480

gagccggttc tgcacggcac ccccagctgg atgcgcgtca aggtcgacca gttgatccct      540

gtcggcgacc acacgctggt catcggactc gtcacgcggg ttcacgccga agaggacgac      600

gaatccgctg ccgcaccgct gctctaccac gagggcaagt actaccgccc gactccgtta      660

ggtcaatag                                                              669


<210>  14
<211>  222
<212>  PRT
<213>  Artificial sequence

<220>
<223>  BclB+ DszD fusion polypeptide

<400>  14

Leu Lys Gln Asn Asp Lys Leu Trp Leu Asp Lys Gly Ile Ile Gly Pro 
1               5                   10                  15      


Glu Asn Ile Gly Pro Thr Phe Pro Val Leu Pro Pro Glu Leu Met Ser 
            20                  25                  30          


Asp Lys Pro Asn Ala Val Ser Ser His Thr Thr Pro Asp Val Pro Glu 
        35                  40                  45              


Val Ala Ala Thr Pro Glu Leu Ser Thr Gly Ile Cys Ala Gly Asp Tyr 
    50                  55                  60                  


Arg Ala Ala Leu Arg Arg His Pro Ala Gly Val Thr Val Val Thr Leu 
65                  70                  75                  80  


Asp Ser Gly Thr Gly Pro Val Gly Phe Thr Ala Thr Ser Phe Ser Ser 
                85                  90                  95      


Val Ser Leu Glu Pro Pro Leu Val Ser Phe Asn Ile Ala Glu Thr Ser 
            100                 105                 110         


Ser Ser Ile Asn Ala Leu Lys Ala Ala Glu Ser Leu Val Ile His Leu 
        115                 120                 125             


Leu Gly Glu His Gln Gln His Leu Ala Gln Arg Phe Ala Arg Ser Ala 
    130                 135                 140                 


Asp Gln Arg Phe Ala Asp Glu Ser Leu Trp Ala Val Leu Asp Thr Gly 
145                 150                 155                 160 


Glu Pro Val Leu His Gly Thr Pro Ser Trp Met Arg Val Lys Val Asp 
                165                 170                 175     


Gln Leu Ile Pro Val Gly Asp His Thr Leu Val Ile Gly Leu Val Thr 
            180                 185                 190         


Arg Val His Ala Glu Glu Asp Asp Glu Ser Ala Ala Ala Pro Leu Leu 
        195                 200                 205             


Tyr His Glu Gly Lys Tyr Tyr Arg Pro Thr Pro Leu Gly Gln 
    210                 215                 220         


<210>  15
<211>  1515
<212>  DNA
<213>  Artificial sequence

<220>
<223>  BclB+ DszA fusion DNA

<400>  15
ttgaaacaga atgacaaatt atggttggat aaaggcataa taggcccaga aaatattgga       60

cctaccttcc cggttcttcc cccagagctc atggctcaac ggcgacaact gcatctggcc      120

ggtttcttct cggctggcaa tgtgactcat gcacatgggg cgtggcggca tacggacgcg      180

tcgaatggtt ttttgaccgg gaagtactac caacacatcg cccgcactct ggagcgcggc      240

aagttcgatc tgttgttcct gcctgacgga ttggccgtcg aggacagcta cggcgacgac      300

ctgcgaaccg gtgtcggcct cggcgggcag ggtgcagtcg ccttggagcc ggccagcgtg      360

atagcgacca tggccgcggt caccgaacac ctcggtctcg gggcaaccat ttcggcgacc      420

tactatcccc cgtatcacgt tgctcgggtg ttcgcgacgc tcgatcaatt gtcggggggc      480

cgggtttcgt ggaacgtggt cacctcgctc aacgacgctg aagcgcgcaa cttcggtatt      540

gatcagcatc tggaacacga cgcccgttac gaccgcgccg atgagttctt ggatgcggtc      600

aagaaactct ggaacagctg ggatgaggat gcgctcgtac tggacaaggc ggcaggcgtg      660

ttcgccgatc ccacgaaggt tcactacgtg gatcaccacg gggagtggct gaatgtgcgc      720

gggcctctgc aggtgcctcg ttcacctcag ggtgagccgg tgatcctgca ggccgggttg      780

tcgccgcgag gtcggcgctt cgccgggcgg tgggccgagg ccgttttcag tgtcgcaccc      840

gatctgggat tgatgcaggc tacatatcac gacatcaaag cccaggtgaa ggctgcgggg      900

cgtgatccgg atcagacgaa gatcttcacc gccgtgatgc cggtgttggg cgagaccgag      960

gccgtggcgc aggaccggct ggaatacctg aacagtctgg tgcatccgga ggtcggactt     1020

tcgacgctgt ctagtcatac cggcatcaac ttggcggagt acccgttgga cacgccgatc     1080

acgacgatcc tgcgggatct gcaggatcgc aacgtgccga cgcaactgca catgttcgcg     1140

gccgcgatgc acgccgagga gctcactttg gcggagctgg gtcggcggta tggaaccaac     1200

gtgggcttcg ttccgcagtg ggccggcacg gccgagcaga tcgccgagga gttgatccgc     1260

cacttcgacg ccggcgccgc ggatggcttc attgtttctc cggcctttct gcctggcgcg     1320

tatgacgaat tcgtcgacca ggtggtgccg gttctgcagg accgcggcta cttccgcacc     1380

gaatacgagg gcaacaccct gcgcgaccac ctgggtctgc gcgaaccacg accgttagga     1440

caaccttcat ggcaggccgc ctcagccccg gaaaccccgg ttcagaactt gataccggca     1500

tcctcgacac actga                                                      1515


<210>  16
<211>  504
<212>  PRT
<213>  Artificial sequence

<220>
<223>  BclB+ DszA fusion polypeptide

<400>  16

Leu Lys Gln Asn Asp Lys Leu Trp Leu Asp Lys Gly Ile Ile Gly Pro 
1               5                   10                  15      


Glu Asn Ile Gly Pro Thr Phe Pro Val Leu Pro Pro Glu Leu Met Ala 
            20                  25                  30          


Gln Arg Arg Gln Leu His Leu Ala Gly Phe Phe Ser Ala Gly Asn Val 
        35                  40                  45              


Thr His Ala His Gly Ala Trp Arg His Thr Asp Ala Ser Asn Gly Phe 
    50                  55                  60                  


Leu Thr Gly Lys Tyr Tyr Gln His Ile Ala Arg Thr Leu Glu Arg Gly 
65                  70                  75                  80  


Lys Phe Asp Leu Leu Phe Leu Pro Asp Gly Leu Ala Val Glu Asp Ser 
                85                  90                  95      


Tyr Gly Asp Asp Leu Arg Thr Gly Val Gly Leu Gly Gly Gln Gly Ala 
            100                 105                 110         


Val Ala Leu Glu Pro Ala Ser Val Ile Ala Thr Met Ala Ala Val Thr 
        115                 120                 125             


Glu His Leu Gly Leu Gly Ala Thr Ile Ser Ala Thr Tyr Tyr Pro Pro 
    130                 135                 140                 


Tyr His Val Ala Arg Val Phe Ala Thr Leu Asp Gln Leu Ser Gly Gly 
145                 150                 155                 160 


Arg Val Ser Trp Asn Val Val Thr Ser Leu Asn Asp Ala Glu Ala Arg 
                165                 170                 175     


Asn Phe Gly Ile Asp Gln His Leu Glu His Asp Ala Arg Tyr Asp Arg 
            180                 185                 190         


Ala Asp Glu Phe Leu Asp Ala Val Lys Lys Leu Trp Asn Ser Trp Asp 
        195                 200                 205             


Glu Asp Ala Leu Val Leu Asp Lys Ala Ala Gly Val Phe Ala Asp Pro 
    210                 215                 220                 


Thr Lys Val His Tyr Val Asp His His Gly Glu Trp Leu Asn Val Arg 
225                 230                 235                 240 


Gly Pro Leu Gln Val Pro Arg Ser Pro Gln Gly Glu Pro Val Ile Leu 
                245                 250                 255     


Gln Ala Gly Leu Ser Pro Arg Gly Arg Arg Phe Ala Gly Arg Trp Ala 
            260                 265                 270         


Glu Ala Val Phe Ser Val Ala Pro Asp Leu Gly Leu Met Gln Ala Thr 
        275                 280                 285             


Tyr His Asp Ile Lys Ala Gln Val Lys Ala Ala Gly Arg Asp Pro Asp 
    290                 295                 300                 


Gln Thr Lys Ile Phe Thr Ala Val Met Pro Val Leu Gly Glu Thr Glu 
305                 310                 315                 320 


Ala Val Ala Gln Asp Arg Leu Glu Tyr Leu Asn Ser Leu Val His Pro 
                325                 330                 335     


Glu Val Gly Leu Ser Thr Leu Ser Ser His Thr Gly Ile Asn Leu Ala 
            340                 345                 350         


Glu Tyr Pro Leu Asp Thr Pro Ile Thr Thr Ile Leu Arg Asp Leu Gln 
        355                 360                 365             


Asp Arg Asn Val Pro Thr Gln Leu His Met Phe Ala Ala Ala Met His 
    370                 375                 380                 


Ala Glu Glu Leu Thr Leu Ala Glu Leu Gly Arg Arg Tyr Gly Thr Asn 
385                 390                 395                 400 


Val Gly Phe Val Pro Gln Trp Ala Gly Thr Ala Glu Gln Ile Ala Glu 
                405                 410                 415     


Glu Leu Ile Arg His Phe Asp Ala Gly Ala Ala Asp Gly Phe Ile Val 
            420                 425                 430         


Ser Pro Ala Phe Leu Pro Gly Ala Tyr Asp Glu Phe Val Asp Gln Val 
        435                 440                 445             


Val Pro Val Leu Gln Asp Arg Gly Tyr Phe Arg Thr Glu Tyr Glu Gly 
    450                 455                 460                 


Asn Thr Leu Arg Asp His Leu Gly Leu Arg Glu Pro Arg Pro Leu Gly 
465                 470                 475                 480 


Gln Pro Ser Trp Gln Ala Ala Ser Ala Pro Glu Thr Pro Val Gln Asn 
                485                 490                 495     


Leu Ile Pro Ala Ser Ser Thr His 
            500                 


<210>  17
<211>  1188
<212>  DNA
<213>  Artificial sequence

<220>
<223>  BclB+ DszB fusion DNA

<400>  17
ttgaaacaga atgacaaatt atggttggat aaaggcataa taggcccaga aaatattgga       60

cctaccttcc cggttcttcc cccagagctc atggcaggcc gcctcagccc cggaaacccc      120

ggttcagaac ttgataccgg catcctcgac acactgacct acagcaactg cccgataccc      180

aacgcgctgc tcacggcgtg ggaatcaggt ttcctcgacg ccgccggcat cgaactcgac      240

atcctcagcg gcaagcaggg aacggtccac ttcacctacg accagcccgc ctacacccgc      300

tatggcggtg agatcccgcc actgctcagc gaggggttgc gggcacccgg acgcacgcgt      360

ctactcggca tcaccccgat cctggggcgt cagggcttct tcgtcggcga tcgcagcccg      420

atcacagtgg ccgccgacct tgccggacgc cgaataggag tgtcggcttc ggcgattcgc      480

atcctgcgtg gcgaactggg ggactatctc cagctggatc cctggcggca gacgctggta      540

gcgctgggct cgtgggaggc gcgcgcgttg ttgcacaccc tcgagcacgg tgagctcgat      600

gtggacgacg tcgagctggt gccgatcaac agtcctggcg tcgatgtccc cgccgagcag      660

ttggaagacg ccgcgaccct caagggtgcg gacctgtttc ccgatgtggc cgcaggccag      720

gccgcggtgc tggaccgcgg tgaggttgac gccctgttca gttggttgcc ctgggctgcg      780

gagttggaag gcaccggcgc ccgcccggtg gtggatctcg gcctcgatga gcgcaatgcc      840

tatgccagcg tgtggacggt cagcagcgaa ctggtcgttg accggcctga cctcgttcaa      900

cgactggtcg acgcagtcgt cgacgccggg ttgtgggcac gcgaccacgg cgatgcggtg      960

acccgcctgc acgccgcgaa cctgggcgta tcccccgacg cagtcggcca cggattcggc     1020

gccgacttcc agcagcgttt ggttccgcgc ctggatcccg acgcggtcgc cctgctggat     1080

cgcacacagc aattcctgct cagcaaccag ttgctgcagg agcccgtcgc cctcgatcag     1140

tgggcggctc cggaatttct gaacaccagc ctcaaccgtc accgatag                  1188


<210>  18
<211>  395
<212>  PRT
<213>  Artificial sequence

<220>
<223>  BclB+ DszB fusion polypeptide

<400>  18

Leu Lys Gln Asn Asp Lys Leu Trp Leu Asp Lys Gly Ile Ile Gly Pro 
1               5                   10                  15      


Glu Asn Ile Gly Pro Thr Phe Pro Val Leu Pro Pro Glu Leu Met Ala 
            20                  25                  30          


Gly Arg Leu Ser Pro Gly Asn Pro Gly Ser Glu Leu Asp Thr Gly Ile 
        35                  40                  45              


Leu Asp Thr Leu Thr Tyr Ser Asn Cys Pro Ile Pro Asn Ala Leu Leu 
    50                  55                  60                  


Thr Ala Trp Glu Ser Gly Phe Leu Asp Ala Ala Gly Ile Glu Leu Asp 
65                  70                  75                  80  


Ile Leu Ser Gly Lys Gln Gly Thr Val His Phe Thr Tyr Asp Gln Pro 
                85                  90                  95      


Ala Tyr Thr Arg Tyr Gly Gly Glu Ile Pro Pro Leu Leu Ser Glu Gly 
            100                 105                 110         


Leu Arg Ala Pro Gly Arg Thr Arg Leu Leu Gly Ile Thr Pro Ile Leu 
        115                 120                 125             


Gly Arg Gln Gly Phe Phe Val Gly Asp Arg Ser Pro Ile Thr Val Ala 
    130                 135                 140                 


Ala Asp Leu Ala Gly Arg Arg Ile Gly Val Ser Ala Ser Ala Ile Arg 
145                 150                 155                 160 


Ile Leu Arg Gly Glu Leu Gly Asp Tyr Leu Gln Leu Asp Pro Trp Arg 
                165                 170                 175     


Gln Thr Leu Val Ala Leu Gly Ser Trp Glu Ala Arg Ala Leu Leu His 
            180                 185                 190         


Thr Leu Glu His Gly Glu Leu Asp Val Asp Asp Val Glu Leu Val Pro 
        195                 200                 205             


Ile Asn Ser Pro Gly Val Asp Val Pro Ala Glu Gln Leu Glu Asp Ala 
    210                 215                 220                 


Ala Thr Leu Lys Gly Ala Asp Leu Phe Pro Asp Val Ala Ala Gly Gln 
225                 230                 235                 240 


Ala Ala Val Leu Asp Arg Gly Glu Val Asp Ala Leu Phe Ser Trp Leu 
                245                 250                 255     


Pro Trp Ala Ala Glu Leu Glu Gly Thr Gly Ala Arg Pro Val Val Asp 
            260                 265                 270         


Leu Gly Leu Asp Glu Arg Asn Ala Tyr Ala Ser Val Trp Thr Val Ser 
        275                 280                 285             


Ser Glu Leu Val Val Asp Arg Pro Asp Leu Val Gln Arg Leu Val Asp 
    290                 295                 300                 


Ala Val Val Asp Ala Gly Leu Trp Ala Arg Asp His Gly Asp Ala Val 
305                 310                 315                 320 


Thr Arg Leu His Ala Ala Asn Leu Gly Val Ser Pro Asp Ala Val Gly 
                325                 330                 335     


His Gly Phe Gly Ala Asp Phe Gln Gln Arg Leu Val Pro Arg Leu Asp 
            340                 345                 350         


Pro Asp Ala Val Ala Leu Leu Asp Arg Thr Gln Gln Phe Leu Leu Ser 
        355                 360                 365             


Asn Gln Leu Leu Gln Glu Pro Val Ala Leu Asp Gln Trp Ala Ala Pro 
    370                 375                 380                 


Glu Phe Leu Asn Thr Ser Leu Asn Arg His Arg 
385                 390                 395 


<210>  19
<211>  101
<212>  DNA
<213>  Artificial sequence

<220>
<223>  N-terminal region of BclA gene

<400>  19
atgtcaaata ataattattc aaatggatta aaccccgatg aatctttata gctagtgcat       60

ttgaccctaa tcttgtagga cctacattac caccgatacc a                          101


<210>  20
<211>  36
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal domain of BclA protein

<400>  20

Met Ser Asn Asn Asn Tyr Ser Asn Gly Leu Asn Pro Asp Glu Ser Leu 
1               5                   10                  15      


Ser Ala Ser Ala Phe Asp Pro Asn Leu Val Gly Pro Thr Leu Pro Pro 
            20                  25                  30          


Ile Pro Pro Phe 
        35      


<210>  21
<211>  1359
<212>  DNA
<213>  Artificial sequence

<220>
<223>  BclA+ DszC fusion DNA

<400>  21
atgtcaaata ataattattc aaatggatta aaccccgatg aatctttatc agctagtgca       60

tttgacccta atcttgtagg acctacatta ccaccgatac cagagctcat gactctgtcc      120

gttgaaaagc agcacgttcg acccggcgat gccgacaacg atcccgtcgc agtcgcccgt      180

gggctcgccg agaagtggcg agccaccgcc gtcgagcgtg atcgcgccgg gggttcggca      240

acagtcgagc gcgaagacct gcgcgcgagc ggcctgctgt cgctgctgat cccacgccag      300

tacggcgggt ggggcgcgga ctggcccacc gccatcgaag tggtccgaga aatcgccgca      360

gccgatggat ctttgggaca cctgctcggc taccacctca gcagcgcccc gatgatcgaa      420

ctgttcggct cgcaggaaca agaacaacgg ctataccggc aaatcgcgca gaacgactgg      480

tggaccggga acgcctccag cgaaaacaac agccacgtgc tggactggaa ggtcagcgcc      540

agcccgaccg aggacggcgg ttatctgctc aacggcacca agcacttctg cagcggcgcc      600

aagggttcgg acctgttgct ggtcttcggc gtcatccagg acgattctcc gcagcagggc      660

gcgatcatcg cggccgtcat cccgacatcc cggcacggcg ttcaggtcaa cgacgactgg      720

gccgccatcg gcatgcggca gaccgacagc ggatccaccg atttccacag cgtcaaggtc      780

gagcctgacg aagtgctggg tgagcccaac gccttcattg tcgccttcat ccagtccgag      840

cgcggcagcc tcttcgcgcc catagtgcaa ttgatcttcg ccaacgtcta tttagggatc      900

gcgcacggcg cactcgacgc cgcccgggag tacacccgca ctcaggccag gccatggaca      960

ccggccgggg ttcaacaggc aaccgaggat ccctacgtcc tgcgcgccta tggtgaattc     1020

accatcgcat tgcagggcgc cgacgccgct gcccgcgaag cggcccatct gctgcagacg     1080

gtgtgggaca agggcgatgc gctcaccccc gaggaccgcg gcgaactgat ggtgaagatc     1140

tccggggtca aagcgttggc cacgaacgcc gccctcgacg tcaacagcgg aatcttcgag     1200

gtgatcggag cgcgcggaac acatcccaag tacggtttcg accgcttctg gcgcaacgta     1260

cgcacgcaca ccctccacga tccggtgtcc tacaagatcg ccgacgtcgg caaacacacc     1320

ctcaacgggc agtacccgat tcccggcttc acctcctag                            1359


<210>  22
<211>  452
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BclA+ DszC fusion polypeptide

<400>  22

Met Ser Asn Asn Asn Tyr Ser Asn Gly Leu Asn Pro Asp Glu Ser Leu 
1               5                   10                  15      


Ser Ala Ser Ala Phe Asp Pro Asn Leu Val Gly Pro Thr Leu Pro Pro 
            20                  25                  30          


Ile Pro Glu Leu Met Thr Leu Ser Val Glu Lys Gln His Val Arg Pro 
        35                  40                  45              


Gly Asp Ala Asp Asn Asp Pro Val Ala Val Ala Arg Gly Leu Ala Glu 
    50                  55                  60                  


Lys Trp Arg Ala Thr Ala Val Glu Arg Asp Arg Ala Gly Gly Ser Ala 
65                  70                  75                  80  


Thr Val Glu Arg Glu Asp Leu Arg Ala Ser Gly Leu Leu Ser Leu Leu 
                85                  90                  95      


Ile Pro Arg Gln Tyr Gly Gly Trp Gly Ala Asp Trp Pro Thr Ala Ile 
            100                 105                 110         


Glu Val Val Arg Glu Ile Ala Ala Ala Asp Gly Ser Leu Gly His Leu 
        115                 120                 125             


Leu Gly Tyr His Leu Ser Ser Ala Pro Met Ile Glu Leu Phe Gly Ser 
    130                 135                 140                 


Gln Glu Gln Glu Gln Arg Leu Tyr Arg Gln Ile Ala Gln Asn Asp Trp 
145                 150                 155                 160 


Trp Thr Gly Asn Ala Ser Ser Glu Asn Asn Ser His Val Leu Asp Trp 
                165                 170                 175     


Lys Val Ser Ala Ser Pro Thr Glu Asp Gly Gly Tyr Leu Leu Asn Gly 
            180                 185                 190         


Thr Lys His Phe Cys Ser Gly Ala Lys Gly Ser Asp Leu Leu Leu Val 
        195                 200                 205             


Phe Gly Val Ile Gln Asp Asp Ser Pro Gln Gln Gly Ala Ile Ile Ala 
    210                 215                 220                 


Ala Val Ile Pro Thr Ser Arg His Gly Val Gln Val Asn Asp Asp Trp 
225                 230                 235                 240 


Ala Ala Ile Gly Met Arg Gln Thr Asp Ser Gly Ser Thr Asp Phe His 
                245                 250                 255     


Ser Val Lys Val Glu Pro Asp Glu Val Leu Gly Glu Pro Asn Ala Phe 
            260                 265                 270         


Ile Val Ala Phe Ile Gln Ser Glu Arg Gly Ser Leu Phe Ala Pro Ile 
        275                 280                 285             


Val Gln Leu Ile Phe Ala Asn Val Tyr Leu Gly Ile Ala His Gly Ala 
    290                 295                 300                 


Leu Asp Ala Ala Arg Glu Tyr Thr Arg Thr Gln Ala Arg Pro Trp Thr 
305                 310                 315                 320 


Pro Ala Gly Val Gln Gln Ala Thr Glu Asp Pro Tyr Val Leu Arg Ala 
                325                 330                 335     


Tyr Gly Glu Phe Thr Ile Ala Leu Gln Gly Ala Asp Ala Ala Ala Arg 
            340                 345                 350         


Glu Ala Ala His Leu Leu Gln Thr Val Trp Asp Lys Gly Asp Ala Leu 
        355                 360                 365             


Thr Pro Glu Asp Arg Gly Glu Leu Met Val Lys Ile Ser Gly Val Lys 
    370                 375                 380                 


Ala Leu Ala Thr Asn Ala Ala Leu Asp Val Asn Ser Gly Ile Phe Glu 
385                 390                 395                 400 


Val Ile Gly Ala Arg Gly Thr His Pro Lys Tyr Gly Phe Asp Arg Phe 
                405                 410                 415     


Trp Arg Asn Val Arg Thr His Thr Leu His Asp Pro Val Ser Tyr Lys 
            420                 425                 430         


Ile Ala Asp Val Gly Lys His Thr Leu Asn Gly Gln Tyr Pro Ile Pro 
        435                 440                 445             


Gly Phe Thr Ser 
    450         


<210>  23
<211>  687
<212>  DNA
<213>  Artificial sequence

<220>
<223>  BclA+ DszD fusion DNA

<400>  23
atgtcaaata ataattattc aaatggatta aaccccgatg aatctttatc agctagtgca       60

tttgacccta atcttgtagg acctacatta ccaccgatac cagagctcat gtctgacaag      120

ccgaatgccg tttccagcca caccaccccc gacgtccccg aagtagcagc gacgcccgag      180

ttgtccaccg gcatctgcgc cggtgactac cgtgcagcgc ttcgccgcca ccccgcaggt      240

gtcaccgtcg tgaccctcga ttcgggtacc ggcccggtgg gtttcaccgc cacctcgttc      300

tcgtccgtct ccctggagcc gccgctcgtc tcgttcaaca tcgcggagac gtcgtcgagc      360

atcaatgcgc tcaaggcagc cgagtccttg gtgatccacc ttctcggcga acatcagcaa      420

catctggccc agcgctttgc gcgtagcgcc gatcagcgtt ttgcagacga gtcactgtgg      480

gcagtgctcg acaccgggga gccggttctg cacggcaccc ccagctggat gcgcgtcaag      540

gtcgaccagt tgatccctgt cggcgaccac acgctggtca tcggactcgt cacgcgggtt      600

cacgccgaag aggacgacga atccgctgcc gcaccgctgc tctaccacga gggcaagtac      660

taccgcccga ctccgttagg tcaatag                                          687


<210>  24
<211>  228
<212>  PRT
<213>  Artificial sequence

<220>
<223>  BclA+ DszD fusion polypeptide

<400>  24

Met Ser Asn Asn Asn Tyr Ser Asn Gly Leu Asn Pro Asp Glu Ser Leu 
1               5                   10                  15      


Ser Ala Ser Ala Phe Asp Pro Asn Leu Val Gly Pro Thr Leu Pro Pro 
            20                  25                  30          


Ile Pro Glu Leu Met Ser Asp Lys Pro Asn Ala Val Ser Ser His Thr 
        35                  40                  45              


Thr Pro Asp Val Pro Glu Val Ala Ala Thr Pro Glu Leu Ser Thr Gly 
    50                  55                  60                  


Ile Cys Ala Gly Asp Tyr Arg Ala Ala Leu Arg Arg His Pro Ala Gly 
65                  70                  75                  80  


Val Thr Val Val Thr Leu Asp Ser Gly Thr Gly Pro Val Gly Phe Thr 
                85                  90                  95      


Ala Thr Ser Phe Ser Ser Val Ser Leu Glu Pro Pro Leu Val Ser Phe 
            100                 105                 110         


Asn Ile Ala Glu Thr Ser Ser Ser Ile Asn Ala Leu Lys Ala Ala Glu 
        115                 120                 125             


Ser Leu Val Ile His Leu Leu Gly Glu His Gln Gln His Leu Ala Gln 
    130                 135                 140                 


Arg Phe Ala Arg Ser Ala Asp Gln Arg Phe Ala Asp Glu Ser Leu Trp 
145                 150                 155                 160 


Ala Val Leu Asp Thr Gly Glu Pro Val Leu His Gly Thr Pro Ser Trp 
                165                 170                 175     


Met Arg Val Lys Val Asp Gln Leu Ile Pro Val Gly Asp His Thr Leu 
            180                 185                 190         


Val Ile Gly Leu Val Thr Arg Val His Ala Glu Glu Asp Asp Glu Ser 
        195                 200                 205             


Ala Ala Ala Pro Leu Leu Tyr His Glu Gly Lys Tyr Tyr Arg Pro Thr 
    210                 215                 220                 


Pro Leu Gly Gln 
225             


<210>  25
<211>  1533
<212>  DNA
<213>  Artificial sequence

<220>
<223>  BclA+ DszA fusion DNA

<400>  25
atgtcaaata ataattattc aaatggatta aaccccgatg aatctttatc agctagtgca       60

tttgacccta atcttgtagg acctacatta ccaccgatac cagagctcat ggctcaacgg      120

cgacaactgc atctggccgg tttcttctcg gctggcaatg tgactcatgc acatggggcg      180

tggcggcata cggacgcgtc gaatggtttt ttgaccggga agtactacca acacatcgcc      240

cgcactctgg agcgcggcaa gttcgatctg ttgttcctgc ctgacggatt ggccgtcgag      300

gacagctacg gcgacgacct gcgaaccggt gtcggcctcg gcgggcaggg tgcagtcgcc      360

ttggagccgg ccagcgtgat agcgaccatg gccgcggtca ccgaacacct cggtctcggg      420

gcaaccattt cggcgaccta ctatcccccg tatcacgttg ctcgggtgtt cgcgacgctc      480

gatcaattgt cggggggccg ggtttcgtgg aacgtggtca cctcgctcaa cgacgctgaa      540

gcgcgcaact tcggtattga tcagcatctg gaacacgacg cccgttacga ccgcgccgat      600

gagttcttgg atgcggtcaa gaaactctgg aacagctggg atgaggatgc gctcgtactg      660

gacaaggcgg caggcgtgtt cgccgatccc acgaaggttc actacgtgga tcaccacggg      720

gagtggctga atgtgcgcgg gcctctgcag gtgcctcgtt cacctcaggg tgagccggtg      780

atcctgcagg ccgggttgtc gccgcgaggt cggcgcttcg ccgggcggtg ggccgaggcc      840

gttttcagtg tcgcacccga tctgggattg atgcaggcta catatcacga catcaaagcc      900

caggtgaagg ctgcggggcg tgatccggat cagacgaaga tcttcaccgc cgtgatgccg      960

gtgttgggcg agaccgaggc cgtggcgcag gaccggctgg aatacctgaa cagtctggtg     1020

catccggagg tcggactttc gacgctgtct agtcataccg gcatcaactt ggcggagtac     1080

ccgttggaca cgccgatcac gacgatcctg cgggatctgc aggatcgcaa cgtgccgacg     1140

caactgcaca tgttcgcggc cgcgatgcac gccgaggagc tcactttggc ggagctgggt     1200

cggcggtatg gaaccaacgt gggcttcgtt ccgcagtggg ccggcacggc cgagcagatc     1260

gccgaggagt tgatccgcca cttcgacgcc ggcgccgcgg atggcttcat tgtttctccg     1320

gcctttctgc ctggcgcgta tgacgaattc gtcgaccagg tggtgccggt tctgcaggac     1380

cgcggctact tccgcaccga atacgagggc aacaccctgc gcgaccacct gggtctgcgc     1440

gaaccacgac cgttaggaca accttcatgg caggccgcct cagccccgga aaccccggtt     1500

cagaacttga taccggcatc ctcgacacac tga                                  1533


<210>  26
<211>  510
<212>  PRT
<213>  Artificial sequence

<220>
<223>  BclA+ DszA fusion polypeptide

<400>  26

Met Ser Asn Asn Asn Tyr Ser Asn Gly Leu Asn Pro Asp Glu Ser Leu 
1               5                   10                  15      


Ser Ala Ser Ala Phe Asp Pro Asn Leu Val Gly Pro Thr Leu Pro Pro 
            20                  25                  30          


Ile Pro Glu Leu Met Ala Gln Arg Arg Gln Leu His Leu Ala Gly Phe 
        35                  40                  45              


Phe Ser Ala Gly Asn Val Thr His Ala His Gly Ala Trp Arg His Thr 
    50                  55                  60                  


Asp Ala Ser Asn Gly Phe Leu Thr Gly Lys Tyr Tyr Gln His Ile Ala 
65                  70                  75                  80  


Arg Thr Leu Glu Arg Gly Lys Phe Asp Leu Leu Phe Leu Pro Asp Gly 
                85                  90                  95      


Leu Ala Val Glu Asp Ser Tyr Gly Asp Asp Leu Arg Thr Gly Val Gly 
            100                 105                 110         


Leu Gly Gly Gln Gly Ala Val Ala Leu Glu Pro Ala Ser Val Ile Ala 
        115                 120                 125             


Thr Met Ala Ala Val Thr Glu His Leu Gly Leu Gly Ala Thr Ile Ser 
    130                 135                 140                 


Ala Thr Tyr Tyr Pro Pro Tyr His Val Ala Arg Val Phe Ala Thr Leu 
145                 150                 155                 160 


Asp Gln Leu Ser Gly Gly Arg Val Ser Trp Asn Val Val Thr Ser Leu 
                165                 170                 175     


Asn Asp Ala Glu Ala Arg Asn Phe Gly Ile Asp Gln His Leu Glu His 
            180                 185                 190         


Asp Ala Arg Tyr Asp Arg Ala Asp Glu Phe Leu Asp Ala Val Lys Lys 
        195                 200                 205             


Leu Trp Asn Ser Trp Asp Glu Asp Ala Leu Val Leu Asp Lys Ala Ala 
    210                 215                 220                 


Gly Val Phe Ala Asp Pro Thr Lys Val His Tyr Val Asp His His Gly 
225                 230                 235                 240 


Glu Trp Leu Asn Val Arg Gly Pro Leu Gln Val Pro Arg Ser Pro Gln 
                245                 250                 255     


Gly Glu Pro Val Ile Leu Gln Ala Gly Leu Ser Pro Arg Gly Arg Arg 
            260                 265                 270         


Phe Ala Gly Arg Trp Ala Glu Ala Val Phe Ser Val Ala Pro Asp Leu 
        275                 280                 285             


Gly Leu Met Gln Ala Thr Tyr His Asp Ile Lys Ala Gln Val Lys Ala 
    290                 295                 300                 


Ala Gly Arg Asp Pro Asp Gln Thr Lys Ile Phe Thr Ala Val Met Pro 
305                 310                 315                 320 


Val Leu Gly Glu Thr Glu Ala Val Ala Gln Asp Arg Leu Glu Tyr Leu 
                325                 330                 335     


Asn Ser Leu Val His Pro Glu Val Gly Leu Ser Thr Leu Ser Ser His 
            340                 345                 350         


Thr Gly Ile Asn Leu Ala Glu Tyr Pro Leu Asp Thr Pro Ile Thr Thr 
        355                 360                 365             


Ile Leu Arg Asp Leu Gln Asp Arg Asn Val Pro Thr Gln Leu His Met 
    370                 375                 380                 


Phe Ala Ala Ala Met His Ala Glu Glu Leu Thr Leu Ala Glu Leu Gly 
385                 390                 395                 400 


Arg Arg Tyr Gly Thr Asn Val Gly Phe Val Pro Gln Trp Ala Gly Thr 
                405                 410                 415     


Ala Glu Gln Ile Ala Glu Glu Leu Ile Arg His Phe Asp Ala Gly Ala 
            420                 425                 430         


Ala Asp Gly Phe Ile Val Ser Pro Ala Phe Leu Pro Gly Ala Tyr Asp 
        435                 440                 445             


Glu Phe Val Asp Gln Val Val Pro Val Leu Gln Asp Arg Gly Tyr Phe 
    450                 455                 460                 


Arg Thr Glu Tyr Glu Gly Asn Thr Leu Arg Asp His Leu Gly Leu Arg 
465                 470                 475                 480 


Glu Pro Arg Pro Leu Gly Gln Pro Ser Trp Gln Ala Ala Ser Ala Pro 
                485                 490                 495     


Glu Thr Pro Val Gln Asn Leu Ile Pro Ala Ser Ser Thr His 
            500                 505                 510 


<210>  27
<211>  1206
<212>  DNA
<213>  Artificial sequence

<220>
<223>  BclA+ DszB fusion DNA

<400>  27
atgtcaaata ataattattc aaatggatta aaccccgatg aatctttatc agctagtgca       60

tttgacccta atcttgtagg acctacatta ccaccgatac cagagctcat ggcaggccgc      120

ctcagccccg gaaaccccgg ttcagaactt gataccggca tcctcgacac actgacctac      180

agcaactgcc cgatacccaa cgcgctgctc acggcgtggg aatcaggttt cctcgacgcc      240

gccggcatcg aactcgacat cctcagcggc aagcagggaa cggtccactt cacctacgac      300

cagcccgcct acacccgcta tggcggtgag atcccgccac tgctcagcga ggggttgcgg      360

gcacccggac gcacgcgtct actcggcatc accccgatcc tggggcgtca gggcttcttc      420

gtcggcgatc gcagcccgat cacagtggcc gccgaccttg ccggacgccg aataggagtg      480

tcggcttcgg cgattcgcat cctgcgtggc gaactggggg actatctcca gctggatccc      540

tggcggcaga cgctggtagc gctgggctcg tgggaggcgc gcgcgttgtt gcacaccctc      600

gagcacggtg agctcgatgt ggacgacgtc gagctggtgc cgatcaacag tcctggcgtc      660

gatgtccccg ccgagcagtt ggaagacgcc gcgaccctca agggtgcgga cctgtttccc      720

gatgtggccg caggccaggc cgcggtgctg gaccgcggtg aggttgacgc cctgttcagt      780

tggttgccct gggctgcgga gttggaaggc accggcgccc gcccggtggt ggatctcggc      840

ctcgatgagc gcaatgccta tgccagcgtg tggacggtca gcagcgaact ggtcgttgac      900

cggcctgacc tcgttcaacg actggtcgac gcagtcgtcg acgccgggtt gtgggcacgc      960

gaccacggcg atgcggtgac ccgcctgcac gccgcgaacc tgggcgtatc ccccgacgca     1020

gtcggccacg gattcggcgc cgacttccag cagcgtttgg ttccgcgcct ggatcccgac     1080

gcggtcgccc tgctggatcg cacacagcaa ttcctgctca gcaaccagtt gctgcaggag     1140

cccgtcgccc tcgatcagtg ggcggctccg gaatttctga acaccagcct caaccgtcac     1200

cgatag                                                                1206


<210>  28
<211>  401
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BclA+ DszB fusion polypeptide

<400>  28

Met Ser Asn Asn Asn Tyr Ser Asn Gly Leu Asn Pro Asp Glu Ser Leu 
1               5                   10                  15      


Ser Ala Ser Ala Phe Asp Pro Asn Leu Val Gly Pro Thr Leu Pro Pro 
            20                  25                  30          


Ile Pro Glu Leu Met Ala Gly Arg Leu Ser Pro Gly Asn Pro Gly Ser 
        35                  40                  45              


Glu Leu Asp Thr Gly Ile Leu Asp Thr Leu Thr Tyr Ser Asn Cys Pro 
    50                  55                  60                  


Ile Pro Asn Ala Leu Leu Thr Ala Trp Glu Ser Gly Phe Leu Asp Ala 
65                  70                  75                  80  


Ala Gly Ile Glu Leu Asp Ile Leu Ser Gly Lys Gln Gly Thr Val His 
                85                  90                  95      


Phe Thr Tyr Asp Gln Pro Ala Tyr Thr Arg Tyr Gly Gly Glu Ile Pro 
            100                 105                 110         


Pro Leu Leu Ser Glu Gly Leu Arg Ala Pro Gly Arg Thr Arg Leu Leu 
        115                 120                 125             


Gly Ile Thr Pro Ile Leu Gly Arg Gln Gly Phe Phe Val Gly Asp Arg 
    130                 135                 140                 


Ser Pro Ile Thr Val Ala Ala Asp Leu Ala Gly Arg Arg Ile Gly Val 
145                 150                 155                 160 


Ser Ala Ser Ala Ile Arg Ile Leu Arg Gly Glu Leu Gly Asp Tyr Leu 
                165                 170                 175     


Gln Leu Asp Pro Trp Arg Gln Thr Leu Val Ala Leu Gly Ser Trp Glu 
            180                 185                 190         


Ala Arg Ala Leu Leu His Thr Leu Glu His Gly Glu Leu Asp Val Asp 
        195                 200                 205             


Asp Val Glu Leu Val Pro Ile Asn Ser Pro Gly Val Asp Val Pro Ala 
    210                 215                 220                 


Glu Gln Leu Glu Asp Ala Ala Thr Leu Lys Gly Ala Asp Leu Phe Pro 
225                 230                 235                 240 


Asp Val Ala Ala Gly Gln Ala Ala Val Leu Asp Arg Gly Glu Val Asp 
                245                 250                 255     


Ala Leu Phe Ser Trp Leu Pro Trp Ala Ala Glu Leu Glu Gly Thr Gly 
            260                 265                 270         


Ala Arg Pro Val Val Asp Leu Gly Leu Asp Glu Arg Asn Ala Tyr Ala 
        275                 280                 285             


Ser Val Trp Thr Val Ser Ser Glu Leu Val Val Asp Arg Pro Asp Leu 
    290                 295                 300                 


Val Gln Arg Leu Val Asp Ala Val Val Asp Ala Gly Leu Trp Ala Arg 
305                 310                 315                 320 


Asp His Gly Asp Ala Val Thr Arg Leu His Ala Ala Asn Leu Gly Val 
                325                 330                 335     


Ser Pro Asp Ala Val Gly His Gly Phe Gly Ala Asp Phe Gln Gln Arg 
            340                 345                 350         


Leu Val Pro Arg Leu Asp Pro Asp Ala Val Ala Leu Leu Asp Arg Thr 
        355                 360                 365             


Gln Gln Phe Leu Leu Ser Asn Gln Leu Leu Gln Glu Pro Val Ala Leu 
    370                 375                 380                 


Asp Gln Trp Ala Ala Pro Glu Phe Leu Asn Thr Ser Leu Asn Arg His 
385                 390                 395                 400 


Arg 
    


<210>  29
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer sequence for BclB

<400>  29
gctacatatg ttgaaacaga atgacaaatt atg                                    33


<210>  30
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer sequence for BclB

<400>  30
gtcagagctc tgggggaaga accgggaagg tagg                                   34


<210>  31
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer sequence for DszC

<400>  31
gtcagagctc atgactctgt ccgttgaaaa gcagcac                                37


<210>  32
<211>  39
<212>  DNA
<213>  Artificial sequence

<220>
<223>  reverse primer sequence for DszC

<400>  32
ccgcccaagc ttctaggagg tgaagccggg aatcgggta                              39


<210>  33
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer sequence for BclA

<400>  33
gctacatatg gctacatatg tcaaataata attattc                                37


<210>  34
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer sequence for BclA

<400>  34
gtcagagctc tgacgagctc tggtatcggt ggtaatg                                37


<210>  35
<211>  1047
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  full length BclB DNA

<400>  35
ttgaaacaga atgacaaatt atggttggat aaaggcataa taggcccaga aaatattgga       60

cctaccttcc cggttcttcc cccaattcat attccaacag gaataacggg agcaaccggt      120

gcaacaggaa taacgggagc aacaggtccg acgggaacaa caggagcgac cggtgcgaca      180

ggaataacgg gagtgaccgg tgcaacagga ataacgggag tgaccggtgc aacaggaata      240

acgggagtga ccggtgcaac aggaataacg ggagtgaccg gtgcaacagg aataacggga      300

gtgacaggtc cgacgggaat aacgggagca acaggtccga cgggaacaac aggagtaacc      360

ggtccaacgg gagacacagg tctagcagga gcaacgggcc cgacaggagc aacaggtcta      420

gcaggagcaa cgggcccaac aggagatact ggagcaacgg gcccgacagg agcaacaggt      480

ctagcaggag caacaggccc gacaggagca acaggtctaa cgggagcaac tggtgcaaca      540

ggagcaactg gtggcggagc tattattcca ttcgcttccg gtacaacacc agctctgtta      600

gttaatgcgg tattagctaa tacagggact cttcttggat ttggatttag tcagcctggc      660

atagctccag gtgtgggagg aactctcaca atactaccag gtgttgtagg tgattatgca      720

tttgtagcac cacgtgatgg aattatcact tcattagcag gattctttag tgcaacagcg      780

gcgttagctc cattgacacc tgttcaaatt cagatgcaaa tatttatcgc acctgctgca      840

agtaatacgt ttacaccagt agcgccacct ctattattaa caccagcatt accagcaata      900

gcaattggta ctacagcaac aggaatccaa gcttataatg ttccagtagt tgctggggat      960

aaaatattag tatatgtttc attaacagga gctagtccaa tagctgcagt tgccggattt     1020

gtaagcgcag gtcttaatat cgtctaa                                         1047


<210>  36
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BclB protein

<400>  36

Ala Thr Gly Ala Thr Gly Ile Thr Gly Val Thr Gly Ala Thr Gly Ile 
1               5                   10                  15      


Thr Gly Val Thr Gly Ala Thr Gly Ile Thr Gly Val Thr Gly Ala Thr 
            20                  25                  30          


Gly Ile Thr Gly Val Thr Gly Ala Thr Gly Ile Thr Gly Val Thr Gly 
        35                  40                  45              


Pro Thr Gly Ile Thr Gly Ala Thr Gly Pro Thr Gly Thr Thr Gly Val 
    50                  55                  60                  


Thr Gly Pro Thr Gly Asp Thr Gly Leu Ala Gly Ala Thr Gly Pro Thr 
65                  70                  75                  80  


Gly Ala Thr Gly Leu Ala Gly Ala Thr Gly Pro Thr Gly Asp Thr Gly 
                85                  90                  95      


Ala Thr Gly Pro Thr Gly Ala Thr Gly Leu Ala Gly Ala Thr Gly Pro 
            100                 105                 110         


Thr Gly Ala Thr Gly Leu Thr Gly Ala Thr Gly Ala Thr Gly Ala Thr 
        115                 120                 125             


Gly Gly Gly Ala Ile Ile Pro Phe Ala Ser Gly Thr Thr Pro Ala Leu 
    130                 135                 140                 


Leu Val Asn Ala Val Leu Ala Asn Thr Gly Thr Leu Leu Gly Phe Gly 
145                 150                 155                 160 


Phe Ser Gln Pro Gly Ile Ala Pro Gly Val Gly Gly Thr Leu Thr Ile 
                165                 170                 175     


Leu Pro Gly Val Val Gly Asp Tyr Ala Phe Val Ala Pro Arg Asp Gly 
            180                 185                 190         


Ile Ile Thr Ser Leu Ala Gly Phe Phe Ser Ala Thr Ala Ala Leu Ala 
        195                 200                 205             


Pro Leu Thr Pro Val Gln Ile Gln Met Gln Ile Phe Ile Ala Pro Ala 
    210                 215                 220                 


Ala Ser Asn Thr Phe Thr Pro Val Ala Pro Pro Leu Leu Leu Thr Pro 
225                 230                 235                 240 


Ala Leu Pro Ala Ile Ala Ile Gly Thr Thr Ala Thr Gly Ile Gln Ala 
                245                 250                 255     


Tyr Asn Val Pro Val Val Ala Gly Asp Lys Ile Leu Val Tyr Val Ser 
            260                 265                 270         


Leu Thr Gly Ala Ser Pro Ile Ala Ala Val Ala Gly Phe Val Ser Ala 
        275                 280                 285             


Gly Leu Asn Ile Val 
    290             


<210>  37
<211>  762
<212>  DNA
<213>  Artificial sequence

<220>
<223>  full length BclA DNA

<400>  37
atgtcaaata ataattattc aaatggatta aaccccgatg aatctttatc agctagtgca       60

tttgacccta atcttgtagg acctacatta ccaccgatac caccatttac ccttcctacc      120

ggaccaactg ggccgactgg accgactggg ccgactgggc caactggacc aactgggccg      180

actgggccaa ctggaccaac tggaccaact gggccaactg gaccaactgg gccaactggg      240

ccaactggag acactggtac tactggacca actgggccaa ctggaccaac tggaccaact      300

gggccaactg gtgctaccgg actgactgga ccgactggac cgactgggcc atccggacta      360

ggacttccag caggactata tgcatttaac tccggtggga tttctttaga tttaggaatt      420

aatgatccag taccatttaa tactgttgga tctcagtttg gtacagcaat ttctcaatta      480

gatgctgata ctttcgtaat tagtgaaact ggattctata aaattactgt tatcgctaat      540

actgcaacag caagtgtatt aggaggtctt acaatccaag tgaatggagt acctgtacca      600

ggtactggat caagtttgat ttcactcgga gcacctatcg ttattcaagc aattacgcaa      660

attacgacaa ctccatcatt agttgaagta attgttacag ggcttggact atcactagct      720

cttggcacga gtgcatccat tattattgaa aaagttgctt aa                         762


<210>  38
<211>  253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BclA protein

<400>  38

Met Ser Asn Asn Asn Tyr Ser Asn Gly Leu Asn Pro Asp Glu Ser Leu 
1               5                   10                  15      


Ser Ala Ser Ala Phe Asp Pro Asn Leu Val Gly Pro Thr Leu Pro Pro 
            20                  25                  30          


Ile Pro Pro Phe Thr Leu Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro 
        35                  40                  45              


Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr 
    50                  55                  60                  


Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly 
65                  70                  75                  80  


Pro Thr Gly Asp Thr Gly Thr Thr Gly Pro Thr Gly Pro Thr Gly Pro 
                85                  90                  95      


Thr Gly Pro Thr Gly Pro Thr Gly Ala Thr Gly Leu Thr Gly Pro Thr 
            100                 105                 110         


Gly Pro Thr Gly Pro Ser Gly Leu Gly Leu Pro Ala Gly Leu Tyr Ala 
        115                 120                 125             


Phe Asn Ser Gly Gly Ile Ser Leu Asp Leu Gly Ile Asn Asp Pro Val 
    130                 135                 140                 


Pro Phe Asn Thr Val Gly Ser Gln Phe Gly Thr Ala Ile Ser Gln Leu 
145                 150                 155                 160 


Asp Ala Asp Thr Phe Val Ile Ser Glu Thr Gly Phe Tyr Lys Ile Thr 
                165                 170                 175     


Val Ile Ala Asn Thr Ala Thr Ala Ser Val Leu Gly Gly Leu Thr Ile 
            180                 185                 190         


Gln Val Asn Gly Val Pro Val Pro Gly Thr Gly Ser Ser Leu Ile Ser 
        195                 200                 205             


Leu Gly Ala Pro Ile Val Ile Gln Ala Ile Thr Gln Ile Thr Thr Thr 
    210                 215                 220                 


Pro Ser Leu Val Glu Val Ile Val Thr Gly Leu Gly Leu Ser Leu Ala 
225                 230                 235                 240 


Leu Gly Thr Ser Ala Ser Ile Ile Ile Glu Lys Val Ala 
                245                 250             


