                         SEQUENCE LISTING

<110>  DANISCO
 
<120>  METHODS AND COMPOSITIONS FOR EFFICIENT GENETIC MODIFICATIONS OF 
       BACILLUS LICHENIFORMIS STRAINS

<130>  NB41109-WO-PCT

<150>  US62/549,363
<151>  2017-08-23

<160>  71    

<170>  PatentIn version 3.5

<210>  1
<211>  1320
<212>  DNA
<213>  Bacillus licheniformis

<400>  1
ttgtttttac acggtactag cagacaaaat gaaagagggc acctcgaaat cggcggtgtc       60

gatgttctat cattggcaga aagatacgga acacctcttt atgtatacga tgtcgcgctg      120

attagagagc gcgcccgaaa attccagaag gcattcaagg aagccggttt aaaagcgcag      180

gtagcgtatg caagcaaggc gttttcatcg gttgccatga ttcagcttgc cgaacaagag      240

gggctgtctc tggatgtggt atcgggagga gagcttttca ctgcgatcaa agcagggttc      300

ccagctgagc ggattcattt tcacggaaac aataagagcc ctgaagaact agccatggcg      360

ctggagcatc aaatcggctg catcgtgctc gataactttc acgagatcgc cattacagaa      420

gatctttgca agcgatcagg acaaactgta gacgttttgc tcagaatcac tccgggagtt      480

gaagcgcaca cgcacgatta tattacgacg gggcaggaag attccaaatt cggttttgat      540

ctgcataatg gacaggtcga acaagccatc gaacaagtcc tccgctcgtc tgcgtttaag      600

ctcctcggcg tgcactgcca catcggttcg caaatttttg atacggcagg atttgtcctt      660

gcagcagaca agattttcga gaagcttgcg gaatggcggg agacttactc tttcattccg      720

gaagtgctca atcttggcgg gggcttcggc atccgctata caaaagacga cgagccgctt      780

gcagctgatg tttatgttga aaaaatcatc gaggcggtca aagcaaatgc cgagcatttc      840

ggctttgaca tccctgagat ttggatcgaa ccaggccggt ctctcgtcgg tgatgcgggg      900

actacgctgt acacgatcgg ttctcaaaaa gaggtgccgg gcattcgcaa atatgtagcc      960

atcgacggcg gcatgagcga taatatcagg ccggcgcttt atgaggcaaa atatgaagca     1020

gccgtcgcca acaggatgaa cgatgcttgt catgataccg catcaatcgc aggaaaatgc     1080

tgcgaaagcg gagatatgct gatttgggat ttggaaatcc ccgaagttcg cgacggagat     1140

gtgctcgccg ttttctgcac cggtgcgtac ggctacagca tggccaacaa ctacaaccgc     1200

attccgcgcc cggccgtcgt ctttgtcgag gacggggaag cgcagctcgt cattcagaga     1260

gagacgtatg aggatatcgt caagctggat ctgccgctga aatcgaaagt caaacaataa     1320


<210>  2
<211>  439
<212>  PRT
<213>  Bacillus licheniformis

<400>  2

Met Phe Leu His Gly Thr Ser Arg Gln Asn Glu Arg Gly His Leu Glu 
1               5                   10                  15      


Ile Gly Gly Val Asp Val Leu Ser Leu Ala Glu Arg Tyr Gly Thr Pro 
            20                  25                  30          


Leu Tyr Val Tyr Asp Val Ala Leu Ile Arg Glu Arg Ala Arg Lys Phe 
        35                  40                  45              


Gln Lys Ala Phe Lys Glu Ala Gly Leu Lys Ala Gln Val Ala Tyr Ala 
    50                  55                  60                  


Ser Lys Ala Phe Ser Ser Val Ala Met Ile Gln Leu Ala Glu Gln Glu 
65                  70                  75                  80  


Gly Leu Ser Leu Asp Val Val Ser Gly Gly Glu Leu Phe Thr Ala Ile 
                85                  90                  95      


Lys Ala Gly Phe Pro Ala Glu Arg Ile His Phe His Gly Asn Asn Lys 
            100                 105                 110         


Ser Pro Glu Glu Leu Ala Met Ala Leu Glu His Gln Ile Gly Cys Ile 
        115                 120                 125             


Val Leu Asp Asn Phe His Glu Ile Ala Ile Thr Glu Asp Leu Cys Lys 
    130                 135                 140                 


Arg Ser Gly Gln Thr Val Asp Val Leu Leu Arg Ile Thr Pro Gly Val 
145                 150                 155                 160 


Glu Ala His Thr His Asp Tyr Ile Thr Thr Gly Gln Glu Asp Ser Lys 
                165                 170                 175     


Phe Gly Phe Asp Leu His Asn Gly Gln Val Glu Gln Ala Ile Glu Gln 
            180                 185                 190         


Val Leu Arg Ser Ser Ala Phe Lys Leu Leu Gly Val His Cys His Ile 
        195                 200                 205             


Gly Ser Gln Ile Phe Asp Thr Ala Gly Phe Val Leu Ala Ala Asp Lys 
    210                 215                 220                 


Ile Phe Glu Lys Leu Ala Glu Trp Arg Glu Thr Tyr Ser Phe Ile Pro 
225                 230                 235                 240 


Glu Val Leu Asn Leu Gly Gly Gly Phe Gly Ile Arg Tyr Thr Lys Asp 
                245                 250                 255     


Asp Glu Pro Leu Ala Ala Asp Val Tyr Val Glu Lys Ile Ile Glu Ala 
            260                 265                 270         


Val Lys Ala Asn Ala Glu His Phe Gly Phe Asp Ile Pro Glu Ile Trp 
        275                 280                 285             


Ile Glu Pro Gly Arg Ser Leu Val Gly Asp Ala Gly Thr Thr Leu Tyr 
    290                 295                 300                 


Thr Ile Gly Ser Gln Lys Glu Val Pro Gly Ile Arg Lys Tyr Val Ala 
305                 310                 315                 320 


Ile Asp Gly Gly Met Ser Asp Asn Ile Arg Pro Ala Leu Tyr Glu Ala 
                325                 330                 335     


Lys Tyr Glu Ala Ala Val Ala Asn Arg Met Asn Asp Ala Cys His Asp 
            340                 345                 350         


Thr Ala Ser Ile Ala Gly Lys Cys Cys Glu Ser Gly Asp Met Leu Ile 
        355                 360                 365             


Trp Asp Leu Glu Ile Pro Glu Val Arg Asp Gly Asp Val Leu Ala Val 
    370                 375                 380                 


Phe Cys Thr Gly Ala Tyr Gly Tyr Ser Met Ala Asn Asn Tyr Asn Arg 
385                 390                 395                 400 


Ile Pro Arg Pro Ala Val Val Phe Val Glu Asp Gly Glu Ala Gln Leu 
                405                 410                 415     


Val Ile Gln Arg Glu Thr Tyr Glu Asp Ile Val Lys Leu Asp Leu Pro 
            420                 425                 430         


Leu Lys Ser Lys Val Lys Gln 
        435                 


<210>  3
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  3
gacgcggccg ccgatcaggt cattgcgaac g                                      31


<210>  4
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  4
tgctcctgca ggcggcttca tgatagtgcg at                                     32


<210>  5
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  5
ccgcctgcag gagcacgaaa aacacttccc g                                      31


<210>  6
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  6
atcctcgaga tgggttccgg catacttgt                                         29


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  7
ccaagcgtca tcatcacacc                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  8
actcttgcgt tcttctccgt                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  9
ccaagcgtca tcatcacacc                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  10
cgccgaaaca cctccttttt                                                   20


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  11
tttaagcccg acgatcatcc a                                                 21


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  12
gtgttccgta tctttctgcc aa                                                22


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  13
ctacagcatg gccaacaact a                                                 21


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  14
atttcagaaa agtcaggcgg g                                                 21


<210>  15
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  15
gtgatgaaaa ggacccaggt ggcacttttc ggggaaatgt gcgcggaacg ctttgacctt       60

ggcgtacgga aggtc                                                        75


<210>  16
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  16
ccgaattttt aacaagtacc attttcccta tattttcttc caaaagaaaa gctgaattaa       60

aaatggagac ccccctctta g                                                 81


<210>  17
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  17
ggaagaaaat atagggaaaa tggtacttgt taaaaattcg gaatatttat acaatatcat       60

atgacagaat agtcttttaa gtaagtctac                                        90


<210>  18
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  18
aaaataaaaa aacggatttc cttcaggaaa tccgtcctct ctgctctttc attgctgcac       60

ccatactgaa actg                                                         74


<210>  19
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  19
gagcagagag gacggatttc ctgaaggaaa tccgtttttt tattttcaag cacgaaaaac       60

acttcccggt gatc                                                         74


<210>  20
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  20
gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg gtacagcaat       60

tctgcctgaa gcc                                                          73


<210>  21
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  21
gtgatgaaaa ggacccaggt ggcacttttc ggggaaatgt gcgcggaacg ttggggacaa       60

ttgcttgccc agctgaaac                                                    79


<210>  22
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  22
gagccgaaaa gctgagattt tcgcgactat aatatcttaa catttaatcg tgatgtc          57


<210>  23
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  23
gacatcacga ttaaatgtta agatattata gtcgcgaaaa tctcagcttt tcggctc          57


<210>  24
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  24
ccgaattttt aacaagtacc attttcccta tattttcttc caaaagaaaa gctgaattaa       60

aaatggagac ccccctctta g                                                 81


<210>  25
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  25
gagcagagag gacggatttc ctgaaggaaa tccgtttttt tattttgctt tgagagcggc       60

aacgaagttc                                                              70


<210>  26
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  26
gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg ggccgtattg       60

agcttcggca tctcgg                                                       76


<210>  27
<211>  1578
<212>  DNA
<213>  Bacillus licheniformis

<400>  27
atgtttcgag tattggtctc agataaaatg tccagcgacg gcctcaaacc attaatggaa       60

gcagatttta ttgaaattgt agaaaagaat gttgcggaag cggaagacga gcttcatacg      120

tttgacgcgc tcttggtgcg gagcgccacg aaggtaaccg aagagctgtt taaaaagatg      180

acttcgctga aaatcgtcgc cagagcaggt gtcggcgtcg acaatatcga tattgacgag      240

gcgacaaaac acggtgttat cgtcgtaaac gcgccaaacg ggaatacaat ttcaaccgct      300

gaacatacct ttgcaatgtt ttcagcgtta atgagacata ttccgcaggc aaacatctcc      360

gtgaaatcaa gggagtggaa tcgttcggct tacgtcggtt cagagcttta cggaaaaacg      420

ctcggcatca tcggaatggg ccgcatcgga agcgaaatcg cgagccgcgc aaaagcattc      480

ggtatgaccg ttcatgtatt tgacccgttc ctgacccaag aaagggcaag caagctcggc      540

gttaacgcga acagctttga agaagttctg gcatgcgccg acatcattac ggttcatacc      600

ccgctcacga aagaaacgaa gggacttttg aacaaagaaa ccatcgcaaa aacgaaaaaa      660

ggcgttcgtc tcgttaactg tgcaagaggc ggcatcatcg atgaagcagc gcttttggaa      720

gctctggaaa gcggacatgt cgctggcgct gccttggatg tattcgaagt cgagcctccg      780

gtcgattcaa aactgatcga tcatccgctt gtagtcgcga ctcctcactt gggcgcctca      840

acaaaagaag cccagctgaa tgtcgctgca caagtgtccg aagaagtcct tcagtatgcg      900

caaggaaacc ctgtgatgtc cgcgatcaac cttccggcca tgacaaagga ttcattcgaa      960

aaaatccagc cttatcatca gtttgccaat acgatcggaa accttgtgtc tcagtgcatg     1020

aatgagcctg ttcaagatgt agccatccaa tatgaaggct ccatcgccaa acttgaaacg     1080

tcatttatta cgaaaagcct tttggccgga tttctgaagc cgagggtcgc ggctaccgtt     1140

aacgaagtga atgccggcac cgttgcgaaa gagcgcggca tcagcttcag cgaaaaaatt     1200

tcttccaatg agtcaggcta tgaaaactgc atctctgtga ctgtcacggg agatgtaaca     1260

acattctctt taagagcgac gtacattccg cacttcggcg gacgcatcgt tgccttaaac     1320

ggctttgata ttgattttta tccggctgga caccttgtct acattcacca ccaggataaa     1380

ccaggggcta tcggccatgt cggacgaatt ttaggagacc atgacatcaa tatcgccact     1440

atgcaggtag gccgaaaaga aaaaggcgga gaagcgatca tgatgctttc ctttgaccgc     1500

caccttgagg acgatatttt agctgagctg aaaaacatcc cggatatcgt gtctgttaaa     1560

gccatcgacc ttccttaa                                                   1578


<210>  28
<211>  525
<212>  PRT
<213>  Bacillus licheniformis

<400>  28

Met Phe Arg Val Leu Val Ser Asp Lys Met Ser Ser Asp Gly Leu Lys 
1               5                   10                  15      


Pro Leu Met Glu Ala Asp Phe Ile Glu Ile Val Glu Lys Asn Val Ala 
            20                  25                  30          


Glu Ala Glu Asp Glu Leu His Thr Phe Asp Ala Leu Leu Val Arg Ser 
        35                  40                  45              


Ala Thr Lys Val Thr Glu Glu Leu Phe Lys Lys Met Thr Ser Leu Lys 
    50                  55                  60                  


Ile Val Ala Arg Ala Gly Val Gly Val Asp Asn Ile Asp Ile Asp Glu 
65                  70                  75                  80  


Ala Thr Lys His Gly Val Ile Val Val Asn Ala Pro Asn Gly Asn Thr 
                85                  90                  95      


Ile Ser Thr Ala Glu His Thr Phe Ala Met Phe Ser Ala Leu Met Arg 
            100                 105                 110         


His Ile Pro Gln Ala Asn Ile Ser Val Lys Ser Arg Glu Trp Asn Arg 
        115                 120                 125             


Ser Ala Tyr Val Gly Ser Glu Leu Tyr Gly Lys Thr Leu Gly Ile Ile 
    130                 135                 140                 


Gly Met Gly Arg Ile Gly Ser Glu Ile Ala Ser Arg Ala Lys Ala Phe 
145                 150                 155                 160 


Gly Met Thr Val His Val Phe Asp Pro Phe Leu Thr Gln Glu Arg Ala 
                165                 170                 175     


Ser Lys Leu Gly Val Asn Ala Asn Ser Phe Glu Glu Val Leu Ala Cys 
            180                 185                 190         


Ala Asp Ile Ile Thr Val His Thr Pro Leu Thr Lys Glu Thr Lys Gly 
        195                 200                 205             


Leu Leu Asn Lys Glu Thr Ile Ala Lys Thr Lys Lys Gly Val Arg Leu 
    210                 215                 220                 


Val Asn Cys Ala Arg Gly Gly Ile Ile Asp Glu Ala Ala Leu Leu Glu 
225                 230                 235                 240 


Ala Leu Glu Ser Gly His Val Ala Gly Ala Ala Leu Asp Val Phe Glu 
                245                 250                 255     


Val Glu Pro Pro Val Asp Ser Lys Leu Ile Asp His Pro Leu Val Val 
            260                 265                 270         


Ala Thr Pro His Leu Gly Ala Ser Thr Lys Glu Ala Gln Leu Asn Val 
        275                 280                 285             


Ala Ala Gln Val Ser Glu Glu Val Leu Gln Tyr Ala Gln Gly Asn Pro 
    290                 295                 300                 


Val Met Ser Ala Ile Asn Leu Pro Ala Met Thr Lys Asp Ser Phe Glu 
305                 310                 315                 320 


Lys Ile Gln Pro Tyr His Gln Phe Ala Asn Thr Ile Gly Asn Leu Val 
                325                 330                 335     


Ser Gln Cys Met Asn Glu Pro Val Gln Asp Val Ala Ile Gln Tyr Glu 
            340                 345                 350         


Gly Ser Ile Ala Lys Leu Glu Thr Ser Phe Ile Thr Lys Ser Leu Leu 
        355                 360                 365             


Ala Gly Phe Leu Lys Pro Arg Val Ala Ala Thr Val Asn Glu Val Asn 
    370                 375                 380                 


Ala Gly Thr Val Ala Lys Glu Arg Gly Ile Ser Phe Ser Glu Lys Ile 
385                 390                 395                 400 


Ser Ser Asn Glu Ser Gly Tyr Glu Asn Cys Ile Ser Val Thr Val Thr 
                405                 410                 415     


Gly Asp Val Thr Thr Phe Ser Leu Arg Ala Thr Tyr Ile Pro His Phe 
            420                 425                 430         


Gly Gly Arg Ile Val Ala Leu Asn Gly Phe Asp Ile Asp Phe Tyr Pro 
        435                 440                 445             


Ala Gly His Leu Val Tyr Ile His His Gln Asp Lys Pro Gly Ala Ile 
    450                 455                 460                 


Gly His Val Gly Arg Ile Leu Gly Asp His Asp Ile Asn Ile Ala Thr 
465                 470                 475                 480 


Met Gln Val Gly Arg Lys Glu Lys Gly Gly Glu Ala Ile Met Met Leu 
                485                 490                 495     


Ser Phe Asp Arg His Leu Glu Asp Asp Ile Leu Ala Glu Leu Lys Asn 
            500                 505                 510         


Ile Pro Asp Ile Val Ser Val Lys Ala Ile Asp Leu Pro 
        515                 520                 525 


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  29
tacagcattt cgcggccgta c                                                 21


<210>  30
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  30
catagatcgg ggtgaagatg tcatgatctt attttgcctc ataaagcgcc ggcctg           56


<210>  31
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  31
caggccggcg ctttatgagg caaaataaga tcatgacatc ttcaccccga tctatg           56


<210>  32
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  32
attagcccgg tcgctgttct atgcatttaa gagacccgct aagaagtaca ta               52


<210>  33
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  33
tatgtacttc ttagcgggtc tcttaaatgc atagaacagc gaccgggcta at               52


<210>  34
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  34
gttcagacac acaatatcag cag                                               23


<210>  35
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  35
ggaaatgtgc gcggaacccc tatttgcatt tcgcggccgt acgggtcaat cg               52


<210>  36
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  36
atactgacat agatcggggt gaagatgtca tgatcttttt aacaaaaaac ac               52


<210>  37
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  37
gtgttttttg ttaaaaagat catgacatct tcaccccgat ctatgtcagt at               52


<210>  38
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  38
attagcccgg tcgctgttct atgcatttaa gagacccgct aagaagtaca ta               52


<210>  39
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  39
tatgtacttc ttagcgggtc tcttaaatgc atagaacagc gaccgggcta at               52


<210>  40
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  40
agatgcgtaa ggagaaaata ccgcatggag ggcatttgac ttgaagagaa aa               52


<210>  41
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  41
gtcaatcgtt gacgaatgaa gg                                                22


<210>  42
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  42
ggcatttgac ttgaagagaa aattctc                                           27


<210>  43
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  43
gatgcggccg catcgaaacg gccatcacaa g                                      31


<210>  44
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  44
ggatgtcctg cagggtcaat gagtttcacg cggaa                                  35


<210>  45
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  45
attgaccctg caggacatcc cggatatcgt gtct                                   34


<210>  46
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  46
atgctcgagc ggaagcctca gagtggatt                                         29


<210>  47
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  47
cggggaaatg tgcgcggaac ccctatttgc tctgaccaaa gactcctgct tc               52


<210>  48
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  48
gtcagctgtt tatcagcgag agctcggaaa aagtgagttt accgaaatat c                51


<210>  49
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  49
catattccgc attcgcaatg cctaccgcat actaaaaacc gcacattcac ag               52


<210>  50
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  50
cagatgcgta aggagaaaat accgcatatg ctgatcactc cccagttaat cg               52


<210>  51
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  51
gatatttcgg taaactcact ttttccgagc tctcgctgat aaacagctga c                51


<210>  52
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  52
ctgtgaatgt gcggttttta gtatgcggta ggcattgcga atgcggaata tg               52


<210>  53
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  53
gcttcaatcg ttgaacgctg gc                                                22


<210>  54
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  54
cagttaatcg aacagatcag atctc                                             25


<210>  55
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  55
ggaaatgtgc gcggaacccc tatttggtct atgtgaagct gtcgccgaac gt               52


<210>  56
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  56
aacaatatgg cccgtttgtt gaactgggat taataaaaaa taaaaataat cc               52


<210>  57
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  57
ggattatttt tattttttat taatcccagt tcaacaaacg ggccatattg tt               52


<210>  58
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  58
taaaagtcaa cccgactttt tttatagccg cgcataaaaa aagaccattc ct               52


<210>  59
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  59
aggaatggtc tttttttatg cgcggctata aaaaaagtcg ggttgacttt ta               52


<210>  60
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  60
agatgcgtaa ggagaaaata ccgcatgact catctcctta ctcgtgtaat cc               52


<210>  61
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  61
cattaccgac atcgctcttg c                                                 21


<210>  62
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  62
ctccttactc gtgtaatccg cattc                                             25


<210>  63
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  63
gcttcaatcg ttgaacgctg gc                                                22


<210>  64
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  64
ctgacggaaa attggtgcag ac                                                22


<210>  65
<211>  720
<212>  DNA
<213>  Bacillus licheniformis

<400>  65
atgaacaata cgccgcccat catcgcgctt gattttgcat cagcacagga aacatatgcg       60

tttttggacc ggtttcaagg agaagagctg ttcgttaagg tgggcatgga gcttttttat      120

caggaaggac ctgccatcct tgaaaatctg caggagcgcg gctgccgcat tttcctcgat      180

ctaaagtgcc acgacattcc aacgaccgta tacaaggcga tgaaaaggct ggcgggattt      240

ggcgtcagtc ttgtcaatgt gcacgcagca ggcggaaagc aaatgatgga gtcggcgctt      300

gaaggccttg aagcggggac tccggcaggc caaaaacgcc cttcattaat tgccgtgact      360

cagctgacaa gcacgtcctc agaaatgctt cagcgtgagc tcctgattga aacgccgctt      420

ctcgatacag ttgtccacta cagccggctg gctgaagaaa gcggtcttga cggcgtcgtc      480

tgctccgttc atgaagccga acacatttac cgggctgtgt ctgtagattt tctgaccgtg      540

acacccggaa ttcgcatggc agatgacaaa aacaacgacc aggtccgggt ggcgacgcct      600

ggctacgcaa gggaaaaagg cgtgtcggcg atcgttgtcg gccgttcgat tacccaggcg      660

gaagatcctg tgtcagccta cagaagaata ggacatgagt gggagggaac caaggcatga      720


<210>  66
<211>  239
<212>  PRT
<213>  Bacillus licheniformis

<400>  66

Met Asn Asn Thr Pro Pro Ile Ile Ala Leu Asp Phe Ala Ser Ala Gln 
1               5                   10                  15      


Glu Thr Tyr Ala Phe Leu Asp Arg Phe Gln Gly Glu Glu Leu Phe Val 
            20                  25                  30          


Lys Val Gly Met Glu Leu Phe Tyr Gln Glu Gly Pro Ala Ile Leu Glu 
        35                  40                  45              


Asn Leu Gln Glu Arg Gly Cys Arg Ile Phe Leu Asp Leu Lys Cys His 
    50                  55                  60                  


Asp Ile Pro Thr Thr Val Tyr Lys Ala Met Lys Arg Leu Ala Gly Phe 
65                  70                  75                  80  


Gly Val Ser Leu Val Asn Val His Ala Ala Gly Gly Lys Gln Met Met 
                85                  90                  95      


Glu Ser Ala Leu Glu Gly Leu Glu Ala Gly Thr Pro Ala Gly Gln Lys 
            100                 105                 110         


Arg Pro Ser Leu Ile Ala Val Thr Gln Leu Thr Ser Thr Ser Ser Glu 
        115                 120                 125             


Met Leu Gln Arg Glu Leu Leu Ile Glu Thr Pro Leu Leu Asp Thr Val 
    130                 135                 140                 


Val His Tyr Ser Arg Leu Ala Glu Glu Ser Gly Leu Asp Gly Val Val 
145                 150                 155                 160 


Cys Ser Val His Glu Ala Glu His Ile Tyr Arg Ala Val Ser Val Asp 
                165                 170                 175     


Phe Leu Thr Val Thr Pro Gly Ile Arg Met Ala Asp Asp Lys Asn Asn 
            180                 185                 190         


Asp Gln Val Arg Val Ala Thr Pro Gly Tyr Ala Arg Glu Lys Gly Val 
        195                 200                 205             


Ser Ala Ile Val Val Gly Arg Ser Ile Thr Gln Ala Glu Asp Pro Val 
    210                 215                 220                 


Ser Ala Tyr Arg Arg Ile Gly His Glu Trp Glu Gly Thr Lys Ala 
225                 230                 235                 


<210>  67
<211>  579
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  encodes comK protein

<400>  67
atgagcacag aggatatgac aaaggatacg tatgaagtaa acagttcgac aatggctgtc       60

ctgcctctgg gtgaggggga gaaatccgcc tcaaaaatac ttgagaccga caggactttc      120

cgcgtcaata tgaagccgtt tcaaattatc gaaagaagct gccgctattt cggatcgagc      180

tatgcgggaa gaaaagcggg cacatatgaa gtcattaaag tttcccataa accgccgatc      240

atggtggatc actcaaacaa catttttctt ttccccacat tttcctcaac tcgtcctcag      300

tgcgggtggc tttcccatgc gcatgttcac gagttttgcg cggcaaagta tgacaacacg      360

tttgtcacgt ttgtcaacgg ggaaacgctg gagctgcccg tatccatctc atctttcgaa      420

aaccaggttt accgaacggc atggctgaga acaaaattta tcgacaggat tgaaggaaac      480

cccatgcaga agaaacagga atttatgctc tatccgaaag aagaccggaa tcagctgata      540

tacgaattca tcctcaggga gctgaaaaag cgctattga                             579


<210>  68
<211>  192
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  comK protein

<400>  68

Met Ser Thr Glu Asp Met Thr Lys Asp Thr Tyr Glu Val Asn Ser Ser 
1               5                   10                  15      


Thr Met Ala Val Leu Pro Leu Gly Glu Gly Glu Lys Ser Ala Ser Lys 
            20                  25                  30          


Ile Leu Glu Thr Asp Arg Thr Phe Arg Val Asn Met Lys Pro Phe Gln 
        35                  40                  45              


Ile Ile Glu Arg Ser Cys Arg Tyr Phe Gly Ser Ser Tyr Ala Gly Arg 
    50                  55                  60                  


Lys Ala Gly Thr Tyr Glu Val Ile Lys Val Ser His Lys Pro Pro Ile 
65                  70                  75                  80  


Met Val Asp His Ser Asn Asn Ile Phe Leu Phe Pro Thr Phe Ser Ser 
                85                  90                  95      


Thr Arg Pro Gln Cys Gly Trp Leu Ser His Ala His Val His Glu Phe 
            100                 105                 110         


Cys Ala Ala Lys Tyr Asp Asn Thr Phe Val Thr Phe Val Asn Gly Glu 
        115                 120                 125             


Thr Leu Glu Leu Pro Val Ser Ile Ser Ser Phe Glu Asn Gln Val Tyr 
    130                 135                 140                 


Arg Thr Ala Trp Leu Arg Thr Lys Phe Ile Asp Arg Ile Glu Gly Asn 
145                 150                 155                 160 


Pro Met Gln Lys Lys Gln Glu Phe Met Leu Tyr Pro Lys Glu Asp Arg 
                165                 170                 175     


Asn Gln Leu Ile Tyr Glu Phe Ile Leu Arg Glu Leu Lys Lys Arg Tyr 
            180                 185                 190         


<210>  69
<211>  1625
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tetracycline gene

<400>  69
gttcaacaaa cgggccatat tgttgtataa gtgatgaaat actgaattta aaacttagtt       60

tatatgtggt aaaatgtttt aatcaagttt aggaggaatt aattatgaag tgtaatgaat      120

gtaacagggt tcaattaaaa gagggaagcg tatcattaac cctataaact acgtctgccc      180

tcattattgg agggtgaaat gtgaatacat cctattcaca atcgaattta cgacacaacc      240

aaattttaat ttggctttgc attttatctt tttttagcgt attaaatgaa atggttttga      300

acgtctcatt acctgatatt gcaaatgatt ttaataaacc acctgcgagt acaaactggg      360

tgaacacagc ctttatgtta accttttcca ttggaacagc tgtatatgga aagctatctg      420

atcaattagg catcaaaagg ttactcctat ttggaattat aataaattgt ttcgggtcgg      480

taattgggtt tgttggccat tctttctttt ccttacttat tatggctcgt tttattcaag      540

gggctggtgc agctgcattt ccagcactcg taatggttgt agttgcgcgc tatattccaa      600

aggaaaatag gggtaaagca tttggtctta ttggatcgat agtagccatg ggagaaggag      660

tcggtccagc gattggtgga atgatagccc attatattca ttggtcctat cttctactca      720

ttcctatgat aacaattatc actgttccgt ttcttatgaa attattaaag aaagaagtaa      780

ggataaaagg tcattttgat atcaaaggaa ttatactaat gtctgtaggc attgtatttt      840

ttatgttgtt tacaacatca tatagcattt cttttcttat cgttagcgtg ctgtcattcc      900

tgatatttgt aaaacatatc aggaaagtaa cagatccttt tgttgatccc ggattaggga      960

aaaatatacc ttttatgatt ggagttcttt gtgggggaat tatatttgga acagtagcag     1020

ggtttgtctc tatggttcct tatatgatga aagatgttca ccagctaagt actgccgaaa     1080

tcggaagtgt aattattttc cctggaacaa tgagtgtcat tattttcggc tacattggtg     1140

ggatacttgt tgatagaaga ggtcctttat acgtgttaaa catcggagtt acatttcttt     1200

ctgttagctt tttaactgct tcctttcttt tagaaacaac atcatggttc atgacaatta     1260

taatcgtatt tgttttaggt gggctttcgt tcaccaaaac agttatatca acaattgttt     1320

caagtagctt gaaacagcag gaagctggtg ctggaatgag tttgcttaac tttaccagct     1380

ttttatcaga gggaacaggt attgcaattg taggtggttt attatccata cccttacttg     1440

atcaaaggtt gttacctatg gaagttgatc agtcaactta tctgtatagt aatttgttat     1500

tacttttttc aggaatcatt gtcattagtt ggctggttac cttgaatgta tataaacatt     1560

ctcaaaggga tttctaaata tgatgaagaa agaccattcc aatcaggaat ggtctttttt     1620

tatgc                                                                 1625


<210>  70
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  70
tgcccctcta ttctgaaacc g                                                 21


<210>  71
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  71
accttccttt atgctttcga cg                                                22


