                         SEQUENCE LISTING

<110>  New Portal Limited
 
<120>  Bacteria for Targeting Tumors and Treating Cancer

<130>  N026.002.PCTCN

<150>  PCT/CN2017/101069
<151>  2017-09-08

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  306
<212>  DNA
<213>  Escherichia coli

<400>  1
atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta       60

cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt      120

ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc      180

tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg      240

gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga      300

atataa                                                                 306


<210>  2
<211>  219
<212>  DNA
<213>  Escherichia coli

<400>  2
atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat       60

gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt      120

gccgaacgct ggaaagcgga aaatcaggag gggatggctg aggtcgcccg gtttattgaa      180

atgaacggct cttttgctga tgagaacagg gactggtga                             219


<210>  3
<211>  164
<212>  DNA
<213>  Escherichia coli

<400>  3
taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt       60

aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt      120

atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaa                       164


<210>  4
<211>  1101
<212>  DNA
<213>  Escherichia coli

<400>  4
ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag tttacgtcca       60

gccaagctta ggatcccggg taccgatatc ggcagcatca cccgacggac tttgcgccga      120

ataaatacct gtgacggaag atcacttcgc agaataaata aatcctggtg tccctgttga      180

taccgggaag ccctgggcca acttttggcg aaaatgagac gttgatcggc acgtaagagg      240

ttccaacttt caccataatg aagtaagatc actaccgggc gtattttttg agttatcgag      300

attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga      360

tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac      420

ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa      480

gcacaagttt tatccggcct ttattcacat tcttgcccgc cagatgaatg ctcatccgga      540

attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta      600

caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga      660

tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc      720

ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag      780

tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac      840

catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctgacga ttcaggttca      900

tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg      960

cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttagatatc     1020

aagcttagga tccggaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt     1080

aggtccctcg aagaggttca c                                               1101


<210>  5
<211>  342
<212>  DNA
<213>  Escherichia coli

<400>  5
aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt       60

ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct      120

atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat      180

atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc      240

gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg      300

aaatgaacgg ctcttttgct gacgagaaca gggactggtg aa                         342


<210>  6
<211>  158
<212>  DNA
<213>  Escherichia coli

<400>  6
tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac       60

gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat      120

caaataaaac gaaaggctca gtcgaaagac tgggcctt                              158


<210>  7
<211>  2710
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleic acid system of one example embodiment

<400>  7
aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt       60

ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct      120

atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat      180

atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc      240

gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg      300

aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt taaggtttac      360

acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac      420

acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc      480

tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc      540

gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc      600

gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc      660

gttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta      720

ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta      780

cgtttctcgt tcagctttct tgtacaaagt ggtgatcaag cttgaaggta agcctatccc      840

taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt      900

ttaaacggtc tccagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag      960

attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg     1020

gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt     1080

gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca     1140

gtcgaaagac tgggccttaa taggcgtatc acgaggccct ttcgtcttca agaattccga     1200

tcatattcaa taacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt     1260

ctgaagagga gtttacgtcc agccaagctt aggatcccgg gtaccgatat cggcagcatc     1320

acccgacgga ctttgcgccg aataaatacc tgtgacggaa gatcacttcg cagaataaat     1380

aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc gaaaatgaga     1440

cgttgatcgg cacgtaagag gttccaactt tcaccataat gaagtaagat cactaccggg     1500

cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat     1560

cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt     1620

tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt     1680

aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg     1740

ccagatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg     1800

ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct     1860

ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc     1920

gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt     1980

ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa     2040

cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat     2100

gccgctgacg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct     2160

taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag     2220

ttattggtgc ccttagatat caagcttagg atccggaacc cttaatataa cttcgtataa     2280

tgtatgctat acgaagttat taggtccctc gaagaggttc actaatgcag ctggcacgac     2340

aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact     2400

cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg     2460

agcggataac aatttcacac aggaaatgtc tatgaagcag cgtattacag tgacagttga     2520

cagcgacagc tatcagttgc tcaaggcata tgatgtcaat atctccggtc tggtaagcac     2580

aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga     2640

ggggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg atgagaacag     2700

ggactggtga                                                            2710


<210>  8
<211>  2710
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid system of another example embodiment

<400>  8
aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt       60

ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct      120

atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat      180

atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc      240

gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg      300

aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt taaggtttac      360

acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac      420

acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc      480

tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc      540

gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc      600

gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc      660

gttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta      720

ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta      780

cgtttctcgt tcagctttct tgtacaaagt ggtgatcaag cttgaaggta agcctatccc      840

taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt      900

ttaaacggtc tccagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag      960

attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg     1020

gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt     1080

gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca     1140

gtcgaaagac tgggccttaa taggcgtatc acgaggccct ttcgtcttca agaattccga     1200

tcatattcaa taacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt     1260

ctgaagagga gtttacgtcc agccaagctt aggatcccgg gtaccgatat cggcagcatc     1320

acccgacgga ctttgcgccg aataaatacc tgtgacggaa gatcacttcg cagaataaat     1380

aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc gaaaatgaga     1440

cgttgatcgg cacgtaagag gttccaactt tcaccataat gaagtaagat cactaccggg     1500

cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat     1560

cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt     1620

tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt     1680

aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg     1740

ccagatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg     1800

ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct     1860

ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc     1920

gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt     1980

ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa     2040

cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat     2100

gccgctgacg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct     2160

taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag     2220

ttattggtgc ccttagatat caagcttagg atccggaacc cttaatataa cttcgtataa     2280

tgtatgctat acgaagttat taggtccctc gaagaggttc actaatgcag ctggcacgac     2340

aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact     2400

cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg     2460

agcggataac aatttcacac aggaaagcct tatgaagcag cgtattacag tgacagttga     2520

cagcgacagc tatcagttgc tcaaggcata tgatgtcaat atctccggtc tggtaagcac     2580

aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga     2640

ggggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg atgagaacag     2700

ggactggtga                                                            2710


<210>  9
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pelB leader sequence

<400>  9
atgaaatacc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcg       60

atggcc                                                                  66


<210>  10
<211>  5019
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exlA with pelB leader sequence

<400>  10
atgaaatacc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcg       60

atggcccata gagacaatcc tgtcttccac ctctcccccc ggggaaaact ccgttgcctg      120

atcgccggcc tgctgctggc gccccacctg ccccaggctt tcgccggcgg gctggaggcg      180

gccggcgggc cgggcggcac gccgcaactg ctcaaccagg gcggcgtgcc catcgtcaat      240

atcgtcgcgc cgaacgccgc cggcctgtcg cacaaccagt tcctcgacta caacgtcgac      300

cgccagggcc tggtcctcaa caatgcgctg caagccggcg cctcgcagct cgccggacaa      360

ctggcggcca acccgcagct gcgcggcgac gccgccagtg ccatcctcaa cgaagtgatc      420

agccgcaacg cctcggcgat caacggcccg caggaaatct tcggccaggc cgccgactac      480

atcctggcca accccaacgg catctcggtg aacggcggca gcttcatcaa cacgccccat      540

gccagcctgc tggtcggccg tccggaactg gccgacggca agctgcaggc gctgaacacc      600

aacgacgccg tcggcgccct gcagatccag ggccagggcc tgagcaaccg ggatggcagc      660

atcgccctgc tggcgccgcg agtcgacagc caaggcaaga tcgaggcgtc ggccgagctc      720

gacctgaccg tcggtcgcaa ccgcatcgac tacccgagcg gcaaggtcga gcgcgacccg      780

tccggcgacg tccgtccggg cgagcggcgc atcgacgcca gcctgttcgg cgccatgcag      840

gccggacgca tcaacatcct cagcaccgcc gagggcgccg gcgtgcgcgt cggcccggtc      900

gggatcgacg gcagggacgg cgtcgacctg cgctccgccg gcgacctgtc gatcagcggc      960

caggcgcttc ccgacaacag cctgaacgcc ttgcgcgcgg cgatccgcag cgacagcggc     1020

aacgtcggcc tgcatgcccg cggcgacctg agcctggccg ccgccgacgt cagcggcggc     1080

agggtcgacc tcaagtccgg gcgcaacctg accctgggca gcgtggaaag ccgcaacctg     1140

cgggaaagcc gcgagcgctg gagcaacagc accatcggca tcacctggga aacctacgac     1200

cgcacgcgta ccgtcaccga ttcgaagcag cacggcagcc ggatcgatgc ccgcgccgac     1260

gcgagcctgg ccgcgcgggg cgacagcgaa ctgagggcgg cgacggtcaa ggccggcgct     1320

accctgaaag tctccagcgg cggcgatacc cgtcttctcg ccgccaccga gacccgcacc     1380

gaacgcgacc agggcgcgca ccgcaagcac ctgtggaaag ccaactggga caagggcagc     1440

agcgagcaac gcagcgtggc cagcagcctc gaaggcgcgc gggtcgaact cggcggcggc     1500

cggcgcctga atctggaagg cgccgacgtg gccagccgcg gcgacctcga cctgcaagcc     1560

aagagcgtcg acatcggcag cgccagccgc agccacagca gccgcgacaa cagctactcc     1620

ggcgacctgg tcggcggcag cttcttcggc agccacggcg atggcgacag cggcaagacc     1680

ctgcaacagg gcagccgggt caaggccgac ggcgcactga cggtgaccgc cgatgcggtc     1740

gaggtgcgcg gcagccaggt acgcggcgcg cgcaaggccg aggtagtcag cgggaaaggc     1800

tcgctgcgca tcgacggcgt ggaggaaacc gcccacagca acagctacag caaggacagc     1860

aagttcttcg gcatcgccaa ggacgagagt cgccagcgca gcaaggacag cagcaaccgc     1920

gccagcgaag tacgctcgga cagcaacctg accctgcgca gcgccgccga catcgccatt     1980

cgcggctccc gggtcgaggc tggcggcgcc ctcgccgccg aggccaaggg caacctggag     2040

atagcctcgg cgcaggagcg ccacgacggc aacgacagcc gccacacccg cggcttcgac     2100

gcctatgccg gcgagcagac cccaggcagc cgccaatacc gcgccggggt gcgctaccag     2160

gaccagcgga ccagcgtccg ccgcgaggaa acccgcaaca gcggctccag cctgggcggc     2220

gcctccctgg ccgtgaaggc cggcggcgat ctcaccgtga agggggccga gctgaaggcc     2280

agcgcgggcg acgccagcct ctccgggaag aacgtcgccc tgctcgccga gcaggacagc     2340

aagacccgca gcagcgaaca gaccaccacc ggcggcggct tctactatac cggcggcctg     2400

gaccgtgccg gcagcggtat cgaagtcggc caccagcgga tcgacgagaa cgacgccgaa     2460

agccatgcgc gcaccagcca ggtgaacgcg acgggcaatc tcaggatcga cgccgcccag     2520

ggcagcctga cgacgcaggg cgcgcgcctg gaggccggcg acagcctggc ggtcgccgcc     2580

ggcacggtcg acaaccaggc cgcccgcgac agccagagca gccagcgcca cgacagcggc     2640

tggagcggcg acatcggcgc caacctcgag taccgcggca tcgcccggcc gatcgagaag     2700

gcggtcgaag gcgtcgccca gcgcaaggtc caccagcccg gcctgctcga caacctggag     2760

cagccgaacg tcggcgtcga cctcgagatc agccaccgcg acagccgcgg cgagcaacag     2820

gcgagccagg cgcaggtcag cagcttcgcg ggcggccagg tcgaactgaa ggtcggcgac     2880

gccctgcggg acgagggcac ccgttaccag gctcgcagcg gaggcctcct catcgacgcc     2940

gccaggcatg acgccagggc ggcggagaac acctccggca gccatgagca gagcctcgac     3000

gccaaggtcg gcgggcgcct ctacaccacc accggccagg acctgaacct gcgcctgagc     3060

ggcattggcg gcagcagcga gaacagcgcc agccagacca ccgcggtggt cggcgaatac     3120

gccgcgaagc agggcgtcga gatccgcctc ggcggagacg gcctctacca gggcagccgc     3180

ttcgacggcg gcgaagccgg ggtcaggctc agcgccggcg gcaacctggc cctggaacag     3240

gccaacgacc ggcagagcgc cagcagcgcc agcctgcgtg gcgacgccgc gttgagcggc     3300

ggcatggccc ccagcgccaa cggcaaaggg ctgaacgcca gcgccggcct gcaactcgac     3360

cacaaggccg gcgacagccg ggacagccag gcgcgggtcg ccgacatcca ggccaagggc     3420

gcggtggagc tgcgcagcgg cggcgatctg gtcctgcaag gcagcaatat cggcagcgcg     3480

gcagcgaaga ccggcgacat agtcctggcc gccggcggca agctcgacct gcaggccgcc     3540

cgcgatagcc accgggccgg gggaaacaac ctcggcggcg gcttcagcct aggcggcggc     3600

agcgttcgcg acgccgaaac gagcagcaag aacggcagcg tcagcggcaa cttcaacatc     3660

ggccgggtcg acgaggaacg tcacgcgctg aacggcggca acctgcacag cgcgaccaag     3720

gccagccttt ccagcgccgc agacgacgcc accgcggtac gcctgcaagg cactcgcatc     3780

gaagccgccc aggtcagcct cgaagccggc aacggcggca ttctccagga gtccgccgaa     3840

tccagcgagc ggcgcgacaa ctggggcgtg ctgctcggtg ccggggccaa cggcggcaag     3900

accaccggcg cgccgagcga ctaccggagc gactatgccg tccaggcccg cgccaaggtc     3960

gatgtcgatg tcctgcgcag ccagacccag ggcgacagcg tcatccaggc cgaccgggta     4020

atcctggcga gccagggcga cacccgcctg gagggcgcgc gcatcgacgc ggcacaggtg     4080

gacgggcgca tcggcggcga cctgcgggtg gagagccgcc aggaccgcgc ggagggcgtg     4140

aaggtcaacg tcgacgcgcg cctgggcgtg gagaagaacc agcccggcct ggtgaacaag     4200

ctggcgagca agaccggacc gttgaaggac aagctggaaa ccaaggccga gaatgctttc     4260

gacaagcacc gcggcaagtt ggagaacggc atcgaccgta atgtcgagcg gctcggcaag     4320

gccggggaca acctcctcgc caaagccgaa aaggccaagg agcgcctggg cgagaagctg     4380

gtccgcagcg gcagctacga ggtcaacccg gagccgcgcg gcgccttcgc cagcaagctg     4440

gacagggcca ggggctatct ggcggagaaa ggcgaagcgc tcggcgaccg gctgtccggc     4500

ctcaagcagc gcctgtcgcc gaacaagacc ggtagctatg tggtgaacga caagcagacg     4560

gccggcgcca aggtcggcaa tgccgccgag aacgtgctgt tcggcgacaa gagcggcgaa     4620

gcctcggtaa ccccgacgct gtacctggac gtcagccacg tcagccgcaa ctacgtcacc     4680

gaggcctccg gcatcaccgg caggcagggc gtgaacctgc aggtgggcgc agcgacccag     4740

ctgaccggcg cacggatcag cgccagcgac ggcaaggtcg acctcggcgg ctcgcgcgtg     4800

gaaacccgcg ccctggccgg caaggactac cgcgccgatc tcggcctgaa cgtctccagg     4860

tcgccggtgg acctggcctt cggtatcaag gacgagttca gccaggagca cgaccaggcg     4920

acccgcgacg accaggcctt caacctcggc gccctgcgcg tcggcggacg caaccgcgac     4980

cagcagttgc aggccggcat cgagcagaag gccgactaa                            5019


<210>  11
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nhe with pelB leader sequence

<400>  11
atgaaatacc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcg       60

atggccaata cttatgtaag ggaaccagtt aatgcattta ctcacttagg tggagcgata      120

ttatcattta ttgcgttatt agctatgctt gtgaaagttt ctatcaagat gccatcattt      180

gctgcaatta cagctgttat tttgtttggt attggaatga tggtccttta tacggcgtca      240

gctgtatatc atagtgttgt ggccaatgaa cgtgttatat acttctttag gaagctagat      300

cattctatga tttttatatt aattgcaggt acatatgcac ccttttgctt aattacatta      360

aattcagcaa gtggtttgct attattttgt ttagtctatg caactgcgat ttgtggcatt      420

gtatttaaaa tgttttggtt taattgtcca aggtggttat cgacagcaat ttatattacg      480

atgggttggt taattgtttt attctttgca ccgttagctg agaatttaag tacaggaggc      540

attattttct tagtacttgg tggcattttt tatacaattg gtggatttat ttatggaaca      600

aagccaaaat ggttagagtt taaatatatg gggcatcatg aaatttttca tgtttttgta      660

ttattaggta gtcttgcgca ttttctaagt gtatattgtt acgtaattta a               711


<210>  12
<211>  2220
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hlyA of Vibrio cholera with pelB leader sequence

<400>  12
atgaaatacc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcg       60

atggccgcaa atatcaatga accaagtggt gaagcggcgg atattattag tcaagtcgct      120

gatagtcatg caataaaata ttacaatgct gctgattggc aagccgaaga caacgcatta      180

ccgagcttag ctgagctgcg cgatttggtg attaaccagc aaaaacgcgt tttggttgat      240

ttcagtcaga tcagtgatgc tgaaggtcaa gcagagatgc aagcccaatt cagaaaggct      300

tatggggtgg gttttgctaa tcaatttatt gtcatcactg aacataaagg ggaactgctg      360

tttacacctt ttgatcaggc agaagaggtt gaccctcaat tactcgaagc gccgcgtacc      420

gctcgcttat tagcgcgctc tggttttgca agtccggcac cggcaaacag cgaaacaaat      480

accttgccgc atgtggcttt ttacatcagt gtcaaccgtg cgatcagcga tgaagagtgt      540

acctttaaca actcttggtt gtggaaaaac gaaaagggca gtcgtccgtt ctgtaaagat      600

gccaatatct cattgattta tcgagttaac ctagagcgtt cattgcaata cggcattgtg      660

ggttccgcga caccggatgc caaaattgtg cgtatcagcc tagatgatga cagcacggga      720

gccggcattc atctgaatga tcaactcggt tatcgtcagt ttggagccag ttatacgacg      780

ttagatgcct atttccgtga gtggtcaacc gatgcgattg cccaagatta tcgcttcgtg      840

tttaacgcat cgaacaataa agcgcagatc ctgaaaacct ttcctgtcga taacattaac      900

gagaaatttg agcgcaaaga ggtttcaggt tttgagcttg gggtgactgg tggggtggaa      960

gtcagtggag atggcccgaa agccaaacta gaggcgagag caagttatac ccagagtcgc     1020

tggttaacct acaacacaca agactatcgt attgagcgta atgcgaagaa tgcgcaagcg     1080

gttagcttta catggaatcg tcaacaatac gcgacagcag aatcgctact caatcgttcg     1140

accgatgctt tgtgggtgaa tacctacccg gtagatgtaa accgtattag cccgctgagc     1200

tacgcgagtt ttgtgccgaa aatggatgtg atttataaag cctcagccac agagacaggc     1260

agtacggatt ttatcatcga ctcttcggtc aatatccgcc caatctataa cggtgcttat     1320

aagcactact atgtggtcgg tgctcatcag ttctaccatg gctttgaaga taccccacgt     1380

cgtcgaatca cgaaatcggc aagctttacg gtcgattggg atcacccagt attcacgggt     1440

ggccgcccgg tcaacctaca acttgccagc tttaacaacc gctgtattca agtcgatgct     1500

caaggtcgct tggcggccaa tacgtgcgat agccagcaat cagcgcaatc gttcatctat     1560

gatcagcttg gtcgttatgt gagtgcgagt aacaccaagc tctgtcttga tggtgaggca     1620

ttagacgcat tgcaaccctg taaccaaaac ctgactcagc gttgggagtg gcgtaaaggc     1680

acagatgaat tgaccaatgt ctacagcggc gagtcccttg gacatgacaa acaaaccggt     1740

gagcttggtt tgtatgcgag cagcaacgat gcggtaagtt tacgtaccat caccgcttat     1800

accgatgtgt ttaatgcgca agaaagttcg ccgattctgg gttacacaca agggaaaatg     1860

aatcagcagc gtgtgggaca agatcatcgt ttgtatgtgc gagcgggtgc tgccattgat     1920

gcattagggt ccgcctccga tttattggtt ggtggcaatg gtggtagctt gagttcggtg     1980

gatctgtccg gcgtgaaatc catcacggca acctctggtg atttccaata tggcggtcag     2040

cagttggtgg cgctgacatt cacctaccaa gatggacgtc agcaaacggt aggctcgaaa     2100

gcgtatgtca ccaatgctca tgaagaccgt ttcgatttac cggctgccgc taagatcact     2160

caactgaaaa tttggtctga cgattggttg gtgaaagggg ttcaatttga tttgaactaa     2220


<210>  13
<211>  7410
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hlyBACD operon of E. coli

<400>  13
ttggtttgct tttttttacc tgccaccgca atgaatgctt tttttatgtt aatgtgcgtt       60

atgaaactaa atgcaagaaa catatttaaa ggattaatat cgttctctca cagactccgt      120

ttacttattc aagaatataa tttaatttat agtgagctta ttatgaatat gaacaatcca      180

ttagaggttc ttgggcatgt atcctggctc tgggccagtt ccccattaca cagaaactgg      240

ccagtctctt tgtttgcaat aaatgtatta cctgcaatac gggctaacca atatgcttta      300

ttaacccggg ataattaccc tgttgcatat tgtagttggg ctaatttaag tttagaaaat      360

gaaattaaat atcttaatga tgttacctca ttagtcgcag aagactggac ttcaggtgat      420

cgtaaatggt tcattgactg gattgctcct ttcggggata acggtgccct gtacaaatat      480

atgcgaaaaa aattccctga tgaactattc cgagccatca gggtggatcc caaaactcat      540

gttggtaaag tatcagaatt tcacggaggt aaaattgata aacagttagc gaataaaatt      600

tttaaacaat attaccacga gttaataact gaagtaaaaa acaagacaga tttcaatttt      660

tcattaacag gttaagagat aattaaatgc caacaataac cactgcacaa attaaaagca      720

cactacagtc tgcaaagcaa tcctctgcaa ataaattgca ctcagcagga caaagcacga      780

aagatgcatt aaaaaaagca gcagagcaaa cccgcaatgc gggaaacaga ctcattttac      840

ttatccctaa agattataaa ggacagggtt caagccttaa tgaccttgtc aggacggcag      900

atgaactggg aattgaagtc cagtatgatg aaaagaatgg cacggcgatt actaaacagg      960

tattcggcac agcagagaaa ctcattggcc tcaccgaacg gggagtgact atctttgcac     1020

cacaattaga caaattactg caaaagtatc aaaaagcggg taataaatta ggcggcagtg     1080

ctgaaaatat aggtgataac ttaggaaagg caggcagtgt actgtcaacg tttcaaaatt     1140

ttctgggtac tgcactttcc tcaatgaaaa tagacgaact gataaagaga caaaaatctg     1200

gtagcaatgt cagttcttct gaactggcaa aagcgagtat tgagctaatc aaccaactcg     1260

tggacacagc tgccagcatt aataataatg ttaactcatt ttctcaacaa ctcaataagc     1320

tgggaagtgt attatccaat acaaagcacc tgaccggtgt tggtaataag ttacagaatt     1380

tacctaacct tgataatatc ggtgcagggt tagatactgt atcgggtatt ttatctgcga     1440

tttcagcaag cttcattctg agcaatgcag atgcagatac cggaactaaa gctgcagcag     1500

gtgttgaatt aacaacgaaa gtactgggta atgttggaaa aggtatttct caatatatta     1560

tcgcacagcg cgctgcacag ggattatcta catctgctgc tgctgccggt ttaattgctt     1620

ctgtagtgac attagcaatt agtcccctct cattcctgtc cattgccgat aagtttaaac     1680

gtgccaataa aatagaggag tattcacaac gattcaaaaa acttggatac gatggtgaca     1740

gtttacttgc tgcttttcac aaagaaacag gagctattga tgcatcgtta acaacgataa     1800

gcactgttct ggcttcagta tcttcaggta ttagtgctgc tgcaacgaca tctctggttg     1860

gtgcaccggt aagcgcgctg gtaggggctg ttacggggat aatttcaggc atccttgagg     1920

cttcaaaaca ggcaatgttt gaacatgtcg ccagtaaaat ggccgatgtt attgctgaat     1980

gggagaaaaa acacggcaaa aattactttg aaaatggata tgatgcccgc catgctgcat     2040

ttttagaaga taactttaaa atattatctc agtataataa agagtattct gttgaaagat     2100

cagtcctcat tacccagcaa cattgggata cgctgatagg tgagttagcg ggtgtcacca     2160

gaaatggaga caaaacactc agtggtaaaa gttatattga ctattatgaa gaaggaaaac     2220

gtctggagaa aaaaccggat gaattccaga agcaagtctt tgacccattg aaaggaaata     2280

ttgacctttc tgacagcaaa tcttctacgt tattgaaatt tgttacgcca ttgttaactc     2340

ccggtgagga aattcgtgaa aggaggcagt ccggaaaata tgaatatatt accgagttat     2400

tagtcaaggg tgttgataaa tggacggtga agggggttca ggacaagggg tctgtatatg     2460

attactctaa cctgattcag catgcatcag tcggtaataa ccagtatcgg gaaattcgta     2520

ttgagtcaca cctgggagac ggggatgata aggtcttttt atctgccggc tcagccaata     2580

tctacgcagg taaaggacat gatgttgttt attatgataa aacagacacc ggttatctga     2640

ccattgatgg cacaaaagca accgaagcgg gtaattacac ggtaacacgt gtacttggtg     2700

gtgatgttaa gattttacag gaagttgtga aggagcagga ggtttcagtt ggaaaaagaa     2760

ctgaaaaaac gcaatatcgg agttatgaat tcactcatat caatggtaaa aatttaacag     2820

agactgataa cttatattcc gtggaagaac ttattgggac cacgcgtgcc gacaagtttt     2880

ttggcagtaa atttactgat atcttccatg gcgcggatgg tgatgaccat atagaaggaa     2940

atgatgggaa tgaccgctta tatggtgata aaggtaatga tacgctgagg ggcggaaacg     3000

gggatgacca gctctatggc ggtgatggca atgataagtt aattgggggg acaggtaata     3060

attaccttaa cggcggtgac ggagatgatg agcttcaggt tcaggggaat tctcttgcta     3120

aaaatgtatt atccggtgga aaaggtaatg acaagttgta cggcagtgag ggagcagatc     3180

tgcttgatgg cggagaaggg aatgatcttc tgaaaggtgg atatggtaat gatatttatc     3240

gttatctttc aggatatggc catcatatta ttgacgatga tggggggaaa gacgataaac     3300

tcagtttggc tgatattgat ttccgggatg tggccttcag gcgagaaggt aatgacctca     3360

tcatgtataa agctgaaggt aatgttcttt ccattggtca taaaaatggt attacattca     3420

ggaactggtt tgaaaaagag tcaggtgata tctctaatca ccagatagag cagatttttg     3480

ataaagacgg cagggtaatc acaccagatt cccttaaaaa ggcacttgag tatcaacaga     3540

gtaataataa ggcaagttat gtgtatggga atgatgcatt agcctatgga agtcaggata     3600

atcttaatcc attaattaat gaaatcagca aaatcatttc agctgcaggt aattttgatg     3660

ttaaagagga aagagctgca gcttctttat tgcagttgtc cggtaatgcc agtgattttt     3720

catatggacg gaactcaata actttgacag catcagcata atttattaat ttaaataata     3780

gcaatcttac tgggctgtgc cacataagat tgctattttt ttggagtcat aatggattct     3840

tgtcataaaa ttgattatgg gttatacgcc ctggagattt tagcccaata ccataacgtc     3900

tctgttaacc cggaagaaat taaacataga tttgacacag acgggactgg tctgggatta     3960

acgtcatggt tgcttgctgc gaaatcttta gaactaaagg taaaacaggt aaaaaaaaca     4020

attgaccgat taaactttat ttctttgccc gcattagtct ggagagagga tggacgtcat     4080

tttattctga ctaaagtcag taaagaagca aacagatatc ttatttttga tctggagcaa     4140

cgaaatcccc gtgttctcga acagtctgag tttgaggcgt tatatcaggg gcatattatt     4200

cttattgctt cccgttcttc tgttaccggg aaactggcaa aatttgactt tacctggttt     4260

atccctgcca ttataaaata cagaaaaata tttattgaaa cccttgttgt atctgttttt     4320

ttacaattat ttgcattaat aacccccctt ttttttcagg tggttatgga caaagtatta     4380

gtacacaggg ggttttcaac ccttaatgtt attactgtcg cattatctgt tgtggtggtg     4440

tttgagatta tactcagcgg tttaagaact tacatttttg cacatagtac aagtcggatt     4500

gatgttgagt tgggtgccaa actcttccgg catttactgg cgctaccgat ctcttatttt     4560

gagagtcgtc gtgttggtga tactgttgcc agggtaagag aattagacca gatccgtaat     4620

tttctgacag gacaggcatt aacatctgtt ctggacttat tattttcatt catatttttt     4680

gcggtaatgt ggtattacag cccaaagctt actctggtga tcttattttc gctgccctgt     4740

tatgctgcat ggtctgtttt tattagcccc attttgcgac gtcgccttga tgataagttt     4800

tcacggaatg cggataatca atctttcctg gtggaatcag tcacggcgat taacactata     4860

aaagctatgg cagtctcacc tcagatgacg aacatatggg acaaacaatt ggcaggatat     4920

gttgctgcag gctttaaagt gacagtatta gccaccattg gtcaacaagg aatacagtta     4980

atacaaaaga ctgttatgat catcaacctg tggttgggag cacacctggt tatttccggg     5040

gatttaagta ttggtcagtt aattgctttt aatatgcttg ctggtcagat tgttgcaccg     5100

gttattcgcc ttgcacaaat ctggcaggat ttccagcagg ttggtatatc agttacccgc     5160

cttggtgatg tgcttaactc tccaactgaa agttatcatg ggaaactggc attaccggaa     5220

attaatggta atatcacttt tcgtaatatc cggtttcgct ataagcctga ctctccggtt     5280

attttagata atatcaatct cagtattaag cagggggagg ttattggtat tgtcggacgt     5340

tctggttcag gaaaaagcac attaactaaa ttaattcaac gtttttatat tcctgaaaat     5400

ggccaggtct taattgatgg acatgatctt gcgttggccg atcctaactg gttacgtcgt     5460

caggtggggg ttgtgttgca ggacaatgtg ctgcttaatc gcagtattat tgataatatc     5520

tcactggcta atcctggtat gtccgtcgaa aaagttattt atgcagcgaa attagcaggc     5580

gctcatgatt ttatttctga attgcgtgag gggtataaca ccattgtcgg ggaacagggg     5640

gcaggattat ccggaggtca acgtcaacgc atcgcaattg caagggcgct ggtgaacaac     5700

cctaaaatac ttatttttga tgaagcaacc agtgctctgg attatgagtc ggagcatatc     5760

atcatgcgca atatgcacaa aatatgtaag ggcagaacgg ttataatcat tgctcatcgt     5820

ctgtctacag taaaaaatgc agaccgcatt attgtcatgg aaaaagggaa aattgttgaa     5880

cagggtaaac ataaggaact gctttctgaa ccggaaagtt tatacagtta cttatatcag     5940

ttacagtcag actaacagaa agaacagaag aatatgaaaa catggttaat ggggttcagc     6000

gagttcctgt tgcgctataa acttgtctgg agtgaaacat ggaaaatccg gaagcaatta     6060

gatactccgg tacgtgaaaa ggacgaaaat gaattcttac ccgctcatct ggaattaatt     6120

gaaacgccgg tatccagacg gccgcgtctg gttgcttatt ttattatggg gtttctggtt     6180

attgctgtca ttttatctgt tttaggtcag gtggaaattg ttgccactgc aaatgggaaa     6240

ttaacactaa gtgggcgcag caaagaaatt aaacctattg aaaactcaat agttaaagaa     6300

attatcgtaa aagaaggaga gtcagtccgg aaaggggatg tgttattaaa gcttacagca     6360

ctgggagctg aagctgatac gttaaaaaca cagtcatcac tgttacagac caggctggaa     6420

caaactcggt atcaaattct gagcaggtca attgaattaa ataaactacc tgaactgaag     6480

cttcctgatg agccttattt tcagaatgta tctgaagagg aagtactgcg tttaacttct     6540

ttgataaaag aacagttttc cacatggcaa aatcagaagt atcaaaaaga actgaatctg     6600

gataagaaaa gagcagagcg attaacaata cttgcccgta taaaccgtta tgaaaattta     6660

tcgagagttg aaaaaagccg tctggatgat ttcaggagtt tattgcataa acaggcaatt     6720

gcaaaacatg ctgtacttga gcaggagaat aaatatgtcg aggcagcaaa tgaattacgg     6780

gtttataaat cgcaactgga gcaaattgag agtgagatat tgtctgcaaa agaagaatat     6840

cagcttgtca cgcagctttt taaaaatgaa attttagaca agctaagaca aacaacagac     6900

aacattgagt tattaactct ggagttagag aaaaatgaag agcgtcaaca ggcttcagta     6960

atcagggccc ctgtttcggg aaaagttcag caactgaagg ttcatactga aggtggggtt     7020

gttacaacag cggaaacact gatggtcatc gttccggaag atgacacgct ggaggttact     7080

gctctggtac aaaataaaga tattggtttt attaacgtcg ggcagaatgc catcattaaa     7140

gtggaggcct ttccttacac ccgatatggt tatctggtgg gtaaggtgaa aaatataaat     7200

ttagatgcaa tagaagacca gaaactggga ctcgttttta atgtcattgt ttctgttgaa     7260

gagaatgatt tgtcaaccgg gaataagcac attccattaa gctcgggtat ggctgtcact     7320

gcagaaataa agactggaat gcgaagcgta atcagctatc ttcttagtcc tctggaagag     7380

tctgtaacag aaagtttaca tgagcgttaa                                      7410


